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2.
Chem Biol Drug Des ; 94(4): 1824-1834, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31293023

RESUMO

Due to the potencies in the treatments of cancer, infectious diseases, and autoimmune diseases, the developments of human TLR8 (hTLR8) agonists and antagonists have attracted widespread attentions. The hTLR8 agonists and antagonists have similar structures but with completely opposite biological effects. Up to date, the subtle differences in the structures between the hTLR8 agonists and antagonists are still unknown. In this work, emerging chemical pattern (ECP) was successfully used to extract the key chemical patterns of the hTLR8 agonists and antagonists. By using CAEP classifier, an optimal ECP model with only 3 descriptors was established with the overall prediction accuracy larger than 90%. Further hierarchical cluster analysis and molecular docking showed that the H-bond and hydrophobic properties are the key features distinguishing the hTLR8 agonists from antagonists. Comparing with the antagonists, the agonists show stronger specific H-bond properties, while antagonists have stronger non-specific hydrophobic properties. The significant differences in the structural properties may be closely related to the activation/inhibition mechanism of hTLR8.

3.
Oncol Lett ; 17(6): 5608-5618, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31186783

RESUMO

The aim of the present study was to analyze lung adenocarcinoma-associated microarray data and identify potentially crucial genes. The gene expression profiles were downloaded from the Gene Expression Omnibus database and 6 datasets, of which 2 were discarded and 4 were retained, were preprocessed using packages in the R computing language. Subsequently, Gene Set Enrichment Analysis (GSEA) and meta-analysis was used to screen the common pathways and differentially expressed genes at the transcriptional level. The genes detected from GSEA through The Cancer Genome Atlas databases were subsequently examined, and the crucial genes by survival data were identified. Pathways of the crucial genes were obtained using the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway of the online website Database for Annotation, Visualization and Integrated Discovery (DAVID) tool, and the pathways of crucial genes that were upregulated or downregulated were matched using the Venn method to identify the common crucial pathways. Furthermore, on the basis of the common crucial pathways, key genes that are closely associated with the development and progression of lung adenocarcinoma were identified with the KEGG pathway of DAVID. Additional information was obtained through Gene Ontology annotation. A total of two key pathways, including cell cycle and DNA replication, as well as 12 key genes [DNA polymerase δ subunit 2, DNA replication licensing factor MCM4, MCM6, mitotic checkpoint serine/threonine-protein kinase BUB1, BUB1ß, mitotic spindle assembly checkpoint protein MAD2A, dual specificity protein kinase TTK, M-phase inducer phosphatase 1, cell division control protein 45 homolog, cyclin-dependent kinase inhibitor 1C, pituitary tumor-transforming gene 1 protein and polo-like kinase 1] were identified. These key pathways and genes may be studied in future studies involving gene transfection/knockdown, which may provide insights into the prognosis of lung adenocarcinoma. Additional studies are required to confirm their biological function.

4.
Am J Transl Res ; 11(4): 2484-2495, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31105856

RESUMO

Acetabular labral tears are common in patients presenting with hip or groin pain. When the labrum is irreparable, labral reconstruction procedures are warranted. A circumferentially intact labrum is essential for joint health, whereas labral deficiency may predispose hip joints to osteoarthritis (OA). We aimed to determine whether labral reconstruction provides a benefit in terms of reduced OA development compared with labral resection. We performed labral reconstructions, labral resections, and sham operations in a porcine model. We assessed subsequent OA development by macroscopic observation, scanning electron microscopy, nanoindentation, histology, magnetic resonance imaging of cartilage in the acetabulum and femoral head, and enzyme-linked immunosorbent assays for inflammatory cytokines in the synovial fluid. We subjected the postoperative implants to biomechanical, histological, and mRNA expression analyses. At 24 weeks after surgery, the resected joints displayed apparent degenerative changes, in which the labral defects were filled with tiny amounts of loose, fibrous scar tissue. Compared with the resected joints, the reconstructed joints showed smooth and homogeneous geomorphology of cartilage surfaces; increased cartilage extracellular matrix (type II collagen and proteoglycans) content, elastic modulus, and hardness; and decreased type X collagen content, macroscopic and histological pathology scores, and inflammatory cytokines in the synovial fluid. The postoperative implants had compression, tensile, and histological features similar to those of the native labrum, which may have helped to attenuate OA development following labral reconstruction. Labral reconstruction using autografts greatly reduced OA progression compared with labral resection. The autologous implants used for reconstruction effectively restored the biomechanical and histological features of the labrum, contributing to hip joint homeostasis.

5.
Integr Biol (Camb) ; 2019 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-30855664

RESUMO

Binding/unbinding kinetics are key determinants of drug potencies. However, there are still a lot of challenges in predicting kinetic properties during early-stage drug development. In this work, position-restrained molecular dynamics simulations combined with energy decomposition were applied to extract protein-ligand interaction (PLI) fingerprints along the unbinding pathway of 20 p38 mitogen-activated protein kinase (p38 MAPK) Type II inhibitors. The results showed that the electrostatic and/or van der Waals interaction fingerprints at three key positions can be used for accurate prediction of the dissociation rate constants (koff) of p38 MAPK Type II inhibitors. The strategy proposed in this paper can provide not only an efficient method of predicting the dissociation rates of the p38 MAPK Type II inhibitors, but also the atom-level mechanism of enthalpy-driven unbinding process.

6.
Knee Surg Sports Traumatol Arthrosc ; 27(11): 3668-3678, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30923857

RESUMO

PURPOSE: Cartilage repair presents a challenge to clinicians and researchers. A more effective procedure that can produce hyaline-like cartilage is needed for articular cartilage repair. Mosaic osteochondral grafts for large osteochondral defects often show poor integration between the grafts and the surrounding normal cartilage, leading to defective cracks filled with fibrous tissue instead of hyaline-like cartilage. In the present study, we aimed to repair the defective cracks with a calcium alginate (CaAlg) hydrogel containing bone morphogenetic protein 4 (BMP4)-enhanced adipose-derived stem cells (ADSCs). METHODS: ADSCs were transduced with BMP4 (B-ADSCs). The expression of BMP4 and type II collagen was confirmed using an enzyme-linked immunosorbent assay (ELISA). Swine models of large cartilage defects of the knee were constructed and received one of the four treatments: mosaicplasty only, mosaicplasty with the CaAlg hydrogel, mosaicplasty with the CaAlg hydrogel containing ADSCs, or mosaicplasty with the CaAlg hydrogel containing B-ADSCs injected into the defective cracks. Outcomes were evaluated at 12 and 24 weeks after surgery. RESULTS: The in vitro study showed that the osteogenic and chondrogenic activities of the B-ADSCs were enhanced compared with those of the control. In vivo, in the group that received mosaicplasty-containing B-ADSCs, osteochondral tissue was completely integrated with an intact surface. Additionally, the histological scores of the mosaicplasty-containing B-ADSCs group were significantly higher than those of the other groups. Biomechanical examination confirmed that the neocartilage possessed properties similar to those of normal cartilage. CONCLUSIONS: Mosaicplasty and hydrogel containing B-ADSCs promoted the repair of large cartilage defects by regenerating hyaline cartilage and repairing dead spaces between osteochondral grafts and donor-site defects, thus improving the feasibility and success rate of one-stage complete repair surgery for large osteochondral defects. This proposed method provides a novel and effective means for the repair of large articular osteochondral defects.


Assuntos
Tecido Adiposo/citologia , Alginatos , Proteína Morfogenética Óssea 4 , Cartilagem Articular/cirurgia , Hidrogéis , Articulação do Joelho/cirurgia , Células-Tronco , Animais , Cartilagem Articular/fisiologia , Humanos , Cartilagem Hialina/fisiologia , Articulação do Joelho/fisiologia , Modelos Animais , Osteogênese , Regeneração , Suínos
7.
Knee Surg Sports Traumatol Arthrosc ; 27(9): 2877-2883, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30903219

RESUMO

PURPOSE: Clinical outcomes between reattachment of the superior peroneal retinaculum (SPR) and the bone block procedure were compared in this study to elucidate which procedure was safer and more effective. METHODS: From 2012 to 2016, 25 patients with recurrent peroneal tendon dislocation underwent the bone block procedure (group A), and another 22 patients underwent reattachment of the SPR (group B). American Orthopaedic Foot and Ankle Society (AOFAS) ankle-hindfoot score, Ankle Activity Score (AAS), time to return to sports activity, rate of return to sports level, range of motion (ROM) of the ankle, rate of recurrence, and overall patient satisfaction were collected to evaluate outcomes between the two groups. RESULTS: In group A, 24 patients followed up at a mean period of 42.5 ± 16.7 months. The mean postoperative AOFAS score was 92.9 ± 3.9. The median time to return to sports activity was 6.0 months (IQR 4.3-6.0 months) with 19 patients (79.2%) returning to their previous sports level. Two patients experienced recurrent dislocation, and 22 patients (91.7%) were satisfied with the procedure. In group B, 20 patients followed up at a mean period of 35.8 ± 15.3 months. The mean postoperative AOFAS score was 95.0 ± 4.2. The median time to return to sports activity was 5.0 months (IQR 4.0-5.0 months) with 18 patients (90.0%) returning to their previous sports level. No recurrence was reported, and 18 patients (90.0%) were satisfied with the procedure. The time to return to sports activity in group B was significantly shorter than that in group A. There was no significant difference in complications or clinical outcomes between the two procedures. CONCLUSION: Both procedures offered satisfactory results for recurrent peroneal tendon dislocation with low rates of recurrence and complications. However, the time to return to sports activity after the reattachment of the SPR was shorter than that after the bone block procedure. LEVEL OF EVIDENCE: Retrospective Comparative Study, Level III.


Assuntos
Traumatismos do Tornozelo/cirurgia , Luxações Articulares/cirurgia , Procedimentos Ortopédicos/métodos , Traumatismos dos Tendões/cirurgia , Tendões/cirurgia , Adulto , Tornozelo , Traumatismos do Tornozelo/psicologia , Articulação do Tornozelo/cirurgia , Fáscia , Feminino , , Humanos , Masculino , Satisfação do Paciente , Período Pós-Operatório , Amplitude de Movimento Articular , Recidiva , Estudos Retrospectivos , Volta ao Esporte , Esportes , Traumatismos dos Tendões/psicologia , Resultado do Tratamento , Adulto Jovem
8.
J Thorac Dis ; 10(10): 5714-5726, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30505479

RESUMO

Background: Esophageal squamous cell carcinoma (ESCC) is a common malignancy with high mortality. Because of the lack of clarity in the relevant genes and mechanisms involved, and the current difficulty for oncotherapy in providing therapeutic solutions, there is an urgent need to study this matter. While gene probe studies have been used to select the most virulent genes and pathways, paucity of case controls during gene screening and lack of conclusive results to expound the etiology and pathogenesis of the disease, have reduced study reliability. Methods: We chose six datasets from independent studies in the Gene Expression Omnibus (GEO) database and used gene set enrichment analysis and meta-analysis to select key genes and pathways. Results: We found four down-regulated and four up-regulated pathways through gene set enrichment analysis, and 406 differential genes through meta-analysis. Based on The Cancer Genome Atlas (TCGA), 995 differentially expressed genes were screened out. Comparing the 406 gene set with the 995 gene set, we found 19 common genes, of which 6 had a common pathway and were screened out as key genes regulating and controlling the prognosis of ESCC. Conclusions: Among the 19 genes, we found three genes that affect the chemotherapy of ESCC: BUB1B, BUB1, and TTK. Another three genes NDC1, NUP107, and NUP155 on the RNA transport pathway were also found. Altogether, these six genes are not only crucial in the development of ESCC, but also determine the prognosis of patients. The key genes and pathways identified in the present study will be used for the next stage in our study, which will involve gene elimination and other experimentation methods.

9.
Knee Surg Sports Traumatol Arthrosc ; 26(10): 3129-3134, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-29138920

RESUMO

PURPOSE: The purpose of the study was to evaluate the effect of concurrent arthroscopic osteochondral lesion (OCL) treatment and lateral ankle ligament repair on the outcome of chronic lateral ankle instability. It was hypothesized that the arthroscopic OCL treatment might have some negative effect on the outcome of chronic lateral ankle instability (CLAI) by compromising the rehabilitation program. METHODS: Ankle arthroscopy and anatomic lateral ankle ligament repair with suture anchors were performed for 70 patients with CLAI between 2010 and 2012. Thirty-four patients (group A), 20 males and 14 females with a median age of 30(14-54) years, received arthroscopic abrasion, curettage, drilling, or microfracture for OCLs. The splint was removed daily for joint motion exercises beginning at post-operative 2 weeks and full weight bearing was allowed between post-operative week 8 and 12. The other 36 patients (group B) with no combined OCL were followed up as controls. Pre-operative and post-operative visual analog scale (VAS) scores, American Orthopaedic Foot and Ankle Society (AOFAS) scores, Tegner scores, sprain recurrence, ankle stability, and range of motion (ROM) were evaluated and compared. RESULTS: The median follow-up was 46.5 (38-55) months and 44.5 (38-56) months for group A and group B, respectively. The median post-operative VAS score, AOFAS score, and Tegner score were improved from the pre-operative level for both groups with good-to-excellent results for more than 90% patients. No significant difference was found between the two groups for the subjective scores and satisfaction rate (n.s.). Recurrent sprain was found among nine patients(26.5%) of the group A and five patients (13.9%) of the group B (n.s.). The incidence of the ROM restriction of group A was significantly higher than in group B (23.5 vs 5.6%, P = 0.043). CONCLUSIONS: The concurrent arthroscopic treatment of OCL with lateral ankle ligament repair demonstrated no substantial negative effect on the overall mid-term outcome of the patients with CLAI except for a potential risk of ROM restriction. LEVEL OF EVIDENCE: III.


Assuntos
Artroscopia , Instabilidade Articular/cirurgia , Ligamentos Laterais do Tornozelo/cirurgia , Âncoras de Sutura , Adolescente , Adulto , Tornozelo , Traumatismos do Tornozelo/diagnóstico , Traumatismos do Tornozelo/cirurgia , Articulação do Tornozelo/fisiopatologia , Articulação do Tornozelo/cirurgia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Período Pós-Operatório , Amplitude de Movimento Articular , Entorses e Distensões/diagnóstico , Resultado do Tratamento , Suporte de Carga
10.
Food Chem ; 221: 1797-1804, 2017 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-27979164

RESUMO

Novel nano-sized dummy-surface molecularly imprinted polymers (DSMIPs) on a magnetic graphene oxide (GO-Fe3O4) surface were developed as substrates, using propionamide as a dummy template molecule for the selective recognition and rapid pre-concentration and removal of acrylamide (AM) from food samples. These products showed rapid kinetics, high binding capacity (adsorption at 3.68mg·g-1), and selectivity (imprinting factor α 2.83); the adsorption processes followed the Langmuir-Freundlich isotherm and pseudo-second-order kinetic models. Excellent recognition selectivity toward acrylamide was achieved compared to structural analogs, such as propionic and acrylic acids (selectivity factor ß 2.33, and 2.20, respectively). Moreover, DSMIPs-GO-Fe3O4 was used to quantify acrylamide in food samples, yielding satisfactory recovery (86.7-94.3%) and low relative standard deviation (<4.85%). Thus, our DSMIPs-GO-Fe3O4-based procedure was demonstrated to be a convenient and practical method for the separation, enrichment, and removal of acrylamide from food samples.


Assuntos
Acrilamida/análise , Grafite/química , Temperatura Alta , Impressão Molecular/métodos , Polímeros/química , Adsorção , Manipulação de Alimentos
11.
Am J Sports Med ; 44(4): 1031-9, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26838935

RESUMO

BACKGROUND: Acetabular labral reconstruction with autologous tendons is the preferred method for treating a severely damaged labrum. However, the healing process of implants remains unknown. Similar to the human acetabular labrum, the porcine acetabular labrum is a fibrocartilage-like tissue. PURPOSE: This study aimed to characterize the histological healing process and gene expression profile of implants in a porcine model of acetabular labral reconstruction. STUDY DESIGN: Descriptive laboratory study. METHODS: Eighteen pigs were included in this study. The pigs underwent unilateral acetabular labral reconstruction. A 1.0 cm-long defect was made at the site of the anterior (cranial) dorsal labrum, which was repaired using an autologous mesogluteus tendon. The pigs were sacrificed at 12 and 24 weeks postoperatively. The implants were subjected to histological assessment and gene expression analysis. The cell phenotype of the implants was visualized using paraffin-embedded sections. RESULTS: Macroscopic observations revealed that at 12 weeks, 8 of 9 implants partially filled the labral defect; by contrast, at 24 weeks, 6 of 9 implants fully filled and 3 implants partially filled the labral defects. Oval- or round-shaped fibrochondrocytes were found in the implants at 12 and 24 weeks. The matrix staining results showed that proteoglycan and collagen types 1 and 2 were more evident in the implants at 24 weeks than at 12 weeks. Gene expression analysis results revealed that COL2A1 and COL3A1 were expressed by the implants to a higher extent at 24 weeks than at 12 weeks; COL2A1 and COL3A1 were also expressed to a higher extent in the implants than in the native tendon. CONCLUSION: On the basis of the results of histological assessment and gene expression analysis, autologous tendon tissue for acetabular labral reconstruction can fully or partially fill labral defects and converts to fibrocartilage, which is rich in proteoglycan and collagen types 1 and 2, at 24 weeks in a porcine model. CLINICAL RELEVANCE: Autologous tendon tissue can be considered as a viable option for acetabular labral reconstruction.


Assuntos
Acetábulo/cirurgia , Cartilagem Articular/cirurgia , Tendões/transplante , Cicatrização , Animais , Condrócitos/metabolismo , Colágeno Tipo II/genética , Colágeno Tipo II/metabolismo , Colágeno Tipo III/genética , Colágeno Tipo III/metabolismo , Expressão Gênica , Articulação do Quadril/cirurgia , Modelos Animais , Suínos , Tendões/metabolismo , Transplante Autólogo
12.
Histol Histopathol ; 25(2): 197-203, 2010 02.
Artigo em Inglês | MEDLINE | ID: mdl-20017106

RESUMO

Human Papillomavirus (HPV)-associated esophageal carcinoma (EC) is a high incidence tumor worldwide. Dendritic cell (DC)-based tumor vaccine is considered an alternative therapy to treat EC. Here we developed a DC-based vaccine by transfecting cord blood CD34+ stem cell-derived DC with HPV18E7 gene, observed its biological characteristics and the antigen-specific T-cell cytotoxicity on EC cells induced by HPV18E7-DC in vitro. Our results showed that 1) HPV18E7 gene transfer did not change the typical morphology of mature DC, 2) the representative phenotypes of mature DC (CD80, CD86, and CD83) were highly expressed in HPV18E7- DC (81.6%, 80.5%, and 86.6%, respectively), 3) the expression level of 18E7 protein in HPV18E7-DC was 47.5%, and 4) the specific cytotoxicity against EC cells was significantly higher than that in controls (p<0.01). This study indicates the possibility of a DC-based immunotherapy in HPV-associated EC.


Assuntos
Vacinas Anticâncer , Carcinoma/imunologia , Proteínas de Ligação a DNA/imunologia , Células Dendríticas/imunologia , Neoplasias Esofágicas/imunologia , Células-Tronco Fetais/imunologia , Ativação Linfocitária , Proteínas Oncogênicas Virais/imunologia , Linfócitos T Citotóxicos/imunologia , Antígenos CD/análise , Antígenos CD34/análise , Antígeno B7-1/análise , Antígeno B7-2/análise , Carcinoma/genética , Carcinoma/virologia , Linhagem Celular Tumoral , Proliferação de Células , Forma Celular , Técnicas de Cocultura , Proteínas de Ligação a DNA/biossíntese , Proteínas de Ligação a DNA/genética , Neoplasias Esofágicas/genética , Neoplasias Esofágicas/virologia , Sangue Fetal/citologia , Sangue Fetal/imunologia , Citometria de Fluxo , Humanos , Imunoglobulinas/análise , Imunofenotipagem , Glicoproteínas de Membrana/análise , Proteínas Oncogênicas Virais/biossíntese , Proteínas Oncogênicas Virais/genética , Transfecção
13.
Chin Med J (Engl) ; 122(6): 706-11, 2009 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-19323939

RESUMO

BACKGROUND: In clinical studies there is still a lot of controversy about the increased anterior and rotational stability between double-bundle (DB) and single-bundle (SB) anterior cruciate ligament (ACL) reconstruction. The aim of this study was to evaluate the clinical results of four-tunnel DB ACL reconstruction. METHODS: Sixty-four consecutive patients with ACL ruptures from May 2005 to May 2006 were randomly assigned into two groups: 32 cases for SB ACL reconstruction and 32 cases for DB ACL reconstruction. Clinical data, including KT 2000, Biodex test, Lysholm score, Tegner score and IKDC score, were prospectively collected until at least 10 months post-operative. RESULTS: The average values of KT 2000 were (1.47 +/- 1.17) mm and (1.68 +/- 1.14) mm for the SB and DB ACL reconstruction groups at 30 degrees of knee flexion (P > 0.05), and were (1.04 +/- 0.98) mm and (1.13 +/- 0.98) mm at 90 degrees of knee flexion (P > 0.05). There were also no significant differences in Lysholm score, Tegner score, IKDC score and Biodex test scores between the two groups (P > 0.05). The operation time of DB ACL reconstruction was 20 minutes longer than the SB ACL reconstruction (P < 0.05). CONCLUSION: Double bundle ACL reconstructions have no obvious clinical advantages over single bundle ACL reconstructions.


Assuntos
Lesões do Ligamento Cruzado Anterior , Ligamento Cruzado Anterior/cirurgia , Procedimentos Ortopédicos/métodos , Procedimentos Cirúrgicos Reconstrutivos/métodos , Tendões/transplante , Adolescente , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Resultado do Tratamento , Adulto Jovem
14.
Ophthalmic Physiol Opt ; 29(1): 41-8, 2009 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-19154279

RESUMO

PURPOSE: To evaluate the effectiveness of progressive addition lenses (PALs), with a near addition of +1.50 D, on the progression of myopia in Chinese children. METHODS: We enrolled 178 Chinese juvenile-onset acquired myopes (aged 7-13 years, -0.50 to -3.00 D spherical refractive error), who did not have moderately or highly myopic parents, for a 2-year prospective study. They were randomly assigned to the PAL group or single vision (SV) group. Primary measurements, which included myopia progression and ocular biometry, were performed every 6 months. Treatment effect was adjusted for important covariates, by using a multiple linear regression model. RESULTS: One hundred and forty-nine subjects (75 in SV and 74 in PAL) completed the 2-year study. The myopia progression (mean +/- S.D.) in the SV and PAL groups was -1.50 +/- 0.67 and -1.24 +/- 0.56 D, respectively. This difference of 0.26 D over 2 years was statistically significant (p = 0.01). The lens type (p = 0.02) and baseline spherical equivalent refraction (p = 0.05) were significant contributing factors to myopia progression. Mean increase in the depth of vitreous chamber was 0.70 +/- 0.40 and 0.59 +/- 0.24 mm, respectively. This difference of 0.11 mm was statistically significant (p = 0.04). Age (p < 0.01) was the only contributing factor to the elongation of vitreous chamber. Different near phoria (p < 0.01) and gender (p = 0.02) caused different treatment effects when wearing SV lenses. However, there were no factors found to influence the treatment effect of wearing PALs. CONCLUSIONS: Compared with SV lenses, myopia progression was found to be retarded by PALs to some extent in Chinese children without moderately or highly myopic parents, especially for subjects with near esophoria or females.


Assuntos
Óculos , Miopia/fisiopatologia , Miopia/terapia , Adolescente , Criança , China/epidemiologia , Progressão da Doença , Feminino , Humanos , Modelos Lineares , Masculino , Miopia/etnologia , Fatores Sexuais , Resultado do Tratamento
15.
World J Gastroenterol ; 10(7): 934-9, 2004 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-15052669

RESUMO

AIM: To investigate the immune function of dendritic cells from both peripheral blood and operated tissues of esophageal carcinoma patients in order to find the relationship between the immune function of dendritic cells and the pathogenesis of esophageal carcinoma. METHODS: The expression of CD83, CD80, and CD86 on the surface of dendritic cells cultured from the peripheral blood of patients was detected compared with that from health donors using flow cytometry. The ability of dendritic cells to induce T lymphocyte proliferation was evaluated by a liquid scintillation counter. The expression of CD80, CD86, CD83, and S-100 proteins was assessed in esophageal carcinoma tissues using immunohistochemical method. RESULTS: Compared with those from healthy donors, dendritic cells cultured from the peripheral blood of patients expressed lower CD80 and CD86. Furthermore, the ability of dendritic cells in patients to induce T lymphocyte proliferation was significantly lower than that of the control group. Compared with the control group, the positive expression ratio and frequencies of CD80, CD86, and S-100 in esophageal carcinoma tissues were significantly down regulated. The expression of CD83 was up-regulated in the pericancerous tissues, but no expression was found in the cancerous nodules. CONCLUSION: The impaired immune function and the decreased number of dendritic cells cause pathogenesis and progression of esophageal carcinoma.


Assuntos
Carcinoma/imunologia , Células Dendríticas/imunologia , Neoplasias Esofágicas/imunologia , Idoso , Antígenos CD/metabolismo , Carcinoma/genética , Células Dendríticas/metabolismo , Neoplasias Esofágicas/genética , Humanos , Sistema Imunitário/fisiopatologia , Teste de Cultura Mista de Linfócitos , Pessoa de Meia-Idade , Fenótipo , Proteínas S100/metabolismo
16.
Cell Mol Immunol ; 1(3): 224-8, 2004 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-16219172

RESUMO

To investigate the expression of apoptosis-related protein (Fas, FasL, and Bcl-2) in the pathogenesis of autoimmune thyroid disorders (ATDs), immunohistochemical staining was performed on 20 Hashimoto's thyroiditis (HT), 20 Graves' disease (GD), and 20 thyroid follicular adenoma (TFA, as control). All the cases expressed Fas, mainly on the cell surface and cytoplasm. FasL was found in 17 cases of the TFA. Bcl-2 was detected in 15 cases of HT, 19 of GD and 17 of TFA. In TFA, a moderate Fas expression and a minimal or no FasL expression was detected on follicular cells. In HT, the follicles adjacent to infiltrating lymphocytes showed increased levels of Fas and FasL expression. A weaker staining of Fas and FasL was exhibited on infiltrating lymphocytes than on thyrocytes. In a comparison of GD with HT, thyrocytes and lymphocytes showed similar Fas staining, but for FasL the staining was rather weaker in HT. The expression of Bcl-2 was nearly identical in GD and TFA, but much weaker on the follicular cells in vicinity of lymphocytes and on the lymphocytes located in germinal centers of HT tissues. The expression of Fas, FasL, Bcl-2 in Hashimoto's thyroiditis and Graves' disease were almost same. FasL strong expression and Bcl-2 weak expression on the follicles in HT may induce apoptosis. These results provided evidence for expression of Fas, FasL and Bcl-2 in the pathogenesis of autoimmune thyroid disease. The lymphocytes seem not to be directly engaged in the process via their own FasL, but they may provide some cytokines that, in turn, upregulate Fas and/or FasL expression to induce apoptosis.


Assuntos
Regulação da Expressão Gênica , Doença de Graves/metabolismo , Glicoproteínas de Membrana/biossíntese , Proteínas Proto-Oncogênicas c-bcl-2/biossíntese , Glândula Tireoide/metabolismo , Receptor fas/biossíntese , Adulto , Proteína Ligante Fas , Feminino , Doença de Graves/patologia , Humanos , Masculino , Pessoa de Meia-Idade , Glândula Tireoide/patologia
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