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1.
Oncol Lett ; 18(4): 4194-4202, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31579421

RESUMO

Interaction between endoplasmic reticulum (ER) stress and oxidative stress contributes to the occurrence and development of various types of cancer. The X-box-binding protein 1 (XBP1), which is an important transcription factor in ER stress-related pathways, has also been reported to serve a protective role against oxidative stress. However, the role of XBP1 in serous ovarian cancer (SOC) remains elusive. The aim of the present study was to explore the biological function of XBP1 in SOC cells under normal or oxidative stress conditions. The expression of XBP1 was downregulated in the SOC cell lines A2780 and HO8910 by lentivirus-mediated short hairpin RNA (shRNA). Cell proliferative ability was evaluated by cell colony formation and viability assays. The sensitivity of ovarian cancer cells to oxidative stress was evaluated using cell survival rate and apoptotic rate, determined by the Cell Counting Kit-8 assay and flow cytometry, respectively. Reactive oxygen species (ROS) levels were measured by flow cytometry and cell immunofluorescence using a dichlorodihydrofluorescein diacetate probe. The mRNA and protein expression levels were detected by fluorescence quantitative polymerase chain reaction and western blot analysis, respectively. The results demonstrated that XBP1 was overexpressed in SOC compared with normal ovarian epithelial cells, and that downregulation of XBP1 significantly reduced cell proliferative ability. In addition, the downregulation of XBP1 significantly enhanced the sensitivity of SOC cells to H2O2 by increasing the intracellular ROS levels. The phosphorylation level of the mitogen-activated protein kinase (MAPK) p38 decreased in the cells of the XBP1-knockdown group. These results indicated that XBP1 may serve a protective role against oxidative stress in SOC cells, and the underlying molecular mechanism may be associated with the downregulation of phosphorylated p38. Therefore, targeting XBP1 may act synergistically with ROS inducers in the treatment of SOC.

2.
Nanoscale ; 11(36): 16860-16867, 2019 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-31482914

RESUMO

Electrochemiluminescence (ECL) nanomaterials are usually deposited compactly on the surface of electrodes, which may cause poor mass transfer of reactants, thereby resulting in low ECL efficiency. In this work, we developed a novel kind of luminescent material denoted as C-Au-luminol nanospheres (C-Au-Lum NSs) by high dispersion of luminophores on porous carbon nanospheres (PCNSs). C-Au-Lum NSs were facilely prepared by the in situ reduction of chloroauric acid with the luminescent reagent luminol (Lum) on the nano-pores of PCNSs. Plenty of luminescent Au-Lum NPs were dispersedly concentrated inside the numerous pores and hollow interiors of PCNSs, effectively increasing the mass transfer of reagents and accelerating the electron transport inside the porous nanospheres. This greatly improved the availability of luminophores and endowed C-Au-Lum NSs with excellent ECL emission. After further integrating with enzymatic circulation and strand displacement, an ultrasensitive ECL biosensor was achieved for the ultrasensitive detection of an important tumor biomarker, mucin1. The logarithmically linear range from 0.1 pg mL-1 to 1 ng mL-1 with the detection limit of 47.6 fg mL-1 (S/N = 3) was achieved, demonstrating the superior performance of C-Au-Lum NSs. This work would provide new ideas for the construction of high-performance ECL sensing platforms for diverse applications.

3.
Chem Sci ; 10(25): 6295-6303, 2019 Jul 07.
Artigo em Inglês | MEDLINE | ID: mdl-31341582

RESUMO

Flexible and wearable chemical sensors show great capability and potential in retrieving physiologically related chemical or biochemical information from elastic and curvilinear living bodies. However, so far, no flexible electrochemiluminescence (ECL) device has been reported, though ECL measurements have been extensively investigated and widely applied in many fields. Herein, we for the first time designed and fabricated a flexible ECL sensor by immobilizing highly luminescent nanospheres on Au nanotube (Au NT) networks, and subsequently coating an elastic molecularly imprinted polymer (MIP) thereon. The as-prepared flexible ECL platform displayed successive and desirable mechanical compliance while generating a very stable ECL signal during deformation, facilitating highly selective detection of physiologically relevant chemicals from bodies. On-body wearable sampling and subsequent detection of lactate and urea from sweat showed the ECL performance of this sensor displaying desirable fidelity, reusability and high stability against disturbance. This work successfully incorporated the ECL sensing model into a flexible and wearable device, therefore providing a promising new path for non-invasively monitoring the products of metabolism for health care and biomedical investigations.

4.
Gene ; 709: 56-64, 2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31108164

RESUMO

PURPOSE: In order to identify the molecular characteristics and improve the efficacy of early diagnosis of mucinous epithelial ovarian cancer (mEOC), here, the transcriptome profiling by weighted gene co-expression network analysis (WGCNA) has been proposed as an effective method. METHODS: The gene expression dataset GSE26193 was reanalyzed with a systematical approach, WGCNA. mEOC-related gene co-expression modules were detected and the functional enrichments of these modules were performed at GO and KEGG terms. Ten hub genes in the mEOC-related modules were validated using two independent datasets GSE44104 and GSE30274. RESULTS: 11 co-expressed gene modules were identified by WGCNA based on 4917 genes and 99 epithelial ovarian cancer samples. The turquoise module was found to be significantly associated with the subtype of mEOC. KEGG pathway enrichment analysis showed genes in the turquoise module significantly enriched in metabolism of xenobiotics by cytochrome P450 and steroid hormone biosynthesis. Ten hub genes (LIPH, BCAS1, FUT3, ZG16B, PTPRH, SLC4A4, MUC13, TFF1, HNF4G and TFF2) in the turquoise module were validated to be highly expressed in mEOC using two independent gene expression datasets GSE44104 and GSE30274. CONCLUSION: Our work proposed an applicable framework of molecular characteristics for patients with mEOC, which may help us to obtain a precise and comprehensive understanding on the molecular complexities of mEOC. The hub genes identified in our study, as potential specific biomarkers of mEOC, may be applied in the early diagnosis of mEOC in the future.


Assuntos
Carcinoma Epitelial do Ovário/genética , Cistadenoma Mucinoso/genética , Redes Reguladoras de Genes , Neoplasias Ovarianas/genética , Carcinoma Epitelial do Ovário/patologia , Estudos de Coortes , Cistadenoma Mucinoso/patologia , Feminino , Perfilação da Expressão Gênica/métodos , Regulação Neoplásica da Expressão Gênica , Humanos , Neoplasias Ovarianas/patologia , Transcriptoma
5.
J Am Chem Soc ; 141(16): 6651-6657, 2019 Apr 24.
Artigo em Inglês | MEDLINE | ID: mdl-30879294

RESUMO

The combustion has long been applied for industrial synthesis of carbon materials such as fullerenes as well as carbon particles (known as carbon black), but the components and structures of the carbon soot are far from being clarified. Herein, we retrieve an unprecedented hydrofullerene C66H4 from a soot of a low-pressure combustion of benzene-acetylene-oxygen. Unambiguously characterized by single-crystal X-ray diffraction, the C66H4 renders a nonclassical geometry incorporating two heptagons and two pairs of fused pentagons in a C2 v symmetry. The common vertexes of the fused pentagons are bonded with four hydrogen atoms to convert the hydrogen-linking carbon atoms from sp2 to sp3 hybridization, which together with the adjacent heptagons essentially releases the sp2-bond strains on the abutting-pentagon sites of the diheptagonal fused pentagon C66 (dihept-C66). DFT computations suggest the possibility for an in situ hydrogenation process leading to stabilization of the dihept-C66. In addition, the experiments have been carried out to study heptagon-dependent properties of dihept-C66H4, indicating the key responsibility of the heptagon for changing hydrocarbon activity and electronic properties. The present work with the unprecedented double-heptagon-containing hydrofullerene successfully isolated and identified as one of the low-pressure combustion products shows that the heptagon is a new building block for constructing fullerene products in addition to pentagons and hexagons in low-pressure combustion systems.

6.
Analyst ; 144(8): 2550-2555, 2019 Apr 08.
Artigo em Inglês | MEDLINE | ID: mdl-30865739

RESUMO

In this study, an ultrasensitive electrochemiluminescence (ECL) aptasensor based on Ru(bpy)32+-doped silica nanoparticles (Ru@SiO2 NPs) coupled with gold nanoparticles (Au NPs) was developed for the determination of ractopamine (Rac). TPrA is used as the coreactant, the Ru@SiO2 NPs serve as the ECL luminophores, and the Au NPs work as a catalyzer in the redox reaction as well as the carrier that immobilizes the aptamer. Meanwhile, the complete incorporation of the Ru@SiO2 NPs and Au NPs increases the localized surface plasmon resonance (LSPR) probability, thus promoting ECL emission. The ractopamine (Rac) target molecules are specifically captured on the electrode surface by aptamer recognition. The ECL signal is quenched by energy transfer from the luminophore to benzoquinone compounds, which are oxidative products of Rac from the electrochemical scanning process. The proposed ECL aptasensor exhibits ultrahigh sensitivity and excellent selectivity for Rac detection. The linear response ranged over Rac concentrations from 1.5 × 10-12 M to 1.5 × 10-8 M with a detection limit of 4.1 × 10-14 M (S/N = 3). The detection recovery of Rac in real meat samples confirmed the satisfactory performance of the method. This study describes a versatile ECL aptasensor based on the combined functions of luminous nanospheres and Au NPs, indicating its potential application for the ultrasensitive analysis of targets in diverse systems.

7.
Exp Neurol ; 311: 225-233, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30315808

RESUMO

ATP-sensitive potassium (K-ATP) channels, coupling cell metabolism to cell membrane potential, are involved in brain diseases including stroke. Emerging evidence shows that astrocytes play important roles in the pathophysiology of cerebral ischemia. Kir6.1, a pore-forming subunit of K-ATP channel, is prominently expressed in astrocytes and participates in regulating its function. However, the exact role of astrocytic Kir6.1-containg K-ATP channel (Kir6.1/K-ATP) in ischemic stroke remains unclear. Here, we found that astrocytic Kir6.1 knockout (KO) mice exhibited larger infarct areas and more severe brain edema and neurological deficits in middle cerebral artery occlusion stroke model. Both activated gliosis and neuronal loss were aggravated in astrocytic Kir6.1 KO mice. Furthermore, the protein levels of pro-apoptotic protein Bcl-2 associated X (Bax) and active caspase-3 were up-regulated and the expression of anti-apoptotic protein Bcl-2 was down-regulated in astrocytic Kir6.1 KO mice. This is accompanied by enhanced endoplasmic reticulum stress (ER stress) responses in brain tissues and in astrocytes during ischemia/reperfusion (I/R) injury. Finally, inhibition of ER stress rescued astrocyte apoptosis induced by Kir6.1 deletion during I/R injury. Collectively, our findings reveal that astrocytic Kir6.1/K-ATP channel protects brain from cerebral ischemia/reperfusion injury through inhibiting ER stress and suggest that astrocytic Kir6.1/K-ATP channel is a promising therapeutic target for ischemic stroke.


Assuntos
Astrócitos/metabolismo , Isquemia Encefálica/metabolismo , Estresse do Retículo Endoplasmático/fisiologia , Canais KATP/deficiência , Traumatismo por Reperfusão/metabolismo , Animais , Astrócitos/patologia , Isquemia Encefálica/genética , Isquemia Encefálica/patologia , Células Cultivadas , Canais KATP/genética , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Traumatismo por Reperfusão/genética , Traumatismo por Reperfusão/patologia
8.
Org Biomol Chem ; 16(34): 6284-6294, 2018 08 29.
Artigo em Inglês | MEDLINE | ID: mdl-30116808

RESUMO

An efficient palladium-catalyzed ortho-aroylation of O-arylmethyl and aryl-substituted acetoxime ethers has been developed; this method has high mono-site selectivity and does not require exogenous ligands. Under the direction of a simple exo-acetoxime auxiliary, a broad scope of masked arylmethyl alcohols and phenols as well as various aromatic aldehydes are compatible with this transformation, which probably follows a mechanistic pathway involving a six- or five-membered exo-cyclopalladated intermediate. The strategy can be expediently adopted to prepare synthetically valuable 1H-benzo[d][1,2]oxazines and benzo[d]isoxazoles. The directing group can be easily removed from the products to afford the functionalized diaryl ketones.

9.
J Diabetes Res ; 2018: 9216791, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29977927

RESUMO

Diabetic retinopathy is the leading cause of blindness, yet its treatment is very limited. Anti-VEGF drug has been widely applied in ocular disease, but its effects on diabetic retinopathy and the underlying mechanism have remained to be fully explored. To elucidate the role of anti-VEGF treatment, we sought to determine the effects of bevacizumab on diabetic neurovascular changes extending from the 3rd to 9th week with induced diabetes in adult rats. The retinal neurovascular changes included increased expression of VEGF, nNOS, iNOS, eNOS, and NO in the course of diabetes progression. In diabetic rats given bevacizumab injection, the ganglion cell loss and alterations of retinal thickness were ameliorated. In this connection, the immunofluorescence labeling of the above biomarkers was noticeably decreased. Along with this, Western blotting confirmed that bevacizumab treatment was associated with a decrease of VEGF, Flk-1, and cAMP response element binding and protein kinase C protein expression. The present results suggest that bevacizumab treatment in the early stage of the retinopathy may ameliorate the lesions of retinopathy, in which VEGF/Flk-1 signaling has been shown here to play an important role.


Assuntos
Inibidores da Angiogênese/uso terapêutico , Bevacizumab/uso terapêutico , Diabetes Mellitus Experimental/tratamento farmacológico , Retinopatia Diabética/tratamento farmacológico , Inibidores da Angiogênese/administração & dosagem , Animais , Bevacizumab/administração & dosagem , Diabetes Mellitus Experimental/metabolismo , Retinopatia Diabética/metabolismo , Injeções Intravítreas , Masculino , Ratos , Ratos Sprague-Dawley , Retina/efeitos dos fármacos , Retina/metabolismo , Fator A de Crescimento do Endotélio Vascular/metabolismo
10.
Anal Chem ; 90(8): 5075-5081, 2018 04 17.
Artigo em Inglês | MEDLINE | ID: mdl-29595051

RESUMO

Electrochemiluminescence (ECL) detection has attracted increasing attention as a promising analytical approach. A considerable number of studies showed that ECL intensity can be definitely improved by resonance energy transfer (RET), while the RET efficiency is strongly dependent on the distance between exited donors and acceptors. Herein we disclose for the first time a highly enhanced RET strategy to promote the energy transfer efficiency by coencapsulating the donor ([Ru(bpy)3]2+)/acceptor (CdTe quantum dots, CdTe QDs) pairs into a silica nanosphere. Plenty of [Ru(bpy)3]2+ and CdTe QDs closely packed inside a single nanosphere greatly shortens the electron-transfer path and increases the RET probability, therefore significantly enhancing the luminous efficiency. Further combining with molecularly imprinting technique, we develop a novel ECL sensor for ultrasensitive and highly selective detection of target molecules. Proof of concept experiments showed that extremely low detection limits of subfg/mL (S/N = 3) with broad linear ranges (fg/mL to ng/mL) could be obtained for detection of two kinds of mycotoxins (α-ergocryptine and ochratoxin A) that are recognized as potential health hazards at very low concentrations. This strategy combining enhanced RET system and molecularly imprinting technique, represents a versatile ECL platform toward low-cost, rapid, ultrasensitive, and highly selective detection of target molecules in diverse applications.

11.
J Pharmacol Exp Ther ; 364(3): 367-376, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-29298819

RESUMO

To investigate the role of histamine N-methyltransferase (HNMT) activity in the development of motion sickness (MS) in the dorsal vagal complex (DVC) to inform the development of new drugs for MS, Beagle dogs and Sprague-Dawley rats were rotated to simulate MS. HNMT expression in the brain stem and DVC was measured. The effects of systemic application of tacrine, an HNMT inhibitor, on the development of MS were observed. Moreover, we microinjected a histamine receptor H1 inhibitor, promethazine, into the DVC to verify the involvement of histaminergic neurotransmission in MS. Finally, lentiviral vectors were microinjected into the DVC to determine the effects of altered HNMT expression on MS. We found the following: 1) HNMT expression in the medulla oblongata of dogs and rats insusceptible to MS was higher than in susceptible animals; 2) tacrine dose-dependently promoted MS in both animals and raised histamine level in rat medulla oblongata; 3) blocking histaminergic neurotransmission in the DVC with promethazine inhibited MS; 4) rotatory stimulus induced an elevation in HNMT expression, and vestibular training elevated the basal level of HNMT in the DVC during habituation to MS; 5) in vivo transfection of a lentiviral vector packaged with the HNMT gene increased HNMT expression in the DVC and reduced MS; and 6) microinjection of a lentiviral vector driving the interference of HNMT gene expression in vivo significantly inhibited HNMT expression in the DVC and exacerbated MS. In conclusion, HNMT expression in the brain stem is inversely correlated with MS development. Increasing HNMT expression or stimulating its activity in the DVC could inhibit MS.


Assuntos
Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Histamina N-Metiltransferase/metabolismo , Terapia de Alvo Molecular , Enjoo devido ao Movimento/tratamento farmacológico , Enjoo devido ao Movimento/enzimologia , Nervo Vago/efeitos dos fármacos , Animais , Cães , Feminino , Histamina/metabolismo , Masculino , Enjoo devido ao Movimento/metabolismo , Ratos , Ratos Sprague-Dawley , Nervo Vago/metabolismo
12.
Angew Chem Int Ed Engl ; 56(32): 9454-9458, 2017 08 01.
Artigo em Inglês | MEDLINE | ID: mdl-28636246

RESUMO

Existing methods offer little direct and real-time information about stretch-triggered biochemical responses during cell mechanotransduction. A novel stretchable electrochemical sensor is reported that takes advantage of a hierarchical percolation network of carbon nanotubes and gold nanotubes (CNT-AuNT). This hybrid nanostructure provides the sensor with excellent time-reproducible mechanical and electrochemical performances while granting very good cellular compatibility, making it perfectly apt to induce and monitor simultaneously transient biochemical signals. This is validated by monitoring stretch-induced transient release of small signaling molecules by both endothelial and epithelial cells cultured on this sensor and submitted to stretching strains of different intensities. This work demonstrates that the hybrid CNT-AuNT platform offers a versatile and highly sensitive way to characterize and quantify short-time mechanotransduction responses.


Assuntos
Técnicas Biossensoriais , Técnicas Eletroquímicas , Ouro/química , Células Endoteliais da Veia Umbilical Humana/química , Mecanotransdução Celular , Nanopartículas Metálicas/química , Nanotubos de Carbono/química , Células Cultivadas , Células Endoteliais da Veia Umbilical Humana/metabolismo , Humanos , Óxido Nítrico/biossíntese , Óxido Nítrico/química , Óxido Nítrico Sintase Tipo III/metabolismo , Tamanho da Partícula , Fatores de Tempo
14.
Diving Hyperb Med ; 47(1): 17-23, 2017 03.
Artigo em Inglês | MEDLINE | ID: mdl-28357820

RESUMO

BACKGROUND AND AIMS: Severe decompression sickness (DCS) is a multi-organ injury. This study investigated the preventive effects of rosiglitazone on liver injury following rapid decompression in mice and examined the underlying mechanisms. METHODS: Mice were randomly divided into four groups: a control group, vehicle group, and rosiglitazone (5 and 10 mg·kg⁻¹) groups, the latter three being exposed to a pressure of 911 kPa. Haematoxylin and eosin staining, plasma levels of alanine transaminase (ALT), aspartate transaminase (AST) and lactate dehydrogenase and blood cell counts were used to evaluate liver injury at 30 min after rapid decompression. The expression of endothelial and inducible nitric oxide synthase (iNOS) and its phosphorylation were measured to uncover the underlying molecular mechanisms. RESULTS: A significant increase in plasma ALT, red blood cells and platelets, and a decrease in neutrophils were observed in the vehicle group. Furthermore, the expression of iNOS, E-selectin and the total level of NO in hepatic tissue, and soluble E-selectin in the plasma were significantly elevated in the vehicle group. Rosiglitazone pre-treatment prevented the increases in ALT (and AST), soluble E-selectin concentration, red blood cells and platelet counts. Moreover, rosiglitazone reduced over-expression of iNOS and the NO level, prevented the fall in neutrophil count and promoted the phosphorylation of iNOS in the liver. CONCLUSIONS: Pre-treatment with rosiglitazone ameliorated liver injury from severe DCS. This preventive effect may be partly mediated by stimulating endothelial NO production, improving endothelial function and limiting inflammatory processes.


Assuntos
Doença da Descompressão/complicações , Hipoglicemiantes/farmacologia , Fígado/efeitos dos fármacos , Fígado/lesões , Tiazolidinedionas/farmacologia , Alanina Transaminase/sangue , Animais , Aspartato Aminotransferases/sangue , Doença da Descompressão/sangue , Doença da Descompressão/metabolismo , Modelos Animais de Doenças , Selectina E/metabolismo , Contagem de Eritrócitos , L-Lactato Desidrogenase/sangue , Contagem de Leucócitos , Fígado/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos ICR/sangue , Camundongos Endogâmicos ICR/metabolismo , Neutrófilos/efeitos dos fármacos , Óxido Nítrico/metabolismo , Óxido Nítrico Sintase Tipo II/metabolismo , Óxido Nítrico Sintase Tipo III/metabolismo , Fosforilação/efeitos dos fármacos , Contagem de Plaquetas , Distribuição Aleatória , Rosiglitazona
15.
Chemosphere ; 174: 774-780, 2017 05.
Artigo em Inglês | MEDLINE | ID: mdl-28196686

RESUMO

The present study used strain ZH-H2 (Fusarium sp.) isolated by our group as the PAH-degrading strain and 5-6-rings PAHs as degradation objects. The soil incubation experiment was carried out to investigate the starch-enhanced degradation effects of HMW PAHs by Fusarium sp. in an Aged Polluted Soil from a Coal Mining Area. The results showed that the removal rates of BaP, InP and BghiP increased with increasing inoculation rate of ZH-H2 in the unsterile aged polluted soil of coal mining area, with the exception of BbF degradation which increased in the H2 treatment and then decreased. Different addition dosage of starch apparently resulted in degradation of 4 PAHs in soil, with removal rates of 14.47% for BaP, 23.83% for DbA, 30.77% for BghiP and 31.00% for InP obtained with treatment D2, respectively higher than in treatment D1. So starch addition apparently enhanced the degradation of the 4 PAHs, especially InP and BghiP, by native microbes in the aged HMW PAH-polluted soil. By adding starch to these aged polluted soils with inoculated strain ZH-H2, HMW-PAHs degradation was further improved and addition of 0.5 g kg-1 starch to soils with 1.0 g kg-1 Fusarium ZH-H2 (D2 + H2) performed best to the 4 HMW-PAHs in all of these combination treatments by a factor of up to 3.09, depending on the PAH. We found that the highest polyphenol oxidase activities under D2 + H2 treatments are consistent with the results of removal rates of 4 PAHs. Our findings suggest that the combination of Fusarium sp. ZH-H2 and starch offers a suitable alternative for bioremediation of aged PAH-contaminated soil in coal mining areas, with a recommended inoculation size of 0.5 g Fusarium sp. ZH-H2 and addition of 0.5 g kg-1 starch per kg soil.


Assuntos
Minas de Carvão , Fusarium/efeitos dos fármacos , Fusarium/metabolismo , Hidrocarbonetos Policíclicos Aromáticos/química , Hidrocarbonetos Policíclicos Aromáticos/metabolismo , Amido/farmacologia , Biodegradação Ambiental/efeitos dos fármacos , Peso Molecular , Solo/química , Poluentes do Solo/química , Poluentes do Solo/metabolismo , Fatores de Tempo
16.
Carbohydr Polym ; 152: 241-252, 2016 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-27516270

RESUMO

A homogenous water-soluble polysaccharide, DPSW-A, with a deduced chemical structure was extracted from the herb Taraxacum mongolicum Hand.-Mazz. Moreover, 80.813-kDa DPSW-A is composed of three types of monosaccharide, namely rhamnose, arabinose, and galactose, at a molar ratio of 1.0:10.7:11.9. The main chain of DPSW-A contains Terminal-Galp, 1,3-Galp, 1,6-Galp, 1,3,6-Galp, and 1,2,4-Rhap; the branched chain contains Terminal-Araf, 1,5-Araf, and 1,3,5-Araf. The sulfated derivatives prepared from DPSW-A showed inhibitory effects on complement activation through the classical pathway (CH50: Sul-DPSW-A, 3.94±0.43µg/mL; heparin, 104.40±3.82µg/mL) and alternative pathway (AP50: Sul-DPSW-A, 42.76±0.46µg/mL; heparin, 43.42±0.22µg/mL). Mechanism studies indicated that Sul-DPSW-A inhibited complement activation by blocking C1q, C1r, C1s, and C9, but not C2, C3, C4, and C5. In addition, Sul-DPSW-A displayed limited anticoagulant effects. These results suggest that Sul-DPSW-A prepared from DPSW-A is valuable for treating diseases caused by excessive complement system activation.


Assuntos
Ativação do Complemento/efeitos dos fármacos , Proteínas Inativadoras do Complemento/farmacologia , Polissacarídeos/química , Polissacarídeos/farmacologia , Sulfatos , Taraxacum/química , Animais , Proteínas Inativadoras do Complemento/química , Proteínas Inativadoras do Complemento/isolamento & purificação , Eritrócitos/efeitos dos fármacos , Eritrócitos/metabolismo , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Polissacarídeos/análise , Polissacarídeos/isolamento & purificação , Coelhos , Ovinos , Sulfatos/análise , Sulfatos/química , Sulfatos/isolamento & purificação , Sulfatos/farmacologia
17.
J Am Chem Soc ; 138(30): 9629-33, 2016 08 03.
Artigo em Inglês | MEDLINE | ID: mdl-27377559

RESUMO

Curvature prevalently exists in the world of carbon materials (e.g., fullerenes, buckyl bowls, carbon nanotubes, and onions), but traditional C2-addition mechanisms fail to elucidate the mechanism responsible for the formation of carbon curvature starting from a pentagonal carbon ring in currently available chemical-physical processes such as combustion. Here, we show a complete series of nascent pentagon-incorporating C5-C18 that are online produced in the flame of acetylene-cyclopentadiene-oxygen and in situ captured by C60 or trapped as polycyclic aromatic hydrocarbons for clarifying the growth of the curved subunit of C20H10. A mechanism regarding C1-substitution and C2-addition has been proposed for understanding the formation of curvature in carbon materials, as exemplified by the typical curved molecule containing a single pentagon completely surrounded by five hexagons. The present mechanism, supported by the intermediates characterized by X-ray crystallography as well as NMR, has been experimentally validated for the rational synthesis of curved molecule in the commercially useful combustion process.

18.
Zhongguo Zhong Xi Yi Jie He Za Zhi ; 36(8): 929-932, 2016 08.
Artigo em Chinês | MEDLINE | ID: mdl-30640986

RESUMO

Objective To observe distribution laws and features of syndrome types of Chinese medicine (CM) in hyperlipidemia patients of Han, Uyghur, Kazakh nationalities in Xinjiang Uyghur Auton- omous Region. Methods Using cluster random sampling, 1 410 hyperlipidemia patients (18 -70 years old ) were recruited from Urumqi, Turpan, Altay, Ili, Aksu, Hetian in Xinjiang Uyghur Autonomous Re- gion. The general condition, susceptible factors, classification of blood lipids, complications, syndromes of CM, tongue figure, etc. clinical data were investigated using self-formulated Epidemiological Investiga- tion Questionnaire on Susceptible Factors in Different Nationalities of Hyperlipemia Patients in Xinjiang (abbreviated as Questionnaire thereafter). Factor analysis and cluster analysis were performed. Results Cronbach's coefficient for the 54 syndrome items in Questionnaire was 0.891, Kaiser-Meyer-Olkin (KMO) 0. 897, Sig <0.05 in Bartlett's sphericity test. Seventeen common factors were obtained using principal component analysis (PCA). Totally 54 common symptoms of hyperlipidemia were screened, which were then divided into 17 groups with 1 -6 symptoms in each group. F4 (soreness and weakness of waist and knees, sour pain in joints and muscles, body numbness, heavy body sensation, cold limbs), F5 (frequent and clear nocturia, dysuria,-dribble of urine, frequent urination at night), F10 (thirsty, no desire for water, tastelessness, hydroadipsia) , F12 (a white complexion with puffiness, hid- ing fever, hypoactive sexual desire) , and F17 (enuresis) were merged as Shen yang deficiency (SYD) ; F2 (fatigue, drowsiness, depression, spiritlessness, fatigue and disinclination to talk) and F15 (poor ap- petite) were merged as Pi-qi deficiency (PQD) ; F3 (dry mouth and dry pharynx, thirsty, propensity for water, bitter mouth, greasy mouth, stingy mouth, irritability and upset) and F16 (dark red tongue proper, greasy tongue fur) were merged as damp-heat trapped in Pi (DHTP). Results of cluster analysis showed that Pi-Shen deficiency (PSD) was most often seen in hyperlipidemia, and main syndrome types were sequenced from high to low as Pi-Shen deficiency type (46. 2%, 652/1 410) , blockage of cardiac vessels type ( 31. 1% , 438/1 410 ), phlegm and blood stasis internal resistance type ( 13. 3% , 187/1 410), Pi-deficiency induced damp abundance type (8. 3%, 123/1 410), Gan-Shen yin deficiency type (0. 7%, 10/1 410). Conclusions Deficiency syndrome was dominant in hyperlipidemia patients of Xinjiang Uyghur Autonomous Region. Phlegm turbidity, damp heat, and etc. were often complicated. The complex situation was manifested to be involved in multiple organs, qi-blood-fluid mixed disease.


Assuntos
Hiperlipidemias , Medicina Tradicional Chinesa , Adolescente , Adulto , Idoso , China , Humanos , Hiperlipidemias/diagnóstico , Hiperlipidemias/terapia , Pessoa de Meia-Idade , Síndrome , Deficiência da Energia Yang , Deficiência da Energia Yin , Adulto Jovem
19.
Asian J Androl ; 17(6): 942-7, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25851655

RESUMO

Peroxisome proliferator-activated receptor gamma (PPARγ) is a member of the PPARs, which are transcription factors of the steroid receptor superfamily. PPARγ acts as an important molecule for regulating energy homeostasis, modulates the hypothalamic-pituitary-gonadal (HPG) axis, and is reciprocally regulated by HPG. In the human, PPARγ protein is highly expressed in ejaculated spermatozoa, implying a possible role of PPARγ signaling in regulating sperm energy dissipation. PPARγ protein is also expressed in Sertoli cells and germ cells (spermatocytes). Its activation can be induced during capacitation and the acrosome reaction. This mini-review will focus on how PPARγ signaling may affect fertility and sperm quality and the potential reversibility of these adverse effects.


Assuntos
Reação Acrossômica/fisiologia , Metabolismo Energético/fisiologia , Fertilidade/fisiologia , PPAR gama/metabolismo , Células de Sertoli/metabolismo , Capacitação Espermática/fisiologia , Espermatócitos/metabolismo , Espermatozoides/metabolismo , Animais , Fertilização/fisiologia , Humanos , Sistema Hipotálamo-Hipofisário/metabolismo , Infertilidade Masculina/metabolismo , Leptina/metabolismo , Masculino , PPAR gama/fisiologia , Fosfatidilinositol 3-Quinases/metabolismo , Análise do Sêmen , Transdução de Sinais , Espermatogênese/fisiologia , Espermatozoides/fisiologia , Testículo/metabolismo
20.
J Ethnopharmacol ; 165: 9-19, 2015 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-25704929

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: "Shengyu" decoction, a traditional Chinese medicine, has been used to treat diseases with deficit in "qi" and "blood". The modified "Shengyu" decoction (MSD) used in the present study was designed to treat traumatic brain injury (TBI) on the basis of the "Shengyu" decoction, in which additional four herbs were added. Many ingredients in these herbs have been demonstrated to be effective for the treatment of brain injury. The present study was performed to evaluate the neurorestorative effect and the underlying mechanisms of MSD on the rat brain after a TBI. MATERIALS AND METHODS: TBI was induced in the right cerebral cortex of adult rats using Feeney's weight-drop method. Intragastrical administration of MSD (1.0 ml/200 g) was begun 6h after TBI. The neurological functions and neuronal loss in the cortex and hippocampus were determined. The levels of nerve growth-related factors GDNF, NGF, NCAM, TN-C, and Nogo-A and the number of GFAP(+)/GDNF(+), BrdU(+)/nestin(+), BrdU(+)/NeuN(+) immunoreactive cells in the brain ipsilateral to TBI were also measured. Moreover, the influences of MSD on these variables were observed at the same time. RESULTS: We found that treatment with MSD in TBI rats ameliorated the neurological functions and alleviated neuronal loss. MSD treatment elevated the expression of GDNF, NGF, NCAM, and TN-C, and inhibited the expression of Nogo-A. Moreover, MSD treatment increased the number of GFAP(+)/GDNF(+), BrdU(+)/nestin(+), and BrdU(+)/NeuN(+) immunoreactive cells in the cortex and hippocampus. CONCLUSION: The present results suggest that MSD treatment in TBI rats could improve the proliferation of neural stem/progenitor cells and differentiation into neurons, which may facilitate neural regeneration and tissue repair and thus contribute to the recovery of neurological functions. These effects of modified "Shengyu" decoction may provide a foundation for the use of MSD as a prescription of medicinal herbs in the traditional medicine to treat brain injuries in order to improve the neurorestoration.


Assuntos
Lesões Encefálicas/tratamento farmacológico , Proliferação de Células/efeitos dos fármacos , Medicamentos de Ervas Chinesas/farmacologia , Medicina Tradicional Chinesa/métodos , Células-Tronco Neurais/efeitos dos fármacos , Animais , Córtex Cerebral/citologia , Córtex Cerebral/efeitos dos fármacos , Córtex Cerebral/lesões , Hipocampo/citologia , Hipocampo/efeitos dos fármacos , Masculino , Regeneração Nervosa/efeitos dos fármacos , Células-Tronco Neurais/fisiologia , Ratos , Ratos Sprague-Dawley
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