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1.
Opt Express ; 29(3): 3011-3025, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-33770909

RESUMO

We theoretically investigate one-dimensional localized gap modes in a coherent atomic gas where an optical lattice is formed by a pair of counterpropagating far-detuned Stark laser fields. The atomic ensembles under study emerge as Λ-type three-level configuration accompanying the effect of electromagnetically induced transparency (EIT). Based on Maxwell-Bloch equations and the multiple scales method, we derive a nonlinear equation governing the spatial-temporal evolution of the probe-field envelope. We then uncover the formation and properties of optical localized gap modes of two kinds, such as the fundamental gap solitons and dipole gap modes. Furthermore, we confirm the (in)stability regions of both localized gap modes in the respective band-gap spectrum with systematic numerical simulations relying on linear-stability analysis and direct perturbed propagation. The predicted results may enrich the nonlinear horizon to the realm of coherent atomic gases and open up a new door for optical communication and information processing.

2.
Spectrochim Acta A Mol Biomol Spectrosc ; 252: 119526, 2021 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-33582438

RESUMO

In this work, a dual-functional Cu2+-based ensemble (2S·Cu2+) was well designed and characterized. Then, the successional and discriminating sensing for CN- over other competitive species (H2PO4- and biothiols) was achieved based on the disaggregation of 2S·Cu2+ ensemble and the deprotonation of imidazole NH of regenerated sensor S in aqueous solution, respectively. The visual sensing mechanism could be clearly demonstrated by 1H NMR, HRMS and energy changes between the HOMO-LUMO band gaps. Furthermore, the reversibility and reusability of S and 2S·Cu2+ upon alternating addition of CN-/H+ and CN-/Cu2+ were studied. Interestingly, the sequential sensing for biothiols (cysteine, glutathione and homocysteine) and CN- was also realized through spectroscopic methodology and test paper strips. This work may provide a feasible strategy to discriminate CN- over H2PO4- and biothiols with high selectivity and sensitivity through Cu2+-based ensembles.

3.
Biophys J ; 120(5): 752-753, 2021 Mar 02.
Artigo em Inglês | MEDLINE | ID: mdl-33571433
4.
Artigo em Inglês | MEDLINE | ID: mdl-33388997

RESUMO

Previously we reported that administration of IgG could inhibit tumor progression in mouse models. At the same time, we also found that some IgGs have glycosylation modifications on their Fab fragments, which may have different biological functions than non-glycosylated IgG. In this study, we employed mouse tumor models to explore the roles of two different forms of IgG, i.e. Fab-glycosylated and Fab-non-glycosylated IgG, in tumor progression. The two types of IgGs were separated with ConA absorption which could react with glycan on the Fab arm but could not access glycan on the Fc fragment. In addition, we performed cytokine array, ELISA, western blotting, immunocytochemistry and other techniques to investigate the possible mechanisms of the actions of Fab-glycosylated IgG in the models. We found that Fab-glycosylated IgG, unlike Fab-non-glycosylated IgG, did not inhibit tumor growth and metastasis in the model. On the contrary, Fab-glycosylated IgG may bind to antigen-bound IgG molecules and macrophages through the glycosidic chain on the Fab fragment to affect antigen-antibody binding and macrophage polarization, which are likely to help tumor cells to evade the immune surveillance. A new mechanism of immune evasion with Fab-glycosylated IgG playing a significant role was proposed.

5.
Aging (Albany NY) ; 122020 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-33288738

RESUMO

Eph receptors constitute the largest family of RTKs, and their associations with antitumor immunity and immunotherapy are largely unknown. By integrating genomic, transcriptomic and clinical data from cohorts in public databases, we identified EPHA5 as the most common mutated gene of Eph receptors in lung adenocarcinoma (LUAD). Moreover, compared with EPHA5 wild-type (WT) patients, EPHA5-mutant (Mut) patients exhibited significantly enhanced infiltration of CD8+ T cells and M1 macrophages, reduced recruitment of immunosuppressive regulatory T cells (Tregs) into the tumor site, as well as the increased level of chemokine, interferon-gamma, inhibitory immune checkpoint signatures, tumor mutation burden (TMB) and tumor neoantigen burden (TNB). Additionally, EPHA5 mutation cooccurred with homologous recombination (HR) or mismatch repair (MMR) gene mutations. These data were validated in the LUAD cell line H1299 and a Chinese LUAD cohort. Most importantly, clinical analysis of a Memorial Sloan Kettering Cancer Center (MSKCC) immunotherapy cohort indicated that LUAD patients with EPHA5 mutations who were treated with immunotherapy had markedly prolonged survival times. Our results revealed the correlation of EPHA5 mutations with tumor immune microenvironment and predictive factors for immunotherapy, implying the potential of EPHA5 mutations as a prognostic marker for the prognosis of LUAD patients to immune checkpoint blockade therapy.

6.
Protein Expr Purif ; 179: 105788, 2020 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-33221504

RESUMO

MEF2D-fusions have recently been identified as one of the major oncogenic drivers in precursor B-cell acute lymphoblastic leukemia (B-ALL). More importantly, they are often associated with patients with poor prognosis in B-ALL. To have a better understanding of the pathogenic mechanism underpinning MEF2D-fusions-driven leukemogenesis, it's essential to uncover the related structure information. In this study, we expressed and purified the MEF2D N-terminal DNA binding domain. The recombinant protein was engineered by cloning the encoding gene into the expression vector pET-32 m. A series of chromatographic steps involving affinity, ion-exchange and gel-filtration chromatography were used to achieve a final purity of >95%. For the crystallization of the MEF2D-DNA complex, a double-stranded DNA encoding 5'-AACTATTTATAAGA-3' and 5'-TTCTTATAAATAGT-3' was used (Wu et al., 2010) [1]. The MEF2D-DNA crystal with the size of about 20 µm × 20 µm × 20 µm was obtained at a final concentration of 12 mg/ml at the reservoir condition containing 30% PEG1500. The X-ray examination showed that the MEF2D-DNA crystal diffracted to 4.5 Å resolution, and belonged to space group P1, with unit-cell parameters of a = 77.2 Å, b = 77.2 Å, c = 231.4 Å.

7.
J Environ Sci (China) ; 98: 1-13, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33097139

RESUMO

Rainfall samples were collected from three observation sites in Guilin from 2013 to 2017, and the chemical composition characteristics of precipitation and the contribution made by different ion sources were analyzed when atmospheric pollutants levels were reduced. The results showed that acid gas emissions and atmospheric pollutant concentrations continued to decline during the study period. However, the change in the volume-weighted mean pH at the three sites suggested that acid rain pollution was not alleviated and began to deteriorate after 2015. The continuing downward trend for alkaline neutralizing ions (Ca2+, NH4+) in precipitation indicated that the reduction in alkaline neutralizing substances in the atmosphere was an important factor that led to the deterioration in acid rain across Guilin. The principal component analysis and spearman correlation analysis indicated five sources of ions in precipitation. Quantitative assessment of these five sources indicated that fossil fuel combustion contributed the most ions concentration in precipitation at the three sites, followed by agriculture, terrestrial (crustal) sources, marine sources, and biomass burning. Long-distance airflow might affect the acidity, the electrical conductivity (EC), and ion concentrations in precipitation across Guilin. The airflow trajectory from the west and southeast directions corresponded to higher acidity and ion concentrations. According to the current air pollution control strategy planned by Guilin, reducing atmospheric coarse particles and NH3 at the same time may potentially lead to further deteriorations in acid rain contents. Therefore, Guilin needs to develop more reasonable pollution prevention measures that synergistically control atmospheric pollutants and acid rain pollution.


Assuntos
Chuva Ácida , Poluentes Atmosféricos , Poluentes Ambientais , Poluentes Atmosféricos/análise , Atmosfera/análise , Monitoramento Ambiental
8.
Opt Lett ; 45(19): 5616-5619, 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-33001962

RESUMO

We report on an experimental investigation of the five vibrational Raman lines at 358 nm, 388 nm, 391 nm, 428 nm, and 471 nm of N2+ resonantly driven by the self-seeding ionic lasers generated by a polarization-modulated (PM) or alternatively a linearly polarized (LP) femtosecond laser. It was found that the spectral intensities of several Raman lines can be dramatically enhanced by exploiting the PM laser pulses in comparison to the LP laser pulses. The evaluated Raman conversion efficiency reaches ∼10-2 for some lasing lines at suitable pressures. Moreover, the role of interplay between the seed amplification and the resonant vibrational Raman scattering processes in inducing the gain of N2+ lasing is characterized for the first time. The developed vibrational Raman spectroscopy with intense ultrafast lasers provides an additional approach to interrogate the products in a femtosecond filament, and it therefore can be a powerful tool for identifying chemical species at remote distances in the atmosphere.

9.
Ann Transl Med ; 8(16): 998, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32953798

RESUMO

Background: Esophageal squamous cell carcinoma (ESCC) is one of the most lethal cancer due to insufficient actionable molecules. Radiotherapy (RT) plays a vital role in the treatment of ESCC, while radioresistance is a significant challenge to RT and results in locoregional and distant failure. Methods: Radioresistance is a complex involving confounding factors, and its genetic mechanism is challenging to study. Postoperative recurrence after RT is more likely to be due to genetic causes than recurrence in unoperated patients. Therefore, two independent cohorts of ESCC patients who had received postoperative radiotherapy (PORT) and had opposite prognoses were set up, and whole-exome sequencing (WES) technology was applied. We compared the differences in the mutant spectra between the two groups. Results: The mutation rate was slightly higher in the relapsed group than in the stable group [average mutation rate, 1.15 vs. 0.73 mutations per megabyte (Mb)], while the mutation types and proportions in the two groups were not significantly different. In particular, three mutated genes (TTN, MUC19, and NPIPA5) and two copy number alterations (CNAs) (1q amplification and 14q deletion) were identified to be associated with poor RT prognosis, while MUC4 was a favorable factor. Conclusions: These radioresistance biomarkers may supply insight into predicting the radioresponse. Further, these findings offer the first data on the mutational landscape of ESCC radioresistance.

10.
Neoplasma ; 2020 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-32940041

RESUMO

Most lung cancer deaths are caused by a distant disseminated disease rather than primary tumors. Understanding the biology behind distant metastasis (DM) is crucial for the effective prediction and reduction of recurrence rates. Genome-wide analysis of the tumor provides a new way to explore the pathogenesis and molecular diagnosis of metastasis in lung adenocarcinoma. In our study, a total of 215 eligible lung adenocarcinoma patients were enrolled. The DNA was extracted from Formalin-fixed paraffin-embedded (FFPE) samples from the primary tumors of these patients. Comprehensive molecular profiling was performed using a panel covering the exome of lung cancer-associated driver genes based on targeted next-generation sequencing. Tumor gene alterations were analyzed to investigate the differences in molecular features between lung adenocarcinomas with or without DM. Patients with DM of lung adenocarcinoma had significantly more variations in overall copy number (defined as Copy Number Alteration (CNA) load and Copy Number Instability (CNI) score). Interestingly, the study of the relationship between copy number variation and other molecular features verified that the degree of copy number variation has a positive correlation with mutations of DNA damage repair pathway (DDR). Thus, the additional analysis further revealed that metastatic patients accumulated more mutations in the DDR pathway, suggesting that impaired function of the DDR pathway and copy number variations play important roles in the invasion process of cancer cells. A comprehensive genetic analysis of lung adenocarcinoma revealed significant genomic changes between DM and non-DM patients. This finding may shed new light on the elucidation of lung cancer invasion mechanisms, and provide potential predictors for metastatic lung cancer.

11.
J Hazard Mater ; 399: 123088, 2020 11 15.
Artigo em Inglês | MEDLINE | ID: mdl-32937718

RESUMO

An effective strategy for enhancement of catalytic activity and stability of immobilized laccase via metal affinity adsorption on Fe3O4@C-Cu2+ nanoparticles was developed, which involved the fabrication of hydroxyl and carboxyl functionalized Fe3O4@C nanoparticles via a simple hydrothermal process and the subsequent chelation with Cu2+ for the immobilization of laccase under a mild condition. Our results revealed that the Fe3O4@C-Cu2+ nanoparticles possess a high loading amount of bovine serum albumin (BSA, 436 mg/g support) and laccase activity recovery of 82.3 % after immobilization. Laccase activity assays indicated that thermal and pH stabilities, and resistances to organic solvents and metal ions of the immobilized laccase were relatively higher than those of the free enzyme. The immobilized laccase maintained more than 61 % of its original activity after 10 consecutive reuses. Most importantly, the immobilized laccase possessed excellent degradation of diverse synthetic dyes. The degradation rates of malachite green (MG), brilliant green (BG), crystal violet (CV), azophloxine, Procion red MX-5B, and reactive blue 19 (RB19) was approximately 99, 93, 79, 88, 75 and 81 (%) in the first cycle. Even after 10 consecutive reuses, the removal efficiencies of the six dyes were found to be 94, 80, 71, 78, 60, and 65 (%), respectively.

12.
Mycopathologia ; 2020 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-32865705

RESUMO

Deficiency of caspase recruitment domain-containing protein 9 (CARD9) is an autosomal recessive primary immunodeficiency disorder, which typically predisposes immunocompetent individuals to single fungal infections and multiple fungal infections are very rare. We study an otherwise healthy 48-year-old man, who had been admitted to our hospital diagnosed with deep dermatophytosis caused by Trichophyton rubrum for three times at 29, 33 and 48 years old, respectively. At the age of 39 years, he suffered from cutaneous mucormycosis due to Mucor irregularis. Moreover, he had a long history of superficial fungal diseases and occasional oral candidiasis. Whole-exome sequencing revealed two compound heterozygous splicing variants in CARD9 gene, c. 184 + 5 G > T and c. 951G > A, confirmed by Sanger sequencing. Patients with recurrent fungal infections especially invasive fungal infections in the absence of known immunodeficiencies should be tested for CARD9 mutations.

13.
Ann Transl Med ; 8(14): 884, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32793728

RESUMO

Background: Ranking fourth in the world in tumor incidence and second in cancer-related death worldwide, gastric cancer (GC) is one of the major malignant tumors, and has a very complicated pathogenesis. In the present study, we aimed to identify new biomarkers to predict the survival rate of GC patients. Methods: The differentially expressed genes (DEGs) between GC tissues and normal stomach tissues were obtained by using GEO2R, and overlapped DEGs were acquired with Venn diagrams. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were conducted with R software. Then, the protein-protein interaction (PPI) of these DEGs was visualized by Cytoscape. Gene Expression Profiling Interactive Analysis (GEPIA) was used to verify the expression differences of hub genes in gastric adenocarcinoma tissues and normal tissues. Overall survival (OS) of hub genes was calculated by Kaplan-Meier plotter. Results: There were a total of 128 consistently expressed genes in the two datasets: 85 upregulated genes were enriched in extra-cellular matrix (ECM)-receptor interaction, protein digestion and absorption, focal adhesion, gastric acid secretion, mineral absorption, systemic lupus erythematosus, amoebiasis, and PI3K-Akt signaling pathway, and 43 downregulated genes were enriched in palate development, blood coagulation, positive regulation of transcription from RNA polymerase II promoter, axonogenesis, receptor internalization, negative regulation of transcription from RNA polymerase II promoter, and in no significant signaling pathways. From the PPI network analyzed by Molecular Complex Detection (MCODE) plug-in, all 27 upregulated genes were selected. Furthermore, to analyze the OS among these genes, Kaplan-Meier analysis was conducted, and 25 genes were associated with remarkably worse survival. For validation in GEPIA, 11 of 25 genes were discovered to be highly expressed in GC tissues compared to normal OS tissues. Furthermore, in the re-analysis of the Database for Annotation, Visualization and Integrated Discovery (DAVID), three genes [G2/miotic-specific cyclin B1 (CCNB1), polo-like kinases 1 (PLK1), and pituitary tumor-transforming gene-1 (PTTG1)] were markedly enriched in the cell cycle pathway, particulary the G1-G1/S phase. Conclusions: Three remarkably upregulated DEGs with poor prognosis in GC were identified and may serve as new prognostic biomarkers and targets in GC therapy.

14.
Injury ; 51(11): 2617-2621, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32807393

RESUMO

PURPOSE: The purpose of this study was to evaluate the correlation of the bone mineral density (BMD) of the hip and lumbar spine with the distal radius cortical thickness (DRCT) measured on anteroposterior radiographs and establish a method for predicting osteoporosis. METHODS: We assessed 147 patients aged ≥50 years with distal radius fractures who underwent wrist radiographs and dual-energy X-ray absorptiometry. The DRCT was measured and calculated at two levels of the distal radius of the injured wrist on the radiographs. RESULTS: The intra-rater and inter-rater reliability of measures was excellent (intraclass correlation coefficient >0.85). In the Pearson correlation and simple linear regression analyses, the DRCT was positively correlated with hip BMD (r = 0.393, P < 0.01) and lumbar spine BMD (r = 0.529, P < 0.01). Each 1-mm increase in DRCT was associated with a 0.051-g/cm2 increase in hip BMD (R2 = 0.154, P < 0.01) and a 0.080-g/cm2 increase in lumbar spine BMD (R2 = 0.280, P < 0.01). A DRCT of 5.1 mm was selected as the cutoff point for predicting osteoporosis, with the highest Youden index of 0.560, 83.3% sensitivity, 72.7% specificity, and a 66.7% negative predictive value. CONCLUSION: Cortical thickness measurements obtained from anteroposterior wrist radiographs were positively correlated with hip and lumbar spine BMD measurements. This technique is suggested as a rapid, inexpensive, and sensitive method for predicting osteoporosis. LEVEL OF EVIDENCE: Diagnostic II.

15.
Opt Express ; 28(15): 22829-22843, 2020 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-32752537

RESUMO

We investigate the formation of rotational coherence of N2+ resonantly interacting with an intense femtosecond laser field by numerical simulations based on a strong-field ionization-coupling model described with the density matrix formalism. The created N2+ system is unique in many aspects: the variable total population within the pump duration due to the intensity-dependent ionization injection, the readily accessible resonance owing to the effect of Stark shift, and the involvement of a few dozen of quantum states. By regarding the N2+ system as an open and non-stationary Λ-type cascaded multi-level system, we quantitatively studied the dependence of rotational coherence in different electronic-vibrational states of N2+ on the alignment angle θ and the pumping intensity. Our simulation results indicate that the quantum coherence between the neighbouring rotational states of J, J+2 in the vibrational state ν=0, 1 of the ground state of N2+ can be changed from a negative to a positive. The significant contribution of rotational coherence to inducing an extra gain or absorption of N2+ air lasing is further verified by solving the Maxwell's propagating equation. The finding provides crucial clues on how to manipulate N2+ lasing by controlling the rotational coherence and paves the way to studying strong-field quantum optics effects such as lasing without inversion and electromagnetically induced transparency in molecular ionic systems.

16.
J Cell Biol ; 219(9)2020 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-32770195

RESUMO

Clathrin-mediated endocytosis occurs via the assembly of clathrin-coated pits (CCPs) that invaginate and pinch off to form clathrin-coated vesicles (CCVs). It is well known that adaptor protein 2 (AP2) complexes trigger clathrin assembly on the plasma membrane, and biochemical and structural studies have revealed the nature of these interactions. Numerous endocytic accessory proteins collaborate with clathrin and AP2 to drive CCV formation. However, many questions remain as to the molecular events involved in CCP initiation, stabilization, and curvature generation. Indeed, a plethora of recent evidence derived from cell perturbation, correlative light and EM tomography, live-cell imaging, modeling, and high-resolution structural analyses has revealed more complexity and promiscuity in the protein interactions driving CCP maturation than anticipated. After briefly reviewing the evidence supporting prevailing models, we integrate these new lines of evidence to develop a more dynamic and flexible model for how redundant, dynamic, and competing protein interactions can drive endocytic CCV formation and suggest new approaches to test emerging models.

17.
Cancer Cell Int ; 20: 388, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32831648

RESUMO

Background: Esophageal squamous cell carcinoma (ESCC) is one of the most frequent malignant tumors originated from digestive system around the world and the treatment was limited by the unclear mechanism. DNA polymerase epsilon 2, accessory subunit (POLE2) is involved in DNA replication, repair, and cell cycle control, whose association with ESCC is still not clear. Methods: In this study, the expression level of POLE2 in ESCC tissues was detected by IHC. The POLE2 knockdown cell line was constructed, identified by qPCR and western blot and used for detecting cellular functions and constructing xenotransplantation mice model. MTT Assay, colony formation assay, flow cytometry, wound-healing assay and Transwell assay were used to detected cell proliferation, apoptosis and migration. Results: We firstly identified that the expression of POLE2 was overexpressed in ESCC. Moreover, the high expression of POLE2 can predict the tumor deterioration and poor prognosis of ESCC patients. Additionally, downregulation of POLE2 was involved in ESCC progression by promoting proliferation, migration, and inhibiting apoptosis in vitro. In vivo studies proved that POLE2 was positively correlated with ESCC tumor formation, which was consistent with the results in vitro. We also illuminated that POLE2 knockdown upregulated pro-apoptotic proteins (Bax, Caspase3, CD40L, FasL, IGFBP-5 and P21) and downregulated anti-apoptotic proteins (CLAP-2, IGF-I and sTNF-R2). In addition, POLE2 was involved in ESCC via targeting PI3K/Akt, Cyclin D1 signaling pathway. Conclusions: Therefore, POLE2 was proved to be involved in the development of ESCC, which may be a potential therapeutic target and bring new breakthroughs in the treatment of ESCC.

18.
Traffic ; 21(9): 603-616, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32657003

RESUMO

Clathrin mediated endocytosis (CME) has been extensively studied in living cells by quantitative total internal reflection fluorescence microscopy (TIRFM). Fluorescent protein fusions to subunits of the major coat proteins, clathrin light chains or the heterotetrameric adaptor protein (AP2) complexes, have been used as fiduciary markers of clathrin coated pits (CCPs). However, the functionality of these fusion proteins has not been rigorously compared. Here, we generated stable cells lines overexpressing mRuby-CLCa and/or µ2-eGFP, σ2-eGFP, two markers currently in use, or a novel marker generated by inserting eGFP into the unstructured hinge region of the α subunit (α-eGFP). Using biochemical and TIRFM-based assays, we compared the functionality of the AP2 markers. All of the eGFP-tagged subunits were efficiently incorporated into AP2 and displayed greater accuracy in image-based CCP analyses than mRuby-CLCa. However, overexpression of either µ2-eGFP or σ2-eGFP impaired transferrin receptor uptake. In addition, µ2-eGFP reduced the rates of CCP initiation and σ2-eGFP perturbed AP2 incorporation into CCPs and CCP maturation. In contrast, CME and CCP dynamics were unperturbed in cells overexpressing α-eGFP. Moreover, α-eGFP was a more sensitive and accurate marker of CCP dynamics than mRuby-CLCa. Thus, our work establishes α-eGFP as a robust, fully functional marker for CME.

19.
J Cell Biol ; 219(9)2020 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-32520988

RESUMO

Clathrin-mediated endocytosis (CME) occurs via the formation of clathrin-coated vesicles from clathrin-coated pits (CCPs). Clathrin is recruited to CCPs through interactions between the AP2 complex and its N-terminal domain, which in turn recruits endocytic accessory proteins. Inhibitors of CME that interfere with clathrin function have been described, but their specificity and mechanisms of action are unclear. Here we show that overexpression of the N-terminal domain with (TDD) or without (TD) the distal leg inhibits CME and CCP dynamics by perturbing clathrin interactions with AP2 and SNX9. TDD overexpression does not affect clathrin-independent endocytosis or, surprisingly, AP1-dependent lysosomal trafficking from the Golgi. We designed small membrane-permeant peptides that encode key functional residues within the four known binding sites on the TD. One peptide, Wbox2, encoding residues along the W-box motif binding surface, binds to SNX9 and AP2 and potently and acutely inhibits CME.

20.
Vasc Med ; 25(5): 436-442, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32558619

RESUMO

This study aimed to investigate the expression and diagnostic value of miR-106b-5p in asymptomatic carotid artery stenosis (CAS) patients, and further explore its predictive value for the occurrence of cerebral ischemic events (CIE). A total of 58 asymptomatic CAS cases and 61 healthy controls were recruited. Quantitative RT-PCR was applied for the measurement of the miR-106b-5p level. The receiver operating characteristic (ROC) curve was plotted to assess the diagnostic value of miR-106b-5p for CAS. Kaplan-Meier methods and Cox regression analysis were performed to assess the predictive value of miR-106b-5p for the occurrence of CIE. In patients with asymptomatic CAS, miR-106b-5p was highly expressed. The miR-106b-5p level showed a significant association with dyslipidemia, hypertension, and the degree of carotid stenosis. miR-106b-5p had a relative accuracy in differentiating patients with asymptomatic CAS from healthy individuals, with a sensitivity of 89.7% and specificity of 83.6% at the cutoff value of 0.198. Patients with high miR-106b-5p expression experienced more CIE. miR-106b-5p was highly expressed in patients with asymptomatic CAS. Our present results provide evidence for miR-106b-5p as a promising biomarker for CAS diagnosis, and for predicting the risk of future CIE in patients with asymptomatic CAS.

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