Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 12 de 12
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Artigo em Inglês | MEDLINE | ID: mdl-35446770

RESUMO

With the development of artificial intelligence, speech recognition and prediction have become one of the important research domains with wild applications, such as intelligent control, education, individual identification, and emotion analysis. Chinese poetry reading contains rich features of continuous pronunciations, such as mood, emotion, rhythm schemes, lyric reading, and artistic expression. Therefore, the prediction of the pronunciation characteristics of a Chinese poetry reading is the significance for the presentation of high-level machine intelligence and has the potential to create a high-level intelligent system for teaching children to read Tang poetry. Mel frequency cepstral coefficient (MFCC) is currently used to present important speech features. Due to the complexity and high degree of nonlinearity in poetry reading, however, there is a tough challenge facing accurate pronunciation feature prediction, that is, how to model complex spatial correlations and time dynamics, such as rhyme schemes. As for many current methods, they ignore the spatial and temporal characteristics in MFCC presentation. In addition, these methods are subjected to certain limitations on prediction for long-term performance. In order to solve these problems, we propose a novel spatial-temporal graph model (STGM-MHA) based on multihead attention for the purpose of pronunciation feature prediction of Chinese poetry. The STGM-MHA is designed using an encoder-decoder structure. The encoder compresses the data into a hidden space representation, while the decoder reconstructs the hidden space representation as output. In the model, a novel gated recurrent unit (GRU) module (AGRU) based on multihead attention is proposed to extract the spatial and temporal features of MFCC data effectively. The evaluation comparison of our proposed model versus state-of-the-art methods in six datasets reveals the clear advantage of the proposed model.

2.
Gland Surg ; 11(2): 412-425, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-35284305

RESUMO

Background: Seizure-related 6 homolog (mouse)-like 2 (SEZ6L2) is a type 1 transmembrane protein that is primarily expressed in the brain . In recent reports, SEZ6L2 has been found to be overexpressed in some cancers and can drive the progression of tumors. However, its function and mechanism in thyroid cancer remain unclear. Methods: In this article ,we searched for the SEZ6L2 expressions in Pan-cancer on TCGA (The Cancer Genome Atlas) and evaluated these data using the TIMER2 method. Then, the immunohistochemical score (IHC score) of SEZ6L2 in cancer tissue was collected in human protein mapping (HPA) data. And we used CIBERSORT to assess the association between the levels of SEZ6L2 expression and the number of various immune cells in papillary thyroid carcinoma (PTC) tissue. Finally, Gene Expression Omnibus (GEO) analyses, real-time quantitative polymerase chain reaction (qRT-PCR) of tissues, and immunohistochemical staining were used to detect the result. Results: The article illustrated that a large number of cancers had a higher expression of SEZ6L2 compared to the control tissues. And the immunohistochemical score (IHC score) of SEZ6L2 in cancer tissue was considerably elevated compared to that in normal tissues SEZ6L2 was elevated in thyroid carcinoma (THCA) tissue, besides, GEO analyses, qRT-PCR of tissues, and immunohistochemical staining were conducted to test the results. Finally, the Kaplan-Meier survival analysis illustrated that the increased expression of SEZ6L2 was correlated with a dismal prognosis-higher SEZ6L2 is associated with shorter survival. And the univariate analysis illustrated that T stage, SEZ6L2 and Pathologic stage were related to the overall survival (OS), multivariate analysis stated that elevated expression of SEZ6L2 was an independent risk factor that affected progression-free interval (PFI) (P<0.05). Consequently, we found that the expression of SEZ6L2 was correlated with tumor-infiltrating immune cells by TIMER. Conclusions: SEZ6L2 was upregulated in patients with THCA and that increased expression of SEZ6L2 was related with clinical progression and was regarded as an independent risk factor for PFI. In THCA patients, the expression of SEZ6L2 could be a significant prognostic factor, which is expected to be a prospective biomarker for THCA in the future.

3.
Huan Jing Ke Xue ; 43(1): 230-238, 2022 Jan 08.
Artigo em Chinês | MEDLINE | ID: mdl-34989507

RESUMO

Based on the flow direction of the Fuhe River into Baiyangdian Lake, the impacted area of the Fuhe River was divided into 6 subareas, and sediments from 48 sites were collected in November 2020. The characteristics and risks of sediment nutrients and heavy metal pollution in these six subareas were investigated. The results showed that the average ω(TN), ω(TP), and ω(TOC) were 1841 mg·kg-1, 769 mg·kg-1, and 1.77%, respectively. The major heavy metals were Cd, Cu, Zn, Hg, and Pb, which were 3.73, 1.50, 1.42, 1.31, and 1.31 times the soil background values for Hebei Province, respectively. The TP and heavy metal (Cd, Cu, Zn, Hg, and Pb) content showed a decreasing trend from the Fuhe River estuary to the downstream Zaolinzhuang, whereas the TN and TOC content showed no marked trends. TN, TP, TOC, and heavy metals (Cd, Cu, Zn, Hg, and Pb) were enriched in surface sediments (0-10 cm). The TP content in the surface sediments (0-10 cm) of the Fuhe River estuary, Fuhe River estuary-Nanliuzhuang, and Nanliuzhuang subareas were heavily polluted; the Wangjiazhai and Guangdian subareas were moderately polluted; and the Zaolinzhuang subarea was slightly polluted. Cd and Hg were the major contributors to heavy metal pollution, which were at considerable risk and moderate risk levels, respectively. The heavy metals in surface sediments (0-10 cm) of the Fuhe River estuary, Fuhe River estuary-Nanliuzhuang, and Nanliuzhuang subareas were at a considerable risk level, and the sediments below 30 cm presented a low risk level. The leaching concentrations of heavy metals in sediments from the subarea of severe ecological risk level were far less than the identification standard values of leaching toxicity, suggesting that the sediments can be treated as general waste after dredging.


Assuntos
Metais Pesados , Poluentes Químicos da Água , China , Monitoramento Ambiental , Sedimentos Geológicos , Lagos , Metais Pesados/análise , Nutrientes , Medição de Risco , Poluentes Químicos da Água/análise
4.
Environ Sci Pollut Res Int ; 29(11): 16716-16726, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-34655384

RESUMO

Baiyangdian Lake (BYD), a large shallow lake in North China, has complex water landscape patterns that are underlies spatial variations in water quality. In this study, we collected 61 water samples from three water landscapes (reed littoral zones, fish ponds, and open water) and analyzed them for water quality parameters, such as dissolved organic carbon (DOC), total nitrogen (TN), and total phosphorus (TP). Water landscape distribution (determined using remote sensing imagery) was then used to assess correlations between water quality parameters and water landscape proportion in differently scaled buffer zones. There was substantial variation across all subareas, with TN and TP concentrations ranging from 0.90 to 4.10 mg/L and 0.06 to 0.18 mg/L, respectively, in class IV of water quality as a whole. Spatial variations in water quality were mainly caused by water landscape distribution and external nutrient inputs. There were negative correlations between DOC, TN, and TP concentrations and the area proportion of reed littoral zones in the 300 and 500 m buffers. In contrast, DOC, TN, and TP concentrations were significantly positively correlated with the area proportion of fish ponds in the 100 m buffer. Furthermore, compared with reed littoral zones, a lower ratio of nitrogen to phosphorus and a higher proportion of dissolved organic nitrogen and tyrosine-like proteins were found in fish ponds. These effects were mainly attributed to the development of internal sediment loadings due to nutrient exchange across the sediment-water interface. Therefore, dredging-based sediment removal from fish ponds should be considered to suppress internal phosphorus loading and accelerate recovery of the BYD ecosystem.


Assuntos
Lagos , Poluentes Químicos da Água , China , Ecossistema , Monitoramento Ambiental , Nitrogênio/análise , Fósforo/análise , Poluentes Químicos da Água/análise , Qualidade da Água
5.
Zhonghua Nan Ke Xue ; 27(6): 506-512, 2021 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-34914289

RESUMO

OBJECTIVE: To investigate the correlation of the single nucleotide polymorphisms (SNP) rs1126772, rs117291487, rs11730582, rs142608941 and rs6813526 of the osteopontin (OPN) gene with the risk of asthenozoospermia (AZS). METHODS: We included 135 AZS patients in the AZS group and another 239 fertile men as normal controls. Using the SNaPshot technique, we genotyped the rs1126772, rs117291487, rs11730582, rs142608941 and rs6813526 polymorphisms of the OPN gene in all the subjects and analyzed the correlation of the five SNPs with AZS. RESULTS: The GA genotype and A allele of the OPN gene rs1126772 were found to be correlated with the risk of AZS (GA vs AA: OR = 0.55, 95% CI: 0.35-0.86, P = 0.009; A vs G: OR = 0.64, 95% CI: 0.46-0.89, P = 0.007), and so was the CT genotype and T allele at the RS11730582 locus (CT vs TT: OR = 0.526, 95% CI: 0.34-0.82, P = 0.009; T vs C: OR = 0.60, 95% CI: 0.44-0.83, P = 0.002). Haplotype analysis showed that the AATCT haplotype decreased the risk of AZS (AATCT: OR = 0.61, 95% CI: 0.42-0.88, P = 0.008) . CONCLUSIONS: The polymorphisms of the OPN gene RS1126772 and RS11730582 may reduce the risk of AZS.


Assuntos
Astenozoospermia/genética , Osteopontina , Polimorfismo de Nucleotídeo Único , Humanos , Masculino , Osteopontina/genética
6.
Opt Lett ; 46(24): 6071-6074, 2021 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-34913920

RESUMO

A single-optic-fiber sensor is proposed to simultaneously detect the refractive index (RI) and temperature (T) at a single wavelength band. This sensor is based on the mixed effects of Mach-Zehnder interference (MZI) and surface plasmon resonance (SPR), where MZI is excited by a tapered-fiber structure, and SPR is stimulated by a 45 nm gold film on the tapered-fiber surface. The detection signal of an SPR spectrum superimposed on interference stripes was obtained. After fast Fourier transform and filter processing, the MZI and SPR signals were separated. Experimental results indicate that our sensor can improve the RI sensitivity to 2021.07 nm/RIU (21-fold greater than that of the original tapered-fiber MZI sensor) and detect T simultaneously. Additionally, this highly integrated sensor simplifies the detection system, with potential applications in portable biochemical sensing.


Assuntos
Tecnologia de Fibra Óptica , Refratometria , Temperatura
7.
Huan Jing Ke Xue ; 42(10): 4781-4788, 2021 Oct 08.
Artigo em Chinês | MEDLINE | ID: mdl-34581120

RESUMO

A dredging demonstration project in the Baiyangdian Lake included open waters and fishing ponds to reduce the internal release of nitrogen and phosphorus from bottom sediments. The dredging depth design was determined by both the sediment vertical distribution profile of total nitrogen and phosphorus, and the sediment adsorption-desorption equilibrium method. The determined dredging depths were very similar and coincident. The dredging depth for the demonstration area of open waters in Nanliuzhuang was identified as(50±10) cm; and the dredging depths for fishing ponds were(30±10) cm in both the Nanliuzhuang and Caiputai demonstration areas. The equilibrium nitrogen(NH4+-N) and phosphorus(SRP) concentrations at zero net sorption or desorption(ENC0 and EPC0) were significantly positively correlated with both exchangeable and total nitrogen and phosphorus in the sediments. The total nitrogen and phosphorus in the sediments were also used to predict the risk of their release from the bottom sediments to the overlying water column. The sediment layers with ENC0 and EPC0 values greater than the NH4+-N and SRP in the overlying water column indicated the sediments act as a source of dissolved nitrogen and phosphorus to the overlying water column in the Nanliuzhuang and Caiputai demonstration areas. Accordingly, the sediment layers with both total nitrogen concentrations greater than 750 mg·kg-1 and total phosphorus concentrations greater than 500 mg·kg-1 should be identified as dredging layers.


Assuntos
Fósforo , Poluentes Químicos da Água , Adsorção , China , Sedimentos Geológicos , Lagos , Nitrogênio/análise , Fósforo/análise , Poluentes Químicos da Água/análise
8.
Biomed Opt Express ; 12(8): 5305-5319, 2021 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-34513258

RESUMO

Widely used for medical analysis, the texture of the human scar tissue is characterized by irregular and extensive types. The quantitative detection and analysis of the scar texture as enabled by image analysis technology is of great significance to clinical practice. However, the existing methods remain disadvantaged by various shortcomings, such as the inability to fully extract the features of texture. Hence, the integration of second harmonic generation (SHG) imaging and deep learning algorithm is proposed in this study. Through combination with Tamura texture features, a regression model of the scar texture can be constructed to develop a novel method of computer-aided diagnosis, which can assist clinical diagnosis. Based on wavelet packet transform (WPT) and generative adversarial network (GAN), the model is trained with scar texture images of different ages. Generalized Boosted Regression Trees (GBRT) is also adopted to perform regression analysis. Then, the extracted features are further used to predict the age of scar. The experimental results obtained by our proposed model are better compared to the previously published methods. It thus contributes to the better understanding of the mechanism behind scar development and possibly the further development of SHG for skin analysis and clinic practice.

9.
ACS Appl Mater Interfaces ; 13(35): 42138-42145, 2021 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-34432434

RESUMO

By preparing a series of high-quality Fe1.1Se0.8Te0.2 films on the CaF2 substrate via pulsed laser deposition, we reveal the evolution of the structure as well as the superconductivity with the film thickness. We have found that there exists a threshold thickness above which the critical temperature Tc reaches its optimal value of 23.18 K with large activation energy, promising for high-field technological applications. Most importantly, the thick films have been found in a metastable state due to the fragile balance between the increased strain energy and the large compressive stress. Once the balance is broken by an external perturbation, a unique structure avalanche happens with a large part of the film exfoliated from the substrate and curves out. The exfoliated part of the film remains a single phase, with its lattice parameter and Tc recovering the bulk values. Our results clearly demonstrate the close relation between the compressive stress of the film/substrate interface and the high critical temperature observed in FeSeTe films. Moreover, this also provides an efficient way to fabricate free-standing single-phase FeSeTe crystals in the phase-separation regime.

10.
J Cell Mol Med ; 23(4): 2410-2418, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30632263

RESUMO

Interleukin-27 (IL-27) gene polymorphisms are linked to infectious disease susceptibility and IL-27 plasma level is associated with HIV infection. Therefore, we aimed to investigate the association between IL-27 polymorphisms and susceptibility to HIV infection and disease progression. A total of 300 patients with HIV infection (48 long-term nonprogressors and 252 typical progressors) and 300 healthy controls were genotyped for three IL-27 polymorphisms, rs17855750, rs181206, rs40837 which were performed by using multiple single nucleotide primer extension technique. Significant association was found between IL-27 rs40837 polymorphisms with susceptibility to HIV infection (AG vs AA: adjusted OR = 1.60, 95% CI, 1.11-2.30, P = 0.012; AG+GG vs AA: adjusted OR = 1.44, 95% CI, 1.02-2.03, P = 0.038) and disease progression (LTNP: AG vs AA: adjusted OR = 2.33, 95% CI, 1.13-4.80, P = 0.021; TP: AG vs AA: adjusted OR = 1.50, 95% CI, 1.04-2.24, P = 0.030). Serum IL-27 levels were significantly lower in cases compared to controls (P < 0.001). There were lower serum IL-27 levels in TPs than in LTNPs (P < 0.001). We further found that LTNPs with rs40837 AG or GG genotype had lower serum IL-27 levels than with AA genotype (P < 0.05). The CD4+ T counts in cases were significantly lower than controls (P < 0.001). In contrast, individuals with rs40837 AG genotype had lower CD4+ T counts than with AA genotype in cases (P < 0.05). In addition, CD4+ T counts in TPs were significantly lower than LTNPs (P < 0.001). IL-27 rs40837 polymorphism might influence the susceptibility to HIV infection and disease progression probably by regulating the level of serum IL-27 or the quantity of CD4+ T.


Assuntos
Estudos de Associação Genética , Predisposição Genética para Doença , Infecções por HIV/genética , Interleucinas/genética , Adulto , Progressão da Doença , Feminino , Frequência do Gene , Genótipo , Infecções por HIV/virologia , Humanos , Masculino , Pessoa de Meia-Idade , Polimorfismo de Nucleotídeo Único/genética
11.
Zhonghua Nan Ke Xue ; 24(7): 596-601, 2018 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-30173441

RESUMO

Objective: To investigate the correlation of the single nucleotide polymorphisms (SNPs) rs12009, rs1140763 and rs16927997 in the 3'-untranslated region (3'UTR) of the glucose-regulated protein 78 (GRP78) gene with the risk of male asthenozoospermia (AZS). METHODS: We included 400 AZS patients in the AZS group and another 400 fertile men as normal controls. Using the SNaPshot technique, we genotyped the rs12009, rs1140763 and rs16927997 polymorphisms in the 3'UTR of the GRP78 gene in all the male subjects and analyzed the association of the three SNPs with AZS. RESULTS: The percentage of progressively motile sperm was significantly lower in the AZS group than in the normal controls (ï¼»20.09 ± 8.18ï¼½ % vs ï¼»57.16 ± 13.45ï¼½ %, P <0.01). Three genotypes of CC, CT and TT and 2 alleles of C and T were found in rs12009 and rs1140763 of the GRP78 gene, and another three genotypes of GG, GA and AA and two alleles of G and A were observed in rs16927997. There were no statistically significant differences between the control and AZS groups in the frequencies of the C and T alleles in rs12009 (44.3% vs 47.3% and 55.7% vs 52.7%, P >0.05) or rs1140763 (50.0% vs 52.0% and 50.0% vs 48.0%, P >0.05) or those of the G and A alleles in rs16927997 (6.0% vs 4.4% and 94.0% vs 95.6%, P >0.05), nor in the genotypes and allele frequencies of the 3 polymorphisms (P >0.05). Furthermore, three haplotypes of C-C-A, T-C-G and T-T-A were observed in the male subjects but showed no evident correlation between the AZS and normal control groups. CONCLUSIONS: The polymorphisms in the 3'UTR of the GRP78 gene are not correlated with the risk of male asthenozoospermia.


Assuntos
Regiões 3' não Traduzidas/genética , Astenozoospermia/genética , Proteínas de Choque Térmico/genética , Polimorfismo de Nucleotídeo Único , Alelos , Feminino , Frequência do Gene , Predisposição Genética para Doença , Genótipo , Haplótipos , Humanos , Masculino , Risco
12.
Immunogenetics ; 59(10): 813-21, 2007 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-17891481

RESUMO

Both inhibitory and activating forms of natural killer (NK) cell receptors are found in mammals. The activating receptors play a direct role in the recognition of virally infected or transformed cells and transduce activating signals into the cell by partnering with an adaptor protein, which contains a cytoplasmic activation motif. Activating NK receptors encoded by the mammalian leukocyte receptor complex (e.g., killer cell immunoglobulin-like receptors) and the natural killer complex (e.g., Ly49s) partner with the adaptor protein DAP12, whereas NK receptors encoded in the CD94/NKG2 complex partner with the adaptor protein DAP10. Novel immune-type receptors (NITRs) found in bony fish share several common features with immunoglobulin-type NK receptors. Nitr9 is a putative activating receptor in zebrafish that induces cytotoxicity within the context of human NK cells. One isoform of Nitr9, Nitr9L, is shown here to preferentially partner with a zebrafish ortholog of Dap12. Cross-linking the Nitr9L-Dap12 complex results in activation of the phosphytidylinositol 3-kinase-->AKT-->extracellular signal-regulated kinase pathway suggesting that the DAP12-based activating pathway is conserved between bony fish and mammals.


Assuntos
Receptores KIR/metabolismo , Transdução de Sinais , Proteínas de Peixe-Zebra/metabolismo , Proteínas Adaptadoras de Transdução de Sinal/genética , Proteínas Adaptadoras de Transdução de Sinal/imunologia , Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Sequência de Aminoácidos , Animais , Linhagem Celular , Humanos , Dados de Sequência Molecular , Fosforilação , Receptores KIR/genética , Receptores KIR/imunologia , Proteínas Recombinantes/imunologia , Proteínas Recombinantes/metabolismo , Alinhamento de Sequência , Transdução de Sinais/imunologia , Peixe-Zebra , Proteínas de Peixe-Zebra/genética , Proteínas de Peixe-Zebra/imunologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...