Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 388
Filtrar
1.
J Infect ; 2021 May 02.
Artigo em Inglês | MEDLINE | ID: mdl-33951419

RESUMO

OBJECTIVES: Tuberculosis (TB) remains one of the public health problems worldwide. Rapid, sensitive and cost-effective diagnosis of Mycobacterium tuberculosis (M.tb) is critical for TB control. METHODS: We developed a novel M.tb DNA detection platform(nominated as TB-QUICK) which combined loop-mediated isothermal amplification (LAMP) and CRISPR-Cas12b detection. TB-QUICK was performed on pulmonary or plasma samples collected from 138 pulmonary TB (PTB) patients, 21 non-TB patients and 61 close contacts to TB patients. Acid-fast bacillus(AFB) smear, M.tb culture and GeneXpert MTB/RIF (Xpert) assays were routinely conducted in parallel. RESULTS: By targeting M.tb IS6110, TB-QUICK platform could detect as low as 1.3 copy/µL M.tb DNA within 2 hours. In pulmonary TB samples, TB-QUICK exhibited improved overall sensitivity of 86.8% over M.tb culture (66.7%) and Xpert (70.4%), with the specificity of 95.2%.More significantly, TB-QUICK exhibited a superior sensitivity in AFB-negative samples (80.5%) compared to Xpert (57.1%) and M.tb culture (46.2%). In the detection of plasma M.tb DNA by TB-QUICK, 41.2% sensitivity for AFB-positive and 31.7% for AFB-negative patients were achieved. CONCLUSION: In conclusion, TB-QUICK exhibits rapidity and sensitivity for M.tb DNA detection with the superiority in smear-negative paucibacillary TB patients. The clinical application of TB-QUICK in TB diagnosis needs to be further validated in larger cohort.

2.
Turk J Gastroenterol ; 32(2): 209-217, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33960946

RESUMO

BACKGROUND: To investigate the differences between water immersion (WI) and air insufflation (AI) for colonoscopy under various bowel preparation conditions. METHODS: In this study, 526 outpatients were randomly assigned to two groups, namely a WI group (n = 263) and an AI group (n = 263). During the procedure, the quality of bowel preparation, abdominal pain score, cecal intubation rate (CIR), adenoma detection rate (ADR), the intubation times, and other indicators were recorded. After reaching the cecum, each group of patients was subdivided into one of four grades (excellent, good, fair, and poor) according to the quality of bowel preparation. RESULTS: Under various bowel preparation conditions, the pain scores of the AI group were higher than those of the WI group (P < .05), but there was no significant difference between the two groups in CIR (P > .05). For the WI group compared with the AI group, the cecal intubation time (CIT) was prolonged under good bowel preparation (P = .045) and fair bowel preparation (P < .001). No significant differences were observed between the two groups on ADR in all patients (P = .476). CONCLUSION: Compared with AI colonoscopy, WI colonoscopy can decrease colonoscopy-related pain in patients for unsedated colonoscopy under various bowel preparation conditions, but there is no significant difference in CIR. WI colonoscopy requires longer CIT in patients with good and fair bowel preparation conditions. WI colonoscopy does not significantly increase ADR.

3.
Bull Menninger Clin ; : 1-17, 2021 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-33939498

RESUMO

Sleep problems among frontline medical staff during the COVID-19 epidemic require attention. A total of 249 frontline medical staff who were recruited to support Wuhan completed this cross-sectional study. A web-based questionnaire about insomnia, depression, anxiety, and fatigue was used to assess mental health status. The prevalence of sleep disorders among frontline medical staff was 50.6%. More time spent in Wuhan and a history of insomnia, depression, anxiety, and fatigue were associated with a higher risk of insomnia. People who stayed in Wuhan for a long time with a history of insomnia, depression, anxiety, and fatigue symptoms might be at high risk of insomnia.

4.
Environ Pollut ; 285: 117254, 2021 Apr 28.
Artigo em Inglês | MEDLINE | ID: mdl-33957517

RESUMO

Embryonic exposure to environmental chemicals may result in specific chronic diseases in adulthood. Parabens, a type of environmental endocrine disruptors widely used in pharmaceuticals and cosmetics, have been shown to cause a decline in women's reproductive function. However, whether exposure to parabens during pregnancy also negatively affect the ovarian function of the female offspring in adulthood remains unclear. This study aims to investigate the effects of prenatal propylparaben (PrP) exposure on the ovarian function of adult mice aged 46 weeks, which is equivalent to the age of 40 years in women. Pregnant ICR mice were intraperitoneally injected with human-relevant doses of PrP (i.e., 0, 7.5, 90, and 450 mg/kg/day) during the fetal sex determination period-from embryonic day E7.5 to E13.5. Our results revealed that ovarian aging was accelerated in PrP-exposed mice at 46 weeks, with altered regularity of the estrous cycle, decreased serum estrogen (E2) and progesterone (P4) levels, reduced size of the primordial follicle pool, and increased number of atretic follicles. It was found that prenatal exposure to human-relevant doses of PrP exacerbated ovarian oxidative stress, inflammation, and fibrosis, which promoted follicular atresia by activating the mitochondrial apoptosis pathway. To compensate, the depletion of primordial follicles was also accelerated by activating the PI3K/AKT/mTOR signaling pathway in PrP-exposed mice. Moreover, PrP induced hypermethylation of CpG sites in the promoter region of Cyp11a1 (a 17.16-64.28% increase) partly led to the disrupted steroidogenesis, and the altered methylation levels of imprinted genes H19 and Peg3 may also contribute to the phenotypes observed. These remarkable findings highlight the embryonic origin of ovarian aging and suggest that a reduced use of PrP during pregnancy should be advocated.

5.
J Clin Med ; 10(6)2021 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-33808636

RESUMO

One in four American older adults fall every year, resulting in injuries, death, and significant financial burden. Although fall etiology is multifactorial, the medical problems and aging factors that lead to unsteady gait and imbalance represent one of the major fall risks among older adults. A growing number of research studies support the health benefits of regular Tai Chi (TC) practice including improved physical, cognitive, and psychological function. The purpose of this quality improvement project was to assess the feasibility of establishing a 12-week (45 min per session) Tai Chi (TC) program (Sun Style Tai Chi) in a 75 bed assisted living facility as well as to evaluate the potential of the TC program to improve the fear of falling and functional mobility (as proxy for fall risk) and quality of life (QoL). A nurse who was a certified TC instructor taught the program. Twenty-three participants, 96% female and 96% white, mean (SD) age 83 (±7) years, attended one or more TC classes. Class attendance, self-reported questionnaires (e.g., fear of falling, QoL), and objective measure Timed Up and Go (TUG) were used to collect data. Nine participants (39%) completed 9 out of 12 sessions. Eleven participants (48%) completed both pre- and post-intervention measurements and twelve (52%) provided feedback on a post-intervention satisfaction survey. Participants showed 20% improvement in fear of falling (mean relative change) and 21% decrease (mean relative change) in TUG test (p = 0.001) with no clinically important changes in QoL. This quality improvement project suggested that TC is a feasible exercise that might have the potential to reduce risk of falls in older adults, and the program was well accepted with no serious or other adverse events reported. Further research studies are needed to examine the potential effects of TC programs with an appropriately powered RCT and longer intervention period.

6.
Cell Death Dis ; 12(5): 409, 2021 Apr 17.
Artigo em Inglês | MEDLINE | ID: mdl-33866326

RESUMO

The levels of fibroblast growth factor 23 (FGF23) rapidly increases after acute kidney injury (AKI). However, the role of FGF23 in AKI is still unclear. Here, we observe that pretreatment with FGF23 protein into ischemia-reperfusion induced AKI mice ameliorates kidney injury by promoting renal tubular regeneration, proliferation, vascular repair, and attenuating tubular damage. In vitro assays demonstrate that SDF-1 induces upregulation of its receptor CXCR4 in endothelial progenitor cells (EPCs) via a non-canonical NF-κB signaling pathway. FGF23 crosstalks with the SDF-1/CXCR4 signaling and abrogates SDF-1-induced EPC senescence and migration, but not angiogenesis, in a Klotho-independent manner. The downregulated pro-angiogenic IL-6, IL-8, and VEGF-A expressions after SDF-1 infusion are rescued after adding FGF23. Diminished therapeutic ability of SDF-1-treated EPCs is counteracted by FGF23 in a SCID mouse in vivo AKI model. Together, these data highlight a revolutionary and important role that FGF23 plays in the nephroprotection of IR-AKI.

7.
Artigo em Inglês | MEDLINE | ID: mdl-33893914

RESUMO

Financial stability is of great importance especially in the context of achieving sustainable environment. The objective of this study is to fill the research gap in this area by introducing financial stability, international trade, renewable energy, and income as novel determinants of consumption-based carbon emissions. The present study is based on G-7 economies, and the time period is from 1990 to 2018. The present study employed advanced econometric techniques that can deal with problems of slope homogeneity and cross-section dependence. The cointegration analysis results show a stable long-run association between financial stability, renewable energy, international trade, national income, and consumption-based carbon emissions with structural breaks (1994 Italy's fiscal crises, 2001 mild recession, 2008 global financial crises, and 2010 European debt crises). The results show that both in long- and short-run financial stability, exports and renewable energy significantly reduce carbon emissions. In contrast, national income and imports are found to have a significant positive effect on consumption-based carbon emissions. Policymakers in G-7 countries should focus more on financial sector stability and encourage firms to use renewable energy. Any policy that targets financial stability, exports, and renewable energy will significantly reduce carbon emissions. This study is a novel contribution to the area of consumption-based carbon emissions as it incorporates the role of financial stability for G-7 economies.

8.
BMC Gastroenterol ; 21(1): 188, 2021 Apr 23.
Artigo em Inglês | MEDLINE | ID: mdl-33892648

RESUMO

BACKGROUND: Currently, there are no circulating diagnostic biomarkers for gastric neuroendocrine neoplasms (g-NENs). In previous studies, we found that miRNA-202-3p is overexpressed in the tumour tissue of type 1 g-NEN. We speculated that miRNA-202-3p is also likely to be highly expressed in circulating blood. METHODS: A total of 27 patients with type 1 g-NEN and 27 age- and sex-matched control participants were enrolled in this study. The miRNA-202-3p levels in serum obtained from the participants were measured by qRT-PCR. The expression level of miRNA-202-3p in the samples was calculated by comparison with a standard curve. RESULTS: The clinical characteristics of the patients were similar to those of the patient samples in previous reports. Expression of miRNA-202-3p was significantly higher in the patient group (3.84 × 107 copies/nl) than in the control group (0.635 × 107 copies/nl). The area under the ROC curve (AUC) was 0.878 (95% CI: 0.788-0.968), and the optimal cut-off point was approximately 1.12 × 107 copies/nl. The sensitivity and specificity were 88.9% and 77.8%, respectively. CONCLUSION: This study suggests that miRNA-202-3p is potentially useful as a biomarker of type 1 g-NEN; further investigation and verification should be performed in future research.

9.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 29(2): 311-315, 2021 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-33812392

RESUMO

OBJECTIVE: To investigate the clinical features and prognostic factors of acute lymphoblastic leukemia (ALL) children with P2RY8-CRLF2 gene rearrangement. METHODS: A total of 108 children with B-cell ALL (B-ALL) were diagnosed and systematically treated according to Chinese Children's Leukemia Group (CCLG) -ALL 2008 in our hospital from January 2016 to December 2016. The 108 patients were divided into two groups according to the result of mutiplex polymerase chain reaction: group with P2RY8-CRLF2 gene rearrangement and group without P2RY8-CRLF2 gene rearrangement. The ALL children with P2RY8-CRLF2 gene rearrangement were all treated by CCLG-ALL 2008 high-risk group (HR) regimens, and the ALL children in group without P2RY8-CRLF2 gene rearrangement received different intensity chemotherapy according to clinical risk classification. RESULTS: Five (4 male and 1 female) out of 108 patients with B-ALL had P2RY8-CRLF2 gene rearrangement. In the 5 B-ALL patients with P2RY8-CRLF2 gene rearrangement, the median age of the was 4 (2-6) years old and the median WBC count was 26.2 (2.46-525.1)×109/L. These patients presented different immunophenotype, including 3 cases of common B-ALL and 2 cases of pre B-ALL. Four patients carried a normal karyotype and 1 patient carried 46, XY, der (20) [22]/46, XY[2]. For the children with P2RY8-CRLF2 gene rearrangement, 1 patient (20%) could not achieve complete remission (CR), and minimal residual disease (MRD) of 2 patients (40%) was higher than 1% on day 33 of induction chemotherapy; while in group without P2RY8-CRLF2 gene rearrangement, all the patient achieved CR, and MRD in 6 patients (5.8%) was higher than 1% on day 33 of induction chemotherapy. The 3 year event-free survival (EFS) of ALL children in group with P2RY8-CRLF2 gene rearrangement was significantly lower than that in group without P2RY8-CRLF2 gene rearrangement (60.0%±21.9% vs 85.9%±3.9%) (P<0.05). CONCLUSION: The early treatment response and prognosis of ALL children with P2RY8-CRLF2 gene rearrangement are worse, and more effective protocol is needed for this subtype patients.


Assuntos
Leucemia-Linfoma Linfoblástico de Células Precursoras B , Leucemia-Linfoma Linfoblástico de Células Precursoras , Criança , Pré-Escolar , Intervalo Livre de Doença , Feminino , Rearranjo Gênico , Humanos , Masculino , Leucemia-Linfoma Linfoblástico de Células Precursoras B/genética , Leucemia-Linfoma Linfoblástico de Células Precursoras/genética , Prognóstico , Receptores de Citocinas/genética , Receptores Purinérgicos P2Y/genética
10.
Chem Commun (Camb) ; 57(31): 3812-3815, 2021 Apr 21.
Artigo em Inglês | MEDLINE | ID: mdl-33876129

RESUMO

We report the discovery of an acid-assisted de-tert-butylation reaction that can instantly "turn off" the dynamicity of hindered urea bonds (HUBs) and thus broaden their applications. The reaction is demonstrated to be widely applicable to different hindered urea substrates, leading to improved chemical stabilities and mechanical properties of HUB-containing materials.

11.
Zhongguo Zhen Jiu ; 41(4): 391-4, 2021 Apr 12.
Artigo em Chinês | MEDLINE | ID: mdl-33909359

RESUMO

OBJECTIVE: To observe the clinical effect of single acupoint [Yaotu (extra)] electroacupuncture (EA) therapy on lumbar intervertebral disc herniation (LIDH) and its promotion and application in community medical institutions. METHODS: This research adopted a three-level promotion model, and used multi-center collaboration topics as a platform. A total of 240 patients with LIDH were divided into a group A (top three hospital, 80 cases, 3 cases dropped off), a group B (secondary hospital, 80 cases, 8 cases dropped off), and a group C (community health center, 80 cases, 7 cases dropped off). All groups were treated with EA at a single acupoint [Yaotu (extra)] under the guidance of a unified experimental protocol. The EA was given 60 min each time and performed 3 times a week for a total of 2 weeks. The changes of visual analogue scale (VAS) scores of three groups before and after each treatment were compared, and the clinical efficacy was evaluated. RESULTS: Compared with before each treatment, the VAS scores of three groups after each treatment decreased (P<0.05). Except for the second and fifth treatments, the immediate effect in the group A was higher than that in the group B and C (P<0.05). The total effective rate of group A, group B, and group C respectively was 90.9% (70/77), 93.1% (67/72), 86.3% (63/73), and there was no statistically significant difference among the three groups (P>0.05). CONCLUSION: The single acupoint EA therapy has a significant effect in the treatment of LIDH, can quickly relieve the pain symptoms, and has the characteristics of simple operation and easy control, suitable for promotion and application in primary hospitals.


Assuntos
Eletroacupuntura , Degeneração do Disco Intervertebral , Deslocamento do Disco Intervertebral , Disco Intervertebral , Pontos de Acupuntura , Humanos , Deslocamento do Disco Intervertebral/terapia
12.
J Trauma Nurs ; 28(2): 90-99, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33667203

RESUMO

BACKGROUND: Few studies have tracked hand injury patients past the acute care period. Postdischarge tracking of hand injury patients may identify time points most patients require assistance, which can help direct interventions to reduce post-hand injury sequelae. OBJECTIVE: To examine hand injury patients' experiences during early recovery to illness perceptions, disabilities, and quality of life and to identify predictors of quality of life at 1 month and 3 months after hospital discharge. METHODS: This prospective observational study of hand injury patients was performed at a teaching hospital in Taiwan. Data were collected at patient discharge, 1 month, and 3 months after discharge, from January 2017 to October 2018. The variables measured included illness perceptions, disabilities, and quality of life. RESULTS: A total of 117 patients participated in the study. The patients reported more positive illness perceptions at 3 months versus 1 month after discharge. The impact of disabilities on patient work was significantly greater at 1 month than at 3 months after discharge. Quality of life was better at 3 months than at 1 month after discharge. Predictors of quality of life after hospital discharge were demographic variables, clinical variables, and illness perceptions. Patients with lower education reported poorer psychological quality of life after discharge. CONCLUSION: Primary care providers should perform a comprehensive assessment of patients prior to their discharge. Patients should undergo regular follow-ups to reduce comorbidities and improve their outcomes.

14.
Nat Commun ; 12(1): 1572, 2021 03 10.
Artigo em Inglês | MEDLINE | ID: mdl-33692349

RESUMO

Macrocycles are unique molecular structures extensively used in the design of catalysts, therapeutics and supramolecular assemblies. Among all reactions reported to date, systems that can produce macrocycles in high yield under high reaction concentrations are rare. Here we report the use of dynamic hindered urea bond (HUB) for the construction of urea macrocycles with very high efficiency. Mixing of equal molar diisocyanate and hindered diamine leads to formation of macrocycles with discrete structures in nearly quantitative yields under high concentration of reactants. The bulky N-tert-butyl plays key roles to facilitate the formation of macrocycles, providing not only the kinetic control due to the formation of the cyclization-promoting cis C = O/tert-butyl conformation, but also possibly the thermodynamic stabilization of macrocycles with weak association interactions. The bulky N-tert-butyl can be readily removed by acid to eliminate the dynamicity of HUB and stabilize the macrocycle structures.

15.
Pest Manag Sci ; 2021 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-33786962

RESUMO

BACKGROUND: Images and DNA sequences are two important methods for identifying fruit fly species. In addition, the identification of insect species complexes is highly problematic when attempting to utilize automatic identification methods in an actual environment. We integrated the image and DNA sequence identification methods into a single system for the first time and explored an open interactive multi-image comparison function for solving the problem of species complexes. The Automated Fruit Fly Identification System 1.0 (AFIS1.0) was updated to AFIS2.0 by employing different models and developing the system under a novel framework. RESULTS: AFIS2.0 was developed using 83 species belonging to eight genera in the Tephritidae, which includes most pests of this family. The system applies the Mask Region Convolutional Neural Network (Mask R-CNN) and discriminative deep metric learning (AlexNet based) methods for image identification, integrates Blast+ for DNA sequence comparison and specific weighting for the fusion result. At the species level, the best classification success rate for wing images (as the Top 1 species in the species list of outcomes) reached 90%, and the average classification success rate for wing, thorax, and abdomen images (as the Top 5 species in the species list of outcomes) was 94%. CONCLUSION: AFIS2.0 is more accurate and convenient than AFIS1.0 and can be beneficial for users with or without specific expertise regarding Tephritidae. It also provides a more compact and fluent computer system for fruit fly identification, and can be easily applied in practice.

16.
Anal Chim Acta ; 1152: 338282, 2021 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-33648646

RESUMO

Prostate specific antigen (PSA) has become a potential biomarker for detecting prostate cancer (PCa) in the early stage. Herein, we report a target-induced resolution for the detection of PSA sensitively and specifically by amperometric electrochemical measurements. To meet a satisfactory performance, three conformations of pre-design DNA aptamers including two stem-loop structures and a double strand structure have been investigated and compared. All of them are immobilized on gold electrode as capture probes with redox-active molecular. The mechanism of signal transduction depends on molecular recognition events involving aptamer conformational changes, thus influencing the charge transfer. A short, single-stranded DNA (ssDNA) pseudoknot forming two stem-loop structural aptamers with labeled MB at the 3' -terminus was found to posse the highest signal variation than other structure when induced by PSA due to the strong conformational change. With the optimized capture strand, the aptasensor showed the peak current increase of MB by the binding relationship between PSA and the sensor over a wide concentration range of 4 magnitude orders. The proposed aptasensor exhibited a wide detection range from 10 pg/mL to 500 ng/mL with a low detection limit of 1.24 pg/mL (S/N = 3). Moreover, the electrochemical aptasensor demonstrated good reproducibility, sensitivity, selectivity, and reliability for the detection of PSA. We also found the aptasensor had a good response in the human serum samples, making this device easy to operate for the detection of the PSA physiological concentration.

17.
J Hazard Mater ; 414: 125544, 2021 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-33676252

RESUMO

Cefazolin (CFZ) is widely present in the wastewater treatment effluents and in receiving waters and has caused severe impacts to the ecosystem. CFZ degradation by photocatalysis has attracted increasing attention due to its eco-friendly features. Herein, we presented a green synthesis strategy for a highly active BiOBr photocatalyst for CFZ removal with L-cysteine as a directing agent, and the role of thiol in cysteine for facets control and morphology regulation was discussed. We found that the photoactivity of cysteine-induced BiOBr nanosheet was much higher than those prepared by using arginine and glycine as directing agent. Further experiments showed that the cysteine preferentially coordinated Bi3+ with thiol rather than carboxyl. The strong interactions of thiol group with the external surface of the BiOBr crystals stabilize the small crystals that have high surface energy without the cysteine. Such a chemical environment favors forming BiOBr crystalline with small size of high surface area and oriented growth in [110] direction, which facilitates the photogenerated electron-hole separation to achieve significantly promoted photocatalytic activity. Moreover, the cysteine-directed BiOBr nanosheets displayed good photoreactivity to more pollutants (i.e. rhodamine B, cefradine and cefoperazone sodium) and excellent reusability as verified via seven consecutive recycle experiments. The proper photocatalyst dosage (0.4 g/L), relatively acidic water environment (pH 3.4) and high temperature (35 °C) would be beneficial to CFZ photodegrdation by BiOBr. Also, the CFZ photodegradation mechanism and degradation pathway were also proposed by combining radical trapping experiments and electron spin resonance (ESR) test with LC-MS analysis in the photocatalytic process.

18.
Chin Med J (Engl) ; 2021 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-33769968

RESUMO

BACKGROUND: Epigenetics, especially DNA methylation, plays an important role in the pathogenesis of primary Sjogren syndrome (pSS). Our study aimed to reveal the role of DNA methylation in peripheral monocytes of pSS patients. METHODS: A total of 11 pSS patients and five age-matched healthy controls (HCs) were included in this study. Monocytes were isolated from peripheral blood mononuclear cells using magnetic microbeads. DNA methylation profiles were generated using Human Methylation 850K BeadChips. RESULTS: In monocytes from pSS patients, we identified 2819 differentially methylated positions (DMPs), comprising 1977 hypomethylated- and 842 hypermethylated-DMPs, corresponding to 1313 unique genes when compared with HCs. IFI44L, MX1, PAARP9, and IFITM1, which influence the interferon (IFN) signaling pathway, were among the genes hypomethylated in pSS. Functional analysis of genes with a minimum of two DMPs showed involvement in antigen binding, transcriptional regulation, cell adhesion, IFN-γ pathway, type I IFN pathway, antigen presentation, EB virus infection, human T-lymphotropic virus type 1 virus infection, and metabolic disease-related pathways. In addition, patients with higher serum IgG levels exhibited enrichment in Notch signaling and metabolic-related pathways. Upon comparing monocytes with salivary gland epithelial cells, an important overlap was observed in the cell cycle, cell senescence, and interleukin-17 signaling pathways. The differentially methylated genes were more enriched in the ribosome- and AMP-activated protein kinase signaling pathway in anti-Ro/SSA and anti-La/SSB autoantibodies double-positive patients. CONCLUSION: Genome-wide DNA methylation profiling revealed significant differences in DNA methylation in monocytes isolated from patients with pSS.

19.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 29(1): 49-55, 2021 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-33554796

RESUMO

OBJECTIVE: To investigate the clinical effect and safety of Chinese Children's Leukemia Group (CCLG)-ALL 2008 (high risk group) protocol in the treatment with childhood Mixed phenotype acute leukemia (MPAL). METHODS: The clinical data of 15 new diagnosed patients with MPAL treated in our hospital from January 2013 to December 2017 were retrospectively analyzed, and received CCLG-ALL 2008 (high risk group) protocol chemotherapy. RESULTS: One patient gave up treatment after diagnosed, and 14 children with MPAL after induction remission chemotherapy, 3 patients gave up, and 5 patients received consolidation chemotherapy, and 6 patients received allogeneic hematopoietic stem cell transplantation (allo-HSCT). The complete remission (CR) rate was 85.7% at d33 of induction remission chemotherapy. The serious adverse event and treatment-related mortality (TRM) rate was 71.4% and 14.3%, respectively. The recurrence rate was 21.4% and the median time of relapse was 12(9.7-18.4) months. Except for 4 patients who gave up treatment, the 5-year event-free survival (EFS) rate in the other 11 patients was (54.5±15.0)%. The 5 years EFS of 4 patients who received consolidation chemotherapy was significantly lower than the 6 patients who received allo-HSCT after CR (25.0%±21.7% vs 83.3%±15.2%, P=0.033). CONCLUSION: The CCLG-ALL2008 (for high-risk group) protocol in treatment of children with MPAL can get a high CR rate, but also with a high incidence of SAE. The patients received allo-HSCT after CR may have a good prognosis.


Assuntos
Transplante de Células-Tronco Hematopoéticas , Leucemia Mieloide Aguda , Criança , Intervalo Livre de Doença , Humanos , Fenótipo , Prognóstico , Indução de Remissão , Estudos Retrospectivos
20.
Plant Physiol ; 185(4): 1666-1681, 2021 Apr 23.
Artigo em Inglês | MEDLINE | ID: mdl-33569600

RESUMO

Aquaporins such as the plasma membrane intrinsic proteins (PIPs) allow water to move through cell membranes and are vital for stomatal movement in plants. Despite their importance, the dynamic changes in aquaporins during water efflux and influx have not been directly observed in real time in vivo. Here, to determine which factors regulate these changes during the bidirectional translocation of water, we examined aquaporin dynamics during the stomatal immune response to the bacterial flagellin-derived peptide flg22. The Arabidopsis (Arabidopsis thaliana) aquaporin mutant pip2;1 showed defects in the flg22-induced stomatal response. Variable-angle total internal reflection fluorescence microscopy revealed that the movement dynamics and dwell times of AQ6]GFP-AtPIP2;1 in guard cells and subsidiary cells exhibited cell type-specific dependencies on flg22. The cytoskeleton, rather than the cell wall, was the major factor regulating AtPIP2;1 dynamics, although both the cytoskeleton and cell wall might form bounded domains that restrict the diffusion of AtPIP2;1 in guard cells and subsidiary cells. Finally, our analysis revealed the different roles of cortical actin and microtubules in regulating AtPIP2;1 dynamics in guard cells, as well as subsidiary cells, under various conditions. Our observations shed light on the heterogeneous mechanisms that regulate membrane protein dynamics in plants in response to pathogens.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...