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1.
Water Res ; 226: 119274, 2022 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-36332296

RESUMO

With the development of comprehensive utilization of high-salinity wastewater, salt resources regeneration has been considered as the fundamental requirement for process sustainability and economic benefits. As one of the potential candidates, bipolar membrane electrodialysis (BMED) was rapidly developed in recent years for the treatment of saline wastewater. Different from other methods directly obtaining salts or condensed wastewater, BMED could utilize and convert the dissolved waste salt into higher-value acid and alkali simultaneously, which has various advantages including outstanding environmental effects and economic benefits. In this review, the recent applications of BMED for waste salt recovery and high-value acid/alkali generation from saline wastewater were systematically outlined. Based on the summary above, the economy analysis of BMED was further reviewed from the roles of desalination and resources recovery. In addition, the BMED-based processes integrated with in-situ utilization of the generated acid/alkali resources were discussed. Furthermore, the influence of operating factors on BMED performance were outlined. Finally, the strategies for improving BMED performance were concluded. Furthermore, the future application and prospects of BMED was presented. This work would provide guidance for the applications of bipolar membrane electrodialysis in saline wastewater treatment and the high-value conversion of salt resources into acids and alkalis.


Assuntos
Águas Residuárias , Purificação da Água , Álcalis , Membranas Artificiais , Purificação da Água/métodos , Membranas , Cloreto de Sódio
2.
Front Bioeng Biotechnol ; 10: 993436, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36246379

RESUMO

The frequency of chronic cutaneous wounds are sharply increasing in aging populations. Patients with age-related diseases, such as diabetes, tumors, renal failure and stroke are prone to soft tissue and skin injury, compounded by slowed healing in aging. Imbalance of wound inflammation, loss of growth factor secretion, and impairment of tissue repair abilities are all possible reasons for failed healing. Therefore, it is vital to explore novel approaches to accelerate wound healing. Platelet-rich plasma (PRP) as a cell therapy has been widely applied for tissue repair and regeneration. PRP promotes wound healing by releasing antimicrobial peptides, growth factors and micro-RNAs. Medical evidence indicates that autologous platelet-rich plasma (au-PRP) can promote wound healing effectively, safely and rapidly. However, its clinical application is usually restricted to patients with chronic cutaneous wounds, generally because of other severe complications and poor clinical comorbidities. Allogeneic platelet-rich plasma (al-PRP), with abundant sources, has demonstrated its superiority in the field of chronic wound treatment. Al-PRP could overcome the limitations of au-PRP and has promising prospects in clinical applications. The aim of this review is to summarize the current status and future challenges of al-PRP in chronic cutaneous wound management. We also summarized clinical cases to further describe the application of al-PRP for chronic wounds in clinical practice.

3.
Front Public Health ; 10: 1024864, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36276369

RESUMO

Objective: Obese or overweight is a risk factor for some chronic diseases, and oxidative stress and inflammation may be one of the molecular mechanisms leading to the persistence of these chronic diseases. Discovering interventions to alleviate oxidative stress and inflammation in the overweight/obese population, is very important for public health and health education. Methods: A two-week panel intervention study (Run 0-Run 1-Run 2) was conducted. The subjects were 77 overweight/obese undergraduates attending Dali University, with a BMI>24 kg/m2. The physical indices measured at the end of each run included BMI, waist circumference, serum ROS, TNF-α, IL-1ß and urinary 8-OHdG. Students were allocated to one of four intervention groups: No intervention (control); walking; taking vitamin C; and walking + taking vitamin C. Results: The results demonstrated (1) Walking significantly alleviated ROS levels, and this was consistent in Run 1 and Run 2; (2) During Run1, all three intervention modes reduced levels of 8-OHdG, but there was a statistically insignificant increase during Run 2; (3) No alleviating effects of the three intervention modes on TNF-α levels during Run 1 and Run 2 were observed; (4) The alleviating effects of the three intervention modes on IL-1ß levels during Run 1 and Run 2 were clear. Conclusion: Walking and taking vitamin C can reduce levels of ROS, 8-OHdG and IL-1ß, but not TNF-α, in overweight/obese participants. These interventions may become potential preventive measures for the overweight against obese-induced oxidative stress and inflammation.


Assuntos
Ácido Ascórbico , Sobrepeso , Humanos , Espécies Reativas de Oxigênio , Obesidade/epidemiologia , Inflamação , Estresse Oxidativo , Fator de Necrose Tumoral alfa , Caminhada , Estudantes
4.
Adv Mater ; 34(45): e2206269, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36106624

RESUMO

With the continuous breakthrough of the efficiency of organic photovoltaics (OPVs), their practical applications are on the agenda. However, the thickness tolerance and upscaling in recently reported high-efficiency devices remains challenging. In this work, the multiphase morphology and desired carrier behaviors are realized by utilizing a quaternary strategy. Notably, the exciton separation, carrier mobility, and carrier lifetime are enhanced significantly, the carrier recombination and the energy loss (Eloss ) are reduced, thus beneficial for a higher short-circuit density (JSC ), fill factor (FF), and open-circuit voltage (VOC ) of the quaternary system. Moreover, the intermixing-phase size is optimized, which is favorable for constructing the thick-film and large-area devices. Finally, the device with a 110 nm-thick active layer shows an outstanding power conversion efficiency (PCE) of 19.32% (certified 19.35%). Furthermore, the large-area (1.05 and 72.25 cm2 ) devices with 110 nm thickness present PCEs of 18.25% and 12.20%, and the device with a 305 nm-thick film (0.0473 cm2 ) delivers a PCE of 17.55%, which are among the highest values reported. The work demonstrates the potential of the quaternary strategy for large-area and thick-film OPVs and promotes the practical application of OPVs in the future.

5.
Environ Pollut ; 311: 119921, 2022 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-35973450

RESUMO

Distribution in ice is regarded as one of important transport modes for pollutants in seasonal freeze-up waters in cold regions. However, the distribution characteristics and mechanisms of fluorinated antibiotics as emerging contaminants during the water freezing process remain unclear. Here, florfenicol and norfloxacin were selected as model fluorinated antibiotics to investigate their ice-water distribution. Effects of antibiotic molecular structure on the distribution were explored through comparative studies with their non-fluorinated structural analogs. Results showed that phase changes during the ice growth process redistributed the antibiotics, with antibiotic concentrations in water 3.0-6.4 times higher than those in ice. The solute-rich boundary layer with a concentration gradient was presented at the ice-water interface and controlled by constitutional supercooling during the freezing process. The ice-water distribution coefficient (KIW) values of antibiotics increased by 34.8%-38.0% with a doubling of the cooling area. The solute distribution coefficient (Kbs) values of antibiotics at -20 °C were 65.6%-70.3% higher than at -10 °C. The KIW and Kbs values of all antibiotics were negatively correlated with their water solubilities. The fluorine substituents influenced the binding energies between antibiotics and ice, resulting in a 1.1-fold increase in the binding energy of norfloxacin on the ice surface relative to its structural analog pipemidic acid. The results provide a new insight into the transport behaviors of fluorinated pharmaceuticals in ice-water systems.


Assuntos
Gelo , Norfloxacino , Antibacterianos , Flúor , Congelamento , Tianfenicol/análogos & derivados
6.
Eur J Nutr ; 61(8): 4077-4089, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-35809101

RESUMO

PURPOSE: Chronic low-grade systemic inflammation affects muscle protein metabolism. The dietary inflammatory index (DII®) is a tool designed to assess the inflammatory potential of the diet. The available data on the association between DII and sarcopenia are limited. We aimed to investigate the association of the DII with components of sarcopenia in individuals over 50 years of age. METHODS: This cross-sectional study used the National Health and Nutrition Examination Survey (NHANES) 1999-2002 dataset. Body composition was measured, and isokinetic strength of the knee extensors (peak force) was evaluated. Low muscle mass and strength were defined using sex-specific thresholds. Energy-adjusted DII (E-DII™) scores were calculated using 24-h dietary recall data. Regression models were fit to evaluate the association between E-DII scores and low muscle mass and low muscle strength, alone and combined. RESULTS: Mean age of study participants was 62.1 ± 9.5 years, and 138 participants (7.4%) belonged to the combination group of low muscle mass and low muscle strength. In multivariable-adjusted regression models, higher E-DII score was associated with lower appendicular skeletal muscle index (ASMI) (ß = - 0.03, P  < 0.001, P trend <0.001), and lower peak force (ß = -2.15, P  = 0.04, P trend = 0.01) and higher likelihood for these components combined (OR = 1.12, 95% CI 1.01-1.25, P = 0.03). CONCLUSION: Higher E-DII score is associated with lower muscle mass and muscle strength, and increased likelihood for the combination of low muscle mass and low muscle strength in older adults. This has important implications for healthy aging.


Assuntos
Sarcopenia , Masculino , Feminino , Humanos , Pessoa de Meia-Idade , Idoso , Inquéritos Nutricionais , Estudos Transversais , Sarcopenia/epidemiologia , Dieta , Inflamação/metabolismo , Músculos/metabolismo , Proteínas Musculares
7.
Sci Rep ; 12(1): 11349, 2022 07 05.
Artigo em Inglês | MEDLINE | ID: mdl-35790766

RESUMO

Following significant advances in image acquisition, synapse detection, and neuronal segmentation in connectomics, researchers have extracted an increasingly diverse set of wiring diagrams from brain tissue. Neuroscientists frequently represent these wiring diagrams as graphs with nodes corresponding to a single neuron and edges indicating synaptic connectivity. The edges can contain "colors" or "labels", indicating excitatory versus inhibitory connections, among other things. By representing the wiring diagram as a graph, we can begin to identify motifs, the frequently occurring subgraphs that correspond to specific biological functions. Most analyses on these wiring diagrams have focused on hypothesized motifs-those we expect to find. However, one of the goals of connectomics is to identify biologically-significant motifs that we did not previously hypothesize. To identify these structures, we need large-scale subgraph enumeration to find the frequencies of all unique motifs. Exact subgraph enumeration is a computationally expensive task, particularly in the edge-dense wiring diagrams. Furthermore, most existing methods do not differentiate between types of edges which can significantly affect the function of a motif. We propose a parallel, general-purpose subgraph enumeration strategy to count motifs in the connectome. Next, we introduce a divide-and-conquer community-based subgraph enumeration strategy that allows for enumeration per brain region. Lastly, we allow for differentiation of edges by types to better reflect the underlying biological properties of the graph. We demonstrate our results on eleven connectomes and publish for future analyses extensive overviews for the 26 trillion subgraphs enumerated that required approximately 9.25 years of computation time.


Assuntos
Conectoma , Encéfalo/diagnóstico por imagem , Neurônios , Editoração , Sinapses
8.
Sci Rep ; 12(1): 11714, 2022 07 09.
Artigo em Inglês | MEDLINE | ID: mdl-35810186

RESUMO

SARS-CoV-2 infection triggers profound and variable immune responses in human hosts. Chromatin remodeling has been observed in individuals severely ill or convalescing with COVID-19, but chromatin remodeling early in disease prior to anti-spike protein IgG seroconversion has not been defined. We performed the Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq) and RNA-seq on peripheral blood mononuclear cells (PBMCs) from outpatients with mild or moderate symptom severity at different stages of clinical illness. Early in the disease course prior to IgG seroconversion, modifications in chromatin accessibility associated with mild or moderate symptoms were already robust and included severity-associated changes in accessibility of genes in interleukin signaling, regulation of cell differentiation and cell morphology. Furthermore, single-cell analyses revealed evolution of the chromatin accessibility landscape and transcription factor motif accessibility for individual PBMC cell types over time. The most extensive remodeling occurred in CD14+ monocytes, where sub-populations with distinct chromatin accessibility profiles were observed prior to seroconversion. Mild symptom severity was marked by upregulation of classical antiviral pathways, including those regulating IRF1 and IRF7, whereas in moderate disease, these classical antiviral signals diminished, suggesting dysregulated and less effective responses. Together, these observations offer novel insight into the epigenome of early mild SARS-CoV-2 infection and suggest that detection of chromatin remodeling in early disease may offer promise for a new class of diagnostic tools for COVID-19.


Assuntos
COVID-19 , Cromatina , Antivirais , COVID-19/genética , Cromatina/genética , Humanos , Imunoglobulina G/genética , Leucócitos Mononucleares , SARS-CoV-2 , Soroconversão , Índice de Gravidade de Doença
9.
Environ Int ; 165: 107315, 2022 07.
Artigo em Inglês | MEDLINE | ID: mdl-35635966

RESUMO

BACKGROUND: Increasing prevalence of childhood allergic rhinitis(AR) needs a deeper understanding on the potential adverse effects of early life exposure to air pollution. OBJECTIVES: The main aim was to evaluate the effects of maternal exposure to PM2.5 and chemical constituents during pregnancy on preschool children's AR, and further to explore the modification effects of regions and exclusive breastfeeding. METHODS: A multi-center population-based study was performed in 6 cities from 3 regions of China in 2011-2012. Maternal exposure to ambient PM2.5 and main chemical constituents(BC, OM, SO42-, NO3-, NH4+) during pregnancy was assessed and a longitudinal prospective analysis was applied on preschool children's AR. The modification effects of regions and exclusive breastfeeding were investigated. RESULTS: A total of 8.8% and 9.8% of children reported doctor-diagnosed allergic rhinitis(DDAR) and current hay fever, respectively, and 48.6% had less than 6 months of exclusive breastfeeding. The means of PM2.5 during pregnancy were 52.7 µg/m3, 70.3 µg/m3 and 76.4 µg/m3 in the east, north and central south of China, respectively. Multilevel log-binomial model regression showed that each interquartile range(IQR) increase of PM2.5 during pregnancy was associated with an average increase in prevalence ratio (PR) of DDAR by 1.43(95% confidence interval(CI): 1.11, 1.84) and current hay fever by 1.79(95% CI: 1.26, 2.55), respectively. Among chemical constituents, black carbon (BC) had the strongest associations. Across 3 regions, the eastern cities had the highest associations, followed by those in the central south and the north. For those equal to or longer than 6 months of exclusive breastfeeding, the associations were significantly reduced. CONCLUSIONS: Children in east of China had the highest risks of developing AR per unit increase of maternal exposure to PM2.5 during pregnancy, especially BC constituent. Remarkable decline was found in association with an increase in breastfeeding for ≥6 months, in particular in east of China.


Assuntos
Poluentes Atmosféricos , Rinite Alérgica Sazonal , Rinite Alérgica , Poluentes Atmosféricos/análise , Aleitamento Materno , Pré-Escolar , Feminino , Humanos , Exposição Materna/efeitos adversos , Material Particulado/análise , Gravidez , Rinite Alérgica/epidemiologia , Rinite Alérgica/etiologia , Rinite Alérgica Sazonal/induzido quimicamente , Fuligem/análise
10.
Res Sq ; 2022 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-35411343

RESUMO

SARS-CoV-2 infection triggers profound and variable immune responses in human hosts. Chromatin remodeling has been observed in individuals severely ill or convalescing with COVID-19, but chromatin remodeling early in disease prior to anti-spike protein IgG seroconversion has not been defined. We performed the Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq) and RNA-seq on peripheral blood mononuclear cells (PBMCs) from outpatients with mild or moderate symptom severity at different stages of clinical illness. Early in the disease course prior to IgG seroconversion, modifications in chromatin accessibility associate with mild or moderate symptoms are already robust and include severity-associated changes in accessibility of genes in interleukin signaling, regulation of cell differentiation and cell morphology. Furthermore, single-cell analyses revealed evolution of the chromatin accessibility landscape and transcription factor motif accessibility for individual PBMC cell types over time. The most extensive remodeling occurred in CD14+ monocytes, where sub-populations with distinct chromatin accessibility profiles were observed prior to seroconversion. Mild symptom severity is marked by upregulation classical antiviral pathways including those regulating IRF1 and IRF7, whereas in moderate disease these classical antiviral signals diminish suggesting dysregulated and less effective responses. Together, these observations offer novel insight into the epigenome of early mild SARS-CoV-2 infection and suggest that detection of chromatin remodeling in early disease may offer promise for a new class of diagnostic tools for COVID-19.

11.
Chem Asian J ; 17(11): e202200127, 2022 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-35344261

RESUMO

The oxygen evolution reaction is of great significance to the production of hydrogen from high efficiency electrolytic water, hydrogen oxygen fuel cell and other energy conversion devices, but there are many challenges such as high cost, low efficiency and poor stability of catalysts. Among non-precious metal catalysts, oxide has its unique advantages. We used overdoping strategy to prepare two-phase oxide electrocatalyst SrCo0.9 Fe0.05 Mo0.35 Ox (SCFM0.35 ) containing double perovskite and Co3 O4 with excellent OER electrocatalytic activity and stability in alkaline solution. It required an overpotential of 361.7 mV to reach a 10 mA cm-2 current density and its performance only degrades by 3.48% after 1000 CV cycles accelerated stability tests, whose electrochemical performance is superior to that of single-phase double perovskites and undoped perovskites. SrCo0.9 Fe0.1 O3 (SCF) ordinary perovskite is doped with slightly molybdenum (Mo), and then the ordinary perovskite turns into double perovskite because of the polyvalence characteristics of Mo. When Mo is overdoped, Co3 O4 phase was precipitated while Mo entered perovskite phase. This process causes lattice distortion and makes the surface electronic structure benign changes. Furthermore, the microscopic morphology of the material surface and the valence state of cobalt element are changed, thereby improving the microenvironment of the electrochemical process.

12.
Adv Mater ; 34(17): e2200044, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35236010

RESUMO

Developing indium-tin-oxide (ITO)-free flexible organic photovoltaics (OPVs) with upscaling capacity is of great significance for practical applications of OPVs. Unfortunately, the efficiencies of the corresponding devices lag far behind those of ITO-based rigid small-area counterparts. To address this issue, an advanced device configuration is designed and fabricated featuring a top-illuminated structure with ultrathin Ag as the transparent electrode. First, a conjugated polyelectrolyte layer, i.e., PCP-Li, is inserted to effectively connect the bottom Ag anode and the hole transport layer, achieving good photon to electron conversion. Second, charge collecting grids are deposited to suppress the increased resistance loss with the upscaling of the device area, realizing almost full retention of device efficiency from 0.06 to 1 cm2 . Third, the designed device delivers the best efficiency of 15.56% with the area of 1 cm2 on polyimide substrate, representing as the record among the ITO-free, large-area, flexible OPVs. Interestingly, the device exhibits no degradation after 100 000 bending cycles with a radius of 4 mm, which is the best result for flexible OPVs. This work provides insight into device structure design and optimization for OPVs with high efficiency, low cost, superior flexibility, and upscaling capacity, indicating the potential for the future commercialization of OPVs.

13.
Appl Environ Microbiol ; 88(7): e0221521, 2022 04 12.
Artigo em Inglês | MEDLINE | ID: mdl-35311507

RESUMO

Rhodotorula mucilaginosa shows adaption to a broad range of Pb2+ stress. In this study, three key pathways, i.e., glycolysis (EMP), the tricarboxylic acid (TCA) cycle, and oxidative phosphorylation (OXPHOS), were investigated under 0-2,500 mg · L-1 Pb stress, primarily based on biochemical analysis and RNA sequencing. R. mucilaginosa cells showed similar metabolic response to low/medium (500/1000 mg · L-1) Pb2+ stress. High (2,500 mg · L-1) Pb2+ stress exerted severe cytotoxicity to R. mucilaginosa. The downregulation of HK under low-medium Pb2+ suggested a correlation with the low hexokinase enzymatic activity in vivo. However, IDH3, regulating a key step of circulation in TCA, was upregulated to promote ATP feedstock for downstream OXPHOS. Then, through activation of complex I & IV in the electron transport chain (ETC) and ATP synthase, ATP production was finally enhanced. This mechanism enabled fungal cells to compensate for ATP consumption under low-medium Pb2+ toxicity. Hence, R. mucilaginosa tolerance to such a broad range of Pb2+ concentrations can be attributed to energy adaption. In contrast, high Pb2+ stress caused ATP deficiency. Then, the subsequent degradation of intracellular defense systems further intensified Pb toxicity. This study correlated responses of EMP, TCA, and OXPHOS pathways in R. mucilaginosa under Pb stress, hence providing new insights into the fungal resistance to heavy metal stress. IMPORTANCE Glycolysis (EMP), the tricarboxylic acid (TCA) cycle, and oxidative phosphorylation (OXPHOS) are critical metabolism pathways for microorganisms to obtain energy during the resistance to heavy metal (HM) stress. However, these pathways at the genetic level have not been elucidated to evaluate their cytoprotective functions for Rhodotorula mucilaginosa under Pb stress. In this study, we investigated these three pathways based on biochemical analysis and RNA sequencing. Under low-medium (500-1,000 mg · L-1) Pb2+ stress, ATP production was stimulated mainly due to the upregulation of genes associated with the TCA cycle and the electron transport chain (ETC). Such an energy compensatory mechanism could allow R. mucilaginosa acclimation to a broad range of Pb2+ concentrations (up to 1000 mg · L-1). In contrast, high (2500 mg · L-1) Pb2+ stress exerted its excessive toxicity by provoking ATP deficiency and damage to intracellular resistance systems. This study provided new insights into R. mucilaginosa resistance to HM stress from the perspective of metabolism.


Assuntos
Chumbo , Metais Pesados , Trifosfato de Adenosina , Ciclo do Ácido Cítrico , Perfilação da Expressão Gênica , Chumbo/toxicidade , Rhodotorula , Ácidos Tricarboxílicos
14.
NPJ Digit Med ; 5(1): 18, 2022 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-35165389

RESUMO

The COVID-19 pandemic has pushed healthcare systems globally to a breaking point. The urgent need for effective and affordable COVID-19 treatments calls for repurposing combinations of approved drugs. The challenge is to identify which combinations are likely to be most effective and at what stages of the disease. Here, we present the first disease-stage executable signalling network model of SARS-CoV-2-host interactions used to predict effective repurposed drug combinations for treating early- and late stage severe disease. Using our executable model, we performed in silico screening of 9870 pairs of 140 potential targets and have identified nine new drug combinations. Camostat and Apilimod were predicted to be the most promising combination in effectively supressing viral replication in the early stages of severe disease and were validated experimentally in human Caco-2 cells. Our study further demonstrates the power of executable mechanistic modelling to enable rapid pre-clinical evaluation of combination therapies tailored to disease progression. It also presents a novel resource and expandable model system that can respond to further needs in the pandemic.

15.
Aging Cell ; 21(2): e13530, 2022 02.
Artigo em Inglês | MEDLINE | ID: mdl-34984806

RESUMO

Older age is a major risk factor for damage to many tissues, including liver. Aging undermines resiliency and impairs liver regeneration. The mechanisms whereby aging reduces resiliency are poorly understood. Hedgehog is a signaling pathway with critical mitogenic and morphogenic functions during development. Recent studies indicate that Hedgehog regulates metabolic homeostasis in adult liver. The present study evaluates the hypothesis that Hedgehog signaling becomes dysregulated in hepatocytes during aging, resulting in decreased resiliency and therefore, impaired regeneration and enhanced vulnerability to damage. Partial hepatectomy (PH) was performed on young and old wild-type mice and Smoothened (Smo)-floxed mice treated with viral vectors to conditionally delete Smo and disrupt Hedgehog signaling specifically in hepatocytes. Changes in signaling were correlated with changes in regenerative responses and compared among groups. Old livers had fewer hepatocytes proliferating after PH. RNA sequencing identified Hedgehog as a top downregulated pathway in old hepatocytes before and after the regenerative challenge. Deleting Smo in young hepatocytes before PH prevented Hedgehog pathway activation after PH and inhibited regeneration. Gene Ontogeny analysis demonstrated that both old and Smo-deleted young hepatocytes had activation of pathways involved in innate immune responses and suppression of several signaling pathways that control liver growth and metabolism. Hedgehog inhibition promoted telomere shortening and mitochondrial dysfunction in hepatocytes, consequences of aging that promote inflammation and impair tissue growth and metabolic homeostasis. Hedgehog signaling is dysregulated in old hepatocytes. This accelerates aging, resulting in decreased resiliency and therefore, impaired liver regeneration and enhanced vulnerability to damage.


Assuntos
Proteínas Hedgehog , Transdução de Sinais , Envelhecimento , Animais , Proliferação de Células , Proteínas Hedgehog/metabolismo , Hepatócitos/metabolismo , Fígado/metabolismo , Regeneração Hepática/fisiologia , Camundongos
16.
Sci Total Environ ; 820: 153272, 2022 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-35074375

RESUMO

Carbon dioxide (CO2) capture coupled with further mineralization in high value-added form is a great challenge for carbon capture utilization and storage (CCUS) processes. In this work, a bipolar membrane electrodialysis (BMED) technique integrated with crystallization chamber was proposed to utilize CO2-derived carbonates and the residual magnesium resource from seawater to produce functional nesquehonite. To ensure the stable CO2 storage and magnesium extraction by BMED process, the metastable zone during nesquehonite crystallizing was first measured to modulate crystallization rate, obtain high-quality crystal products and inhibit membrane fouling states. Subsequently, the effects of current density, temperature, and CO2 flow rate during the whole BMED-crystallization process were further investigated. The increase in current density and temperature was conducive for the extraction of magnesium while the enlarged gas flow rate induced higher absorption of CO2. Under the current density at 22 A/m2, CO2 flow rate at 50 mL/min and temperature at 30 °C, the optimal carbon absorption ratio and the magnesium extraction ratio reached 50.85% and 56.71%, respectively. Under this condition, the explosion nucleation of the nesquehonite was effectively avoided to inhibit membrane fouling and the generation of magnesium hydroxide was depressed to obtain the target product nesquehonite. This study on simultaneous carbon capture and magnesium utilization provides theoretical guidance for the industrial green storage of CO2 and development of valuable magnesium products.


Assuntos
Dióxido de Carbono , Magnésio , Dióxido de Carbono/química , Carbonatos , Magnésio/química , Água do Mar/química , Temperatura
17.
Arch Osteoporos ; 17(1): 24, 2022 01 26.
Artigo em Inglês | MEDLINE | ID: mdl-35080671

RESUMO

INTRODUCTION: This cross-sectional study aimed to investigate the relationship between abdominal aortic calcification (AAC), which is a marker of vascular calcification, and volumetric bone mineral density (vBMD) by quantitative computed tomography (QCT) in maintenance hemodialysis (MHD) patients. METHODS: All participants underwent lumbar vertebral vBMD measurement by QCT. Eight cross-sections were extracted sequentially and analyzed by ImageJ software to obtain the ratio of the calcified area to the abdominal aortic area (the calcification ratio). The AAC score was determined by the sum of the calcification ratios. The relationship between AAC and vBMD was analyzed using multivariate logistic regression. RESULTS: Ninety MHD patients (58.89% male) with a mean age of 63.43 (standard deviation [SD] = 13.20) years were included in the study. AAC was present (AAC score > 0) in 93.33% of the patients. The 75th percentile of the AAC score corresponding to 119 was used as the cutoff point between the mild and severe groups. After full adjustment in the logistic model, AAC was found to be inversely associated with vBMD (odds ratio [OR], 0.970; 95% confidence interval [CI], 0.944 to 0.996; P = 0.025), and patients with osteoporosis had a significantly higher risk of severe AAC than those with normal bone mass (OR, 14.498; 95% CI, 1.507 to 139.486; P = 0.021). The independent inverse association was still stable after adjusting for variables measured at different time periods and using different cutoff points of the AAC score. CONCLUSION: There was an independent inverse association between AAC and vBMD, and osteoporosis was significantly associated with severe AAC in patients with MHD.


Assuntos
Densidade Óssea , Calcificação Vascular , Aorta Abdominal/diagnóstico por imagem , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Diálise Renal , Tomografia Computadorizada por Raios X/métodos , Calcificação Vascular/diagnóstico por imagem , Calcificação Vascular/epidemiologia
19.
IEEE Trans Pattern Anal Mach Intell ; 44(4): 2031-2044, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33095709

RESUMO

This paper focuses on communication-efficient federated learning problem, and develops a novel distributed quantized gradient approach, which is characterized by adaptive communications of the quantized gradients. Specifically, the federated learning builds upon the server-worker infrastructure, where the workers calculate local gradients and upload them to the server; then the server obtain the global gradient by aggregating all the local gradients and utilizes it to update the model parameter. The key idea to save communications from the worker to the server is to quantize gradients as well as skip less informative quantized gradient communications by reusing previous gradients. Quantizing and skipping result in 'lazy' worker-server communications, which justifies the term Lazily Aggregated Quantized (LAQ) gradient. Theoretically, the LAQ algorithm achieves the same linear convergence as the gradient descent in the strongly convex case, while effecting major savings in the communication in terms of transmitted bits and communication rounds. Empirically, extensive experiments using realistic data corroborate a significant communication reduction compared with state-of-the-art gradient- and stochastic gradient-based algorithms.

20.
J Appl Toxicol ; 42(6): 1067-1077, 2022 06.
Artigo em Inglês | MEDLINE | ID: mdl-34967033

RESUMO

Anisodamine is one of the major components of the tropine alkaloid family and is widely used in the treatment of pain, motion sickness, pupil dilatation, and detoxification of organophosphorus poisoning. As a muscarinic receptor antagonist, the low toxicity and moderate drug effect of anisodamine often result in high doses for clinical use, making it important to fully investigate its toxicity. In this study, zebrafish embryos were exposed to 1.3-, 2.6-, and 5.2-mM anisodamine for 7 days to study the toxic effects of drug exposure on pigmentation, mineral density, craniofacial area, and eye development. The results showed that exposure to anisodamine at 1.3 mM resulted in cranial malformations and abnormal pigmentation in zebrafish embryos; 2.6- and 5.2-mM anisodamine resulted in significant eye development defects and reduced bone density in zebrafish embryos. The associated toxicities were correlated with functional development of neural crest cells through gene expression (col1a2, ddb1, dicer1, mab21l1, mab21l2, sox10, tyrp1b, and mitfa) in the dose of 5.2-mM exposed group. In conclusion, this study provides new evidence of the developmental toxicity of high doses of anisodamine in aqueous solutions to organisms and provides a warning for the safe use of this drug.


Assuntos
Alcaloides de Solanáceas , Peixe-Zebra , Animais , Embrião não Mamífero , Minerais/metabolismo , Minerais/farmacologia , Pigmentação , Alcaloides de Solanáceas/metabolismo , Alcaloides de Solanáceas/farmacologia , Alcaloides de Solanáceas/uso terapêutico , Peixe-Zebra/genética , Proteínas de Peixe-Zebra/genética
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