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2.
Zhonghua Wai Ke Za Zhi ; 57(12): 902-907, 2019 Dec 01.
Artigo em Chinês | MEDLINE | ID: mdl-31826593

RESUMO

Objectives: To evaluate the efficacy of minimally invasive surgery in patients with late severe tricuspid regurgitation after cardiac surgery, and to evaluate the role of leaflets augmentation technique in tricuspid valvuloplasty. Methods: From January 2015 to June 2019, 85 patients undergoing tricuspid valve repair procedure with minimally invasive approach at Department of Cardiovascular Surgery, Guangdong provincial People's Hospital were enrolled. There were 22 males and 63 females, aging of (53.6±12.4) years (range: 15 to 75 years). The interval between the prior and current operations was (16.0±7.3) years (range: 0.2 to 35.0 years). The diameter of right atrium and right ventricle was (77.3±17.2) mm and (61.0±8.4) mm, respectively. Tricuspid regurgitation was severe or extremely severe, the tricuspid regurgitation area was (19.0±10.3) cm(2). All patients underwent minimally invasive tricuspid valvuloplasty or tricuspid valve replacement on beating-heart with totally endoscopic technique and port-access approach through right chest wall. The operations included tricuspid valve replacement and tricuspid valvuloplasty, the technique of tricuspid valvuloplasty including leaflets augmentation with patch, ring implantation, chordae tendineaes reconstruction, release of papillary muscle, edge to edge method, etc. Postoperative hospitalization days, the time of ICU stay, blood transfusion rate, ventilator time and the results of echocardiography were recorded. Follow-up was completed regularly by WeChat, telephone and outpatient visit. Results: Sixty-five patients underwent tricuspid valve repair, and 20 patients underwent tricuspid valve replacement because of prosthetic failure and plasty failure. Five patients died during hospitalization, with mortality rate 5.9%. One patient was transferred to local hospital for anti-infection treatment, the other 79 patients were discharged from hospital in well condition and followed-up. The postoperative hospitalization time was 7.0 (5.5) days (M(Q(R))) days, the mean ventilator time was 18.0 (16.2) hours, and the mean ICU stay time was 68.0 (75.5) hours. There were 35 patients without blood conduction transfusion, the transfusion rate was only 58.9% (50/85). Four cases of severe, 9 cases of moderate and 67 cases of mild to zero tricuspid regurgitation were examined before being discharged, with tricuspid regurgitation area of (2.8±3.5) cm(2) (range: 0 to 19.1 cm(2)). The follow-up time was 1 to 38 months. Two patients died during follow-up, one patient died from infective endocarditis and mitral perivalvular leakage, the other one died of intractable right heart failure. One patient was implanted with permanent pacemaker due to Ⅲ atrioventricular block. Valvular re-replacement was performed in 2 patients who were re-admitted for the artificial valve infection and mechanical valve obstruction. No re-operation of tricuspid valve. Conclusions: Totally endoscopic minimally invasive technique provided satisfactory surgical outcomes for critically sick patients with severe tricuspid regurgitation following cardiac surgery. The application of leaflets augmentation technique achieved ideal repair effect for previously unrepairable lesions.


Assuntos
Procedimentos Cirúrgicos Cardíacos/efeitos adversos , Insuficiência da Valva Tricúspide/cirurgia , Adolescente , Adulto , Idoso , Procedimentos Cirúrgicos Cardíacos/métodos , Feminino , Implante de Prótese de Valva Cardíaca/efeitos adversos , Implante de Prótese de Valva Cardíaca/métodos , Humanos , Masculino , Pessoa de Meia-Idade , Procedimentos Cirúrgicos Minimamente Invasivos/efeitos adversos , Procedimentos Cirúrgicos Minimamente Invasivos/métodos , Reoperação , Resultado do Tratamento , Valva Tricúspide/cirurgia , Insuficiência da Valva Tricúspide/etiologia , Adulto Jovem
3.
Folia Biol (Praha) ; 65(2): 88-100, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31464184

RESUMO

A single random oligonucleotide 3H primer has been previously applied in random-amplified- polymorphic-DNA (RAPD)-PCR to distinguish stocked bacteria E. coli within a cocktail mixture also containing Enterococcus faecalis, Bifidobacterium longum and Ruminococcus gnavus. In this study, we demonstrate that a 702 base pair (bp) gene fragment can be amplified as a unique pattern by RAPD-PCR using a 3H primer in human faeces containing E. coli. This unique 702 bp amplicon contained a 687 bp gene fragment identified as the C-terminal region of the glutamate-ammonia-ligase adenyltransferase (glnE) gene of E. coli. By high-resolution melt (HRM) analysis, a mean melt-curve temperature of this 702 bp amplicon was determined to be approximately 88.1 ± 0.22 degrees Celsius (°C). A combination of RAPD with HRM in one single reaction based on this amplicon can achieve semi-quantitative detection of up to 102 CFU/ml of E. coli. To increase the signal intensity of HRM, a primer pair capable of screening E. coli directly from fresh human faeces was re-designed from the 687 bp gene segment, giving a mean peak melt-curve temperature at 88.35 ± 0.11 °C. Finally, single-nucleotide polymorphisms of this 687 bp gene segment were analysed for pathogenic E. coli strains, including UMN026, O83:H1, O104:H4, O157:H7 and O169:H41. We conclude that this 687 bp segment of the glnE gene has a high potential for screening of human faecal E. coli, including pathogenic strains, in contaminated food and water.


Assuntos
Primers do DNA/metabolismo , Escherichia coli/enzimologia , Escherichia coli/genética , Genes Bacterianos , Glutamato-Amônia Ligase/química , Glutamato-Amônia Ligase/genética , Técnica de Amplificação ao Acaso de DNA Polimórfico , Sequência de Aminoácidos , Pareamento de Bases/genética , Sequência de Bases , Escherichia coli/isolamento & purificação , Fezes/microbiologia , Glutamato-Amônia Ligase/metabolismo , Humanos , Polimorfismo de Nucleotídeo Único/genética
6.
Zhonghua Bing Li Xue Za Zhi ; 47(7): 531-535, 2018 Jul 08.
Artigo em Chinês | MEDLINE | ID: mdl-29996319

RESUMO

Objective: To investigate the pathologic features of gonadal tissues of disorders of sexual development (DSD) in children. Methods: Fifty-three cases of gonadal developmental disorders were collected from July 2015 to August 2017 at Guangzhou Women and Children's Medical Center. Clinical manifestations, karyotypes, sex hormone levels, ultrasound imaging, histology and immunophenotype of gonadal tissues were analyzed. Results: The age of patients ranged from 7 months to 17 years with an average of (50.7 ± 47.1) months. Social genders of the patients included 32 males and 21 females. Forty-eight patients had abnormal sex hormone levels. Clinical presentations included: toward female genitalia in 25 cases, male genitalia tendency in 17 cases and ambiguous external genitalia in 11 cases. Hypospadias was seen in 31 cases and short stature was seen in 8 cases. Chromosomal karyotyping of peripheral blood revealed 23 cases of sex chromosome disorders, 22 cases of 46 XY disorders, of which 3 cases were 5α-reductase deficiency and 8 cases of 46 XX disorders. Ultrasound examination showed cryptorchidism in 30 cases, including 16 cases of unilateral, 14 cases of bilateral and 1 case presenting a huge pelvic tumor. A total of 97 gonadal tissues from 53 cases of DSD were examined, including 9 cases of unilateral and 44 cases of bilateral gonads. Microscopically, 55 gonads (56.7%) showed dysplastic testes including 17 unilateral and 19 bilateral gonads. Fourteen were streak gonads (14.4%) including 8 unilateral and 3 bilateral gonadal tissues. Nine streak gonad with epithelial cord-like structures (9.3%) were found, of which 5 were unilateral and 2 were bilateral lesions. Seven gonads were ovotestis (7.2%), unilateral in 5 cases (the other side of the gonads of ovary in 4 cases, 1 case of dysplastic testes) and bilateral in 1 case. Seven gonads showed follicular-rich ovarian tissue (7.2%). One case showed bilateral dysplastic testes with gonadoblastoma and ectopic adrenal cortex. One case of streak gonad showed epithelial cord-like structures and undifferentiated glandular tissue embedded in malignant mixed germ cell tumors (mixed gonadoblastoma, dysgerminoma, mature teratoma and yolk sac tumor). One case had testicular microlithiasis. Uterus and fallopian tube structures were found in 11 cases. Immunohistochemical stains were performed in 15 cases. D2-40, PLAP and CKIT were expressed in germ cells and Calretinin, WT1 and inhibin were positive in Setoli cells. SALL4 and OCT3/4 were positive in 3 cases. Inhibin highlighted interstitial Leydig cells in 2 cases. GPC3 was positive in yolk sac tumor component. Conclusions: Gonadal dysgenesis presents a broad spectrum of gonadal phenotypes with variable degrees of differentiation. The development of bilateral gonadal tissues has certain variability. Chromosomal karyotypes have no correlation with gonadal phenotypes. Accurate histopathologic diagnosis of gonadal dysgenesis plays an important role in the treatment and prognosis of the patient.


Assuntos
Transtornos do Desenvolvimento Sexual/patologia , 3-Oxo-5-alfa-Esteroide 4-Desidrogenase/deficiência , Adolescente , Cálculos/patologia , Criança , Pré-Escolar , Transtorno 46,XY do Desenvolvimento Sexual , Tubas Uterinas/patologia , Feminino , Humanos , Hipospadia/patologia , Lactente , Cariotipagem , Masculino , Neoplasias Embrionárias de Células Germinativas , Ovário/anormalidades , Ovário/patologia , Erros Inatos do Metabolismo de Esteroides , Teratoma/patologia , Doenças Testiculares/patologia
7.
Cryo Letters ; 39(1): 7-13, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29734411

RESUMO

  BACKGROUND: Osmotic shock upon the addition and removal of cryoprotectant agent (CPA) is a major source of cell damage during cryopreservation. OBJECTIVE: Microfluidic device offers a new platform for CPA loading and unloading. The micro scale dimension makes possible to perform a detailed analysis and controllable removal of CPA with many advantages. MATERIALS AND METHODS: A microfluidic device was developed for extracting dimethyl sulfoxide (DMSO) from the sample streamline. The device has two parallel channels separated by a polytetrafluoroethylene (PTFE) membrane and serves as the stable environment for CPA removal. A diffusion-based simulation model was used to characterize the CPA extraction. To support the experimental design and device optimization we developed analogous scheme to simulate by COMSOL Multiphysics. RESULTS AND CONCUSION: The device can extract cryoprotectant in a mesoscale volume from cells and simplify the post-thaw sample handling. It has sufficient control on loading/unloading of CPAs by controlling the flow rate of cell stream/wash stream solutions via syringe pumps. Compared to other customary devices, this device is easy to fabricate and assemble, with features of high precision, reusability and low cost.


Assuntos
Custos e Análise de Custo , Criopreservação/instrumentação , Criopreservação/métodos , Crioprotetores/farmacologia , Espaço Extracelular/metabolismo , Dispositivos Lab-On-A-Chip , Microtecnologia/instrumentação , Dimetil Sulfóxido/farmacologia , Espectrofotometria
8.
Artigo em Chinês | MEDLINE | ID: mdl-29081103

RESUMO

Objective: To investigate whether the identification of acrylonitrile, an occupational hazard factor for the industry of injection molding and plastic products, reported in literature is reasonable, and to put forward some recommendations. Methods: Professional articles published from 1990 to 2016 were searched, and an analysis was performed for the data on the determination of acrylonitrile in the industry of injection molding and plastic products from 2003 to 2016 in Longhua Center for Disease Control and Prevention of Shenzhen. Results: According to the literature, the detection rate of acrylonitrile was 10.7%, and the detection results did not exceed the limit. Conclusion: At present, acrylonitrile may not be used as a routine test item for the industry of injection molding and plastic products, in order to save manpower and material resources.


Assuntos
Acrilonitrila/efeitos adversos , Poluentes Ocupacionais do Ar/efeitos adversos , Indústria Química , Exposição Ocupacional/efeitos adversos , Plásticos
10.
Zhonghua Nei Ke Za Zhi ; 56(8): 618-620, 2017 Aug 01.
Artigo em Chinês | MEDLINE | ID: mdl-28789501
11.
Bone Joint Res ; 6(5): 284-295, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-28515058

RESUMO

OBJECTIVES: The lack of effective treatment for cartilage defects has prompted investigations using tissue engineering techniques for their regeneration and repair. The success of tissue-engineered repair of cartilage may depend on the rapid and efficient adhesion of transplanted cells to a scaffold. Our aim in this study was to repair full-thickness defects in articular cartilage in the weight-bearing area of a porcine model, and to investigate whether the CD44 monoclonal antibody biotin-avidin (CBA) binding technique could provide satisfactory tissue-engineered cartilage. METHODS: Cartilage defects were created in the load-bearing region of the lateral femoral condyle of mini-type pigs. The defects were repaired with traditional tissue-engineered cartilage, tissue-engineered cartilage constructed with the biotin-avidin (BA) technique, tissue-engineered cartilage constructed with the CBA technique and with autologous cartilage. The biomechanical properties, Western blot assay, histological findings and immunohistochemical staining were explored. RESULTS: The CBA group showed similar results to the autologous group in biomechanical properties, Moran's criteria, histological tests and Wakitani histological scoring. CONCLUSIONS: These results suggest that tissue-engineered cartilage constructed using the CBA technique could be used effectively to repair cartilage defects in the weight-bearing area of joints.Cite this article: H. Lin, J. Zhou, L. Cao, H. R. Wang, J. Dong, Z. R. Chen. Tissue-engineered cartilage constructed by a biotin-conjugated anti-CD44 avidin binding technique for the repairing of cartilage defects in the weight-bearing area of knee joints in pigs. Bone Joint Res 2017;6:-295. DOI: 10.1302/2046-3758.65.BJR-2016-0277.

12.
Andrology ; 5(4): 798-806, 2017 07.
Artigo em Inglês | MEDLINE | ID: mdl-28544569

RESUMO

The pathophysiology of erectile dysfunction post radical prostatectomy is not clearly clarified, and the low efficacy of traditional PDE5i treatment remains a major complaint in contemporary practice. This study aimed to demonstrate phenotypic modulation in bilateral cavernous nerve injury (BCNI) rats within 7 days, and subsequently validate gene therapy with Myocardin (Mycod) by maintaining a contractile phenotype in corpus cavernosum smooth muscle cells. Initially, 36 male rats were randomly divided into BCNI and negative control (NC) groups for histological and phenotypic molecular measurements at 3, 5, and 7 days. Afterwards, an additional 30 rats received a single intra-cavernous injection of 50 µL PBS, Ad-Myocd (1 × 1011  pfu/ml) or Ad-vector for 10 animals each, namely the NC+PBS, BCNI+Ad-Myocd, and BCNI+Ad-vector groups. Finally, the validity and mechanism of Myocd transfection was explored at 21 days in vivo and 48 h in vitro. Western blotting showed canonical declines in Myocd, α-SMA, and Calponin expression, as well as elevated Osteopontin (OPN) expression, before corporeal morphological and SM-to-collagen ratio changes at day 5 after injury. Overexpression of Myocd maintained the contractile phenotype of corpus cavernosum smooth muscle cells, ameliorated bilateral cavernous nerve injury rat erectile dysfunction, as well as promoted cell contractility and suppressed proliferative capacity. Simultaneously, confocal imaging revealed up-regulation and co-localization of serum response factor in gene-transferred cells. In conclusion, our study is the first to investigate corpus cavernosum smooth muscle cells phenotypes in the early stages of cavernous injury model rats, and Myocd reversed phenotypic modulation by activating serum response factor. The experimental results demonstrated the validity of gene therapy for erectile dysfunction.


Assuntos
Disfunção Erétil/terapia , Terapia Genética/métodos , Contração Muscular , Músculo Liso/inervação , Miócitos de Músculo Liso/metabolismo , Compressão Nervosa , Proteínas Nucleares/biossíntese , Ereção Peniana , Pênis/inervação , Transativadores/biossíntese , Actinas/metabolismo , Animais , Proteínas de Ligação ao Cálcio/metabolismo , Células Cultivadas , Modelos Animais de Doenças , Disfunção Erétil/genética , Disfunção Erétil/metabolismo , Disfunção Erétil/fisiopatologia , Masculino , Proteínas dos Microfilamentos/metabolismo , Músculo Liso/metabolismo , Proteínas Nucleares/genética , Osteopontina/metabolismo , Pênis/metabolismo , Fenótipo , Ratos Sprague-Dawley , Fatores de Tempo , Transativadores/genética
13.
Zhonghua Bing Li Xue Za Zhi ; 45(4): 252-7, 2016 Apr 08.
Artigo em Chinês | MEDLINE | ID: mdl-27033389

RESUMO

OBJECTIVE: To study the clinicopathologic features of pediatric vascular anomalies and application of ISSVA classification. METHODS: The clinical features, histopathologic findings and immunohistochemical results were analyzed in 117 cases of pediatric vascular anomalies encountered during the period from May 2014 to May 2015. RESULTS: A total of 117 cases of vascular anomalies were studied. The age of patients ranged from 18 hours after birth to 11 years (mean age =34 months and median age =27 months). There were 73 male patients and 44 female patients, with the male-to-female ratio being 1.7∶1.0. Congenital skin lesions were found in 37 cases (31.6%). The common sites of involvement included head and neck region (46 cases, 39.3%), trunk (28 cases, 23.9%), extremities (14 cases, 12.0%) and internal viscera (31 cases, 26.5%). According to the new ISSVA classification, there were 74 cases of vascular malformations and 43 cases of vascular neoplasms (ratio=1.7∶1.0). The commonest vascular tumor encountered was infantile hemangioma (21 cases, 48.8%), including 17 cases in proliferative phase and 4 cases in involutive phase. Thirteen cases (23.3%) of congenital hemangioma were found, with 8 cases of rapidly involuting congenital hemangioma and 5 cases of non-involutive congenital hemangioma. Three of the congenital hemangioma occurred in liver. There were 5 cases (11.6%) of pyogenic granuloma, 3 cases (7.0%) of tufted angioma and 1 case (2.3%) of Kaposiform hemangioendothelioma. Amongst the 74 cases of vascular malformations encountered, lymphatic malformation was found in 47 cases (63.5%), venous malformation in 15 cases (20.2%), lymphatic-venous malformation in 11 cases (14.9%) and arteriovenous malformation in 1 case (1.4%). All cases of vascular anomalies were all positive for CD31 on immunostaining. Glut1 and CD15 were positive both in proliferative and involutive phases of the 21 cases of infantile hemangioma, while other vascular tumors and vascular malformations were negative. Forty-seven cases of lymphatic malformation and 11 cases of lymphatic-venous malformation showed D2-40 expression. Focal positivity for D2-40 was demonstrated in 3 cases of tufted angioma and 1 case of Kaposiform hemangioendothelioma. CONCLUSIONS: Vascular anomalies affecting infants and children include tumors and malformations. Accurate histopathologic diagnosis and ISSVA classification of the various types of vascular anomalies play an important role in clinical management.


Assuntos
Malformações Vasculares/patologia , Neoplasias Vasculares/patologia , Malformações Arteriovenosas/patologia , Criança , Pré-Escolar , Feminino , Transportador de Glucose Tipo 1 , Hemangioendotelioma/patologia , Hemangioma/patologia , Hemangioma Capilar/patologia , Humanos , Lactente , Recém-Nascido , Síndrome de Kasabach-Merritt/patologia , Masculino , Sarcoma de Kaposi/patologia , Neoplasias Cutâneas/patologia
14.
Zhonghua Xin Xue Guan Bing Za Zhi ; 44(3): 220-5, 2016 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-26988676

RESUMO

OBJECTIVE: To observe the clinical characteristics and outcomes of patients with acute aortic dissection (AAD) and explore the impact of hypertension. METHODS: The present study enrolled 1 087 consecutive patients with AAD who were confirmed by computed tomographic scanning in Fuwai Hospital from January 2008 to December 2010. The major endpoints were in-hospital death and long-term mortality during follow up. RESULTS: A total of 595 (54.7%) patients were Stanford type A and 492 (45.3%) patients were Stanford type B. The median length of follow-up was 24.2 months (interquartile range 10.9, 40.8 months). The prevalence of hypertension was 67.4%(733 cases), and was significantly higher in type B patients than in type A patients (71.3%(351/492) vs. 64.2%(382/595), P=0.01). Regardless of Stanford classification, patients complicating with hypertension were older, had higher comorbidities (coronary heart diseases or diabetes), and less likely to receive surgical treatment compared with those without hypertension (all P<0.05). In Stanford type A AAD group, patients with hypertension had higher levels of admission blood pressure, serum creatinine and inflammatory markers (including WBC count, D-dimer and CRP) than those without hypertension (all P<0.05). In-hospital death (9.9% (38/382)vs. 5.6%(12/213), P=0.07) and long-term mortality (9.0% (31/344) vs. 8.9% (18/201), P=0.98) were similar in hypertensive and normotensive AAD type A patients. In type B AAD group, the in-hospital death rate was significantly higher in patients with hypertension than those without hypertension (5.4%(19/351) vs. 0.7%(1/141), P=0.02), while the long-term mortality was similar (6.9%(23/332) vs. 7.9%(11/140), P=0.71) between patients with and without hypertension. Multiple logistic regression analysis showed that hypertension did not predict the increased risk of in-hospital death of type A or type B AAD patients. The main protective factor of in-hospital mortality was operation in patients with type A AAD. The independent predictors of in-hospital death were age and surgical treatment in patients with type B AAD. CONCLUSIONS: Hypertension is a common co-morbidity in patients with AAD. AAD patients with hypertension are usually elder, have higher comorbidities of cardiovascular diseases, and less likely to receive surgical treatment compared with those without hypertension, but hypertension is not associated with increased risk of in-hospital and long-term mortality in both AAD type A and type B patients.


Assuntos
Aneurisma Dissecante , Aneurisma Aórtico , Doença Aguda , Aneurisma da Aorta Torácica , Pressão Sanguínea , Produtos de Degradação da Fibrina e do Fibrinogênio , Mortalidade Hospitalar , Humanos , Hipertensão
15.
Genet Mol Res ; 14(3): 10705-16, 2015 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-26400300

RESUMO

Many studies exist concerning the use of stem cells as delivery vehicles in gene therapy, expressing genes such as vascular endothelial growth factor 165 and hepatocyte growth factor. However, few reports regarding adipose tissue-derived stem cells (ADSCs) and the heme oxygenase 1 (HO-1) gene have been published. Therefore, we established a lentiviral vector encoding HO-1 and used this to infect ADSCs with the aim of producing therapeutic seed cells. In this study, ADSCs were isolated from mouse adipose tissue (AT), cultured, and identified according to the expression of antigens on their cell surface and their capacity for multilineage differentiation. A lentiviral vector encoding HO-1 was constructed, ADSCs were infected with this, and HO-1 protein expression was examined by western blotting. Our results show that ADSCs can be isolated from mouse AT, while DNA sequencing demonstrated that HO-1 was successfully transferred to the vector fused with GFP. Following 293T cell transfection, lentivirus titers were approximately 3 x 10(8) TU/mL. Fluorescence microscopy confirmed the expression of the HO-1 construct in lentivirus-infected ADSCs and the overexpression of HO-1 protein in these cells was verified by western blot. The production of ADSCs overexpressing HO-1 described in this study may aid in the development of a novel method for the treatment of asthma.


Assuntos
Adipócitos/metabolismo , Tecido Adiposo/metabolismo , Vetores Genéticos/química , Heme Oxigenase-1/genética , Lentivirus/genética , Proteínas de Membrana/genética , Células-Tronco/metabolismo , Adipócitos/citologia , Tecido Adiposo/citologia , Animais , Diferenciação Celular , Expressão Gênica , Genes Reporter , Vetores Genéticos/metabolismo , Proteínas de Fluorescência Verde/genética , Proteínas de Fluorescência Verde/metabolismo , Células HEK293 , Heme Oxigenase-1/metabolismo , Humanos , Proteínas de Membrana/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Cultura Primária de Células , Análise de Sequência de DNA , Células-Tronco/citologia , Transfecção
16.
Hippokratia ; 17(1): 20-6, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-23935339

RESUMO

BACKGROUND: Mycoplasma Pneumoniae (M. pneumoniae) is a common cause of respiratory tract infections (RTIs), especially in children. Combined diagnostic techniques have provided more reliable information about the epidemiology of infections by this pathogen. The relationship between M. pneumoniae RTIs and climatic conditions is not well documented in the literature. AIMS: To study the epidemiology of M. pneumoniae infections in hospitalized children with RTIs and its association with meteorological factors. METHODS: Samples were obtained from children with RTIs and tested for M. pneumoniae by PCR and ELISA. Meanwhile, meteorological factors were recorded. RESULTS: M. pneumoniae was identified in 11.02% of the 8,157 specimens. There were significant differences among the annual distribution of infections (χ(2) =130.13, P<0.0001) and among different seasons (χ(2) =93.59, P<0.0001). Of the total number of patients with M. pneumoniae infections, 14.5% were infected with more than one pathogen. M. pneumoniae infection strongly correlated with mean temperature. Children with a single M. pneumoniae infection had significantly higher neutrophil percentages and CRP levels than children with co-infections. CONCLUSIONS: M. pneumoniae is one of the most commonly held pathogens, according to the 5-year surveillance. M. pneumoniae infection has its own epidemic season, especially in the summer. Mean temperature is the main meteorological factor affecting the epidemiology of M. pneumoniae infections.

17.
Burns ; 24(5): 455-9, 1998 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-9725687

RESUMO

We investigated nitric oxide and cyclic GMP production in myocardium early after burn injury in rats. Nitric oxide synthase activity was measured in cytosol from the left ventricular wall of burned rats. Cytosol from control group animals was shown to contain mainly Ca2+-dependent nitric oxide synthase (cNOS) with a small amount of Ca2+-independent nitric oxide synthase (iNOS). Following burn injury, there was a marked increase in iNOS activity with a peak at 8 h post-burn, however, myocardial cNOS activity was found to decline. Parallel to iNOS induction there was a significant increase in myocardial nitric oxide and cyclic GMP production. All these changes were alleviated by treatment of the rats with dexamethasone. Since increases in cyclic GMP levels in the heart were associated with reduced myocardial contractility, it is possible that enhanced production of nitric oxide by a Ca2+-independent NO synthase accounts, at least in part, for the depression of myocardial contractility seen in burn animals and patients.


Assuntos
Queimaduras/metabolismo , Miocárdio/metabolismo , Óxido Nítrico Sintase/metabolismo , Óxido Nítrico/biossíntese , Animais , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/uso terapêutico , Queimaduras/tratamento farmacológico , GMP Cíclico/biossíntese , GMP Cíclico/metabolismo , Dexametasona/farmacologia , Dexametasona/uso terapêutico , Modelos Animais de Doenças , Masculino , Óxido Nítrico Sintase/efeitos dos fármacos , Técnicas de Cultura de Órgãos , Ratos , Ratos Wistar , Valores de Referência , Espectrofotometria
18.
Microsurgery ; 16(8): 542-6, 1995.
Artigo em Inglês | MEDLINE | ID: mdl-8538431

RESUMO

The effects of topical GM1 ganglioside on crushed rat sciatic nerve regeneration were studied in this presentation. Thirty-four rats, with 68 bilateral sciatic nerves, were divided into seven groups: one group of four rats for measurement of normal motor nerve conduction velocity (MNCV), three as controls, and another three in the GM1-treated groups. Sciatic nerves were exposed and crushed at a site 6 mm distal to the sciatic notch by the standard technique. In this manner, 3 mm wide crush injuries were created. Then 2.1 microliters of normal saline was injected into the crush site in the control groups and an equal volume of GM1 solution (containing 10 micrograms GM1) was injected into the GM1-treated groups. Electrophysiological, histological, and morphometric evaluations were carried out at 12, 28, and 56 days. A significantly higher muscle action potential (MAP) rate was found in the GM1-treated group (70%) vs. the controls (none) at 12 days (P < 0.005), and increased MNCV was found in the GM1-treated groups at both 28 and 56 days, especially at 56 days, when it was 39.59 +/- 9.23 m/sec vs. 31.42 +/- 4.07 m/sec in controls (P < 0.05). Morphometrically, there were more regenerated myelinated fibers (RMFs) at 12 days, and larger diameter of RMFs were observed at 12, 28, and 56 days in the GM1-treated groups.


Assuntos
Gangliosídeo G(M1)/farmacologia , Regeneração Nervosa/efeitos dos fármacos , Nervo Isquiático/fisiologia , Potenciais de Ação , Administração Tópica , Animais , Gangliosídeo G(M1)/administração & dosagem , Masculino , Músculo Esquelético/inervação , Músculo Esquelético/fisiologia , Compressão Nervosa , Condução Nervosa , Ratos , Ratos Wistar , Nervo Isquiático/lesões , Cloreto de Sódio/administração & dosagem , Cloreto de Sódio/farmacologia , Fatores de Tempo
19.
Burns ; 20(4): 311-5, 1994 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-7945819

RESUMO

The inhibitory sites of component D1, isolated by extracting burn eschar with ethyl acetate, on rat liver mitochondrial respiratory chain was investigated. By using changes of the redox state of NADPH in various respiratory states, the effect of D1 on electron flow through the phosphorylation site 1 was studied. It was found that D1 did not interfere with electron transport through site 1, nor did it inhibit the activity of NADH:duroquinone reductase. The functional state of site 2 was tested directly by using duroquinol as an artificial electron donor and cytochrome c as electron acceptor. D1 inhibited the activity of this enzyme in a dose-dependent manner. The redox state of the electron carrier cytochrome b was not influenced by D1. However, a transient process of oxidation of cytochrome c1 was demonstrated, suggesting a brief inhibitory process in the reducing side of it. Two inhibitory sites in site 2 were found by studying the TMPD and DCIP bypasses. One was at the reducing side of cytochrome c1, and the other was at the reducing side of cytochrome b. Further studies are needed to find out the exact position and nature of these inhibitory sites.


Assuntos
Queimaduras/metabolismo , Mitocôndrias Hepáticas/metabolismo , Extratos de Tecidos/farmacologia , Animais , Benzoquinonas/metabolismo , Citocromos/metabolismo , Transporte de Elétrons , NADH Desidrogenase/metabolismo , NADP/metabolismo , Oxirredução , Consumo de Oxigênio/efeitos dos fármacos , Fosforilação , Quinona Redutases/metabolismo , Ratos , Ratos Wistar
20.
Zhongguo Zhong Xi Yi Jie He Za Zhi ; 14(8): 474-7, 1994 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-7841755

RESUMO

This paper studied the effect of Salvia miltiorrhiza (SM) on plasma lipid peroxidation (LPO), RBC superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activity in patients with chronic cor pulmonale. 30 patients were randomly divided into two treatment groups (routine and SM). The four parameters have been measured in 22 healthy subjects as control and patients of two treatment groups. The results showed: (1) Before treatment both treatment groups had significantly higher LPO and SOD and markedly lower GSH-Px and CAT level than that of healthy control (P < 0.001) respectively. (2) After treatment in routine group the four parameters have normalized, P < 0.05. (3) Routine treatment was compared with SM treatment, except SOD, the other three parameters were significantly different between two treatment groups (P < 0.001). The increased levels of GSH-Px and CAT and the decreased LPO were significantly greater in SM group than routine treatment (P < 0.001). The above-mentioned results indicated patients with cor pulmonale in acute exacerbation their lipid peroxide reaction was enhanced, the antioxidant enzymes lost their balance. SM could attenuate markedly lipid peroxide reaction, adjust the imbalance of the three antioxidant enzymes, enhance body's defence capability against damage of active oxygen free radical induced lipid peroxidation.


Assuntos
Catalase/sangue , Medicamentos de Ervas Chinesas/uso terapêutico , Glutationa Peroxidase/sangue , Peróxidos Lipídicos/sangue , Doença Cardiopulmonar/tratamento farmacológico , Adulto , Feminino , Humanos , Masculino , Extratos Vegetais , Doença Cardiopulmonar/sangue , Salvia miltiorrhiza
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