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1.
Obes Surg ; 2019 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-31758470

RESUMO

BACKGROUND: Obesity has become a global epidemic. Surgical treatment of obesity and metabolic disorders in China is increasing rapidly, but it is still a new discipline even to health professionals. As an important member of the multidisciplinary team, the knowledge and attitudes of nurses provide crucial health care to the patients and support to surgeons. OBJECTIVES: To study the Chinese nurses' knowledge of obesity and metabolic disorders, and attitudes towards bariatric surgery and to improve their capability of work in this new discipline. METHODS: This is a multicenter study, with the questionnaire distributed to cooperative hospitals in the form of an electronic questionnaire by the First Affiliated Hospital of Jinan University in April 2018. A questionnaire was designed to investigate nurses' demographic, knowledge, and attitude towards obesity, weight loss, and bariatric surgery. RESULTS: A total of 5311 questionnaires were received, with an effective rate of 91.8% (4878 questionnaires); 65.2% of nurses had a normal BMI. Nurses generally had a high knowledge of obesity and related cardiovascular diseases (98.6%) and type 2 diabetes mellitus (90.2%). However, there was a lack of knowledge in other related aspects, for example its relations to carcinoma (49.5%), gastroesophageal reflux disease (40.1%), and psychological disorders (49.1%), which are controversial issues in bariatric surgery. It was found that education (p < 0.05) had an important influence to nurses' knowledge about the comorbidities of obesity. Female nurses had a higher tendency to choose weight loss than males, but male nurses did physical exercise more frequently than females (p < 0.05). Their acceptance of safety (25.1%) and efficacy (22.9%) of bariatric surgery is low, with concerns predominantly about postoperative complications and adverse effects. Surgical nurses had a more optimistic attitude towards surgery (p < 0.05). CONCLUSIONS: Chinese nurses have poor knowledge of obesity-related metabolic disorders and also have poor acceptance of surgical treatment modalities. Our findings suggest that it is crucial to enhance the continuing education of Chinese nurses for obesity, metabolic disorders, and bariatric surgery.

2.
J Hematol Oncol ; 12(1): 117, 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31747962

RESUMO

The original article [1] contains an error in authorship whereby author, Robert Weinkove's name is mistakenly inverted. The configuration noted in this Correction article should be considered instead along with author's updated affiliation.

3.
Dis Markers ; 2019: 2424751, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31485275

RESUMO

Background: Renal fibrosis is a common outcome of all pathological types of chronic kidney disease (CKD). However, the noninvasive detection of renal fibrosis remains a challenge. Methods: We collected urine samples from 154 biopsy-proven IgA nephropathy (IgAN) patients and 61 healthy controls. The expression of mTOR was measured and the correlation with renal function parameter and pathological indicators. The receiver operating characteristic (ROC) curve for the diagnosis of IgAN and renal fibrosis was calculated. Results: The urinary mammalian target of rapamycin (mTOR) expression was decreased in IgAN patients. The expression of mTOR was correlated with serum creatinine, blood urea nitrogen, estimated glomerular filtration rate, 24 h proteinuria, and cystatin C. Further, the urinary mTOR expression was significantly decreased in severe renal fibrosis patients compared with mild or moderate renal fibrosis patients. Urinary mTOR expression was correlated with score of tubulointerstitial fibrosis (TIF) and score of glomerular sclerosis. The ROC curve showed that mTOR can diagnose IgAN at a cut-off value of 0.930 with the sensitivity of 90.2% and specificity of 73.8% and renal fibrosis at a cut-off value of 0.301 with the sensitivity of 71.7% and specificity of 64.8%. Conclusion: Urinary mTOR mRNA expression was a potential biomarker for diagnosis of IgAN and renal fibrosis in IgAN patients.

4.
J Sep Sci ; 42(19): 3095-3101, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31328867

RESUMO

Phthalate esters are easily released from plastics materials and migrate into the soil and water environment, causing serious pollution and posing a great threat to the health of human beings. A novel temperature-sensitive extractant combined with liquid-liquid microextraction was developed to preconcentrate three phthalates in the water environment. To optimize the extraction efficiency for the three phthalate esters, various parameters, including polymer molecular weight, salt type, salt addition, adsorption time, desorption solvent, desorption volume, and desorption time have been studied. Under optimal conditions, limits of detection and limits of quantification were in the range of 0.007-0.120 and 0.021-0.350 µg/L, respectively. Linearities varied in the range of 5-1000 µg/L, with the correlation coefficients of 0.9867-0.9997. The preconcentration factors were in the range of 25-75. The relative recoveries of the three phthalate esters were in the range of 82.2-105.6% at the spiked levels. The relative standard deviations were in the range of 0.7-9.2% based on triplicate measurements. The results indicate that the temperature-sensitive material is a good extractant for phthalate esters in water samples.

5.
Spectrochim Acta A Mol Biomol Spectrosc ; 221: 117211, 2019 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-31158765

RESUMO

Doping graphene quantum dots (GQDs) with heteroatoms can change their band gap and electronic density, thus enhancing their fluorescence quantum yield (QY). In this work, we for the first time reported a nontoxic, rapid, and one-pot hydrothermal method to synthesize sulfur and phosphorus co-doped GQDs (S, P-GQDs). Citric acid was functioned as a carbon source, whereas sodium phytate and anhydrous sodium sulfate are used as the P and S sources, respectively, in this bottom-up synthesis. The resulting S, P-GQDs exhibit high heteroatomic doping ratios of 9.66 at.% for S and 3.34 at.% for P, and higher QY than those obtained from monoatomic doped GQDs. Additionally, the as-prepared S, P-GQDs exhibit excitation-dependent behavior, pH sensitivity between 8.0 and 13.0, high tolerance of ionic strength. More importantly, the as-synthesized S, P-GQDs show a sensitive and selective behavior for sensing nitrite (NO2-) in the concentration range of 0.7-9 µmol/L, and the detection limit was as low as 0.3 µmol/L. Additionally, the S, P-GQDs was successfully used in detecting NO2- in pickled foods, showing their promise for potential applications in realistic analysis.

6.
Clin Transl Sci ; 12(6): 564-572, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31169344

RESUMO

In this paper, a special case for bioequivalence evaluation of oral formulations is discussed. Drug formulations with different forms of active moieties (e.g., free base and salt) may yield different dissolution characteristics and, thus, differ in absorption at elevated gastric pH. However, routine bioequivalence trials using subjects with normal gastric pH (i.e., ~ 1) may fail to identify these differences because dissolution/absorption profiles of the two formulations at normal gastric pH are similar. In the case of palbociclib, it is confirmedthat the free base and salt formulations showed different absorption in patients with different gastric pH. Significant reduction in drug absorption was observed only in patients with elevated gastric pH using free base formulation. The discovery that the free base had significantly reduced absorption hinged on the inclusion of enough patients with elevated gastric pH to detect a difference in a bioequivalence trial. This raises a concern, as demonstrated through simulation, that dissolution/absorption differences in other formulations could be missed in routine bioequivalence trials. Aside from differences in active pharmaceutical ingredients (APIs), other factors, such as changes in excipients or manufacturing methods, may also lead to exposure differences between formulations at elevated gastric pH. For formulations containing different forms of the same active moiety or the same API and showing different dissolution profiles at elevated pH (i.e., pH ~ 4-6.8), evaluation of bioequivalence with gastric pH modulators (e.g., a H2 blocker) in addition to routine bioequivalence assessments may help to ensure therapeutic equivalence in patients with elevated gastric pH.

7.
Int Immunopharmacol ; 74: 105663, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31200338

RESUMO

Extracellular vesicles (EVs) secreted by mesenchymal stem cells (MSC-EVs) are taken more seriously as immunomodulatory and anti-inflammatory agents. We studied the therapeutic effects of MSC-EVs on allergic contact dermatitis (ACD), a typical T cell-mediated disorder. A contact hypersensitivity (CHS) mouse model for ACD was established and treated by intravenous MSC-EVs injection. We found that human umbilical cord MSC-EVs could significantly prevent the pathology of CHS, including reduced ear swelling and leukocyte infiltration. Injection of MSC-EVs significantly inhibited CD8+IFN-γ+ cytotoxic T (Tc1) cells and CD4+IFN-γ+ type 1 helper T (Th1) cells, and reduced the level of pro-inflammatory Tumor Necrosis Factor-alpha (TNF-α) and interferon gamma (IFN-γ), and induced CD4+CD25+Foxp3+ regulatory T cells (Tregs) and the level of anti-inflammatory IL-10. In vitro, MSC-EVs also suppressed Tc1 and Th1 cells and induced Tregs and the related cytokines, further indicating the immune regulatory role of MSC-EVs. Interestingly, PKH26-labeled MSC-EVs were found to be directly internalized by CD3+ T cells, resulting in reduced signal transducer and activator of transcription 1 (STAT1) protein levels in vitro. In summary, MSC-EVs can prevent the onset of CHS by inhibiting Tc1 and Th1 immune responses and inducing the Tregs phenotype in vivo and in vitro. The mechanism by which MSC-EVs influence CD3+ T cells might partially involve targeting STAT1 in vitro. Therefore, MSC-EVs are ideal candidates for cell-free immunomodulatory therapy for T cell-mediated diseases such as ACD.

8.
Mikrochim Acta ; 186(5): 322, 2019 05 02.
Artigo em Inglês | MEDLINE | ID: mdl-31049692

RESUMO

A specific and sensitive colorimetric aptasensor is described for the determination of Malachite Green (MG). It is exploiting the inhibition of the peroxidase-like activity of gold nanoparticles (AuNPs). The AuNPs act as enzyme mimics that catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by H2O2 to yield a dark blue solution. The catalytic activity is inhibited by hexadecyl trimethyl ammonium ion, specifically by cetyltrimethylammonium bromide (CTAB), which causes the aggregation of AuNPs. If a (negatively charged) RNA-aptamer against MG is added, it binds to the positively charged CTAB and prevents aggregation. This enhances the enzyme mimicking activity of the AuNPs and leads to the formation of a dark blue solution. However, in the presence of MG, the aptamer binds to MG, and leads to the aggregation of AuNPs again. The aggregated AuNPs possess a light blue color. A colorimetric method (best performed at 650 nm) was work out that can detect MG in a concentration range from 10 to 500 nmol L-1. The detection limit based on 3σ/k criterion is 1.8 nmol L-1. The assay is highly specific and accurate. Recoveries from spiked real samples (aquaculture water) ranged from 80% to 120%. Graphical abstract Based on the inhibition of cetyltrimethyal ammonium ion and the enhancement of RNA-aptamer, the differences of the peroxidase-like activities of AuNPs can be greatly enlarged with and without MG, by which a colorimetric aptasensor can be constructed for the detection of Malachite Green (MG).

9.
J Mol Histol ; 50(4): 343-354, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31111288

RESUMO

Cardiac hypertrophy is a compensatory response in reaction to mechanical load that reduces wall stress by increasing wall thickness. Chronic hypertrophic remodeling involves cardiac dysfunction that will lead to heart failure and ultimately death. Studies have been carried out on cardiac hypertrophy for years, whereas the mechanisms have not been well defined. Tamarixetin (TAM), a natural flavonoid derivative of quercetin, have been demonstrated possessing anti-oxidative and anti-inflammatory effects on multiple diseases. However, little is known about the function of TAM on the development of cardiac hypertrophy. Here, we found TAM could alleviate pressure-overload-induced cardiac hypertrophy in transverse aortic constriction (TAC) mouse model, assessed by ventricular weight/body weight, lung weight/body weight, echocardiographic parameters, as well as myocyte cross-sectional area and the expression of ANP, BNP and Myh7. In vitro, TAM showed a dose dependent inhibitory effect on phenylephrine-induced hypertrophy in H9c2 cardiomyocytes. Furthermore, TAM reversed cardiac remodeling of stress overloaded heart by suppressing apoptosis and the expression of fibrotic-related genes, reduced oxidative stress and ROS production both in vivo and in vitro. In addition, TAM could negatively modulate TAC-induced nuclear translocation of NFAT and the activation of PI3K/AKT signaling pathways. Therefore, these data indicate for the first time that TAM has a protective effect on experimental cardiac hypertrophy and might be a novel candidate for the treatment of cardiac hypertrophy in clinic.

10.
J Cell Biochem ; 120(9): 14813-14821, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31119774

RESUMO

Meibomian gland dysfunction (MGD) is an epidemic chronic ocular inflammation. However, little is known about its effective treatment. Here, this study identified important MGD-related genes, core regulators, and potential drugs and their targets though integrating a series of bioinformational analyses. First, there were 665 differentially expression genes (DEGs) were identified. Then, 56 coexpression modules were exacted based on the expression of DEGs and their interactors. Moreover, core transcription factors (TF) significantly regulated modules were identified, including RELA, HIF1A, SIRT1, and MYC, which related to variety of eye diseases. Finally, the prediction of potential drugs and the identification of their target were performed. The results showed that artenimol, copper, and glutathione may have the remarkable curative effect or the toxicology to MGD. Moreover, their targets module gene LDHA (lactate dehydrogenase A), ENO1 (enolase 1), ALB (albumin), and PKM (pyruvate kinase M) are play important role in eye diseases. It suggests that these potential drugs may be useful for the treatment of MGD by acting on their targets. It provides valuable references for drug redirection and new drug development for drug developers, and provides individualized treatment strategies for tarsal gland dysfunction.

11.
Gene ; 705: 5-15, 2019 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-30999026

RESUMO

BACKGROUND: In recent years, the molecular mechanism of plant growth and development has been reported in detail. GRAS genes, a plant-specific family of transcription factor, play critical roles in the process. GRAS transcription factors are associated with axillary shoot meristem formation, radial root patterning, phytohormones (gibberellins) signal transduction, light signaling, and abiotic or biotic stress. OBJECTIVE: Here, we firstly investigated GRAS gene family in Dendrobium catenatum, an important medicinal and flowering orchid in China. METHODS: The GRAS gene family in D. catenatum was cloned based on RNA-Seq data. Selected GRAS genes were introduced into Escherichia coli to express proteins. RESULTS: Based on phylogenetic relationship with the Arabidopsis and Oryza GRAS family members, 47 GRAS genes from D. catenatum are identified and their deduced proteins are classified into 11 subgroups. Most of these GRAS genes contain one exon and closely related members in the phylogenetic tree have similar motif composition. Our result also reveals that GRAS genes in D. catenatum are widely distributed and expressed in different tissue. In addition, 35 GRAS genes are successfully cloned from different subgroups and 7 DoGRAS fusion proteins are induced using E. coli system. Moreover, 8 genes were up-regulated in different tissue following exposure to heat and salt stresses. CONCLUSION: Our findings provide valuable information and candidate genes for future functional analysis for improving the resistance of D. catenatum growth.


Assuntos
Clonagem Molecular/métodos , Dendrobium/genética , Análise de Sequência de RNA/métodos , Fatores de Transcrição/genética , Dendrobium/metabolismo , Escherichia coli/genética , Evolução Molecular , Regulação da Expressão Gênica de Plantas , Família Multigênica , Filogenia , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Estresse Fisiológico , Distribuição Tecidual , Fatores de Transcrição/metabolismo
12.
Nano Lett ; 19(5): 2993-3001, 2019 05 08.
Artigo em Inglês | MEDLINE | ID: mdl-30964695

RESUMO

Currently, various oncolytic adenoviruses (OA) are being explored in both preclinical and clinical virotherapy. However, the pre-existing neutralizing antibodies (nAbs) and poor targeting delivery are major obstacles for systemically administered OA. Therefore, we designed bioengineered cell membrane nanovesicles (BCMNs) that harbor targeting ligands to achieve robust antiviral immune shielding and targeting capabilities for oncolytic virotherapy. We employed two distinct biomimetic synthetic approaches: the first is based on in vitro genetic membrane engineering to embed targeting ligands on the cell membrane, and the second is based on in vivo expression of CRISPR-engineered targeting ligands on red-blood-cell membranes. The results indicate that both bioengineering approaches preserve the infectivity and replication capacity of OA in the presence of nAbs, in vitro and in vivo. Notably, OA@BCMNs demonstrated a significant suppression of the induced innate and adaptive immune responses against OA. Enhanced targeting delivery, viral oncolysis, and survival benefits in multiple xenograft models were observed without overt toxicity. These findings reveal that OA@BCMNs may provide a clinical basis for improving oncolytic virotherapy by overcoming undesired antiviral immunity and enhancing cancer cell selectivity via biomimetic synthesis approaches.


Assuntos
Anticorpos Neutralizantes/farmacologia , Sistemas de Liberação de Medicamentos , Terapia Viral Oncolítica , Adenoviridae/química , Adenoviridae/genética , Animais , Anticorpos Neutralizantes/química , Linhagem Celular Tumoral , Membrana Celular/química , Membrana Celular/genética , Engenharia Genética , Humanos , Camundongos , Vírus Oncolíticos/genética , Vírus Oncolíticos/imunologia , Ensaios Antitumorais Modelo de Xenoenxerto
13.
J Clin Endocrinol Metab ; 104(11): 5043-5052, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-30994896

RESUMO

CONTEXT: Maternal thyroid hormones during pregnancy play a critical role in fetal development. However, whether maternal heavy metal exposure affects their thyroid hormones and the effects on fetal growth are still unclear. OBJECTIVE: To explore the effect of heavy metal exposure on maternal thyroid hormones and the potential mediation role of thyroid hormones on birth outcomes. METHODS: Concentrations of heavy metals in urine samples and thyroid hormones in blood samples of 675 pregnant women were measured during early pregnancy in a cohort study conducted in China. Multivariable linear regressions were applied to explore the associations of maternal urinary heavy metal levels with both maternal thyroid hormones and birth outcomes. Mediation analyses were performed to assess the mediation role of thyroid hormones in these associations. RESULTS: Maternal urinary vanadium (V) exhibited an inverse association with free T3 (FT3) and FT3/free T4 (FT4) ratio levels. Urinary arsenic (As) and lead (Pb) had inverse relationships with FT3. We also observed the positive associations of maternal FT3 and FT3/FT4 ratio with birthweight. The mediation analyses suggested that 5.33% to 30.57% of the associations among V, As, and Pb levels and birth size might be mediated by maternal FT3 or FT3/FT4 ratio. CONCLUSIONS: We have shown that maternal exposures to V, As, and Pb at early pregnancy were associated with decreased maternal FT3 or FT3/FT4 ratio, which might contribute to reduced birthweight. Mediation analyses indicated that maternal thyroid hormone was a possible mediator of the association between urinary heavy metals and birth size.

14.
Nat Commun ; 10(1): 1303, 2019 03 21.
Artigo em Inglês | MEDLINE | ID: mdl-30899015

RESUMO

In plants, flowering time is controlled by environmental signals such as day-length and temperature, which regulate the floral pathway integrators, including FLOWERING LOCUS T (FT), by genetic and epigenetic mechanisms. Here, we identify an H3K27me3 demethylase, JUMONJI 13 (JMJ13), which regulates flowering time in Arabidopsis. Structural characterization of the JMJ13 catalytic domain in complex with its substrate peptide reveals that H3K27me3 is specifically recognized through hydrogen bonding and hydrophobic interactions. Under short-day conditions, the jmj13 mutant flowers early and has increased FT expression at high temperatures, but not at low temperatures. In contrast, jmj13 flowers early in long-day conditions regardless of temperature. Long-day condition and higher temperature induce the expression of JMJ13 and increase accumulation of JMJ13. Together, our data suggest that the H3K27me3 demethylase JMJ13 acts as a temperature- and photoperiod-dependent flowering repressor.


Assuntos
Proteínas de Arabidopsis/química , Arabidopsis/genética , Flores/genética , Regulação da Expressão Gênica de Plantas , Histona Desmetilases com o Domínio Jumonji/química , Agrobacterium tumefaciens/genética , Agrobacterium tumefaciens/metabolismo , Sequência de Aminoácidos , Arabidopsis/crescimento & desenvolvimento , Arabidopsis/metabolismo , Arabidopsis/efeitos da radiação , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Sítios de Ligação , Clonagem Molecular , Cristalografia por Raios X , Flores/crescimento & desenvolvimento , Flores/metabolismo , Flores/efeitos da radiação , Expressão Gênica , Regulação da Expressão Gênica no Desenvolvimento , Histonas/química , Histonas/genética , Histonas/metabolismo , Ligações de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Histona Desmetilases com o Domínio Jumonji/genética , Histona Desmetilases com o Domínio Jumonji/metabolismo , Luz , Modelos Moleculares , Mutação , Peptídeos/química , Peptídeos/genética , Peptídeos/metabolismo , Fotoperíodo , Ligação Proteica , Conformação Proteica em alfa-Hélice , Conformação Proteica em Folha beta , Domínios e Motivos de Interação entre Proteínas , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Especificidade por Substrato , Temperatura Ambiente , Tabaco/genética , Tabaco/metabolismo
15.
Adv Mater ; 31(16): e1808024, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30848541

RESUMO

X-ray-induced photodynamic therapy (X-PDT) combines both the advantages of radiotherapy (RT) and PDT, and has considerable potential applications in clinical deep-penetrating cancer therapy. However, it is still a major challenge to prepare monodisperse nanoscintillators with uniform size and high light yield. In this study, a general and rapid synthesis method is presented that can achieve large-scale preparation of monodisperse and uniform silicate nanoscintillators. By simply adjusting the metal dopants, silicate nanoscintillators with controllable size and X-ray-excited optical luminescence (450-900 nm) are synthesized by employing a general ion-incorporated silica-templating method. To make full use of external radiation, the silicate nanoscintillators are conjugated with photosensitizer rose bengal and arginylglycylaspartic acid (RGD) peptide, making them intrinsically dual-modal targeted imaging probes. Both in vitro and in vivo experiments demonstrate that the silicate nanosensitizers can accumulate effectively in tumors and achieve significant inhibitory effect on tumor progression under low-dose X-ray irradiation, while minimally affecting normal tissues. The insights gained in this study may provide an attractive route to synthesize nanosensitizers to overcome some of the limitations of RT and PDT in cancer treatment.


Assuntos
Nanopartículas/química , Fotoquimioterapia/métodos , Fármacos Fotossensibilizantes/síntese química , Silicatos/química , Animais , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Corantes Fluorescentes/química , Xenoenxertos , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Oligopeptídeos/química , Imagem Óptica , Permeabilidade , Fármacos Fotossensibilizantes/administração & dosagem , Rosa Bengala/química , Raios X
16.
Ecotoxicol Environ Saf ; 175: 243-250, 2019 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-30903880

RESUMO

Multiple heavy metals pollution in environment and food has become an ever-increasing concern and poses a serious threat towards humans and animals. To broad the multiple heavy metals detection, Fe3O4 nanoparticles, Fe3O4/multi-walled carbon nanotubes (Fe3O4/MWCNTs) and Fe3O4/fluorinated multi-walled carbon nanotubes (Fe3O4/F-MWCNTs) nanocomposites were synthesized by hydrothermal method and constructed as a simultaneous electrochemical sensor, respectively. Compared the catalytic performances of the three electrochemical sensors for the simultaneous detection of Cd2+, Pb2+, Cu2+, and Hg2+, the results showed that the Fe3O4/F-MWCNTs sensor demonstrated preponderant performance. It showed the sensitivity of 108.79, 125.91, 160.85, and 312.65 µA mM-1 cm-2 toward Cd2+, Pb2+, Cu2+, and Hg2+, respectively, which was obviously higher than that of Fe3O4/MWCNTs and Fe3O4. Additionally, the Fe3O4/F-MWCNTs sensor exhibited the wider linear detection ranges of 0.5-30.0, 0.5-30.0, 0.5-30.0, and 0.5-20.0 µM for Cd2+, Pb2+, Cu2+, and Hg2+, respectively. The limit of detections of the Fe3O4/F-MWCNTs sensor were 0.05, 0.08, 0.02, and 0.05 nM (signal to noise ratio of 3) for Cd2+, Pb2+, Cu2+, and Hg2+, respectively, fulfilling the governmental requests of the World Health Organization, China and Indian. The excellent agreement was recorded between the lowcost Fe3O4/F-MWCNTs sensor and typical methods (inductively coupled plasma mass spectrometry or atomic fluorescence spectrometry) in river water and soybean samples. Additionally, the sensor also exhibited excellent performances in selectivity, recovery, stability, and reproducibility. This proposal sensor provides a promising strategy to monitor multiple targets in the environment and food.


Assuntos
Técnicas Eletroquímicas/métodos , Poluentes Ambientais/análise , Contaminação de Alimentos/análise , Nanopartículas de Magnetita/química , Metais Pesados/análise , Nanocompostos/química , Nanotubos de Carbono/química , Catálise , Análise Custo-Benefício , Técnicas Eletroquímicas/economia , Eletrodos , Halogenação , Humanos , Limite de Detecção , Reprodutibilidade dos Testes , Propriedades de Superfície
17.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 27(1): 221-226, 2019 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-30738474

RESUMO

OBJECTIVE: To investigate the effects of exosomes from human umbilical cord mesenchymal stem cells on the development of Treg and TH17 cells. METHODS: Exosomes from the serum-free-culture supernatants of hUC-MSC were harvested by ultracentrifugation. The electron microscopy, nanoparticle tracking analysis and western blot were used to identify the hUC-MSC-exosomes, such as the morphology, the paticle chameter, and the protein content. The PBMC stimulated with anti-CD3/CD28 were incubated with the exosomes for five days, and then the percentage changes of Treg and TH17 cells were analyzed by using flow cytometry. RESULTS: The hUC MSC-derived exosomes were saucer-like in morphology the averge diameter was approximately 142 nm. They were identified as positive for CD9 and CD63. Flow cytometry showed that the proportion of CD4+CD25+Foxp3+ Treg cells in the PBMC were significantly higher, but the proportion of CD4+IL17A+ T cells in the hUC-MSC-exosome group was obviously lower than that in the group without the hUC-MSC-exosom (control group) (P<0.05). CONCLUSION: The hUC-MSC-exosomes have an immunomodulatory effect on T cells in vitro by increasing the ratio of Treg and reducing the ratio of TH17 cells, expecting the hUC-MSC-exosom as a novel cell-free target for immunotherapy.


Assuntos
Exossomos , Células-Tronco Mesenquimais , Humanos , Leucócitos Mononucleares , Linfócitos T Reguladores , Células Th17 , Cordão Umbilical
18.
J Chromatogr A ; 1592: 183-187, 2019 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-30704772

RESUMO

A novel dispersive liquid-liquid microextraction method based on temperature-sensitive polymer-induced phase separation was assessed for the determination of phenol, 2,4-dichlorophenol (2,4-DCP), ß-naphthol, and bisphenol A. The interaction between ß-cyclodextrin-poly (N-isopropylacrylamide) and 2,4-DCP was investigated. The results showed that there was an interaction between 2,4-DCP and the polymer with coiled structures and globular structures. The parameters that affected the extraction of phenols were optimized for the peak area. Under optimal conditions, the correlation coefficients were in the range of 0.9828-0.9990. The limits of detection for all the analytes varied from 0.01 to 1.0 µg L-1, and the limits of quantification were between 0.04 and 3.50 µg L-1. The established method was applied successfully to analyze three moat river samples. The relative recoveries were between 81.3 and 117.3%, and the relative standard deviations were less than 17%.


Assuntos
Monitoramento Ambiental/métodos , Cromatografia Gasosa-Espectrometria de Massas , Microextração em Fase Líquida , Fenóis/análise , Temperatura Ambiente , Limite de Detecção , Rios/química
19.
Artigo em Inglês | MEDLINE | ID: mdl-30660987

RESUMO

Cellular signaling initiated by various secreted, cysteine-rich Wnt proteins plays essential roles in regulating animal development and cell stemness. By virtue of its functional diversity and importance, the Wnt gene family has received substantial research interests in a variety of animal species, from invertebrates to vertebrates. However, for bivalve molluscs, one of the ancient bilaterian groups with high morphological diversity, systematic identification and analysis of the Wnt gene family remain lacking. To shed some light on the evolutionary dynamics of this gene family and obtain a more comprehensive understanding, we analyzed the characteristics of the Wnt gene family in three bivalve molluscs, with both genome and extensive transcriptomic resources. Investigation of genomic signatures, functional domains as well as phylogenetic relationships was conducted, and 12, 11, 12 subfamilies were identified in Yesso scallop, Zhikong scallop and Pacific oyster respectively. Spatiotemporal expression profiling suggested that, some bivalve Wnts might coordinate and participate in adult organ/tissue morphogenesis and homeostasis as well as early embryonic development. The transcriptional regulation of oyster Wnt genes showed dynamic and responsive patterns under different environmental stresses, indicating that Wnts may play a role in coping with challenging intertidal environments in bivalves. To our best knowledge, this study presents the first genome-wide study of Wnt gene family in bivalves, and our findings would assist in better understanding of Wnt function and evolution in bivalve molluscs.


Assuntos
Perfilação da Expressão Gênica , Estudo de Associação Genômica Ampla , Moluscos/genética , Proteínas Wnt/genética , Animais , Evolução Biológica , Moluscos/classificação , Filogenia
20.
Toxins (Basel) ; 11(1)2019 01 20.
Artigo em Inglês | MEDLINE | ID: mdl-30669515

RESUMO

Multi-class chemical contaminants, such as pesticides and mycotoxins, are recognized as the major risk factors in agro products. It is thus necessary to develop rapid and simple sensing methods to fulfill the on-site monitoring of multi-class chemical contaminants with different physicochemical properties. Herein, a lateral flow immunoassay via time-resolved fluorescence was developed for the rapid, on-site, simultaneous, and quantitative sensing aflatoxin B1 (AFB1), zearalenone (ZEA), and chlorothalonil (CTN) in maize and peanut. The sample preparation was optimized to a single step, combining the grinding and extraction. Under optimal conditions, the sensing method lowered the limits of detection (LOD) to 0.16, 0.52, and 1.21 µg/kg in maize and 0.18, 0.57, and 1.47 µg/kg in peanut with an analytical range of 0.48⁻20, 1.56⁻200, and 3.63⁻300 µg/kg for AFB1, ZEA and CTN, respectively. The protocol could be completed within 15 min, including sample preparation and lateral flow immunoassay. The recovery range was 83.24⁻110.80%. An excellent correlation was observed between this approach and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for mycotoxins and gas chromatography-tandem mass spectrometry (GC-MS/MS) for pesticide in maize and peanut. This work could be applied in on-site multi-class sensing for food safety.

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