Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 311
Filtrar
1.
Arch Microbiol ; 204(6): 321, 2022 May 14.
Artigo em Inglês | MEDLINE | ID: mdl-35567648

RESUMO

Plant-microbe interactions affect ecosystem function, and plant species influence relevant microorganisms. However, the different genotypes of maize that shape the structure and function of the rhizosphere microbial community remain poorly investigated. During this study, the structures of the rhizosphere microbial community among three genotypes of maize were analyzed at the seedling and maturity stages using high-throughput sequencing and bioinformatics analysis. The results demonstrated that Tiannuozao 60 (N) showed higher bacterial and fungal diversity in both periods, while Junlong1217 (QZ) and Fujitai519 (ZL) had lower diversity. The bacterial community structure among the three varieties was significantly different; however, fewer differences were found in the fungal community. The bacterial community composition of N and QZ was similar yet different from ZL at the seedling stage. The bacterial networks of the three cultivars were more complex than the fungal networks, and the networks of the mature stages were more complex than those of the seedling stages, while the opposite was true for the fungi. FAPROTAX functional and FUNGuild functional predictions revealed that different varieties of maize were different in functional abundance at the genus level, and these differences were related to breeding characteristics. This study suggested that different maize genotypes regulated the rhizosphere bacterial and fungal communities, which would help guide practices.

2.
Zhongguo Zhong Yao Za Zhi ; 47(9): 2491-2499, 2022 May.
Artigo em Chinês | MEDLINE | ID: mdl-35531696

RESUMO

The present study investigated the therapeutic effect and mechanism of Di'ao Xinxuekang(DXXK) on non-alcoholic steatohepatitis(NASH) in mice. Sixty-five C57 BL/6 J mice were randomly divided into a normal group and an experimental group for model induction with the high-fat diet for 16 weeks. Then the mice in the experimental group were randomly divided into a model group, an atorvastatin group(4 mg·kg~(-1)·d~(-1)), and high-(200 mg·kg~(-1)·d~(-1)), medium-(60 mg·kg~(-1)·d~(-1)), and low-dose(20 mg·kg~(-1)·d~(-1)) DXXK groups, with 10 mice in each group. Drugs were administered by gavage for eight weeks. Serum lipid, liver lipid, serum alanine aminotransferase(ALT), aspartate aminotransferase(AST), malondialdehyde(MDA), superoxide dismutase(SOD), and glutathione reductase(GSH-Px) were determined. Interleukin-1ß(IL-1ß) and tumor necrosis factor-α(TNF-α) were measured by enzyme-linked immunosorbent assay(ELISA). The liver index was calculated. The liver pathological change and lipid accumulation were observed by HE and oil red O staining. The liver ultrastructure was observed by the transmission electron microscope. The mRNA and protein expression of nuclear factor-erythroid 2 related factor 2(Nrf2) and heme oxygenase-1(HO-1) was detected by real-time fluorescence-based quantitative PCR and Western blot, respectively. The results showed that compared with the normal group, the model group displayed serum lipid and liver lipid metabolism disorders, elevated transaminase, lipid deposition, steatosis, and inflammation, suggesting that the NASH model in mice was properly induced. Compared with the model group, the DXXK groups showed decreased serum lipid, liver lipid, ALT, AST, MDA, IL-1ß, and TNF-α, increased SOD and GSH-Px, alleviated hepatic steatosis, ballooning, and inflammation, and up-regulated Nrf2 and HO-1 gene and protein expression. In conclusion, DXXK can significantly alleviate NASH in mice, which is related to the inhibition of oxidative stress and inflammatory damage by up-regulating the Nrf2/HO-1 signaling pathway.


Assuntos
Fator 2 Relacionado a NF-E2 , Hepatopatia Gordurosa não Alcoólica , Animais , Medicamentos de Ervas Chinesas , Inflamação/metabolismo , Lipídeos , Fígado , Camundongos , Fator 2 Relacionado a NF-E2/genética , Fator 2 Relacionado a NF-E2/metabolismo , Hepatopatia Gordurosa não Alcoólica/tratamento farmacológico , Hepatopatia Gordurosa não Alcoólica/genética , Hepatopatia Gordurosa não Alcoólica/metabolismo , Estresse Oxidativo , Transdução de Sinais , Superóxido Dismutase/genética , Superóxido Dismutase/metabolismo , Fator de Necrose Tumoral alfa/metabolismo
4.
Artigo em Inglês | MEDLINE | ID: mdl-35435545

RESUMO

Gold (Au) nanoparticles supported on certain platforms display highly efficient activity on nitroaromatics reduction. In this study, steam-activated carbon black (SCB) was used as a platform to fabricate Au/SCB composites via a green and simple method for 4-nitrophenol (4-NP) reduction. The obtained Au/SCB composites exhibit efficient catalytic performance in reduction of 4-NP (rate constant kapp = 2.1925 min-1). The effects of SCB activated under different steam temperature, Au loading amount, pH, and reaction temperature and NaBH4 concentration were studied. The structural advantages of SCB as a platform were analyzed by various characterizations. Especially, the result of N2 adsorption-desorption method showed that steam activating process could bring higher surface area (from 185.9689 to 249.0053 m2/g), larger pore volume (from 0.073268 to 0.165246 cm3/g), and more micropore for SCB when compared with initial CB, demonstrating the suitable of SCB for Au NP anchoring, thus promoting the catalytic activity. This work contributes to the fabrication of other supported metal nanoparticle catalysts for preparing different functional nanocomposites for different applications.

5.
J Vis Exp ; (181)2022 03 25.
Artigo em Inglês | MEDLINE | ID: mdl-35404349

RESUMO

Chemotaxis identification is very important for the research and application of rhizosphere growth-promoting bacteria. We established a straightforward method to quickly identify the chemoattractants that could induce the chemotactic movement of rhizosphere growth-promoting bacteria on sterile glass slides via simple steps. Bacteria solution (OD600 = 0.5) and sterile chemoattractant aqueous solution were added dropwise on the glass slide at an interval of 1 cm. An inoculating loop was used to connect the chemoattractant aqueous solution to the bacterial solution. The slide was kept at room temperature for 20 min on the clean bench. Finally, the chemoattractant aqueous solution was collected for bacterial counting and microscopic observation. In this study, through multiple comparisons of experimental results, the method overcame multiple shortcomings of traditional bacterial chemotaxis methods. The method reduced the error of plate counting and shortened the experimental cycle. For the identification of chemoattractant substances, this new method can save 2-3 days compared to the traditional method. Additionally, this method allows any researcher to systematically complete a bacterial chemotaxis experiment within 1-2 days. The protocol can be considered a valuable resource for understanding plant-microbe interactions.


Assuntos
Fatores Quimiotáticos , Quimiotaxia , Bactérias , Fatores Quimiotáticos/farmacologia , Exsudatos e Transudatos , Raízes de Plantas/microbiologia
6.
J Healthc Eng ; 2022: 7246848, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35463676

RESUMO

Objective: Sarcopenia is one of the influencing factors of poor prognosis in patients with gastric cancer but the association with readmission are unknown. We aimed to explore factors associated with readmission after gastrectomy and to determine whether preoperative sarcopenia is a common outcome in readmitted patients. Methods: In this case-control study, patients who underwent gastric resection in the First Affiliated Hospital of Wenzhou Medical University between April 2016 and September 2017 were included. The reasons of readmission patients were described. The readmission patients and non-readmission patients were matched by propensity score matching (PSM). The univariate analysis was applied for the baseline characteristics, operative details, postoperative prognosis and discharge disposition, and multiple logistic regression analysis for the independent risk factors of readmission. Results: The unplanned readmission rate within 30 days of radical gastrectomy for gastric cancer was 6.5% (43/657). The average time interval from discharge to readmission was 13 days. Delayed gastric evacuation was the main cause of readmission (18.6%, 8/43). Body mass index (BMI), nutritional risk screening (NRS) 2002 score, history of abdominal surgery, sarcopenia, and preoperative albumin were included in the multivariate logistic regression analysis. NRS 2002 (OR = 3.43, 95% CI: 1.10-10.72, P=0.034) and sarcopenia (OR = 4.25, 95% CI: 1.13-16.02, P=0.033) were found to be independently associated with unplanned readmission within 30 days of radical gastrectomy for cancer. Other factors such as age, sex, BMI, American Society of Anesthesiologists grade, surgical method, operation and reconstruction type, TNM stage, surgical duration, previous abdominal surgery, and preoperative albumin and hemoglobin level were not associated with unplanned readmission after radical gastrectomy for cancer. Conclusions: Sarcopenia and low nutritional status are independently associated with unplanned readmission within 30 days of radical gastrectomy for cancer.


Assuntos
Sarcopenia , Neoplasias Gástricas , Albuminas , Estudos de Casos e Controles , Gastrectomia/efeitos adversos , Humanos , Estado Nutricional , Readmissão do Paciente , Complicações Pós-Operatórias , Sarcopenia/complicações , Sarcopenia/cirurgia , Neoplasias Gástricas/complicações , Neoplasias Gástricas/cirurgia
7.
J Immunol ; 208(8): 1924-1936, 2022 04 15.
Artigo em Inglês | MEDLINE | ID: mdl-35365563

RESUMO

The germinal center (GC) response is essential for generating memory B and long-lived Ab-secreting plasma cells during the T cell-dependent immune response. In the GC, signals via the BCR and CD40 collaboratively promote the proliferation and positive selection of GC B cells expressing BCRs with high affinities for specific Ags. Although a complex gene transcriptional regulatory network is known to control the GC response, it remains elusive how the positive selection of GC B cells is modulated posttranscriptionally. In this study, we show that methyltransferase like 14 (Mettl14)-mediated methylation of adenosines at the position N 6 of mRNA (N 6-methyladenosine [m6A]) is essential for the GC B cell response in mice. Ablation of Mettl14 in B cells leads to compromised GC B cell proliferation and a defective Ab response. Interestingly, we unravel that Mettl14-mediated m6A regulates the expression of genes critical for positive selection and cell cycle regulation of GC B cells in a Ythdf2-dependent but Myc-independent manner. Furthermore, our study reveals that Mettl14-mediated m6A modification promotes mRNA decay of negative immune regulators, such as Lax1 and Tipe2, to upregulate genes requisite for GC B cell positive selection and proliferation. Thus, our findings suggest that Mettl14-mediated m6A modification plays an essential role in the GC B cell response.


Assuntos
Linfócitos B , Centro Germinativo , Metiltransferases , Adenosina/metabolismo , Animais , Linfócitos B/metabolismo , Linfócitos B/fisiologia , Proliferação de Células , Centro Germinativo/metabolismo , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Metilação , Metiltransferases/genética , Metiltransferases/metabolismo , Camundongos
8.
Int J Biol Sci ; 18(5): 2060-2074, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35342361

RESUMO

Although cisplatin is the most effective first-line drug in the management of advanced non-small cell lung cancer (NSCLC), drug resistance remains a major clinical challenge. There is increasing evidence that icariside II (IS) exhibits antitumour activity in a variety of cancers. In the current study, we investigated the anticancer effects of icariside II combined with cisplatin and elucidated the underlying mechanism in NSCLC. Here, we showed that cotreatment with IS and cisplatin inhibited cell proliferation and induced cellular apoptosis. Using mRNA sequencing (mRNA-seq), we identified differentially expressed genes (DEGs) in which there was an enrichment in PERK-mediated unfolded protein response (UPR) signalling. The western blot results revealed that IS activated endoplasmic reticulum (ER) stress, including three branches of UPR signalling, PERK, IRE1 and ATF6, and the downstream PERK-eIF2α-ATF4-CHOP pathway, thus potentiating the apoptosis induced by cisplatin. In addition, the combination of IS with cisplatin significantly reduced xenograft tumour growth in C57BL/6 and BALB/c nude mice in vivo. Notably, the combination therapy displayed no evident toxicity. Taken together, IS enhances cisplatin-induced apoptosis partially by promoting ER stress signalling in NSCLC, suggesting that combination treatment with IS and cisplatin is a novel potential therapeutic strategy for NSCLC.


Assuntos
Carcinoma Pulmonar de Células não Pequenas , Neoplasias Pulmonares , Animais , Apoptose , Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico , Linhagem Celular Tumoral , Cisplatino/farmacologia , Cisplatino/uso terapêutico , Estresse do Retículo Endoplasmático , Flavonoides , Humanos , Neoplasias Pulmonares/tratamento farmacológico , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Nus , RNA Mensageiro , eIF-2 Quinase/genética , eIF-2 Quinase/metabolismo
9.
Horm Metab Res ; 54(4): 259-267, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35255519

RESUMO

Zinc-α2-glycoprotein (ZAG) is an adipokine involved in body metabolism, and now it has been shown to be present in the brain and play a role in some neurological diseases such as epilepsy and Alzheimer's disease. In the present study, we employed ZAG knockout (KO) mice to investigate the effects of ZAG on behaviors after fasting and in vitro used overexpression (OV) ZAG in HT-22 cells to further clarify the possibly underlying mechanism. The results showed that ZAG exists widely in the brain tissues of mice and significantly increased during fasting. In ZAG KO group the depression-like behaviors were significantly increased after fasting for 24 hours, meanwhile the hippocampal reactive oxygen species (ROS) content was significantly increased. In vitro, serum deprivation led to the increasing of neuronal death and ROS, the reduced mitochondrial membrane potential and ATP levels, while ZAG overexpression alleviated these negative effects. The ß3 adrenoreceptor (ß3AR)/protein kinase A (PKA)/cAMP response element-binding (CREB) pathway possibly mediated the effects of ZAG on antioxidation. These results proposed a possible target for novel therapeutic approaches to the treatment of depression and provide potential link between adipose tissue and psychiatric disease.


Assuntos
Adipocinas , Depressão , Animais , Hipocampo/metabolismo , Camundongos , Camundongos Knockout , Estresse Oxidativo , Espécies Reativas de Oxigênio , Proteínas de Plasma Seminal/metabolismo
10.
Lipids ; 57(3): 153-162, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35262212

RESUMO

Oxidative stress is an important factor in the occurrence and development of liver disease. Medium-chain fatty acids (MCFAs) have potential antioxidant function, whereas the exact underlying mechanism of MCFA in oxidative injury of hepatocytes remains unclear. In our present study, three different MCFAs, 8-carbon octanoic acid (OA), 10-carbon capric acid (CA), and 12-carbon lauric acid (LA), have been performed to observe their protective action for hepatocyte under the H2 O2 challenge. The result showed that MCFA treatment significantly increased the cell viability, T-AOC, and expression of antioxidant-related genes in AML12 cells under oxidative stress condition, and reduced reactive oxygen species (ROS) production. Moreover, MCFA treatment significantly increased the protein expression of Nrf2 and the phosphorylation level of ERK1/2; LA treatment significantly promoted the Nrf2 nuclear translocation. With a further test, the rescue ability of MCFA was blocked by treating with the ERK inhibitor U0126. Overall, our data suggested that MCFA treatment has positive impact on protecting AML12 cells against oxidative stress through ERK1/2/Nrf2 pathway.


Assuntos
Antioxidantes , Fator 2 Relacionado a NF-E2 , Antioxidantes/metabolismo , Antioxidantes/farmacologia , Carbono , Sistema de Sinalização das MAP Quinases , Fator 2 Relacionado a NF-E2/genética , Fator 2 Relacionado a NF-E2/metabolismo , Estresse Oxidativo , Espécies Reativas de Oxigênio/metabolismo
11.
Opt Lett ; 47(6): 1395-1398, 2022 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-35290322

RESUMO

The two-dimensional continuous wavelet transform (2D CWT) has been widely used in single-shot fringe projection profilometry (FPP) because of its multi-resolution characteristics and anti-noise ability. This Letter aims to develop a novel 2D wavelet transform model in FPP. Dual-angle rotation operation on a 2D wavelet is performed to improve the directional selectivity and frequency localization of the wavelet. The proposed method improves the accuracy of the phase calculation of wavelet transform profilometry (WTP) for higher accuracy reconstruction from the single-shot fringe. We present mathematical expressions, theoretical analysis, and the comparison to traditional 2D WTP. The experiments demonstrate the effectiveness of the proposed method.

12.
Front Oncol ; 12: 851091, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35311068

RESUMO

Objective: Malnutrition is recognized as a risk factor for poor outcome in patients with gastric cancer (GC). In 2018, the Global Leadership Initiative on Malnutrition (GLIM) published standardized criteria for the diagnosis of malnutrition. Our aim was to investigate whether any of the components of the GLIM diagnostic criteria were related to worse clinical outcomes in patients with GC. Methods: This study analyzed patients with GC who underwent radical gastrectomy in our hospital between 2014 and 2019. A preoperative nutritional assessment was performed for each patient. Matching was based on the presence of three GLIM components: high weight loss (WL), low body mass index (BMI), and low skeletal muscle index (SMI). Results: The analysis included 1,188 patients, including 241 (20.3%) with high WL, 156 (13.1%) with low BMI, and 355 (29.9%) with low SMI. Before matching, patients who met the GLIM component criteria were mostly associated with older age, low nutritional reserves, and late tumor progression. After matching, the clinical characteristics of the three cohorts were balanced. In the matched queue, the survival prognosis of the high WL group was worse than that of the non-WL group, and the postoperative complication rate was higher in the low SMI group than in the normal SMI group (P <0.05). In addition, the clinical outcomes in the low and normal BMI groups were similar (P >0.05). Conclusion: Of the GLIM criteria, high WL and low SMI may be associated with poor clinical outcomes in patients with GC, while a low BMI may not be associated with outcome.

13.
Trials ; 23(1): 171, 2022 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-35193665

RESUMO

BACKGROUND: The prevalence rate of hypertension in the Chinese population is on the rise, and the control rate of hypertension is low. International guidelines, including the 2018 Chinese Guidelines for the Management of Hypertension, recommend optimized drug selection and combination therapy for patients with stage 2 hypertension and blood pressure ≥ 160/100 mmHg, including valsartan/amlodipine (Val/Aml). The traditional Chinese medicine (TCM) compound Tengfu Jiangya tablet (TJT; No. Z20110021, Shandong Provincial Food and Drug Administration) is prepared in the medical institution of Affiliated Hospital of Shandong University of Traditional Chinese Medicine. It is an effective compound preparation of TCM for the treatment of hypertension in the national clinical research base of TCM. The aim of this study was to evaluate the efficacy and safety of TJT combined with Val/Aml in the treatment of stage 2 hypertension with hyperactivity of liver yang. METHODS: This randomized double-blind, placebo-controlled, multicenter trial will be conducted with a total of 288 participants with stage 2 hypertension at seven clinical trial centers. The stratified random method will be used, and the subcenter will be taken as the stratification factor. Eligible patients will be randomly assigned (1:1) into groups receiving either TJT or placebo three times daily for 28 days, both combined with Val/Aml 80/5 mg. The primary efficacy endpoint is the reduction in the mean sitting systolic blood pressure (msSBP) and the mean sitting diastolic blood pressure (msDBP) from baseline to week 4. Adverse events and laboratory test results will be monitored throughout the trial. DISCUSSION: This is the first placebo-controlled randomized trial conducted to evaluate the efficacy and safety of a Chinese herbal extract combined with Val/Aml in patients with stage 2 hypertension. Our study may help to provide evidence-based recommendations of a complementary preventive measure for stage 2 hypertension. TRIAL REGISTRATION: Chinese Clinical Trial Registry ChiCTR2000030611 . Registered on 8 March 2020.


Assuntos
Quimioterapia Combinada , Medicamentos de Ervas Chinesas , Hipertensão , Anlodipino/efeitos adversos , Anti-Hipertensivos/efeitos adversos , Pressão Sanguínea , Método Duplo-Cego , Quimioterapia Combinada/efeitos adversos , Medicamentos de Ervas Chinesas/efeitos adversos , Humanos , Hipertensão/tratamento farmacológico , Estudos Multicêntricos como Assunto , Ensaios Clínicos Controlados Aleatórios como Assunto , Comprimidos , Valsartana/efeitos adversos
14.
Adv Mater ; 34(16): e2108939, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35181956

RESUMO

Large-area fabrication of perovskite light-emitting diodes (PeLEDs) through mass-production techniques has attracted growing attention due to their potential applications in lighting. Several breakthroughs are made for red/infrared and green emissions. Nevertheless, large-area blue/sky-blue PeLEDs, a requisite color for lighting, have not yet been reported. Here, efficient and large-area sky-blue PeLEDs are fabricated through blade-coating supersaturated precursors. The volume ratio of dimethyl sulfoxide to dimethylformamide is tuned to obtain a supersaturated CsPb(Br0.84 Cl0.16 )3 solution. Blade-coating this supersaturated precursor results in nucleation in the solution phase with much higher nucleation sites, and a faster crystallization rate. The uniform films formed by this approach exhibit smaller grain size, lower trap density, and higher radiative recombination rate. The peak external quantum efficiency of the blade-coated PeLEDs reaches 10.3% with sky-blue emission (489 nm). Benefitting from the robustness of this blade-coating technique, large-area sky-blue PeLEDs with a device area of 28 cm2 are also achieved with uniform emission. This work represents a significant step forward toward flat-panel lighting and full-color display for the PeLEDs.

15.
Int J Mol Sci ; 23(3)2022 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-35163196

RESUMO

Inflammatory responses have been shown to induce hyperglycemia, yet the underlying mechanism is still largely unclear. GLP-1 is an important intestinal hormone for regulating glucose homeostasis; however, few studies have investigated the influence of digestive tract Salmonella infection on enteroendocrine L cell secretions. In this study, we established a model of Salmonella-infected piglets by oral gavage in order to analyze the effects of Salmonella infection on enteroendocrine L cell function. Furthermore, in vitro lipopolysaccharide (LPS) was administered to STC-1 cells to clarify its direct effect on GLP-1 secretion. The results showed that significantly increased blood glucose in the group of Salmonella-infected piglets was observed, and Salmonella infection decreased blood GLP-1 content. Then, ileal epithelium damage was observed by histological detection, and this was further verified by TUNEL staining. We identified activation of TLR signaling demonstrating up-regulated expressions of TLR4 and nuclear factor-kappa B (NF-ΚB). Furthermore, it was shown that Salmonella induced pyroptosis of enteroendocrine L cells and enhanced the secretion of IL-1ß through augmenting gene and protein expressions of NOD-like receptor protein 3 (NLRP3), apoptosis-associated speck-like protein containing a carboxyl-terminal CARD (ASC), Caspase 1, and gasdermin D (GSDMD). Meanwhile, in vitro LPS treatment induced the pyroptosis of STC-1 cells and reduced the secretion of GLP-1. Altogether, the results demonstrated that Salmonella infection can reduce secretion of GLP-1 by inducing pyroptosis of intestinal L cells, which may eventually result in hyperglycemia. The results provided evidence for the cause of hyperglycemia induced by inflammation and shed new light on glucose homeostasis regulation.


Assuntos
Peptídeo 1 Semelhante ao Glucagon/metabolismo , Hiperglicemia/etiologia , Salmonelose Animal/metabolismo , Animais , Caspase 1/metabolismo , China , Células Enteroendócrinas/citologia , Células Enteroendócrinas/metabolismo , Hiperglicemia/patologia , Inflamassomos/metabolismo , Inflamação , Células L/metabolismo , Camundongos , NF-kappa B/metabolismo , Proteína 3 que Contém Domínio de Pirina da Família NLR/metabolismo , Piroptose/efeitos dos fármacos , Piroptose/fisiologia , Salmonella/patogenicidade , Transdução de Sinais , Suínos/microbiologia
16.
Cell Rep ; 38(6): 110338, 2022 02 08.
Artigo em Inglês | MEDLINE | ID: mdl-35139388

RESUMO

The spliceosome is a large ribonucleoprotein complex responsible for pre-mRNA splicing and genome stability maintenance. Disruption of the spliceosome activity may lead to developmental disorders and tumorigenesis. However, the physiological role that the spliceosome plays in B cell development and function is still poorly defined. Here, we demonstrate that ubiquitin-specific peptidase 39 (Usp39), a spliceosome component of the U4/U6.U5 tri-snRNP complex, is essential for B cell development. Ablation of Usp39 in B cell lineage blocks pre-pro-B to pro-B cell transition in the bone marrow, leading to a profound reduction of mature B cells in the periphery. We show that Usp39 specifically regulates immunoglobulin gene rearrangement in a spliceosome-dependent manner, which involves modulating chromatin interactions at the Igh locus. Moreover, our results indicate that Usp39 deletion reduces the pre-malignant B cells in Eµ-Myc transgenic mice and significantly improves their survival.


Assuntos
Linfócitos B/citologia , Genes de Imunoglobulinas/genética , Precursores de RNA/metabolismo , Spliceossomos/metabolismo , Proteases Específicas de Ubiquitina/genética , Animais , Humanos , Camundongos , Ribonucleoproteína Nuclear Pequena U4-U6/genética , Ribonucleoproteína Nuclear Pequena U5/metabolismo , Proteases Específicas de Ubiquitina/metabolismo
17.
Environ Sci Pollut Res Int ; 29(22): 33218-33229, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35022983

RESUMO

To explore the relationship between perinatal exposure to endocrine-disrupting chemicals and the male reproductive system of F3 generation, and to evaluate the toxicological effects of endocrine-disrupting chemicals on the reproductive system of F3 generation male rodents. PubMed and Web of Science databases were searched to obtain the studies; overall risk ratios (RRs) with 95% confidence intervals (95% CIs) were used to evaluate the relationship between exposure to endocrine-disrupting chemicals and reproductive system damage in F3 generation male rodents. Nine studies were included for analysis. Endocrine-disrupting chemicals are significantly associated with the reproductive system of male rodents of F3 generation, especially the testis (RR = 3.13, 95% CI: 2.05, 4.76), prostate (RR = 2.26, 95% CI: 1.27, 4.00), and kidney (RR = 2.83, 95% CI: 1.77, 4.52), but the current analysis does not prove that EDCs are the adverse factors for puberty abnormalities. The results indicated that the overall associations between atrazine (RR = 3.06, 95% CI: 1.10, 8.51, P = 0.032), DDT (RR = 6.26, 95% CI: 1.56, 25.08, P = 0.010), pesticide and insect repellent mixture (permethrin and DEET) (RR = 2.23, 95% CI: 1.34, 3.69, P = 0.002), and vinclozolin (RR = 4.71, 95% CI: 2.74, 8.10, P = 0.000) and reproductive system damage in F3 generation male rodents were statistically significant. Our study indicated that EDCs have an atavistic effect on the male reproductive system, and we should pay attention to the long-term effects of environmental exposure to endocrine disruptors in future generations.


Assuntos
Disruptores Endócrinos , Praguicidas , Animais , Disruptores Endócrinos/toxicidade , Exposição Ambiental , Feminino , Masculino , Gravidez , Roedores , Testículo
18.
Inorg Chem ; 61(4): 2076-2085, 2022 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-35025489

RESUMO

A novel binuclear ruthenium-based polyoxometalate, K6H[{Ru2Cl(H2O)(CH3COO)2}{WO(H2O)}2(PW9O34)2]·14H2O (1), was successfully synthesized by the conventional hydrothermal method. Compound 1 was well-characterized by single-crystal X-ray diffraction, X-ray powder diffraction (PXRD), infrared (IR) spectroscopy, X-ray photoelectron spectroscopy (XPS), electrospray ionization-mass spectrometry (ESI-MS), thermogravimetric analyses (TGA), and elemental analysis. The structural unit of compound 1 contains two [A-α-PW9O34]9- building blocks at the upper and lower positions connected by two W atoms and two Ru atoms, where the W atoms and Ru atoms are arranged in a trapezoidal arrangement and the Ru atoms are bridged by acetic acid. Furthermore, compound 1 features characteristic absorption bands in the visible region, which allows the investigation of its photocatalytic properties in visible light. Under simulated sunlight radiation (λ > 400 nm), compound 1 exhibits high photocatalytic activity and good circularity toward the oxidative coupling of amines to imines at room temperature with O2 as the sole oxidant.

19.
Am J Perinatol ; 2022 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-35016248

RESUMO

OBJECTIVE: Meconium is a common finding in amniotic fluid and placental specimens, particularly in term and post-term pregnancies. The objective of this paper was to perform a meta-analysis to examine the impact of endotracheal suctioning on the occurrence of meconium aspiration syndrome (MAS), mortality, and complications. STUDY DESIGN: PubMed, EMBASE, and the Cochrane library were systematically searched for comparative studies. Odds ratios (ORs), weighted mean differences (WMDs), and corresponding 95% confidence intervals (CIs) were used to compare the outcomes. RESULTS: Twelve studies were included in the meta-analysis. There were no significant impacts of endotracheal suctioning on the occurrence of MAS (OR = 3.05, 95% CI: 0.48-19.56), mortality (OR = 1.25, 95% CI: 0.35-4.44), the need for mechanical ventilation (OR = 4.20, 95% CI: 0.32-54.72), the occurrence of pneumothorax (OR = 0.99, 95% CI: 0.34-2.85), persistent pulmonary hypertension of the newborn (PPHN), (OR = 1.31, 95% CI: 0.58-2.98), hypoxic-ischemic encephalopathy (HIE) (OR = 0.82, 95% CI: 0.52-1.30), and length of stay (WMD = -0.11, 95% CI: -0.99-0.77). CONCLUSION: Routine endotracheal suctioning at birth is not useful in preventing MAS, mortality, mechanical ventilation, PPHN, HIE, and prolonged length of stay in neonates born through MSAF. KEY POINTS: · Routine suctioning is not recommended for newborns.. · Endotracheal aspiration is not beneficial for MAS.. · Future research may focus on selected neonates..

20.
Sci Rep ; 12(1): 648, 2022 01 13.
Artigo em Inglês | MEDLINE | ID: mdl-35027603

RESUMO

Human activities have increased the input of nitrogen (N) to forest ecosystems and have greatly affected litter decomposition and the soil environment. But differences in forests with different nitrogen deposition backgrounds. To better understand the response of litter decomposition and soil environment of N-limited forest to nitrogen deposition. We established an in situ experiment to simulate the effects of N deposition on soil and litter ecosystem processes in a Picea schrenkiana forest in the Tianshan Mountains, China. This study included four N treatments: control (no N addition), low N addition (LN: 5 kg N ha-1 a-1), medium N addition (MN: 10 kg N ha-1 a-1) and high N addition (HN: 20 kg N ha-1 a-1). Our results showed that N addition had a significant effect on litter decomposition and the soil environment. Litter mass loss in the LN treatment and in the MN treatment was significantly higher than that in the control treatment. In contrast, the amount of litter lost in the HN treatment was significantly lower than the other treatments. N application inhibited the degradation of lignin but promoted the breakdown of cellulose. The carbon (C), N, and phosphorus (P) contents of litter did not differ significantly among the treatments, but LN promoted the release of C and P. Our results also showed that soil pH decreased with increasing nitrogen application rates, while soil enzyme activity showed the opposite trend. In addition, the results of redundancy analysis (RDA) and correlation analyses showed that the soil environment was closely related to litter decomposition. Soil enzymes had a positive effect on litter decomposition rates, and N addition amplified these correlations. Our study confirmed that N application had effects on litter decomposition and the soil environment in a N-limited P. schrenkiana forest. LN had a strong positive effect on litter decomposition and the soil environment, while HN was significantly negative. Therefore, increased N deposition may have a negative effect on material cycling of similar forest ecosystems in the near future.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...