Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 610
Filtrar
1.
Acta Pharmacol Sin ; 2021 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-33931765

RESUMO

LianhuaQingwen capsule, prepared from an herbal combination, is officially recommended as treatment for COVID-19 in China. Of the serial pharmacokinetic investigations we designed to facilitate identifying LianhuaQingwen compounds that are likely to be therapeutically important, the current investigation focused on the component Glycyrrhiza uralensis roots (Gancao). Besides its function in COVID-19 treatment, Gancao is able to induce pseudoaldosteronism by inhibiting renal 11ß-HSD2. Systemic and colon-luminal exposure to Gancao compounds were characterized in volunteers receiving LianhuaQingwen and by in vitro metabolism studies. Access of Gancao compounds to 11ß-HSD2 was characterized using human/rat, in vitro transport, and plasma protein binding studies, while 11ß-HSD2 inhibition was assessed using human kidney microsomes. LianhuaQingwen contained a total of 41 Gancao constituents (0.01-8.56 µmol/day). Although glycyrrhizin (1), licorice saponin G2 (2), and liquiritin/liquiritin apioside (21/22) were the major Gancao constituents in LianhuaQingwen, their poor intestinal absorption and access to colonic microbiota resulted in significant levels of their respective deglycosylated metabolites glycyrrhetic acid (8), 24-hydroxyglycyrrhetic acid (M2D; a new Gancao metabolite), and liquiritigenin (27) in human plasma and feces after dosing. These circulating metabolites were glucuronized/sulfated in the liver and then excreted into bile. Hepatic oxidation of 8 also yielded M2D. Circulating 8 and M2D, having good membrane permeability, could access (via passive tubular reabsorption) and inhibit renal 11ß-HSD2. Collectively, 1 and 2 were metabolically activated to the pseudoaldosterogenic compounds 8 and M2D. This investigation, together with such investigations of other components, has implications for precisely defining therapeutic benefit of LianhuaQingwen and conditions for its safe use.

2.
Zhen Ci Yan Jiu ; 46(4): 266-71, 2021 Apr 25.
Artigo em Chinês | MEDLINE | ID: mdl-33931989

RESUMO

OBJECTIVE: To observe the effect of electroacupuncture (EA) at "Zusanli" (ST36) and "Feishu" (BL13) on the expression of autophagy related proteins in the lung tissue of rats with chronic obstructive pulmonary disease (COPD), so as to explore the mechanism of EA underlying improvement of COPD. METHODS: Thirty male SD rats were randomly divided into normal, model and EA groups (n=10 in each group). The COPD model was established by intratracheal infusion of Lipopolysaccharide (LPS, 1 mg/kg) and exposure in cigarette smoke. EA was applied to bilateral ST36 and BL13 for 30 min, once every other day for 2 weeks. The pulmonary function (forced vital capacity ï¼»FVCï¼½, forced expiratory volume in 0.1 s and 0.3 s ï¼»FEV0.1, FEV0.3ï¼½, FEV0.1/FVC and FEV0.3/FVC) was detected by animal pulmonary function analysis system. Histopathological changes of the airway and lung were displayed by H.E. staining. Autophagosomes in the airway and lung tissues were observed by electron microscope. The expression of AMP activated protein kinase (AMPK), mammalian target of rapamycin (mTOR), Unc-51 like autophagy activating kinase 1(ULK1), autophagy related protein ATG6(Beclin1)mRNAs in lung tissue were examined by quantitative real-time PCR. The expression of AMPK, mTOR, ULK1, Beclin1 and microtubule-associated protein 1 light chain 3 (LC3)proteins in lung tissue were examined by Western blot. The contents of tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) in the broncho alveolar lavage fluid (BALF) were assayed by ELISA. RESULTS: Following modeling, the FVC, FEV0.1, FEV0.3, FEV0.1/FVC and FEV0.3/FVC levels were significantly decreased (P<0.01), the infiltration of inflammatory cells and the increase of autophagosomes were obvious in airway and lung tissue, the mRNA and protein expression of AMPK, ULK1, Beclin1 and the ratio of LC3Ⅱ/LC3Ⅰ were increased (P<0.01), while the mRNA and protein expression of mTOR were decreased (P<0.01), the contents of TNF-α and IL-6 in the BALF were increased in the model group compared with the normal group (P<0.01). After EA intervention, all the indexes mentioned above were completely reversed in the EA group relevant to the model group (P<0.01, P<0.05). CONCLUSION: EA at ST36 and BL13 can improve the lung function of COPD rats, which may be related to its effects in inhibiting the autophagy level and reducing the inflammation response in the lung.


Assuntos
Eletroacupuntura , Doença Pulmonar Obstrutiva Crônica , Animais , Proteínas Relacionadas à Autofagia , Pulmão , Masculino , Doença Pulmonar Obstrutiva Crônica/genética , Doença Pulmonar Obstrutiva Crônica/terapia , Ratos , Ratos Sprague-Dawley
3.
Soft Matter ; 2021 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-33972975

RESUMO

Elucidating emergent regularities in intriguing crowd dynamics is a fundamental scientific problem arising in multiple fields. In this work, based on the social force model, we simulate the typical scenario of collective escape towards a single exit and reveal the striking analogy of crowd dynamics and crystallisation. With the outflow of the pedestrians, crystalline order emerges in the compact crowd. In this process, the local misalignment and global rearrangement of pedestrians are well rationalized in terms of the characteristic motions of topological defects in the crystal. Exploiting the notions from the physics of crystallisation further reveals the emergence of multiple fast tracks in the collective escape.

4.
Water Res ; 197: 117095, 2021 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-33862392

RESUMO

A 300m3/d demonstration project of soybean-process wastewater has been established recently with a Spiral Symmetric Stream Anaerobic Bioreactor (SSSAB) as the core. In order to obtain the optimal operation strategy for a full-scale SSSAB and to make it run efficiently and stably in a demonstration project, a Pilot-scale SSSAB (P-SSSAB, effective volume 100 L) was performed for the treatment of soybean-process wastewater over 216 days. The volumetric load rate (VLR) range of the P-SSSAB was 0.32~27.17 kg COD/(m3·d), where the highest VLR [27.17 kg COD/(m3·d)] was 2.01 times to the highest value [13.5 kg COD/(m3·d)] reported. The pH and VFA/ALK of the effluent from the P-SSSAB were in the range of 6.9 up to 9.2 and 0.03 up to 0.17, respectively. The methane yield of the P-SSSAB increased from 0.03 m3/kg COD to 0.47 m3/kg COD, which was 3.36 times to the maximum value (0.14 m3/kg COD) reported. To meet the influent requirement of the aerobic biological treatment in demonstration project (influent COD ≤ 1.5 g/L), the maximum VLR of SSSAB was optimal at about 22 kg COD/(m3·d). By analyzing the sludge bed characteristics of the P-SSSAB, it was obvious that zone I (the bottom of the bed) was the major contributor of the COD removal, while zone III (the upper part of the bed) was the major contributor for the NH4+-N increase. The anaerobic granular sludge (AGS) in the bed showed a good granulation. The average MLVSS/MLSS value in sludge bed was about 0.7, and PN/PS in TB-EPS (zone I, II and III) increased to 6.830, 4.257, and 3.747, respectively. SMA and coenzyme F420 values of zone III were the maximum [666.35 ml CH4/(g VSS·d) and 0.690 mol/g VSS, respectively]. According to the analysis obtained from the 16S rRNA high-throughput sequencing, the microbial community in the AGS had been more specific to the soybean-process wastewater since the bacteria Firmicutes were increased. The relative abundance of microbe which perform direct interspecies electron transfer (DIET) for the syntrophic degradation of VFAs and production of the methane has been increased significantly, such as the bacteria Syntrophomonas and archaea Methanosaeta.


Assuntos
Esgotos , Águas Residuárias , Anaerobiose , Reatores Biológicos , Metano , RNA Ribossômico 16S , Rios , Soja , Eliminação de Resíduos Líquidos
5.
PLoS One ; 16(4): e0250126, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33857222

RESUMO

Amino acid metabolism plays an important role in controlling blood pressure by regulating the production of NO and ROS. The present study examined amino acid levels in the serum of Dahl SS rats and SS.13BN rats fed a low or high salt diet. We observed that 8 of 27 amino acids responded to a high salt diet in SS rats. Thus, we hypothesized that a defect in amino acids may contribute to the development of salt-induced hypertension. L-phenylalanine was used to treat SS rats with a low or high salt diet. The results demonstrated that L-phenylalanine supplementation significantly enhanced the serum nitrite levels and attenuated the high salt-induced hypertension in SS rats. Low levels of BH4 and nitrite and the impaired vascular response to acetylcholine were rescued by L-phenylalanine supplementation. Moreover, increased GTP cyclohydrolase (GCH1) mRNA, levels of BH4 and nitrite, and reduced superoxide production were observed in the kidneys of hypertensive SS rats with L-phenylalanine. The antihypertensive effects of L-phenylalanine might be mediated by enhancing BH4 biosynthesis and decreasing superoxide production from NO synthase, thereby protecting vascular and kidney function with reduced ROS and elevated NO levels. The present study demonstrated that L-phenylalanine supplementation restored vascular function, suggesting L-phenylalanine represented a potential target to attenuate high salt-sensitive hypertension through GCH1-BH4.

6.
Stem Cell Res Ther ; 12(1): 243, 2021 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-33858504

RESUMO

BACKGROUND: The regeneration response of the skin to mechanical stretching in vivo has been explored in reconstructive surgery to repair large-scale deformities. The ability of the skin to regenerate limits the reconstructive outcome. Here, we propose an approach in which autologous stromal vascular fraction (SVF) cells and mechanical stretching are combined to overcome this limitation and promote skin regeneration. METHODS: This randomized, blinded, placebo-controlled clinical trial screened 22 participants undergoing tissue expansion with exhausted regeneration. Twenty eligible participants received intradermal injections of the SVF or placebo treatments. Follow-ups were conducted at 4, 8, and 12 weeks to assess efficacy and at 2 years to assess safety. The primary endpoint was the expanded skin thickness at 12 weeks. The secondary endpoints included skin thickness at 4 and 8 weeks, the expansion index (EI), and the skin texture score at 12 weeks. RESULTS: The skin thickness of the SVF group was significantly higher than that of the control group at both 8 weeks (mean difference 0.78 [95% CI - 1.43 to - 0.11]; p = 0.018) and 12 weeks (0.65 [95% CI - 1.30 to - 0.01]; p = 0.046). In the SVF group, the increase in skin thickness was significant at 4 weeks (0.49 [95% CI - 0.80 to - 0.06]; p = 0.010) to 8 weeks (0.45 [95% CI - 0.92 to 0.02]; p = 0.026) and maintained after 12 weeks, whereas that in the control group was reduced after 8 weeks (0.42 [95% CI - 0.07 to 0.91]; p = 0.037). The SVF group showed greater EI increases than the control group (0.50 [95% CI - 0.00 to 0.99]; p = 0.047). The skin texture scores in the SVF group were greater than those in the control group at 12 weeks. Histologically, SVF-treated expanded skin showed more proliferating cells and blood vessels, and the extracellular matrix volume increased. No severe adverse events occurred. CONCLUSIONS: Transplantation of SVF cells can expedite the potency of mechanical stretch-induced skin regeneration and provide clinical reconstruction with plentiful tissue. TRIAL REGISTRATION: This trial was registered with the Chinese Clinical Trial Registry, ChiCTR2000039317 (registered 23 October 2020-retrospectively registered).

8.
Food Chem ; 357: 129522, 2021 Apr 08.
Artigo em Inglês | MEDLINE | ID: mdl-33872871

RESUMO

The influences of the different structural flaxseed lignans on flaxseed oil (FO) emulsions during storage and digestion were investigated, focusing on their interfacial behavior. From perspective of interface, more than 60% of secoisolariciresinol (SECO) and the acidic hydrolysates of flaxseed lignan macromolecule (FLEH) were located on the interface of FO emulsions. It improved the stability of FO emulsions both during storage and digestion by inhibiting of free radical penetration and improving their targeted antioxidative activity. By comparison, the secoisolariciresinol diglucoside (SDG) and the alkaline hydrolysates of flaxseed lignan macromolecule (FLE) largely located in the aqueous and exerted lower antioxidative efficiency in emulsions. Moreover, SDG, SECO, FLE and FLEH slowed down the digestive rate of FO in emulsions, which might be due to flaxseed lignans inhibited the activity of digestive enzymes. These findings suggested that the different structural flaxseed lignans had the potential as antioxidants in emulsions during storage and digestion.

9.
Photodiagnosis Photodyn Ther ; : 102308, 2021 Apr 23.
Artigo em Inglês | MEDLINE | ID: mdl-33901691

RESUMO

In recent years, glioma has become one of the main diseases threatening human health, with a low cure rate and a high mortality rate. Therefore, correct diagnosis and treatment are essential for patients. This research aims to use mid-infrared spectroscopy combined with machine learning algorithms to identify patients with glioma. The glioma infrared spectra and the control group serum are smoothed and normalized, then the principal component analysis (PCA) algorithm is used to reduce the data dimensionality, and finally, the particle swarm optimization-support vector machine (PSO-SVM), backpropagation (BP) neural network and decision tree (DT) model are established. The classification accuracy of the three models was 92.00%, 91.83%, 87.20%, and the AUC values were 0.919, 0.945, and 0.866, respectively. The results show that PCA-PSO-SVM has a better classification effect. This study shows that mid-infrared spectroscopy combined with machine learning algorithms has great potential in the application of non-invasive, rapid and accurate identification of glioma patients.

10.
Artigo em Inglês | MEDLINE | ID: mdl-33905916

RESUMO

PURPOSE: Insufficient physical activity is a public health concern in Indonesia, but there are no valid Indonesian tools for exploring the influences. Due to cultural and contextual differences, the Indonesian version of the Influence on Physical Activity Instrument (IPAI-I) was developed and tested the psychometric properties in community obese-adults. METHODS: The cross-sectional study enrolled 846 obese-adults in Indonesian community health-centre. The IPAI was translated with culturally appropriate Indonesian. Using Exploratory Factor Analysis (EFA) and Confirmatory Factor Analysis (CFA) assessed the psychometric properties. The scale reliability was tested by Cronbach's α for internal consistency and intra-class correlation coefficient (ICC) for stability. RESULTS: Through EFA via Principal Axis Factoring with Oblimin rotation, the 21-item IPAI-I exhibited cross-cultural appropriateness, readability with three factors in the individual domain, one factor in the support domain, and two factors in the environment domain. The indices of good fit model for three domains testing by a second-order CFA was achieved at χ2/df=1.5, goodness of fit index = .93, root mean square error of approximation = .039 (p < .001). Furthermore, a high correlation (r = .52; p < .001) was between the support domain and the environment domain, but the two domains had a very low correlation with the individual domain, respectively. The test-retest reliability was by ICC with .73 - .92 and Cronbach's α with .82 - .91 among six-factor, respectively. CONCLUSIONS: The IPAI-I is a valid, reliable instrument to assess in Indonesian context. Enhancing ecological effects are high-priority to increase physical activity for community obese-individual.

11.
Biosens Bioelectron ; 185: 113265, 2021 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-33930755

RESUMO

As a severe stage of cancers, peritoneal carcinomatosis should be frequently monitored by means of ascites analysis. Nevertheless, the analysis process is traumatic and time-consuming in clinical practice. In this study, an implantable platinum nanotree microelectrode with a wireless, battery-free and flexible electrochemical patch was developed for in vivo and real-time peritoneal glucose detection to monitor peritoneal carcinomatosis. As the core of implantable microelectrode, platinum trees were synthesized by one-step electrodeposition method and highly sensitive to glucose detection. The platinum nanotree microelectrode was implantable in peritoneal cavity in minimally invasive way. A flexible circuit patch could execute electrochemical test and realize wireless power harvesting and data interaction with a near field communication (NFC)-enabled smartphone. The whole system could detect glucose dynamics in vivo in rat peritoneal cavity. Furthermore, the accuracy of this system was validated in ascites of patients. In this way, the system could offer hassle-free, rapid and minimally invasive opportunities toward peritoneal carcinomatosis monitoring.

12.
Nanomaterials (Basel) ; 11(4)2021 Mar 27.
Artigo em Inglês | MEDLINE | ID: mdl-33801680

RESUMO

The trefoil and cinquefoil vector field are of essential significance for fundamental topology properties as the Hopf link and trefoil knots in the light field. The spatially multiplexing metasurfaces were designed with two sets of periodical nanoslits arranged alternately, each had independent geometric spiral phases and metalens phases to produce and focus vortex of the corresponding circular polarized (CP) light. By arranging the orientations of the two slit sets, the two CP vortices of the desired topological charges were obtained, the superposition of the vortices were realized to generate the vector field. With the topological charges of the vortices set to one and two, and three and two, respectively, the focused trefoil and cinquefoil vector light fields were acquired. The work would be important in broadening the applications of metasurface in areas as vector beam generations and topology of light field.

13.
Pharmaceuticals (Basel) ; 14(3)2021 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-33803943

RESUMO

Acute heart failure (AHF) commonly arises from decompensated chronic heart failure or sudden structural and functional breakdown causing a decrease in cardiac contractility and consequently fluid accumulation and systemic congestion. Current treatment for AHF aims at reducing fluid overload and improving hemodynamic which results in quick symptom relief but still poor prognostic outcome. This study utilizes a zebrafish AHF model induced by aristolochic acid (AA) to look for natural products that could attenuate the progression of AHF. The project started off by testing nearly seventy herbal crude extracts. Two of the positive extracts were from Chinese water chestnuts and are further studied in this report. After several rounds of chromatographical chemical fractionation and biological tests, a near pure fraction, named A2-4-2-4, with several hydrophilic compounds was found to attenuate the AA-induced AHF. A2-4-2-4 appeared to inhibit inflammation and cardiac hypertrophy by reducing MAPK signaling activity. Chemical analyses revealed that the major compound in A2-4-2-4 is actually lactate. Pure sodium lactate showed attenuation of the AA-induced AHF and inflammation and cardiac hypertrophy suppression as well, suggesting that the AHF attenuation ability in A2-4-2-4 is attributable to lactate. Our studies identify lactate as a cardiac protectant and a new therapeutic agent for AHF.

14.
Nanomaterials (Basel) ; 11(4)2021 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-33810485

RESUMO

We investigated the optical spin Hall effect (OSHE) of the light field from a closed elliptical metallic curvilinear nanoslit instead of the usual truncated curvilinear nanoslit. By making use of the characteristic bright spots in the light field formed by the noncircular symmetry of the elliptical slit and by introducing a method to separate the incident spin component (ISC) and converted spin component (CSC) of the output field, the OSHE manifested in the spot shifts in the CSC was more clearly observable and easily measurable. The slope of the elliptical slit, which was inverse along the principal axes, provided a geometric phase gradient to yield the opposite shifts of the characteristic spots in centrosymmetry, with a double shift achieved between the spots. Regarding the mechanism of this phenomenon, the flip of the spin angular momentum (SAM) of CSC gave rise to an extrinsic orbital angular momentum corresponding to the shifts of the wavelet profiles of slit elements in the same rotational direction to satisfy the conservation law. The analytical calculation and simulation of finite-difference time domain were performed for both the slit element and the whole slit ellipse, and the evolutions of the spot shifts as well as the underlying OSHE with the parameters of the ellipse were achieved. Experimental demonstrations were conducted and had consistent results. This study could be of great significance for subjects related to the applications of the OSHE.

18.
Phys Rev Lett ; 126(8): 080602, 2021 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-33709721

RESUMO

The transition between ergodic and many-body localization (MBL) phases lies at the heart of understanding quantum thermalization of many-body systems. Here, we predict a many-body critical (MBC) phase with finite-size scaling analysis in the one-dimensional extended Aubry-André-Harper-Hubbard model, which is different from both the ergodic phase and MBL phase, implying that the quantum system hosts three different fundamental phases in the thermodynamic limit. The level statistics in the MBC phase are well characterized by the so-called critical statistics, and the wave functions exhibit deep multifractal behavior only in the critical region. We further study the half-chain entanglement entropy and thermalization properties and show that the former, in the MBC phase, manifest a volume law scaling, while the many-body states violate the eigenstate thermalization hypothesis. The results are confirmed by the state-of-the-art numerical calculations with system size up to L=22. This work unveils a novel many-body phase which is extended but nonthermal.

19.
Sci Rep ; 11(1): 6229, 2021 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-33737604

RESUMO

It remained inconclusive whether patients with peptic ulcer disease had a higher risk of head and neck cancer (HNC). Therefore, we enrolled 109,360 patients with peptic ulcer disease and matched for age and sex with 218,720 controls from the Taiwan National Health Insurance Research Database between January 1, 1997 and December 31, 2013.The HNC incidence rate was 1.33-fold higher in the peptic ulcer group than in the control group (7.52 vs. 5.68 per 100,00 person-years; crude relative risk: 1.33; 95% confidence interval [CI]: 1.08-1.63) after > 6 years of follow-up. However, in the peptic ulcer subgroup with H. pylori treatment, HNC risk was not significantly different from that of the control group (crude relative risk: 1.12; 95% CI: 0.86-1.46). Moreover, the population with peptic ulcers had the highest risk of laryngeal and hypopharyngeal cancer (adjusted HR: 2.27 [95% CI: 1.16-4.44] and 2.00 [95% CI, 1.13-3.55]), respectively. This observational study suggested that peptic ulcer disease is associated with an increased incidence of laryngeal and hypopharyngeal cancer and H. pylori treatment may have a role in preventing HNC in patients with peptic ulcer disease.

20.
Adv Ther (Weinh) ; : 2000224, 2021 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-33786369

RESUMO

SARS-CoV-2 caused the emerging epidemic of coronavirus disease in 2019 (COVID-19). To date, there are more than 82.9 million confirmed cases worldwide, there is no clinically effective drug against SARS-CoV-2 infection. The conserved properties of the membrane fusion domain of the spike (S) protein across SARS-CoV-2 make it a promising target to develop pan-CoV therapeutics. Herein, two clinically approved drugs, Itraconazole (ITZ) and Estradiol benzoate (EB), are found to inhibit viral entry by targeting the six-helix (6-HB) fusion core of SARS-CoV-2 S protein. Further studies shed light on the mechanism that ITZ and EB can interact with the heptad repeat 1 (HR1) region of the spike protein, to present anti-SARS-CoV-2 infections in vitro, indicating they are novel potential therapeutic remedies for COVID-19 treatment. Furthermore, ITZ shows broad-spectrum activity targeting 6-HB in the S2 subunit of SARS-CoV and MERS-CoV S protein, inspiring that ITZ have the potential for development as a pan-coronavirus fusion inhibitor.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...