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1.
Neural Regen Res ; 16(1): 80-92, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32788451

RESUMO

Stroke is a main cause of death and disability worldwide. The ability of the brain to self-repair in the acute and chronic phases after stroke is minimal; however, promising stem cell-based interventions are emerging that may give substantial and possibly complete recovery of brain function after stroke. Many animal models and clinical trials have demonstrated that neural stem cells (NSCs) in the central nervous system can orchestrate neurological repair through nerve regeneration, neuron polarization, axon pruning, neurite outgrowth, repair of myelin, and remodeling of the microenvironment and brain networks. Compared with other types of stem cells, NSCs have unique advantages in cell replacement, paracrine action, inflammatory regulation and neuroprotection. Our review summarizes NSC origins, characteristics, therapeutic mechanisms and repair processes, then highlights current research findings and clinical evidence for NSC therapy. These results may be helpful to inform the direction of future stroke research and to guide clinical decision-making.

2.
Food Chem ; 337: 127783, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-32791427

RESUMO

Jujube slices were pretreated by cold plasma for 15, 30, and 60 s on each side, followed by hot air drying at 50, 60, and 70 °C. Scanning electron microscopy investigation indicated that the application of cold plasma significantly changed the surface topography of jujube slice by etching larger cavities, which can facilitate moisture transfer and consequently enhance drying rate and effective diffusivity. Modified Henderson & Pabis model and Two-term model were the two most recommended models for describing the drying kinetics of jujube slices. Cold plasma pretreatment improved the contents of procyanidins, flavonoids, and phenolics by 53.81%, 33.89%, and 13.85% at most, respectively, and thereby enhanced antioxidant capacity by 36.85% at most. Besides, cold plasma pretreatment can reduce the production of 5-hydroxymethylfurfural by 52.19% at most. In summary, cold plasma can be used as a promising pretreatment tool for drying processes of jujube slices.

3.
Food Chem ; 337: 127654, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-32791428

RESUMO

This study aimed to improve the physicochemical stability of nanoliposome (NL) with enhanced functionality for the delivery of Pelargonidin-3-O-glucoside (P3G) using biopolymers, i.e. chitosan (CH) and pectin (P). In this study, we successfully developed stabilized liposomal carriers, i.e. CH-conjugated NL (CH-NL) and P-conjugated CH-NL (P-CH-NL) using an optimum concentration of CH (0.6 wt%) and P (0.5 wt%). Results revealed that P-CH-NL had better physical stability to salt and pH with maximum P3G retention (>97%) under oxidative, thermal, and UV conditions. Nanoliposomes were more stable under refrigerated-storage and ensured high P3G retention (>96%). In vitro mucoadhesion study revealed that CH-NL had better mucin adsorption efficiency (59.72%) followed by P-CH-NL and NL. Furthermore, CH-NL and P-CH-NL alternatively had better stability to serum than NL. Taken together, the stabilization of nanoliposome using chitosan and pectin can be a promising approach for the delivery of hydrophilic compounds in association with enhanced stability and functionality.

4.
Viruses ; 12(10)2020 09 30.
Artigo em Inglês | MEDLINE | ID: mdl-33007975

RESUMO

Grapevine leafroll-associated virus 1 (GLRaV-1) is a major pathogen associated with grapevine leafroll disease. However, the molecular mechanisms underlying GLRaV-1 interactions with plant cells are unclear. Using Agrobacterium infiltration-mediated RNA-silencing assays, we demonstrated that GLRaV-1 p24 protein (p24G1) acts as an RNA-silencing suppressor (RSS), inhibiting local and systemic RNA silencing. Electrophoretic mobility shift assays showed that p24G1 binds double-stranded 21-nucleotide small interfering RNA (siRNA), and that siRNA binding is required but not sufficient for its RSS activity. p24G1 localizes in the nucleus and can self-interact through its amino acid 10 to 210 region. Dimerization is needed for p24G1 interaction with importin α1 before moving to the nucleus, but is not required for its siRNA binding and RSS activity. Expression of p24G1 from a binary pGD vector or potato virus X-based vector elicited a strong hypersensitive response in Nicotiana species, indicating that p24G1 may be a factor in pathogenesis. Furthermore, p24G1 function in pathogenesis required its RSS activity, dimerization and nuclear localization. In addition, the region of amino acids 122-139 played a crucial role in the nuclear import, siRNA binding, silencing suppression and pathogenic activity of p24G1. These results contribute to our understanding of the molecular mechanisms underlying GLRaV-1 infection.

5.
Clin Nutr ; 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-33039156

RESUMO

BACKGROUND & AIMS: Micturition dysfunction is a problem in the general population that progresses with aging in both males and females. In the past few decades, the relationship between voiding symptoms and body biochemical status has been a subject of research in several disciplines. Micronutrition is considered to affect different aspects of urinary flow, including neuroregulation, detrusor muscle function, and the structures around the bladder outlet such as the pelvic floor and prostate. Therefore, the objective of our study was to determine the correlation between urine flow rate (UFR) and 25-hydroxyvitamin D (25(OH)D) in the general healthy population. METHODS: Our study involved 3981 adult participants over age 20 from the U.S. National Health and Nutrition Examination Survey datasets (2011-2012). The associations between UFR and serum 25(OH)D concentration were analyzed through multivariate regression models. RESULTS: There was a significant positive association of serum 25(OH)D concentration with UFR (25(OH)D2+25(OH)D3: ß coefficient: 0.003; 95% CI: 0.002, 0.004; p < 0.001, 25(OH)D3; p = 0.003; epi-25(OH)D3, p = 0.020) in an unadjusted model. The substantial associations were still observed in the gender and age subgroups. In analysis of age subgroup, the association of serum 25(OH)D concentration with urine flow rate was significant in fully adjusted model (age<60: 25(OH)D2+25(OH)D3: ß coefficient: 0.004, p < 0.001; 25(OH)D3: p = <0.001, epi-25(OH)D3: p = 0.007; Age≥60: 25(OH)D2+25(OH)D3: ß coefficient: 0.004, p = 0.002; 25(OH)D3: p = 0.001, epi-25(OH)D3: p = 0.001). In gender subgroup analysis, the ß coefficient of 25(OH)D2+25(OH)D3 in male is 0.004 (p < 0.001), and in female is 0.004 (p < 0.001) in fully adjusted model. The higher quartiles of UFR tended to have higher 25(OH)D3 levels with statistically significant in quartile-based analysis. CONCLUSIONS: UFR was associated with increased level of total vitamin D and bioactive form vitamin D3. Vitamin D supplements may be a simple and effective way of improving of bladder function.

7.
J Biotechnol ; 2020 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-33039549

RESUMO

Lipid hyperaccumulation in oleaginous microorganisms is generally induced by nitrogen limitation, while oxygen supply can influence biomass growth and cell metabolism. Although strategies based on nitrogen limitation or oxygen control have been extensively explored and applied in various oleaginous microorganisms, the role of oxygen supply in nitrogen limitation induced lipid hyperaccumulation still remains unclear. Here, we systematically surveyed the effects of oxygen supply on the oleaginous fungus M. alpina cultured in nitrogen limited conditions through integration of physiochemical parameters and metabolomics analysis. Our results indicated that a high oxygen supply promoted carbon/nitrogen consumption and was used for rapid biomass synthesis, while either high or low oxygen supply conditions were adverse to lipid and ARA accumulation. Different oxygen supply level significantly affected the balance between fermentation for lipid synthesis and respiration for energy generation. Under nitrogen limitation, a suitable oxygen supply promoted the recycling of preformed nitrogen and increased the redirection of carbon towards fatty acid synthesis through the hub centred around glutamic acid coupled to the intermediate metabolism of carbon in the TCA cycle, while a high oxygen supply favored the respiration process and enhanced the degradation of LC-PUFAs, rather than fermentation for fatty acid synthesis. This system-level insight reveals the underlying metabolic mechanism of oxygen control in nitrogen limitation induced lipid accumulation, and provides theoretical support for the integration of oxygen control with nutrient supply for efficient microbial oil production.

8.
Artigo em Inglês | MEDLINE | ID: mdl-33039642

RESUMO

BACKGROUND: Bullous skin disorders are induced by different pathomechanisms and several are emergent, including Stevens-Johnson syndrome/toxic epidermal necrolysis (SJS/TEN). Rapid diagnostic methods for SJS/TEN or cytotoxic T lymphocyte (CTL)-mediated bullous disorders are crucial for early treatment. Granulysin, primarily expressed by CTLs, is a specific cytotoxic protein responsible for SJS/TEN and similar skin reactions.background OBJECTIVE: To assess granulysin levels in blister fluids to differentiate SJS/TEN and similar CTL-mediated bullous reactions from other autoimmune bullous disorders. METHODS: Using enzyme-linked immunosorbent assay, we measured granulysin in blister fluids from patients with bullous skin disorders, including SJS/TEN, erythema multiforme major (EMM), bullous fixed-drug eruption (BFDE), bullous lupus erythematosus (LE), paraneoplastic pemphigus (PNP), pemphigus vulgaris (PV), bullous pemphigoid (BP), purpura fulminans-related bullae, and hand-foot syndrome (HFS)/hand-foot-skin reactions (HFSR). We compared serum and blister granulysin levels in SJS/TEN patients presenting varying severity, monitoring serial granulysin levels from acute to late stages. RESULTS: Overall, 146 patients presenting bullous skin disorders were enrolled. Blister granulysin levels (mean±SD) in CTL-mediated disorders, including TEN (n=28, 3938.7±3475.7), SJS-TEN overlapping (n=22, 1440.4±1179.6), SJS (n=14, 542.0±503.2), EMM (n=7, 766.3±1073.7), generalized BFDE (n=10, 720.4±858.3), and localized BFDE (n=16, 69.0±56.4) were significantly higher than in non-CTL-mediated bullous disorders (p<0.0001), including bullous LE (n=3, 22.7±20.1), PNP (n=3, 20.3±8.6), PV (n=3, 4.4±2.8), BP (n=18, 4.04±2.65), purpura fulminans (n=4, 5.88±5.46), and HFS/HFSR(n=5, 4.64±3.46). Blister granulysin levels correlated with clinical severity of SJS/TEN (p<0.0001).results CONCLUSION: Determination of blister granulysin levels is a noninvasive and useful tool for rapid differential diagnosis of SJS/TEN and other similar CTL-mediated bullous skin disorders for treatment selection.

9.
BMJ Open ; 10(10): e037842, 2020 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-33040002

RESUMO

INTRODUCTION: The prevalence of hyperuricaemia in peritoneal dialysis patients is quite high. Studies have demonstrated a correlation between hyperuricaemia and cardiovascular disease and treatment of hyperuricaemia reportedly reduces cardiovascular risk in patients with chronic kidney disease. However, whether hyperuricaemia treatment benefits cardiovascular outcomes in continuous ambulatory peritoneal dialysis (CAPD) patients is not yet known. METHODS AND ANALYSES: This prospective, multicentre, double-blind, randomised controlled trial was designed to evaluate the effects of hyperuricaemia treatment on cardiovascular event risk in CAPD patients. Based on a power of 80%, with type I error α=0.05, two-sided test and 1:1 parallel control study, considering a dropout rate of 20%, a total of 548 eligible patients are expected to be randomly assigned to either the hyperuricaemia treatment group (febuxostat) or control group (placebo). ETHICS AND DISSEMINATION: This study has been approved by the Medical Ethics Committee of the First Affiliated Hospital, Sun Yat-sen University and the ethics committees of other participating institutions. Written informed consent will be obtained from potential trial participants or authorised surrogates.The findings of the study will be disseminated through publications in peer-reviewed journals, and presentations at national and international conferences. TRIAL REGISTRATION NUMBER: NCT03200210. 25 June 2017. The trial was started on 13 July 2017, and is expected to end by 31 December 2022. Till 20 Jan 2020, a total of 548 patients have been recruited. PROTOCOL VERSION: The protocol version number and date are YLT-1604-V2.0 and 15 December 2016.

10.
Surg Radiol Anat ; 2020 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-33040160

RESUMO

PURPOSE: The aim of the present study was to identify the hepatic vascular variations with visual three-dimensional (3D) reconstruction of vessels in pediatric patients with choledochal cyst (CDC). METHODS: We retrospectively analyzed the data of 84 children with pathologically confirmed CDCs treated in the Children's Hospital of Soochow University. 180 patients without CDCs as a control to analysis the hepatic artery and portal vein anatomy. All patients were examined by multi-slice spiral CT (MSCT) and the images of children with CDC were reconstructed by Hisense computer-assisted surgery system (Hisense CAS) to obtain visual 3D images. RESULTS: There were 71 females and 13 males diagnosed with CDC. According to Todani classification of CDC, there were 42 cases of type Ia, 10 cases of type Ic and 32 cases of type IVa. There were 10 (11.9%) patients with hepatic artery variations, 14 (16.7%) patients with right hepatic artery located on the ventral side of the CDC, and 16 (19.0%) patients with portal vein variations. Sex, age and types of the cyst were not associated with the presence of vascular variations. There was no significant difference in hepatic vascular variation between CDCs and control groups. Visual 3D images clearly displayed the hepatic vascular variations and the spatial structure of the CDC in pediatric patients with CDC. CONCLUSIONS: Hepatic artery and portal vein variations can be detected in pediatric patients with CDC. Visual 3D technique can visually and stereoscopically display the anatomical variations of the hepatic artery and portal vein.

11.
J Neural Eng ; 17(5): 056011, 2020 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-33043903

RESUMO

OBJECTIVE: Removal of common mode noise and artifacts from extracellularly measured action potentials, herein referred to as spikes, recorded with multi-electrode arrays (MEAs) which included severe noise and artifacts generated by an ultrahigh field (UHF) 16.4 Tesla magnetic resonance imaging (MRI) scanner. APPROACH: An adaptive virtual referencing (AVR) algorithm is used to remove artifacts and thus enable extraction of neural spike signals from extracellular recordings in anesthetized rat brains. A 16-channel MEA with 150-micron inter-site spacing is used, and a virtual reference is created by spatially averaging the 16 signal channels which results in a reference signal without extracellular spiking activity while preserving common mode noise and artifacts. This virtual reference signal is then used as the input to an adaptive FIR filter which optimally scales and time-shifts the reference to each specific electrode site to remove the artifacts and noise. MAIN RESULTS: By removing artifacts and reducing noise, the neural spikes at each electrode site can be well extracted, even from data originally recorded with a high noise floor due to electromagnetic interference and artifacts generated by a 16.4T MRI scanner. The AVR method enables many more spikes to be detected than would otherwise be possible. Further, the filtered spike waveforms can be well separated from each other using PCA feature extraction and semi-supervised k-means clustering. While data in a 16.4T MRI scanner contains significantly more noise and artifacts, the developed AVR method enables similar data quality to be extracted as recorded on benchtop experiments outside the MRI scanner. SIGNIFICANCE: AVR of extracellular spike signals recorded with MEAs has not been previously reported and fills a technical need by enabling low-noise extracellular spike extraction in noisy and challenging environments such as UHF MRI that will enable further study of neuro-vascular coupling at UHF.

12.
Prog Brain Res ; 255: 207-247, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33008507

RESUMO

The visual network is crucially implicated in the pathophysiology of migraine. Several lines of evidence indicate that migraine is characterized by an altered visual cortex excitability both during and between attacks. Visual symptoms, the most common clinical manifestation of migraine aura, are likely the result of cortical spreading depression originating from the extrastriate area V3A. Photophobia, a clinical hallmark of migraine, is linked to an abnormal sensory processing of the thalamus which is converged with the non-image forming visual pathway. Finally, visual snow is an increasingly recognized persistent visual phenomenon in migraine, possibly caused by increased perception of subthreshold visual stimuli. Emerging research in non-invasive brain stimulation (NIBS) has vastly developed into a diversity of areas with promising potential. One of its clinical applications is the single-pulse transcranial magnetic stimulation (sTMS) applied over the occipital cortex which has been approved for treating migraine with aura, albeit limited evidence. Studies have also investigated other NIBS techniques, such as repetitive TMS (rTMS) and transcranial direct current stimulation (tDCS), for migraine prophylaxis but with conflicting results. As a dynamic brain disorder with widespread pathophysiology, targeting migraine with NIBS is challenging. Furthermore, unlike the motor cortex, evidence suggests that the visual cortex may be less plastic. Controversy exists as to whether the same fundamental principles of NIBS, based mainly on findings in the motor cortex, can be applied to the visual cortex. This review aims to explore existing literature surrounding NIBS studies on the visual system of migraine. We will first provide an overview highlighting the direct implication of the visual network in migraine. Next, we will focus on the rationale behind using NIBS for migraine treatment, including its effects on the visual cortex, and the shortcomings of currently available evidence. Finally, we propose a broader perspective of how novel approaches, the concept of brain networks and the integration of multimodal imaging with computational modeling, can help refine current NIBS methods, with the ultimate goal of optimizing a more individualized treatment for migraine.

13.
Prog Brain Res ; 255: 371-402, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33008514

RESUMO

Medication overuse headache (MOH), previously known as analgesic abuse headache or medication misuse headaches, is a common form of chronic headache disorder that has a detrimental impact on health and society. Although it has been widely accepted that overusing abortive medications is paradoxically the cause of MOH and drug discontinuation is the treatment of choice, ongoing debates exist as to whether drug consumption per se is the cause or consequence of headache chronification. Certain features in MOH such as their compulsive drug-seeking behavior, withdrawal headaches and high relapse rates share similarities with drug dependence, suggesting that there might be common underlying biological and psychobehavioral mechanisms. In this regard, this article will discuss the updated evidence and current debates on the possible biobehavioral overlap between MOH and drug dependence. To begin with, we will discuss whether MOH has characteristics of substance dependence based on standard psychiatry diagnostic criteria and other widely used dependence scales. Recent epidemiological studies underscoring common psychiatric comorbidities between the two disorders will also be presented. Although both demonstrate seemingly distinct personality traits, recent studies revealed similar decision-making impairment from a cognitive perspective, indicating the presence of a maladaptive reward system in both disorders. In addition, emerging imaging studies also support this notion by showing reversible morphological and functional brain changes related to the mesocorticolimbic reward circuitry in MOH, with a strong resemblance to those in addiction. Finally, an increased familial risk for drug dependence and genetic association with dopaminergic and drug dependence molecular pathways in MOH also support a possible link between MOH and addiction. Understanding the role of dependence in MOH will have a great impact on disease management as this will provide the missing piece of the puzzle in current therapeutic strategies.

14.
Prog Brain Res ; 255: 69-97, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33008516

RESUMO

Migraine and epilepsy share in many ways with regard to their clinical presentation and pathophysiological mechanisms. A state of central hyper-excitability or hyper-responsivity is fundamental in their pathophysiology. Antiepileptic drugs (AEDs) have long been used for migraine prevention, other than for seizure control. Evidence from numerous clinical trials has well demonstrated the therapeutic effects of some of the AEDs in this regard. In this paper, we will first discuss the clinical similarity between migraine and epilepsy. Then, to address the rationale of AEDs usage in migraine prevention, we focus on those electrophysiological studies conducted in patients with migraine demonstrating evidence of central hyper-excitability or hyper-responsivity. Although controversies remain, three common findings are derived from the literature: (1) cortical hyper-excitability, (2) impaired intra-cortical inhibition or increased intra-cortical facilitation, and (3) dis-habituation (hyper-responsivity). Each single feature may actually represent one of the different manifestations derived from an overall central excitation/inhibition imbalance. Finally, we review those studies addressing the therapeutic effects of AEDs and the associated alteration in central excitability. These studies provided direct evidence that effective prevention by AEDs is associated with the restoration of excitation/inhibition imbalance.

15.
Wei Sheng Yan Jiu ; 49(5): 724-730, 2020 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-33070812

RESUMO

OBJECTIVE: To identify the relationship between fever and diarrhea 2-week prevalence and Yingyangbao(YYB) effective consumption among infants and young children. METHODS: A total of 2952 infants and young children aged from 6 to 24 month in 10 impoverished counties of Henan province were selected by multi-stage random sampling between June and September 2017. To acquire 2-week prevalence information of infants and young children, their caregivers were investigated by self-made questionnaire. The structural equation model was utilized in multi-factor analysis. RESULTS: After adjusting potential confounders, YYB effective consumption reduced2-week prevalence of fever(ß=-0. 279, P=0. 001) and diarrhea(ß=-0. 182, P=0. 042) among infants and young children. Nutrition knowledge and YYB benefit cognition of caregivers reduced2-week prevalence of fever(γ=-0. 002, 95%CI-0. 004~-0. 001, P=0. 003) and diarrhea(γ=-0. 001, 95%CI-0. 003~0. 000, P=0. 049) indirectly through chain mediation path of "nutrition knowledge-YYB benfit cognition-YYB effective consumption-fever/diarrheal". CONCLUSION: YYB effective consumption can reduce 2-week prevalence of fever and diarrhea among infants and young children. Nutrition knowledge and YYB benefit cognition can improve YYB effective consumption and thus reduce 2-week prevalence of fever and diarrhea indirectly.


Assuntos
Suplementos Nutricionais , População Rural , Criança , Pré-Escolar , Diarreia/epidemiologia , Diarreia/prevenção & controle , Alimentos Fortificados , Humanos , Lactente , Prevalência
16.
Vision Res ; 178: 18-27, 2020 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-33075727

RESUMO

Holistic processing is a hallmark of face perception and has been observed separately for facial identity and expression. While the identity of a face remains constant regardless of any changes in facial expressions, to what extent is holistic processing of facial identity affected by task-irrelevant facial expressions? If holistic processing is flexible and integrates both identity and expression information, the magnitude of holistic processing of facial identity may be systematically modulated by different facial expressions, due to either visual or emotional differences among the expressions. In Experiment 1, participants matched the identities of target halves of two sequentially presented face composites, with both composites showing either positive (happy) or negative (angry) expressions. The presentation duration for the test composite was either short (200 ms) or long (until a response). With a short presentation duration, the magnitude of holistic processing of identity for happy and angry composites was comparable. In contrast, with a long presentation duration, holistic processing of identity was reduced for angry compared with happy face composites. Experiment 2 replicated the results and showed reduced holistic processing of identity for face composites with either angry or neutral expressions, compared with happy expressions, given a long presentation duration. Because the modulation of facial expressions on holistic processing of facial identity was observed with long, but not short, presentation durations, these results suggest that such influence is unlikely solely due to visual differences, but may instead arise from cognitive evaluation of the emotions conveyed by facial expressions.

17.
Eur J Immunol ; 2020 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-33080044

RESUMO

Despite the availability of antibiotics, current therapies to treat sepsis are still ineffective and many clinical trials aimed at neutralizing of specific inflammatory cytokines have failed, suggesting the urgent need for new treatments. Using two models of LPS-induced endotoxemia and cecal ligation and puncture (CLP)-induced sepsis, we investigated the effects of C1q/TNF-related protein 4(CTRP4) on septic lethality and sepsis-induced inflammation. The effects of CTRP4 on survival, inflammation, organ damage, and bacterial clearance were assessed. Here we found that CTRP4 decreased the mortalities of mice and alleviated pathological lung injury in mice model. In vivo depletion and adoptive transfer studies showed CTRP4-expressing macrophages as the key cell type inhibiting LPS-induced septic shock. The mechanism associated with the CTRP4 deficiency involved promoting of TLR4 internalization and activation of downstream pathways that resulted in a lethal, prolonged proinflammatory cytokine storm. Treatment of macrophages with exogenous CTRP4 abrogated proinflammatory cytokine production. Our results showed CTRP4 regulates inflammatory response and could be a promising strategy to treat septic shock. This article is protected by copyright. All rights reserved.

18.
Medicine (Baltimore) ; 99(42): e22709, 2020 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-33080722

RESUMO

BACKGROUND: Despite some researchers have compared the safety and effectiveness of percutaneous endoscopic discectomy (PED) and microendoscopic discectomy (MD) for the lumbar disc herniation; however, they got conflicting outcomes in several variables. Therefore, our aim was to clarify whether PED produces less surgical trauma and better clinical results than MD. METHODS: A single-center, retrospective cohort trial was conducted for the comparison of the safety and effectiveness between the MD and PED in the patients with lumbar disc herniation who received surgery from May 2016 to July 2018 in our hospital. The inclusion criteria for our investigation included:The follow-ups were performed 6 weeks, 3, 6, 12 and 24 months after the surgery. Numeric Rating Scale, Short-form 36, and Oswestry Disability Index, as well as complications were evaluated in our study. The software of SPSS Version 22.0 (IBM Corporation, Armonk, NY) was applied to analyze all the statistical data. When P is less than .05, the difference is significant in statistics. RESULTS: This protocol will provide a solid theoretical basis for exploring which technique is better in treatment of lumbar disc herniation. TRIAL REGISTRATION: This protocol was registered in Research Registry (researchregistry6005).

19.
Asian J Anesthesiol ; 58(1): 24-34, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-33081431

RESUMO

OBJECTIVE: Several studies have demonstrated increased postoperative mortality rates in patients on chronic hemodialysis compared with non-dialyzed patients. However, limited studies have examined factors that may contribute to postoperative mortality. METHODS: In this retrospective cohort study, data were collected from 9,140 dialysis and 45,725 non-dialysis patients undergoing surgery between 2007 to 2009 from Taiwan's National Health Insurance Registry Database. Patient demographics, comorbidities, and anesthesia duration were used to compare 30-day postoperative mortality differences in dialysis patients. RESULTS: Dialysis patients undergoing first-time surgery were significantly older, more likely male, and possessed more comorbidities. Overall, dialysis patients had significantly higher all-cause postoperative mortality (odds ratio, 15.005; 95% confidence interval, 11.917-18.893). Gender (hazard ratio [HR], 0.762), age (HR, 1.012), longer duration of inhalation general anesthesia (HR, 1.113), and comorbidities of hypertension (HR, 0.759), diabetes (HR, 1.339), congestive heart failure (HR, 1.232), coronary artery disease (HR, 1.326), cerebral vascular accident (HR, 1.312), intracranial hemorrhage (HR, 6.765), gastrointestinal bleeding (HR, 1.396), and liver cirrhosis (HR, 2.027), independently increased postoperative mortality risk in dialysis patients. Of the comorbidities, intracranial hemorrhage posed the greatest risk. CONCLUSION: Patient demographics, anesthesia factors, and comorbidities help dialysis patients understand their postoperative mortality. These potential risk factors also inform anesthesiologists and surgeons weight perioperative conditions in dialysis patients before surgery.

20.
Ann Palliat Med ; 2020 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-33081483

RESUMO

BACKGROUND: Influenza is the most prevalent acute respiratory infection worldwide. There are many different traditional Chinese medicine (TCM) therapies, which could reduce the duration of fever during influenza. However, there are no clinical practice guidelines (CPG) involving TCM therapies for influenza. Therefore, the present study aimed to establish a protocol for the development of CPG on TCM therapy for influenza. METHODS: The CPG will be developed according to the Institute of Medicine, the Appraisal of Guidelines for Research and Evaluation Ⅱ, and the World Health Organization (WHO) guideline handbook, and will provide recommendations based on systematic reviews. We have established a guideline working group (including a guideline steering group, a guideline development group, a guideline secretary group, and a system evaluation group), and will formulate clinical questions based on the population, intervention, comparison, and outcomes format. The recommendations will be formed via evidence search, syntheses, and the nominal group technique method. We will also consider patients' values or preferences, peer review results, and declarations of interest in the CPG. The CPG is registered on the International Practice Guidelines Registry Platform (registration no. IPGRP-2019CN044). RESULTS: The protocol will provide a roadmap to develop an evidence-based CPG for influenza treated by TCM systematically. These guidelines would be the first CPG on TCM therapy for influenza, based on the WHO Handbook for Guideline Development, and will provide the necessary evidence for treating influenza using TCM.

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