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1.
World J Stem Cells ; 12(8): 776-786, 2020 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-32952858

RESUMO

Mesenchymal stem cells (MSCs) have been widely exploited as promising candidates in clinical settings for bone repair and regeneration in view of their self-renewal capacity and multipotentiality. However, little is known about the mechanisms underlying their fate determination, which would illustrate their effectiveness in regenerative medicine. Recent evidence has shed light on a fundamental biological role of autophagy in the maintenance of the regenerative capability of MSCs and bone homeostasis. Autophagy has been implicated in provoking an immediately available cytoprotective mechanism in MSCs against stress, while dysfunction of autophagy impairs the function of MSCs, leading to imbalances of bone remodeling and a wide range of aging and degenerative bone diseases. This review aims to summarize the up-to-date knowledge about the effects of autophagy on MSC fate determination and its role as a stress adaptation response. Meanwhile, we highlight autophagy as a dynamic process and a double-edged sword to account for some discrepancies in the current research. We also discuss the contribution of autophagy to the regulation of bone cells and bone remodeling and emphasize its potential involvement in bone disease.

2.
Plants (Basel) ; 9(8)2020 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-32823635

RESUMO

Oaks (Quercus L.) are ideal models to assess patterns of plant diversity. We integrated the sequence data of five chloroplast and two nuclear loci from 50 Chinese oaks to explore the phylogenetic framework, evolution and diversification patterns of the Chinese oak's lineage. The framework phylogeny strongly supports two subgenera Quercus and Cerris comprising four infrageneric sections Quercus, Cerris, Ilex and Cyclobalanopsis for the Chinese oaks. An evolutionary analysis suggests that the two subgenera probably split during the mid-Eocene, followed by intergroup divergence within the subgenus Cerris around the late Eocene. The initial diversification of sections in the subgenus Cerris was dated between the mid-Oligocene and the Oligocene-Miocene boundary, while a rapid species radiation in section Quercus started in the late Miocene. Diversification simulations indicate a potential evolutionary shift on section Quercus, while several phenotypic shifts likely occur among all sections. We found significant negative correlations between rates of the lineage diversification and phenotypic turnover, suggesting a complex interaction between the species evolution and morphological divergence in Chinese oaks. Our infrageneric phylogeny of Chinese oaks accords with the recently proposed classification of the genus Quercus. The results point to tectonic activity and climatic change during the Tertiary as possible drivers of evolution and diversification in the Chinese oak's lineage.

3.
Gynecol Endocrinol ; 36(10): 929-933, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32223457

RESUMO

Background: A considerable proportion of pediatric disease burden is mainly caused by inborn errors of metabolism. Succinic semi-aldehyde dehydrogenase (SSADH) deficiency is an unusual disorder of the gamma-aminobutyric acid metabolism. Till date, very few cases have been reported in China.Case presentation: Trio-WES was used to characterize the ALDH5A1 gene in two children of a Chinese family, who presented with seizures, psychomotor delay, development regression, borderline cognition, hypotonia, and harbored the compound heterozygotes NM_001080.3: c.1321G > A (p. Gly441Arg) and c.727_735del (p. Leu243_Ser245del). The former has been reported earlier (rs1041467895), whereas the latter is novel. Amino acid coding at highly conserved amino acid residues was observed to be altered by both mutations. This structural impairment influenced the enzyme structure as indicated by the in silico protein modeling. Cerebral magnetic resonance imaging of the proband and her brother showed excessive gap in the cerebrum and abnormal signals in the bilateral frontal lobe, bilateral basal ganglia, and cerebral foot. Elevated levels of Gamma-hydroxybutyric aciduria were found in their patients on urine organic acid analysis.Conclusion: Our findings contribute to the current knowledge of missense and deletion mutations associated with SSADH deficiency.

4.
Front Genet ; 10: 965, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31632447

RESUMO

Effectively identifying the genetic structure and related factors of a species can facilitate understanding the evolutionary history of the species. Phylogeographic patterns and genetic data are essential in investigating the species historical processes and diversification that response to environmental, climatic and geological influences. In this study, Specific Length Amplified Fragment Sequencing (SLAF-seq) data and ecological niche models (ENMs) are combined to identify the genetic structure and demographic modeling of Quercus spinosa, and evaluate the impacts of historical range shifts, climatic variation, and landscape factors on this species. The population topology and genetic divergence of the Cenozoic were inferred by a site frequency spectrum based composite-likelihood approach which is a novel strategy for maximizing the utility of linked SLAF markers. The overall genetic structure using model-based and model-free clustering methods was consistently identified as two geographically distinct genetic clusters. A deep divergence between two natural lineages (i.e., a western Himalaya-Hengduan Mountains lineage and an eastern Qin-ling Mountains lineage) was observed. The demographic modeling and Niche reconstruction indicated that the two groups were diverged in the late Miocene and then presented as two distinct genetic lineages. With the Quaternary glacial climate fluctuation, two groups had continuous asymmetrical secondary contact and gene exchange in the Sichuan Basin during the last glacial maximum. Besides, a significant relationship between genetic distance and geography in all individuals was identified by the Mantel test. Overall, this study 1) contributes to a better understanding of the role played by Quaternary climatic fluctuation in the present-day distributions of Q. spinosa; 2) provides a comprehensive view of the genome-wide variation of sclerophyllous forests in ecological adaptive evolution; 3) indicates that dispersal limitation and ecological divergence contribute to the genome-wide differentiation of Q. spinosa, which supports a hypothesis that complex geography and climatic changes strongly influence the evolutionary origin and history of the species.

5.
Cell Signal ; 56: 1-14, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30465826

RESUMO

As the most commonly occurring form of primary renal tumor, renal cell carcinoma (RCC) is a malignancy accompanied by a high mortality rate. 3-phosphoinositide-dependent protein kinase 1 (PDK1) has been established as a protein target and generated considerable interest in both the pharmaceutical and academia industry. The aim of the current study was to investigate the effect of si-PDK1 on the RCC cell apoptosis, proliferation, migration, invasion and epithelial mesenchymal transition (EMT) in connection with the PI3K-PDK1-Akt pathway. Microarray analysis from the GEO database was adopted to identify differentially expressed genes (DEGs) related to RCC, after which the positive expression of the PDK1 protein in tissue was determined accordingly. The optimal silencing si-RNA was subsequently selected and RCC cell lines 786-O and A498 were selected and transfected with either a si-PDK1 or activator of the PI3K-PDK1-Akt pathway for grouping purposes. The mRNA and protein expressions of PDK1, the PI3K-PDK1-Akt pathway-, EMT- and apoptosis-related genes were then evaluated. The effect of si-PDK1 on cell proliferation, apoptosis, invasion and migration was then analyzed. Through microarray analysis of GSE6344, GSE53757, GSE14762 and GSE781, PDK1 was examined. PDK1 was determined to be highly expressed in RCC tissues. Si-PDK1 exhibited marked reductions in relation to the mRNA and protein expression of PDK1, PI3K, AKT as well as Vimentin while elevated mRNA and protein expressions of E-cadherin were detected, which ultimately suggested that cell migration, proliferation and invasion had been inhibited coupled with enhanced levels of cell apoptosis. While a notable observation was made highlighting that the PI3K-PDK1-Akt pathway antagonized the effect of PDK1 silencing. Taken together, the key observations of this study provide evidence suggesting that high expressions of PDK1 are found in RCC, while highlighting that silencing PDK1 could inhibit RCC cell proliferation, migration, invasion and EMT by repressing the PI3K-PDK1-Akt pathway.


Assuntos
Proteínas Quinases Dependentes de 3-Fosfoinositídeo/metabolismo , Carcinoma de Células Renais/patologia , Movimento Celular/genética , Proliferação de Células/genética , Transição Epitelial-Mesenquimal/genética , Neoplasias Renais/patologia , Proteínas Quinases Dependentes de 3-Fosfoinositídeo/biossíntese , Proteínas Quinases Dependentes de 3-Fosfoinositídeo/genética , Adulto , Idoso , Antígenos CD/metabolismo , Apoptose/genética , Caderinas/metabolismo , Pontos de Checagem do Ciclo Celular/genética , Linhagem Celular Tumoral , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Invasividade Neoplásica/genética , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas Proto-Oncogênicas c-akt/antagonistas & inibidores , Proteínas Proto-Oncogênicas c-akt/metabolismo , Interferência de RNA , RNA Interferente Pequeno/genética , Vimentina/metabolismo
6.
Eur Neurol ; 78(3-4): 200-209, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28898869

RESUMO

BACKGROUND/AIMS: The topological organization of brain functional networks is impaired in Parkinson's disease (PD). However, the altered patterns of functional network hubs in different subtypes of PD are not completely understood. METHODS: 3T resting-state functional MRI and voxel-based graph-theory analysis were employed to systematically investigate the intrinsic functional connectivity patterns of whole-brain networks. We enrolled 31 patients with PD (12 tremor dominant [TD] and 19 with postural instability/gait difficulty [PIGD]) and 22 matched healthy controls. Whole-brain voxel-wise functional networks were constructed by measuring the temporal correlations of each pair of brain voxels. Functional connectivity strength was calculated to explore the brain network hubs. RESULTS: We found that both the TD and PIGD subtypes had comprehensive disrupted regions. These mainly involved the basal ganglia, cerebellum, superior temporal gyrus, pre- and postcentral gyri, inferior frontal gyrus, middle temporal gyrus, lingual gyrus, insula, and parahippocampal gyrus. Furthermore, the PIGD subgroup had more disrupted hubs in the cerebellum than the TD subgroup. These disruptions of hub connectivity were not correlated with the HY stage or disease duration. CONCLUSION: Our results emphasize the subtype-specific PD-related degeneration of brain hubs, providing novel insights into the pathophysiological mechanisms of connectivity dysfunction in different PD subgroups.


Assuntos
Mapeamento Encefálico/métodos , Encéfalo/fisiopatologia , Vias Neurais/fisiopatologia , Doença de Parkinson/fisiopatologia , Adulto , Idoso , Encéfalo/diagnóstico por imagem , Feminino , Humanos , Imagem por Ressonância Magnética/métodos , Masculino , Pessoa de Meia-Idade , Vias Neurais/diagnóstico por imagem , Doença de Parkinson/diagnóstico por imagem
7.
Arch Gynecol Obstet ; 295(2): 467-479, 2017 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-27975130

RESUMO

BACKGROUND: Epidemiological studies have provided controversial evidence of an association between alcohol intake and endometrial cancer (EC) risk. The World Cancer Research Fund/American Institute for Cancer Research classifies alcohol as having a "limited-no conclusion" grade of evidence in the Endometrial Cancer 2013 Report (the latest version). OBJECTIVE: The purpose of this meta-analysis is to systematically analyze the effect of alcohol intake on EC risk. METHODS: We conducted a dose-response meta-analysis of prospective cohort studies identified from the PubMed, Embase, Cochrane Library and China Biological Medicine databases. Categorical and dose-response meta-analyses were conducted to estimate the effects of alcohol on EC risk. RESULTS: A total of 10 studies involving 9766 cases and 1,612,798 participants were included in this meta-analysis. Overall, the relative risk(RR) for alcohol intake on EC was 1.04 (95% CI 0.88-1.22). The RRs for alcohol intake from wine, beer, and liquor were 1.10 (95% CI 0.80-1.51), 0.94 (95% CI 0.72-1.22), and 1.04 (95% CI 0.86-1.27), respectively). When alcohol consumption was stratified by drinking level, the RRs for moderate and heavy alcohol intake were 0.95 (95% CI 0.89-1.01) and 1.00 (95% CI 0.88-1.13), respectively. In the subgroup analyses, this association was not modified by other lifestyle factors or the characteristics of the study design and population. No significant associations were detected in the dose-response meta-analyses. CONCLUSIONS: Alcohol intake is not associated with EC regardless of the beverage choice and alcohol consumption level. More studies are warranted in other populations, such as Asians and Africans.


Assuntos
Consumo de Bebidas Alcoólicas/efeitos adversos , Neoplasias do Endométrio/etiologia , Feminino , Humanos , Estilo de Vida , Estudos Prospectivos , Risco
8.
Nat Prod Commun ; 9(7): 999-1001, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25230513

RESUMO

Alpinia zerumbet 'Variegata' is an aromatic medicinal plant, its foliage producing an intense, unique fragrant odor. This study identified 46 volatile compounds in the leaf tissue of this plant using headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS). The major compounds included 1, 8-cineole (43.5%), p-cymene (14.7%), humulene (5.5%), camphor (5.3%), linalool (4.7%), (E)-methyl cinnamate (3.8%), gamma-cadinene (3.3%), humulene oxide II (2.1%) and a-terpineol (1.5%). The majority of the volatiles were terpenoids of which oxygenated monoterpenes were the most abundant, accounting for 57.2% of the total volatiles. Alcohols made up the largest (52.8%) and aldehydes the smallest (0.2%) portions of the volatiles. Many bioactive compounds were present in the volatiles.


Assuntos
Alpinia/química , Cromatografia Gasosa-Espectrometria de Massas/métodos , Folhas de Planta/química , Microextração em Fase Sólida/métodos , Compostos Orgânicos Voláteis/química
9.
J Infect ; 69(1): 75-80, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-24631780

RESUMO

OBJECTIVES: To evaluate the correlation between IL-10 gene polymorphisms and hepatitis B infection. METHODS: Tag single nucleotide polymorphisms (SNPs) were used to investigate the relationship between IL-10 gene polymorphisms and susceptibility to chronic hepatitis B virus (HBV) infection by comparing 996 chronic HBV infection cases to 301 acute infection controls. RESULTS: This study found that rs3024490 G/T allele, located in the intron 1 region and highly prevalent in Chinese populations, was significantly different between the chronic HBV infection cases and the acute infection controls in single allele analysis, genetic models analysis, and haplotypes analysis. CONCLUSIONS: This suggested that the rs3024490 within IL-10 was associated with susceptibility to chronic hepatitis B in a Chinese Han population.


Assuntos
Predisposição Genética para Doença , Hepatite B Crônica/genética , Hepatite B Crônica/imunologia , Interleucina-10/genética , Polimorfismo de Nucleotídeo Único , Adulto , Grupo com Ancestrais do Continente Asiático , Humanos , Masculino
11.
Cancer ; 119(17): 3170-6, 2013 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-23765713

RESUMO

BACKGROUND: This study sought to compare the clinical outcomes of upper versus whole-neck prophylactic irradiation in the treatment of patients with node-negative nasopharyngeal carcinoma (NPC). METHODS: Between November 2005 and June 2012, 301 patients with node-negative NPC were randomly assigned to receive primary plus prophylactic upper neck irradiation (UNI, 153 patients) or primary plus whole-neck irradiation (WNI, 148 patients). Patients in both groups received irradiation to the primary tumor and the upper neck nodal regions, and patients in the WNI group also received irradiation to the lower neck. The main endpoint of the study was to compare the lower neck control rate between the 2 groups. RESULTS: With a median follow-up period of 39 months (range, 6-84 months), no patient in either group had a cervical node relapse. The overall survival at 3 years was 89.5% (95% confidence interval [CI] = 84.1%-95.0%) in the UNI group and 87.4% (95% CI = 81.4%-93.5%) in the WNI group (hazard ratio [HR] = 0.866, 95% CI = 0.41-1.82; P = .70). The 3-year relapse-free survival rate was 89.8% and 89.3% (95% CI = 84.2%-95.3% and 83.7%-94.8%, HR = 0.914, 95% CI = 0.42-2.00; P = .82), and the 3-year metastasis-free survival rate was 91.7% and 90.9% (95% CI = 87.0%-96.5% and 85.7%-96.1%) for the UNI and WNI groups, respectively (HR = 1.007, 95% CI = 0.44-2.32; P = .99). CONCLUSIONS: Prophylactic upper neck irradiation is sufficient for patients with node-negative NPC.


Assuntos
Linfonodos/efeitos da radiação , Neoplasias Nasofaríngeas/radioterapia , Prevenção Secundária/métodos , Adulto , Idoso , Carcinoma , Intervalo Livre de Doença , Feminino , Seguimentos , Humanos , Estimativa de Kaplan-Meier , Linfonodos/patologia , Masculino , Pessoa de Meia-Idade , Carcinoma Nasofaríngeo , Neoplasias Nasofaríngeas/patologia , Pescoço , Radioterapia/métodos , Radioterapia de Intensidade Modulada , Resultado do Tratamento
12.
PLoS One ; 7(12): e52073, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-23272213

RESUMO

Coxsackieviruses A10 (CV-A10) and A6 (CV-A6) have been associated with increasingly occurred sporadic hand-foot-mouth disease (HFMD) cases and outbreak events globally. However, our understanding of epidemiological and genetic characteristics of these new agents remains far from complete. This study was to explore the circulation of CV-A10 and CV-A6 in HFMD and their genetic characteristics in China. A hospital based surveillance was performed in three heavily inflicted regions with HFMD from March 2009 to August 2011. Feces samples were collected from children with clinical diagnosis of HFMD. The detection and genotyping of enteroviruses was performed by real-time PCR and sequencing of 5'UTR/VP1 regions. Phylogenetic analysis and selection pressure were performed based on the VP1 sequences. Logistic regression model was used to identify the effect of predominant enterovirus serotypes in causing severe HFMD. The results showed 92.0% of 1748 feces samples were detected positive for enterovirus, with the most frequently presented serotypes as EV-71 (944, 54.0%) and CV-A16 (451, 25.8%). CV-A10 and CV-A6 were detected as a sole pathogen in 82 (4.7%) and 44 (2.5%) cases, respectively. Infection with CV-A10 and EV-71 were independently associated with high risk of severe HFMD (OR = 2.66, 95% CI: 1.40-5.06; OR = 4.81, 95% CI: 3.07-7.53), when adjusted for age and sex. Phylogenetic analysis revealed that distinct geographic and temporal origins correlated with the gene clusters based on VP1 sequences. An overall ω value of the VP1 was 0.046 for CV-A10 and 0.047 for CV-A6, and no positively selected site was detected in VP1 of both CV-A10 and CV-A6, indicating that purifying selection shaped the evolution of CV-A10 and CV-A6. Our study demonstrates variety of enterovirus genotypes as viral pathogens in causing HFMD in China. CV-A10 and CV-A6 were co-circulating together with EV-71 and CV-A16 in recent years. CV-A10 infection might also be independently associated with severe HFMD.


Assuntos
Infecções por Coxsackievirus/epidemiologia , Enterovirus/genética , Doença de Mão, Pé e Boca/epidemiologia , China/epidemiologia , Surtos de Doenças , Enterovirus/classificação , Feminino , Genótipo , Doença de Mão, Pé e Boca/história , História do Século XXI , Humanos , Masculino , Dados de Sequência Molecular , Filogenia , Vigilância de Evento Sentinela , Sorotipagem , Proteínas Virais/genética
13.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 47(11): 680-3, 2012 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-23302431

RESUMO

OBJECTIVE: To investigate the effect of cytoskeleton reorganization inhibition with RNA interference on the activation of extracellular signal-regulated kinase (ERK1/2) in primary osteoblasts induced by fluid shear stress (FSS). METHODS: BALB/c mouse primary cultured osteoblasts were isolated by enzyme digestion technique. Osteoblasts were treated with LIM domain kinase 2 (LIM-2) specific siRNA or negative control siRNA, and then were loaded or unloaded by FSS of 1.2 Pa for 0, 5, 15, 30 and 60 min, respectively. The Western blotting was performed to detect the protein expression levels of P-ERK1/2 and ERK1/2, respectively. Two-way ANOVA and one-way ANOVA were used in data analysis. RESULTS: FSS loading for different time (0, 5, 15, 30, 60 min) treated with negative RNA inteference had significant effect on the levels of P-ERK/ERK ratio (0.047 ± 0.031, 0.253 ± 0.137, 0.390 ± 0.155, 0.613 ± 0.123, 0.680 ± 0.108, respectively, P < 0.01). Statistical analysis showed that there was significant interaction between FSS and cytoskeleton reorganization inhibition treated with RNA inteference on the levels of P-ERK/ERK ratio (P < 0.01). The levels of P-ERK/ERK ratio increased when osteoblasts were loaded for 5 - 15 min (0.623 ± 0.129 and 0.623 ± 0.064, respectively, P < 0.05) and returned to baseline at 30 min (0.333 ± 0.086), and then reached the peak at 60 min (0.667 ± 0.064, P < 0.01). CONCLUSIONS: FSS could activate ERK1/2 rapidly in primary cultured osteoblasts. Cytoskeleton reorganization inhibition treated with RNA interference speeded-up the activation of ERK1/2 by FSS, which could increase the sensitivity of ERK1/2 to FSS.


Assuntos
Citoesqueleto/metabolismo , Proteína Quinase 1 Ativada por Mitógeno/metabolismo , Proteína Quinase 3 Ativada por Mitógeno/metabolismo , Osteoblastos/enzimologia , Animais , Células Cultivadas , Citoesqueleto/fisiologia , Quinases Lim/genética , Quinases Lim/metabolismo , Mecanotransdução Celular , Camundongos , Camundongos Endogâmicos BALB C , Osteoblastos/citologia , Fosforilação , Interferência de RNA , RNA Interferente Pequeno , Estresse Mecânico
14.
Mol Diagn Ther ; 15(4): 211-9, 2011 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-21913743

RESUMO

OBJECTIVE: This study was designed to analyze expression patterns of estrogen receptor (ER), human epidermal growth factor receptor-2 (HER2/ERBB2), and nonmetastatic protein 23 (NM23-H1/NME1) proteins in patients with invasive ductal carcinoma and different menopausal status to identify their relationships with axillary lymph node metastasis. MATERIALS AND METHODS: 213 pre-menopausal and 177 post-menopausal women diagnosed with invasive ductal carcinoma were evaluated for ER, HER2, and NM23-H1 protein expression by immunohistochemistry. When HER2 immunoreactivity was equivocal (category 2+), specimens were confirmed by fluorescence in situ hybridization. RESULTS: ER expression showed no correlation with menopausal status or lymph node metastasis (each p > 0.05). However, expression of ER was associated with negative expression of HER2 (r = -0.214, p < 0.05) and positive expression of NM23-H1 (r = 0.137, p < 0.05) in the pre-menopausal group. Over-expression of HER2 was correlated with menopausal status (r = -0.107, p < 0.05) and lymph node metastasis in the ER-negative post-menopausal group (r = 0.222, p < 0.05). NM23-H1 was associated with less lymph node metastasis in the ER-positive pre-menopausal group (r = -0.237, p < 0.05). CONCLUSION: Our results indicated that expression patterns of ER, NM23-H1, and HER2 in primary breast cancer lesions warn that cells might have metastatic potential, which could assist clinicians to provide a more accurate prognosis and tailor therapeutic management for individual patients.


Assuntos
Neoplasias da Mama/metabolismo , Neoplasias da Mama/patologia , Carcinoma Ductal de Mama/metabolismo , Menopausa , Nucleosídeo NM23 Difosfato Quinases/metabolismo , Receptor ErbB-2/metabolismo , Receptores Estrogênicos/metabolismo , Adulto , Idoso , Biomarcadores Tumorais/genética , Biomarcadores Tumorais/metabolismo , Neoplasias da Mama/genética , Carcinoma Ductal de Mama/genética , Carcinoma Ductal de Mama/secundário , Feminino , Genes erbB-2 , Humanos , Imuno-Histoquímica , Hibridização in Situ Fluorescente , Metástase Linfática , Pessoa de Meia-Idade , Receptor ErbB-2/genética , Receptores Estrogênicos/genética
15.
Artigo em Inglês | MEDLINE | ID: mdl-20398793

RESUMO

The aim of this study was to investigate the cellular toxicity of copper-induced injury to the black tiger shrimp Penaeus monodon. The 24h, 48h, 72h and 96h LC(50) (median lethal concentration) of Cu(2+) on P. monodon (11.63+/-1.14g) were found to be 3.49, 1.54, 0.73 and 0.40mgL(-1), respectively. Total haemocyte count (THC), phagocytic activity, respiratory burst (RB), cytoplasmic free-Ca(2+) (cf-Ca(2+)) concentration and apoptotic cell ratio of shrimp were determined after exposure to different concentrations of Cu(2+) (0, 0.05, 0.5, 1.5 and 3.5mgL(-1)) for 0, 6, 12, 24 and 48h. There was no significant effect on the analytic indicator of shrimp exposed to 0.05mgL(-1) Cu(2+). THC decreased after Cu-exposure to 0.5mgL(-1) for 48h, 1.5mgL(-1) for 24h and 3.5mgL(-1) for 12h. Phagocytic activity decreased in P. monodon following 48h exposure to 3.5mgL(-1) Cu(2+). RB was induced after 6h exposure to 0.5, 1.5 and 3.5mgL(-1) Cu(2+). cf-Ca(2+) concentration increased after 48h exposure to 0.5mgL(-1) Cu(2+), and 12h exposure to 1.5 and 3.5mgL(-1) Cu(2+). The percentage of apoptotic cells increased to 9.5%, 16.3% and 18.6% respectively following 48h exposure to 0.5, 1.5 and 3.5mgL(-1) Cu(2+). These results indicate that Cu can induce oxidative stress, elevation of cf-Ca(2+) and cell apoptosis, and inhibit phagocytic activity in the shrimp P. monodon, and the lethal injury of Cu(2+) to P. monodon may be mainly due to the sharp reduction of THC caused by ROS-induced apoptosis.


Assuntos
Cálcio/metabolismo , Cobre/toxicidade , Hemócitos/efeitos dos fármacos , Penaeidae/efeitos dos fármacos , Poluentes Químicos da Água/toxicidade , Animais , Apoptose , Citoplasma/metabolismo , Hemócitos/metabolismo , Penaeidae/metabolismo , Fagocitose/efeitos dos fármacos , Explosão Respiratória/efeitos dos fármacos
16.
Acta Crystallogr Sect E Struct Rep Online ; 65(Pt 9): m1136, 2009 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-21577473

RESUMO

The title compound, [Cu(2)(C(14)H(13)N(2)O)(2)(N(3))(2)], was synthesized by the reaction of Cu(NO(3))(2)·3H(2)O with the Schiff base 2-[1-(2-pyridylmethyl-imino)eth-yl]phenol (HL) in methanol-water solution, adding NaN(3) as the bridging ligand. The asymmetric unit contains one half-mol-ecule, the other half being generated by the inversion center. Each Cu(II) atom shows a slightly distorted trigonal-pyramidal geometry formed by two N atoms and one O atom from one Schiff base ligand, by another O atom of a second Schiff base ligand and by an azide N atom. The crystal structure is stabilized by intermolecular C-H⋯N hydrogen bonds.

17.
Acta Crystallogr Sect E Struct Rep Online ; 65(Pt 8): m997, 2009 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-21583436

RESUMO

In the title compound, [Co(C(5)H(9)O(2))(2)(C(12)H(8)N(2))(H(2)O)], the Co(II) atom is coordinated in a distorted octahedral environment by three carboxyl O atoms of two trimethyl-acetate ligands, one aqua O atom and two N atoms from 1,10-phen-anthroline. The crystal structure is stabilized by O-H⋯O hydrogen bonds and π-π stacking inter-actions [inter-planar distance between inter-digitating 1,10-phenanthroline ligands = 3.378 (2) Å].

18.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 23(3): 316-9, 2006 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-16767673

RESUMO

OBJECTIVE: To analyze the association of that the polymorphisms and haplotypes of Taq I site in beta fibrinogen gene and the single nucleotide sites -455 G/A, -249 C/T, -148 C/T, +1689T/G, Bsm A I G/C, 448 G/A, Bcl I G/A, Hinf I A/C in beta-fibrinogen gene are linked up with the ischemic stroke(IS). METHODS: Turbidmetric assay was used to measure the plasma fibrinogen level of one hundred and sixty cases with ischemic stroke and one hundred and thirty healthy individuals from Hainanese Han population. The polymorphisms and genotypes were characterized by PCR-RFLP. Hardy-Weinberg equilibrium and statistical differences of allelic, genotype and haplotype frequencies were obtained by Chi-square test. Pairwise linkage disequilibrium was calculated and haplotypes of nine or four polymorphisms were estimated by the EH + program. RESULTS: There were highly significant differences in genotype frequencies and allelic frequencies of the polymorphisms -455 G/A, -148 C/T, 448 G/A, which happened between the IS group and control subjects (P< 0.01). However, the significant differences of the allelic frequencies in the other six polymorphisms were not found between the IS group and the control (P> 0.05). The odds ratio(OR) with the rare alleles of A -455, T -148 and A 448 is 2.46, 2.30 and 2.08 (95% confidence interval 1.153%-3.924%, 1.429%-3.694% and 1.298%-3.329%) respectively. No definite haplotype block was found by linkage disequilibrium analysis in the control group and the IS group. Association of haplotypes constructed from the nine polymorphisms with IS was not found. Among the haplotypes constructed from four polymorphisms including -455 G/A, -148 C/T, 448 G/A alleles, haplotype differences were found between the control group and the IS group. Haplotypes with G -455, C -148, G448 alleles appeared more frequently in control group(P< or = 0.01), whereas haplotypes with A -455, T -148, A 448 occurred more frequently in the IS group(P< 0.01). CONCLUSION: The results of multi-allele and haplotype analysis indicated that the polymorphisms -455 G/A, -148 C/T, 448 G/A in beta fibrinogen gene were the possible risk factors associated with the occurrence of ischemic stroke in Hainan Han population.


Assuntos
Fibrinogênio/genética , Haplótipos/genética , Polimorfismo de Nucleotídeo Único/genética , Acidente Vascular Cerebral/genética , Alelos , Isquemia Encefálica/complicações , Feminino , Frequência do Gene , Predisposição Genética para Doença/genética , Genótipo , Humanos , Desequilíbrio de Ligação/genética , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , Acidente Vascular Cerebral/etiologia
19.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 22(4): 457-61, 2005 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-16086292

RESUMO

OBJECTIVE: To investigate the allelic frequencies of polymorphisms of alpha Taq I and beta Bcl I, Hinf I A/C, 448 G/A, beta BsmA I G/C, +1689T/G, -148C/T, -249C/T, -455G/A in Hainan Han population and their association with plasma fibrinogen level. METHODS: Turbidmetric assay was used to measure plasma fibrinogen level of two hundred and thirty-eight healthy individuals. The genotypes were characterized by PCR-RFLP and sequence analysis. The relationships between the genotypes and plasma fibrinogen levels were analyzed by t test and ANOVA. RESULTS: The frequencies of the rare alleles of alpha Taq I and beta Bcl I, Hinf I A/C, 448 G/A, beta BsmA I G/C, +1689T/G, -148C/T, -249C/T, -455G/A polymorphisms were 0.445, 0.239, 0.134, 0.235, 0.273, 0.241, 0.265, 0.441, 0.254 respectively. In the general population, the plasma fibrinogen level is significantly higher in the groups of genotypes -455GA and AA, -148CT and TT, alpha Taq I T1T1 than in the group of wild types(P=0.004, 0.015 and 0.043 respectively). In the men, plasma fibrinogen level is significantly higher in the groups of genotypes -455GA and AA, -148CT and TT, alpha Taq I T1T1, alpha Taq I T1T2 than in the group of wild types(P=0.001, 0.023, 0.003 and 0.032 respectively). In the women, no significant genotype association with plasma fibrinogen level was detected. CONCLUSION: There was linkage disequilibrium between the fibrinogen gene loci. The beta -455G/A beta 448G/A, alpha Fg Taq I polymorphisms were associated with the difference in plasma fibrinogen in men. A(-455), T(-148) and alpha Taq I T1 alleles were associated with higher fibrinogen levels.


Assuntos
Fibrinogênio/genética , Polimorfismo Genético , Adulto , China , Feminino , Fibrinogênio/metabolismo , Frequência do Gene , Genética Populacional , Genótipo , Humanos , Desequilíbrio de Ligação , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição
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