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1.
Small ; : e1905938, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-32115895

RESUMO

Nanobactericides represent one of the most efficient and promising strategies for eliminating bacterial infection considering the increasing resistance threats of conventional antibiotics. Black phosphorus (BP) is the most exciting postgraphene layered 2D nanomaterial with convincing physiochemical properties, yet the study of BP-based antibiotics is still in its infancy. Here, a compact silver nanoparticle (AgNP)-doped black phosphorus nanosheet (BPN) is constructed to synergistically enhance solar disinfection through the promoted reactive oxygen species (ROS) photogeneration, which is attributed to the improved electron-hole separation and recombination of BPNs as revealed from the systematic experimental studies. An in-depth density functional theory (DFT) calculation confirms that the integrated AgNPs provide a preferred site for facilitating the adsorption and activation of O2 , thus promoting the more efficient and robust ROS generation on BPN-AgNP nanohybrids. Besides the enhanced photoinduced ROS, the anchored AgNPs simultaneously lead to a dramatically increased affinity toward bacteria, which facilitates a synergetic pathogen inactivation. Significantly, the convincing antimicrobial BPN-AgNP contributes to the prominent wound healing and antimicrobial ability in vivo with minimized biological burden. This sophisticated design of new 2D nanohybrids opens a new avenue for further exploiting BP-based nanohybrids in portable bandage and broad-spectrum disinfection applications.

2.
Antioxidants (Basel) ; 9(4)2020 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-32218152

RESUMO

PURPOSE: Cataracts are a major cause of visual acuity deterioration in diabetes mellitus (DM) in developed and developing countries. Studies have demonstrated that overproduction of AKR1B1 and receptor for advanced glycation end products (RAGE) plays a major role in the pathogenesis of diabetic cataracts, but it is unclear whether the prevalence of diabetic cataracts is related to epithelial-mesenchymal transition (EMT) in lens epithelial cells. This study aimed to analyze the role of EMT in cataract formation of DM patients. METHODS: Immunofluorescence and immunohistochemistry assays were used to estimate AKR1B1, RAGE, AMPK, and EMT levels in epithelial human lens of DM or non-DM cataracts. RESULTS: Immunohistochemical staining demonstrated that pathologic phases and N-cadherin expression levels were significantly higher in epithelial human lens of DM (+) compared to DM (-) cataracts. Immunofluorescent staining showed that AKR1B1 and RAGE were significantly higher in epithelial human lens of DM (+) compared to DM (-) cataracts. Interestingly, acetyl superoxide dismutase 2 (AcSOD2) levels were significantly higher in DM patients' lens epithelial cells (LECs), whereas AMPKT172 phosphorylation was significantly increased in non-DM patients. This indicates that AMPKT172 might be related to superoxide reduction and diabetic cataract formation. CONCLUSIONS: Our results suggest that AKR1B1 overexpression can decrease AMPK activation, thereby increasing AcSOD2 and RAGE-induced EMT in epithelial human lens of DM cataracts. These novel findings suggest that AKR inhibitors may be candidates for the pharmacological prevention of cataracts in patients with DM.

3.
Anal Bioanal Chem ; 2020 Mar 03.
Artigo em Inglês | MEDLINE | ID: mdl-32125466

RESUMO

A label-free fluorescent nanoprobe for iodide ion (I-) detection was developed based on the direct fluorescence quenching of spermine-functionalized carbon dots (SC-dots), whether in complex biological fluids or living cells. The positively charged SC-dots were fabricated via one-step microwave synthesis and exhibited excellent optical properties. Due to the strong quenching ability of I-, SC-dots were utilized for I- detection with high sensitivity and excellent selectivity, which offered a relatively low detection limit of 0.18 µM. This strategy was also successfully applied for I- detections in human serum and HeLa cells. The detection process is facile, highly sensitive and selective, providing a new insight into the potential applications of SC-dots for anion nanoprobe designs in clinical diagnosis and other biologically related areas. Graphical abstract.

4.
J Clin Lab Anal ; : e23295, 2020 Mar 13.
Artigo em Inglês | MEDLINE | ID: mdl-32170805

RESUMO

BACKGROUND: Platelets play a role in tumor cell growth, metastasis, and angiogenesis, and the present study aimed to evaluate diagnostic and prognostic values of platelet parameters in patients with gynecological tumors. METHODS: A total of 1062 women were included. Differences of platelet parameters (platelet count [PLT], plateletcrit [PCT], mean platelet volume [MPV], platelet-large cell rate [P-LCR], and platelet distribution width [PDW]) between different categories were analyzed by nonparametric test. The optimal cutoff value was calculated with receiver operating characteristic analysis. Overall survivals were analyzed with Kaplan-Meier method and log-rank tests for univariate analysis. RESULTS: Platelet count and PCT were significantly increased, and MPV and P-LCR were significantly reduced in malign and benign gynecological tumor groups compared with the controls (P < .001); PDW had no significant differences. There were no significant differences in PLT, PCT, MPV, P-LCR, and PDW between different tumor locations and pathologic types. The optimal cutoff values of PLT, PCT, MPV and P-LCR were 274, 0.26, 10.08, and 24.8 (AUC: 0.661, 0.643, 0.593, 0.562), and PCT had preferable sensibility and specificity (50.84% and 70.42%) in predicting the presence of gynecological tumors. According to survival analysis, increased PLT (≥274 × 109 /L) and PCT (≥0.26), and induced MPV (<10.08 fL) and P-LCR (<24.8%) were associated with shorter overall survival. CONCLUSIONS: Platelet count, PCT, MPV, and P-LCR can be used as preferable auxiliary parameters for predicting the presence of gynecological tumors. Increased PLT and PCT, or decreased MPV and P-LCR indicated a heavier tumor burden and shorter overall survival.

5.
Stem Cells Dev ; 2020 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-32178579

RESUMO

Photoreceptor transplantation can rescue the retinal function of late-stage rd1 mice. Many studies have used synaptic markers to suggest that there are synaptic connections following transplantation, but how donor and host cell connected remains unknown. Many molecules are needed for triad ribbon synapse formation in wild-type mice. Among them, pikachurin is an important extracellular matrix protein that bridges the pre- and post-synaptic components. To investigate the mechanism of the synaptic connection between donor photoreceptor and host retina, we studied the expression of pikachurin in late-stage rd1 mice before and after transplantation. The results showed that the full-length form of pikachurin could still be detected in the degenerated retina. After photoreceptors were transplanted to the subretinal space of rd1 or wild-type mice, pikachurin was detected in the cytoplasm of most donor photoreceptor cells. Pikachurin puncta may represent the cleaved form of the protein and may indicate synapse generation, but it was barely observed in the donor mass of wild-type mice (3.833.17 puncta per 100 donor cells). In contrast, pikachurin puncta could be found in the graft of the rd1 mouse retina, but the number was low (21.35 9.48 puncta per 100 donor cells). In addition, 54.128.45% of bassoon puncta were paired with pikachurin puncta and 45.56.33% were not, indicating that there were fewer pikachurin puncta than bassoon. These results suggest that pikachurin is involved in only a portion of the synaptic connection between the donor photoreceptor and host retina.

6.
Exp Eye Res ; : 108003, 2020 Mar 14.
Artigo em Inglês | MEDLINE | ID: mdl-32184102

RESUMO

Elevated inflammatory cytokines contribute to the pathogenesis of various retinal diseases such as diabetic retinopathy, retinal vasculitis and retinitis. However, the underlying mechanism of retinal inflammation remains largely unknown. Recent studies demonstrated that acetylcholinesterase (ACHE) is an inflammatory indicator in central neural system. This study was aimed to dissect the role of ACHE in retinal inflammation, and its mechanism of action. Retinal inflammation was induced by intravitreal injection of tumor necrosis factor-α (TNF-α) in heterozygous ACHE knockout mice (ACHE+/-) and wild type mice (ACHE+/+). Donepezil, a well-known ACHE inhibitor, was administrated by daily gavage. Expression of ACHE and intercellular adherent molecule-1 (ICAM-1), infiltration of CD11b+ inflammatory cells, retinal leukostasis and vascular leakage was determined in both ACHE ± and ACHE+/+ mice. ARPE-19 cells, a human retinal pigment epithelial cell line, were cultured for in vitro assay. Knockdown of ACHE was achieved by lipofectamine-mediated siRNA transfection and pharmaceutical suppression of ACHE was manipulated by donepezil. Cellular expression and distribution of ACHE, ICAM-1, and phosphorylation of NF-κB, IκB and IKKα/ß were detected by western-blot analysis or immunocytochemistry. Retinal expression of ACHE was dramatically upregulated, in parallel with increased ICAM-1 expression, enhanced leukostasis and augmented CD11b+ inflammatory cells infiltration as well as vascular hyperpermeability in ACHE+/+ mice injected with TNF-α. However, TNF-α-injected ACHE ± mice showed lower level of ICAM-1, less leukostasis and fewer infiltrated CD11b+ cells. Moreover, TNF-α-induced retinal vascular leakage was significantly reduced in ACHE ± mice. Similarly, TNF-α-induced retinal inflammatory response were also attenuated by donepezil intervention. In addition, TNF-α treatment resulted in significant induction of ACHE, upregulation of ICAM-1 and nuclear translocation of NF-κB in cultured-ARPE-19 cells. Genetic and pharmaceutical suppression of ACHE markedly attenuated TNF-α-induced ICAM-1 expression. Meanwhile, inhibition of ACHE reduced TNF-α-induced phosphorylation of NF-κB, IκB and IKKα/ß in ARPE-19 cells. The present study reveals a pivotal role of ACHE in retinal inflammation. Inhibition of ACHE attenuates retinal inflammation and retinal leakage likely through suppressing NF-κB signaling activation.

7.
Int J Lab Hematol ; 2020 Mar 23.
Artigo em Inglês | MEDLINE | ID: mdl-32202703

RESUMO

INTRODUCTION: Severe aplastic anemia (SAA) is a disease characterized by severe pancytopenia and hematopoietic failure of bone marrow. Natural killer (NK) cells are a class of large granular lymphocytes that perform killing and immunomodulatory functions. Our previous study demonstrated that NK cells played the "protective" role in SAA, which is weakened. However, the mechanism remains unclear. METHODS: Peripheral blood NK cells from SAA patients and normal controls were sorted and total proteins were extracted. Then, mass spectrometry was performed to screen differentially expressed proteins (DEPs). RESULTS: Significant differences in the expression levels of 93 proteins were observed in NK cells of SAA patients compared with normal controls. Among them, 48 were upregulated proteins, including histone H1.2, histone H1.3, heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1), and interferon regulatory factor 1 (IRF-1), and 45 were downregulated proteins, including actin-related complex (ARP2/3), histone H3, histone H4, phosphoglycerate kinase 1 (PGK1), talin-1. Gene Ontology (GO) function indicated that the DEPs most involved were vesicle-mediated transport, innate immune response, and DNA binding. KEGG analysis showed 3 upregulated and 12 downregulated pathways, in which cell endocytosis and FC-γ receptor-mediated phagocytosis were most closely related to NK cell functions. CONCLUSION: Our study is the first analysis of proteomic profile in NK cells in SAA and found many DEPs involving in dysfunction of NK cells, which provides potential targets for deeper research of inadequate immunomodulation.

9.
J Am Chem Soc ; 2020 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-32088950

RESUMO

With the purpose to achieve panchromatic absorption for constructing efficient dye-sensitized solar cells (DSSCs), the cosensitization approach of using two dyes with complementary absorption has been developed with great success. However, this approach usually requires time-consuming optimization of a number of parameters for controlling the ratio and distribution of the two coadsorbed dyes on TiO2 film, which limits the potentials of this strategy. We herein report an alternative approach for developing efficient DSSCs by designing a class of "concerted companion dyes" with two complementary dye components linked covalently. Thus, a newly synthesized organic dye Z2 was linked to a recently reported doubly strapped porphyrin dye XW51 through flexible chains with various lengths to afford XW60-XW63. These dyes exhibit excellent absorption and efficiencies in the range of 8.8%-11.7%. Notably, upon coadsorption with chenodeoxycholic acid, XW61 affords an impressive efficiency of 12.4%, a record for iodine electrolyte-based DSSCs, to the best of our knowledge. In addition, these dyes also exhibit the advantages of easy cell fabrication, simple optimization, as well as excellent photostability.

11.
ACS Nano ; 2020 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-31968166

RESUMO

Two-dimensional (2D) perovskites show great potential for optoelectronic applications due to their bandgap tunability, extremely large excition binding energy, and large crystal anisotropy compared with their three-dimensional counterparts. To fully explore exciton-based applications and improve their performance, it is essential to understand the exciton behavior in 2D perovskites. Here, we investigate exciton anisotropy within the crystallographic plane and cross plane of (C4H9NH3)2PbI4 2D perovskite crystals by polarization-resolved photoluminescence, reflection, and photoconductivity studies. We observe a polarization-dependent emission evolution and an enhanced self-trapped exciton emission with an oblique incident excitation from the cross plane. Furthermore, the anisotropy of excitons in (C4H9NH3)2PbI4 2D perovskite crystals is identified by polarization-resolved photoluminescence and photoconductivity measurement, and a completely opposite polarization-dependent behavior was observed for free excitons and self-trapped excitons. We attribute this different anisotropy to the existence of out-of-plane excitons and different optical selection rule for free excitons and self-trapped excitons. Our findings will shed light on designing and improving the performance of exciton-based optoelectronic devices in 2D perovskites.

12.
Sci Data ; 7(1): 25, 2020 01 21.
Artigo em Inglês | MEDLINE | ID: mdl-31964891

RESUMO

The China Meteorological Forcing Dataset (CMFD) is the first high spatial-temporal resolution gridded near-surface meteorological dataset developed specifically for studies of land surface processes in China. The dataset was made through fusion of remote sensing products, reanalysis datasets and in-situ station data. Its record begins in January 1979 and is ongoing (currently up to December 2018) with a temporal resolution of three hours and a spatial resolution of 0.1°. Seven near-surface meteorological elements are provided in the CMFD, including 2-meter air temperature, surface pressure, and specific humidity, 10-meter wind speed, downward shortwave radiation, downward longwave radiation and precipitation rate. Validations against observations measured at independent stations show that the CMFD is of superior quality than the GLDAS (Global Land Data Assimilation System); this is because a larger number of stations are used to generate the CMFD than are utilised in the GLDAS. Due to its continuous temporal coverage and consistent quality, the CMFD is one of the most widely-used climate datasets for China.

13.
ACS Appl Mater Interfaces ; 12(6): 6955-6965, 2020 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-31977179

RESUMO

The emergence of antibiotic-resistant bacterial strains renders the conventional antibiotic therapy less efficient. The integration of two distinct bactericides into one compact platform provides a promising strategy to realize a combinational antimicrobial therapy. In this work, an efficient chemo-Ag nanohybrid antibacterial platform was facilely developed based on the integration of vancomycin-carrying polydopamine with silver nanoparticles (PDA@Van-Ag). The as-synthesized antibacterial nanohybrid inherited the intrinsic properties of both bactericides to achieve a synergistic antibacterial performance against both Staphylococcus aureus and Escherichia coli strains by attacking bacteria from two distinct fronts. Through this combinational therapy, the efficiency of antibiotic against S. aureus was significantly improved by reducing drug dosage with less opportunity for imparting drug resistance. In addition, this antibacterial nanohybrid, with innate photothermal properties, could achieve auxiliary hyperthermia-assisted bacterial inactivation in the meantime. Furthermore, the outstanding in vivo bacteria-killing activity and wound-healing acceleration were demonstrated in a S. aureus-infected mouse skin defect model. Taken together, this bactericidal nanohybrid could achieve sustained antibiotic release and wipe out bacteria more effectively in a synergistic way, thus reducing the emergence of antibiotic resistance. This work holds great potential to advance the development of novel antibacterial agents and combinational strategies as a promising supplement of antibiotics in the near future.

14.
Biotechnol Bioeng ; 117(2): 342-353, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31654417

RESUMO

The surface display laccase (SDL) biocatalyst, where the enzyme laccase is displayed on the surface of biological cells through synthetic biology, provides a new opportunity to develop sustainable technologies for removal of emerging contaminants from wastewater. This study vigorously characterized biocatalytic properties of the SDL in comparison to free laccase in removing emerging contaminant acetaminophen (APAP), with the aim to understand the effect of surface display on enzyme functionality and identify the strategy to overcome the potential limitation. The SDL could effectively remove APAP. Adding redox mediators substantially improved the removal efficiency. The Michaelis-Menten kinetic analysis showed that the redox mediator 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonate could overcome the limitation of APAP accessing the active site of laccase in the SDL biocatalyst. The APAP removal rate catalyzed by the SDL in real secondary wastewater effluent was higher than that in acetate buffer; comprehensive enzyme kinetic analysis provided clear evidence that there were redox mediating compounds in the wastewater. Analysis of transformation products revealed that surface display did not change laccase functionality in terms of APAP transformation mechanism. In addition, the SDL retained 88% of the initial activity after six repeated APAP biotransformation reactions. Results from this study provide a scientific basis for developing and implementing SDL as an innovative biocatalytic material for contaminant treatment applications.

15.
Plant Cell Environ ; 43(2): 400-419, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31674033

RESUMO

Plants have evolved complex mechanisms that allow them to withstand multiple environmental stresses, including biotic and abiotic stresses. Here, we investigated the interaction between herbivore exposure and salt stress of Ammopiptanthus nanus, a desert shrub. We found that jasmonic acid (JA) was involved in plant responses to both herbivore attack and salt stress, leading to an increased NaCl stress tolerance for herbivore-pretreated plants and increase in K+ /Na+ ratio in roots. Further evidence revealed the mechanism by which herbivore improved plant NaCl tolerance. Herbivore pretreatment reduced K+ efflux and increased Na+ efflux in plants subjected to long-term, short-term, or transient NaCl stress. Moreover, herbivore pretreatment promoted H+ efflux by increasing plasma membrane H+ -adenosine triphosphate (ATP)ase activity. This H+ efflux creates a transmembrane proton motive force that drives the Na+ /H+ antiporter to expel excess Na+ into the external medium. In addition, high cytosolic Ca2+ was observed in the roots of herbivore-treated plants exposed to NaCl, and this effect may be regulated by H+ -ATPase. Taken together, herbivore exposure enhances A. nanus tolerance to salt stress by activating the JA-signalling pathway, increasing plasma membrane H+ -ATPase activity, promoting cytosolic Ca2+ accumulation, and then restricting K+ leakage and reducing Na+ accumulation in the cytosol.

16.
Sci Total Environ ; 708: 134797, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-31784160

RESUMO

The 'Coal Replacing Project' (CRP), replacing coal with cleaner energy like natural gas and electricity, was implemented in North China to curb PM2.5 pollution; therefore, it is important to explore the sources and transformation mechanisms of PM2.5 nitrate under this strategy for examining its effectiveness. In this study, daytime and nighttime PM2.5 samples of one summer (Jul-2016, C1) and two winters (Jan-2017, C2 and Jan-2018, C3, before and during the CRP, respectively) were collected in urban Tianjin. Concentrations of PM2.5 and water-soluble inorganic ions were analyzed, and δ15N and δ18O were used to calculate the contributions of different NOX sources to nitrate based on a Bayesian mixing model. The results showed that the average concentrations of PM2.5 and its dominant inorganic ions (SO42-, NO3-, NH4+) in C3 during the CRP, compared to C2, decreased by 62.13%, 79.69%, 55.14% and 38.84%, respectively, attesting the improvement of air quality during the CRP. According to the correlation between [NO3-/SO42-] and [NH4+/SO42-] as well as δ18O variations, the homogeneous formation pathway might be dominant in C1, while the heterogeneous pathway would be primary in C2 and C3 during the formation of nitrate. Moreover, the heterogeneous pathway contributed more in C3 than in C2. The dominant sources in C1 were biogenic soil emission (37.0% ±â€¯9.9%) and mobile emission (25.7% ±â€¯19.1%), while coal combustion (42.4% ±â€¯13.8% in C2 and 34.9% ±â€¯14.4% in C3) and biomass burning (31.0% ±â€¯21.2% and 34.7% ±â€¯22.7%) were the main sources in C2 and C3. In the winter, the contribution of coal combustion dropped by about 8% during the CRP (C3) in comparison with that in C2, suggesting the implementation of CRP played an important role in reducing NOX from coal combustion.

17.
Org Lett ; 22(2): 433-437, 2020 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-31873035

RESUMO

The first (3 + 2)/(3 + 2) sequential annulation of γ-vinyl allenoates with alkylidenemalononitriles enabled by phosphine catalysis has been reported. A broad range of structurally dense tetra- and penta-substituted bicyclic[3,3,0]octene derivatives, containing a quaternary center and three sequential stereogenic center, were synthesized in good to excellent yields. In this approach, three new C-C bonds are formed in one pot, and εC and αC of γ-vinyl allenoate are two electrophilic centers, whereas its γC exhibits nucleophilic reactivity.

18.
J Am Heart Assoc ; 8(24): e012410, 2019 Dec 17.
Artigo em Inglês | MEDLINE | ID: mdl-31801414

RESUMO

Background Previous studies show that patients with primary aldosteronism are associated with higher risk of congestive heart failure (CHF). However, the effect of target treatment to the incidental CHF has not been elucidated. We aimed to investigate the risk of new-onset CHF in patients with aldosterone-producing adenomas (APAs) and explore the effect of adrenalectomy on new onset of CHF. Methods and Results From 1997 to 2009, 688 APA were identified and matched with essential hypertension controls. The risks of developing incidental CHF (hazard ratio, 0.49; 95% CI, 0.31-0.75; P=0.001) and mortality (hazard ratio, 0.29; 95% CI, 0.20-0.44; P<0.001) were significantly lower in the APA group after targeted treatment. A total of 605 patients with APAs who underwent adrenalectomy lowered the risks of CHF (subdistribution hazard ratio, 0.55; 95% CI, 0.34-0.90; P=0.017) and mortality (adjusted hazard ratio, 0.27; 95% CI, 0.16-0.44; P<0.001) compared with essential hypertension controls. Conclusions In conclusion, for patients with APAs, adrenalectomy can be associated with lower risk of incidental CHF and all-cause mortality in a long-term follow-up.

19.
Cell Signal ; 68: 109493, 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31809872

RESUMO

BACKGROUND: Myelodysplastic syndromes (MDS) represent a family of hematopoietic stem cell disorders characterized by ineffective hematopoiesis. While the functions of many microRNAs have been identified in MDS, microRNA-144 (miR-144) remains poorly understood. Thus, the aim of the present study was to determine the effects of miR-144 on cell proliferation and apoptosis in MDS cells and mechanism thereof. METHODS: MDS-related microarrays were used for screening differentially expressed genes in MDS. The relationship between miR-144 and A-kinase anchoring protein 12 (AKAP12) was determined by a dual luciferase reporter gene assay. Subsequently, gain- and loss-function approaches were used to assess the effects of miR-144 and AKAP12 on cell proliferation, cell cycle and cell apoptosis by MTT assay and flow cytometry. Following the induction of a mouse model with MDS, the tumor tissues were extract for evaluation of apoptosis and the expression of miR-144, AKAP12, and the relevant genes associated with extracellular-regulated protein kinases 1/2 (ERK1/2) signaling pathway and apoptosis. RESULTS: We observed significantly diminished expression of AKAP12 in MDS samples. miR-144 directly bound to AKAP12 3'UTR and reduced its expression in hematopoietic cells. Downregulation of miR-144 or upregulation of AKAP12 was observed to prolong cell cycle, inhibit cell proliferation, and induce apoptosis, accompanied by increased expression of AKAP12, p-ERK1/2, caspase-3, caspase-9, Bax, and p53, as well as decreased expression of Bcl-2. The transplanted tumors in mice with down-regulated miR-144 exhibited a lower mean tumor diameter and weight, and increased apoptosis index and expression of AKAP12 and ERK1/2. CONCLUSION: Taken together, these studies demonstrate the stimulative role of miR-144 in MDS progression by regulating AKAP12-dependent ERK1/2 signaling pathway.

20.
Artigo em Inglês | MEDLINE | ID: mdl-31814556

RESUMO

BACKGROUND: Ginsenoside Rh2 (Rh2) is a major biological component of ginseng that exerts anti-tumor activities in multiply cancers including Non-Small Cell Lung Cancers (NSCLCs). Rh2 also enhance the anti-tumor effects of various chemotherapy drugs including cisplatin at relatively low concentrations. Here, the mechanistic role of Rh2 in chemotherapy-treated NSCLCs will be investigated. METHODS: In this study, FACS, western blot and siRNA addition were used to analyze the role of Rh2 in cisplatin-treated lung adenocarcinoma A549 and H1299 cells. RESULTS: Subsequent observations indicated that Rh2 enhanced cisplatin-induced NSCLCs A549 and H1299 cells apoptosis. Cisplatin-induced productive autophagy was repressed by Rh2 in A549 cells. Rh2 also enhanced cisplatin cytotoxicity by elevating superoxide dismutase activity and repressing cisplatin-induced superoxide generation. Conversely, Rh2 was found to repress cisplatin-induced phosphorylation of epidermal growth factor receptor, phosphoinositide 3-kinase, protein kinase B, and autophagy. Cisplatin-induced Programmed Death-Ligand 1 (PD-L1) expression was repressed by Rh2 via the superoxide. CONCLUSION: These findings suggest that Rh2 enhanced the function of cisplatin by repressing superoxide generation, PD-L1 expression, and autophagy in lung adenocarcinoma cells.

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