Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 815
Filtrar
1.
Food Chem ; 366: 130648, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34325245

RESUMO

A novel label-free fluorescence aptasensor was established for chloramphenicol (CAP) detection by DNA four-arm junction-assisted target recycling and SYBR Green I dye-aided fluorescence-signal amplification. The CAP aptamer was hybridized to its complementary strand (primer) to form a double-stranded primer/aptamer complex. In the presence of CAP, aptamers can specifically bind with CAP to dissociate primers, which can trigger the self-assembly of four hairpins to continuously generate DNA four-arm junctions. After digesting the excess hairpins using T7 exonuclease, SYBR Green I was inserted into the base pair-rich DNA four-arm junctions, which led to a significant increase in fluorescence intensity. Under optimal conditions, the developed aptasensor can detect CAP in a linear range of 1.0 pg mL-1 to 10 ng mL-1 with a detection limit of 0.72 pg mL-1. The recovery rates in milk and honey ranged from 90.3% to 106.6%. Thus, the method shows substantial potential for CAP detection in food products.


Assuntos
Aptâmeros de Nucleotídeos , Técnicas Biossensoriais , Animais , Cloranfenicol/análise , DNA , Limite de Detecção , Leite/química
2.
J Hazard Mater ; 422: 126963, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34449333

RESUMO

Epidemiological evidence regarded the relations of phthalates with children's renal function and its underlying mechanism were largely unknown. We conducted a panel study using 287 paired urine-blood samples by repeated measurements of 103 children (4-13 years) across 3 seasons to explore effects of urinary phthalate metabolites on estimated glomerular filtration rate (eGFR) and the potential role of multiple cytokines. We found that mono-ethyl phthalate (MEP), monobutyl phthalate (MBP), mono-benzyl phthalate (MBzP) and mono-n-octyl phthalate (MOP) were significantly associated with eGFR reduction. Compared with the lowest quartile, MBP, MBzP and MEP in the third and fourth quartiles exhibited a graded decrease in eGFR. Meanwhile, weighted quantile sum regression analyses showed an inverse association of metabolites mixture with eGFR, to which MEP, MBzP, MOP were the major contributors. MEP also remained robust in multiple-phthalate model. Age and weight status might modify such relationships with significant interactions. Furthermore, eGFR related phthalate metabolites were associated with increased multiple cytokines, and CCL27, CXCL1 might be potential mediators between MEP and eGFR with mild mediated proportions. Accordingly, urinary phthalate metabolites were related to eGFR reduction in dose-response manner and multiple cytokines elevation, of which CCL27 and CXCL1 might partly mediate phthalate-associated decreased renal function among children.

3.
Sci Total Environ ; 805: 150284, 2022 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-34537711

RESUMO

Solid-phase extraction (SPE) is a traditional pretreatment procedure widely used for dissolved organic matter (DOM) desalination and enrichment prior to the Fourier transform-ion cyclotron resonance mass spectrometry (FT-ICR MS) analysis, and the extracts are usually acidified to pH = 2 with hydrochloric acid (HCl) before passing through the cartridge. However, little is known about the effects of acidification on the integrity and molecular composition of DOM. Here, the differences in the molecular compositions in acidified and nonacidified DOM samples of soil, seawater and atmospheric aerosol were performed by FT-ICR MS. The results showed that the quantity and intensity of aromatic compounds with high oxygen content (e.g., polyphenols, tannin-like and highly oxygenated organic compounds) were greatly enhanced after acidification, while highly saturated compounds (lipid-like and aliphatic/peptide-like) were absent. The underlying reason is the variation of solubility and hydrolysis of DOM under acidic conditions. Meanwhile, the effect of acidification on the molecular composition of DOM was also dependent on their original environmental media. Based on these results, we suggest that the extracts of soil samples are selectively acidified according to the focus of research, while the extract is acidified for seawater samples and the pH of the extract can be unadjusted for aerosol samples before the SPE procedure. These findings provide a reference for the selection of suitable pretreatment methods for different experimental purposes and for the comprehensive characterization of samples with different properties.


Assuntos
Compostos Orgânicos , Extração em Fase Sólida , Concentração de Íons de Hidrogênio , Espectrometria de Massas , Solo
4.
Front Pharmacol ; 12: 764015, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34744744

RESUMO

Background: Reactive oxygen species (ROS) act as signal mediators to induce tumorigenesis. Objective: This study aims to explore whether chemokine CXCL14 is involved in the proliferation and migration of ROS-induced colorectal cancer (CRC) cells. Methods: The proliferative and migratory capacities of CRC cells treated with or without H2O2 were measured by various methods, including the CKK-8 assay, colony formation assay, flow cytometry, wounding healing assay, and migration assay. Results: The results revealed that H2O2 promoted the proliferation and migration of CRC cells by regulating the cell cycle progression and the epithelial to mesenchymal transition (EMT) process. Furthermore, we noted that the expression level of CXCL14 was elevated in both HCT116 cells and SW620 cells treated with H2O2. An antioxidant N-Acetyl-l-cysteine (NAC) pretreatment could partially suppress the CXCL14 expression in CRC cells treated with H2O2. Next, we constructed CRC cell lines stably expressing CXCL14 (HCT116/CXCL14 and SW620/CXCL14) and CRC cell lines with empty plasmid vectors (HCT116/Control and SW620/Control) separately. We noted that both H2O2 treatment and CXCL14 over-expression could up-regulate the expression levels of cell cycle-related and EMT-related proteins. Moreover, the level of phosphorylated ERK (p-ERK) was markedly higher in HCT116/CXCL14 cells when compared with that in HCT116/Control cells. CXCL14-deficiency significantly inhibited the phosphorylation of ERK compared with control (i.e., scrambled shNCs). H2O2 treatment could partially restore the expression levels of CXCL14 and p-ERK in HCT116/shCXCL14 cells. Conclusion: Our studies thus suggest that aberrant ROS may promote colorectal cancer cell proliferation and migration through an oncogenic CXCL14 signaling pathway.

5.
Cancer Cell Int ; 21(1): 596, 2021 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-34742305

RESUMO

AIM: Previous studies have reported that circular RNA (circRNA) is associated with the pathogenesis of CRC. This study was designed to reveal the mechanism of circ-ring finger protein 121 (circ-RNF121) in colorectal cancer (CRC). MATERIALS AND METHODS: The levels of circ-RNF121, microRNA-1224-5p (miR-1224-5p) and forkhead box M1 (FOXM1) were determined by quantitative real-time polymerase chain reaction (qRT-PCR). Protein level was detected by western blot. Cell proliferation was analyzed by 3-(4,5-Dimethylthazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and cell colony formation assays. Flow cytometry analysis was performed to investigate cell apoptosis. Cell migration and invasion were investigated by transwell and wound-healing assays. Cell glycolysis was detected using glucose, lactate and ADP/ATP ratio assay kits. The binding relationship between miR-1224-5p and circ-RNF121 or FOXM1 was predicted by starBase online database, and identified by dual-luciferase reporter assay. The impacts of circ-RNF121 silencing on tumor formation in vivo were disclosed by in vivo tumor formation assay. KEY FINDINGS: Circ-RNF121 and FOXM1 expression were dramatically upregulated, while miR-1224-5p expression was downregulated in CRC tissues or cells compared with control groups. Circ-RNF121 silencing repressed cell proliferation, migration, invasion and glycolysis but induced cell apoptosis in CRC, which were attenuated by miR-1224-5p inhibitor. Additionally, circ-RNF121 acted as a sponge of miR-1224-5p and miR-1224-5p bound to FOXM1. Circ-RNF121 silencing inhibited tumor growth in vivo. Furthermore, circ-RNF121 was secreted through being packaged into exosomes. SIGNIFICANCE: The finding provided a novel insight into studying circRNA-mediated CRC therapy.

6.
Front Immunol ; 12: 756423, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34733288

RESUMO

Kallistatin or kallikrein-binding protein (KBP) has been reported to regulate angiogenesis, inflammation and tumor progression. Autoimmune uveitis is a common, sight-threatening inflammatory intraocular disease. However, the roles of kallistatin in autoimmunity and autoreactive T cells are poorly investigated. Compared to non-uveitis controls, we found that plasma levels of kallistatin were significantly upregulated in patients with Vogt-Koyanagi-Harada (VKH) disease, one of the non-infectious uveitis. Using an experimental autoimmune uveitis (EAU) model induced by human interphotoreceptor retinoid-binding protein peptide 651-670 (hIRBP651-670), we examined the effects of kallistatin on the pathogenesis of autoimmune diseases. Compared to wild type (WT) mice, kallistatin transgenic (KS) mice developed severe uveitis with dominant Th17 infiltrates in the eye. In addition, the proliferative antigen-specific T cells isolated from KS EAU mice produced increased levels of IL-17A, but not IFN-γ or IL-10 cytokines. Moreover, splenic CD4+ T cells from naïve KS mice expressed higher levels of Il17a mRNA compared to WT naïve mice. Under Th17 polarization conditions, KS mice exhibited enhanced differentiation of naïve CD4+ T cells into Th17 cells compared to WT controls. Together, our results indicate that kallistatin promotes Th17 differentiation and is a key regulator of aggravating autoinflammation in EAU. Targeting kallistatin might be a potential to treat autoimmune disease.

7.
J Genet Genomics ; 2021 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-34757039

RESUMO

Radiotherapy for head and neck cancer can cause serious side effects, including severe damage to the salivary glands, resulting in symptoms such as xerostomia, dental caries, and oral infection. Due to the lack of long-term treatment for the symptoms of xerostomia, current research has focused on finding endogenous stem cells that can differentiate into various cell lineages to replace lost tissue and restore functions. Here, we report that Sox9+ cells can differentiate into various salivary epithelial cell lineages under homeostatic conditions. After ablating Sox9+ cells, the salivary glands of irradiated mice showed more severe phenotypes and the reduced proliferative capacity. Analysis of online single-cell RNA-sequencing data reveals the enrichment of the Wnt/ß-catenin pathway in the Sox9+ cell population. Furthermore, treatment with a Wnt/ß-catenin inhibitor in irradiated mice inhibits the regenerative capability of Sox9+ cells. Finally, we show that Sox9+ cells are capable of forming organoids in vitro and that transplanting these organoids into salivary glands after radiation partially restored salivary gland functions. These results suggest that regenerative therapy targeting Sox9+ cells is a promising approach to treat radiation-induced salivary gland injury.

8.
Nanoscale ; 13(44): 18608-18615, 2021 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-34730599

RESUMO

Pyroptosis is an inflammation-dependent and self-cascade amplifying type of programmed cell death, serving as an effective means for activating the local immune response and improving the anticancer efficacy. As the effector of pyroptosis, gasdermin-E (GSDME) is silenced in most tumor cells. The gene silencing can be reversed by DNA demethylation, but the systemic side effects and toxicity of chemotherapeutic agents are inevitable. In this work, inhaled poly(lactic-co-glycolic acid) (PLGA) porous microspheres loaded with Decitabine (DAC) and Doxorubicin (DOX) (denoted as CO-MPs) were prepared to induce cell pyroptosis for orthotopic lung cancer therapy with fewer systemic side effects. The CO-MPs showed a hollow and porous spherical morphology and exhibited an excellent aerodynamic property, lung distribution and a sustained release effect. The CO-MPs could reverse GSDME silencing and elevate the expression of cleaved-caspase 3 in tumor cells. The cleaved-caspase 3 protein cleaved the GSDEM protein to obtain GSDME-N protein, causing the rupture of cell plasma membranes, release of cell contents and activation of the immune system. The CO-MPs could lead to the suppression of lung tumors, the decrease of the lung metastatic nodules in tumor-bearing mice and the induction of immunological memory that provides continuous protection from the tumor rechallenge. The inhalable microspheres loaded with DAC and DOX could be an effective strategy for lung cancer treatment via the pyroptosis mechanism.


Assuntos
Neoplasias Pulmonares , Piroptose , Animais , Desmetilação do DNA , Doxorrubicina/farmacologia , Neoplasias Pulmonares/tratamento farmacológico , Camundongos , Receptores de Estrogênio
9.
PLoS One ; 16(11): e0259813, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34762701

RESUMO

PURPOSE: Poor sleep quality is closed related with driving accidents. However, due to the lack of a valid instrument for assessing drivers' sleep quality, few studies have examined drivers' sleep quality and its associations with driving behaviours and traffic accidents in China. The aim of this paper is to revise the Sleep Quality Scale (SQS) and assess its reliability and validity in Chinese drivers. METHODS: 522 Chinese drivers aged from 18 to 56 years old agreed to complete the SQS, Daytime Sleepiness Perception Scale version 4 (DSPS-4), Self-report of Risky Driving Behavior (RD-SR) and Self-assessment of the Likelihood of Being Involved in a Risky Driving Situation (RD-SA). RESULTS: The final Chinese version of the SQS contained 23 items across four factors: difficulty in getting up, difficulty in falling asleep, sleep recovery and daytime dysfunction. Second, man scored lower on the difficulty in falling asleep factor but higher on the sleep recovery factor than women. Third, low to moderate correlations were found between the SQS factors and the DSPS-4, RD-SA and RD-SR, indicating that the validity of the revised scale was satisfactory. More importantly, daytime dysfunction factor is an effective predictor of violation involvement and accident involvement. CONCLUSION: The revised SQS has acceptable reliability and validity and can be used as a tool to measure the sleep quality of Chinese drivers.

10.
ACS Appl Mater Interfaces ; 13(47): 56456-56464, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34783524

RESUMO

As a class of functional proteins, enzymes possess inherent insignificant features, for instance, mediocre stability and membrane impermeability and reduced enzymatic activity after modification, which partly limit their biomedical applications. Thus, it is indispensable to exploit robust nanoreactors with high enzymatic activity and good stability and cell permeability. Here, the chiral carbon dots (CDs)-glucose oxidase (GOx) nanoreactors named LGOx and DGOx were constructed by the coassembly of GOx with L/D-CDs, respectively. L/DGOx can significantly enhance the activity of GOx and improve the efficient delivery of GOx to cancer cells. Moreover, these nanoreactors can generate hydrogen peroxide to efficaciously kill cancer cells and restrain tumor growth, and DGOx exhibits higher enzymatic activity than LGOx. According to our understanding, this is the first report about utilizing chiral CDs as vectors to construct effective CDs-enzyme nanohybrids for cancer therapy, which is envisioned to be a versatile strategy for multitudinous biomedical applications.

11.
J Vis Exp ; (177)2021 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-34842236

RESUMO

Laparoscopic transabdominal preperitoneal hernia repair (TAPP) is one of the most widely used methods in inguinal hernia surgery. After the mesh is placed, the peritoneum must be resutured to avoid contact with the tissues and organs in the abdominal cavity. If the peritoneal suture time is too long, the operation and anesthesia time will be prolonged, increasing the burden on the patient. Moreover, improper suture methods cause serious consequences, such as intestinal obstruction and mesh infection. The straight-needle suture method transforms the three-dimensional spatial configuration of the needle holder and the arc needle tip into a two-dimensional planar structure, which greatly reduces the difficulty of suturing. The three-tailed knot can be anchored at the beginning of the suture by its friction and button effect, which has an exact fixation effect. Thus, the suture does not easily slip, and the time to complete the suturing is shortened. Compared with the traditional suture method, the operator can suture the peritoneum more quickly, beginners can pass through the difficult learning curve faster, and skilled operators can also shorten the total operation time of TAPP to a certain extent. Thus, this suture method is extremely amenable to clinical application.

12.
Foods ; 10(11)2021 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-34829169

RESUMO

This paper proposes the combination of headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) and chemometrics as a method to detect the age of Chinese liquor (Baijiu). Headspace conditions were optimized through single-factor optimization experiments. The optimal sample preparation involved diluting Baijiu with saturated brine to 15% alcohol by volume. The sample was equilibrated at 70 °C for 30 min, and then analyzed with 200 µL of headspace gas. A total of 39 Baijiu samples from different vintages (1998-2019) were collected directly from pottery jars and analyzed using HS-GC-IMS. Partial least squares regression (PLSR) analysis was used to establish two discriminant models based on the 212 signal peaks and the 93 identified compounds. Although both models were valid, the model based on the 93 identified compounds discriminated the ages of the samples more accurately according to the goodness of fit value (R2) and the root mean square error of prediction (RMSEP), which were 0.9986 and 0.244, respectively. Nineteen compounds with variable importance for prediction (VIP) scores > 1, including 11 esters, 4 alcohols, and 4 aldehydes, played vital roles in the model established by the 93 identified compounds. Overall, we determined that HS-GC-IMS combined with PLSR could serve as a rapid and accurate method for detecting the age of Baijiu.

13.
Horm Metab Res ; 53(11): 730-737, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34740274

RESUMO

As a selective estrogen receptor modulator (SERM), raloxifene is used in healthy postmenopausal women to prevent bone loss and reduce fractures. However, the benefit of raloxifene is uncertain in the treatment of osteoporosis among patients with end-stage renal disease (ESRD) or those who require maintenance dialysis. We assessed the safety and efficacy of raloxifene in this particular population. Studies were selected from PubMed, Springer, CNKI (Chinese National Knowledge Infrastructure) and Wanfang Database. Randomized controlled trials (RCTs) and prospective studies with control/placebo groups were included. Five studies were included with a total of 244 participants (121 patients in the raloxifene group and 123 patients in the placebo/control group). The median duration of treatment was 12 months. The incidence rate of side effects of raloxifene was 0/121 (0%). There was a significant improvement of lumbar spine bone mineral density (BMD) levels in the raloxifene group compared with the placebo group (MD: 33.88, 95% CI: 10.93, 56.84, p=0.004). There was no significant difference concerning the improvement of femoral neck BMD (MD: 8.42, 95% CI: -10.21, 27.04, p=0.38), intact parathyroid hormone (iPTH) (MD: -12.62, 95% CI: -35.36, 10.13, p=0.28), calcium (MD: -0.08, 95% CI: -0.61, 0.44, p=0.76), phosphorus (MD: 0.18, 95% CI: -0.12, 0.48, p=0.23) or bone alkaline phosphatase (BAP) (MD: -4.33, 95% CI: -14.44, 5.79, p=0.40). Raloxifene seems to be effective in improving the lumbar spine BMD in postmenopausal women with ESRD. More large RCTs are necessary to evaluate the long-term safety of raloxifene in uremic patients.

14.
Am J Cancer Res ; 11(10): 5006-5015, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34765307

RESUMO

Gastric cancer (GC) patients with Epstein-Barr virus (EBV) positivity have demonstrated promising response with immunotherapy. We assessed the efficacy and safety of camrelizumab as salvage treatment in EBV-positive mGC. In this single-arm, phase 2 prospective clinical trial (NCT03755440), stage IV EBV-positive GC patients who failed/could not tolerate previous lines of chemotherapy were given intravenous camrelizumab 200 mg every 2 weeks until disease progression or unacceptable toxicity. The primary endpoint was objective response rate. Secondary endpoints were progression-free survival (PFS), overall survival (OS), disease control rate (DCR), duration of response, and toxicity. Exploratory analysis included the associations between treatment response and tumor mutation burden (TMB), programmed cell death ligand-1 (PD-L1) expression. Six eligible patients were enrolled in the first stage of the study. No patient achieved an objective response; thus, the study did not proceed to the second stage. The DCR was 67% (4/6). The median PFS rate was 2.2 months (95% CI: 1.5-not reached [NR]) and median OS was 6.8 months (95% CI: 1.7-NR). All treatment-related adverse events were grade 1-2, with reactive cutaneous capillary endothelial proliferation (n=4 [67%]) being the most commonly observed event. The only patient with PD-L1 combined positive score >1 had disease progression. Two stable disease and one disease progression were observed in three patients with TMB >10 Mut/Mb. EBV positivity may not be a good predictor for response to camrelizumab in mGC. Newer biomarkers are needed to identify EBV-positive mGC respondents who might benefit from immunotherapy.

15.
Molecules ; 26(22)2021 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-34834003

RESUMO

The detection of trace aroma compounds in samples with complex matrices such as Chinese liquor (Baijiu) requires a combination of several methods, which makes the analysis process very complicated. Therefore, a headspace solid-phase microextraction (HS-SPME) method coupled with two-dimensional gas chromatography time-of-flight mass spectrometry (GC×GC-TOFMS) was developed for the quantitation of a large number of trace compounds in Baijiu. Optimization of extraction conditions via a series of experiments revealed that dilution of the alcohol content of 8 mL of Baijiu to 5%, followed by the addition of 3.0 g of NaCl and subsequent SPME extraction with DVB/CAR/PDMS fiber coating over 45 min at 45 °C was the most suitable. To check the matrix effects, various model Baijiu matrices were investigated in detail. The quantitative method was established through an optimized model synthetic solution, which can identify 119 aroma compounds (esters, alcohols, fatty acids, aldehydes and ketones, furans, pyrazines, sulfur compounds, phenols, terpenes, and lactones) in the Baijiu sample. The developed procedure provided high recovery (86.79-117.94%), good repeatability (relative standard deviation < 9.93%), high linearity (R2 > 0.99), and lower detection limits than reported methods. The method was successfully applied to study the composition of volatile compounds in different types of Baijiu. This research indicated that the optimized HS-SPME-GC×GC-TOFMS method was a valid and accurate procedure for the simultaneous determination of different types of trace compounds in Baijiu. This developed method will allow an improved analysis of other samples with complex matrices.

16.
Foods ; 10(11)2021 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-34829124

RESUMO

Sweetness is an important Baijiu quality marker, but there is limited research on it. In this study, the main contributors to Baijiu sweetness were identified by "sensomics" combined with "flavoromics". A total of 43 volatile compounds (mostly esters) were found that appeared to contribute to Baijiu sweetness, through sensory-guided fractionation and compositional analysis. Correlation analysis between the volatile composition and perceived sweetness of 18 Baijiu samples with different sweet intensities identified 14 potential contributors. Additional testing verified that combining the 14 compounds reproduced Baijiu sweetness exactly, and omission testing identified ethyl hexanoate, hexyl hexanoate and ethyl 3-methylbutanoate as the major contributors to Baijiu sweetness. These findings not only broadened our understanding of Baijiu sweetness, but also highlighted the major contribution of volatile compounds to sweetness perception, knowledge which may facilitate future flavor modification of a wide variety of foods and beverages.

17.
Eur Radiol ; 2021 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-34647177

RESUMO

OBJECTIVES: To evaluate whether the DCE-MRI derived parameters integrated into clinical and conventional imaging variables may improve the prediction of tumor recurrence for locally advanced cervical cancer (LACC) patients following concurrent chemoradiotherapy (CCRT). METHODS: Between March 2014 and November 2019, 79 consecutive LACC patients who underwent pelvic MRI examinations with DCE-MRI sequence before treatment were prospectively enrolled. The primary outcome was disease-free survival (DFS). DCE-MRI derived parameters, conventional imaging, and clinical factors were collected. Univariate and multivariate Cox hazard regression analyses were performed to evaluate these parameters in the prediction of DFS. The independent and prognostic interested variables were combined to build a prediction model compared with the clinical International Federation of Gynecological (FIGO) staging system. RESULTS: Lymph node metastasis (LNM) and the mean value of ve (ve_mean) were independently associated with tumor recurrence (all p < 0.05). The prediction model based on T stage, LNM, and ve_mean demonstrated a moderate predictive capability in identifying LACC patients with a high risk of tumor recurrence; the model was more accurate than the FIGO staging system alone (c-index: 0.735 vs. 0.661) and the combination of ve_mean and the FIGO staging system (c-index: 0.735 vs. 0.688). Moreover, patients were grouped into low-, medial-, and high-risk levels based on the advanced T stage, positive LNM, and ve_mean < 0.361, with which the 2-year DFS was significantly stratified (p < 0.001). CONCLUSIONS: The ve_mean from DCE-MRI could be used as a useful biomarker to predict DFS in LACC patients treated with CCRT as an assistant of LNM and T stage. KEY POINTS: Lower ve_mean is an independent predictor of poor prognosis for disease-free survival in locally advanced cervical cancer patients treated with concurrent chemoradiotherapy (hazard ratio [HR]: 0.016, p<0.023). A combined prediction model based on advanced T stage, LNM, and ve_mean performed better than the FIGO staging system alone.

18.
J Magn Reson Imaging ; 2021 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-34664744

RESUMO

BACKGROUND: The relationship between osteoporosis and intervertebral disc (IVD) degeneration remains controversial. Novel quantitative Dixon (Q-Dixon) and GRAPPATINI T2 mapping techniques have shown potential for evaluating the biochemical components of the spine. PURPOSE: To investigate the correlation of osteoporosis with IVD degeneration in postmenopausal women. STUDY TYPE: Prospective. SUBJECTS: A total of 105 postmenopausal females (mean age, 65 years; mean body mass index, 26 kg/m2 ). FIELD STRENGTH/SEQUENCE: 3 T; sagittal; 6-echo Q-Dixon, multiecho spin-echo GRAPPATINI T2 mapping, turbo spin echo (TSE) T1-weighted and TSE T2-weighted sequences. ASSESSMENT: The subjects were divided into normal (N = 47), osteopenia (N = 28), and osteoporosis (N = 30) groups according to quantitative computed tomography examination. The Pfirrmann grade of each IVD was obtained. Region of interest analysis was performed separately by two radiologists (X.L., with 10 years of experience, and S.C., with 20 years of experience) on a fat fraction map and T2 map to calculate the bone marrow fat fraction (BMFF) from the L1 to L5 vertebrae and the T2 values of each adjacent IVD separately. STATISTICAL TESTS: One-way analysis of variance, post-hoc comparisons, and Kruskal-Wallis H tests were performed to evaluate the differences in the magnetic resonance imaging parameters between the groups. The relationships between BMFF and the IVD features were analyzed using the Spearman correlation analysis and linear regression models. RESULTS: There were significant differences in BMFF among the three groups. The osteoporosis group had higher BMFF values (64.5 ± 5.9%). No significant correlation was found between BMFF and Pfirrmann grade (r = 0.251, P = 0.06). BMFF was significantly negatively correlated with the T2 of the adjacent IVD from L1 to L3 (r = -0.731; r = -0.637; r = -0.547), while significant weak correlations were found at the L4 to L5 levels (r = -0.337; r = -0.278). DATA CONCLUSION: This study demonstrated that osteoporosis is associated with IVD degeneration. LEVEL OF EVIDENCE: 2 TECHNICAL EFFICACY: Stage 4.

19.
Artigo em Inglês | MEDLINE | ID: mdl-34705658

RESUMO

Seeking good correspondences between two images is a fundamental and challenging problem in the remote sensing (RS) community, and it is a critical prerequisite in a wide range of feature-based visual tasks. In this article, we propose a flexible and general deep state learning network for both rigid and nonrigid feature matching, which provides a mechanism to change the state of matches into latent canonical forms, thereby weakening the degree of randomness in matching patterns. Different from the current conventional strategies (i.e., imposing a global geometric constraint or designing additional handcrafted descriptor), the proposed StateNet is designed to perform alternating two steps: 1) recalibrates matchwise feature responses in the spatial domain and 2) leverages the spatially local correlation across two sets of feature points for transformation update. For this purpose, our network contains two novel operations: adaptive dual-aggregation convolution (ADAConv) and point rendering layer (PRL). These two operations are differentiable, so our network can be inserted into the existing classification architecture to reduce the cost of establishing reliable correspondences. To demonstrate the robustness and universality of our approach, extensive experiments on various real image pairs for feature matching are conducted. Experiments reveal the superiority of our StateNet significantly over the state-of-the-art alternatives.

20.
Opt Express ; 29(17): 26701-26712, 2021 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-34615099

RESUMO

Interferometric Rayleigh scattering technique is commonly employed to measure single-point velocity fluctuation and its standard deviation in a high-speed flow due to many benefits, such as high accuracy, easy data interpretation, and high sampling rate. However, this technique suffers from a severe problem often referred to as the weak Rayleigh scattering signal, especially in the supersonic and hypersonic flow with an extremely low gas molecule density. An asymmetry cavity structure that could cost-effectively improve the Rayleigh scattering (RS) signal of interest is designed and used in the interferemetric Rayleigh scattering technique. The ZEMAX simulations suggest that the parallel beam can be repeatedly reflected in the resonant cavity and can be focused in a measurement region with the order of 0.67 mm×1.31 mm. The number of propagating rays inside the cavity can reach about 50. The fidelity of this proposed cavity is then verified by the Rayleigh scattering imaging experiments. Results show that this cavity allows the laser beam to reflect several times in the resonant cavity, and the RS signal intensity in the major axis can be 10.4 times larger than that of the incident laser. The cavity is finally employed under realistic supersonic flow velocity measurements, where the results conclusively illustrate that the Rayleigh scattering signal of interest in a single direction can be improved by a factor of 4∼5. In addition, the bi-directional (both the axial and radial directions) velocity parameters can also be obtained simultaneously. The axial velocity and its standard deviation are similar to conventional single-line ones.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...