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1.
Front Endocrinol (Lausanne) ; 14: 1133566, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36950698

RESUMO

Embryo selection in in vitro fertilization-embryo transfer (IVF-ET) mostly relies on morphological assessment using a conventional microscope or the time-lapse monitoring system, which is not comprehensive. Inappropriate levels of reactive oxygen species (ROS) in the fertilization medium may cause damage to gametes, eventually leading to adverse IVF outcomes. The present study aimed to identify the optimal oxidation-reduction level in the fertilization medium for IVF outcomes by measuring the static oxidation-reduction potential (sORP) using a highly accurate and sensitive MiOXSYS system. A total of 136 patients undergoing IVF following brief incubation were divided equally into 4 groups in this prospective cohort study. The sORP value in the fertilization medium was detected using the MiOXSYS system, and its relationship with IVF outcomes was analyzed. The primary outcome was pregnancy outcomes, including live birth rate (LBR), clinical pregnancy rate (CPR), biochemical pregnancy rate (BPR), and implantation rate (IR). The secondary outcome was embryo quality, including fertilization rate (FR), cleavage rate (CR), available embryo rate (AER), and good-quality embryo rate (GQER). Group II (sORP: 228.7-235.3 mV) showed a higher LBR, CPR, BPR, and IR compared with Group III (sORP: 235.4-242.7 mV), presented as follows: LBR (32.0% for Group II vs 3.6% for Group III, P = 0.033), CPR (32.0% for Group II vs 3.6% for Group III, P = 0.033), BPR (36.0% for Group II vs 3.6% for Group III, P = 0.019), and IR (31.3% for Group II vs 2.7% for Group III, P = 0.003). The FR in Groups I and II had lower significant differences compared with that in Groups III and IV (71.7% and 70.3% for Groups I and II vs 83.5% and 80.4% for Groups III and IV, P = 0.000). The GQER in Group I to Group IV was 32.7%, 37.4%, 26.5%, and 33.3%, respectively (P = 0.056). This study indicated that the sORP value in the fertilization medium might be a potential indicator of embryo quality and pregnancy outcome.


Assuntos
Fertilização In Vitro , Complicações na Gravidez , Gravidez , Feminino , Humanos , Espécies Reativas de Oxigênio , Estudos Prospectivos , Transferência Embrionária , Fertilização
2.
Eur J Med Res ; 28(1): 28, 2023 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-36642707

RESUMO

BACKGROUND: Progestins can suppress endogenous luteinising hormone (LH) secretion from the pituitary gland and have shown similar efficacy in terms of collecting competent oocytes and embryos; however, some inconsistencies have been proposed regarding the quality of embryos collected with the use of progestins. This study aimed to evaluate euploidy rates and pregnancy outcomes in preimplantation genetic testing for aneuploidy (PGT-A) cycles using the progestin-primed ovarian stimulation (PPOS) protocol versus the gonadotropin-releasing hormone (GnRH) agonist/antagonist protocol. METHODS: This retrospective cohort study included 608 PGT-A cycles: 146 women in the PPOS group, 160 women in the GnRH agonist group, and 302 women in the GnRH antagonist group. This study was performed at the in vitro fertilisation (IVF) centre of Shanghai First Maternity and Infant Hospital between January 2019 and December 2021. Additionally, 267 corresponding first frozen embryo transfer (FET) cycles were analysed to assess pregnancy outcomes. RESULTS: The euploid blastocyst rate per injected metaphase II(MII) oocytes (14.60% vs. 14.09% vs. 13.94%) was comparable among the three groups (p > 0.05). No significant differences were observed among the three groups regarding pregnancy outcomes, including biochemical pregnancy, clinical pregnancy, ongoing pregnancy, implantation, miscarriage, ectopic pregnancy, and live birth rates per transfer in the first FET cycles (p > 0.05). CONCLUSIONS: The PPOS protocol had no negative effect on euploid blastocyst formation, and the pregnancy outcomes in FET cycles using the PPOS protocol were similar to those of the GnRH agonist and antagonist protocols. Trial registration This trial was retrospectively registered.


Assuntos
Aneuploidia , Testes Genéticos , Progestinas , Feminino , Humanos , Gravidez , China , Hormônio Liberador de Gonadotropina/agonistas , Hormônio Liberador de Gonadotropina/antagonistas & inibidores , Estudos Retrospectivos , Esteroides , Resultado da Gravidez , Técnicas de Reprodução Assistida
3.
J Gastrointest Oncol ; 13(5): 2660-2666, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-36388683

RESUMO

Background: Bevacizumab combined with fluorouracil is the currently recommended maintenance treatment for metastatic colorectal cancer, but the use of bevacizumab needs to be carried out in hospitals, which invisibly increases the risk of patients' exposure to coronavirus disease 2019 (COVID-19) during the COVID-19 epidemic. Therefore, except of the advantage of convenience, all oral drugs as the maintenance treatment can reduce hospitalization and potential exposure risk during the COVID-19 epidemic, which is worth further exploration. Case Description: First case was a 49-year-old male with stage IV colon adenocarcinoma and abnormal liver function who was given bevacizumab with FOLFOXIRI (8-cycles), following which his liver function recovered. Oxaliplatin was stopped upon thrombocytopenia development. The patient was finally maintained on oral fruquintinib and capecitabine therapy since November 2020, and has been progression-free for >15 months. Grade 2 leukopenia, neutropenia, and thrombocytopenia; grade 1 terminal nerve injury; and grade 1 hand and foot numbness were observed. The second case was a 48-year-old male with advanced colon cancer who underwent laparoscopic sigmoidectomy. Post-surgery, the patient was commenced on fluorouracil and leucovorin (1-cycle), followed by conversion therapy with cetuximab and chemotherapy (6-cycles). The patient underwent left hemi-hepatectomy, partial hepatectomy of the right lobe, and intraoperative radiofrequency ablation, following which he continued to receive cetuximab and chemotherapy. The patient was maintained on oral fruquintinib and capecitabine since December, 2020 and has been progression-free for >14 months. Grade1 myelosuppression, leukopenia, and neutropenia, grade 2 thrombocytopenia were observed. Conclusions: This case report based on preliminary evidence advocates oral fruquintinib-capecitabine maintenance treatment as an alternative to bevacizumab-capecitabine standard therapy for CRC patients, especially in the era of COVID-19 epidemic. This scheme can reduce hospitalization and potential COVID-19 contact, and is more convenient than intravenous administration. Which should be further explored in future studies.

4.
Front Endocrinol (Lausanne) ; 13: 928024, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35937844

RESUMO

Adenomyosis is a common benign uterine lesion that is associated with female infertility, reduced clinical pregnancy rate and high miscarriage risk. While it has been known that the impaired endometrial receptivity is implicated in infertility in patients with adenomyosis, the underlying mechanism remains unclear. In the present study, we showed that intracellular protein level of IL-33 was downregulated in the endometrium of patients with adenomyosis, and IL-33 expression status was shown to be positively correlated with that of HOXA10, an endometrial receptivity marker. The subsequent analysis indicated IL-33 overexpression led to the increase of HOXA10 expression and enhancement of embryo implantation in vitro, which was accompanied with induction of STAT3 phosphorylation. Meanwhile, cryptotanshinone, a potent STAT3 inhibitor, was found to significantly suppress the increase of HOXA10 expression and embryo implantation caused by IL-33 overexpression in vitro, revealing the critical role of STAT3 activity. Consistently, the positive relationship between IL33 and HOXA10 expression in the endometrium was verified in the analysis of adenomyosis mouse model.


Assuntos
Adenomiose , Infertilidade Feminina , Interleucina-33/genética , Adenomiose/complicações , Animais , Implantação do Embrião , Endométrio , Feminino , Humanos , Infertilidade Feminina/etiologia , Infertilidade Feminina/metabolismo , Interleucina-33/metabolismo , Camundongos , Gravidez
5.
Clin. transl. oncol. (Print) ; 24(8): 1631–1642, agosto 2022.
Artigo em Inglês | IBECS | ID: ibc-206250

RESUMO

PurposeTo explore the regulatory effect of let-7a-5p/TGFBR1/Smad3 on the proliferation activity of cervical cancer cells.MethodsThe difference in let-7a-5p expression between normal people and patients with cervical cancer was detected by miREIA assay. The differences of let-7a-5p expression between cervical cancer cell line C33a and adjacent normal epithelial cell line HUCEC were determined by qRT-PCR.ResultsmiREIA result showed that let-7a-5p concentrations were 178.5 ± 24.3 μg/L in healthy individuals and 106.1 ± 14.8 μg/L in cervical cancer patients (P = 0.0002). qRT-PCR showed that let-7a-5p in cervical cancer tissue (0.57 ± 0.03) was lower than that in adjacent normal tissue (0.84 ± 0.04, P = 0.0107). Compared with normal cervical epithelial cells (HUCEC), the expression of let-7a-5p was lower in cervical cancer cells (C33a, Hela, P = 0.0001). The results of CCK-8 and EDU detection showed that activation of let-7a-5p inhibited the proliferation of C33a (P = 0.00130, P << 0.0001) and Hela (P = 0.00254, P = 0.0066) cells. According to the analysis using Starbase V2.0 online database, let-7a-5p could target TGFβR1 in cervical cancer cell lines, and the let-7a-5p mimic reduces the mRNA expression level of TGFβR1 in cervical cancer cell C33a (P = 0.0067). Western blot results showed that TGFBR1 expression significantly decreased in cervical cancer cells after let-7a-5p mimic treatment (P = 0.0048) and significantly increased after let-7a-5p mimic inhibitor treatment (P = 0.0003).Conclusionslet-7a-5p represents the independent novel anti-oncogenes in cervical cancer, which can regulate TGF-β1/TGFBR1/pSmad3 cell pathway and interfere with the proliferation of cervical cancer cells. Therefore, let-7a-5p can serve as a novel potential therapeutic target for the treatment of cervical cancer. (AU)


Assuntos
Humanos , Linhagem Celular Tumoral , Proliferação de Células , MicroRNAs/genética , RNA Mensageiro , Proteína Smad3/genética , Neoplasias do Colo do Útero/genética
7.
Arch Gynecol Obstet ; 306(5): 1607-1615, 2022 11.
Artigo em Inglês | MEDLINE | ID: mdl-35904610

RESUMO

AIMS: The various diagnostic criteria for polycystic ovary syndrome (PCOS) raised problem for PCOS research worldwide. PCOS has been demonstrated to be significantly associated with immune response. We aimed to identify several immune-related biomarkers and construct a nomogram model for diagnosis in PCOS. METHODS: The mRNA expression data were downloaded from Gene Expression Omnibus (GEO) database. Significant immune-related genes were identified to be the biomarkers for the diagnosis of PCOS using random forest model (RF), support vector machine model (SVM) and generalized linear model (GLM). The key biomarkers were selected from the optimal model and were utilized to construct a diagnostic nomogram. Receiver operating characteristic (ROC) curves was used to evaluate diagnostic ability of nomogram. Moreover, the relative proportion of 22 immune cell types was calculated by CIBERSORT algorithm. RESULTS: Four immune-related biomarkers (cAMP, S100A9, TLR8 and IL6R) were demonstrated to be highly expressed in PCOS. The nomogram constructed on the ground of the four key biomarkers showed perfect performance in diagnosis of PCOS, whose AUC were greater than 0.7. Higher infiltrating abundance of neutrophils, resting NK cells and activated dendritic cells were observed in PCOS and were tightly associated with the four key biomarkers. CONCLUSIONS: This study identified several immune-related diagnostic biomarkers for PCOS patients. The diagnostic nomogram constructed based the biomarkers provide a theory foundation for clinical application. Multiple immune cells were associated with the expression of these four biomarkers and might played a vital role in the procession of PCOS.


Assuntos
Síndrome do Ovário Policístico , Biomarcadores , Feminino , Humanos , Síndrome do Ovário Policístico/diagnóstico , Síndrome do Ovário Policístico/genética , RNA Mensageiro , Curva ROC , Receptor 8 Toll-Like
8.
Environ Sci Pollut Res Int ; 29(60): 91255-91267, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-35882734

RESUMO

Cadmium (Cd) and fluorine (F) often coexist in environment and are toxic to organisms; however, their combined effects on plants are still not well documented. In this study, the co-effects of Cd and F on germination, biomass, photosynthesis, and nutrients uptake of lettuce were carried out in hydroponic culture. The results showed that the seed germination and seedling biomass decreased with an increase in Cd and F supplementation. The root morphology verified these effects as excess combined Cd and F diminished the root tips and surface area of lettuce, while single Cd and F inhibited the growth by decreasing root length and average diameter, respectively. These effects were also consistence with a reduction in photosynthesis which was mainly regulated by reducing the quantum yield of PS II, electron transport activity, stomatal conductance, intercellular CO2 concentration, and transpiration rate in response to the pollutants. Moreover, when lettuce exposed to Cd and F stress, the accumulation of several essential elements in shoot decreased. In a sum, the synergistic negative effects of Cd and F on the seed germination and seedling growth of lettuce were observed, and these might be owed to nutrient absorption and translocation in the plant. These findings aid in understanding the harmful effects and specific mechanisms of action of Cd and F on plants.

10.
Artigo em Inglês | MEDLINE | ID: mdl-35679110

RESUMO

Two aerobic and obligately acidophilic bacteria, designated 4G-K13T and 4Y35T, were isolated from the forest soil sampled at Dinghushan Biosphere Reserve, Guangdong Province, PR China. These two strains were Gram-stain-negative, non-motile and short rods that multiplied by binary division. Strains 4G-K13T and 4Y35T had the highest 16S rRNA gene sequence similarity of 97.0 and 97.2 % to Silvibacterium bohemicum DSM 103733T and Acidisarcina polymorpha SBC82T, respectively. Phylogenetic trees based on the 16S rRNA gene and whole genome sequences showed consistently that these two strains formed a major clade with members of the genera Acidipila, Acidisarcina, Silvibacterium and Acidobacterium in the family Acidobacteriaceae, but each occupied an unique position. In both the UBCG and the PhyloPhlAn phylogenomic trees, strains 4G-K13T and 4Y35T congruently formed a highly supported subclade with Acidobacterium capsulatum DSM 11244T and Acidobacterium ailaaui DSM 27394T, respectively. The major fatty acids (>5 %) of strain 4G-K13T were iso-C15 : 0, iso-C17 : 0, summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and summed feature 9 (iso-C17 : 1 ω9c and/or C16 : 0 10-methyl), while that of strain 4Y35T were C16 : 0, C18 : 1 ω9c, iso-C15 : 0, summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and summed feature 9 (iso-C17 : 1 ω9c and/or C16 : 0 10-methyl). Strain 4G-K13T contained phosphatidylethanolamine, four unidentified phospholipids, four glycolipids, two unidentified aminolipids and two unknown lipids, while strain 4Y35T had phosphatidylethanolamine, three unidentified phospholipids, two glycolipids, five unidentified aminolipids and one unknown polar lipid. The DNA G+C contents of 4G-K13T and 4Y35T were 60.5 and 55.8 mol%, respectively. Based on all these phylogenetic, physiological and chemotaxonomic data, we suggest that strains 4G-K13T and 4Y35T represent two novel species of two novel genera in the family Acidobacteriaceae, for which the names Paracidobacterium acidisoli gen. nov., sp. nov. (type strain: 4G-K13T=GDMCC 1.1195T=NBRC 113249T) and Alloacidobacterium dinghuense gen. nov., sp. nov. (type strain: 4Y35T=KACC 21728T=NBRC 114261T) are proposed. We also propose to reclassify Acidobacterium ailaaui and Acidipila dinghuensis as Pseudacidobacterium ailaaui gen. nov., comb. nov. and Silvibacterium dinghuense comb. nov., respectively, based mainly on the results of phylogenomic analysis.


Assuntos
Acidobacteria , Fosfatidiletanolaminas , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Florestas , Glicolipídeos , Fosfolipídeos , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Solo , Microbiologia do Solo
11.
Comput Math Methods Med ; 2022: 5936773, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35693259

RESUMO

Objective: A case-control study was adopted to investigate the efficacy and side effects of irinotecan combined with nedaplatin (NP) versus paclitaxel combined with cisplatin for locally advanced cervical cancer (CC) neoadjuvant chemotherapy (NACT) and to analyze the changes in tumor marker levels. Methods: A total of 96 patients with locally advanced CC who were treated from October 2019 to October 2021 were enrolled in our hospital as the research subjects, and their clinical data were collected for retrospective analysis and grouped according to their treatment regimens. Among them, 53 patients received paclitaxel combined with cisplatin as the control group, and the other 43 patients received irinotecan combined with NP as the observation group. The clinical effectiveness of neoadjuvant chemotherapy and alterations in tumor markers (CEA, AFP, CA125, and SCCA) were compared between the two groups. The incidence of common chemotherapy side effects was observed and compared between the two groups, including nausea and vomiting, abdominal pain and diarrhea, liver function impairment, bone marrow suppression, transient hyperglycemia, rash, ECG abnormalities, peripheral neurotoxicity, and muscle aches and pains. Results: The clinical efficiency of neoadjuvant chemotherapy was 97.67% in the observation group and 81.13% in the control group, with no statistically significant difference between the groups (P > 0.05). There was no significant difference in CEA, AFP, and CA125 between the two groups before and after chemotherapy, but the decrease of SCCA before and after chemotherapy was statistically significant. There was no significant difference in the incidence of liver function damage, myelosuppression, abnormal ECG, and rash between the two groups (P > 0.05). There are statistically significant differences in the incidence of nausea and vomiting, transient hyperglycemia, peripheral neurotoxicity, and muscle aches between the observation and control groups (P < 0.05). The incidence of nausea and vomiting, transient hyperglycemia, peripheral neurotoxicity, and muscle aches was higher in the control group than in the observation group, with statistically significant differences (P < 0.05). The difference in the incidence of diarrhea and abdominal pain between the observation group and the control group was statistically significant (P < 0.05), and the incidence of diarrhea and abdominal pain in the observation group was higher than that in the control group. Conclusion: Irinotecan in combination with nedaplatin can be an effective neoadjuvant chemotherapy regimen for advanced localized cervical cancer, particularly in patients with combined diabetes.


Assuntos
Exantema , Hiperglicemia , Neoplasias do Colo do Útero , Dor Abdominal/tratamento farmacológico , Dor Abdominal/etiologia , Dor Abdominal/patologia , Protocolos de Quimioterapia Combinada Antineoplásica/efeitos adversos , Biomarcadores Tumorais , Antígeno Carcinoembrionário , Estudos de Casos e Controles , Quimioterapia Adjuvante/efeitos adversos , Cisplatino/efeitos adversos , Diarreia/tratamento farmacológico , Diarreia/etiologia , Diarreia/patologia , Exantema/induzido quimicamente , Exantema/tratamento farmacológico , Exantema/patologia , Feminino , Humanos , Hiperglicemia/tratamento farmacológico , Hiperglicemia/etiologia , Hiperglicemia/patologia , Irinotecano/efeitos adversos , Náusea/induzido quimicamente , Náusea/tratamento farmacológico , Náusea/patologia , Terapia Neoadjuvante/efeitos adversos , Estadiamento de Neoplasias , Compostos Organoplatínicos , Paclitaxel/efeitos adversos , Estudos Retrospectivos , Neoplasias do Colo do Útero/tratamento farmacológico , Neoplasias do Colo do Útero/patologia , Vômito/induzido quimicamente , Vômito/tratamento farmacológico , Vômito/patologia , alfa-Fetoproteínas/uso terapêutico
12.
Mol Cancer ; 21(1): 118, 2022 05 26.
Artigo em Inglês | MEDLINE | ID: mdl-35619132

RESUMO

BACKGROUND: PIK3CA mutation and PTEN suppression lead to tumorigenesis and drug resistance in colorectal cancer (CRC). There is no research on the role of circular RNAs (circRNAs) in regulating PIK3CA mutation and MEK inhibitor resistance in CRC. METHODS: The expression of circLHFPL2 in PIK3CA-mutant and wild-type cells and tissues was quantified by RNA-sequencing and qRT-PCR. CCK-8 assay and colony formation assay were used to evaluate cell viability. Annexin V/PI staining was implemented to assess cell apoptosis. Luciferase assay, biotin-coupled microRNA capture, and RIP assay were used to validate the interaction among potential targets. Western blotting and qRT-PCR assays were used to evaluate the expression of involved targets. Xenograft tumor in a nude mouse model was used to explore the role of circRNAs in vivo. RESULTS: RNA sequencing defined downregulated expression of circLHFPL2 in both PIK3CAH1047R (HCT116) and PIK3CAE545K (DLD1) cells. CircLHFPL2 was also downregulated in PIK3CA-mutant CRC primary cells and tissues, which was correlated with poor prognosis. CircLHFPL2 was mainly localized in the cytoplasm and its downregulation was attributed to the PI3K/AKT signaling pathway activated by phosphorylating Foxo3a. CircLHFPL2 inhibited PI3KCA-Mut CRC progression both in vitro and in vivo. Furthermore, our work indicated that circLHFPL2 acts as a ceRNA to sponge miR-556-5p and miR-1322 in CRC cells and in turn modulate the expression of PTEN. Importantly, circLHFPL2 was able to overcome PIK3CA-mediated MEK inhibitor resistance in CRC cells. CONCLUSIONS: Downregulation of circLHFPL2 sustains the activation of the PI3K/AKT signaling pathway via a positive feedback loop in PIK3CA-mutant CRC. In addition, downregulation of circLHFPL2 leads to MEK inhibitor resistance in CRC. Therefore, targeting circLHFPL2 could be an effective approach for the treatment of CRC patients harboring oncogenic PIK3CA mutations.


Assuntos
Neoplasias Colorretais , MicroRNAs , Animais , Carcinogênese , Linhagem Celular Tumoral , Classe I de Fosfatidilinositol 3-Quinases/genética , Neoplasias Colorretais/patologia , Regulação para Baixo , Humanos , Camundongos , MicroRNAs/genética , MicroRNAs/metabolismo , Quinases de Proteína Quinase Ativadas por Mitógeno/genética , Quinases de Proteína Quinase Ativadas por Mitógeno/metabolismo , Quinases de Proteína Quinase Ativadas por Mitógeno/uso terapêutico , PTEN Fosfo-Hidrolase/genética , PTEN Fosfo-Hidrolase/metabolismo , Fosfatidilinositol 3-Quinases/genética , Fosfatidilinositol 3-Quinases/metabolismo , Inibidores de Proteínas Quinases/uso terapêutico , Proteínas Proto-Oncogênicas c-akt/genética , Proteínas Proto-Oncogênicas c-akt/metabolismo , RNA Circular/genética
13.
Artigo em Inglês | MEDLINE | ID: mdl-35585373

RESUMO

Crops grown in areas contaminated by industrial and agricultural fluorine (F) have gained increasing attention, however F levels in different vegetables and lettuce cultivars are rarely reported. In situ-field experiment was designed to investigate the concentration, translocation, and health risks of F in 20 vegetable species and 25 lettuce cultivars. After the growth of 150 d for vegetables and 60 d for lettuce, F concentration (12.83-138.07 mg kg-1), translocation factor (0.16-6.32), and bio-concentration factor (1.90-13.73) varied significantly between vegetable species and lettuce cultivars. According to the hazard quotient values (based on the reference dose of F), all the vegetable species appears to pose no risk to human health, while 60% of the lettuce cultivars present potential health risks to children. Therefore, the limit value of F in vegetables for adults and children should be enacted in the future. Moreover, cabbage, green radish, spinach, leaf mustard, and Frisee lettuce (Huayu) were considered as a safe dietary product. These findings contributed to the safe cultivation of vegetables and the control of fluorosis in the areas contaminated by industrial and agricultural activities.

14.
Environ Sci Pollut Res Int ; 29(47): 71810-71825, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35604595

RESUMO

Organic and inorganic mixtures can be developed as immobilizing agents that could reduce heavy metal accumulation in crops and contribute to food safety. Here, inorganic materials (lime, L; zeolite, Z; and sepiolite, S) and organic materials (biochar, B, and compost, C) were selectively mixed to produce six composite soil amendments (LZBC, LSBC, LZC, LZB, LSC, and LSB). Given the fact that LZBC showed the best performance in decreasing soil Cd availability in the incubation experiment, it was further applied in the field condition with 14 vegetables as the test crops to investigate its effects on crop safety production in polluted greenhouse. The results showed that LZBC addition elevated rhizosphere soil pH by 0.1-2.0 units and reduced soil Cd availability by 1.85-37.99%. Both the biomass and the yields of edible parts of all vegetables were improved by LZBC addition. However, LZBC addition differently affected Cd accumulation in edible parts of the experimental vegetables, with the observation that Cd contents were significantly reduced in Allium fistulosum L., Amaranthus tricolor L., and Coriandrum sativum Linn., but increased in the three species of Lactuca sativa. Further health risk assessment showed that LZBC application significantly decreased daily intake of metal (DIM), health risk index (HRI), and target hazard quotient (THQ) for Cd in Allium fistulosum L., Amaranthus tricolor L., and Coriandrum sativum Linn., whereas increased all the indexes in Lactuca sativa. Our results showed that the effect of a composite amendment on Cd accumulation in different vegetables could be divergent and species-dependent, which suggested that it is essential to conduct a pre-experiment to verify applicable species for a specific soil amendment designed for heavy metal immobilization.


Assuntos
Metais Pesados , Oryza , Poluentes do Solo , Zeolitas , Cádmio/análise , Metais Pesados/análise , Solo/química , Poluentes do Solo/análise , Verduras
15.
Comput Human Behav ; 133: 107295, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35431427

RESUMO

Misinformation has become prevalent since the beginning of the COVID-19 pandemic. To understand why people believe and share misinformation, we conducted a nationwide survey during the COVID-19 outbreak in China. We found the indirect effects of COVID-19 risk on people's information accuracy judgment and associated information sharing intention through people's emotional states. People faced with a higher level of COVID-19 risk (measured by a 7-day moving average of daily new deaths or new cases) experienced weaker positive and stronger negative emotions, and heightened emotionality (both the positive and negative emotions) was associated with increased belief in and greater likelihood to share the COVID-19 information regardless of veracity. We also found that only the negative emotion mediated the relation between the COVID-19 risk and the truth discernment regarding accuracy judgment. However, the mediating effect of negative emotion disappeared among people with high analytic thinking ability. These findings suggest that the analytic thinking ability could moderate the destructive relationship between negative emotion and accuracy discernment. Based on a large sample, our findings provide actionable insights for the policymakers to respond to the spread of misinformation appropriately and promptly during the pandemic.

16.
Clin Transl Oncol ; 24(8): 1631-1642, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35303268

RESUMO

PURPOSE: To explore the regulatory effect of let-7a-5p/TGFBR1/Smad3 on the proliferation activity of cervical cancer cells. METHODS: The difference in let-7a-5p expression between normal people and patients with cervical cancer was detected by miREIA assay. The differences of let-7a-5p expression between cervical cancer cell line C33a and adjacent normal epithelial cell line HUCEC were determined by qRT-PCR. RESULTS: miREIA result showed that let-7a-5p concentrations were 178.5 ± 24.3 µg/L in healthy individuals and 106.1 ± 14.8 µg/L in cervical cancer patients (P = 0.0002). qRT-PCR showed that let-7a-5p in cervical cancer tissue (0.57 ± 0.03) was lower than that in adjacent normal tissue (0.84 ± 0.04, P = 0.0107). Compared with normal cervical epithelial cells (HUCEC), the expression of let-7a-5p was lower in cervical cancer cells (C33a, Hela, P = 0.0001). The results of CCK-8 and EDU detection showed that activation of let-7a-5p inhibited the proliferation of C33a (P = 0.00130, P << 0.0001) and Hela (P = 0.00254, P = 0.0066) cells. According to the analysis using Starbase V2.0 online database, let-7a-5p could target TGFßR1 in cervical cancer cell lines, and the let-7a-5p mimic reduces the mRNA expression level of TGFßR1 in cervical cancer cell C33a (P = 0.0067). Western blot results showed that TGFBR1 expression significantly decreased in cervical cancer cells after let-7a-5p mimic treatment (P = 0.0048) and significantly increased after let-7a-5p mimic inhibitor treatment (P = 0.0003). CONCLUSIONS: let-7a-5p represents the independent novel anti-oncogenes in cervical cancer, which can regulate TGF-ß1/TGFBR1/pSmad3 cell pathway and interfere with the proliferation of cervical cancer cells. Therefore, let-7a-5p can serve as a novel potential therapeutic target for the treatment of cervical cancer.


Assuntos
MicroRNAs , Neoplasias do Colo do Útero , Linhagem Celular Tumoral , Proliferação de Células , Feminino , Humanos , MicroRNAs/genética , RNA Mensageiro , Receptor do Fator de Crescimento Transformador beta Tipo I/genética , Proteína Smad3/genética , Neoplasias do Colo do Útero/genética
17.
Int J Gen Med ; 15: 2377-2387, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35264875

RESUMO

Objective: The aim of this study is to determine if cervical cerclage administration reduces the preterm birth (PTB) rate at a gestational age (GA) of 16-28 weeks in women with twin pregnancy. Methods: This is a retrospective cohort study on asymptomatic twin pregnancy with an ultrasound-identified cervix length (CL) of ≦25 mm. The patients were divided into two groups: ultrasound-indicated cerclage (UIC) group and control (expectant management) group. The primary outcome was a PTB rate at <34 weeks. A logistic regression was also performed, and a subgroup analysis stratified by CL and GA at first short cervix diagnosis was planned. Results: In all 320 women, there were no differences in the overall <34-week PTB rates and neonatal outcomes between the UIC group and control group. After performing a multivariate logistic regression analysis, the subgroup analyses were planned. In patients with a CL of ≦15 mm, the <34-week PTB rate was significantly decreased in the UIC subgroup compared with the control subgroup (60.78% vs 78.26%; odds ratio (OR) = 0.43, confidence interval (CI) = 95% [0.22-0.86]; and p = 0.020). In patients with a first short cervix diagnosis GA of ≦24 weeks, the <34-week PTB rate was significantly decreased in the UIC subgroup when compared with the control subgroup (61.54% vs 84.75%; OR = 0.29; CI = 95% [0.13-0.63]; and p = 0.001). Furthermore, compared with the control groups, the UIC groups had higher mean birth weight, lower perinatal mortality, and lower NICU admission, and the differences were statistically significant. Conclusion: UIC could significantly reduce the <34-week PTB rate and improve perinatal outcomes in patients with a CL of ≦15mm or first short cervix diagnosis GA of ≦24 weeks with asymptomatic twin pregnancy during the second trimester.

18.
Environ Pollut ; 293: 118510, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34793909

RESUMO

Plant growth regulators (PGRs) assisted phytoextraction was investigated as a viable phytoremediation technology to increase the phytoextraction efficiency in contaminated soils. This study aimed to evaluate the cadimum (Cd)/lead (Pb)/zinc (Zn) phytoextraction efficiency by a hyperaccumulator Sedum alfredii Hance (S. alfredii) treated with 9 PGRs, including indole-3-acetic acid (IAA), gibberellin (GA3), cytokinin (CKs), abscisic acid (ABA), ethylene (ETH), brassinosteroid (BR), salicylic acid (SA), strigolactones (SL) and jasmonic acid (JA), in slightly or heavily contaminated (SC and HC, respectively) soil. Results demonstrated that PGRs were able to improve S. alfredii biomass, the most significant increases were observed in GA3 and SL for HC soil, while for SC soil, IAA and BR exhibited positive effects. The levels of Cd, Pb and Zn in the shoots of S. alfredii treated with ABA and SL were noticeably greater than in the CK treatment in HC soil, while the uptake of metals were increased by IAA and CKs in SC soil. Combined with the results of biomass and metal contents in shoots, we found that ABA showed the highest Cd removal efficiency and the maximum Pb and Zn removal efficiency was observed with GA3, which was 62.99%, 269.23%, and 41.18%, respectively higher than the control in HC soil. Meanwhile, compared to control, the maximum removal efficiency of Cd by IAA treatment (52.80%), Pb by JA treatment (165.1%), and Zn by BR treatment (44.97%) in the SC soil. Overall, our results suggested that these PGRs, especially, ABA, SL, IAA, BR and GA3 had great potential in improving phytoremediation efficiency of S. alfredii grown in contaminated soils.


Assuntos
Metais Pesados , Sedum , Poluentes do Solo , Biodegradação Ambiental , Cádmio/análise , Chumbo , Metais Pesados/análise , Reguladores de Crescimento de Plantas , Solo , Poluentes do Solo/análise , Zinco/análise
19.
J Health Psychol ; 27(7): 1646-1658, 2022 06.
Artigo em Inglês | MEDLINE | ID: mdl-33752460

RESUMO

This study found that personal wisdom was correlated positively with Chinese older adults' quality of life regardless of their place of residence (rural vs urban). Both self-esteem and depression were found to account directly for the relation between personal wisdom and quality of life among the urban, but not the rural residents. The findings overall highlighted the importance of considering personal wisdom as a beneficial psychological resource that helps older adults maintain a high quality of life in old age. Further, the rural-urban difference indicates the need for future personal wisdom studies on low-income and less educated older populations.


Assuntos
Qualidade de Vida , População Rural , Idoso , China , Humanos , População Urbana
20.
Front Cell Dev Biol ; 10: 1035033, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36589752

RESUMO

Objective: This study aimed to identify ROS1 fusion partners in Chinese patients with solid tumors. Methods: Next-generation sequencing (NGS) analysis was used to detect ROS1 rearrangement in 45,438 Chinese patients with solid tumors between 2015 and 2020, and the clinical characteristics and genetic features of gene fusion were evaluated. H&E staining of the excised tumor tissues was conducted. Samples with a tumor cell content ≥ 20% were included for subsequent DNA extraction and sequencing analysis. Results: A total of 92 patients with ROS1 rearrangements were identified using next-generation sequencing, and the most common histological type lung cancer. From the 92 ROS1 fusion cases, 24 ROS1 fusion partners had been identified, including 14 novel partners and 10 reported partners. Of these, CD74, EZR, SDC4, and TPM3 were the four most frequently occurring partners. Fourteen novel ROS1 fusion partners were detected in 16 patients, including DCBLD1-ROS1, FRK-ROS1, and VGLL2-ROS1. In many patients, the ROS1 breakpoint was located between exons 32 and 34. Conclusion: This study describes 14 novel ROS1 fusion partners based on the largest ROS1 fusion cohort, and the ROS1 breakpoint was mostly located between exons 32 and 34. Additionally, next-generation sequencing is an optional method for identifying novel ROS1 fusions.

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