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1.
Nat Prod Res ; : 1-10, 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-32998585

RESUMO

Strain SZW01 was isolated from sea sediment collected from Shenzhen in Guangdong province, China, and was later identified as Aspergillus fumigatus by16S rDNA sequence analysis. Various chromatographic processes led to the isolation and purification of three compounds from the fermentation culture of SZW01, including a new compound, 2,6'-dihydroxy-2,4'dimethoxy-8'-methyl-6-methoxy-acyl-ethyl-diphenylmethanone (1), and two known compounds: fumigaclavine C (2) and alternarosin A (3), as characterised by UV, IR, 1 D, 2 D-NMR and MS data. The antioxidant and α-glucosidase inhibitory activities of these compounds were evaluated. The result illustrated that compound 1 exhibited a moderate antioxidant activity and stronger α-glucosidase inhibitory activity than acarbose.

2.
Sci Adv ; 6(39)2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32978155

RESUMO

Biocrusts cover ~30% of global drylands with a prominent role in the biogeochemical cycles. Theoretically, biocrusts, vascular plants, and bare soil can represent multiple stable states in drylands. However, no empirical evidence for the existence of a biocrust stable state has been reported. Here, using a global drylands dataset, we found that biocrusts form an alternative stable state (biocrust cover, ~80%; vascular cover, ≤10%) besides bare soil (both biocrust and vascular cover, ≤10%) and vascular plants (vascular cover, >50%; biocrust cover, ~5%). The pattern of multiple stable states associated with biocrusts differs from the classic fold bifurcation, and values of the aridity index in the range of 0 to 0.6 define a bistable region where multiple stable states coexist. This study empirically demonstrates the existence and thresholds of multiple stable states associated with biocrusts along climatic gradients and thus may greatly contribute to conservation and restoration of global drylands.

3.
J Pediatr Urol ; 2020 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-32919900

RESUMO

OBJECTIVE: To assess the efficacy and safety of various minimally invasive procedures for 10-20 mm pediatric renal stones by Bayesian network meta-analysis (NWA). METHODS: We searched clinical comparative trials published in Pubmed, Embase, Cochrane Library from inception to 12 April 2020. Two researchers evaluated the quality and extracted data individually. Data was analyzed using STATA and GEMYC R package. RESULTS: The overall network meta-analytic outcome of stone free rate (SFR) in a single session revealed that Retrograde Intrarenal Stone Surgery (RIRS), miniaturized percutaneous nephrolithotomy (mPCNL) and PCNL showed superiority to extracorporeal shockwave lithotripsy (ESWL). Statistical significance was not detected between any intervention from our pooled network analysis of complication rate. SMP was the most likely to ranking in first place to render stone free status, and it also showed the lowest risk possibility of complications. Mini-PCNL had longer operation time and hospitalization than ESWL. The global and loop inconsistency evaluation demonstrated a rather acceptable outcome apart from the comparisons of complication rate between two randomized control studies. DISCUSSION: Herein, the authors reviewed and explored the optimal management pattern for pediatric 10-20 mm renal stones. This NWA revealed RIRS and mPCNL could render higher SFR without increasing risk of complications compared with ESWL. Although SMP was deemed to be the best choice in our study, the limited source of study and sample size implied the results required to be further validated. In addition, there were still some problems requiring to be underlined for various surgical options. CONCLUSIONS: ESWL was inferior to RIRS, mPCNL and PCNL for 10-20 mm pediatric renal stones, among which SMP might be the most ideal option associated with the least possibility of complications and the highest probability of stone clearance.

4.
Rev Neurosci ; 2020 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-32931450

RESUMO

The benefit of endovascular treatment (EVT) for patients with intracranial atherosclerosis-related large vessel occlusion (ICAS-LVO) in posterior circulation stroke (PCS) is inconsistent. This systematic review and meta-analysis were conducted to estimate the effect of ICAS-LVO in PCS treated by EVT. A systematic review was completed, tracking studies from their date of inception until February 2020. Clinical studies which compared outcomes after EVT for ICAS-LVO and non-ICAS-LVO in PCS were included. Data were synthesized and interpreted from meta-analysis. A total of 688 patients (352 ICAS-LVO and 336 non-ICAS-LVO) in the eight studies were included. The successful reperfusion rate (odds ratio [OR], 0.58; 95% confidence intervals [95% CIs], 0.37-0.93; P = 0.02) was lower in PCS with ICAS-LVO than non-ICAS-LVO. And for other clinical outcomes, there were no differences between both groups. Moreover, there were no statistical differences of any clinical outcome among subgroups stratified by nations and target vessel occlusion location. With respect to patients' characteristics, age (mean difference [MD], -2.75; 95% CI, -4.62--0.88; P = 0.004), pc-Alberta Stroke Program Early CT Score (MD, -0.49; 95% CI, -0.94--0.05; P = 0.03), distributions of sex (male) (OR, 2.34; 95% CI, 1.53-3.56; P < 0.001), prior or current smoking (OR, 1.85; 95% CI, 1.12-3.07; P = 0.02), hypertension (OR, 2.06; 95% CI, 1.32-3.22; P = 0.002), coronary artery disease (OR, 0.27; 95% CI, 0.11-0.66; P = 0.004) and general anesthesia (OR, 2.89; 95% CI, 1.54-5.45; P = 0.001) were statistically different between both groups. In conclusion, more targeted assessments are warranted for patients with ICAS-LVO-related PCS during clinical strategies, and the benefit of EVT for PCS with ICAS-LVO deserves further research.

5.
Bioresour Technol ; 318: 124064, 2020 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-32905949

RESUMO

One challenge in metabolic engineering for industrial applications is the construction of highly efficient microbial cell factories. For this purpose, dynamic regulation of metabolic flux may be indispensable. In this study, an auto-regulated Corynebacterium glutamicum chassis for 5-aminolevulinic acid (5-ALA) biosynthesis was constructed. First, the expression of critical genes involved in 5-ALA synthesis and cofactor regeneration was precisely modulated. Furthermore, odhA expression was controlled using the strategies of static metabolic engineering (SME, with a weak promoter), dynamic metabolic engineering (DME, with a temperature-sensitive plasmid), and auto-inducible metabolic engineering (AME, with a growth-related promoter). The AME strategy showed the best effect and dynamically balanced the tradeoff between cell growth and 5-ALA synthesis. Additionally, the expression of exporter-encoding rhtA was regulated using AME strategy by the two-component system HrrSA in response to extracellular heme. The final strain A30 achieved the highest 5-ALA production (3.16 g/L) ever reported in C. glutamicum through C5 pathway.

6.
Acta Crystallogr C Struct Chem ; 76(Pt 9): 850-855, 2020 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-32887854

RESUMO

A novel twofold interpenetrating two-dimensional (2D) ZnII coordination framework, poly[[(µ-1,3-bis(2-methyl-1H-imidazol-1-yl)benzene-κ2N3:N3)(µ-naphthalene-2,6-dicarboxylato-κ2O2:O6)zinc(II)] dimethylformamide monosolvate], {[Zn(C12H6O4)(C14H14N4)]·C3H7NO}n or {[Zn(1,3-BMIB)(NDC)]·DMF}n (I), where H2NDC is naphthalene-2,6-dicarboxylic acid, 1,3-BMIB is 1,3-bis(2-methyl-1H-imidazol-1-yl)benzene and DMF is dimethylformamide, was prepared and characterized through IR spectroscopy, elemental analysis, thermal analysis and single-crystal X-ray diffraction. Single-crystal X-ray diffraction analysis revealed that (I) exhibits an unusual twofold interpenetrating 2D network. In addition, it displays strong fluorescence emissions and a high photocatalytic activity for the degradation of Rhodamine B (RhB) under UV-light irradiation.

7.
Medicine (Baltimore) ; 99(33): e21706, 2020 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-32872046

RESUMO

MicroRNAs (miRNAs) have been suggested to act critical roles in the pathophysiology of traumatic osteonecrosis of the femoral head (TONFH). Unfortunately, their roles in the development of TONFH are still ambiguous. The purpose of this study is to identify promising miRNA biomarkers in traumatic osteonecrosis development.We conducted a comprehensive bioinformatics analysis using microarray datasets downloaded from the Gene Expression Omnibus database, and compared the expression of miRNAs in the serum of TONFH patients with controls. Next, we performed target prediction, function enrichment analysis, and protein-protein interaction network analysis based on differentially expressed (DE) miRNAs.We identified 26 DE miRNAs that may contribute to the pathophysiology of TONFH. The miRNAs were linked to ubiquitin proteasome system including conjugating protein ligase activity, ubiquitin-protein ligase activity and ubiquitin mediated proteolysis 5 pathway, and we exposed miR-181a-5p and miR-140-5p as promising biomarkers in TONFH.A predicting model consisting of 5 miRNAs may help discriminating high-risk patients who might develop TONFH after femur neck fracture. Among DE miRNAs, MiR-181a-5p and miR-140-5p may contribute to the development femoral head osteonecrosis after femur neck fracture via ubiquitin proteasome system.


Assuntos
Fraturas do Colo Femoral/genética , Necrose da Cabeça do Fêmur/genética , MicroRNAs/análise , Ubiquitina/genética , Biomarcadores/metabolismo , Biologia Computacional , Feminino , Fraturas do Colo Femoral/cirurgia , Perfilação da Expressão Gênica , Humanos , Masculino , MicroRNAs/genética
8.
Protoplasma ; 2020 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-32929630

RESUMO

Wheat contains the largest number of miR396 family with 17 miR396 in Poaceae. MiR396 regulatory network underlying wheat grain development has not comprehensively been explored. Our results showed that precursor miR396 family in Poaceae exhibited not only conservativeness but also diversification especially in wheat. Five haplotypes were detected in Poaceae species, while 4 haplotypes in wheat with Hap-4 (miR396a) and Hap-5 (miR396n) unique to wheat. GO enrichment analysis of target genes showed that the first 20 enrichment functions of miR396a and miR396n are completely different from each other, and also completely different from miR396(b-g), miR396(h-m), and miR396(o-q). Functional annotation on the 18 target genes shared by miR396(b-g), miR396(h-m), and miR396(o-q) found that 11 of the 18 target genes are growth-regulating factor (GRF) genes. Our results indicated that, during the grain filling stage of wheat, miR396 is involved in the development of grains by regulating the expression of GRF genes (GRF1, GRF6, and GRF9). Although the enrichment function of miR396(b-g), miR396(h-m), and miR396(o-q) is the same, the gene functional networks they formed differ greatly. Our results indicated that polyploidization enriches not only the diversity of miR396 family and its target genes but also gene functional networks in wheat. These results laid foundation for further elucidating function of miR396 gene family underlying wheat grain development.

9.
Restor Neurol Neurosci ; 38(4): 311-321, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32925118

RESUMO

BACKGROUND: Statin therapy has been shown to be effective in the prevention of ischemic stroke. In addition, recent studies have suggested that prior statin therapy could lower the initial stroke severity and improve stroke functional outcomes in the event of stroke. It was speculated that prestroke statin use may enhance collateral circulation and result in favorable functional outcomes. OBJECTIVE: The aim of the study was to investigate the association of prestroke statin use with leptomeningeal collaterals and to determine the association of prestroke statin use with stroke severity and functional outcome in acute ischemic stroke patients. METHODS: We prospectively and consecutively enrolled 239 acute ischemic stroke patients with acute infarction due to occlusion of the middle cerebral artery within 24 h in the neurology department of West China Hospital from May 2011 to April 2017. Computed tomographic angiography (CTA) imaging was performed for all patients to detect middle cerebral artery thrombus; regional leptomeningeal collateral score (rLMCS) was used to assess the degree of collateral circulation; the National Institutes of Health Stroke Scale (NIHSS) was used to measure stroke severity at admission; the modified Rankin scale (mRS) was used to measure outcome at 90 days; and premorbid medications were recorded. Univariate and multivariate analyses were performed. RESULTS: Overall, 239 patients met the inclusion criteria. Fifty-four patients used statins, and 185 did not use statins before stroke onset. Prestroke statin use was independently associated with good collateral circulation (rLMCS > 10) (odds ratio [OR], 4.786; 95% confidence interval [CI], 1.195-19.171; P = 0.027). Prestroke statin use was not independently associated with lower stroke severity (NIHSS score≤14) (OR, 1.955; 95% CI, 0.657-5.816; p = 0.228), but prestroke statin use was independently associated with favorable outcome (mRS score≤2) (OR, 3.868; 95% CI, 1.325-11.289; P = 0.013). CONCLUSIONS: Our findings suggest that prestroke statin use was associated with good leptomeningeal collaterals and clinical outcomes in acute ischemic stroke (AIS) patients presenting with occlusion of the middle cerebral artery. However, clinical studies should be conducted to verify this claim.

10.
BMC Genomics ; 21(1): 674, 2020 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-32993537

RESUMO

BACKGROUND: Fuzhong buffalo, a native breed of Guangxi Zhuang Autonomous Region, is traditionally used as a draft animal to provide farm power in the rice cultivation. In addition, the Fuzhong buffalo also prepared for the bullfighting festival organized by the locals. The detection of the selective signatures in its genome can help in elucidating the selection mechanisms in its stamina and muscle development of a draft animal. RESULTS: In this study, we analyzed 27 whole genomes of buffalo (including 15 Fuzhong buffalo genomes and 12 published buffalo genomes from Upper Yangtze region). The ZHp, ZFst, π-Ratio, and XP-EHH statistics were used to identify the candidate signatures of positive selection in Fuzhong buffalo. Our results detected a set of candidate genes involving in the pathways and GO terms associated with the response to exercise (e.g., ALDOA, STAT3, AKT2, EIF4E2, CACNA2D2, TCF4, CDH2), immunity (e.g., PTPN22, NKX2-3, PIK3R1, ITK, TMEM173), nervous system (e.g., PTPN21, ROBO1, HOMER1, MAGI2, SLC1A3, NRG3, SNAP47, CTNNA2, ADGRL3). In addition, we also identified several genes related to production and growth traits (e.g., PHLPP1, PRKN, MACF1, UCN3, RALGAPA1, PHKB, PKD1L). Our results depicted several pathways, GO terms, and candidate genes to be associated with response to exercise, immunity, nervous system, and growth traits. CONCLUSIONS: The selective sweep analysis of the Fuzhong buffalo demonstrated positive selection pressure on potential target genes involved in behavior, immunity, and growth traits, etc. Our findings provided a valuable resource for future research on buffalo breeding and an insight into the mechanisms of artificial selection.

11.
BMJ Open ; 10(9): e038173, 2020 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-32994246

RESUMO

OBJECTIVES: Since 2010, the Chinese government has gradually increased its investment in the training of general practitioners (GPs) to support their role as 'gatekeepers' in the healthcare system. However, this training is still organised from the perspective of specialist care. We aimed to assess the appropriateness of the principal GP admission training programme curricula in China, including Residents Training for GPs (RTGP), Residents Training for Assistant GPs (RTAGP) and Training for Specialists with General Practice interest (TSGP). SETTING: The study focussed on GP training programmes in Shanghai, China. PARTICIPANTS: Data on disease competences developed in three GP clinical training programmes (RTGP, RTAGP and TSGP) were derived from official programme training manuals. Data on the proportion of outpatient visits for each disease were taken from the Shanghai community healthcare centres grassroots outpatient database. PRIMARY AND SECONDARY OUTCOME MEASURES: We first conducted a quantitative analysis by comparing the structure of current training curricula with actual outpatient utilisation patterns across all community healthcare institutions in Shanghai from 2014 to 2018. Qualitative analysis was then conducted to evaluate GP training programmes based on Donabedian's model. RESULTS: Quantitative analysis showed that the distribution of diseases for which competences were taught did not match the composition of outpatient visits in community healthcare institutions. Concerns identified through qualitative analysis included teachers who were mostly specialists, lack of equipment for differential diagnosis in community healthcare institutions, insufficient teaching of referral standards and GP training in large hospitals that consistently neglected communication skills. CONCLUSIONS: The establishment and implementation of admission training programmes for GPs in China lacks a focus on general practice training and may be improved by adopting an evidence-based general perspective aligned with the medical needs of the community.

12.
J Exp Bot ; 2020 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-32936902

RESUMO

In plants, 3', 5'-cyclic adenosine monophosphate (cAMP) has been recognized as an important second messenger with varied functions. However, only a few adenylyl cyclases (ACs) synthesizing cAMP have been identified. Moreover, the biological roles of ACs/cAMP in response to stress remain largely unclear. Here, using the quantitative proteomics techniques, we identified a heat-induced putative disease resistance RPP13-like protein 3 (ZmRPP13-LK3) with three conserved catalytic AC centres. Subsequently, the AC activity of ZmRPP13-LK3 was confirmed by in vitro enzyme activity analysis, in vivo RNAi experiments and functional complementation in E. coli cyaA mutant. ZmRPP13-LK3 is located in the mitochondrion. In vitro and vivo experimental results indicated ZmRPP13-LK3 interacted with ZmABC2, a possible cAMP exporter. Under heat stress, the levels of ZmRPP13-LK3 and cAMP in ABA-deficient mutant vp5 were prominently less than those in wild-type Vp5; In Vp5, ABA and its inhibitor treatment affected ZmRPP13-LK3 expression. The application of 8-Br-cAMP, a cAMP analogue increased heat-induced HSPs expression and alleviated heat-activated oxidative stress in maize. Taken together, our results showed that ZmRPP13-LK3, a new AC, could catalyse ATP for producing cAMP and may be involved in ABA-regulated heat resistance. Thus, this study could enrich our knowledge about plant response to heat stress.

13.
J Genet ; 992020.
Artigo em Inglês | MEDLINE | ID: mdl-32893840

RESUMO

Members of the bZIP transcription factor family play crucial roles in the regulation of plant development, biosynthesis of secondary metabolites, and response to abiotic and biotic stresses. To date, multiple bZIPs have been identified and investigated in numerous plant species. However, few studies have characterized bZIPs from Dimocarpus longan Lour. In this study, nine bZIPs from D. longan were identified from RNA-Seq data and further verified using the NCBI conserved domain search tool and Pfam database. Bioinformatics tools were used to systematically analyse the physicochemical properties, protein structures, multiple sequence alignment, motif compositions, evolutionary relationships, secondary structures, subcellular localization, phosphorylation sites, signal peptides, GO annotations and protein-protein interactions of the DlbZIPs. The expression patterns of the nine DlbZIPs were evaluated by qRT-PCR in roots and leaves and in response to varying durations of a 38°C heat treatment. DlbZIP3, DlbZIP5, DlbZIP6 and DlbZIP7 were differentially expressed between root and leaf tissues. All nine DlbZIPs responded to heat treatment in both roots and leaves, but their specific expression levels differed. DlbZIP4 and DlbZIP8 were highly expressed in roots after heat treatment, whereas DlbZIP1 and DlbZIP5 were highly expressed in leaves after heat treatment. These findings lay a foundation for increasing active secondary metabolite content and improving abiotic stress tolerance in D. longan using transgenic technology.

14.
Environ Monit Assess ; 192(10): 651, 2020 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-32964290

RESUMO

Cadmium (Cd) pollution in plastic shed soils has become increasingly severe, posing a great threat to human health and social stability. Phytoremediation of cadmium pollution is an environmentally friendly and inexpensive remediation method. In this study, maize (Zea mays L.) was selected as the phytoremediation crop by a potted method, and the bioavailability of cadmium was investigated by adding exogenous elemental sulfur. The relationships among the sulfur content, maize growth, cadmium accumulation, and soil parameters were systematically studied. The results showed that, with the supplement of sulfur, the soil pH and activities of soil enzymes (urease, catalase, and sucrase) decreased gradually, and the available heavy metals (Cd, Cr, Zn, and Cu) in soil showed an upward trend. The optimal cadmium enrichment was achieved under T2 by increasing both the biomass of the maize plant and the cadmium concentration in roots and stems. However, T3 and T4 significantly inhibited the growth of maize roots and shoots, leading to a much lower plant biomass compared with that of CK (sulfur-free treatment) and T2. In addition, the cumulative cadmium was not increased because of the low accumulation of cadmium in some parts of the plant. Correlation analyses showed that the sulfur content was negatively correlated with soil pH and maize biomass (P < 0.01), and the cadmium content of whole maize was positively correlated with the dry weight of maize (P < 0.05) and the cadmium content in roots and stems (P < 0.01). In summary, to optimize cadmium phytoremediation of the plastic shed soil, an appropriate concentration of sulfur should be selected in practical applications to ensure that the biomass of the maize is maximized, and the cadmium concentration in different parts of the maize is increased or stabilized.


Assuntos
Cádmio/análise , Poluentes do Solo/análise , Biodegradação Ambiental , Biomassa , Monitoramento Ambiental , Humanos , Raízes de Plantas/química , Plásticos , Solo , Enxofre , Zea mays
15.
Ageing Res Rev ; 63: 101155, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32882420

RESUMO

In addition to its role in movement, human skeletal muscle also plays important roles in physiological activities related to metabolism and the endocrine system. Aging and disease onset and progression can induce the reduction of skeletal muscle mass and function, thereby exacerbating skeletal muscle atrophy. Recent studies have confirmed that skeletal muscle atrophy is mainly controlled by the balance between protein synthesis and degradation, the activation of satellite cells, and mitochondrial quality in skeletal muscle. Circadian rhythm is an internal rhythm related to an organism's adaptation to light-dark or day-night cycles of the planet, and consists of a core biological clock and a peripheral biological clock. Skeletal muscle, as the most abundant tissue in the human body, is an essential part of the peripheral biological clock in humans. Increasing evidence has confirmed that maintaining a normal circadian rhythm can be beneficial for increasing protein content, improving mitochondrial quality, and stimulating regeneration and repairing of cells in skeletal muscle to prevent or alleviate skeletal muscle atrophy. In this review, we summarize the roles and underlying mechanisms of circadian rhythm in delaying skeletal muscle atrophy, which will provide a theoretical reference for incorporating aspects of circadian rhythm to the prevention and treatment of skeletal muscle atrophy.

16.
Sleep Med Rev ; 54: 101355, 2020 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-32750654

RESUMO

The aim of this meta-analysis was to compare the pooled sensitivity and specificity of the Pediatric Sleep Questionnaire (PSQ), Obstructive Sleep Apnea Questionnaire (OSA-18), and pulse oximetry (PO) according to OSAS severity. Three electronic databases were searched for studies evaluating sensitivity and specificity of the three tools against the apnea-hypopnea index measured using overnight in-laboratory or in-home polysomnography in children and adolescents from inception until January 11, 2020. A random-effects bivariate model was used to estimate the summary sensitivity and specificity of the tools. We identified 39 studies involving 6131 clinical and community children (aged 2.9-16.7 y). The PSQ exhibited the highest sensitivity (74%) for detecting symptoms of mild pediatric OSAS. The PSQ and PO had comparable sensitivity in screening moderate and severe pediatric OSAS (0.82 and 0.89 vs 0.83 and 0.83, respectively). PO yielded superior specificity in detecting mild, moderate, and severe pediatric OSAS (86%, 75%, and 83%, respectively) than did the PSQ and OSA-18 (all p < 0.05). Age, percentage of girls, index test criteria, methodology quality, and sample size significantly moderated sensitivity and specificity. For early detection of pediatric OSAS, the combined use of PSQ and PO is recommended when polysomnography is not available. PROSPERO REGISTRATION NUMBER: CRD42018090571.

18.
J Headache Pain ; 21(1): 100, 2020 Aug 12.
Artigo em Inglês | MEDLINE | ID: mdl-32787771

RESUMO

BACKGROUND: Psychological distress is highly prevalent among migraineurs during public health emergencies. The coronavirus disease 2019 (nCOV-2019) has created mass panic in China due to its highly contagious by contact and aerosols and lack of effective treatment. However, the emotion status of migraineurs stayed unclear during the nCOV-2019 outbreak. OBJECTIVE: To understand psychological distress of migraineurs by comparing with common population and identify potential high-risk factors of severe psychological distress among migraine patients. METHOD: We enrolled the migraineurs treated at the department of Neurology of West China Hospital and healthy controls with age- and sex-matched to migraineurs. Data on clinicodemographics and psychological distress in the month of February 2020 (during in the nCOV-2019 outbreak in China) were collected. We used the Kessler 6-item (K-6) scale to assess psychological distress. Potential risk factors of severe psychological distress were identified using univariate and multivariate logistic regression. RESULTS: The 144 migraineurs and 150 controls were included in the study. Migraineurs showed significantly higher K-6 scores than controls (P < 0.001). Migraine attack frequency in previous 30 days and time spent paying attention to outbreak showed significant in multivariate logistic regression with respective odds ratios of 2.225 (95%CI 1.361-3.628, P = 0.001) and 1.589 (95% 1.117-2.26, P = 0.01). CONCLUSION: During public health outbreaks, healthcare professionals should focus not only on controlling and reducing migraine attack but also on mental health of migraineurs, especially those with high frequency migraine attack.


Assuntos
Betacoronavirus , Infecções por Coronavirus , Transtornos de Enxaqueca , Pandemias , Pneumonia Viral , Angústia Psicológica , China/epidemiologia , Estudos Transversais , Pessoal de Saúde , Humanos , Prevalência , Fatores de Risco
19.
PLoS Med ; 17(8): e1003188, 2020 08.
Artigo em Inglês | MEDLINE | ID: mdl-32760064

RESUMO

BACKGROUND: The impacts of air pollution on circulatory and respiratory systems have been extensively studied. However, the associations between air pollution exposure and the risk of noncommunicable diseases of other organ systems, including diseases of the digestive, musculoskeletal, and genitourinary systems, remain unclear or inconclusive. We aimed to systematically assess the associations between short-term exposure to main air pollutants (fine particulate matter [PM2.5] and ozone) and cause-specific risk of hospital admission in China over a wide spectrum of human diseases. METHODS AND FINDINGS: Daily data on hospital admissions for primary diagnosis of 14 major and 188 minor disease categories in 252 Chinese cities (107 cities in North China and 145 cities in South China) from January 1, 2013, to December 31, 2017, were obtained from the Hospital Quality Monitoring System of China (covering 387 hospitals in North China and 614 hospitals in South China). We applied a 2-stage analytic approach to assess the associations between air pollution and daily hospital admissions. City-specific associations were estimated with quasi-Poisson regression models and then pooled by random-effects meta-analyses. Each disease category was analyzed separately, and the P values were adjusted for multiple comparisons. A total of 117,338,867 hospital admissions were recorded in the study period. Overall, 51.7% of the hospitalized cases were male, and 71.3% were aged <65 years. Robust positive associations were found between short-term PM2.5 exposure and hospital admissions for 7 major disease categories: (1) endocrine, nutritional, and metabolic diseases; (2) nervous diseases; (3) circulatory diseases; (4) respiratory diseases; (5) digestive diseases; (6) musculoskeletal and connective tissue diseases; and (7) genitourinary diseases. For example, a 10-µg/m3 increase in PM2.5 was associated with a 0.21% (95% CI 0.15% to 0.27%; adjusted P < 0.001) increase in hospital admissions for diseases of the digestive system on the same day in 2-pollutant models (adjusting for ozone). There were 35 minor disease categories significantly positively associated with same-day PM2.5 in both single- and 2-pollutant models, including diabetes mellitus, anemia, intestinal infection, liver diseases, gastrointestinal hemorrhage, renal failure, urinary tract calculus, chronic ulcer of skin, and back problems. The association between short-term ozone exposure and respiratory diseases was robust. No safety threshold in the exposure-response relationships between PM2.5 and hospital admissions was observed. The main limitations of the present study included the unavailability of data on personal air pollution exposures. CONCLUSIONS: In the Chinese population during 2013-2017, short-term exposure to air pollution, especially PM2.5, was associated with increased risk of hospitalization for diseases of multiple organ systems, including certain diseases of the digestive, musculoskeletal, and genitourinary systems; many of these associations are important but still not fully recognized. The effect estimates and exposure-response relationships can inform policy making aimed at protecting public health from air pollution in China.


Assuntos
Poluição do Ar/efeitos adversos , Poluição do Ar/análise , Material Particulado/efeitos adversos , Material Particulado/análise , Admissão do Paciente/tendências , Idoso , Idoso de 80 Anos ou mais , China/epidemiologia , Bases de Dados Factuais/tendências , Feminino , Hospitalização/tendências , Humanos , Masculino , Pessoa de Meia-Idade , Fatores de Risco , Fatores de Tempo
20.
Hu Li Za Zhi ; 67(4): 50-60, 2020 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-32748379

RESUMO

BACKGROUND: Truth-telling is an important step toward reducing the cognitive gap between physicians and patients as well as reducing the psychological pressures applied to physicians by family members. There is a lack of research on the truth-telling experience and needs in the intensive care unit from the perspective of patient family members. PURPOSE: This study is designed to explore the experiences and needs of families in the intensive care unit. METHODS: A descriptive phenomenology method was used in this study. In-depth interviews were conducted with five participants who had family members assessed with acute physiology and chronic health evaluation II scores ≥ 20. Data were analyzed using Giorgi's phenomenological methods and Nvivo 11. RESULTS: Four experience themes were examined, including (1) nothing is clear, requires explanation; (2) helpless to find answers, need a nurse to resolve this issue; (3) professional conduct makes us feel helpless, longing for love from the medical team; (4) decisions are very difficult, hoping to get more help. CONCLUSIONS / IMPLICATIONS FOR PRACTICE: The family members expressed that they were unable to understand the underlying causes of the progression in patient condition because the medical team only presented outcomes to the family and did not discuss related causes. Thus, it is recommended that medical teams learn to recognize the cognitive processes of patient family members and consider their emotions, including their needs and expectations, in order to provide individualized explanations based on a patient's status and progress.


Assuntos
Família/psicologia , Unidades de Terapia Intensiva , Médicos/psicologia , Relações Profissional-Família , Revelação da Verdade , Humanos , Determinação de Necessidades de Cuidados de Saúde , Pesquisa Qualitativa
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