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1.
J Gene Med ; : e3139, 2019 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-31697008

RESUMO

BACKGROUND: The aim of this study was to investigate the association of several single nucleotide polymorphisms (SNPs) within Toll-like receptors 4 (TLR4) gene and additional gene- environment interaction with primary open angle glaucoma (POAG) risk. METHODS: Testing for Hardy-Weinberg equilibrium in controls and haplotype analysis are performed using SNPstats. Generalized multifactor dimensionality reduction (GMDR) was performed to test the interaction effects among 4 SNPs within TLR4 gene and environmental factors. Logistic regression was performed to calculate the ORs (95%CI) for association between 4 SNPs within TLR4 gene and POAG risk. RESULTS: The POAG risk was significantly higher in carriers with the T allele of rs4986791, the T allele of rs2149356 within TLR4 gene than those with wild genotype, adjusted ORs (95%CI) were 1.65 (1.23-2.12) and 1.70 (1.16-2.31). GMDR model suggested a significant two-locus model (p=0.0010) involving rs2149356 and alcohol drinking. Alcohol drinkers with rs2149356- GT+ TT genotype within TLR4 gene have the highest POAG risk, compared to never alcohol drinkers with rs2149356- GG genotype, OR (95%CI) = 2.62 (1.48-3.78), after covariates adjustment. But the study did not find a significant any-locus model involving SNP and smoking. In all samples, the haplotype rs2149356 - G- rs7873784- C was observed most frequently in two groups, with 47.47% and 48.21% for the POAG patients and controls, respectively. The results also indicated no significant haplotype associated with POAG risk. CONCLUSIONS: The minor alleles of rs4986791 and rs2149356 within TLR4 gene, interaction between rs2149356 and alcohol drinking were all associated with increased POAG risk.

2.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 36(11): 1085-1089, 2019 Nov 10.
Artigo em Chinês | MEDLINE | ID: mdl-31703131

RESUMO

OBJECTIVE: To delineate the clinical and genetic features of two pedigrees affected with aromatic L-amino acid decarboxylase (AADC) deficiency. METHODS: The clinical features, family history and results of genetic testing of 2 patients with AADC deficiency were retrospectively analyzed. RESULTS: Both patients featured hypotension, developmental delay and oculogyric crisis during infancy. Genetic testing confirmed that they have respectively carried c.714+ 4 (IVS6) A to T/c.175(exon2)G TO A compound heterozygous variants and c.714+ 4(IVS6)A to T homozygous variant. CONCLUSION: The clinical manifestation of children with AADC deficiency may include hypotonia, developmental delay and paroxysmal oculogyric crisis. The combination of 3-O-methyldopa testing and variant analysis is not only very useful for early diagnosis, but also important for the evaluation of treatment effect and prognosis of the disease. Discovery of the novel variants has enriched the variant spectrum of AADC deficiency.

3.
Artif Cells Nanomed Biotechnol ; 47(1): 3873-3884, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31566012

RESUMO

Cell proliferation constitutes the fundamental process and driving force behind regrowth during liver regeneration (LR). However, it remains unclear how competing endogenous RNA (ceRNA) networks affect hepatocyte proliferation and liver regeneration. Therefore, this study was designed to explore an LR-specific ceRNA network, which regulates cell proliferation. Based on the microarray data of mRNAs, and high-throughput sequencing data of miRNAs and circRNAs from regenerating livers, this study initially applied known 1484 LR associated mRNAs to perform GO analysis, and then selected 169 LR associated mRNAs involved in cell proliferation and the cell cycle. Subsequently, 188 interactive miRNA-mRNA pairs and 5206 circRNA-miRNA pairs, respectively, were predicted using bioinformatics methods. Next, in view of the differential expressions of these ceRNAs during LR, 26 miRNA-mRNA pairs and 71 circRNA-miRNA pairs were applied to generate a circRNA-miRNA-mRNA regulatory network, and only 14 triple interactive groups were obtained based on the predicted inverse interactions among ceRNAs. Finally, circ_19698/miR-423-5p axis was demonstrated to promote cell proliferation by modulating the expression of MYC, CCNA2, and CCND1 in rat BRL-3A cells. This study suggests a potential regulatory mechanism of cell proliferation in regenerating livers, as well as a novel pathway for modulating ceRNA networks to promote liver regeneration.

4.
Medicine (Baltimore) ; 98(40): e17393, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31577747

RESUMO

RATIONALE: Bacteremia caused by polymicrobial infections are rare but dangerous. We report a case of hepatic abscess combined with polymicrobial bacteremia in a 49-year-old male patient after surgery and transcatheter arterial chemoembolization (TACE). PATIENT CONCERNS: The patient was admitted to hospital with metastatic liver cancer for periodic chemotherapy and developed a high fever and tenderness to the liver following surgery and TACE. DIAGNOSIS: Hepatic abscess combined with polymicrobial bacteremia. INTERVENTIONS: The clinician formulated a therapy in accordance with the drug susceptibility test and the empirical drug use for anaerobic bacteria. A comprehensive treatment plan was adopted, on the basis of the combination of nitrazole and imipenem as anti-infection drugs as well as continuous abscess drainage. OUTCOMES: After comprehensive therapy, the patient was ultimately discharged without any residual symptoms. LESSONS: Bloodstream infection caused by multiple bacteria increases the difficulty of anti-infection treatments, leading to poor treatment outcome and high mortality. Therefore, a fast and accurate diagnosis of polymicrobial bacteremia is key for initiation of an effective antimicrobial treatment. Additionally, pre-operative prophylactic antibiotics are advisable when patients have a history of abdominal surgery and are immune-compromised.


Assuntos
Bacteriemia/etiologia , Carcinoma Hepatocelular/terapia , Quimioembolização Terapêutica/efeitos adversos , Abscesso Hepático/etiologia , Neoplasias Hepáticas/terapia , Antibacterianos/uso terapêutico , Bacteriemia/tratamento farmacológico , Bacteriemia/microbiologia , Carcinoma Hepatocelular/cirurgia , Coinfecção , Drenagem , Humanos , Abscesso Hepático/tratamento farmacológico , Abscesso Hepático/microbiologia , Neoplasias Hepáticas/cirurgia , Masculino , Pessoa de Meia-Idade , Metástase Neoplásica
5.
Org Lett ; 21(21): 8603-8606, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31613638

RESUMO

We report a highly efficient copper-catalyzed three-component reaction of alkylamines, acetylenedicarboxylates, and α-bromocarbonyls for the assembly of fully substituted 1,3-dihydro-2H-pyrrol-2-ones. A variety of alkylamines and ammonium salt are functionalized with acetylenedicarboxylates and α-bromocarbonyls. N-aryl enaminoesters are also successfully alkylated with α-bromocarbonyls. This protocol is understood to proceed through radical Heck-type coupling of in-situ-generated bulky trisubstituted alkenes with bulky tertiary alkyl bromides, which is realized for the first time.

6.
Org Lett ; 21(19): 7795-7798, 2019 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-31524415

RESUMO

A utility approach toward trisubstituted allenes through Pd-catalyzed alkynyl Heck coupling reaction of alkynes and aryl iodides is reported. This process proceeded via regioselective carbopalladation of 1-aryl-1-alkynes to give alkenyl palladium species, which undergo ß-hydride elimination to provide 1,1-diarylallenes in 25-71% yields. This method features unique regioselectivity and high functional group compatibility.

8.
Sci Rep ; 9(1): 13139, 2019 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-31511564

RESUMO

Escherichia coli is an important pathogen of nosocomial infection in clinical research, Thus, exploring new methods for the rapid detection of this pathogen is urgent. We reported the early release of molecular volatile indole vapour of E. coli cultures and blood cultures analyzed by direct atmospheric corona discharge ionization mass spectrometry (CDI-MS). The concentration of indole in E. coli cultures remarkably increases during the early log and lag phases of bacterial growth, thereby enabling early detection. Technical replicates were cultivated for 3 days for reference diagnosis using current conventional bacteraemia detection. A reference MS screen of common microbes from other genera confirmed that the peaks at m/z 116 signal corresponded to indole were specifically present in E. coli. Our results indicated that volatile indole based on CDI-MS without the need for any sample pretreatment is highly suitable for the reliable and cost-efficient differentiation of E. coli, especially for bacteraemia in humans.

9.
Chemosphere ; 235: 1172-1179, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31561308

RESUMO

Rice (Oryza sativa L.) was cultivated in a Cd-contaminated soils with rice straw biochar (BC) and water-washed rice straw biochar (W-BC) were applied to investigate the underlying mechanisms and possible reasons for biochar's weakening effects on the immobilization of Cd in soil-rice system. The results indicated that W-BC reduced the Cd concentration in pore water as well as in the roots and shoots of rice by 26.24%, 53.23% and 62.47% respectively. On the contrary, there was an increase in Cd contents by 50.27% in pore water, 2.32% in the roots, and 12.80% in the shoots of rice under BC treatment. Furthermore, Cd content in rice shoot was significantly and positively correlated with Cl- addition to the soil (P < 0.01). This phenomenon could be attributed to several combined effects: (1) the increase of Cl- in the soil decreased the soil pH, enhanced the dissolved organic carbon in soil pore water and increased the complexes of Cd2+ and Cl-, resulting in the release of Cd from solid phase into solution phase, (2) the chloride in the soil increased the uptake of CdCl+ instead of Cd2+ by the roots, thereby causing an increase of Cd in rice tissues. These results demonstrate for the first time that biochar with high chloride content could weaken its immobilization effects on soil Cd and even enhance Cd uptake by rice.


Assuntos
Cádmio/farmacocinética , Carvão Vegetal/farmacologia , Cloro/farmacologia , Oryza/metabolismo , Solo/química , Carvão Vegetal/química , Poluição Ambiental , Poluentes do Solo/análise , Distribuição Tecidual
10.
ACS Sens ; 4(9): 2546-2552, 2019 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-31456397

RESUMO

SnSe2 is an anisotropic binary-layered material with rich physics, which could see it used for a variety of potential applications. Here, we investigate the gas-sensing properties of SnSe2 using first-principles calculations and verify predictions using a gas sensor made of few-layer SnSe2 grown by chemical vapor deposition. Theoretical simulations indicate that electrons transfer from SnSe2 to NO2, whereas the direction of charge transfer is the opposite for NH3. Notably, a flat molecular band appears around the Fermi energy after NO2 adsorption and the induced molecular band is close to the conduction band minimum. Moreover, compared with NH3, NO2 molecules adsorbed on SnSe2 have a lower adsorption energy and a higher charge transfer value. The dynamic-sensing responses of SnSe2 sensors confirm the theoretical predictions. The good match between the theoretical prediction and experimental demonstration suggests that the underlying sensing mechanism is related to the charge transfer and induced flat band. Our results provide a guideline for designing high-performance gas sensors based on SnSe2.

11.
Org Lett ; 21(16): 6366-6369, 2019 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-31361496

RESUMO

A rhodium(III)-catalyzed C-H acylmethylation of tridentate [2,2'-bipyridine]-6-carboxamides was developed. A variety of [2,2'-bipyridine]-6-carboxamides could be monoalkylated exclusively at the C3 position with sulfoxonium ylides as carbene precursors, giving 3-alkylated products in high yields. This protocol proceeds through a rollover cyclometalation pathway, has a broad range scope of substrates, and exhibits excellent functional group tolerance.

12.
J Hazard Mater ; 379: 120701, 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31299630

RESUMO

The influence of elemental sulfur (S(0)) amendment on methylmercury (MeHg) accumulation in rice and the chemical form of Hg in the rhizosphere were investigated under waterlogged conditions in Hg-contaminated soil (the majority of the Hg (˜70%) in forms similar to HgS). Different levels of S(0) addition increased the MeHg accumulation in rice. After a sequential extraction analysis of the chemical forms of Hg in the rhizosphere, the results showed that S(0) addition increased the organic bound Hg and decreased the residual Hg in the soils. An Hg LIII XANES further showed that S(0) addition increased the proportion of Hg in the form of RS-Hg-SR and decreased the proportion of Hg in the form of HgS, indicating that S(0) input may reactivate the non-bioavailable Hg in the rhizosphere and improve the net Hg methylation. These findings suggest that the application of S fertilizers to Hg-contaminated paddy soils may increase the MeHg concentration in the edible parts of crops, which may lead to more potential health problems in humans depending on the crop type. However, our study also suggests that S(0) addition could be an effective measure for mobilizing the insoluble Hg and accelerating the phytoremediation process in Hg-contaminated paddy soils.

13.
Artif Cells Nanomed Biotechnol ; 47(1): 2957-2966, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31315467

RESUMO

Researchers have been exploring the genetic mechanisms underlying the control of liver regeneration (LR). However, an integrated analysis of circRNAs expression of rat regenerating livers during the proliferation phase has not been performed yet. For this purpose, circRNAs expression profile was globally analyzed by high-throughput sequencing. It showed that 10,003 circRNAs were detected, and 164 circRNAs were differentially expressed. Subsequently, 27 circRNAs were predicted to bind to 58 candidate miRNAs and compete for miRNA-binding sites with 2195 mRNAs. By applying GO and KEGG analysis, it was predicted that these circRNAs significantly participated in tissue regeneration, regulation of cell proliferation and Ras, p53, Wnt, Jak-STAT, MAPK signalling pathways. Based on the number of the corresponding miRNAs and their role enriched and reported in cell proliferation of LR or hepatocellular carcinoma, four kinds of circRNAs (circ_03848, circ_08236, circ_13398 and circ_15013) were considered as the key circRNAs. The predicted competing endogenous RNA networks and bioinformatics analysis revealed the potential role of these circRNAs in LR, which would provide useful information for understanding the mechanism of LR.

14.
Int J Mol Sci ; 20(10)2019 May 19.
Artigo em Inglês | MEDLINE | ID: mdl-31109136

RESUMO

The morphological development of the leaf greatly influences plant architecture and crop yields. The maize leaf is composed of a leaf blade, ligule and sheath. Although extensive transcriptional profiling of the tissues along the longitudinal axis of the developing maize leaf blade has been conducted, little is known about the transcriptional dynamics in sheath tissues, which play important roles in supporting the leaf blade. Using a comprehensive transcriptome dataset, we demonstrated that the leaf sheath transcriptome dynamically changes during maturation, with the construction of basic cellular structures at the earliest stages of sheath maturation with a transition to cell wall biosynthesis and modifications. The transcriptome again changes with photosynthesis and lignin biosynthesis at the last stage of sheath tissue maturation. The different tissues of the maize leaf are highly specialized in their biological functions and we identified 15 genes expressed at significantly higher levels in the leaf sheath compared with their expression in the leaf blade, including the BOP2 homologs GRMZM2G026556 and GRMZM2G022606, DOGT1 (GRMZM2G403740) and transcription factors from the B3 domain, C2H2 zinc finger and homeobox gene families, implicating these genes in sheath maturation and organ specialization.


Assuntos
Regulação da Expressão Gênica de Plantas , Folhas de Planta/genética , Zea mays/genética , Perfilação da Expressão Gênica , Regulação da Expressão Gênica no Desenvolvimento , Genes de Plantas , Folhas de Planta/crescimento & desenvolvimento , Proteínas de Plantas/genética , Transcriptoma , Zea mays/crescimento & desenvolvimento
15.
J Exp Clin Cancer Res ; 38(1): 193, 2019 May 14.
Artigo em Inglês | MEDLINE | ID: mdl-31088500

RESUMO

BACKGROUND: Several targeted immunotherapies have recently showed significant advances in treatment of non-small cell lung cancer (NSCLC), including antibodies and inhibitors targeting programmed death-1 (PD-1) and its ligand (PD-L1). METHODS: Tumor tissue samples were prospectively collected from 183 patients with NSCLC including lung adenocarcinoma (ADC) and squamous cell carcinoma (SQCC). PD-L1 expression level was measured by immunohistochemistry assay and tumor mutational burden (TMB) status was assessed by next generation sequencing. Correlations between PD-L1 expressions, TMB status with clinicopathological characteristics were analyzed. RESULTS: PD-L1 expression was detected in 37% of ADC group and 55% in SQCC group while all clinicopathological characteristics were found comparable between these two groups. PD-L1 expression was negatively associated with overall survival in ADC group (P < 0.0001) but not in SQCC group (P = 0.418). In consistent with PD-L1 expression level, TMB status was significantly lower in ADC subjects as compared to SQCC subjects (P = 0.024) while PD-L1 positive subgroup and TMB high subgroup shared less subjects within ADC group than SQCC group. More importantly, the combination of TMB status and PD-L1 expression successfully identified responders, who showed significant longer median overall survival than non-responders (32 months vs. 8.5 months) in ADC subjects (P < 0.0001) but not in SQCC subjects. CONCLUSIONS: Here we tested the hypothesis that monitoring TMB, in addition to the existing PD-L1 expression level, could represent valuable non-invasive biomarkers for the chemotherapy and targeted therapy. Further analyses are in need to further assess the prognostic value of TMB for ADC and SQCC patients receiving immunotherapy.

16.
Nat Commun ; 10(1): 2217, 2019 05 17.
Artigo em Inglês | MEDLINE | ID: mdl-31101813

RESUMO

Cd3As2 is a three-dimensional Dirac semimetal with separated Dirac points in momentum space. In spite of extensive transport and spectroscopic studies on its exotic properties, the evidence of superconductivity in its surface states remains elusive. Here, we report the observation of proximity-induced surface superconductivity in Nb/Cd3As2 hybrid structures. Our four-terminal transport measurement identifies a pronounced proximity-induced pairing gap (gap size comparable to Nb) on the surfaces, which exhibits a flat conductance plateau in differential conductance spectra, consistent with our theoretical simulations. The surface supercurrent from Nb/Cd3As2/Nb junctions is also achieved with a Fraunhofer/SQUID-like pattern under out-of-plane/in-plane magnetic fields, respectively. The resultant mapping shows a predominant distribution on the top and bottom surfaces as the bulk carriers are depleted, which can be regarded as a higher dimensional analog of edge supercurrent in two-dimensional quantum spin Hall insulators. Our study provides the evidence of surface superconductivity in Dirac semimetals.

17.
Zhongguo Dang Dai Er Ke Za Zhi ; 21(5): 450-453, 2019 May.
Artigo em Chinês | MEDLINE | ID: mdl-31104661

RESUMO

OBJECTIVE: To study the effects of ketogenic diet (KD) on lipid metabolism in children with intractable epilepsy and the risk of atherosclerosis in children treated with KD assessed by changes in lipid profile. METHODS: The clinical data of 47 children with intractable epilepsy from 2013 to 2017 were collected. Blood lipid levels including triglycerides (TG), total cholesterol (TC), high-density lipoprotein (HDL) and low-density lipoprotein (LDL), were detected before and 3 months after KD treatment. LDL/HDL ratio, arterial stiffness index (AI), atherogenic index of plasma (AIP) and lipid comprehensive index (LCI) were calculated to assess the risk of atherosclerosis. RESULTS: After 3 months of KD treatment, the TG and TC levels were slightly higher than those before treatment, and the HDL levels were slightly lower than those before treatment, but the differences were not statistically significant (P>0.05). The LDL levels of the children after 3 months of KD treatment were significantly higher than those before treatment (P<0.05). After 3 months of KD treatment, the LDL/HDL ratio and AI, AIP and LCI levels of the children were increased compared with those before treatment, but only the increase of the LDL/HDL ratio was statistically significant (P<0.05). CONCLUSIONS: KD treatment may lead to increase in LDL level and LDL/HDL ratio, suggesting that KD treatment may increase the risk of atherosclerosis.


Assuntos
Dieta Cetogênica , Epilepsia Resistente a Medicamentos , Criança , Humanos , Metabolismo dos Lipídeos , Lipídeos , Triglicerídeos
18.
J Med Microbiol ; 68(6): 882-889, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31050634

RESUMO

PURPOSE: The aim of the present study was to investigate the dissemination of carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates in integrated intensive care units (IICUs) and emergency ICUs (EICUs) for controlling the spread of CRKP in different ICUs of the hospital. METHODOLOGY: From January 2016 to April 2017, a total of 46 non-duplicate CRKP isolates were consecutively isolated from a tertiary hospital. The production of carbapenemases was determined by the modified carbapenem inactivation method (mCIM) test. The resistance and virulence-associated genes were detected by PCR and DNA sequencing. A hypermucoviscosity phenotype was identified by the string test. Bacterial clonal relatedness of the CRKP isolates tested was determined by multi-locus sequence typing (MLST) and PFGE. RESULTS: All CRKP isolates showed multiple drug resistance. All CRKP isolates harboured blaKPC-2-encoding carbapenemase and at least one of the other ß-lactamase genes tested, with positive rates of 89.1  % (41/46) for blaCTX-M-65. qnrS was found among 76.1  % (35/46) of the CRKP isolates. A hypermucoviscosity phenotype was found in only two (4.3 %, 2/46) CRKP isolates. The virulence-associated genes with positive rates of more than 90  % among the 46 isolates tested included wabG (100 %, 46/46), ycf (100 %, 46/46), ureA (95.6 %, 44/46) and fim H (95.6 %, 44/46). MLST results showed that 46 CRKP isolates belonged to ST11 (95.6 %, 44/46) and ST86 (4.4 %, 2/46). PFGE patterns showed four clusters. CONCLUSION: The CRKP ST11 clone with co-production of CTX-M-65 and KPC-2 disseminated in ICUs of this tertiary teaching hospital in central China. The emergence of CRKP with a hypermucoviscosity phenotype in ICUs should be of particular concern.


Assuntos
Proteínas de Bactérias/genética , Enterobacteriáceas Resistentes a Carbapenêmicos , Carbapenêmicos/farmacologia , Infecções por Klebsiella/epidemiologia , Klebsiella pneumoniae/enzimologia , beta-Lactamases/genética , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Técnicas de Tipagem Bacteriana , China/epidemiologia , Serviços Médicos de Emergência , Feminino , Humanos , Unidades de Terapia Intensiva , Infecções por Klebsiella/tratamento farmacológico , Infecções por Klebsiella/microbiologia , Klebsiella pneumoniae/efeitos dos fármacos , Klebsiella pneumoniae/genética , Klebsiella pneumoniae/isolamento & purificação , Masculino , Pessoa de Meia-Idade , Tipagem de Sequências Multilocus , Centros de Atenção Terciária , Adulto Jovem
19.
Plant Biotechnol J ; 17(11): 2153-2168, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30972847

RESUMO

Southern corn rust (SCR), which is a destructive disease caused by Puccinia polysora Underw. (P. polysora), commonly occurs in warm-temperate and tropical regions. To identify candidate proteins related to SCR resistance and characterize the molecular mechanisms underlying the maize-P. polysora interaction, a comparative proteomic analysis of susceptible and resistant maize lines was performed. Statistical analyses revealed 1489 differentially abundant proteins in the resistant line, as well as 1035 differentially abundant proteins in the susceptible line. After the P. polysora infection, the abundance of one remorin protein (ZmREM1.3) increased in the resistant genotype, but decreased in the susceptible genotype. Plant-specific remorins are important for responses to microbial infections as well as plant signalling processes. In this study, transgenic maize plants overexpressing ZmREM1.3 exhibited enhanced resistance to the biotrophic P. polysora. In contrast, homozygous ZmREM1.3 UniformMu mutant plants were significantly more susceptible to P. polysora than wild-type plants. Additionally, the ZmREM1.3-overexpressing plants accumulated more salicylic acid (SA) and jasmonic acid (JA). Moreover, the expression levels of defence-related genes were higher in ZmREM1.3-overexpressing maize plants than in non-transgenic control plants in response to the P. polysora infection. Overall, our results provide evidence that ZmREM1.3 positively regulates maize defences against P. polysora likely via SA/JA-mediated defence signalling pathways. This study represents the first large-scale proteomic analysis of the molecular mechanisms underlying the maize-P. polysora interaction. This is also the first report confirming the remorin protein family affects plant resistance to SCR.

20.
Platelets ; 30(7): 828-835, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30810479

RESUMO

Immune thrombocytopenic purpura (ITP) is an autoimmune disorder presenting with low platelet count <100 × 109/L. The condition affects both adults and children. Thrombopoietin receptor agonists (TPO-RAs) are second-line of therapy that includes Romiplostim and Eltrombopag, which stimulate the production of normally functioning platelets. Although the biological effect of these drugs is well established, there has not been a meta-analysis in children. To estimate the efficacy and safety of Romiplostim and Eltrombopag, we performed a systematic review and meta-analysis in children with chronic ITP. Systematic literature search was conducted in the following database: PubMed, EMBASE, and Cochrane Central Register of Controlled Trials (CENTRAL). Review Manager 5.3 for Windows was used to analyze the data. Five randomized controlled trials with total of 261 pediatric patients from 1-17 years of age were included. The efficacy and safety analysis showed TPO-RA groups were superior over placebo, and there was no difference in adverse event occurrence between TPO-RA (Romiplostim and Eltrombopag) and placebo groups. The efficacy and safety of Eltrombopag did not differ significantly from those of Romiplostim. Both drugs were effective in treatment of children with chronic ITP. Our findings extend the currently available data on ITP treatment and is helpful for pediatric health providers and for the design of future clinical trials.

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