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1.
Basic Clin Androl ; 32(1): 1, 2022 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-34983365

RESUMO

BACKGROUND: The ischiocavernosus muscle (ICM) encompasses a pair of short pinnate muscles attached to the pelvic ring. The ICM begins at the ischial tuberosity and ends at the crus of the penis while covering the surface of the crus. According to the traditional view, the contraction of the ICM plays an auxiliary role in penile erection. However, we have previously shown that the ICM plays an important role in penile erection through an indirect method of diagnosing erectile dysfunction (ED) caused by ICM injury by observing the infertility of paired female rats. Since intracavernosal pressure (ICP) is the current gold standard for diagnosing ED, this study aimed to amputate unilaterally/bilaterally the ICM to establish an ED model by detecting the ICP, recording the infertility of matching female rats, and comparing the two methods. RESULTS: Forty sexually mature adult male rats were selected and randomly divided into the following groups: the control group (n = 10), sham operation group (n = 10), unilateral ischiocavernosus muscle (Uni-ICM) amputation group (n = 10), and bilateral ischiocavernosus muscle (Bi-ICM) amputation group (n = 10). Eighty female reproductive rats were randomly assigned to the above groups at a ratio of 2:1. We evaluated the time to conception for the paired female rats and the effects of unilateral/bilateral severing of the ICM on erectile function. The results showed that the baseline and maximum intracavernosal pressure (ICP) in the control group, sham operation group, Uni-ICM amputation group, and Bi-ICM amputation group were 17.44±2.50 mmHg and 93.51±10.78 mmHg, 17.81±2.81 mmHg and 95.07±10.40 mmHg, 16.73±2.11 mmHg and 83.49±12.38 mmHg, and 14.78±2.78 mmHg and 33.57±6.72 mmHg, respectively, immediately postsurgery. The max ICP in the Bi-ICM amputation group was lower than that in the remaining three groups (all P<0.05). The pregnancy rates were 100, 100, 90, and 0% in the control group, sham operation group, Uni-ICM amputation group, and the Bi-ICM amputation group, respectively. The pregnancy rate in the Bi-ICM amputation group was significantly lower than that in the remaining groups (all P<0.05). The time to conception was approximately 7-10 days later in the Uni-ICM amputation group than in the control and sham groups (all P<0.05). CONCLUSIONS: Male rats undergoing Bi-ICM amputation may develop permanent ED, which affects their fertility. In contrast, rats undergoing Uni-ICM amputation may experience transient ED.


RéSUMé: INTRODUCTION: Le muscle Ischiocavernosus (MIC) est une paire de muscles courts attachés à l'anneau pelvien. Elle commence à la tubérosité ischiale et se termine au crus du pénis tout en couvrant la surface de ce dernier. Selon la vision traditionnelle, la contraction du MIC joue un rôle auxiliaire dans l'érection pénienne. Cependant, nos travaux précédents ont montré qu'il joue un rôle important dans l'érection pénienne par une méthode indirecte de diagnostic de la dysfonction érectile (DE) induite par une blessure du MIC en observant l'infertilité des rats femelles appariés. Comme la pression intracaverneuse (PIC) est actuellement l'étalon de référence pour le diagnostic, cette étude visait à amputer unilatéralement/bilatéralement l'ICM pour établir un modèle de DE par détection de l'ICP, par détection de l'infertilité de rats femelles appariés, et par comparaison des deux méthodes. RéSULTATS: Quarante rats mâles adultes sexuellement matures ont été sélectionnés et répartis aléatoirement dansquatre groupes groupe témoin (n = 10), groupe d'opération simulée (n = 10), groupe d'amputation unilatérale du MIC (Uni-MIC) (n = 10), et groupe d'amputation bilatérale du MIC (Bi-MIC) (n = 10). Quatre-vingts rats femelles en période de reproduction ont été réparties de façon aléatoire dans chacun des groupes mentionnés ci-dessus, selon un rapport de 2:1. Nous avons évalué le temps avant la conception des rats femelles appariés et les effets de l'amputation Uni /Bi-MIC sur la fonction érectile. Les résultats ont montré que la pression de base et la PIC maximale dans le groupe témoin, le groupe d'opération simulée, le groupe Uni-MIC et le groupe Bi-MIC étaient respectivement de 17.44 ± 2.50 mmHg et 93.51 ± 10.78 mmHg, 17.81 ± 2.81 mmHg et 95.07 ± 10.40 mmHg, 16.73 ± 2.11 mmHg et 83.49 ± 12.38 mmHg, et 14.78 ± 2.78 mmHg et 33.57 ± 6.72 mmHg, immédiatement après l'opération. La PIC maximale dans le groupe Bi-MIC était inférieure à celles des trois autres groupes (tous P < 0.05). Les taux de grossesse étaient de 90% dans le groupe Uni-MIC et de 0% dans le groupe Bi-MIC. Le taux de grossesse dans le groupe Bi-MIC était significativement plus faible que dans les autres groupes (tous P < 0.05). Le temps de conception a été d'environ 7 à 10 jours plus tard dans le groupe Uni-MIC que dans le groupe témoin et le groupe d'opération simulée (tous P < 0.05). CONCLUSIONS: Les rats mâles subissant une amputation Bi-MIC peuvent développer une DE permanente qui affecte leur fertilité. En revanche, ceux qui subissent une amputation Uni-MIC peuvent connaître des troubles érectiles transitoires.

2.
Nat Commun ; 13(1): 442, 2022 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-35064113

RESUMO

Manufacturing molecule-based functional elements directly at device interfaces is a frontier in bottom-up materials engineering. A longstanding challenge in the field is the covalent stabilization of pre-assembled molecular architectures to afford nanodevice components. Here, we employ the controlled supramolecular self-assembly of anthracene derivatives on a hexagonal boron nitride sheet, to generate nanographene wires through photo-crosslinking and thermal annealing. Specifically, we demonstrate µm-long nanowires with an average width of 200 nm, electrical conductivities of 106 S m-1 and breakdown current densities of 1011 A m-2. Joint experiments and simulations reveal that hierarchical self-assembly promotes their formation and functional properties. Our approach demonstrates the feasibility of combined bottom-up supramolecular templating and top-down manufacturing protocols for graphene nanomaterials and interconnects, towards integrated carbon nanodevices.

3.
Materials (Basel) ; 15(2)2022 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-35057238

RESUMO

This paper investigates the compression behavior and failure criteria of lightweight aggregate concrete (LAC) under triaxial loading. A total of 156 specimens were tested for three parameters: concrete strength, lateral confining pressure and aggregate immersion time, and their effects on the failure mode of LAC and the triaxial stress-strain relationship of LAC is studied. The research indicated that, as the lateral constraint of the specimen increases, the failure patterns change from vertical splitting failure to oblique shearing failure and then to indistinct traces of damage. The stress-strain curve of LAC specimens has an obvious stress plateau, and the curve no longer appears downward when the confining pressure exceeds 12 MPa. According to the experimental phenomenon and test data, the failure criterion was examined on the Mohr-Coulomb theory, octahedral shear stress theory and Rendulic plane stress theory, which well reflects the behavior of LAC under triaxial compression. For the convenience of analysis and application, the stress-strain constitutive models of LAC under triaxial compression are recommended, and these models correlate well with the test results.

4.
Sci Rep ; 11(1): 14122, 2021 07 08.
Artigo em Inglês | MEDLINE | ID: mdl-34239023

RESUMO

Total testosterone levels decline with age, while prostate volume and the prevalence of benign prostatic hyperplasia increase with age. We sought to investigate the correlation of serum testosterone levels with prostate volume in aging men. We analyzed clinical data obtained from 416 ostensibly healthy men who underwent routine health check-ups and recruited and collected data from these subjects 4 years later. We analyzed the correlation between prostate volume and relevant factors, as well as the correlation between changes in prostate volume and low testosterone over a 4-year period. Men with low testosterone had significantly larger prostate volume than those in the normal testosterone group (26.86 ± 8.75 vs. 24.06 ± 6.77 P = 0.02), and subjects with low testosterone had significantly higher levels of obesity-related factors, including waist circumference, body mass index, and insulin (all P < 0.001). After adjustment for age, testosterone level was negatively correlated with prostate volume (P = 0.004), and prostate volume and 4-year changes in prostate volume were associated with low testosterone. With increased testosterone level, prostate volume showed a significant linear decreasing trend. These findings provide evidence of the relationship between testosterone and prostate volume. Additional large studies are needed to confirm these preliminary results.


Assuntos
Envelhecimento/sangue , Próstata/anatomia & histologia , Testosterona/sangue , Idoso , Humanos , Masculino , Pessoa de Meia-Idade , Tamanho do Órgão , Análise de Regressão
5.
Adv Mater ; 33(29): e2006836, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34096113

RESUMO

Macromolecular films are crucial functional materials widely used in the fields of mechanics, electronics, optoelectronics, and biology, due to their superior properties of chemical stability, small density, high flexibility, and solution-processing ability. Their electronic and mechanical properties, however, are typically much lower than those of crystalline materials, as the macromolecular films have no long-range structural ordering. The state-of-the-art for producing highly ordered macromolecular films is still facing a great challenge due to the complex interactions between adjacent macromolecules. Here, the growth of textured macromolecular films on a designed graphene/high-index copper (Cu) surface is demonstrated. This successful growth is driven by a patterned potential that originates from the different amounts of charge transfer between the graphene and Cu surfaces with, alternately, terraces and step edges. The textured films exhibit a remarkable improvement in remnant ferroelectric polarization and fracture strength. It is also demonstrated that this growth mechanism is universal for different macromolecules. As meter-scale graphene/high-index Cu substrates have recently become available, the results open a new regime for the production and applications of highly ordered macromolecular films with obvious merits of high production and low cost.

6.
Chem Commun (Camb) ; 57(49): 6031-6034, 2021 Jun 17.
Artigo em Inglês | MEDLINE | ID: mdl-34032226

RESUMO

Graphene nanoribbons (GNRs), quasi-one-dimensional strips of graphene, exhibit a nonzero bandgap due to quantum confinement and edge effects. In the past decade, different types of GNRs with atomically precise structures have been synthesized by a bottom-up approach and have attracted attention as a novel class of semiconducting materials for applications in electronics and optoelectronics. We report the large-scale, inexpensive growth of high-quality oxygen-boron-oxygen-doped chiral GNRs with a defined structure using chemical vapor deposition. For the first time, a regular 2D self-assembly of such GNRs has been demonstrated, which results in a unique orthogonal network of GNRs. Stable and large-area GNR films with an optical bandgap of ∼1.9 eV were successfully transferred onto insulating substrates. This ordered network structure of semiconducting GNRs holds promise for controlled device integration.

7.
Biomed Res Int ; 2021: 8862282, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33542928

RESUMO

PURPOSE: To evaluate the clinical efficacy of prodom in the administration of urokinase in the vagina in couples with impaired semen liquefaction. MATERIALS AND METHODS: Overall, 261 patients with impaired semen liquefaction were randomly divided into prodom-assisted urokinase treatment (PAUT) group (n = 91), syringe-assisted urokinase treatment (SAUT) group (n = 86), and traditional treatment (TT) group (n = 84) in the first stage. If the first stage of treatment failed, other treatment methods were initiated instead and the patients were grouped according to the newer treatment method in the second stage. The pregnancy rate, time-to-conception, and treatment costs were evaluated in each group. RESULTS: In the first stage, the pregnancy rate in the PAUT, SAUT, and TT groups was 69.23%, 29.07%, and 22.62%, respectively; the time-to-conception was 2.66 ± 1.44, 3.69 ± 2.61, and 3.86 ± 3.00 months, respectively; the treatment costs were 658.18 ± 398.40, 666.67 ± 507.50, and 680.56 ± 480.94 $, respectively. The pregnancy rate and time-to-conception were different in the PAUT group compared with those in SAUT and TT groups (all P < 0.05). However, the difference in treatment costs was not significant (P = 0.717). In the second stage, 154 nonpregnant patients were divided into nine treatment groups, and the effects of changing TT to PAUT on the pregnancy rate, time-to-conception, and treatment costs were observed to be different from those of other treatments (all P < 0.05). CONCLUSION: Prodom-assisted urokinase can effectively treat male infertility secondary to impaired semen liquefaction.


Assuntos
Infertilidade Masculina/tratamento farmacológico , Sêmen/efeitos dos fármacos , Motilidade Espermática/fisiologia , Ativador de Plasminogênio Tipo Uroquinase/administração & dosagem , Adulto , Feminino , Humanos , Infertilidade Masculina/metabolismo , Infertilidade Masculina/patologia , Masculino , Pessoa de Meia-Idade , Gravidez , Taxa de Gravidez , Sêmen/química , Sêmen/fisiologia , Contagem de Espermatozoides , Vagina/fisiologia , Adulto Jovem
8.
Sci Adv ; 7(9)2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33637529

RESUMO

Van der Waals heterostructures consisting of graphene and transition metal dichalcogenides have shown great promise for optoelectronic applications. However, an in-depth understanding of the critical processes for device operation, namely, interfacial charge transfer (CT) and recombination, has so far remained elusive. Here, we investigate these processes in graphene-WS2 heterostructures by complementarily probing the ultrafast terahertz photoconductivity in graphene and the transient absorption dynamics in WS2 following photoexcitation. We observe that separated charges in the heterostructure following CT live extremely long: beyond 1 ns, in contrast to ~1 ps charge separation reported in previous studies. This leads to efficient photogating of graphene. Furthermore, for the CT process across graphene-WS2 interfaces, we find that it occurs via photo-thermionic emission for sub-A-exciton excitations and direct hole transfer from WS2 to the valence band of graphene for above-A-exciton excitations. These findings provide insights to further optimize the performance of optoelectronic devices, in particular photodetection.

9.
J Am Chem Soc ; 142(42): 17881-17886, 2020 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-33021787

RESUMO

Bottom-up-synthesized graphene nanoribbons (GNRs) with excellent electronic properties are promising materials for energy storage systems. Herein, we report bottom-up-synthesized GNR films employed as electrode materials for micro-supercapacitors (MSCs). The micro-device delivers an excellent volumetric capacitance and an ultra-high power density. The electrochemical performance of MSCs could be correlated with the charge carrier mobility within the differently employed GNRs, as determined by pump-probe terahertz spectroscopy studies.

10.
Adv Mater ; 32(45): e2001893, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32945038

RESUMO

Graphene nanoribbons (GNRs) are quasi-1D graphene strips, which have attracted attention as a novel class of semiconducting materials for various applications in electronics and optoelectronics. GNRs exhibit unique electronic and optical properties, which sensitively depend on their chemical structures, especially the width and edge configuration. Therefore, precision synthesis of GNRs with chemically defined structures is crucial for their fundamental studies as well as device applications. In contrast to top-down methods, bottom-up chemical synthesis using tailor-made molecular precursors can achieve atomically precise GNRs. Here, the synthesis of GNRs on metal surfaces under ultrahigh vacuum (UHV) and chemical vapor deposition (CVD) conditions is the main focus, and the recent progress in the field is summarized. The UHV method leads to successful unambiguous visualization of atomically precise structures of various GNRs with different edge configurations. The CVD protocol, in contrast, achieves simpler and industry-viable fabrication of GNRs, allowing for the scale up and efficient integration of the as-grown GNRs into devices. The recent updates in device studies are also addressed using GNRs synthesized by both the UHV method and CVD, mainly for transistor applications. Furthermore, views on the next steps and challenges in the field of on-surface synthesized GNRs are provided.

11.
Adv Mater ; 32(26): e2001268, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32378243

RESUMO

Van der Waals heterostructures (VDWHs), obtained via the controlled assembly of 2D atomically thin crystals, exhibit unique physicochemical properties, rendering them prototypical building blocks to explore new physics and for applications in optoelectronics. As the emerging alternatives to graphene, monolayer transition metal dichalcogenides and bottom-up synthesized graphene nanoribbons (GNRs) are promising candidates for overcoming the shortcomings of graphene, such as the absence of a bandgap in its electronic structure, which is essential in optoelectronics. Herein, VDWHs comprising GNRs onto monolayer MoS2 are fabricated. Field-effect transistors (FETs) based on such VDWHs show an efficient suppression of the persistent photoconductivity typical of MoS2 , resulting from the interfacial charge transfer process. The MoS2 -GNR FETs exhibit drastically reduced hysteresis and more stable behavior in the transfer characteristics, which is a prerequisite for the further photomodulation of charge transport behavior within the MoS2 -GNR VDWHs. The physisorption of photochromic molecules onto the MoS2 -GNR VDWHs enables reversible light-driven control over charge transport. In particular, the drain current of the MoS2 -GNR FET can be photomodulated by 52%, without displaying significant fatigue over at least 10 cycles. Moreover, four distinguishable output current levels can be achieved, demonstrating the great potential of MoS2 -GNR VDWHs for multilevel memory devices.

12.
Prostate ; 80(6): 481-490, 2020 05.
Artigo em Inglês | MEDLINE | ID: mdl-32104919

RESUMO

OBJECTIVE: To investigate the potential mechanism of the effect of metabolic syndrome (MetS) on prostate volume (PV) and the risk of benign prostatic hyperplasia (BPH)/lower urinary tract symptoms (LUTS) and the relationships of MetS and the major pathogenic factors of MetS with the clinical progression of BPH/LUTS in older Chinese men. SUBJECTS AND METHODS: We analyzed clinical data obtained from 506 ostensibly healthy men who underwent routine health check-ups and recruited 415 subjects from a group of previously studied men after 4 years. We evaluated the associations of major pathological factors of MetS, including insulin resistance, subclinical inflammatory state, and sex hormone changes, with PV, the risk of BPH and the clinical progression of BPH/LUTS by using multiple linear regression and logistic regression. RESULTS: After adjustment for age, insulin, HOMA (homeostatic model assessment) index, leptin, resistin, adiponectin, C-reactive protein, tumor necrosis factor-α (TNF-α), sex hormone-binding globulin, and testosterone levels were significantly associated with PV (all P < .05), and in the age-adjusted logistic regression model, positive associations of resistin and TNF-α with BPH/LUTS were found (OR, 1.662, P = .007 and OR, 1.044, P < .001, respectively). Predictors of BPH/LUTS clinical progression were significantly correlated with MetS and TNF-α. The group with higher TNF-α levels had a higher rate of newly diagnosed BPH (9.5% vs 19.1%, P = .006) and a greater increase in PV levels (0.61 ± 0.08 vs 1.09 ± 0.35 cm3 , P <.001) after 4 years. CONCLUSIONS: MetS and its pathological factors were associated with an increased PV and an increased risk of BPH/LUTS that is more prone to clinical progression. TNF-α may serve as an early biological indicator to identify which patients with BPH/LUTS are at higher risk of unfavorable outcomes.


Assuntos
Síndrome Metabólica/metabolismo , Síndrome Metabólica/patologia , Hiperplasia Prostática/metabolismo , Hiperplasia Prostática/patologia , Idoso , Pressão Sanguínea/fisiologia , Humanos , Modelos Logísticos , Masculino , Próstata/metabolismo , Próstata/patologia , Circunferência da Cintura/fisiologia
13.
Phys Chem Chem Phys ; 22(10): 5667-5672, 2020 Mar 14.
Artigo em Inglês | MEDLINE | ID: mdl-32103224

RESUMO

Hysteresis in the current response to a varying gate voltage is a common spurious effect in carbon-based field effect transistors. Here, we use electric transport measurements to probe the charge transport in networks of armchair graphene nanoribbons with a width of either 5 or 9 carbon atoms, synthesized in a bottom-up approach using chemical vapor deposition. Our systematic study on the hysteresis of such graphene nanoribbon transistors, in conjunction with temperature-dependent transport measurements shows that the hysteresis can be fully accounted for by trapping/detrapping carriers in the SiO2 layer. We extract the trap densities and depth, allowing us to identify shallow traps as the main origin of the hysteresis effect.

14.
Sci Rep ; 10(1): 1988, 2020 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-32029795

RESUMO

In graphene nanoribbons (GNRs), the lateral confinement of charge carriers opens a band gap, the key feature that enables novel graphene-based electronics. Despite great progress, reliable and reproducible fabrication of single-ribbon field-effect transistors (FETs) is still a challenge, impeding the understanding of the charge transport. Here, we present reproducible fabrication of armchair GNR-FETs based on networks of nanoribbons and analyze the charge transport mechanism using nine-atom wide and, in particular, five-atom-wide GNRs with large conductivity. We show formation of reliable Ohmic contacts and a yield of functional FETs close to unity by lamination of GNRs to electrodes. Modeling the charge transport in the networks reveals that transport is governed by inter-ribbon hopping mediated by nuclear tunneling, with a hopping length comparable to the physical GNR length. Overcoming the challenge of low-yield single-ribbon transistors by the networks and identifying the corresponding charge transport mechanism is a key step forward for functionalization of GNRs.

15.
Materials (Basel) ; 13(3)2020 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-32041205

RESUMO

To study the seismic performance of embedded polyvinyl chloride (PVC) pipe confined reinforced high-strength concrete (PVC-RHC) columns, five specimens are designed for cyclic loading test, which include three PVC-RHC column specimens, an embedded circle steel tube confined reinforced high-strength concrete (CST-RHC) column specimen, and a reinforced high-strength concrete (RHC) column specimen. The failure mechanism and morphology are revealed by experiments. The influences of PVC pipe diameter, axial compression ratio, and concrete strength on seismic performance indexes are analyzed. The research results indicate thhe following: all specimens displayed shear baroclinic failure. Compared with RHC specimens, the hysteretic curves of the PVC-RHC specimen and CST-RHC specimen were fuller; furthermore, their energy dissipation capacity, deformation, and ductility were more beneficial. With the increase of the diameter-length ratio and axial pressure, the energy dissipation capacity and deformation capacity of PVC-RHC specimens decreased. The shear bearing capacity of the PVC-RHC specimen calculated with "concrete structure design code" (GB 50010-2010) was smaller than the test results by 25%, showing an excessive safety margin. Thus, according to the failure mechanism of the PVC-RHC specimen, a new calculation formula of shear bearing capacity is deduced, which is in good agreement with the experimental results.

16.
OTA Int ; 3(4): e081, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33937711

RESUMO

OBJECTIVE: To explore the correlation between ischiocavernosus muscle injury (ICMI) with different types of pelvic fractures and erectile dysfunction (ED) after pelvic fracture. DESIGN: Retrospective analysis of a prospective database. SETTING: The study was carried out at the affiliated hospital of Zunyi Medical University. PATIENTS/PARTICIPANTS: A total of 776 male patients with pelvic fracture, aged 18 to 67 years, were recruited for this study by retrospective analysis, and based on the diagnosis of ED and the presence of ICMI, the participants were divided into ED and non-ED groups as well as ICMI and non-ICMI groups. INTERVENTION: No. MAIN OUTCOME MEASUREMENTS: ICMI, the type of pelvic fracture, International Index of Erectile Function-5 scores. Computed tomography/magnetic resonance imaging scans, electromyography (motor unit potential) was used to diagnose ICMI. RESULTS: The International Index of Erectile Function-5 score was 19.7 ±â€Š5.9. The incidence of ED was 27.3%, the duration time of ED was 30 ±â€Š23 months, and the incidence of reversible ED was 39.6% and of irreversible ED was 60.4%. The incidence of ICMI was 29.4%, among which the incidence of unilateral injury was 57.9%, and the incidence of bilateral injury was 42.1%. Among all pelvic fractures, the incidence of pubic ramus fracture was 88.1%. Bilateral pubic ramus fractures, bilateral fractures of the ischial ramus, and ICMI were independent risk factors for ED after pelvic fracture. Bilateral pubic ramus fractures and pubic symphysis separation were independent risk factors for ICMI. Unilateral ICMI was an independent risk factor for reversible ED, while bilateral ICMI was an independent risk factor for irreversible ED. CONCLUSIONS: ICMI is associated with ED and may be a cause for ED, while pubic ramus fracture, ischial ramus fracture, and pubic symphysis separation may be the main causes of ICMI. Unilateral ICMI may be the main risk factor for transient ED, and bilateral ICMI may be the main risk factor for permanent ED.

17.
Chin Med J (Engl) ; 132(24): 2941-2949, 2019 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-31855962

RESUMO

BACKGROUND: X-linked inhibitor of apoptosis (XIAP) is a vital factor in the anti-apoptosis mechanism of tumors and is highly expressed in renal cell carcinoma (RCC). However, the mechanism through which XIAP regulates DNA damage repair is unknown. This study investigated the regulatory mechanism of XIAP in etoposide-induced apoptosis in two Caki-1 cell lines with high or low XIAP expression. METHODS: The two cell lines were established using RNA interference technology. The differentially expressed proteins in the two cell lines were globally analyzed through an isobaric tags for relative and absolute quantitation-based quantitative proteomics approach. Proteomic analysis revealed 255, 375, 362, and 5 differentially expressed proteins after 0, 0.5, 3, and 12 h of drug stimulation, respectively, between the two cell lines. The identified differentially expressed proteins were involved in numerous biological processes. In addition, the expression of histone proteins (H1.4, H2AX, H3.1, H3.2, and H3.3) was drastically altered, and the effects of XIAP silencing were accompanied by the marked downregulation of H2AX. Protein-protein interactions were assessed and confirmed through immunofluorescence and Western blot analyses. RESULTS: The results suggested that XIAP may act as a vital cell signal regulator that regulates the expression of DNA repair-related proteins, such as H2AX, and influences the DNA repair process. CONCLUSIONS: Given these functions, XIAP may be the decisive factor in determining the sensitivity of RCC cell apoptosis induction in response to chemotherapeutic agents.


Assuntos
Apoptose/efeitos dos fármacos , Carcinoma de Células Renais/tratamento farmacológico , Etoposídeo/farmacologia , Histonas/análise , Neoplasias Renais/tratamento farmacológico , Proteômica/métodos , Proteínas Inibidoras de Apoptose Ligadas ao Cromossomo X/fisiologia , Carcinoma de Células Renais/química , Carcinoma de Células Renais/patologia , Linhagem Celular Tumoral , Humanos , Neoplasias Renais/química , Neoplasias Renais/patologia , Proteínas Inibidoras de Apoptose Ligadas ao Cromossomo X/análise
18.
BMC Urol ; 19(1): 119, 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31752806

RESUMO

BACKGROUND: Metabolic syndrome (MetS) and serum prostate-specific antigen (PSA) levels are correlated. To investigate the underlying effect of MetS on PSA levels, the relationship between the major pathogenic factors of MetS and serum PSA levels was studied. METHODS: A total of 506 ostensibly healthy men who underwent routine health check-ups were recruited to this study. We evaluated the effect of the major pathogenic factors of MetS, which included insulin resistance, a subclinical inflammatory state and sexual hormone changes, on serum PSA levels by using linear regression analysis and multivariate analysis after adjusting for age, BMI and prostate volume. RESULTS: When simultaneously adjusting for age, BMI, prostate volume and high-density lipoprotein cholesterol, serum insulin levels and SHBG levels were inversely correlated with serum PSA levels (P = 0.049 and P = 0.004, respectively), and testosterone levels were positively correlated with serum PSA levels (P = 0.039). In multivariate regression models, serum insulin levels and serum SHBG levels were significantly associated with serum PSA levels (both P < 0.001). CONCLUSIONS: Among the major pathogenic factors of metabolic syndrome, insulin resistance and sexual hormone changes may be the most significant contributors to the decline in serum PSA levels.


Assuntos
Síndrome Metabólica/sangue , Síndrome Metabólica/etiologia , Antígeno Prostático Específico/sangue , Idoso , Correlação de Dados , Humanos , Masculino , Pessoa de Meia-Idade , Projetos Piloto , Fatores de Risco
19.
Nano Lett ; 19(12): 9029-9036, 2019 12 11.
Artigo em Inglês | MEDLINE | ID: mdl-31742413

RESUMO

Due to its outstanding electrical properties and chemical stability, graphene finds widespread use in various electrochemical applications. Although the presence of electrolytes strongly affects its electrical conductivity, the underlying mechanism has remained elusive. Here, we employ terahertz spectroscopy as a contact-free means to investigate the impact of ubiquitous cations (Li+, Na+, K+, and Ca2+) in aqueous solution on the electronic properties of SiO2-supported graphene. We find that, without applying any external potential, cations can shift the Fermi energy of initially hole-doped graphene by ∼200 meV up to the Dirac point, thus counteracting the initial substrate-induced hole doping. Remarkably, the cation concentration and cation hydration complex size determine the kinetics and magnitude of this shift in the Fermi level. Combined with theoretical calculations, we show that the ion-induced Fermi level shift of graphene involves cationic permeation through graphene. The interfacial cations located between graphene and SiO2 electrostatically counteract the substrate-induced hole doping effect in graphene. These insights are crucial for graphene device processing and further developing graphene as an ion-sensing material.

20.
Chem Sci ; 10(4): 964-975, 2019 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-30774890

RESUMO

Graphene nanoribbons (GNRs) are quasi-one-dimensional subunits of graphene and have open bandgaps in contrast to the zero-bandgap graphene. The high potential of GNRs as a new family of carbon-based semiconductors, e.g. for nanoelectronic and optoelectronic applications, has boosted the research attempts towards fabrication of GNRs. The predominant top-down methods such as lithographical patterning of graphene and unzipping of carbon nanotubes cannot prevent defect formation. In contrast, bottom-up chemical synthesis, starting from tailor-made molecular precursors, can achieve atomically precise GNRs. In this account, we summarize our recent research progress in the bottom-up synthesis of GNRs through three different methods, namely (1) in solution, (2) on-surface under ultrahigh vacuum (UHV) conditions, and (3) on-surface through chemical vapour deposition (CVD). The solution synthesis allows fabrication of long (>600 nm) and liquid-phase-processable GNRs that can also be functionalized at the edges. On the other hand, the on-surface synthesis under UHV enables formation of zigzag GNRs and in situ visualization of their chemical structures by atomic-resolution scanning probe microscopy. While the on-surface synthesis under UHV is typically costly and has limited scalability, the industrially viable CVD method can allow lower-cost production of large GNR films. We compare the three methods in terms of the affordable GNR structures and the resulting control of their electronic and optical properties together with post-processing for device integration. Further, we provide our views on future perspectives in the field of bottom-up GNRs.

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