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1.
BMC Pulm Med ; 19(1): 204, 2019 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-31703576

RESUMO

BACKGROUND: Myeloid sarcoma (MS) rarely involves the bronchus, and primary bronchial MS has almost never been reported in mainland China. CASE PRESENTATION: A 65-year-old female patient was admitted with a 3-month history of cough. She was initially diagnosed with bronchogenic carcinoma according to chest computed tomography (CT). However, after a biopsy was taken from the endobronchial lesion by bronchoscopy and further immunohistochemical analysis was performed, the diagnosis of MS was made. Because her bone marrow was normal and she had no history of haematologic diseases, we further considered the diagnosis of primary bronchial MS. The patient received chemotherapy with HAG regimens, and the original mass completely resolved, as confirmed by chest CT scan after 3 cycles of treatment. Meanwhile, no abnormalities were found on re-examination via bronchoscopy. CONCLUSIONS: MS should be considered in the differential diagnosis in the presence of a suspicious pulmonary mass. Immunohistochemical analysis is necessary to confirm the diagnosis. Chemotherapy can lengthen the survival time for patients.


Assuntos
Carcinoma Broncogênico/diagnóstico , Neoplasias Pulmonares/diagnóstico , Sarcoma Mieloide/diagnóstico , Idoso , Antineoplásicos/uso terapêutico , Biópsia , Broncoscopia , Diagnóstico Diferencial , Feminino , Humanos , Neoplasias Pulmonares/terapia , Sarcoma Mieloide/terapia , Tomografia Computadorizada por Raios X
2.
PLoS One ; 14(4): e0203238, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30939172

RESUMO

Agroforestry is a common traditional practice in China-especially in the southern Xinjiang of Northwest China. However, the productivity of many agroforestry systems has been lower than expected in recent years, highlighting the need for an actionably deep mechanistic understanding of the competition between crops and trees. Here, three different fruit tree/wheat (jujube/wheat, apricot /wheat, and walnut /wheat) intercropping agroforestry systems were chosen to investigate influence of different fruit tree shade intensity on the growth, yield and quality of intercropping wheat. Compared to the monoculture wheat system, the mean daily shade intensity of the jujube-, apricot-, and walnut-based intercropping systems were, respectively, 23.2%, 57.5%, and 80.7% shade. The photosynthetic rate of wheat in the jujube-, apricot-, and walnut-based intercropping systems decreased by, respectively, 11.3%, 31.9%, and 36.2% compared to monoculture wheat, and the mean number of fertile florets per spike decreased by 26.4%, 37.4%, and 49.5%. Moreover, the apricot- and walnut-based intercropping systems deleteriously affected grain yield (constituent components spike number, grains per spike, and thousand grain weight) and decreased the total N, P, and K content of intercropping wheat. Tree shading intensity strongly enhanced the grain protein content, wet gluten content, dough development time, and dough stability time of wheat, but significantly decreased the softening degree. Strong negative linear correlations were observed between tree shade intensity and the number of fertile florets, grain yield related traits (including spike number, grains per spike, and thousand grain weight), nutrient content (N, P and K), and softening degree of wheat. In contrast, Daily shade intensity was positively linearly correlated with grain protein content, wet gluten content, dough development time, and dough stability time. We conclude that jujube-based intercropping systems can be practical in the region, as they do not decrease the yield and quality of intercropping wheat.


Assuntos
Produção Agrícola , Frutas , Árvores , Triticum/crescimento & desenvolvimento
3.
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 44(2): 134-143, 2019 Feb 28.
Artigo em Chinês | MEDLINE | ID: mdl-30837381

RESUMO

OBJECTIVE: To investigate the effects of airway epithelial cells on macrophages chemotaxis and inflammatory cytokine expression under hypoxic conditions.
 Methods: Human bronchial epithelial cells (HBE) treated with different concentrations (0, 100, 200, 400, 800 µmol/L) of CoCl2 or transfected with HIF-1α siRNA were co-cultured with THP-1-derived M1 macrophages or M2 macrophages. The chemotactic effects on macrophages were analyzed by Transwell assay. The levels of TNF-α, IFN-γ, IL-4, IL-13 and IL-10 in the supernatants of macrophages were detected by ELISA, and HIF-1α or Cav-1 mRNA expression in HBE or macrophages was detected by RT-qPCR.
 Results: HBE cells promoted macrophages chemotaxis in a time- and concentration-dependent manner. Compared to un-transfected group, the chemotactic ability of HBE transfected with HIF-1α siRNA was significantly weakened (P<0.01). Under the same culture conditions, the chemotaxis of M2 macrophages was greater than that in THP1-derived M1 macrophages. The concentrations of TNF-α, IFN-γ, IL-4, IL-13 and IL-10 in the supernatants of macrophages were increased in a time-and concentration-dependent manner. The concentrations of TNF-α and IFN-γ were increased further after co-culturing for 8 and 12 h; while IL-4, IL-13 and IL-10 concentrations were increased further during 24 h of co-culture. The levels of cytokines in the supernatants of macrophages co-cultured with HBE and transfected with HIF-1α siRNA were significantly lower than those in un-transfected cells (P<0.05 or P<0.01). The reduction of TNF-α or IFN-γ was more obvious. The expression of HIF-1α or Cav-1 mRNA in HBE or macrophages was increased in a concentration-dependent manner after 8 or 12 h co-culture, which was significantly reduced when HBE was transfected with HIF-1α siRNA.
 Conclusion: Airway epithelial cells can enhance macrophages chemotaxis and pro-inflammatory cytokines expressions under hypoxic condition. HIF-1α and Cav-1 may be the important mediators in these processes.


Assuntos
Quimiotaxia , Hipóxia Celular , Citocinas , Células Epiteliais , Humanos , Subunidade alfa do Fator 1 Induzível por Hipóxia , Macrófagos
4.
J Cell Mol Med ; 23(2): 1458-1469, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30515965

RESUMO

Lung cancer (LC) is a devastating malignancy with no effective treatments, due to its complex genomic profile. Using bioinformatics analysis and immunohistochemical of lung carcinoma tissues, we show that TRIM59 as a critical oncoprotein relating to LC proliferation and metastasis. In this study, high TRIM59 expression was significantly correlated with lymph node metastasis, distant metastasis, and tumour stage. Furthermore, up-regulation of TRIM59 expression correlated with poorer outcomes in LC patients. Mechanistically, TRIM59 play a key role in promoting LC growth and metastasis through regulation of extracellular-signal regulated protein kinase (ERK) signalling pathway and epithelial-to-mesenchymal transition (EMT)-markers, as validated by loss-of-function studies. In-depth bioinformatics analysis showed that there is preliminary evidence of co-expression of TRIM59 and cyclin dependent kinase 6 (CDK6) in LC. Notably, CDK6 expression significantly decreased when TRIM59 was knocked down in the LC cells. In contrast, exogenous up-regulation of TRIM59 expression also induced significant increases in the expression of CDK6. Moreover, the expression of CDK6 was also inhibited by the ERK signalling inhibitor, U0126. The results of both loss- and gain-of-function studies showed that TRIM59 could regulate the expression of CDK6. Collectively, these data provide evidence that TRIM59 is involved in lung carcinoma growth and progression possibly through the induction of CDK6 expression and EMT process by activation of ERK pathway.

5.
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 44(12): 1376-1384, 2019 Dec 28.
Artigo em Chinês | MEDLINE | ID: mdl-31969502

RESUMO

OBJECTIVE: To compare the clinical features and the heterogeneity of macrophages in different clinical phenotypes of chronic obstructive pulmonary disease (COPD) patients with frequent or infrequent exacerbations.
 Methods: Clinical characteristics of eighty COPD patients with chronic bronchitis (CB), emphysema (EM) or asthma-COPD overlap (ACO) phenotypes suffered from acute exacerbation were analyzed. The expressions of CCL3 and CD163 in sputum macrophages were detected by flow cytometry. The expressions of HIF-1α and Cav-1 in sputum macrophages were detected by quantitative PCR (qPCR).
 Results: The age, forced expiratory volume in one second (FEV1)/forced vital capacity (FVC), sputum bacteria positive rate, COPD Assessment Test (CAT) score, and Modified Medical Research Council (mMRC) score between the patients with FE and iFE were significantly different (P<0.05). Compared with iFE patients, the fluorescence intensity of CCL3 in sputum macrophages from patients with FE was significantly lower (P<0.01), while CD163 was significantly increased (P<0.01). Meanwhile, HIF-1α and Cav-1 mRNA levels were also significantly increased (P<0.01). The age, sputum bacteria positive rate, CAT score, and mMRC score between the patients of FE and iFE with CB phenotype were significantly different (P<0.05). Compared with iFE patients, the fluorescence intensity of CCL3 in sputum macrophages from FE patients was slightly decreased (P<0.05), while CD163 was significantly raised (P<0.01). Meanwhile, HIF-1α and Cav-1 mRNA levels were also significantly increased (P<0.01). The age, duration of disease, FEV1/FVC, sputum bacteria positive rate, CAT score, and mMRC score between the patients of FE and iFE with EM phenotype were significantly different (P<0.05). Compared with iFE patients, the fluorescence intensity of CCL3 in sputum macrophages from FE patients was slightly decreased (P>0.05), while CD163 was slightly raised (P>0.05). Meanwhile, HIF-1α levels were slightly elevated (P>0.05), while Cav-1 expression was significantly increased (P<0.01). There were no significant differences in all clinical features between FE and iFE patients with ACO phenotype. The fluorescence intensity of CCL3 in sputum macrophages from patients with FE was significantly lower than that in iFE patients (P<0.01); there was no significant difference in CD163 (P>0.05). At the same time, the expression of HIF-1α (P<0.01) and Cav-1(P<0.05) also increased significantly. There was a significant negative correlation between CCL3 and HIF-1α or Cav-1 in all FE and FE patients with CB phenotype. CD163 was only positively correlated with HIF-1α in those patients and FE patients with EM phenotype. There was a significant negative correlation between CCL3 and HIF-1α in FE patients with ACO phenotype, while CD163 was significantly positively correlated with HIF-1α.
 Conclusion: The clinical features of FE or iFE patients with CB, EM or ACO phenotype are different, and M2 in induced sputum from FE patients are dominant. HIF-1α may play a key role in the polarization process.


Assuntos
Pulmão , Doença Pulmonar Obstrutiva Crônica , Progressão da Doença , Volume Expiratório Forçado , Humanos , Macrófagos , Fenótipo , Escarro , Capacidade Vital
6.
Nanomaterials (Basel) ; 7(11)2017 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-29140283

RESUMO

A sphere phase liquid crystal (SPLC) composed of three-dimensional twist structures with disclinations among them exists between isotropic phase and blue phase in a very narrow temperature range, about several degrees centigrade. A low concentration polymer template is applied to improve the thermal stability of SPLCs and broadens the temperature range to more than 448 K. By template processing, a wavelength tunable random lasing is demonstrated with dye doped SPLC. With different polymer concentrations, the reconstructed SPLC random lasing may achieve more than 40 nm wavelength continuous shifting by electric field modulation.

7.
Int J Chron Obstruct Pulmon Dis ; 12: 2069-2074, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28790809

RESUMO

PURPOSE: Chronic bronchitis (CB), emphysematous (EM) and asthma-chronic obstructive pulmonary disease (COPD) overlap syndrome (ACOS) phenotypes in COPD are well recognized. This study aimed to investigate distinguishing characteristics of these phenotypes in COPD patients with frequent exacerbations (FE). PATIENTS AND METHODS: A retrospective study was carried out. COPD patients with acute exacerbations were consecutively reviewed from November 2015 to October 2016. Patients were divided into FE and infrequent exacerbations (iFE) subgroups. RESULTS: A total of 142 eligible COPD subjects were reviewed. In the CB phenotype subgroup, age, body mass index, forced expiratory volume in 1 second (FEV1) % predicted, COPD assessment test (CAT), modified Medical Research Council breathlessness measurement (mMRC) dyspnea scale, emphysema scores and arterial carbon dioxide pressure (PaCO2) were significantly different in subjects with FE when compared to those in subjects with iFE of CB. In the EM phenotype subgroup, age, CAT, mMRC scores and history of COPD were different in subjects with FE when compared to those in CB subjects with iFE. Multivariate analysis indicated that FEV1% predicted (odds ratio [OR] =0.90, P=0.04) and PaCO2 (OR =1.22, P=0.02) were independent risk factors for FE in COPD with CB phenotype, and CAT (OR =2.601, P=0.001) was the independent risk factor for FE in COPD with EM phenotype. No significant differences in characteristics were observed in ACOS phenotype subgroups with FE or iFE. CONCLUSION: In CB or EM phenotypes, COPD patients with FE present several differential clinical characteristics compared to patients with iFE, while the characteristics of ACOS phenotype in patients with FE need more investigation.


Assuntos
Asma/fisiopatologia , Bronquite Crônica/fisiopatologia , Pulmão/fisiopatologia , Doença Pulmonar Obstrutiva Crônica/fisiopatologia , Enfisema Pulmonar/fisiopatologia , Idoso , Idoso de 80 Anos ou mais , Asma/classificação , Asma/diagnóstico , Bronquite Crônica/classificação , Bronquite Crônica/diagnóstico , Distribuição de Qui-Quadrado , Progressão da Doença , Dispneia/classificação , Dispneia/diagnóstico , Dispneia/fisiopatologia , Feminino , Volume Expiratório Forçado , Humanos , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Razão de Chances , Fenótipo , Doença Pulmonar Obstrutiva Crônica/classificação , Doença Pulmonar Obstrutiva Crônica/diagnóstico , Enfisema Pulmonar/classificação , Enfisema Pulmonar/diagnóstico , Estudos Retrospectivos , Síndrome
8.
PLoS One ; 11(5): e0155452, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27152626

RESUMO

[This corrects the article DOI: 10.1371/journal.pone.0151768.].

9.
PLoS One ; 11(3): e0151768, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27010560

RESUMO

Kentucky bluegrass (Poa pratensis L.) is a major cool-season turfgrass requiring frequent mowing. Utilization of cultivars with slow growth is a promising method to decrease mowing frequency. In this study, two dwarf mutant selections of Kentucky bluegrass (A12 and A16) induced by space mutation were analyzed for the differentially expressed genes compared with the wild type (WT) by the high-throughput RNA-Seq technology. 253,909 unigenes were obtained by de novo assembly. 24.20% of the unigenes had a significant level of amino acid sequence identity to Brachypodium distachyon proteins, followed by Hordeum vulgare with 18.72% among the non-redundant (NR) Blastx top hits. Assembled unigenes were associated with 32 pathways using KEGG orthology terms and their respective KEGG maps. Between WT and A16 libraries, 4,203 differentially expressed genes (DEGs) were identified, whereas there were 883 DEGs between WT and A12 libraries. Further investigation revealed that the DEG pathways were mainly involved in terpenoid biosynthesis and plant hormone metabolism, which might account for the differences of plant height and leaf blade color between dwarf mutant and WT plants. Our study presents the first comprehensive transcriptomic data and gene function analysis of Poa pratensis L., providing a valuable resource for future studies in plant dwarfing breeding and comparative genome analysis for Pooideae plants.


Assuntos
Meio Ambiente Extraterreno , Mutação , Poa/genética , Sementes/genética , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Ontologia Genética , Genes de Plantas , Kentucky , Mutagênese , Poa/crescimento & desenvolvimento , Sementes/crescimento & desenvolvimento
10.
Med Sci Monit ; 22: 115-20, 2016 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-26752006

RESUMO

BACKGROUND: Triple-negative breast cancer (TNBC) has negative expression of progesterone receptor (PR) and estrogen receptor (ER), and low expression of human epithelial growth factor receptor-2 (HER-2). This study aimed to investigate the expressional profile of cytokines in TNBC patients with significant expression of macrophages. MATERIAL/METHODS: Immunohistochemical (IHC) S-P staining method was used to detect the tumor-associated macrophages (TAMs) marker CD68 expression in 48 cases of TNBC samples. The correlation between CD68 expression and prognosis was analyzed. Expressions of key cytokines--interleukin-6 (IL-6), IL-10, IL-12, IL-1ß, chemokine (C-C motif) ligand-5 (CCL-5), and macrophage inflammatory protein-2 (MIP-2)--were quantified by RT-PCR and enzyme-linked immunosorbent assay (ELISA). RESULTS: Thirty-four out of 48 TNBC samples (71.4%) had CD68-positive expression. IL-6 and CCL-5 were up-regulated in high-infiltrated tumors when compared to low-infiltrated samples. Other cytokines had no significant difference regarding the expression level across groups. CONCLUSIONS: TAMs were up-regulated in most TNBC patients after the surgery. Its expression suggested unfavorable prognosis, especially in the high-infiltrated group. Those tumors with more macrophage also had elevated expression of cytokine IL-6 and chemotactic factor CCL-5, both of which have potency to be clinical index and drug target for TNBC.


Assuntos
Citocinas/metabolismo , Regulação Neoplásica da Expressão Gênica , Macrófagos/citologia , Neoplasias de Mama Triplo Negativas/metabolismo , Adulto , Antígenos CD/metabolismo , Antígenos de Diferenciação Mielomonocítica/metabolismo , Quimiocina CCL5/metabolismo , Quimiocina CXCL2/metabolismo , Ensaio de Imunoadsorção Enzimática , Feminino , Perfilação da Expressão Gênica , Humanos , Imuno-Histoquímica , Interleucina-6/metabolismo , Interleucinas/metabolismo , Pessoa de Meia-Idade
11.
ACS Appl Mater Interfaces ; 6(3): 1380-4, 2014 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-24409988

RESUMO

A phototunable laminated liquid crystal film with simultaneous multicolor reflection is successfully fabricated by doping a light-driven chiral molecular switch into the laminated cholesteric liquid crystal structure. Upon UV-light irradiation, the reflection notches and their hyper-reflectivity properties can be precisely tuned, and their original state can be properly returned by visible-light irradiation.

12.
Chem Commun (Camb) ; 50(6): 691-4, 2014 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-24286108

RESUMO

A chiral nematic liquid crystal-photopolymerizable monomer-chiral azobenzene compound composite was prepared and then polymerized under UV irradiation. The reflection wavelength of the composite can be extended to cover the 1000-2400 nm range and also be adjusted to the visible light region by controlling the concentration of chiral compounds.

13.
Chem Commun (Camb) ; 49(86): 10097-9, 2013 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-24045847

RESUMO

A new series of azobenzene-dimers were synthesized and doped into the blue phase liquid crystals to broaden the temperature range of BPs. It is found that not only can the reflection wavelength of BPI be reversibly controlled but BPI can also be transformed into the cholesteric phase owing to isomerization of azobenzene induced by light.

14.
Nonlinear Anal Real World Appl ; 12(1): 658-670, 2011 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-21057661

RESUMO

In this paper we discuss a model of allelopathy and bacteriocin in the chemostat with a wild-type organism and a single mutant. Dynamical properties of this model show the basic competition between two microorganisms. A qualitative analysis about the boundary equilibrium, a state that microorganisms both vanish, is carried out. The existence and uniqueness of the interior equilibrium are proved by a technical reduction to the singularity of a matrix. Its dynamical properties are given by using the index theory of equilibria. We further discuss its bifurcations. Our results are demonstrated by numerical simulations.

15.
Zhonghua Jie He He Hu Xi Za Zhi ; 30(9): 691-6, 2007 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-18070556

RESUMO

OBJECTIVE: To investigate the effects of injured airway epithelial cells on the transdifferentiation of sub-epithelial fibroblasts, thus addressing the mechanisms of characteristic airway remodeling in asthmatic airway. METHODS: Human sub-epithelial fibroblasts (SEF) or SEF in collagen gels were co-cultured with culture dishes which containing or not containing bronchial epithelial cells (16HBE) for 4 days. The experiments were divided into 7 groups according to different treatment to 16HBE; Group A: culture dishes not containing 16HBE; Group B: culture dishes containing normal 16HBE; Group C: culture dishes containing 16HBE treated with mechanical scrape; Group D: culture dishes containing 16HBE treated with mechanical scrape plus LPS stimulation; Group E: culture dishes containing 16HBE pre-added with endothelin receptor A inhibitor (BQ123) followed by treatment with mechanical scrape plus LPS stimulation; Group F: culture dishes containing 16HBE pre-added with TGF-beta(1) neutralizing antibody followed by treatment with mechanical scrape plus LPS stimulation; Group G: culture dishes containing 16HBE pre-added with BQ123 plus TGF-beta(1) neutralizing antibody followed by treatment with mechanical scrape plus LPS stimulation. The expression of alpha-smooth muscle actin (alpha-SMA) in the SEF was assessed by immunofluorescence or Western blotting. The diameters of the collagen gels containing SEF (8 samples in each group) were measured. Moreover, SEF in group A, B or D were selected, and the dynamic changes of intracellular calcium concentration [Ca(2+)]i were observed (at least 6 cells were analyzed in each group) by laser co-focal microscopy in the transdifferentiated SEF in response to the stimulation of the supernatant of 16HBE treated with mechanical scrape plus LPS stimulation for 24 h. RESULTS: alpha-SMA expression in SEF was most abundant in group D compared with other groups. Relative contraction percentage was also significantly higher in group D [(53 +/- 8)%] than that in group B [(10 +/- 10)%], group C [(24 +/- 8)%], group E [(36 +/- 9)%], group F [(37 +/- 3)%] or group G [(31 +/- 7)%], respectively (F = 24.80, 38.10, all P < 0.01). Increased fluorescence intensity of [Ca(2+)]i was observed in the SEF stimulated with the supernatant of the injured 16HBE in all groups, which was significant in group D (F = 16.60, 8.97; all P < 0.01). CONCLUSIONS: Injured airway epithelial cells induced the transdifferentiation of SEF towards alpha-SMA-expressing and elevated contractile myofibroblasts, in which both ET-1 and TGF-beta(1) may play crucial roles. The supernatant of injured airway epithelial cells-induced influx of [Ca(2+)]i may be an early signal for initiating the increased contractility in the transdifferentiated SEF.


Assuntos
Diferenciação Celular , Células Epiteliais/patologia , Fibroblastos/patologia , Mucosa Respiratória/patologia , Transdiferenciação Celular , Células Cultivadas , Células Epiteliais/citologia , Epitélio/metabolismo , Fibroblastos/citologia , Humanos , Fator de Crescimento Transformador beta1/metabolismo
16.
Zhonghua Jie He He Hu Xi Za Zhi ; 28(10): 698-703, 2005 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-16255956

RESUMO

OBJECTIVE: To address the possible role of injured airway epithelium in initiating transdifferentiation of sub-epithelial fibroblasts into myofibroblasts and accelerating cell proliferation in sub-epithelial fibroblasts, which may be involved in airway hyperresponsiveness in asthma. METHODS: Human primary cultured sub-epithelial fibroblasts were co-cultured with human bronchial epithelial cells (16HBE) which were treated with lipopolysaccharide (LPS) plus mechanical scratch prior to co-culture. The procedure was also performed in the presence or absence of endothelin (ET) receptor A inhibitor (BQ123), transforming growth factor-beta(1) (TGF-beta(1)) neutralized antibody, respectively or simultaneously, followed by immunostaining, Western blotting and bromodeoxyuridine (BrdU) incorporation respectively to detect alpha-SMA expression and cell proliferation in the co-cultured sub-epithelial fibroblasts. Using the inhibitors specific for mitogen-activated protein kinases (MAPKs) pathways, the role of MAPKs pathways in activating the expression of alpha-SMA was evaluated. In addition, the interaction between matrix metalloproteinases (MMPs) and ET-1 was investigated by cell transfection with anti-ET-1 converting enzyme (anti-ECE) mRNA expression plasmid followed by gelatin zymography analysis. RESULTS: 16HBE treated with LPS plus mechanical injury induced alpha-SMA expression in sub-epithelial fibroblasts and accelerated BrdU incorporation in the cells. BQ123, TGF-beta(1) neutralized antibody, specific inhibitors for p38 MAPK and extracellular signal-regulated kinase 1/2 (ERK1/2) were able to block the induction respectively to a certain extent. Phosphorylated p38 MAPK and ERK1/2 were detected in the sub-epithelial fibroblasts 10 min after being co-cultured with injured 16HBE. Compared to normal control (16HBE transfected with pEGFPN(2)) or those cells transfected with anti-ECE mRNA expression plasmids, ET-1 released from the 16HBE cells transfected with pEGFPN(2) into supernatants were increased significantly after the treatment described as above: 16HBE pre-transfected with pEGFP-N(2) expression plasmid before being treated with mechanical scrape plus LPS stimulation: (15.00 +/- 0.86) pg/ml; 16HBE pre-transfected with anti-ECE expression plasmid before being treated with mechanical scrape plus LPS stimulation: (7.57 +/- 0.94) pg/ml (all P < 0.01). At the same time, the activities of MMP-2 and MMP-9 were enhanced. CONCLUSIONS: Injured airway epithelial cells induced the transdifferentiation of sub-epithelial fibroblasts into myofibroblasts, which may be mediated by ET-1 and TGF-beta(1) through MARKs pathways such as p38 MAPK and ERK 1/2.


Assuntos
Asma/patologia , Diferenciação Celular , Fibroblastos/citologia , Mucosa Respiratória/patologia , Actinas/metabolismo , Asma/metabolismo , Linhagem Celular Transformada , Proliferação de Células , Transdiferenciação Celular , Técnicas de Cocultura , Endotelina-1/metabolismo , Células Epiteliais/citologia , Células Epiteliais/metabolismo , Humanos , Lipopolissacarídeos/farmacologia , Proteína Quinase 3 Ativada por Mitógeno/antagonistas & inibidores , Proteína Quinase 3 Ativada por Mitógeno/metabolismo , Peptídeos Cíclicos/farmacologia , Fosforilação , Inibidores de Proteínas Quinases/farmacologia , Mucosa Respiratória/metabolismo , Transfecção , Fator de Crescimento Transformador beta1/antagonistas & inibidores , Fator de Crescimento Transformador beta1/metabolismo , Proteínas Quinases p38 Ativadas por Mitógeno/antagonistas & inibidores , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
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