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1.
Ying Yong Sheng Tai Xue Bao ; 31(2): 581-589, 2020 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-32476352

RESUMO

Under the background of rapid urbanization, the degradation of urban river water environment and ecological function in the Taihu Lake Basin has become increasingly prominent. Consequently, it is particularly important to systematically assess urban river habitat. This study referred to and corrected the British Urban River Survey evaluation system. Based on the characte-ristics of urban rivers in the Taihu Lake Basin, we established the evaluation system of stretch habitat quality index (SHQI) of urban river stretches and analyzed the urban river habitat status and spatial variation in the Taihu Lake Basin. The results showed that the SHQI values of the 50 river stretches ranged between 8 and 21, with 3 river stretches classified as "excellent" habitat level, 6 as "good", 27 as "moderate", 9 as "poor", and 5 as "very poor". The vegetation index in urban river habitats in the Taihu Lake Basin was good, whereas that of the physical habitat and material index were bad. The overall ranking of habitats was Zhenjiang: Huzhou: Hangzhou: Jiaxing: Suzhou: Wuxi: Changzhou. There were significant differences in physical habitats, material indices and pollution indices among different cities. The main stream and the tributary section significantly differed in the material index and pollution index, but not significant for SHQI. The evaluation system constructed in this study reflected the current situation of urban river habitats in the Taihu Lake Basin, and could provide guidance for ecological restoration of urban rivers.


Assuntos
Lagos , Rios , China , Cidades , Ecossistema , Monitoramento Ambiental
2.
Artigo em Inglês | MEDLINE | ID: mdl-32419141

RESUMO

BACKGROUND: During ablation for atrial fibrillation (AF), energy delivery toward the left atrial posterior wall may cause esophageal injury (EI). Ablation index (AI) was introduced to estimate ablation lesion size, however, the impact of AI technology on the risk of EI has not been explored. METHOD: From March 2019 to December 2019, 60 patients with paroxysmal AF undergoing first-time ablation were prospectively enrolled. The first 30 consecutive patients were ablated with the AI target value of 400 (AI-400 group), and the later 30 consecutive patients were ablated with the AI target value of 350 at the posterior wall (AI-350 group). Endoscopic ultrasonography was used to evaluate EI postablation. EI was classified as a category 1 (erythema or erosion) or a category 2 (hematoma or ulceration). RESULTS: Compared with the AI-400 group (59.9 ± 8.4 years; male, 60%), the AI-350 group (59.1 ± 9.9 years; male, 50%) had a lower incidence of EI (3.3% vs 26.7%, P = .03). There was no significant difference in the percentage of first-pass PVI between the AI-400 group and the AI-350 group (left PVI: 80% vs 73.4%, P = .54; right PVI: 80% vs 60%, P = .1). Neither ablation time nor fluoroscopy time was significantly different between the AI-400 group and the AI-350 group. CONCLUSIONS: AF ablation guide by AI target value of 350 may reduce esophageal thermal injury and has a similar efficiency on the acute success rate of first-pass PVI compared with an AI target value of 400 at the posterior wall.

3.
Artigo em Inglês | MEDLINE | ID: mdl-32459008

RESUMO

BACKGROUND: Contraction of the esophagus was observed during cryoablation for paroxysmal atrial fibrillation (PAF). The purpose of this study is to investigate the mechanism of esophageal contraction and the correlation between the contraction and esophageal thermal lesions. METHODS: This prospective study enrolled 64 patients with PAF undergoing second-generation cryoballoon (CB2) ablation for pulmonary vein isolation (PVI). During PVI for the left inferior pulmonary vein (LIPV), contrast esophagography was performed before and during cryoablation. The sample population was divided into two; groups A (31 patients) and B (33 patients). Group A was made up of patients in which the distal half of the CB was in proximity to the esophagus, while for group B the esophagus was away from the distal half of the CB. Esophageal contraction was recorded as a variation in the width of the esophageal lumen during PVI. Post-ablation esophageal endoscopy was done on all patients. RESULTS: The reduction in the width of the esophageal lumen in group A was greater than in group B during freezing (40.12±23.24% vs. 8.14±10.35%, P<0.001). Following endoscopy, no apparent esophageal lesion was detected in all patients. CONCLUSION: The extent of esophageal contraction is correlated with the positioning of the esophagus at the distal half of the CB. The findings of this study indicate that esophageal contraction during freezing may be a self-protective mechanism. This article is protected by copyright. All rights reserved.

4.
Europace ; 22(4): 567-575, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32249915

RESUMO

AIMS: The optimal procedural endpoint to achieve permanent pulmonary vein isolation (PVI) during ablation of atrial fibrillation (AF) remains unknown. We aimed to compare the impact of prolonged waiting periods and adenosine triphosphate (ATP) testing after PVI on long-term freedom from AF. METHODS AND RESULTS: In total, 538 patients (median age 61 years, 62% male) undergoing first-time radiofrequency ablation for paroxysmal AF were randomized into four groups: Group 1 [PVI (no testing), n = 121], Group 2 (PVI + 30min waiting phase, n = 151), Group 3 (PVI+ATP, n = 131), and Group 4 (PVI + 30min+ATP, n = 135). The primary endpoint was freedom from AF. Repeat mapping to assess for late pulmonary vein (PV) reconnection was performed in patients who remained AF-free for >3 years (n = 46) and in those who had repeat ablation for AF recurrence (n = 82). During initial procedure, acute PV reconnection was observed in 33%, 26%, and 42% of patients in Groups 2, 3, and 4, respectively. At 36 months, no significant differences in freedom from AF recurrence were observed among all four groups (55%, 61%, 50%, and 62% for Groups 1, 2, 3, and 4, respectively; P = 0.258). Late PV reconnection was commonly observed, with a similar incidence between patients with and without AF recurrence (74% vs. 83%; P = 0.224). CONCLUSION: Although PVI remains the cornerstone for AF ablation, intraprocedural techniques to assess for PV reconnection did not improve long-term success. Patients without AF recurrence after 3 years exhibited similarly high rates of PV reconnection as those that underwent repeat ablation for AF recurrence. The therapeutic mechanisms of AF ablation may not be solely predicated upon durable PVI.

6.
J Cardiovasc Electrophysiol ; 31(2): 401-409, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31828884

RESUMO

INTRODUCTION: Repeat ablation strategy for atrial fibrillation (AF) recurrence after multiple ablation procedures is known to be challenging. This study evaluated the insights of adjunctive ablation for epicardial arrhythmogenic substrates in those patients via a percutaneous epicardial approach. METHODS AND RESULTS: Thirty-five consecutive patients with AF/atrial tachycardia (AT) recurrence, who had two or more prior ablation procedures, were enrolled from September 2016 to December 2018. In addition to a standard endocardial approach, epicardial mapping and ablation were performed via a percutaneous subxiphoid access in the electrophysiology lab. Adjunctive epicardial ablations for left lateral ridge (LLR) were performed in 31 of 35 patients (88.6%) for efficient transmural lesions with pacing capture loss. Marshall Bundle (MB) potentials were documented on epicardial LLR in three patients and abolished by direct epicardial ablation. Bachmann's bundle (BB) was ablated as an epicardial conduction gap in four patients with a refractory anterior wall line. Two epicardial AT/AF triggers were detected followed by successful termination with epicardial ablation. No periprocedural complications occurred. About 23 of 35 patients (65.7%) remained free from AF/AT after 23.2 ± 9 months of the procedure. CONCLUSIONS: Patients with multiple failed prior AF procedures refractory to antiarrhythmic therapy might warrant a percutaneous epicardial mapping and ablation strategy, with adjunctive therapy for targeting LLR/MB, BB, and underlying epicardial triggers in addition to a standard endocardial approach.

8.
Colloids Surf B Biointerfaces ; 184: 110568, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31627101

RESUMO

In this paper, the dispersion performance of biomacromolecule hydrolyzed cellulase from Trichoderma reesei on copper phthalocyanine (CuPc) pigment was first studied. The effect of hydrolysis time, cellulase concentration and environmental pH on the dispersion performance was investigated by particle size distribution and suspension transmittance measurement. The hydrolysis degree of cellulase was determined by FTIR, XRD, UV-vis and fluorescence spectra, potential and particle size analysis, respectively. Subsequently, the hydrolyzed cellulase was combined with sodium dodecyl sulfate (SDS) for acquiring better CuPc suspension based on their synergetic effects on dispersion. The optimal mass ratio of hydrolyzed cellulase to SDS was found to be 1:9. The resulting CuPc dispersion by this hydrolyzed cellulase/SDS composite was characterized by FTIR, TG, TEM, XRD analysis, respectively. This study demonstrated that there were strong interactions between hydrolyzed cellulase and SDS to result in synergistic dispersing effect on CuPc for better stability.


Assuntos
Celulase/química , Corantes/química , Indóis/química , Compostos Organometálicos/química , Dodecilsulfato de Sódio/química , Concentração de Íons de Hidrogênio , Hidrólise , Substâncias Macromoleculares/química , Tamanho da Partícula , Pigmentos Biológicos , Propriedades de Superfície
9.
Artigo em Inglês | MEDLINE | ID: mdl-31350643

RESUMO

PURPOSE: Radiofrequency ablation along the posterior wall of the left atrium may lead to atrioesophageal fistula due to esophageal thermal injury. The purpose of our study was to prospectively investigate whether ablation guided by soluble contrast esophageal visualization (SCEV) reduces injury during atrial fibrillation (AF) ablation. METHODS: Seventy-eight patients with paroxysmal AF undergoing circumferential pulmonary vein isolation (PVI) were randomized to a SCEV group (n = 39) and control group without visualization (n = 39). Cine imaging of the esophagus was performed during soluble contrast swallowing at the beginning of ablation, after adjacent ipsilateral PVI and at the end of the procedure. The ablation lesion set was modified to avoid radiofrequency delivery within the contrast esophagram boundaries. In the control group, a single final ingestion was performed at the end of the procedure. Esophageal injury was assessed by esophagogastroscopy within 24 h in all patients. RESULTS: In the control group, the ablation lesion crossed over the esophagus in 46.2% of patients, whereas in SCEV group, the ablation line violated the boundaries of the esophagus unavoidably in 15.4% of patients (confidence interval (CI); 1.61-13.98, p = 0.003). The incidence of esophageal injury was significantly lower in patients that underwent ablation with SCEV (5.1% vs. 20.5%, CI; 0.04-1.06, p = 0.042). Regardless of randomization group, patients who received ablation which overlapped the esophagus had a higher incidence of esophageal injury compared with those without overlap (37.5 vs. 1.9%, CI; 3.73-271.37, p = 0.000). CONCLUSIONS: Esophageal contrast visualization helps to reduce the potential for esophageal injury during paroxysmal AF ablation. This simple procedural adjunct has important implications to improve safety of paroxysmal AF ablation procedures globally.

10.
Molecules ; 23(5)2018 Apr 27.
Artigo em Inglês | MEDLINE | ID: mdl-29702622

RESUMO

Four new 5-hydroxyanthranilic acid related compounds, named anthocidins A⁻D (1⁻4), two known analogues n-lauryl 5-hydroxyanthranilate (5) and isolauryl 5-hydroxyanthranilate (6), together with benzamide (7), 3-hydroxy-4-methoxycinnamamide (8), and (3S-cis)-hexahydro-3-[(3,4-dihydroxyphenyl)methyl]pyrrolo[1,2-a]pyrazine-1,4-dione (9), were isolated from the fermentation broth of the marine-derived actinomycete, Streptomyces sp. HDa1, which was isolated from the gut of a sea urchin, Anthocidaris crassispina, collected from Hainan Island, China. The structures of these secondary metabolites were elucidated on the basis of their 1D and 2D-NMR and mass spectroscopic data, and anthocidin A was confirmed by single-crystal X-ray diffraction with Cu Kα radiation. Anthocidins A⁻D (1⁻4) feature an acetyl group substitution at the amino group and varying alkyl side chains at the carboxyl group of 5-hydroxyanthranilic acid, and compound 5 was isolated as a natural product for the first time. The cytotoxic and antibacterial activity of compounds 1⁻9 were evaluated.


Assuntos
Actinobacteria/patogenicidade , Antibacterianos/isolamento & purificação , Ouriços-do-Mar/microbiologia , Streptomyces/patogenicidade , ortoaminobenzoatos/isolamento & purificação , Actinobacteria/química , Animais , Antibacterianos/química , Antibacterianos/farmacologia , Linhagem Celular Tumoral , Sobrevivência Celular , China , Cristalografia por Raios X , Fermentação , Modelos Moleculares , Estrutura Molecular , Streptomyces/química , ortoaminobenzoatos/química , ortoaminobenzoatos/farmacologia
11.
Int J Cardiol ; 260: 88-92, 2018 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-29622459

RESUMO

BACKGROUND: Right ventricular pacing (RVP) is associated with an increased incidence of heart failure and may impair cardiac function. Permanent His bundle pacing (HBP) has the potential to physiologically preserve and prevent cardiac dysfunction. This study was to evaluate the feasibility and intermediate follow-up results of upgrade to HBP implantation in patients referred for pulse generator change with long term RVP. METHODS: Twelve of 14 pacing dependent patients who were referred for pulse generator exchange underwent upgrade into HBP successfully in our center. QRS duration, New York Heart Association (NYHA) functional class, echocardiography, use of diuretics and lead parameters were measured at baseline and during the follow-up. RESULTS: Among the 12 patients attempted (mean age, 70.8 ±â€¯8.9 years, 75% males) successfully, the average ejection fraction (EF) was 52.2 ±â€¯11.2%. Nine of 12 patients underwent upgrade to HBP, and three patients with EF < 40% underwent HBP and biventricular pacing (BVP) as well. A significant reduction in mean QRS duration was observed compared with pre-implantation, from 157.8 ±â€¯13.3 ms to 109.3 ±â€¯16.9 ms (p < 0.001). After 6 months follow-up period, median NYHA functional class was improved from 2.7 ±â€¯0.6 to 1.8 ±â€¯0.6 (p = 0.007) and left ventricular internal diastolic diameter (LVIDd) was reduced from 5.5 ±â€¯0.4 cm to 5.3 ±â€¯0.3 cm (p = 0.03). CONCLUSIONS: HBP improves heart failure symptoms with preserved EF by long term RVP. Permanent HBP is feasible and safe for upgrade in patients with long term RVP irrespective of LVEF.


Assuntos
Fibrilação Atrial/diagnóstico por imagem , Fibrilação Atrial/terapia , Fascículo Atrioventricular/diagnóstico por imagem , Fascículo Atrioventricular/fisiologia , Estimulação Cardíaca Artificial/tendências , Idoso , Idoso de 80 Anos ou mais , Fibrilação Atrial/fisiopatologia , Eletrocardiografia/tendências , Estudos de Viabilidade , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Projetos Piloto , Estudos Prospectivos
12.
J Zhejiang Univ Sci B ; 18(11): 946-954, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29119732

RESUMO

BACKGROUND AND OBJECTIVE: Rivaroxaban is a new oral anticoagulant for stroke prevention in patients with non-valvular atrial fibrillation (NVAF), which has less drug-food interaction than warfarin. We conducted this prospective randomized study to evaluate the metabolic benefits as well as the safety and efficacy with rivaroxaban versus warfarin in patients with NVAF following radiofrequency catheter ablation (RFCA). METHODS: From April to July 2014, 60 patients with NVAF undergoing RFCA were prospectively enrolled in our study. Following RFCA, all patients were randomly assigned to receive rivaroxaban (Group R, n=30) or warfarin (Group W, n=30). Metabolic indices including serum total protein, albumin, globulin, and high-density lipoprotein (HDL) as well as bleeding, stroke, and systemic thromboembolism events were evaluated and compared during follow-up after 15, 30, 60, and 90 d of RFCA procedure. RESULTS: Serum total protein, albumin, globulin, and HDL levels were all significantly elevated at each follow-up stage in Group R when compared to the baseline (P<0.05 respectively). In Group W, the metabolic indices decreased at first and then had an increasing trend. There were no deaths or thromboembolic complications in each group. The prevalence of total bleeding complications was similar between Group R and Group W (11/30, 36.7% vs. 10/30, 33.3%, P=0.79). CONCLUSIONS: Patients with NVAF receiving rivaroxaban after RFCA procedures appear to benefit from a metabolic perspective compared with warfarin, providing practical clinical reference for the choice of the anticoagulant. Rivaroxaban seems to be as safe and effective in preventing thromboembolic events as warfarin for these patients.


Assuntos
Anticoagulantes/uso terapêutico , Fibrilação Atrial/tratamento farmacológico , Fibrilação Atrial/metabolismo , Ablação por Cateter , Rivaroxabana/uso terapêutico , Tromboembolia/prevenção & controle , Idoso , Albuminas/análise , Fibrilação Atrial/cirurgia , Ablação por Cateter/efeitos adversos , Feminino , Seguimentos , Globulinas/análise , Hemorragia/prevenção & controle , Humanos , Lipoproteínas HDL/sangue , Masculino , Pessoa de Meia-Idade , Segurança do Paciente , Prevalência , Estudos Prospectivos , Acidente Vascular Cerebral/prevenção & controle , Varfarina/uso terapêutico
13.
Pacing Clin Electrophysiol ; 39(12): 1351-1358, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27723101

RESUMO

BACKGROUND: The mechanisms underlying atrial fibrillation (AF) initiation and pulmonary vein isolation (PVI) effectiveness remain unclear. Ganglionated plexus (GPs) have been implicated in AF initiation and maintenance. In this study, we evaluated the impact of GP ablation in patients with pulmonary vein (PV) firing after PVI. METHODS: Patients with drug-refractory paroxysmal AF undergoing radiofrequency catheter ablation therapy with PVI were screened. Among 840 cases over a 3.75-year period, 12 cases were identified with persistent PV firing (left = 4 and right = 8) after PVI was achieved and left atrial sinus rhythm restored. Adjacent GP ablation was performed anatomically and followed if necessary by additional PV ablation. RESULTS: In eight patients, PV firing was terminated during GP ablation outside of the circumferential ablation line. In one patient, additional PV ablation resulted in cessation of PV firing and in the remaining three patients, firing could not be terminated by GP ablation or additional PVI. CONCLUSION: GP ablation outside of wide antral circumferential line frequently results in the cessation of rapid firing from electrically isolated PVs. These observations suggest that interactions between left atrium and PV beyond electrical conduction warrant consideration in AF mechanisms.


Assuntos
Fibrilação Atrial/cirurgia , Ablação por Cateter/métodos , Gânglios Autônomos/cirurgia , Sistema de Condução Cardíaco/cirurgia , Veias Pulmonares/cirurgia , Cirurgia Assistida por Computador/métodos , Adulto , Idoso , Idoso de 80 Anos ou mais , Pontos de Referência Anatômicos/diagnóstico por imagem , Fibrilação Atrial/diagnóstico por imagem , Mapeamento Potencial de Superfície Corporal/métodos , Feminino , Gânglios Autônomos/diagnóstico por imagem , Sistema de Condução Cardíaco/diagnóstico por imagem , Humanos , Masculino , Pessoa de Meia-Idade , Veias Pulmonares/diagnóstico por imagem , Resultado do Tratamento
14.
Artigo em Inglês | MEDLINE | ID: mdl-27635071

RESUMO

BACKGROUND: The underlying mechanisms of reentry during left posterior fascicular ventricular tachycardia (LPF-VT) remain unclear. The purpose of this study is to describe the components of LPF-VT reentry circuit and their electrophysiological properties. METHODS AND RESULTS: Fourteen consecutive patients with LPF-VT underwent electrophysiology study and radiofrequency ablation. Via a multipolar electrode catheter placed from a retrograde aortic approach, a sharp inflection, high-frequency potential (P1) was detected in 9 patients (64%). The ranges of length and velocity of recorded P1 were 9 to 30 mm and 0.5 to 1.2 mm/ms, respectively. Macroreentry involving the ventricular myocardium was confirmed to be the mechanism in all patients by premature ventricular stimuli delivery or entrainment of LPF-VT with progressive fusion, or both. During LPF-VT, the earliest left posterior fascicle (LPF, P2) was considered to be the site of connection between P1 and P2, and the site of the earliest P2 along the left posterior ventricular septum correlated well with the His-ventricular interval during tachycardia. Radiofrequency ablation focused on the P1 potentials (9 patients with a recorded P1) or earliest P2 (5 patients without a recorded P1) was successful in all 14 patients. After 4.5±3.0 months of follow-up, no patients had recurrence of LPF-VT. CONCLUSIONS: The LPF-VT macroreentrant loop involves the ventricular myocardium, a part of the LPF, a slow conduction zone, and in certain cases, a specially conducting P1 fiber. The His-ventricular interval during LPF-VT correlates with multiple electrophysiological measures and is a useful marker for identification of the optimal ablation site.


Assuntos
Fascículo Atrioventricular/fisiopatologia , Taquicardia Ventricular/fisiopatologia , Adolescente , Adulto , Fascículo Atrioventricular/cirurgia , Ablação por Cateter/métodos , Mapeamento Epicárdico , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Taquicardia Ventricular/cirurgia , Resultado do Tratamento , Septo Interventricular/fisiopatologia , Septo Interventricular/cirurgia
15.
Heart Rhythm ; 13(11): 2151-2158, 2016 11.
Artigo em Inglês | MEDLINE | ID: mdl-27453126

RESUMO

BACKGROUND: Catheter ablation in the right ventricular outflow tract (RVOT) may modify the electrophysiologic substrate for recurrent ventricular tachycardia/ventricular fibrillation (VT/VF) in patients with Brugada syndrome (BrS). OBJECTIVE: The purpose of this study was to investigate the mechanism and arrhythmogenic substrate of VT/VF and to evaluate the long-term outcomes of catheter ablation in patients with BrS. METHODS: Eleven consecutive patients with BrS referred to 2 academic medical centers underwent combined epicardial-endocardial electroanatomic mapping. Catheter ablation was performed in regions of localized conduction slowing. Transmural dispersion of late activation was calculated as the difference between the latest activation between epicardium and endocardium, and low-voltage areas were analyzed. RESULTS: Eleven patients met diagnostic criteria for BrS (spontaneous type 1, n = 9; Na channel provocation = 2). All patients were found to have a localized region in the anterior epicardial RVOT with conduction slowing evidenced by prolonged electrogram duration (78.79 ± 19.87 ms vs 58.93 ± 10.11 ms in epicardial right ventricle, and 59.87 ± 12.61 ms in endocardial RVOT, P <.005, respectively) with variable low voltage (0.97 ± 0.48 mV; median scar area 19.8 ± 25.9 cm2). Epicardial ablation resulted in normalization of spontaneous type 1 Brugada ECG pattern in all patients, and 73% were free from VT/VF at 25 ± 11 months. CONCLUSION: Prolonged electrograms localized to epicardial RVOT with variable low voltage were identified in all patients with BrS. J-point and ST-segment elevation correlated with greater transmural dispersion of late activation and was independent of total low-voltage area. Despite normalization of spontaneous type 1 pattern in all patients after ablation, recurrence was still observed, suggesting the implantable cardioverter-defibrillator as the cornerstone therapy for BrS.


Assuntos
Síndrome de Brugada , Ablação por Cateter , Endocárdio , Mapeamento Epicárdico/métodos , Sistema de Condução Cardíaco , Pericárdio , Adulto , Síndrome de Brugada/complicações , Síndrome de Brugada/diagnóstico , Síndrome de Brugada/fisiopatologia , Síndrome de Brugada/cirurgia , Ablação por Cateter/efeitos adversos , Ablação por Cateter/métodos , China , Eletrocardiografia/métodos , Técnicas Eletrofisiológicas Cardíacas/métodos , Endocárdio/patologia , Endocárdio/fisiopatologia , Feminino , Sistema de Condução Cardíaco/fisiopatologia , Sistema de Condução Cardíaco/cirurgia , Ventrículos do Coração/patologia , Ventrículos do Coração/fisiopatologia , Humanos , Efeitos Adversos de Longa Duração/diagnóstico , Efeitos Adversos de Longa Duração/fisiopatologia , Efeitos Adversos de Longa Duração/prevenção & controle , Masculino , Pessoa de Meia-Idade , Avaliação de Processos e Resultados em Cuidados de Saúde , Pericárdio/patologia , Pericárdio/fisiopatologia , Recidiva , Taquicardia Ventricular/diagnóstico , Taquicardia Ventricular/etiologia , Taquicardia Ventricular/fisiopatologia , Taquicardia Ventricular/prevenção & controle , Fibrilação Ventricular/diagnóstico , Fibrilação Ventricular/etiologia , Fibrilação Ventricular/fisiopatologia , Fibrilação Ventricular/prevenção & controle
16.
J Pharmacol Sci ; 129(4): 205-9, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26627555

RESUMO

Collagen I is the main component of extracellular matrix in cardiac fibrosis. Our previous studies have reported inhibition of farnesylpyrophosphate synthase prevents angiotensin II-induced cardiac fibrosis, while the exact molecular mechanism was still unclear. This paper was designed to investigate the effect of alendronate, a farnesylpyrophosphate synthase inhibitor, on regulating angiotensin II-induced collagen I expression in cultured cardiac fibroblasts and to explore the underlying mechanism. By measuring the mRNA and protein levels of collagen I, we found that alendronate prevented angiotensin II-induced collagen I production in a dose-dependent manner. The inhibitory effect on collagen I expression was reversed by geranylgeraniol, and mimicked by inhibitors of RhoA/Rho kinase pathway including C3 exoenzyme and GGTI-286. Thus we suggested geranylgeranylation-dependent RhoA/Rho kinase activation was involved in alendronate-mediated anti-collagen I synthetic effect. Furthermore, we accessed the activation status of RhoA in alendronate-, geranylgeraniol- and GGTI-286-treated cardiac fibroblasts and gave an indirect evidence for RhoA activation via geranylgeranylation. Then we came to the conclusion that in cardiac fibroblasts, alendronate could protect against angiotensin II-induced collagen I synthesis through inhibition of geranylgeranylation and inactivation of RhoA/Rho kinase signaling. Targeting geranylgeranylation and RhoA/Rho kinase signaling will hopefully serve as therapeutic strategies to reduce fibrosis in heart remodeling.


Assuntos
Alendronato/farmacologia , Angiotensina II/fisiologia , Colágeno Tipo I/biossíntese , Fibroblastos/metabolismo , Miocárdio/citologia , Quinases Associadas a rho/metabolismo , Quinases Associadas a rho/fisiologia , Animais , Células Cultivadas , Depressão Química , Diterpenos/farmacologia , Relação Dose-Resposta a Droga , Ativação Enzimática/efeitos dos fármacos , Fibrose/tratamento farmacológico , Masculino , Terapia de Alvo Molecular , Miocárdio/patologia , Ratos Wistar , Transdução de Sinais/efeitos dos fármacos
17.
J Zhejiang Univ Sci B ; 15(10): 915-22, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25294381

RESUMO

OBJECTIVE: To evaluate the incidence and clinical outcome of adenosine triphosphate (ATP) plus isoproterenol (ISP)-induced non-pulmonary vein (PV) foci before and after circumferential PV isolation (CPVI) during index ablation in patients with paroxysmal atrial fibrillation (PAF). METHODS: In 80 consecutive patients undergoing catheter ablation for drug-refractory, symptomatic PAF at our hospital from April 2010 to January 2011, atrial fibrillation (AF) was provoked with ATP (20 mg) and ISP (20 µg/min) administration before and after CPVI. The spontaneous initiation of AF was mapped and recorded. RESULTS: Before ablation, AF mostly originating from PVs (PV vs. non-PV, 36/70 vs. 3/70; P<0.01) was induced in 39 patients with sinus rhythm. CPVI significantly suppressed AF inducibility; however, more non-PV foci were provoked (post-CPVI vs. pre-CPVI, 13/76 vs. 3/70; P=0.016). Patients with pre- and post-CPVI induced AF (n=49) were divided according to non-PV foci being induced (group N, n=17) or not (group P, n=32). After mean (19.2±8.2) months follow-up, 88.2% (15/17) and 65.6% (21/32) of patients in groups N and P, respectively, were free from AF recurrence (P=0.088). CONCLUSIONS: ATP+ISP administration effectively provokes non-PV foci, especially after CPVI in PAF patients. Although in this study difference did not achieve statistical significance, supplementary ablation targeting non-PV foci might benefit clinical outcome.


Assuntos
Fibrilação Atrial/diagnóstico , Fibrilação Atrial/cirurgia , Ablação por Cateter/métodos , Sistema de Condução Cardíaco/cirurgia , Veias Pulmonares/cirurgia , Trifosfato de Adenosina , Fibrilação Atrial/prevenção & controle , Cardiotônicos , Feminino , Humanos , Incidência , Isoproterenol , Masculino , Recidiva , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Falha de Tratamento , Resultado do Tratamento
18.
Acta Crystallogr Sect E Struct Rep Online ; 66(Pt 2): o259, 2010 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-21579701

RESUMO

The title compound, C(10)H(11)N(3)O(3), was synthesized by the reaction of 3,5-bis-(ethoxy-carbon-yl)-2-formyl-4-methyl-1H-pyrrole and hydrazine hydrate. The angle between the pyrrole ring and the pyridazinone ring is 0.93 (9)°. In the crystal, inter-molecular N-H⋯O and N-H⋯N hydrogen-bond inter-actions link the mol-ecules into a two-dimensional network.

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