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1.
J Hazard Mater ; 417: 126099, 2021 09 05.
Artigo em Inglês | MEDLINE | ID: mdl-34229391

RESUMO

To improve the harmless treatment of high-concentration heavy metals (HMs) in electroplating sludge (ES), this study tried to combine the microwave pyrolysis technology and the addition of municipal sewage sludge (MS) to synergistically improve the immobilization of high-concentration HMs in ES. The results showed that the immobilization rate of HMs was less than 75% in ES pyrolysis biochar. Notably, the immobilization rate of HMs up to 98.00% in co-pyrolysis biochar. Finally, it was found by various characterizations that the organic carbon and inorganic minerals in MS played an important role in the immobilization of HMs through physical and chemical effects. HMs reacted with inorganic minerals to form HMs crystalline minerals (e.g., CuCl, Cu2NiSnS4, and NiSi2, ZnS) to realize the immobilization of HMs. The addition of organic carbon was conducive to the formation of biochar with higher carbon crystallinity (ID/IG = 0.96) and larger specific surface area (52.50 m2 g-1), thereby enhancing the physical adsorption to HMs. Meanwhile, the complexation reaction between HMs and functional groups such as -OH, Si-O-Si could also further improve the immobilization of HMs. Therefore, this study provided a technical and theoretical basis for the harmless disposal of waste containing multiple HMs with high-concentrations.

2.
Molecules ; 26(14)2021 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-34299445

RESUMO

The thermochemical and kinetic behavior of co-combustion of coal, municipal sludge (MS) and their blends at different ratios were investigated by thermogravimetric analysis. Simulation experiments were performed in a vacuum tube furnace to determine the conversion behavior of toxic elements. The results show that the combustion processes of the blends of coal and municipal sludge are divided into three stages and the combustion curves of the blends are located between those of individual coal and municipal sludge samples. The DTGmax of the sample with 10% sludge addition reaches a maximum at the heating rate of 20 °C/min, indicating that the combustion characteristics of coal can be improved during co-combustion. Strong interactions were observed between coal and municipal sludge during the co-combustion. The volatilization rates of toxic elements decrease with an increasing proportion of sludge in the blends during co-combustion, which indicates that the co-combustion of coal and sludge can effectively reduce the volatilization rate of toxic elements. The study reflects the potential of municipal sludge as a blended fuel and the environmental effects of co-combustion of coal and municipal sludge.

3.
Org Lett ; 2021 Jul 12.
Artigo em Inglês | MEDLINE | ID: mdl-34251839

RESUMO

A series of dicyanomethylene-functionalized triarylboranes is reported in this work, with low-lying LUMO energy levels at ca. -3.66 eV for FMesB-ACN. The single-crystal structures of the mono- and dianion of Mes*B-ACN were obtained via chemical reduction, which revealed a conversion from a quinoidal to an aromatic structure. The strong Lewis acidity of these compounds is reflected in a fluoride-anion binding experiment. This work introduces a facile strategy for modulating the electron deficiency of boron-containing compounds.

4.
J Chem Phys ; 154(22): 224705, 2021 Jun 14.
Artigo em Inglês | MEDLINE | ID: mdl-34241237

RESUMO

Since surface-enhanced Raman scattering (SERS) is of considerable interest for sensing applications in aqueous solution, the role that solvent plays in the spectroscopy must be understood. However, these efforts are hindered due to a lack of simulation approaches for modeling solvent effects in SERS. In this work, we present an atomistic electrodynamics-quantum mechanical method to simulate SERS in aqueous solution based on the discrete interaction model/quantum mechanical method. This method combines an atomistic electrodynamics model of the nanoparticle with a time-dependent density functional theory description of the molecule and a polarizable embedding method for the solvent. The explicit treatment of solvent molecules and nanoparticles results in a large number of polarizable dipoles that need to be considered. To reduce the computational cost, a simple cut-off based approach has been implemented to limit the number of dipoles that need to be treated without sacrificing accuracy. As a test of this method, we have studied how solvent affects the SERS of pyridine in the junction between two nanoparticles in aqueous solution. We find that the solvent leads to an enhanced SERS due to an increased local field at the position of the pyridine. We further demonstrate the importance of both image field and local field effects in determining the enhancements and the spectral signatures. Our results show the importance of describing the local environment due to the solvent molecules when modeling SERS.

5.
Medicine (Baltimore) ; 100(28): e26640, 2021 Jul 16.
Artigo em Inglês | MEDLINE | ID: mdl-34260561

RESUMO

INTRODUCTION: In recent years, with the development of molecular epidemiology, molecular transmission networks based on evolutionary theory and sequence analysis have been widely used in research on human immunodeficiency virus (HIV)-1 transmission dynamics and precise intervention for high-risk populations. The HIV-1 molecular transmission network is a new method to study the population's access to the network, the characteristics of clustering, and the characteristics of interconnection in the network. Here, we analyzed the characteristics of the HIV-1 molecular transmission network of sexually transmitted people in Liaoning Province. METHODS: A study of HIV-infected persons who were sexually transmitted in Liaoning Province from 2003 to 2019. HIV-1 RNA was extracted, amplified and sequenced, and a phylogenetic tree was constructed to determine the subtype using the well matched pol gene region sequence. The gene distance between sequences was calculated, the threshold was determined, and the molecular transmission network was constructed. RESULTS: 109 samples of pol gene region were obtained. The main subtype of HIV-1 was CRF01_AE, followed by B, CRF07_BC, etc. 12.8% of them were resistant to HIV. At the threshold of 0.55 gene distance, 60.6% of them entered the HIV-1 molecular transmission network. Workers, sample source voluntary counseling and testing, other testing, subtype B and drug resistance are the factors influencing the access to HIV-1 molecular transmission network. The subtype of CRF01_AE formed 6 clusters in the molecular transmission network. In the network, the difference of connection degree between different subtypes was statistically significant. DISCUSSION: The three subtypes CRF01_AE, CRF07_BC and B that enter the molecular transmission network do not have interconnections, and they form clusters with each other. It shows that the risk of transmission among the three subtypes is less than the risk of transmission within each subtype. The factors affecting HIV-1 entry into the molecular transmission network were occupation, sample source, genotype and drug resistance. The L33F mutation at the HIV-1 resistance mutation site constitutes the interconnection in the largest transmission cluster in the network. The epidemiological characteristics of HIV-infected persons in each molecular transmission cluster show that 97% of the study subjects come from the same area and have a certain spatial aggregation. CONCLUSION: Constructing a molecular transmission network and conducting long-term monitoring, while taking targeted measures to block the spread of HIV can achieve precise prevention and control.

6.
Artigo em Inglês | MEDLINE | ID: mdl-34297193

RESUMO

INTRODUCTION: The low sensitivity of [18F]-fluorodeoxyglucose ([18F]-FDG) for the diagnosis of gastric cancer limits its application. In this study, we aimed to investigate the potential advantage of [68 Ga]Ga-FAPI-04 over [18F]-FDG in the evaluation of gastric cancer. METHODS: This was a bicentric retrospective analysis of a prospective parent study (clinical trial: HS-KY-2020-826 (Huashan Hospital) and DF-2020-102 (Shanghai East Hospital)). Thirty-eight patients with gastric cancer (31 with adenocarcinoma and 7 with signet ring cell carcinoma) were included in this study. All of the participants underwent [68 Ga]Ga-FAPI-04 and [18F]-FDG imaging by positron emission tomography (PET)/computed tomography (CT) or PET/magnetic resonance (MR). The scans were interpreted by two experienced nuclear medicine physicians, and the maximum standardized uptake value (SUVmax) was calculated. Histopathological findings obtained from biopsy or resected surgical specimens were used as a reference for the final diagnosis. RESULTS: For the detection of primary gastric cancer, the sensitivities of [68 Ga]Ga-FAPI-04 PET and [18F]-FDG PET were 100% (38/38) and 82% (31/38), respectively (P = 0.016). Four cases of adenocarcinoma and three cases of signet ring cell carcinoma were missed by [18F]-FDG PET. The mean SUVmax of [68 Ga]Ga-FAPI-04 in tumours greater than 4 cm (11.0 ± 4.5) was higher than that in tumours less than 4 cm (4.5 ± 3.2) (P = 0.0015). The mean SUVmax of [68 Ga]Ga-FAPI-04 was higher in T2-4 tumours (9.7 ± 4.4) than in T1 tumours (3.1 ± 1.5) (P = 0.0002). For the detection of metastatic lesions, the sensitivities of [68 Ga]Ga-FAPI-04 PET and [18F]-FDG PET in 10 patients with regional lymph node metastasis and distant metastasis were 6/10 and 5/10, respectively. CONCLUSION: In this selected cohort, [68 Ga]Ga-FAPI-04 PET had a superior detection rate than [18F]-FDG PET for primary gastric cancer. [68 Ga]Ga-FAPI-04 PET could provide better performance with regard to gastric cancer diagnosis and staging. Prospective clinical trials are warranted.

7.
Sci Rep ; 11(1): 14842, 2021 Jul 21.
Artigo em Inglês | MEDLINE | ID: mdl-34290362

RESUMO

Little is known regarding the association between depressive symptoms and metabolic syndrome (MetS) among older Chinese adults. This study aimed to examine the association of depressive symptoms with MetS and its components among Chinese elderly. Based on whether they showed depressive symptoms at baseline, 262 age-gender-matched participants from a community-based cohort study were included. The presence of depressive symptoms was measured using the nine-item Patient Health Questionnaire (PHQ-9). MetS was defined according to the Adult Treatment Panel III of the National Cholesterol Education Program. Linear regression and logistic regression analyses were performed to assess associations of depressive symptoms with MetS and its components. The incidence of MetS among the participants with depressive symptoms at baseline was 15.27% (20/131). The association of the presence of depressive symptoms with MetS was significant (odds ratio [OR] = 2.53, 95% confidence intervals [CI] = 1.07, 5.95). There was a negative association between depressive symptoms and hypertension (OR = 0.04, 95% CI = 0.002, 0.98). The change in mean arterial pressure varies approximately 1.03 mmHg with a 1-point change in PHQ-9 score. In this study, baseline depressive symptoms were associated with subsequent MetS. The presence of depressive symptoms was negatively associated with elevated mean arterial pressure.

8.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 38(7): 690-693, 2021 Jul 10.
Artigo em Chinês | MEDLINE | ID: mdl-34247380

RESUMO

OBJECTIVE: To explore the phenotypic and genetic characteristics of acute megakaryoblastic leukemia (AMKL) in young children accompany by WT1, MLL-PTD and EVI1, in order to improve the diagnosis level of AMKL. METHODS: EDTA-K2 anticoagulation venous blood was collected for blood routine and morphological analysis of blood cells; bone marrow was extracted for cell morphology, immunophenotype, chromosome karyotype and fusion gene analysis. RESULTS: White blood cell count was 12.3× 109/L, hemoglobin was 73 g/L, and platelet count was 13× 109/L. The morphological analysis of blood cells showed that the size of immature cells was like that of primitive immature lymphocytes, which was circular or irregular and part of them with obvious pseudopodia. The cytoplasm is basophilic with heterogeneous coloration and granules. Nuclear chromatin is fine and even, 1-3 nucleoli can be seen, these immature cells account for about 40%; the morphology of bone marrow cells was consistent with acute leukemia, negative for peroxidase staining, negative for AS-DNCE staining and alpha-NBE staining. Flow cytometry results showed that the protocells account for about 52% and significant expression of megakaryocytes related markers (cCD41+, CD61+, CD36+). Chromosome karyotype is 46, XX, der(3) add(3)(p21)add(3)(q25), add (9)(q22), -13, +mar [4]/46, XX, del(13)(q12q22) [3]/46, XX[3]. The fusion gene WT1 was overexpressed, MLL-PTD and EVI1 were positive. CONCLUSION: Acute megakaryocytic leukemia has unique and complex phenotypic and genetics characteristics.


Assuntos
Leucemia Megacarioblástica Aguda , Medula Óssea , Criança , Pré-Escolar , Aberrações Cromossômicas , Humanos , Cariotipagem , Leucemia Megacarioblástica Aguda/genética , Megacariócitos , Proteínas WT1
9.
Annu Rev Anal Chem (Palo Alto Calif) ; 14(1): 363-387, 2021 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-34314224

RESUMO

As one of the major types of biomacromolecules in the cell, glycans play essential functional roles in various biological processes. Compared with proteins and nucleic acids, the analysis of glycans in situ has been more challenging. Herein we review recent advances in the development of methods and strategies for labeling, imaging, and profiling of glycans in cells and in vivo. Cellular glycans can be labeled by affinity-based probes, including lectin and antibody conjugates, direct chemical modification, metabolic glycan labeling, and chemoenzymatic labeling. These methods have been applied to label glycans with fluorophores, which enables the visualization and tracking of glycans in cells, tissues, and living organisms. Alternatively, labeling glycans with affinity tags has enabled the enrichment of glycoproteins for glycoproteomic profiling. Built on the glycan labeling methods, strategies enabling cell-selective and tissue-specific glycan labeling and protein-specific glycan imaging have been developed. With these methods and strategies, researchers are now better poised than ever to dissect the biological function of glycans in physiological or pathological contexts.

10.
Ying Yong Sheng Tai Xue Bao ; 32(6): 2180-2190, 2021 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-34212624

RESUMO

Due to its vulnerability and anthropogenic disturbance, ecological problems in karst areas are prominent, such as vegetation destruction, soil erosion, and rocky desertification. Comprehensive analysis of ecological vulnerability and influencing factors in karst areas can provide scientific support for regional ecological restoration and environmental governance. With Guangnan County, a typical karst region in southeastern Yunnan, as an example, we constructed a karst regional eco-environmental vulnerability assessment index system from the perspective of natural and factitious factors. We used SPCA to assess the ecological vulnerability in 2000, 2010 and 2018. We further analyzed the temporal and spatial variations and explored its influencing factors by using geographic detectors. From 2000 to 2018, the changes of overall fragility were small, but the degree of fragility had been intensifying. The grade of ecological vulnerability was mainly slight fragile. The area with mild, moderate and severe fragility was increasing, while the area of extremely fragile showed no change. Guangnan County had a higher ecological vulnerability in the south of "Zhetu-Liancheng-Yangliujing-Banbang", and lower in the north. The spatial agglomeration effect of vulnerability was strong in this county. The north part of Guangnan was vulnerable low-low agglomeration areas, while the southwest and southeast parts were in high-fragility-high agglomeration areas. The implementation of ecological engineering was conducive to the improvement of regional ecological fragility, while the disturbance of human activities further deteriorated ecological fragility. The area proportion of rocky desertification and stratum lithology had stronger influence on ecological fragility of the karst area. The most important influencing factor of the karst ecological fragility was the development of karst carbonate rocks.


Assuntos
Conservação dos Recursos Naturais , Ecossistema , China , Política Ambiental , Atividades Humanas , Humanos
11.
J Texture Stud ; 2021 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-34101194

RESUMO

The influence of high-temperature treatment (100-120°C, 15 min) on the texture, color, and water-holding capacity of tofu gels was investigated. As the temperature increasing, the hardness and chewiness as well as the values of redness a and yellowness b increased gradually, while the water content and the lightness L value reduced progressively, and these variations were more pronounced at 115 or 120°C. Low field nuclear magnetic resonance showed that the loss of T22 water led to the decrease of the water content. Scanning electron microscope revealed that the micropore in gels decreased after heating, and almost entirely disappeared at 120°C. Further analysis by SDS-PAGE indicated the soy protein aggregation formed via disulfide linkage was observed in the thermal treated tofu gels, and nondisulfide linkage might also be occurred as temperature reached 110°C or higher. The quality deterioration may be attributed to immobilized water loss combined with the protein aggregation.

12.
Neurosurg Rev ; 2021 Jun 22.
Artigo em Inglês | MEDLINE | ID: mdl-34159471

RESUMO

It is controversial whether there is a different risk of recurrence between two histological subtypes in craniopharyngioma (CP) patients. Some reported that adamantinomatous craniopharyngioma (ACP) had a higher risk of recurrence than papillary craniopharyngioma (PCP), but others reported that there is no significant difference between them. So, we conducted this systematic review and meta-analysis to determine the association between the histological subtype of CP and the rate of recurrence. A comprehensive literature search was undertaken in PubMed, EMBASE, and Web of Science for all English articles published up to November 2020. Recurrence data stratified by ACP and PCP were extracted from studies meeting inclusion criteria. A pooled analysis of the association between the histological subtype of craniopharyngioma and rates of recurrence was performed. Thirteen articles containing 974 patients were included. When stratified by two pathological subtypes, the total recurrence rate of ACP was 26.0% and PCP was 14.1%, which showed ACP associated with a higher risk of tumor recurrence than PCP (odds ratio [OR] = 2.12, 95% confidence interval [CI] = 1.36, 3.30, P = 0.00). This is the first meta-analysis focusing on histological subtypes of CP. PCP associates with a lower risk of recurrence than ACP, indicating that ACP could act as one of recurrence risk factors for CP patients. Nevertheless, large sample size and well-designed multicenter studies in which the other clinical variables are controlled to determine the histological subtype of CP as an independent recurrence risk factor are needed.

13.
Artigo em Inglês | MEDLINE | ID: mdl-34160025

RESUMO

AIMS: Myocardial fibrosis is associated with clinical ventricular tachyarrhythmia (VTA) events in patients with non-ischaemic dilated cardiomyopathy (DCM). Subepicardial or mid-wall ring-like late gadolinium enhancement (LGE) has received increasing attention in recent years. The aim of this study was to investigate the relationship between ring-like LGE and VTAs in DCM. METHODS AND RESULTS: Patients diagnosed with non-ischaemic DCM who underwent cardiac magnetic resonance with LGE imaging at baseline were investigated. The composite outcome was the occurrence of VTAs defined as sustained ventricular tachycardia, ventricular fibrillation/flutter, aborted sudden cardiac death (SCD), SCD, and appropriate implantable cardioverter-defibrillator intervention. The final cohort comprised 157 patients, including 36 (22.9%) in no LGE group, 48 (30.6%) in focal LGE group, 40 (25.5%) in multi-focal LGE group, and 33 (21%) in ring-like LGE group. Ring-like LGE group patients were younger compared to focal and multi-focal LGE group (P < 0.001) with higher left ventricular ejection fraction (33.0% vs. 24.4% vs. 22.1%, P < 0.001). After a median of 13 ± 7 months follow-up, compared to patients with no LGE, the hazard ratios (HRs) with 95% confidence intervals (CIs) for VTAs were 2.90 (0.56-15.06), 5.55 (1.21-25.44), and 11.75 (2.66-51.92) for patients with focal LGE, multi-focal LGE, and ring-like LGE, respectively. After multivariable adjustment, ring-like LGE group remained associated with increased risk of VTAs (adjusted HR 10.00, 95% CI 1.54-64.98; P = 0.016) independent of the global LGE burden. CONCLUSION: The ring-like pattern of LGE is independently associated with an increased risk of VTAs in patients with non-ischaemic DCM.

14.
Drug Des Devel Ther ; 15: 2529-2541, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34163139

RESUMO

Background: An efficient, fast and sensitive ultra high-performance liquid chromatography-mass spectrometry (UPLC-MS/MS) method for simultaneous determination of celecoxib (CEL), dezocine (DEZ) and dexmedetomidine (DEX) in beagle plasma were established. Methods: The beagle dogs plasmawas precipitated by acetonitrile. The column was Acquity UPLC BEH C18 column and the mobile phase was acetonitrile-formic acid with gradient mode, and the flow rate was set at 0.4 mL/min. Under the positive ion mode, CEL, DEZ, DEX and Midazolam (internal standard, IS) were monitored by multiple reaction monitoring (MRM) as the following mass transition pairs: m/z 381.10→282.10 for CEL, m/z 246.20→147.00 for DEZ, m/z 201.10→94.90 for DEX, and m/z 326.10→291.10 for IS. Results: This UPLC-MS/MS method had good linearity for CEL, DEZ and DEX. The RSDs of inter-day and intra-day precision were the values of 0.31-7.66% and 0.11-9.63%, respectively; the RE values were from -6.05% to 10.98%. The extraction recovery was more than 79%, and the matrix effect was around 100%. The RSDs of stability were less than 8.96%. All of them met the acceptance standard of biological analysis method recommended by FDA. Conclusion: This UPLC-MS/MS method is an effective tool for the simultaneous determination of CEL, DEX and DEX, and has been successfully applied to the study of pharmacokinetics in beagle dogs.

15.
ACS Chem Biol ; 2021 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-34161081

RESUMO

Mammalian cell cycle is a central process for tissue growth and maintenance. Protein O-linked ß-N-acetylglucosamine (O-GlcNAc) modification has been found to occur on several important cell cycle regulators. However, the O-GlcNAcylated proteome has not been extensively profiled during cell cycle progression. Herein, we report a quantitative profiling of protein O-GlcNAcylation sites in cell proliferation, by using an O-GlcNAc chemoproteomic strategy. In HeLa cells, a total of 902, 439, and 872 high-confidence O-GlcNAcylation sites distributed on 414, 265, and 425 proteins are identified in the interphase, early mitosis, and mitotic exit stages, respectively. The identified O-GlcNAcylation events occur on a variety of important regulators, which are involved in the processes of cell division, DNA repair, and cell death. Furthermore, we show that O-GlcNAcylation is dynamically regulated in a cell cycle stage-dependent manner. Our results provide a valuable resource for investigating the functional roles of O-GlcNAc in the mammalian cell cycle.

16.
Cell ; 2021 Jun 09.
Artigo em Inglês | MEDLINE | ID: mdl-34147139

RESUMO

Despite the discovery of animal coronaviruses related to SARS-CoV-2, the evolutionary origins of this virus are elusive. We describe a meta-transcriptomic study of 411 bat samples collected from a small geographical region in Yunnan province, China, between May 2019 and November 2020. We identified 24 full-length coronavirus genomes, including four novel SARS-CoV-2-related and three SARS-CoV-related viruses. Rhinolophus pusillus virus RpYN06 was the closest relative of SARS-CoV-2 in most of the genome, although it possessed a more divergent spike gene. The other three SARS-CoV-2-related coronaviruses carried a genetically distinct spike gene that could weakly bind to the hACE2 receptor in vitro. Ecological modeling predicted the co-existence of up to 23 Rhinolophus bat species, with the largest contiguous hotspots extending from South Laos and Vietnam to southern China. Our study highlights the remarkable diversity of bat coronaviruses at the local scale, including close relatives of both SARS-CoV-2 and SARS-CoV.

17.
Ultrason Sonochem ; 76: 105652, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34182317

RESUMO

The effects of the different combined manner of ultrasound and covalent reaction between polyphenol and myofibrillar protein (MP) from chicken were studied. More so, antioxidant activities, digestive properties, and potential mechanism of ultrasound-assisted oxidation system of hydrophilic ((-)-Epicatechin gallate, ECG) and hydrophobic (Baicalein, BN) polyphenols was also analyzed in this study. Among all the combined treatments, surface hydrophobicity (SUH), active sulfhydryl contents (ASC), and specific surface area (SSA) of ultrasonic assisted ECG oxidation group (T6) was relatively apparent, indicating that a more unfolding MP structure was obtained. Furthermore, ultrasonic assisted ECG oxidation group showed the highest antioxidant activities compared with other combined treatments on the basis of the results of DPPH free radical scavenging activities, metal ion chelating activities, and hydroxyl radicals (OH·) scavenging activities. The results of simulated digestion system and kinetic analysis also verified that ultrasonic assisted ECG oxidation had higher MP bio-accessibility than the control group. In contrast, a lower digestibility was displayed in ultrasonic assisted BN oxidation group. In summary, the ultrasound-assisted covalent reaction of MP and ECG might be a desirable approach for industrial production of MP from chicken with better antioxidant activities and digestive properties.

18.
J Affect Disord ; 292: 255-260, 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34134023

RESUMO

BACKGROUND: Increasing evidence has demonstrated that childhood adversity was a predictor of pain and hypothalamic-pituitary-adrenal (HPA) axis genetic variation is associated with pain risk. This study aims to explore possible effects of prolonged childhood separation from parents and HPA polygenic risk score (PRS) on pain among adolescents in rural China. METHOD: We used data from 219 adolescents in rural area of Fuyang city, Anhui province, China. Parent-child separation was collected through interview and pain intensity was reported using the 11-point Numerical Rating Scale. SNP genotyping was performed using an improved multiplex ligation detection reaction (iMLDR) technique. The PRS was computed based on 3 single nucleotide polymorphisms (SNPs) in 2 genes (FKBP5 and NR3C1) related to HPA-axis stress reactivity. RESULTS: Pain among adolescents separated from both parents scored higher compared to those without parent-child separation, however, this association was only observed in adolescents with moderate to high tertiles of PRS groups (parent-child separation in moderate group vs. no parent-child separation in moderate group: 3.07 vs. 1.57, P < 0.001; parent-child separation in highest group vs. no parent-child separation in highest group: 3.02 vs. 1.26, P < 0.001; parent-child separation in lowest group vs. no parent-child separation in lowest group: 2.34 vs. 1.25, P = 0.225). After controlled for demographic characteristics, psychopathological symptoms, adverse childhood experiences, parental warmth, prolonged childhood parent-child separation increased pain scores by 1.52 points (95% CI:0.72, 2.33) and 1.72 points (95% CI:1.13, 2.31) in moderate and high PRS groups, respectively. CONCLUSION: Our findings suggest that adolescents separated from both parents while carrying more risk alleles related to HPA-axis stress reactivity are at heightened risk of pain.

19.
Am J Hum Genet ; 108(7): 1350-1355, 2021 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-34115965

RESUMO

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) causes coronavirus disease 2019 (COVID-19), a respiratory illness that can result in hospitalization or death. We used exome sequence data to investigate associations between rare genetic variants and seven COVID-19 outcomes in 586,157 individuals, including 20,952 with COVID-19. After accounting for multiple testing, we did not identify any clear associations with rare variants either exome wide or when specifically focusing on (1) 13 interferon pathway genes in which rare deleterious variants have been reported in individuals with severe COVID-19, (2) 281 genes located in susceptibility loci identified by the COVID-19 Host Genetics Initiative, or (3) 32 additional genes of immunologic relevance and/or therapeutic potential. Our analyses indicate there are no significant associations with rare protein-coding variants with detectable effect sizes at our current sample sizes. Analyses will be updated as additional data become available, and results are publicly available through the Regeneron Genetics Center COVID-19 Results Browser.


Assuntos
COVID-19/diagnóstico , COVID-19/genética , Exoma/genética , Predisposição Genética para Doença , Hospitalização/estatística & dados numéricos , Sequenciamento Completo do Exoma , COVID-19/imunologia , COVID-19/terapia , Feminino , Humanos , Interferons/genética , Masculino , Prognóstico , SARS-CoV-2 , Tamanho da Amostra
20.
Complement Ther Clin Pract ; 44: 101426, 2021 Jun 09.
Artigo em Inglês | MEDLINE | ID: mdl-34139547

RESUMO

OBJECTIVE: The primary purpose of this study is to structure the available evidence relating to physical exercise programs and their impact on patients' cardiovascular system during the convalescence for breast cancer. METHODS: We searched six English databases and four Chinese databases from inception to May 19, 2021. Two reviewers independently screened literature, extracted data. They assessed the risk of bias according to the eligibility criteria, and the Cochrane Collaboration RevMan 5.3.0 version software and STATA 15.0 software were used for this meta-analysis. This study has been registered in the International Prospective Register of Systematic Reviews (CRD42021226319). RESULTS: In total, 3483 articles were screened and data from 11 randomized controlled trials (RCTs) involving 666 breast cancer patients were used in this meta-analysis. The results showed that exercise could decrease systolic blood pressure (SBP) (P = 0.006), diastolic blood pressure (DBP) (P = 0.0003), triglycerides (TG) levels (P < 0.00001), body mass index (BMI) (P = 0.009). Results also showed that exercise could significantly increase peak oxygen uptake (VO2peak) (P = 0.009), maximal oxygen consumption (VO2max) (P = 0.01), and High-density leptin cholesterol (HDL-C) levels (P < 0.0001). However, compared with the control group, there was no significant changes of mean arterial pressure (MAP), peak heart rate (HRpeak), and peak respiratory exchange ratio (PERpeak) (P > 0.05). CONCLUSIONS: Physical exercise could improve the cardiovascular system function associated with decreased the levels of SBP, DBP, TG, and increased the levels of VO2peak, VO2max, and HDL-C in breast cancer patients. These findings reveal that exercise may be a promising means for cardiovascular nursing.

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