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1.
Transbound Emerg Dis ; 2020 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-31975551

RESUMO

Scrub typhus is a life-threatening vector-borne disease. During the past decade, the number of areas affected by this disease has expanded in many countries. In this study, we aim to identify the spatiotemporal and demographic characteristics of scrub typhus in Southwest China, an emerging endemic region for scrub typhus. Population-based surveillance data capturing scrub typhus cases in two provinces of Southwest China during 2006-2017 were retrieved. Descriptive temporal and spatial analyses were conducted and stratified by age group. The space-time scan statistic was used to identify spatiotemporal clusters of scrub typhus occurrence at the county level. During the study period, 30,001 scrub typhus cases were recorded in Southwest China, with a total of 61.0% (191/313) of counties being affected; most cases (94.3%) occurred in rural areas. The annual incidence rate increased substantially from 0.25/100,000 in 2006 to 5.38/100,000 in 2017 (>21-fold change). The 0- to 4-year-old and 45- to 64-year-old subgroups had the highest cumulative incidence rates (57.46 and 32.98/100,000, respectively). Furthermore, since 2006, the 0- to 4-year-old (slope = 0.83, p < .01) and 45-64-year-old (slope = 0.69, p < .01) age groups have had the highest increases in incidence of all age groups. The most likely spatial cluster of overall cases (relative risk = 4.13, p < .01) occurred in the southern region of Southwest China and included 41 high-risk counties. In conclusion, scrub typhus appears to be widely distributed and rapidly increasing in Southwest China. Young children and middle-aged adults were the most severely affected groups, and the disease appeared to predominantly cluster in the southern part of Southwest China. Further in-depth surveys to determine the epidemiological characteristics and driving factors of this emerging disease and to facilitate effective control programmes among high-risk groups in the affected areas should be promoted.

2.
Nanoscale ; 12(3): 1448-1454, 2020 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-31829376

RESUMO

Intercalation has proven to be a powerful strategy for physical and chemical property modulation in two dimensional (2D) van der Waals (vdW) materials. Traditional gaseous and chemical intercalation methods offer the ability for mass production, and the electrochemical method provides reversible fine tuning for in situ material investigation. Spatial control, or even direct patterning, of ions is widely required for practical device fabrication and integration; yet it is not realized. Here we demonstrate a self-driven ion (Co2+, Sn4+, and Cu2+) intercalation approach with patterning ability on vdW α-MoO3. It is proved that the self-driven intercalation was enabled by the formation of a local galvanic cell and could be controlled by the metal electrode potential and the solution concentration. The universality of self-intercalation was confirmed in various types of 2D materials (MoS2, WS2, MoSe2, WSe2 and graphene). Furthermore, the feasibility of building heterostructures by multiple species (Sn & Co) intercalation in a single nanosheet was demonstrated for broadband photodetection. The enhancement of conductivity and photoresponse was found to be due to the synergistic effect of lattice distortion from Sn intercalation and the d orbital from the Co atom. This approach offers a feasible way for direct nano-fabrication in 2D vdW material and functional device integration.

3.
PLoS One ; 14(6): e0219110, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31237919

RESUMO

[This corrects the article DOI: 10.1371/journal.pone.0205500.].

4.
PLoS Negl Trop Dis ; 12(11): e0006743, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-30412575

RESUMO

Due to worldwide increased human mobility, air-transportation data and mathematical models have been widely used to measure risks of global dispersal of pathogens. However, the seasonal and interannual risks of pathogens importation and onward transmission from endemic countries have rarely been quantified and validated. We constructed a modelling framework, integrating air travel, epidemiological, demographical, entomological and meteorological data, to measure the seasonal probability of dengue introduction from endemic countries. This framework has been applied retrospectively to elucidate spatiotemporal patterns and increasing seasonal risk of dengue importation from South-East Asia into China via air travel in multiple populations, Chinese travelers and local residents, over a decade of 2005-15. We found that the volume of airline travelers from South-East Asia into China has quadrupled from 2005 to 2015 with Chinese travelers increased rapidly. Following the growth of air traffic, the probability of dengue importation from South-East Asia into China has increased dramatically from 2005 to 2015. This study also revealed seasonal asymmetries of transmission routes: Sri Lanka and Maldives have emerged as origins; neglected cities at central and coastal China have been increasingly vulnerable to dengue importation and onward transmission. Compared to the monthly occurrence of dengue reported in China, our model performed robustly for importation and onward transmission risk estimates. The approach and evidence could facilitate to understand and mitigate the changing seasonal threat of arbovirus from endemic regions.


Assuntos
Dengue/epidemiologia , Dengue/transmissão , Aedes/fisiologia , Aedes/virologia , Animais , Ásia Sudeste , China/epidemiologia , Dengue/virologia , Vírus da Dengue/fisiologia , Humanos , Mosquitos Vetores/fisiologia , Mosquitos Vetores/virologia , Estudos Retrospectivos , Estações do Ano , Viagem
5.
PLoS One ; 13(11): e0205500, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30476930

RESUMO

BACKGROUND: Human brucellosis has become a major public health problem in China. However, the available clinical data on brucellosis cases are limited. METHODS: We retrospectively reviewed medical charts of 2041 patients with confirmed human brucellosis and prospectively recorded their outcomes by telephone interview. These patients were admitted to the Sixth People's Hospital of the Xinjiang Uygur Autonomous region between 1st January and 31st December 2014. Data on these patients were collected from hospital medical records. RESULTS: Many patients presented with fatigue (67%), fever (64%), arthralgia (63%) and sweating (54%). High erythrocyte sedimentation rate (ESR) (69%), high C-reactive protein (CRP) (39%), high alanine aminotransferase (ALT) (33%) and high aspartate aminotransferase (AST) (20%) were the most common laboratory findings, especially in acute patients. There was evidence of focal involvement in 90% of patients. A total of 61.5% of brucellosis patients recovered. Multivariate logistic regression analyses suggested that the risk factors key to unfavorable prognosis were: age≥45 years (OR = 1.75, 95% CI 1.36-2.24), back pain (OR = 1.50, 95% CI 1.16-1.94) and joint tenderness (OR = 1.73, 95% CI 1.13-2.65). The increasing duration of the illness increased the risk of poor prognosis. CONCLUSION: Patients with brucellosis showed different characteristics in different clinical stages. In China, the chronicity rate of human brucellosis is high. The risk of poor prognosis is increased in patients aged 45 years or older, patients who have had brucellosis for a substantial period of time, and patients with back pain or joint tenderness. The clinical management of brucellosis should be improved to include sensitive diagnostic methods for subacute and chronic brucellosis.


Assuntos
Dor nas Costas/fisiopatologia , Brucelose/epidemiologia , Brucelose/fisiopatologia , Prognóstico , Adolescente , Adulto , Idoso , Alanina Transaminase/sangue , Artralgia/fisiopatologia , Aspartato Aminotransferases/sangue , Sedimentação Sanguínea , Brucelose/sangue , Brucelose/microbiologia , Proteína C-Reativa/metabolismo , Criança , Pré-Escolar , China/epidemiologia , Feminino , Humanos , Articulações/fisiopatologia , Masculino , Registros Médicos , Pessoa de Meia-Idade , Fatores de Risco , Adulto Jovem
6.
BMJ Open ; 8(9): e021025, 2018 09 04.
Artigo em Inglês | MEDLINE | ID: mdl-30181184

RESUMO

OBJECTIVE: To investigate the characteristics and predictors for anastomotic leakage after oesophagectomy for oesophageal carcinoma from the perspective of anastomotic level. DESIGN: Retrospective cohort study. SETTINGS: A single tertiary medical centre in China. PARTICIPANTS: From January 2010 to December 2016, all patients with oesophageal cancer of the distal oesophagus or gastro-oesophageal junction undergoing elective oesophagectomy with a curative intent for oesophageal carcinoma with intrathoracic oesophagogastric anastomosis (IOA) versus cervical oesophagogastric anastomosis (COA) were included. We investigated anastomotic level and perioperative confounding factors as potential risk factors for postoperative leakage by univariate and multivariate logistic regression. PRIMARY OUTCOME MEASURES: The primary outcome was the odds of anastomotic leakage by different confounding factors. Secondary outcome was the association of IOA versus COA with other postoperative outcomes. RESULTS: Of 458 patients included, 126 underwent cervical anastomosis and 332 underwent intrathoracic anastomosis. Anastomotic leakage developed in 55 patients (12.0%), with no statistical differences between COA and IOA (16.6% vs 10.2%; p=0.058). Multivariable analysis identified active diabetes mellitus (OR 2.001, p=0.047), surgical procedure (open: reference; minimally invasive: OR 1.770, p=0.049) and anastomotic method (semimechanical: reference; stapled: OR 1.821; handsewn: OR 2.271, p=0.048) rather than anastomotic level (IOA: reference; COA: OR 1.622, p=0.110) were independent predictors of leakage. CONCLUSIONS: Surgical and anastomotic techniques rather than the level of anastomotic site were independent predictors of postoperative anastomotic leakage in patients undergoing oesophageal cancer surgery.


Assuntos
Fístula Anastomótica/etiologia , Carcinoma/cirurgia , Neoplasias Esofágicas/cirurgia , Junção Esofagogástrica/cirurgia , Esôfago/cirurgia , Estômago/cirurgia , Adulto , Idoso , Idoso de 80 Anos ou mais , Anastomose Cirúrgica/efeitos adversos , Anastomose Cirúrgica/métodos , China , Complicações do Diabetes/complicações , Esofagectomia/efeitos adversos , Esofagectomia/métodos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Fatores de Risco , Técnicas de Sutura/efeitos adversos
7.
Cell Death Dis ; 9(3): 386, 2018 03 07.
Artigo em Inglês | MEDLINE | ID: mdl-29515165

RESUMO

Immunomodulatory activity of mesenchymal stem cells (MSCs) is largely mediated by paracrine factors. Our previous studies showed that activation of nuclear factor-kappa B (NF-κB) regulates cytokine/growth factor secretion by MSCs. This study aimed to elucidate the role of Rap1 (repressor/activator protein), a novel modulator involved in the NF-κB pathway, in regulating the immunomodulatory potency of MSCs in acute allograft rejection of heart transplantation. The immunosuppressive potency of wild-type MSCs (WT-MSCs) or Rap1-deficient MSCs (Rap1-/--MSCs) was examined in mice with acute allograft rejection following heart transplantation. With a combination of immunosuppressant rapamycin at a dose of 1 mg/kg/d, WT-MSCs notably prolonged the survival of the transplanted heart compared with Rap1-/--MSCs. Rap1-/--MSCs displayed a marked insensitivity to inhibit the mixed lymphocyte reaction (MLR) due to impaired cytokine production and a significantly reduced activity of NF-κB signaling in vitro. Finally, transplantation of encapsulated WT-MSCs greatly prolonged the survival of the heart allograft compared with encapsulated Rap1-/--MSCs. Our results indicate that Rap1 is essential to maintain the immunomodulatory function of MSCs. Deletion of Rap1 results in impaired immunomodulatory function of MSCs.


Assuntos
Rejeição de Enxerto/metabolismo , Transplante de Coração/efeitos adversos , Células-Tronco Mesenquimais/metabolismo , Proteínas rap1 de Ligação ao GTP/metabolismo , Aloenxertos , Animais , Western Blotting , Proliferação de Células/fisiologia , Células Cultivadas , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Camundongos Knockout , NF-kappa B/metabolismo , Proteínas rap1 de Ligação ao GTP/deficiência , Proteínas rap1 de Ligação ao GTP/genética
8.
Biomed Chromatogr ; 32(3)2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29034541

RESUMO

Uncaria is a multi-source herb and its species identification has become a bottleneck in quality control. To study the identification method of different Uncaria species herbs through HPLC-MS coupled with rDNA Internal Transcribed Spacer (rDNA ITS) sequence, both plant morphological traits and molecular identification were used to determine the species of every collected Uncaria herb. The genetic analysis of different Uncaria species was performed using their rDNA ITS sequence as a molecular marker. Meanwhile, the phylogenetic relationships of 22 samples from six Uncaria species were divided and classified clearly. By optimizing the chromatographic conditions, a practical HPLC method to differentiate various varieties of Uncaria herbs was set up based on a set of characteristic components across each species. A high-performance liquid chromatography-photodiode array detector tandem ion trap and time of flight mass spectrometry technique combined with reference substances was utilized to derive 21 characteristic compounds containing six groups of six Uncaria species in China. Thus, this study provides a feasible method to solve the current problem of confusion in Uncaria species, and makes a significant step forward in the appropriate clinical use, in-depth research and further utilization of different Uncaria species.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , DNA de Plantas/genética , Espectrometria de Massas por Ionização por Electrospray/métodos , Uncaria , DNA Intergênico/genética , DNA de Plantas/análise , Filogenia , Extratos Vegetais/análise , Extratos Vegetais/química , Folhas de Planta/química , Análise de Sequência de DNA , Uncaria/química , Uncaria/classificação , Uncaria/genética
9.
Talanta ; 165: 516-521, 2017 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-28153291

RESUMO

Sensitive glucose detection enables indirect blood glucose sensing through easily accessible biofluids such as saliva and sweat. In this work, silver coated gold nanorods (Au@Ag NRs) were synthesized and used to prepare plasmonic substrate for surface-enhanced Raman spectroscopy (SERS) to leverage highly sensitive detection of glucose for quantitative analysis. By synthetically manipulating of gold NRs and the outer silver shell, the size and aspect ratio of Au@Ag NRs were optimized, and the plasmon resonance wavelength was tuned to approximately the excitation wavelength. 4-Mercaptophenyl-boronic acid (4-MPBA) and 4-Cyanophenylboronic acid (4-CPBA) were used as primary and secondary receptors respectively to specifically capture glucose molecules. The distinct Raman peak at 2226cm-1 of the cyano group in 4-CPBA was used as a signal reporter for glucose sensing. It is located in a biological silent region (1800-2800cm-1), thus offering specific sensing of glucose, without the interference of other endogenous molecules. Our results showed that the SERS substrate was long-term stable. Glucose in urine solution with additive glucose was quantitatively and specifically determined, with the detection limit down to 10-8M. Further experiments using urine from mild diabetes shows positive results, demonstrating the feasibility of clinical use.


Assuntos
Técnicas Biossensoriais/métodos , Diabetes Mellitus/diagnóstico , Glucose/análise , Nanopartículas Metálicas/química , Nanotubos/química , Análise Espectral Raman/métodos , Estudos de Casos e Controles , Diabetes Mellitus/urina , Ouro/química , Humanos , Limite de Detecção , Prata/química
10.
Emerg Infect Dis ; 23(2): 184-194, 2017 02.
Artigo em Inglês | MEDLINE | ID: mdl-28098531

RESUMO

Brucellosis, a zoonotic disease, was made statutorily notifiable in China in 1955. We analyzed the incidence and spatial-temporal distribution of human brucellosis during 1955-2014 in China using notifiable surveillance data: aggregated data for 1955-2003 and individual case data for 2004-2014. A total of 513,034 brucellosis cases were recorded, of which 99.3% were reported in northern China during 1955-2014, and 69.1% (258, 462/374, 141) occurred during February-July in 1990-2014. Incidence remained high during 1955-1978 (interquartile range 0.42-1.0 cases/100,000 residents), then decreased dramatically in 1979-1994. However, brucellosis has reemerged since 1995 (interquartile range 0.11-0.23 in 1995-2003 and 1.48-2.89 in 2004-2014); the historical high occurred in 2014, and the affected area expanded from northern pastureland provinces to the adjacent grassland and agricultural areas, then to southern coastal and southwestern areas. Control strategies in China should be adjusted to account for these changes by adopting a One Health approach.


Assuntos
Brucelose/epidemiologia , Adolescente , Adulto , Distribuição por Idade , Idoso , Idoso de 80 Anos ou mais , Animais , Brucelose/história , Brucelose/microbiologia , Brucelose/transmissão , Criança , Pré-Escolar , China/epidemiologia , Notificação de Doenças , Reservatórios de Doenças , Feminino , Mapeamento Geográfico , História do Século XX , História do Século XXI , Humanos , Incidência , Lactente , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Vigilância da População , Estações do Ano , Análise Espaço-Temporal , Adulto Jovem , Zoonoses/epidemiologia
11.
Infect Dis Poverty ; 6(1): 14, 2017 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-28093076

RESUMO

BACKGROUND: Anthrax is an acute zoonotic infectious disease caused by the bacterium known as Bacillus anthracis. From 26 July to 8 August 2015, an outbreak with 20 suspected cutaneous anthrax cases was reported in Ganquan County, Shaanxi province in China. The genetic source tracking analysis of the anthrax outbreak was performed by molecular epidemiological methods in this study. METHODS: Three molecular typing methods, namely canonical single nucleotide polymorphisms (canSNP), multiple-locus variable-number tandem repeat analysis (MLVA), and single nucleotide repeat (SNR) analysis, were used to investigate the possible source of transmission and identify the genetic relationship among the strains isolated from human cases and diseased animals during the outbreak. RESULTS: Five strains isolated from diseased mules were clustered together with patients' isolates using canSNP typing and MLVA. The causative B. anthracis lineages in this outbreak belonged to the A.Br.001/002 canSNP subgroup and the MLVA15-31 genotype (the 31 genotype in MLVA15 scheme). Because nine isolates from another four provinces in China were clustered together with outbreak-related strains by the canSNP (A.Br.001/002 subgroup) and MLVA15 method (MLVA15-31 genotype), still another SNR analysis (CL10, CL12, CL33, and CL35) was used to source track the outbreak, and the results suggesting that these patients in the anthrax outbreak were probably infected by the same pathogen clone. CONCLUSIONS: It was deduced that the anthrax outbreak occurred in Shaanxi province, China in 2015 was a local occurrence.


Assuntos
Antraz/epidemiologia , Antraz/microbiologia , Bacillus anthracis/genética , Surtos de Doenças , Dermatopatias Bacterianas/epidemiologia , Dermatopatias Bacterianas/microbiologia , Animais , Antraz/transmissão , China/epidemiologia , Feminino , Genótipo , Humanos , Masculino , Análise de Sequência de DNA , Dermatopatias Bacterianas/transmissão , Zoonoses/epidemiologia , Zoonoses/microbiologia , Zoonoses/transmissão
12.
Guang Pu Xue Yu Guang Pu Fen Xi ; 37(2): 350-5, 2017 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-30264960

RESUMO

In integrating lab-on-a-chip (LOC) technologies facilitated with a series of microfluidic units, microfluidic channels, with substrates put into metal nanoparticles, especially when gold, silver or copper nanoparticles, were prepared and pumped into µl or nl analytes. This sample preparation methods have important significance in real time, in-situ trace- or processing reaction analysis jointing with surface enhanced spectroscopies (SES).This combined technologies would integrate the mertis of the two technologies of lab-on-a-chip LOC and SES. LOC has the advantages of minuming the amount of analytes and stable test environments for step by step processing operations to achieve screening samples, segmentating, real-time detecting and so on, whiel SES has the characteristics of fast spectral response, high sensitivemess and selectivness,and in-situ detectoring. On the base of Drude medol and appropriate boundary conditions, external electric field induces localizing plasmon oscillation of valence electron of metal nano particles, then which derivates the mechannisms of resonant localized suface plasmon enhancement and electromagnetic enhancement mechanism of the surface enhanced Raman scattering by dipole polarization. In this paper, combined LOC and localized surface plasmon resonance technologies analysed in biological, pharmaceutical and food safety fileds with additional channels prompting detecting efficiencies and the limits of trace detections further being broken out. This paper also summarizes the application of chip laboratory technology in the fields of public safety testing, biomedical medicine detecting, electrochemical or biological sensors with surface enhanced Raman spectroscopieswhich were capable of high sensitivitiness and molecular spectral fingerprint. LOC technologies have gotten great develoment in their respective fileds, especially combinning with 3D fingerprint technologies, which could precisely control the sizes of 3D structures and high-accuracy manufacture 3D structures according to the special purpose. LSPR and SERS have been more maturing in some applications of near filed imaging and Tip-enhanced Raman spectroscopies (TERS), which have the ability to break through the optical limit of conventional microscopes and do that the width and depth of the SES technologies have been greatly extended in the micro and nano scales. So The jointed technologies would have a bright prospects in the practical applications for the qualitative and semi quantitative determination of trace analysis.

13.
Emerg Infect Dis ; 23(1): 14-21, 2017 01.
Artigo em Inglês | MEDLINE | ID: mdl-27983489

RESUMO

Using national surveillance data for 120,111 human anthrax cases recorded during 1955-2014, we analyzed the temporal, seasonal, geographic, and demographic distribution of this disease in China. After 1978, incidence decreased until 2013, when it reached a low of 0.014 cases/100,000 population. The case-fatality rate, cumulatively 3.6% during the study period, has also decreased since 1990. Cases occurred throughout the year, peaking in August. Geographic distribution decreased overall from west to east, but the cumulative number of affected counties increased during 2005-2014. The disease has shifted from industrial to agricultural workers; 86.7% of cases occurred in farmers and herdsmen. Most (97.7%) reported cases were the cutaneous form. Although progress has been made in reducing incidence, this study highlights areas that need improvement. Adequate laboratory diagnosis is lacking; only 7.6% of cases received laboratory confirmation. Geographic expansion of the disease indicates that livestock control programs will be essential in eradicating anthrax.


Assuntos
Antraz/epidemiologia , Surtos de Doenças , Zoonoses/epidemiologia , Adolescente , Adulto , Idoso , Animais , Antraz/diagnóstico , Antraz/patologia , Bacillus anthracis/patogenicidade , Bacillus anthracis/fisiologia , Criança , Pré-Escolar , China/epidemiologia , Feminino , Humanos , Incidência , Lactente , Recém-Nascido , Gado/microbiologia , Masculino , Pessoa de Meia-Idade , População Rural , População Urbana , Zoonoses/diagnóstico , Zoonoses/patologia
14.
BMC Infect Dis ; 16(1): 760, 2016 12 19.
Artigo em Inglês | MEDLINE | ID: mdl-27993134

RESUMO

BACKGROUND: Brucellosis, one of the world's most important zoonosis, has been re-emerging in China. Shanxi Province, located in northern China, where husbandry development has been accelerated in recent years, has a rather high incidence of human brucellosis but drew little attention from the researchers. This study aimed to describe the changing epidemiology of human brucellosis in Shanxi Province from 2005 to 2014 and explore high-risk towns and space-time clusters for elucidating the necessity of decentralizing disease control resource to township level in epidemic regions, particularly in hotspot areas. METHODS: We extracted data from the Chinese National Notifiable Infectious Disease Reporting System to describe the incidence and spatiotemporal distribution of human brucellosis in Shanxi Province. Geographic information system was used to identify townships at high risk for the disease. Space-Time Scan Statistic was applied to detect the space-time clusters of human brucellosis during the past decade. RESULTS: From 2005 to 2014, a total of 50,002 cases of human brucellosis were recorded in Shanxi, with a male-to-female ratio of 3.9:1. The reported incidence rate increased dramatically from 7.0/100,000 in 2005 to 23.5/100,000 in 2014, with an average annual increase of 14.5%. There were still 33.8% cases delaying diagnosis in 2014. The proportion of the affected towns increased from 31.5% in 2005 to 82.5% in 2014. High-risk towns spread from the north to the center and then south of Shanxi Province, which were basins and adjacent highlands suitable for livestock cultivation. During the past decade, there were 55 space-time clusters of human brucellosis detected in high risk towns; the clusters could happen in any season. Some clusters' location maintained stable over time. CONCLUSIONS: During the last decade, Shanxi province's human brucellosis epidemic had been aggravated and high-risk areas concentrated in some towns located in basins and adjacent highlands. Space-time clusters existed and some located steadily over time. Quite a few cases still missed timely diagnosis. Greater resources should be allocated and decentralized to mitigate the momentum of rise and improve the accessibility of prompt diagnosis treatment in the high-risk townships.


Assuntos
Brucelose/epidemiologia , Adolescente , Adulto , Idoso , Animais , Brucelose/diagnóstico , China/epidemiologia , Diagnóstico Tardio , Notificação de Doenças/estatística & dados numéricos , Epidemias , Feminino , Sistemas de Informação Geográfica , Humanos , Gado , Masculino , Pessoa de Meia-Idade , Fatores de Risco , Estações do Ano , Razão de Masculinidade , Zoonoses/epidemiologia
15.
Int J Clin Exp Pathol ; 8(8): 9342-50, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26464687

RESUMO

OBJECTIVE: To explore the effects of a new management model of comprehensive treatments of acute spinal cord injury (SCI) on clinical application. METHODS: From January 2010 to January 2011, there were 89 patients with acute SCI over the same admission period, including 32 cases divided into the management model group and the other 57 into the control group. Respectively, at the 1, 3 and 6 months after treatment, the score standardization, developed by the American Association of spinal cord injury (ASIA), was used to assess the motor and sensory function during the admission period. At the same time, a follow-up survey was made to investigate the satisfaction of patients and their families. RESULTS: At 1 and 3 months after treatment, the motor and feeling function scores of patients in the experimental group both improved significantly compared with the control group, and the differences were statistically significant (P<0.05). In addition, six months after treatment, the motor and sensory function scores of patients in the control group were not significantly improved any longer; while those scores in the experimental group still significantly recovered, and the difference between experimental and control groups was also statistically significant (P<0.05). According to the follow-up, patients and their families in the experimental group were of greater satisfaction than the control group (P<0.05). CONCLUSIONS: The management model of acute SCI treatment performed perfect clinical effects, and was worth promoting.


Assuntos
Gerenciamento Clínico , Recuperação de Função Fisiológica/fisiologia , Traumatismos da Medula Espinal/terapia , Adaptação Psicológica , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Satisfação do Paciente , Autoeficácia , Traumatismos da Medula Espinal/fisiopatologia , Traumatismos da Medula Espinal/psicologia
16.
BMC Med ; 13: 100, 2015 Apr 28.
Artigo em Inglês | MEDLINE | ID: mdl-25925417

RESUMO

BACKGROUND: Dengue has been a notifiable disease in China since 1 September 1989. Cases have been reported each year during the past 25 years of dramatic socio-economic changes in China, and reached a historical high in 2014. This study describes the changing epidemiology of dengue in China during this period, to identify high-risk areas and seasons and to inform dengue prevention and control activities. METHODS: We describe the incidence and distribution of dengue in mainland China using notifiable surveillance data from 1990-2014, which includes classification of imported and indigenous cases from 2005-2014. RESULTS: From 1990-2014, 69,321 cases of dengue including 11 deaths were reported in mainland China, equating to 2.2 cases per one million residents. The highest number was recorded in 2014 (47,056 cases). The number of provinces affected has increased, from a median of three provinces per year (range: 1 to 5 provinces) during 1990-2000 to a median of 14.5 provinces per year (range: 5 to 26 provinces) during 2001-2014. During 2005-2014, imported cases were reported almost every month and 28 provinces (90.3%) were affected. However, 99.8% of indigenous cases occurred between July and November. The regions reporting indigenous cases have expanded from the coastal provinces of southern China and provinces adjacent to Southeast Asia to the central part of China. Dengue virus serotypes 1, 2, 3, and 4 were all detected from 2009-2014. CONCLUSIONS: In China, the area affected by dengue has expanded since 2000 and the incidence has increased steadily since 2012, for both imported and indigenous dengue. Surveillance and control strategies should be adjusted to account for these changes, and further research should explore the drivers of these trends.


Assuntos
Dengue/epidemiologia , Adulto , China/epidemiologia , Feminino , Humanos , Incidência , Masculino
17.
PLoS One ; 9(11): e113401, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25406082

RESUMO

OBJECTIVE: MicroRNAs (miRNAs) have become the focus of most recent efforts in cancer research. However, there have been inconsistencies in the literature regarding the suitability of circulating miRNAs for early detection of gastrointestinal cancers. This study aims to assess the diagnostic performance of circulating miRNAs in detection of gastrointestinal cancer through a meta-analysis. METHODS: Eligible studies were selected by conducting a systematic literature search of public databases. The sensitivity and specificity were used to plot the summary receiver operator characteristic (SROC) curve and calculate the area under the SROC curve (AUC). The between-study heterogeneity was evaluated by Q test and I2 statistics. Subgroup analyses and meta-regression were further performed to explore the potential sources of heterogeneity. All analyses were performed using the STATA 12.0 software. RESULTS: A total of 107 studies from 42 articles were included for the meta-analysis according to the inclusion criteria. The overall analysis of all gastrointestinal cancers showed that circulating miRNAs have a relatively good diagnostic performance in gastrointestinal cancers, with a sensitivity of 0.75, a specificity of 0.81 and an AUC of 0.85. In addition, subgroup analyses based on different type of miRNA assay suggested that single-miRNA assay displayed a relatively low diagnostic performance with the AUC values of 0.84 for gastric cancer (GC) and 0.79 for colorectal cancer (CRC), while multiple-miRNAs assay significantly improved the diagnosing accuracy with AUC rising to 0.92 for GC and 0.89 for CRC. Another interesting finding was that plasma-based miRNA assay reach a higher accuracy compared with serum-based one for GC, while opposite conclusion was drawn for CRC. CONCLUSIONS: In conclusion, circulating miRNAs, particularly the combination of multiple miRNAs, may present as promising biomarkers for the diagnosis of gastrointestinal cancers. Further large-scale prospective studies are necessary to validate their potential applicability in human cancer diagnosis.


Assuntos
Biomarcadores Tumorais/genética , Neoplasias Gastrointestinais/diagnóstico , Neoplasias Gastrointestinais/genética , MicroRNAs/genética , Biomarcadores Tumorais/sangue , Neoplasias Gastrointestinais/sangue , Humanos , MicroRNAs/sangue , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Sensibilidade e Especificidade
18.
Med Oncol ; 31(11): 225, 2014 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25326859

RESUMO

MicroRNA-182 (miR-182) is overexpressed in several tumors and is found to be associated with adverse clinical characteristics. However, less information on the circulating miR-182 in pancreatic cancer (PCa) is available. The aim of this study was to detect the circulating miR-182 in plasma and to explore its potential diagnostic and prognostic value in PCa. Real-time quantitative PCR was employed to detect circulating miR-182 from 109 PCa and 38 chronic pancreatitis (CP) as well as 50 healthy controls. Our findings revealed that the level of circulating miR-182 in PCa patients was higher than that in CP patients and healthy controls (both at P < 0.05), which was significantly associated with clinical stages (P < 0.001) and lymph node metastasis (P = 0.018). The area under the receiver operating characteristic curve was 0.775, and the optimal cutoff value was 1.63, thus providing a sensitivity of 64.1 % and a specificity of 82.6 %. The diagnosis capability of circulating miR-182 was significantly higher than that of CA19-9, and the combination of two molecules had higher diagnosis capacity (sensitivity of 84.68 % and specificity of 86.77 %). Kaplan-Meier analysis demonstrated that the elevated circulating miR-182 was closely correlated with both shorten overall survival (OS) (P < 0.001) and disease-free survival (DFS) (P < 0.001). Cox analysis indicated that it was an independent prognostic factor for OS and DFS. Our data suggest that circulating miR-182 may be a potential and useful noninvasive tumor marker for diagnosis and prognosis of pancreatic cancer.


Assuntos
Biomarcadores Tumorais/sangue , MicroRNAs/sangue , Neoplasias Pancreáticas/sangue , Neoplasias Pancreáticas/diagnóstico , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Células Neoplásicas Circulantes/metabolismo , Neoplasias Pancreáticas/mortalidade , Prognóstico , Taxa de Sobrevida/tendências
19.
Bioanalysis ; 6(14): 2005-18, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25158969

RESUMO

Allergies occur when a person's immune system mounts an abnormal response with or without IgE to a normally harmless substance called an allergen. The standard skin-prick test introduces suspected allergens into the skin with lancets in order to trigger allergic reactions. This test is annoying and sometimes life threatening. New tools such as lab-on-a-chip and lab-on-a-disc, which rely on microfabrication, are designed for allergy testing. These systems provide benefits such as short analysis times, enhanced sensitivity, simplified procedures, minimal consumption of sample and reagents and low cost. This article gives a summary of these systems. In particular, a cell-based assay detecting both the IgE- and non-IgE-type triggers through the study of degranulation in a centrifugal microfluidic system is highlighted.


Assuntos
Alérgenos/análise , Hipersensibilidade/diagnóstico , Imunoglobulina E/análise , Dispositivos Lab-On-A-Chip , Técnicas Analíticas Microfluídicas/instrumentação , Alérgenos/imunologia , Animais , Teste de Degranulação Basófila/economia , Teste de Degranulação Basófila/instrumentação , Teste de Degranulação Basófila/métodos , Basófilos/imunologia , Basófilos/fisiologia , Degranulação Celular , Desenho de Equipamento , Humanos , Hipersensibilidade/imunologia , Imunoglobulina E/imunologia , Dispositivos Lab-On-A-Chip/economia , Mastócitos/imunologia , Mastócitos/fisiologia , Técnicas Analíticas Microfluídicas/economia , Técnicas Analíticas Microfluídicas/métodos
20.
Lab Chip ; 13(18): 3698-706, 2013 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-23881222

RESUMO

In this paper, we present a lab-in-a-droplet bioassay strategy for a centrifugal microfluidics or lab-on-a-disc (LOAD) platform with three important advancements including density difference pumping, power to disc and bidirectional flow control. First, with the water based bioassay droplets trapped in a micro-channel filled with mineral oil, centrifugal force due to the density difference between the water and oil phases actuates droplet movement while the oil based medium remains stationary. Second, electricity is coupled to the rotating disc through a split-core transformer, thus enabling on-chip real-time heating in selected areas as desired and wireless programmable functionality. Third, an inertial mechanical structure is proposed to achieve bidirectional flow control within the spinning disc. The droplets can move back and forth between two heaters upon changing the rotational speed. Our platform is an essential and versatile solution for bioassays such as those involving DNA amplification, where localized temperature cycling is required. Finally, without the loss of generality, we demonstrate the functionality of our platform by performing real-time polymerase chain reaction (RT-PCR) in a linear microchannel made with PTFE (Teflon) micro-tubing.


Assuntos
Técnicas Analíticas Microfluídicas/instrumentação , Óleo Mineral/química , Centrifugação , DNA/análise , Eletricidade , Dispositivos Lab-On-A-Chip , Reação em Cadeia da Polimerase em Tempo Real/instrumentação , Reação em Cadeia da Polimerase em Tempo Real/métodos , Temperatura Ambiente , Água/química , Tecnologia sem Fio
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