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1.
Chin Med J (Engl) ; 134(23): 2799-2809, 2021 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-34759226

RESUMO

ABSTRACT: In the past 37 years, human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS) has undergone various major transmission routes in China, with the world most complex co-circulating HIV-1 subtypes, even the prevalence is still low. In response to the first epidemic outbreak of HIV in injecting drug users and the second one by illegal commercial blood collection, China issued the Anti-Drug Law and launched the Blood Donation Act and nationwide nucleic acid testing, which has avoided 98,232 to 211,200 estimated infections and almost ended the blood product-related infection. China has been providing free antiretroviral therapy (ART) since 2003, which covered >80% of the identified patients and achieved a viral suppression rate of 91%. To bend the curve of increasing the disease burden of HIV and finally end the epidemic, China should consider constraining HIV spread through sexual transmission, narrowing the gaps in identifying HIV cases, and the long-term effectiveness and safety of ART in the future.

2.
Sci Total Environ ; 767: 144340, 2021 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-33429273

RESUMO

Phragmites australis and Chloris virgata are native, dominant, salt-tolerant grass species that grow in the Yellow River Delta, China, and have potential applications in the phytoremediation of petroleum-polluted saline soil. The characteristics of endophytic bacterial communities of Phragmites australis and Chloris virgata and their functions in hydrocarbon degradation and plant growth promotion have been studied using both high-throughput sequencing and conventional microbial techniques. Through 16S rRNA gene amplicon sequencing, we found five bacterial phyla that were dominant among the endophytic bacterial communities of the two grass species, including Proteobacteria, Actinobacteria, Firmicutes, Bacteroidetes, and Tenericutes. The phylum Proteobacteria was common among the endophytic bacterial communities of the two grass species. The diversity in the endophytic bacterial community of Chloris virgata was generally higher than that in the community of Phragmites australis. Thirty-eight hydrocarbon-degrading endophytic bacteria were isolated from the two grasses via culturing techniques. Based on phylogenetic analyses, the bacterial isolates were classified into the phyla Proteobacteria, Firmicutes, and Actinobacteria. The majority of strains belonged to the genera Bacillus and Pseudomonas. More than 70% of the isolates of hydrocarbon-degrading endophytes exhibited the ability to stimulate plant growth. These isolates mainly belonged to Bacillus sp., Pseudomonas sp., Beijerinckia sp., Serratia sp., Acinetobacter sp., Microbacterium sp., and Rhizobium sp. Altogether, the present study revealed that Phragmites australis and Chloris virgata growing on petroleum-polluted saline soil in the Yellow River Delta harbor several diverse species of endophytic bacteria and serve as novel sources of beneficial bacteria and hydrocarbon degradation.


Assuntos
Poaceae , Rios , Bactérias/genética , China , Filogenia , RNA Ribossômico 16S/genética
3.
Forensic Sci Int Genet ; 51: 102424, 2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-33248347

RESUMO

Short tandem repeat (STR) profiling is routinely used in forensic genetics. At present, STR analysis is mainly performed by capillary electrophoresis (CE). However, due to limitations associated with the CE method, STR genotyping has been limited to length polymorphisms only. Because next generation sequencing (NGS) is capable of providing full resolution STR data at the sequence variation level, the individual identification capability of forensic STR loci could be significantly improved. Here we present sequence-based STR data for the Beijing Han population in which 291 individuals were screened for 23 commonly used forensic STRs using the SeqTypeR24 CASE kit on an Ion PGM platform. In total, 234 length-based alleles and 356 sequence-based alleles, which included 22 novel core repeat sequences, were observed. The sequence-based matching probability and power of discrimination were superior to the length-based numbers for 16 loci bearing micro-variant alleles. Combined matching probability reached 8.2 × 10-29 for 23 STR loci at the sequence level. This was two orders of magnitude higher than the parameters at length level and provides a data base for sequence-based STR casework applications.


Assuntos
Alelos , Variação Genética , Repetições de Microssatélites , China , Impressões Digitais de DNA , Genética Populacional , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Reação em Cadeia da Polimerase , Análise de Sequência de DNA
4.
Bioengineered ; 12(1): 278-285, 2021 12.
Artigo em Inglês | MEDLINE | ID: mdl-33356806

RESUMO

Enhanced serum secreted clusterin (sCLU) protein was associated with progression, poor prognosis and chemotherapy sensitivity evaluation in malignant patients. However, the clinical significance of serum sCLU protein levels in patients with invasive breast cancer (IBC) is unknown. In this study, the serum sCLU protein in 2648 patients with IBC was detected. The diagnostic value and treatment responses of serum sCLU protein in patients with IBC were also performed. The results showed that the serum sCLU protein level was significantly higher in IBC patients compared to the healthy controls (P < 0.0001), and strongly correlated with higher clinical tumor stage (P < 0.001), lymph node metastasis (P < 0.001), shorter overall survival (OS) (P = 0.032) and disease-free survival (DFS) (P = 0.029), respectively. Using the cutoff value of 18.46 µg/mL, the sensitivity and specificity were 86.26% and 73.46% to separate IBC patients from noncancerous and healthy controls. The postoperative patients showed lower serum sCLU levels compared to the preoperative patients (P = 0.003). The chemoresistant patients showed higher serum sCLU levels compared to the chemosensitive patients (P < 0.001). These data indicated that serum sCLU levels are effective indicators for diagnosis and chemotherapy sensitivity evaluation in patients with IBC.


Assuntos
Neoplasias da Mama , Clusterina/sangue , Neoplasias da Mama/sangue , Neoplasias da Mama/diagnóstico , Neoplasias da Mama/tratamento farmacológico , Neoplasias da Mama/mortalidade , Feminino , Humanos , Pessoa de Meia-Idade , Prognóstico , Resultado do Tratamento
5.
Clin Lab ; 66(8)2020 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-32776747

RESUMO

BACKGROUND: The relationship between non-cholestatic liver disease and total bile acid (TBA) remains obscure. The present study aimed to verify this relationship in patients with non-cholestatic chronic hepatitis B virus (HBV) infection. METHODS: A total of 922 consecutive chronic HBV infected patients with alkaline phosphatase (ALP) ≤ 1.5 upper limit of normal (ULN) and gamma-glutamyl transferase (GGT) ≤ 3 ULN were rigorously included in this cross-sectional study. Liver biopsy was performed in 53 patients and Scheuer scoring system was used to evaluate inflammation grade. G3/G4 or Child-Pugh B/C were considered to be significant liver injury. RESULTS: Compared to Child-Pugh A, TBA, total bilirubin (TBIL), ALP, aspartate aminotransferase (AST), alanine aminotransferase (ALT), and AST to ALT ratio (AST/ALT) were significantly higher in Child-Pugh B/C, while TBIL to TBA ratio (TBIL/TBA) was significantly lower (all p < 0.001). In multivariate analysis, TBA and AST/ALT were independently correlated with Child-Pugh B/C [odds ratio (OR) = 1.04, p < 0.001; OR = 1.79, p < 0.001, respectively]. The area under the curve (AUC) of TBA (0.82) was significantly higher than that of AST (0.73, p < 0.001) and ALT (0.63, p < 0.001). Furthermore, in patients with liver biopsy, TBA was also significantly higher in G3/G4 while TBIL/TBA was significantly lower (p < 0.05). After adjusting the factors related to bile excretion, TBIL/TBA was independently associated with G3/G4 (OR = 0.89, p = 0.037). CONCLUSIONS: Serum TBA shows a close relationship with significant liver injury in chronic HBV infected patients without cholestasis. Assessment of TBA, especially in combination with TBIL/TBA, may serve as a non-invasive marker for the diagnosis of non-cholestatic hepatic damage.


Assuntos
Colestase , Hepatite B Crônica , Alanina Transaminase , Ácidos e Sais Biliares , Estudos Transversais , Hepatite B Crônica/diagnóstico , Humanos , Fígado
6.
Exp Ther Med ; 20(1): 243-250, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32550883

RESUMO

Although the platelet count may provide clues regarding the severity of liver disease, there are currently no available data supporting the utility of the platelet count to evaluate the degree of liver injury in patients with chronic hepatitis B virus (HBV) infection. The present study aimed to determine the association between the platelet count and the severity of liver injury in patients with chronic HBV infection. A total of 941 patients were included and were stratified into a Child-Turcotte-Pugh (CTP) class A group and a CTP class B/C group using the CTP scoring system. A total of 53 patients underwent liver biopsy. The pathological stage F4 was defined as cirrhosis based on the METAVIR scoring system. Compared with that in patients with CTP class A, the platelet count in patients with CTP class B/C was lower (P<0.001). Similarly, for patients with normal alanine aminotransferase (ALT) levels, the platelet count was significantly different between the CTP class B/C and A groups (P<0.001). The platelet count was inversely correlated with the CTP score (r=-0.420, P<0.001) and independently associated with CTP grade B/C [odds ratio (OR), 0.994; 95% CI, 0.990-0.999; P=0.009]. The area under the receiver operating characteristic curve (AUC) of the platelet count to distinguish CTP grade B/C from A was 0.712 and 0.791, respectively, in all patients with HBV infection and the subset with normal ALT levels. In addition, compared to patients with chronic hepatitis B, patients with cirrhosis had a lower platelet count and higher aspartate transaminase-to-platelet ratio index (APRI) and fibrosis index based on four factors (FIB-4) (P<0.001). The platelet count was inversely correlated with FIB-4 (r=-0.855, P<0.001) and APRI (r=-0.741, P<0.001). The AUC for the platelet count to distinguish cirrhosis from chronic hepatitis B was 0.927 (sensitivity, 78.76%; specificity, 92.22%). Among patients who underwent liver biopsy, the platelet count in those with F4 was lower compared with that in patients with ≤F3 (P=0.013). The platelet count was inversely correlated with the pathological stage (r=-0.295, P=0.032) and was independently associated with F4 (OR, 0.978; 95% CI, 0.960-0.997; P=0.026). The AUC of the platelet count to distinguish F4 from patients with ≤F3 was 0.761. In conclusion, the platelet count may be used as a non-invasive marker to assess the severity of liver injury and of liver fibrosis in patients with chronic HBV infection.

7.
Medicine (Baltimore) ; 99(8): e19248, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32080129

RESUMO

Although serum bile acids and total cholesterol (TC) are closely related to liver cirrhosis, the potential diagnostic value of total bile acid-to-cholesterol ratio (TBA/TC) for liver fibrosis is unclear. The present study aimed to evaluate the value of TBA/TC in the diagnosis of cirrhosis and the relationship between TBA/TC and significant liver fibrosis in chronic hepatitis B virus (HBV) infected patients without cholestasis.667 patients with alkaline phosphatase (ALP) ≤ 1.5 upper limit of normal (ULN) and gamma-glutamyl transferase (GGT) ≤ 3 ULN were rigorously included in this cross-sectional study. Liver biopsy was performed in 32 patients and METAVIR scoring system was used to evaluate liver fibrosis stage. Liver ultrasound elastography was performed in 138 patients, significant fibrosis was defined as fibrosis ≥ F2. Multiple logistic regression as well as receiver operating characteristic (ROC) curves analyses were performed.Compared to patients with non-cirrhosis, TBA and TBA/TC were significantly higher in cirrhosis while TC was significantly lower (all P < .001). In multivariate analysis, TBA/TC was also independently associated with cirrhosis [odds ratio (OR) = 1.102, 95% confidence interval (CI): 1.085-1.166]. The area under the curve (AUC) of TBA/TC (0.87) was almost equivalent to the aspartate aminotransferase to platelet ratio index (APRI, AUC = 0.84) and fibrosis 4 score (FIB-4, AUC = 0.80), and the optimal cut-off value for TBA/TC to diagnose cirrhosis was 2.70. Among the patients performed liver biopsy, TBA/TC were significantly higher both in significant fibrosis and cirrhosis as well as significantly correlated with fibrosis stage (all P < .001). Furthermore, In patients performed liver ultrasound elastography, TBA/TC was also independently associated with significant fibrosis (OR = 1.040, 95% CI: 1.001-1.078).Assessment of TBA/TC could serve as an additional marker of significant liver fibrosis and cirrhosis in non-cholestatic chronic HBV infection.


Assuntos
Ácidos e Sais Biliares/sangue , Colesterol/sangue , Hepatite B Crônica/complicações , Cirrose Hepática/etiologia , Adulto , Biomarcadores , Biópsia , Estudos Transversais , Técnicas de Imagem por Elasticidade , Feminino , Hepatite B Crônica/sangue , Humanos , Cirrose Hepática/sangue , Testes de Função Hepática , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Curva ROC , Índice de Gravidade de Doença
8.
Sci Rep ; 10(1): 1945, 2020 02 06.
Artigo em Inglês | MEDLINE | ID: mdl-32029845

RESUMO

Microhaplotypes are an emerging type of forensic genetic marker that are expected to support multiple forensic applications. Here, we developed a 124-plex panel for microhaplotype genotyping based on next-generation sequencing (NGS). The panel yielded intralocus and interlocus balanced sequencing data with a high percentage of effective reads. A full genotype was determined with as little as 0.1 ng of input DNA. Parallel mixture experiments and in-depth comparative analyses were performed with capillary-electrophoresis-based short tandem repeat (STR) and NGS-based microhaplotype genotyping, and demonstrated that microhaplotypes are far superior to STRs for mixture deconvolution. DNA from Han Chinese individuals (n = 256) was sequenced with the 124-plex panel. In total, 514 alleles were observed, and the forensic genetic parameters were calculated. A comparison of the forensic parameters for the 20 microhaplotypes with the top Ae values in the 124-plex panel and 20 commonly used forensic STRs showed that these microhaplotypes were as effective as STRs in identifying individuals. A linkage disequilibrium analysis showed that 106 of the 124 microhaplotypes were independently hereditary, and the combined match probability for these 106 microhaplotypes was 5.23 × 10-66. We conclude that this 124-plex microhaplotype panel is a powerful tool for forensic applications.


Assuntos
Genética Forense , Haplótipos/genética , Polimorfismo de Nucleotídeo Único/genética , Alelos , DNA/genética , Impressões Digitais de DNA/métodos , Genótipo , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Humanos , Desequilíbrio de Ligação/genética , Repetições de Microssatélites/genética , Probabilidade , Análise de Sequência de DNA/métodos
9.
Medicine (Baltimore) ; 96(14): e6535, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28383420

RESUMO

This article investigated the feasibility of laparoscopic surgery in unfavorable site gastric gastrointestinal stromal tumors (GISTs).We identified 214 patients who underwent primary gastric GIST resection at our institution (January 2006-December 2014) from a prospectively collected database. These patients were divided into a Favorable group (140 cases) and an Unfavorable group (74 cases) according to the 2014 version of the National Comprehensive Cancer Network Clinical Guidelines (NCCN guidelines).The wedge resection rate of the Favorable group was higher than that of the Unfavorable group, and most procedures were performed laparoscopically (P < 0.05). In addition, there were no differences in the other clinicopathological features between these groups (P > 0.05). The postoperative stay of the Unfavorable group was longer than that of the Favorable group (P = 0.02). Laparoscopic surgery in both groups resulted in a shorter operative time, lower blood loss, faster time to first flatus, faster time to first fluid diet, and shorter postoperative stay than open surgery (P < 0.05). Although the difference was not significant (P = 0.09), the postoperative complication incidence of the Favorable group was less than that of the Unfavorable group (10% vs 17.6%). Furthermore, in the Unfavorable group, the incidence of postoperative complications from laparoscopic surgery was significantly lower than that of open surgery (P = 0.001). There were no differences in the 5-year overall survival (OS) and recurrence-free survival (RFS) of these groups (P > 0.05). Furthermore, in the Unfavorable group, the 5-year OS and RFS were similar for both laparoscopic and open procedures. Multivariate Cox regression analysis showed that imatinib (IM) treatment was an independent risk factor for poor prognosis.Laparoscopic operation for gastric GISTs located in unfavorable sites can yield similar long-term outcomes compared with an open operation. However, laparoscopic surgery has the obvious advantage of being minimally invasive, and the incidence of postoperative complications was low. Laparoscopic surgery is thus an option for the treatment of localized gastric GISTs.


Assuntos
Neoplasias Gastrointestinais/cirurgia , Tumores do Estroma Gastrointestinal/cirurgia , Laparoscopia/estatística & dados numéricos , Estômago/cirurgia , Adulto , Idoso , China/epidemiologia , Estudos de Coortes , Feminino , Neoplasias Gastrointestinais/patologia , Tumores do Estroma Gastrointestinal/patologia , Humanos , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Complicações Pós-Operatórias/epidemiologia , Melhoria de Qualidade , Estômago/patologia , Resultado do Tratamento
10.
J Cancer Res Clin Oncol ; 143(4): 727-734, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28083710

RESUMO

BACKGROUND: The therapy for gastrointestinal stromal tumors (GIST) has changed significantly since the use of imatinib mesylate (IM). However, the appropriate duration of receiving adjuvant IM for patients with high-risk GIST who underwent R0 resection is still controversial. METHODS: From January 2005 to December 2014, 234 patients who underwent R0 resection and were treated with adjuvant imatinib at our institution were identified from a prospectively collected database. The effect of the medication duration on the long-term outcomes was analyzed. RESULTS: In this study, 140 cases were male and 94 cases were female, and the mean age was 57.5 ± 11.4 years. The most common site was the stomach (103 cases, 44%), followed by the small intestine (81 cases, 34.6%). The 5 year recurrence-free survival (RFS) rate and overall survival (OS) rate in the whole groups were 76.2 and 83.4%, respectively. The patient's prognosis was improved due to the prolongation of the time of receiving the imatinib treatment (P < 0.05). According to the results of the risk stratification analysis, the outcomes of the moderate-risk patients who received IM adjuvant therapy for 1-year group, 1-3 years group and more than 3 years group showed improvement, but the difference was not statistically significant (P > 0.05). However, in the high-risk patients, the RFS rates of the 1-year group, 1-3-years group, 3-5-years group and more than 5 years group were 36.5, 68.7, 71.2 and 90.8%, respectively, and the OS rates were 36.7, 76.6, 84.0 and 97.4%, respectively (P < 0.001). In addition, linear regression analysis showed that the long-term outcomes of patients with high-risk GIST significantly improved due to prolonged adjuvant IM treatment durations (P < 0.05). The RFS rate of patients receiving IM for more than 5 years was significantly better than those receiving it for less than 5 years. Multivariate COX regression analysis in the patients with high-risk GIST showed that tumor located in small intestine was an independent risk factor, while receiving IM treatment was an independent protective factor for prognosis. CONCLUSIONS: The long-term outcomes of patients with high risk GIST improved due to the prolongation of the IM treatment. To reduce the recurrence and improve the long-term survival, we suggest that patients with high-risk GIST receive imatinib treatment for at least 5 years.


Assuntos
Tumores do Estroma Gastrointestinal/tratamento farmacológico , Mesilato de Imatinib/uso terapêutico , Antineoplásicos , Quimioterapia Adjuvante , Seguimentos , Humanos
11.
Medicine (Baltimore) ; 95(15): e3135, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-27082552

RESUMO

Published reports on laparoscopic resection of gastric gastrointestinal stromal tumor (GIST) were limited to small experiences and selection bias. Two hundred fourteen patients who underwent primary gastric GIST resection at our institution (January 2006-December 2012) were identified from a prospectively collected database. Laparoscopic resections (LAP) were performed in 133 patients, and open resections (OPEN) were performed in 81 patients. The short- and long-term outcomes were analyzed using propensity-score matching (PSM) by comparing the clinicopathological factors between these groups. The tumor resection method and tumor size were significantly different between the LAP and OPEN groups. After PSM, there were no differences (P > 0.05) in these clinicopathological factors. The LAP group had less blood loss and shorter operation time, time to first flatus, time to first fluid diet, time to gastric tube removal, and postoperative stay before PSM. In addition, there were no differences regarding the time of drainage tube removal or hospitalization expense. Other than the time of gastric tube removal, which was similar in these 2 groups, the short-term outcomes were similar before and after PSM. The rates of postoperative complications in the LAP and OPEN groups were 6.8% and 22.8%, respectively, before PSM (P = 0.001) and 5.6% and 22.5%, respectively, after PSM (P = 0.004). The multivariate analyses for complications showed that tumors were located in the middle of the stomach, and the operation method and proximal gastrectomy were independent risk factors before and after PSM. The 5-year cumulative survival rates in the LAP and OPEN groups were 95.4% and 85.9%, respectively, (P = 0.07) before PSM and 93.1% and 91.9%, respectively, (P = 0.69) after PSM (not significantly different). Laparoscopic resection for gastric GISTs had better short-term outcomes and similar long-term outcomes compared with open surgery. Localized gastric GISTs can be treated with laparoscopic surgery.


Assuntos
Gastrectomia/métodos , Gastrectomia/estatística & dados numéricos , Tumores do Estroma Gastrointestinal/cirurgia , Laparoscopia/métodos , Laparoscopia/estatística & dados numéricos , Neoplasias Gástricas/cirurgia , Adulto , Idoso , Feminino , Humanos , Tempo de Internação , Masculino , Pessoa de Meia-Idade , Duração da Cirurgia , Complicações Pós-Operatórias/epidemiologia , Pontuação de Propensão , Fatores de Tempo
12.
Mol Med Rep ; 12(5): 7227-32, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26458849

RESUMO

Bone marrow-derived mesenchymal stem cells (BM-MSCs) are considered to be a potential therapy for end-stage liver disease. However, the therapeutic mechanism of BM-MSCs remains unclear. The aim of the current study was to investigate the role of paracrine signaling in BM­MSCs in liver cirrhosis in vitro. Human BM­MSCs and hepatic stellate cells (HSCs) were cultured using a vertical double cell co­culture system. Groups were divided into HSCs alone (control group) and the co­culture system of BM­MSCs with HSCs (experimental group). HSC morphology was observed by inverted phase contrast microscopy. The proliferative capacity of HSCs was measured with the MTT assay and flow cytometry. Hoechst staining was performed to examine the apoptosis of HSCs. Transforming growth factor (TGF)­ß1 and Smad7 mRNA expression were detected by reverse transcription­quantitative polymerase chain reaction and western blotting. BM­MSCs did not inhibit the proliferation of HSCs at 24 h, however significantly inhibited the proliferation of HSCs at 48 and 72 h. BM­MSCs additionally induced the apoptosis of HSCs at 48 h. The concentration of TGF­ß1 in the supernatant at 24 h and 48 h in the co­cultured system was observed to be significantly lower than in the control group (P<0.05). The level of TGF­ß1 mRNA in the experimental group at 48 h was significantly lower than the control group, however Smad7 mRNA levels were significantly greater than in the control group. Additionally, TGF­ß1 protein levels were significantly lower than in the control group, however levels of Smad7 were greater than the control group. It was concluded that BM­MSCs are able to inhibit the proliferation and promote the apoptosis of HSCs. In addition, the mechanism may be associated with inhibition of the TGF-ß1/Smad pathway in HSCs.


Assuntos
Células Estreladas do Fígado/fisiologia , Células-Tronco Mesenquimais/fisiologia , Fator de Crescimento Transformador beta1/fisiologia , Apoptose , Comunicação Celular , Linhagem Celular , Proliferação de Células , Técnicas de Cocultura , Expressão Gênica , Humanos , Transdução de Sinais , Proteína Smad7/genética , Proteína Smad7/metabolismo
13.
Huan Jing Ke Xue ; 32(5): 1340-5, 2011 May.
Artigo em Chinês | MEDLINE | ID: mdl-21780588

RESUMO

To study the purification mechanism and biogeochemical cycle of wetlands, the concentration of Fe/Mn and oxidation reduction potential (ORP) in the pore water were monitored in situ on the Wuhan Zoo stormwater wetland from 2004 to 2006. The results showed that the water level of the wetland was changed from -10 cm to 30 cm, and the range of ORP was-120-220 mV. The elements of sulfur, iron and manganese were the main oxidation and reduction systems in the wetland. The ranges of total Fe, Mn and Fe2+ concentration were 0.60-2.35, 0.75-1.89 and 0.20-1.25 mg x L(-1), respectively. The concentrations of total Fe, Mn and Fe2+ were higher in spring and summer than that in autumn and winter, while the sulfate concentration was on the contrary and the ratio of Fe2+/Fe was slightly various in different seasons. From the surface of 10 cm to 50 cm underground, ORP and pH was decreased, whereas the concentrations of total Fe, Mn and Fe2+ were increased (except for the surface water). Through the correlation analysis, it was showed that the correlation of ORP and total Mn and Fe2+ was negative and significant, while there was no significant correlation between ORP and total Fe (R = -0.169, p < 0.0001). The order of the correlation coefficient was total Mn, Fe and Fe2+ in turn. Therefore, the systems of sulfur, iron and manganese were the important component of the oxidation and reduction system for stormwater wetland.


Assuntos
Ferro/análise , Manganês/análise , Chuva , Poluentes Químicos da Água/análise , Áreas Alagadas , Biodegradação Ambiental , China , Monitoramento Ambiental , Movimentos da Água , Purificação da Água/métodos
14.
Biomed Environ Sci ; 23(5): 357-62, 2010 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-21112483

RESUMO

OBJECTIVES: This paper aims to investigate the uterotrophic activities of lactational exposure to combination of soy isoflavones (SIF) and bisphenol A (BPA) and to examine estrogen receptor α (ERα) and estrogen receptor ß (ERß) expressions in hypothalamus-pituitary-ovary axis and uterus. METHODS: Maternal rats that were breeding about 8 litters were randomly divided into four groups with seven dams in each group. Dams in different treatment groups received corn oil (control), 150 mg/kg BW of SIF, 150 mg/kg BW of BPA or combination of 150 mg/kg BW of SIF and 150 mg/kg BW of BPA, respectively, from postnatal day 5 to 11 (PND5-11) by gavage. On PND12 and PND70, 10 female litters were killed and hypothalamus, pituitary, ovary and uterus were collected. ERα and ERß expressions in these organs were detected with Western blotting assay. And vaginal opening time and estrus cycle were examined in animals fed for PND70. RESULTS: On PND12, the relative uterine weight of rats treated with ISF or BPA or their combination was significantly higher than that of untreated rats (P<0.05). But the relative uterine weight of rats in the co-exposure group was slightly lower than that in the group only exposed to SIF or BPA. On PND 70, however, the relative uterine weight in each treatment group was not statistically different from that in the control group (P>0.05). Vaginal opening time and estrus cycle in groups treated with SIF or BPA or their combination were similar to those in the control group (P>0.05). Exposure to SIF or BPA or their combination could up-regulate or down-regulate ERα and ERß expressions in hypothalamus, pituitary, ovary and uterus on PND12 and PND70. These regulation patterns for ERα and ERß were different in different organs at different time points. CONCLUSION: Lactational exposure to ISF or BPA or their combination could induce uterotrophic responses in neonate rats, which disappeared in later life. But these data fail to suggest a possibility for synergic actions between SIF and BPA. It was also demonstrated that the uterotrophic effects of SIF and BPA exposure might, at least, involve modification of ERα or ERß expressions in the hypothalamus-pituitary-ovary axis.


Assuntos
Receptor alfa de Estrogênio/biossíntese , Receptor beta de Estrogênio/biossíntese , Estrogênios não Esteroides , Sistema Hipotálamo-Hipofisário/efeitos dos fármacos , Lactação , Ovário/efeitos dos fármacos , Soja/química , Animais , Animais Recém-Nascidos , Compostos Benzidrílicos , Western Blotting , Regulação para Baixo , Sinergismo Farmacológico , Estrogênios não Esteroides/farmacocinética , Estrogênios não Esteroides/toxicidade , Feminino , Sistema Hipotálamo-Hipofisário/metabolismo , Isoflavonas/isolamento & purificação , Isoflavonas/farmacocinética , Isoflavonas/toxicidade , Lactação/metabolismo , Exposição Materna , Tamanho do Órgão/efeitos dos fármacos , Ovário/metabolismo , Fenóis/farmacocinética , Fenóis/toxicidade , Fitoestrógenos/isolamento & purificação , Fitoestrógenos/farmacocinética , Fitoestrógenos/toxicidade , Ratos , Ratos Sprague-Dawley , Maturidade Sexual/efeitos dos fármacos , Regulação para Cima , Útero/efeitos dos fármacos , Útero/metabolismo
15.
J Sep Sci ; 32(7): 1069-74, 2009 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-19266545

RESUMO

In this paper, solid-phase extraction (SPE) in combination with dispersive liquid-liquid microextraction (DLLME) has been developed as a sample pretreatment method with high enrichment factors for the sensitive determination of amide herbicides in water samples. In SPE-DLLME, amide herbicides were adsorbed quantitatively from a large volume of aqueous samples (100 mL) onto a multiwalled carbon nanotube adsorbent (100 mg). After elution of the target compounds from the adsorbent with acetone, the DLLME technique was performed on the resulting solution. Finally, the analytes in the extraction solvent were determined by gas chromatography-mass spectrometry. Some important extraction parameters, such as flow rate of sample, breakthrough volume, sample pH, type and volume of the elution solvent, as well as salt addition, were studied and optimized in detail. Under optimum conditions, high enrichment factors ranging from 6593 to 7873 were achieved in less than 10 min. There was linearity over the range of 0.01-10 microg/L with relative standard deviations of 2.6-8.7%. The limits of detection ranged from 0.002 to 0.006 microg/L. The proposed method was used for the analysis of water samples, and satisfactory results were achieved.

16.
J Environ Sci (China) ; 19(9): 1067-73, 2007.
Artigo em Inglês | MEDLINE | ID: mdl-17966511

RESUMO

Pollution in urban zoos arises from diffuse and small point sources. However, its control has received little attention in past decades. Online and offline modes of ecological engineering technology were designed to control pollution from small point and diffuse sources in Wuhan City Zoo, China. Their characteristics and performances were investigated in sixteen runoff events from 2003 to 2005. The results showed that the two modes both improved runoff water quality and had high retention rates for water and pollutants. In the outflows, the event mean concentrations (EMCs) of total suspended solids (TSS), chemical oxygen demand (COD), total nitrogen (TN) and total phosphorus (TP) were reduced by 88%, 59%, 46% and 71% for the online mode, and those were 77%, 42%, 50% and 66% for the offline mode. The annual retention rates of pollutant loads for the online mode were 94.9%-98.5% in the three study years; those for the offline mode were 70.5%-86.4%. Based on calculation, the online mode was able to store the runoff of 66.7 mm rainfall completely, and the offline mode could store that of 31.3 mm rainfall. In addition, the online mode can provide an effective way for rainwater utilization and good habitats for aquatic wildlives, and has an excellent aesthetics value for recreationsal pastimes. The offline mode can save land resources and may be an effective and economical measure for diffuse pollution control in urban areas.


Assuntos
Recuperação e Remediação Ambiental/métodos , Recreação , Poluentes da Água , China , Áreas Alagadas
17.
Huan Jing Ke Xue ; 28(10): 2280-6, 2007 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-18268993

RESUMO

9 kinds of substrate materials (gravel, aluminite stone, sands, soil, zeolite, ceramic granule, limestone, steel slag and vermiculite) were selected to examine the phosphorus adsorption and removal capacities from runoff by adsorption, captive test and dynamic adsorption experiments. The results showed that these substrate materials had higher removal efficiency for PO4(3-) than those of other phosphorus forms. The adsorption characteristics of the substrates could be described by both Freundlich and Langmuir adsorption isotherms. The equilibrium adsorption capacity of these substrates was in the following order: zeolite, soil and vermiculite > ceramic granule, steel slag and limestone > gravel, sands and aluminite stone. The dynamic adsorption capacity was zeolite, soil, limeramic granule, limestone > steel slag, gravel and sands > ceramic granule and vermiculite. In the meanwhile, the releasing phenomena of phosphorus also could be observed in the captive and dynamic test, particularly for vermiculite, ceramic granule and steel slag. In the dynamic adsorption experiment, the removal efficiency of P-pollutants was ranged from 30% to 87% for the substrate materials except vermiculite, ceramic granule and steel slag. The results suggest that zeolite, limestone and soil are appropriate substrates for removing P-pollutants from stormwater.


Assuntos
Fósforo/química , Eliminação de Resíduos Líquidos/métodos , Poluentes Químicos da Água/química , Zeolitas/química , Adsorção , Carbonato de Cálcio/química , Fosfatos/química , Fosfatos/isolamento & purificação , Fósforo/isolamento & purificação , Solo , Poluentes Químicos da Água/isolamento & purificação , Purificação da Água/métodos
18.
Ying Yong Sheng Tai Xue Bao ; 18(10): 2319-25, 2007 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-18163317

RESUMO

An off-line filtering ditch-pond system for controlling storm runoff pollution in urban tourist areas was developed, which could retain the first flush effectively, resulting in the decrease of pollutant concentration and suspended solid average grain size, and the improvement of pollutant retention in runoff. This system could be an effective treatment system for storm runoff pollution, particularly for the scarcity of available land use in urban areas. In 2005, the yearly retention rates of TSS, COD, TN and TP were 86.4%, 85.5%, 83.9% and 82.9%, and during a storm event on June 26, the retention rates of runoff volume, TSS, COD, TN and TP were 67.9%, 97.0%, 89.2%, 94.9% and 96.2%, respectively. This system could also retain most of the suspended solids in runoff.


Assuntos
Chuva , Movimentos da Água , Poluentes Químicos da Água/análise , Poluição da Água/prevenção & controle , China , Cidades , Filtração/instrumentação , Humanos , Viagem , Poluição da Água/análise
19.
World J Gastroenterol ; 12(3): 482-4, 2006 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-16489655

RESUMO

AIM: To investigate the relationship between serum soluble interleukin-2 receptor (sIL-2R) level and anti-HBc in patients with chronic hepatitis B virus (HBV) infection. METHODS: Sera from 100 patients with chronic HBV infection and 30 healthy controls were included in this study. The patients were divided into group A [HBsAg (+), HBeAg (+) and anti-HBc (+), n=50] and group B [HBsAg (+), HBeAg (+) and anti-HBc (-), n=50]. sIL-2R levels were determined using ELISA. HBV DNA and alanine aminotransferase (ALT) were also detected. RESULTS: Serum sIL-2R levels were significantly higher in patients with chronic HBV infection than in healthy controls. Moreover, serum sIL-2R levels were significantly higher in patients with HBsAg (+), HBeAg (+) and anti-HBc (+) (976.56+/-213.51x10(3) U/L) than in patients with HBsAg (+), HBeAg (+) and anti-HBc (-) (393.41+/-189.54x10(3) U/L, P<0.01). A significant relationship was found between serum sIL-2R and ALT levels (P<0.01) in patients with chronic HBV infection, but there was no correlation between sIL-2R and HBV DNA levels. The anti-HBc status was significantly related to the age of patients (P<0.01). CONCLUSION: The high sIL-2R level is related to positive anti-HBc in chronic hepatitis B patients. Positive anti-HBc may be related to T-lymphocyte activation and negative anti-HBc may imply immune tolerance in these patients.


Assuntos
Anticorpos Anti-Hepatite B/imunologia , Hepatite B Crônica/sangue , Fatores Imunológicos/imunologia , Receptores de Interleucina-2/sangue , Adolescente , Adulto , Idoso , Alanina Transaminase/metabolismo , Criança , Pré-Escolar , Vírus da Hepatite B/imunologia , Hepatite B Crônica/imunologia , Humanos , Pessoa de Meia-Idade , Receptores de Interleucina-2/imunologia
20.
J Hazard Mater ; 132(2-3): 261-8, 2006 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-16297544

RESUMO

The low level detection of air pollutants requires reliable sampling and calibration techniques, as well as using sophisticated analytical instrumentation. Incorporating these requirements into a mobile platform allows for accurate, curb side measurement of airborne ambient chemicals in real time. In this case study, air emissions from two manufacturing facilities in close proximity to each other were monitored with a mobile unit to chemically characterize and differentiate the emissions from each facility. Based upon the downwind fingerprinting and concentration levels, the mobile unit was able to isolate the source of the emissions to the responsible facility. A few chemicals which were detected by the TAGA had never been reported by the companies. Real time proximity monitoring was accomplished by using a triple quadrupole mass spectrometer (TAGA IIe). The TAGA IIe interfaces with a low pressure chemical ionization source that operates at 3 Torr and 100 microA. By monitoring the response of specific parent/daughter ion pairs, the TAGA IIe unit was able to determine the point-of-impingement concentrations of styrene and other ambient airborne chemicals. Positive identification and quantitation of styrene was accomplished by multiple measurements of the following ion pairs: 104/78, 104/77, 104/65 and 104/52. By optimizing various parameters of the TAGA IIe a method detection limit of 1.1 microg/m(3) was achieved for styrene. A daily multi-point calibration was performed using a liquid standard. The maximum half-hour average concentration of styrene measured downwind of the two facilities was 2300 microg/m(3) and the maximum instantaneous level was measured at 15,000 microg/m(3). The mobile capability of the unit was also utilized in determining the ambient airborne concentration of styrene at various distances from the companies with the maximum levels measured at close proximity to the emission sources.


Assuntos
Poluentes Atmosféricos/análise , Monitoramento Ambiental/métodos , Estireno/análise , Indústria Química , Resíduos Industriais , Ontário
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