Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 14 de 14
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Zhongguo Zhong Yao Za Zhi ; 44(19): 4095-4100, 2019 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-31872682

RESUMO

The study is aimed to effectively obtain the planting area of traditional Chinese medicine resources. The herbs used as the material for traditional Chinese medicine are mostly planted in natural environment suitable mountainous areas. The UAV low altitude remote sensing data were used as the samples and the GF-2 remote sensing images were applied for the data source to extract the planting area of Salvia miltiorrhiza and Artemisia argyi in Luoning county combined with field investigation. Remote sensing satellite data of standard processing obtain specific remote sensing data coverage. The UAV data were pre-processed to visually interpret the species and distribution of traditional Chinese medicine resources in the sample quadrat. Support vector machine( SVM) was used to classify and estimate the area of traditional Chinese medicine resources in Luoning county,confusion matrix was used to determine the accuracy of spatial distribution of traditional Chinese medicine resources. The result showed that the application of UAV of low altitude remote sensing technology and remote sensing image of satellite in the extraction of S. miltiorrhiza and other varieties planting area was feasible,it also provides a scientific reference for poverty alleviation policies of the traditional Chinese medicine Industry in local areas.Meanwhile,research on remote sensing classification of Chinese medicinal materials based on multi-source and multi-phase high-resolution remote sensing images is actively carried out to explore more effective methods for information extraction of Chinese medicinal materials.


Assuntos
Altitude , Medicamentos de Ervas Chinesas , Medicina Tradicional Chinesa , Tecnologia de Sensoriamento Remoto , Recursos Naturais , Máquina de Vetores de Suporte
2.
Molecules ; 24(15)2019 Jul 27.
Artigo em Inglês | MEDLINE | ID: mdl-31357638

RESUMO

To obtain diterpene glycosides from an aqueous extract of the aerial parts of Isodon henryi and further investigate their cytotoxicities, in this study, a total of seven compounds were isolated, including six ent-kaurane diterpene glycosides (1-6) and one diterpene aglycon (7). Among the seven ent-kaurane diterpenes obtained, four were novel compounds, including ent-7,20-epoxy- kaur-16-en-1α,6ß,7ß,15ß-tetrahydroxyl-11-O-ß-d-glucopyranoside (1), ent-7,20-epoxy-kaur-16-en- 6ß,7ß,14ß,15ß-tetrahydroxyl-1-O-ß-d-glucopyranoside (2), ent-7,20-epoxy-kaur-16-en-6ß,7ß,15ß- trihydroxyl-1-O-ß-d-glucopyranoside (3), and ent-7,20-epoxy-kaur-16-en-7ß,11ß,14α,15ß-tetrahydr- oxyl-6-O-ß-d-glucopyranoside (4), and three were isolated from this plant for the first time (5-7). Their structures were elucidated by utilizing spectroscopic methods and electronic circular dichroism analyses. Furthermore, the cytotoxicities of all seven compounds were investigated in four human cancer cell lines, including A2780, BGC-823, HCT-116, and HepG2. The IC50 values of these diterpenes ranged from 0.18 to 2.44 mM in the tested cell lines. In addition, the structure-cytotoxicity relationship of diterpene glycosides was also evaluated to study the effect of glycosylation on the cytotoxicity of diterpene compounds.


Assuntos
Diterpenos de Caurano/química , Diterpenos de Caurano/farmacologia , Glicosídeos/química , Glicosídeos/farmacologia , Isodon/química , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/farmacologia , Modelos Moleculares , Estrutura Molecular , Análise Espectral , Relação Estrutura-Atividade
3.
Biomed Pharmacother ; 115: 108930, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31055234

RESUMO

Backgroud Icariin, a major bioactive pharmaceutical component of the Chinese herbal medicine Epimedii Herba, has demonstrated lipid-lowering and anti-obesity effects. Irisin/ fibronectin type III domain-containing 5 (FNDC5) protects against obesity by inducing browning in white adipose tissue. Objectives This study investigated the effects of icariin on irisin/FNDC5 expression in C2C12 myotubes. Method Cultured murine C2C12 myocytes were used to study the effects of icariin on irisin/FNDC5 expressions by Western-blot, qPCR, Elisa and Immunofluorescence. We also investigated FNDC5 expression in icariin-treated intact mice. Results Icariin increased irisin/FNDC5 protein levels. mRNA levels of irisin/FNDC5 were also increased in C2C12 myocytes after treatment with icariin. Icariin increased peroxisome proliferator-activated receptor gamma co-activator 1alpha (PGC-1α) protein and mRNA levels. Additionally, icariin exposure resulted in phosphorylation of AMP-activated protein kinase (AMPK) in a dose-dependent manner. The regulatory effect of icariin on FNDC5 protein expression was blocked by the AMPK antagonist compound C or silencing of AMPK, suggesting that icariin increased FNDC5 protein expression via the AMPK pathway. In vivo, icariin decreased body weight gain in C57BL/6 mice and increased FNDC5, PGC-1α, and p-AMPK expression levels in skeletal muscle. Conclusions Taken together, our results indicated that icariin induces irisin/FNDC5 expression via the AMPK pathway, indicating that icariin may be promising as an anti-obesity drug.


Assuntos
Proteínas Quinases Ativadas por AMP/metabolismo , Fibronectinas/genética , Flavonoides/farmacologia , Fibras Musculares Esqueléticas/efeitos dos fármacos , Animais , Técnicas de Cultura de Células , Linhagem Celular , Relação Dose-Resposta a Droga , Medicamentos de Ervas Chinesas , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Fibras Musculares Esqueléticas/metabolismo , Músculo Esquelético/efeitos dos fármacos , Músculo Esquelético/metabolismo , Coativador 1-alfa do Receptor gama Ativado por Proliferador de Peroxissomo/genética , Regulação para Cima
4.
Zhongguo Zhong Yao Za Zhi ; 44(2): 319-323, 2019 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-30989952

RESUMO

The chemical constituents of the water extraction of the aerial parts of Isodon henryi were investigated by various chromatographic methods including D-101 macroporous adsorptive resins,silica gel,sephadex LH-20,and semi-preparative HPLC. As a result,ten compounds were separated and purified. By analyses of the UV,IR,MS,NMR spectra,their structures were determined as rabdosinate( 1),lasiokaurin( 2),epinodosinol( 3),rabdosichuanin C( 4),epinodosin( 5),hebeirubescensin k( 6),rubescensin C( 7),enmenol( 8),oridonin( 9),and enmenol-1-ß-glucoside( 10). Compounds 1-8 and 10 were isolated from I. henryi for the first time. Compounds 2 and 9 showed inhibitory effects against four tumor cells,with IC50 values of 2. 25-9. 32 µmol·L-1.


Assuntos
Isodon/química , Compostos Fitoquímicos/análise , Extratos Vegetais/química , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Componentes Aéreos da Planta/química
5.
Nat Prod Res ; 32(20): 2424-2430, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-29320879

RESUMO

Cytotoxic diterpenoids were enriched and orientation prepared from the aerial parts of Isodon excisoides target-guided by UPLC-LTQ-Orbitrap-MS. Four diterpenoids were obtained, including a novel compound: 1α-acetoxy-7α, 14ß, 20α-trihydroxy-ent-kaur-16-en-15-one (1); together with three known compounds kamebakaurin (2), lasiokaurin (3), enmenol-1-ß-glucoside (4). Their structures were elucidated on the basis of spectroscopic methods in conjunction with published data for their analogues. All compounds were tested for their cytotoxic effects against five human cancer cell lines HCT-116, HepG2, A2780, NCI-H1650 and BGC-823, respectively. Compounds 1 and 2 showed obviously cytotoxic activity against the five cancer cell lines with IC50 ranging from 1.06 to 3.60 µM. Compounds 3 and 4 showed selective cytotoxic activity.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Diterpenos/farmacologia , Isodon/química , Antineoplásicos Fitogênicos/isolamento & purificação , Linhagem Celular Tumoral , China , Diterpenos/isolamento & purificação , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Estrutura Molecular , Componentes Aéreos da Planta/química
6.
Zhongguo Zhong Yao Za Zhi ; 42(16): 3078-3083, 2017 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-29171224

RESUMO

Through planting regionalization the scientific basis for planting area of high-quality medicinal materials was predicted. Through interview investigation and field survey, the distribution information of Corni Fructus in China was collected,and 89 sampling point from 14 producing areas were collected. Climate and topography of Corni Fructus were analyzed, the ecological adaptability of study was conducted based on ArcGIS and Maxent. Different suitability grade at potential areas and regionalization map were formulated. There are nine ecological factors affecting the growth of Corni Fructus, for example precipitation in November and March and vegetation type. The results showed that the most suitable habitats are Henan, Shaanxi, Zhejiang, Chongqing, Hubei, Sichuan, Anhui, Hunan and Shandong province. Using the spatial analysis method,the study not only illustrates the most suitable for the surroundings of Corni Fructus,but also provides a scientific reference for wild resource tending, introduction and cultivation, and artificial planting base and directing production layout.


Assuntos
Cornus/crescimento & desenvolvimento , Plantas Medicinais/crescimento & desenvolvimento , China , Clima , Ecologia , Sistemas de Informação Geográfica , Solo
7.
Metab Syndr Relat Disord ; 15(9): 450-457, 2017 11.
Artigo em Inglês | MEDLINE | ID: mdl-28934021

RESUMO

BACKGROUND: Perivascular adipose tissue (PVAT) can regulate vascular homeostasis by secreting various adipokines. This study investigated the effects of PVAT browning on its endocrine function. METHODS: In the first section of our study, male Sprague-Dawley rats were randomly divided into cold exposure (8°C) and 24°C acclimation groups. After cold exposure for 7 days, interscapular brown adipose tissue (iBAT), subcutaneous white adipose tissue, thoracic aortic PVAT, and abdominal aortic PVAT (aPVAT) were harvested for histological and brown marker gene expression analysis. In the second part, male rats were fed a high fat diet (HFD) for 10 weeks. In the 11th week, the rats were treated with or without cold exposure. After 14-day cold exposure, aPVAT was collected for histological, gene, and protein expression analysis. RESULTS: Cold exposure had a browning effect on aPVAT by increasing UCP-1 and PGC-1α expression levels. After HFD feeding for 10 weeks, 14-day cold exposure was still able to induce aPVAT browning. Compared with thermoneutrality acclimation rats, TNF-α, IL-6, and p-p65 expression levels were significantly lower in aPVAT from HFD-fed rats with cold exposure. In contrast, p-AMPK expression levels were increased in aPVAT from HFD-fed rats with cold exposure. CONCLUSIONS: Our study demonstrated that browning of aPVAT in HFD-fed rats lowered the pro-inflammatory adipokine expression levels and activated AMPK.


Assuntos
Gordura Abdominal/fisiologia , Tecido Adiposo Marrom/fisiologia , Tecido Adiposo Branco/fisiologia , Transdiferenciação Celular/fisiologia , Temperatura Baixa , Inflamação/prevenção & controle , Paniculite/prevenção & controle , Adipocinas/metabolismo , Animais , Aorta Abdominal , Masculino , Ratos , Ratos Sprague-Dawley
8.
J Liq Chromatogr Relat Technol ; 39(8): 422-427, 2016 May 08.
Artigo em Inglês | MEDLINE | ID: mdl-27366022

RESUMO

Establish a quantitative analysis of multi-components by the single marker (QAMS) method for quality evaluation and validate its feasibilities by the simultaneous quantitative assay of four main components in Linderae Reflexae Radix. Four main components of pinostrobin, pinosylvin, pinocembrin, and 3,5-dihydroxy-2-(1-p-mentheneyl)-trans-stilbene were selected as analytes to evaluate the quality by RP-HPLC coupled with a UV-detector. The method was evaluated by a comparison of the quantitative results between the external standard method and QAMS with a different HPLC system. The results showed that no significant differences were found in the quantitative results of the four contents of Linderae Reflexae Radix determined by the external standard method and QAMS (RSD <3%). The contents of four analytes (pinosylvin, pinocembrin, pinostrobin, and Reflexanbene I) in Linderae Reflexae Radix were determined by the single marker of pinosylvin. This fingerprint was the spectra determined by Shimadzu LC-20AT and Waters e2695 HPLC that were equipped with three different columns.

9.
Zhongguo Zhong Yao Za Zhi ; 41(18): 3361-3365, 2016 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-28925118

RESUMO

The chemical constituents of the water extraction of the aerial parts of Isodon excisoides were investigated by various chromatographic methods including D-101 macroporous adsorptive resins, silica gel, Sephadex LH-20, MCI and semi-preparative HPLC. As a result, six compounds were separated and purified.By analyses of the HR-ESI-MS, 1D and 2D NMR spectra, their structures were determined as 3-O-ß-D-allopyranosyl-1-octen-3-ol(1), blumenolA (2), lumichrome (3), loliolide(4), cirsiliol(5) and pedalitin(6). Compound 1 was a new compound, and compounds 2-4 were isolated from this plant for the first time.


Assuntos
Isodon/química , Compostos Fitoquímicos/química , Componentes Aéreos da Planta/química , Estrutura Molecular
10.
Zhong Yao Cai ; 37(4): 587-9, 2014 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-25345130

RESUMO

OBJECTIVE: To compare and analyze the wild and cultivated Lonicerae Japonicae Flos from Xinmi. METHODS: Macroscopic identification and microscopic identification were adopted to compare and analyze characteristics of the wild and cultivated Lonicerae Japonicae Flos from Xinmi. The content of chlorogenic acid and luteoloside were determined by the methods in the Chinese Pharmacopoeia. DNA molecular marker technology was used to identify Lonicerae Japonicae Flos of different varieties. RESULTS: The differences in macroscopic and microscopic characteristics as well as the content of chlorogenic acid and luteoloside between them had been found. PCR amplification reaction system and procedure of Lonicerae Japonicae Flos from Xinmi were optimized. An effective primer for identification was chosen from 11 primers. CONCLUSION: The results provide the scientific basis for quality assessment of Lonicerae Japonicae Flos from Xinmi.


Assuntos
DNA Espaçador Ribossômico/genética , Flores/anatomia & histologia , Lonicera/anatomia & histologia , Lonicera/genética , Ácido Clorogênico/análise , Cromatografia Líquida de Alta Pressão , Primers do DNA/genética , DNA de Plantas/genética , Flores/classificação , Flores/genética , Lonicera/classificação , Microscopia Eletrônica , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Controle de Qualidade , Análise de Sequência de DNA
11.
Zhong Yao Cai ; 36(1): 6-9, 2013 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-23750401

RESUMO

OBJECTIVE: To study the classification and genetic relationship among difference cultivated varieties of Lonicera japonica cultivated in Xinmi. METHODS: Observed the biological characteristics of Lonicera japonica cultivated in Xinmi and visited farmers; Genetic diversity was analyzed by RAPD molecular biology techniques. RESULTS: Classified the cultivated varieties and set up an index key. RAPD fingerprint was established, 10 primers were chosen. A total of 60 DNA fragments, among which 50 bands was polymorphic, accounting for 83.33% which indicated the genetic diversity of the samples was good. CONCLUSION: Genetic relationship among different cultivated varieties are determined which provide scientific basis for resources protection, development of Lonicera japonica cultivated in Xinmi and its quality evaluation.


Assuntos
Variação Genética , Lonicera/classificação , Lonicera/genética , Técnica de Amplificação ao Acaso de DNA Polimórfico/métodos , China , Análise por Conglomerados , Primers do DNA/genética , DNA de Plantas/genética , Flores/anatomia & histologia , Flores/genética , Lonicera/anatomia & histologia , Filogenia , Folhas de Planta/anatomia & histologia , Folhas de Planta/genética , Polimorfismo Genético
12.
Zhong Yao Cai ; 34(8): 1167-72, 2011 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-22233028

RESUMO

OBJECTIVE: To analyze the correlation between the quality of Cornus officinalis and the contents of the inorganic elements in the planting soil. METHODS: The contents of 11 kinds of inorganic elements including Zn, Mn, K, Fe, Mg, Ca, Cu, Cd, As, Pb and Hg in Cornus officinalis and the planting soil were determined; The contents of loganin, ursolic acid, polysaccharides, tannins, organic acid, water-soluble extractives and ethanol-soluble extractives in Cornus officinalis were determined; The correlation between the quality of Cornus officinalis and the contents of the inorganic elements in the planting soil was analyzed. RESULTS: There was no significant difference in the content of chemical components of Cornus officinalis produced in the three main areas. The contents of loganin and water-soluble extract complied with pharmacopoeial provisions. Especially, inorganic elements of Fe, Mn and K had significant effects on the quality of Cornus officinalis. CONCLUSION: The results provide the foundation for the selection of cultivating area and the reference for standard cultivation of Cornus officinalis.


Assuntos
Cornus/química , Frutas/química , Plantas Medicinais/química , Solo/análise , Oligoelementos/análise , China , Cornus/crescimento & desenvolvimento , Iridoides/análise , Ferro/análise , Manganês/análise , Plantas Medicinais/crescimento & desenvolvimento , Polissacarídeos/análise , Potássio/análise , Controle de Qualidade , Solo/química , Espectrofotometria Ultravioleta , Triterpenos/análise
13.
Zhong Yao Cai ; 33(12): 1861-5, 2010 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-21548360

RESUMO

OBJECTIVE: To distinguish Magnolia biondii of ten different cultivated. METHODS: The original species can be identified by the morphological characteristics and microscopic characteristics. The volatile oil is determinated by the method of Chinese pharmacopoeia. RESULTS: The ten cultivated Mangolia biondii have obviously differents in morphological characteristics, microscopic characteristics and volatile oil. CONCLUSION: It is important for development application of Magnolia biondii and variety improvement.


Assuntos
Flores/anatomia & histologia , Magnolia/anatomia & histologia , Óleos Voláteis/análise , Plantas Medicinais/anatomia & histologia , Flores/química , Magnolia/química , Magnolia/classificação , Magnolia/crescimento & desenvolvimento , Óleos Voláteis/isolamento & purificação , Folhas de Planta/anatomia & histologia , Folhas de Planta/química , Caules de Planta/anatomia & histologia , Caules de Planta/química , Plantas Medicinais/química , Plantas Medicinais/classificação , Plantas Medicinais/crescimento & desenvolvimento , Pós , Controle de Qualidade
14.
Yao Xue Xue Bao ; 43(2): 173-80, 2008 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-18507345

RESUMO

To separate and identify the chemical constituents from the leaves of Broussonetia papyrifera (Linn.) Vent, various columns including Diaion HP-20, Toyopearl HW-40C, Sephadex LH-20, silica gel were employed for the isolation and purification of compounds from the leaves of B. papyrifera. The structures of the compounds were elucidated by their physiochemical characteristics and spectral data. Nineteen compounds were isolated from the leaves of B. papyrifera and their structures were identified as apigenin (1), apigenin-7-O-beta-D-glucopyranoside (2), chrysoerid-7-O-beta-D-glucopyranoside (3), apigenin-7-O-beta-D-glucopyranuronide (4), vitexin-7-O-beta-D-glucopyranoside (5), luteolin (6), 5,7,4'-trihydroxyl-6-C-[a-L-rhamnopyranosyl (1-->2)]-beta-D-glucopyranosyl flavone (7), 5,7,4'-trihydroxyl-8-C-[a-L-rhamnopyranosyl (1-->2)]-beta-D-glucopyranosyl flavone (8), saponaretin (9), vitexin (10), benzyl benzoate-2, 6-di-O-beta-D-glucopyranoside (11), (2R, 3R, 5R, 6S, 9R)-3-hydroxy-5,6-epoxy-beta-ionol-2-O-beta-D-glucopyranoside (12), (2R, 3R, 5R, 6S, 9R)-3-hydroxyl-5,6-epoxy-acetyl-beta-ionol-2-O-beta-D-glucopyranoside (13), ficustriol (14), (6S, 9S)-roseoside (15), 3beta-hydroxy-5alpha,6alpha-epoxy-beta-ionone-2alpha-O-beta-D-glucopyranoside (16), icariside B1 (17), sammangaoside A (18), 3-hydroxy-5alpha,6alpha-epoxy-beta-ionone (19). Compounds 11, 12 and 13 are new compounds, the others are isolated from this genus Broussonetia for the first time.


Assuntos
Broussonetia/química , Glucosídeos/isolamento & purificação , Apigenina/química , Apigenina/isolamento & purificação , Glucosídeos/química , Luteolina/química , Luteolina/isolamento & purificação , Estrutura Molecular , Folhas de Planta/química , Plantas Medicinais/química
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA