Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 7 de 7
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Bioresour Technol ; 296: 122319, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31689612

RESUMO

A kind of reduced graphene oxide decorated with titanium-based (RGO/TiO2) composites are successfully synthesized and employed in this current study as a novel nonprecious metal catalyst for enhancing bioelectricity generation and cathodic oxygen reduction reaction (ORR) in single chamber microbial fuel cells (MFCs). Compared with commercial Pt/C, RGO/TiO2 shows obviously enhanced oxygen reduction reaction activity due to the appropriately-permeated, large electrochemical active area, enough exposure of electrocatalytic active sites of RGO/TiO2. The air-cathode MFC with RGO/TiO2-1 cathode achieves 1786.7 mW m-3 of power density, 86.7% ±â€¯1.2% of COD removal and 31.6% ±â€¯1.1% of CE, which are higher than commercial Pt/C. Moreover, RGO/TiO2-1 cathode exhibits high-effective electrocatalytic activity, and the power density of RGO/TiO2-1 can keep a stable level and only has a minor decline (5.35%) during 30-cycles operation. These results indicate that RGO/TiO2-1 is a potential cathode catalyst, markedly enhancing cathode ORR, wastewater treatment efficiency, and bioelectricity generation of MFC.


Assuntos
Oxigênio , Titânio , Eletrodos , Características da Família , Grafite
2.
Clin Chim Acta ; 495: 507-511, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31152696

RESUMO

BACKGROUND: The pneumatic tube system (PTS) is widely established in clinical laboratories. We aimed to evaluate the impacts of PTS on high-sensitivity cardiac troponin T (hs-cTnT) assays. METHODS: The hemolysis distribution of hs-cTnT PTS specimens from emergency department (ED) were determined by hemolysis index (HI). Grouped samples from 15 healthy volunteers were delivered to the laboratory via manual delivery (MD) or PTS. Interference studies were conducted to access the influence of different hemolysis degrees on hs-cTnT assays. RESULTS: 7.26% PTS specimens from ED were hemolyzed in clinic. Compared with MD samples, we found highly elevated free plasma hemoglobin (Hb) in PTS samples. Hs-cTnT was interfered negatively with free Hb (R = -0.625, P < .001), and it was also validated in interference studies (R ≥ -0.820, all P ≤ .001). Clinically significant bias occurred in each hs-cTnT concentration at 100 mg/dl free Hb (Bias≥ - 13.85%, all P < .05). Moreover, bias of hs-cTnT assays at 50 mg/dl free Hb was approaching 10%, especially at 30 ng/l hs-cTnT concentration (Bias: -11.72%, P < .001). CONCLUSIONS: PTS could increase the frequency of specimen hemolysis which might cause false decrease in hs-cTnT assays. Hence, clinicians should be aware of the increased measurement bias in hs-cTnT from hemolyzed PTS samples with free Hb ≥50 mg/dl.


Assuntos
Análise Química do Sangue/instrumentação , Limite de Detecção , Miocárdio/metabolismo , Troponina T/sangue , Reações Falso-Negativas , Feminino , Voluntários Saudáveis , Humanos , Masculino , Troponina T/metabolismo , Adulto Jovem
3.
Oncotarget ; 8(49): 85670-85679, 2017 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-29156749

RESUMO

Bone metastases often occur in prostate cancers, lung cancers and breast cancers. Bone alkaline phosphatase (BALP) is one of the most commonly used serological markers for clinical evaluation of bone metabolism. Here, we reported that high concentrations of uric acid (UA) caused decrease of BALP levels and revealed that the effect of high concentrations of UA on the BALP expression was through inhibition of its promoter activity. Our results suggested physicians to think about serum UA status of patients with advanced cancer to avoid misdiagnosis when BALP was used to diagnose or assess the extent of bone metastases.

4.
Int J Clin Exp Med ; 8(6): 9758-64, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26309653

RESUMO

PURPOSE: (18)F-FLT-PET imaging was proposed as a tool for measuring in vivo tumor cell proliferation and detecting sub-volumes to propose escalation in radiotherapy. The aim of this study was to validate whether high FLT uptake areas in (18)F-FLT PET/CT are coincident with tumor cell proliferation distribution indicated by Ki-67 staining in non-small cell lung cancer, thus provide theoretical support for the application of dose painting guided by (18)F-FLT PET/CT. MATERIALS AND METHODS: Twelve treatment naive patients with biopsy proven NSCLC underwent (18)F-FLT PET/CT scans followed by lobectomy were enrolled. The surgical specimen was dissected into 4-7 µm sections at approximately 4-mm intervals. The best slice was sort out to complete Ki-67 staining. Maximum Ki-67 labelling Index and SUVmax of the corresponding PET image was calculated. The correlation between Ki-67 Labelling Index and SUVmax of FLT was determined using Spearman Correlation analysis. High uptake areas and high proliferating areas were delineated on the two images, respectively, and their location was compared. RESULTS: The maximal SUV was 3.26 ± 0.97 (1.96-5.05), maximal Ki-67 labeling index was 49% ± 27.56% (5%-90%). Statistical analysis didn't reveal a significant correlation between them (r = -0.157, P = 0.627, > 0.05). 9 patients can contour high proliferating area on Ki-67 staining slice, and eight can contour the high uptake areas. In 4 patients, we can observe a generally close distribution of high uptake areas and high proliferating areas, in one patient, both the uptake level and proliferation status was low, while the others didn't not find a significant co-localization. CONCLUSION: Noninvasive (18)F-FLT PET assessing the proliferative status may be a valuable aid to guide dose painting in NSCLC, but it needs to be confirmed further.

5.
Dalton Trans ; 43(8): 3137-43, 2014 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-24316886

RESUMO

A SnO/SnO2/GNS composite with controlled oxidation states and composition has been prepared through simple one-pot reduction of an EG suspension of SnCl2 and graphene oxide. The as-prepared composite was characterized by XRD, FT-IR, XPS, SEM, TEM and BET. SnO and SnO2 nanoparticles are uniformly distributed on the surface of the graphene. Taking advantage of the high electron conductivity of graphene and the large theoretical capacity of SnO, this SnO/SnO2/GNS composite exhibits high charge/discharge capacity, good cycling stability and good rate capability. A specific discharge capacity of approximately 464.2 mA h g(-1) is retained after being charged/discharged at a current density of 1000 mA g(-1) for 30 cycles.

6.
Nan Fang Yi Ke Da Xue Xue Bao ; 29(5): 880-3, 2009 May.
Artigo em Chinês | MEDLINE | ID: mdl-19460698

RESUMO

OBJECTIVE: To obtain monoclonal antibodies (mAb) against LI-cadherin and investigate their effects on the proliferation of human hepatocellular carcinoma cells. METHODS: Balb/c mice were immunized with recombinant LI-cadherin, and hybridoma cell lines secreting monoclonal antibodies against LI-cadherin were established with routine cell fusion and subcloning approach. The specificity of these mAbs was determined by enzyme-linked immunosorbent assay (ELISA) and Western blotting. The effect of the mAbs obtained on the growth of HepG2 cells was assessed using inverted microscope and MTT assay. RESULTS: Two hybridoma cell lines (F001 and F002) stably secreting specific mAbs were obtained. Western blot analysis showed that the two antibodies specifically recognized LI-cadherin antigen derived from human eucaryotic cells or tissue. Treatment of the HepG2 cells with the mAbs resulted in reduced viable cell number and changes in the cell morphologies, and the two mAbs inhibited the proliferation of HepG2 cells in a concentration-dependent manner (P<0.05). CONCLUSION: The two specific mAbs obtained can inhibit the proliferation of HepG2 cells in vitro, which facilitates further study of the relationship between LI-cadherin and tumors.


Assuntos
Anticorpos Monoclonais/biossíntese , Anticorpos Monoclonais/farmacologia , Caderinas/imunologia , Proliferação de Células/efeitos dos fármacos , Neoplasias Hepáticas/patologia , Animais , Anticorpos Monoclonais/imunologia , Caderinas/farmacologia , Carcinoma Hepatocelular/patologia , Linhagem Celular Tumoral , Feminino , Humanos , Hibridomas/metabolismo , Camundongos , Camundongos Endogâmicos BALB C
7.
J Zhejiang Univ Sci B ; 9(10): 818-22, 2008 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-18837110

RESUMO

A new stem rot disease is found to occur naturally on Arabidopsis plants in greenhouses of Fuzhou, China. In order to identify its pathogen, we conducted a series of fungal isolation and purification, plant reinoculation, and ascus and ascospore induction from the sclerotia. The isolate caused typical water-soaked lesions after reinoculation and produced sclerotia both on Arabidopsis plants and culture medium plates, and the sclerotia could be induced to produce discal apothecia and 8 binucleate ascospores per ascus. These disease symptom and fungal morphology data revealed that the fungus Sclerotinia sclerotiorum (Lib.) de Bary was the pathogen for Arabidopsis stem rot. To confirm this, we further amplified its large subunit ribosomal DNA (LSU rDNA) by polymerase chain reaction (PCR), and compared the sequence with the known LSU rDNA sequences in GenBank. The results show that the sequence shares the highest identities with the LSU rDNAs of different S. sclerotiorum strains. Taking all these data together, we concluded that the fungus that caused the Arabidopsis stem rot is S. sclerotiorum (Lib.) de Bary. This is the first report that Arabidopsis is naturally infected by S. sclerotiorum.


Assuntos
Ascomicetos/isolamento & purificação , Ascomicetos/patogenicidade , Doenças das Plantas/microbiologia , Arabidopsis/microbiologia , Ascomicetos/classificação , Ascomicetos/genética , Sequência de Bases , China , DNA Fúngico/genética , DNA Ribossômico/genética , Filogenia , Caules de Planta/microbiologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA