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1.
Phytochem Anal ; 2021 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-33949013

RESUMO

INTRODUCTION: With the wide application of Scutellaria barbata D. Don for hepatitis and mastitis, its quality control issues have also received increasing attention. Based on the multi-component and multi-target characteristics of traditional Chinese medicine, there is an urgent need to establish a quality evaluation system. OBJECTIVES: This study intends to integrate the "quality-activity-quantification" strategy and establish an activity-related quality control method to ensure the safety and effectiveness of S. barbata. MATERIAL AND METHODS: Ultra-high performance liquid chromatography/ion mobility-quadrupole time-of-flight mass spectrometry (UPLC/IM-QTOF-MS) was used to characterize the chemical components of S. barbata, and network pharmacological analysis was carried out on the identified components. The index components were determined on the basis of comprehensive activity prediction results and content information. At the same time, the contents of 16 batches of S. barbata from different origins were determined. RESULTS: A total of 94 compounds were identified according to mass spectrometric data, 12 of which were isolated and structure-confirmed by nuclear magnetic resonance technology. Network pharmacological analysis was applied to predict their key targets and the major pathways mediating their anti-inflammatory effects. On the basis of comprehensive activity prediction and content information, five components were chosen as crucial quality indicators of S. barbata, including scutellarin, scutellarein, luteolin, apigenin, and hispidulin. CONCLUSION: In this study, 16 different S. barbata batches were compared, and five quality indicators were determined on the basis of qualitative and activity results. The present study provides useful information for evaluating the quality of S. barbata in different areas, and also provides a new basis for the development of quality evaluation methods.

2.
J Infect Dev Ctries ; 15(4): 490-500, 2021 04 30.
Artigo em Inglês | MEDLINE | ID: mdl-33956648

RESUMO

INTRODUCTION: Nutritional status has been defined as an individual's health condition. The relationship between the progression of COVID-19 and Nutritional status is still unclear. We analyzed the clinical characteristics of 342 coronavirus disease 2019 (COVID-19) patients, and analyzed the relationship between the progression of COVID-19 and Nutritional status. METHODOLOGY: 342 COVID-19 were enrolled from ten different hospitals in China. The clinical characteristics were collected and analyzed. RESULTS: The body mass index (BMI) of the mild patients (Group A) was higher than those in severe patients (Group B) and critical patients (Group C); The lactate dehydrogenase (LDH) level of Group A was lower than those of the other two groups; Sex, age, and BMI, was strongly correlated with Clinical classification (CT); Among the laboratory test results, Neutrophil (NEU%), Aspartate aminotransferase (AST), LDH, and blood glucose (BG) were positively correlated with CT; Lymphocyte ( LYM%), Platelet (PLT), Albumin (ALB), and Creatinine (Cr) were negatively correlated with CT. BMI, NEU%, LYM%, ALB, Cr, and PLT are all protective factors that affect CT. CONCLUSION: People with poor nutritional status (lower BMI and ALB) have a higher risk of developing severe disease after infection with SARS-CoV-2. In the clinical treatment of COVID-19, individualized nutritional support is very important for the rehabilitation of patients.

3.
Medicine (Baltimore) ; 100(10): e25047, 2021 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-33725889

RESUMO

RATIONALE: Nevus sebaceous (NS) is a lesion caused by congenital hyperplastic disorder of the sebaceous glands. It commonly noted in the scalp and face and rarely in the trunk, neck, or oral mucosa. We present a rare case of a lesion arising in the genital region. PATIENT CONCERNS: A 47-year-old woman complained of a gradual increase in the size of her bilateral labia minora over 2 years, which affected her sexual life and caused walking difficulty. She was admitted to the Department of Obstetrics and Gynecology. On physical examination, no ulcer, discharge, and vulval or vaginal bleeding were found. The bilateral inguinal lymph nodes were not palpable, bilateral labia minora were asymmetric, and the right side was evidently bigger than the left. The labia minora had serrated edges and numerous papillae with a maximum diameter of 0.5 cm. The vagina, cervix, and uterus with its attachments were normal. Blood samples tested negative for human immunodeficiency virus, human papilloma virus, hepatitis B virus, and hepatitis C virus. DIAGNOSIS: A diagnosis of NS of the bilateral labia minora was made following histopathological examination of the resected specimen. INTERVENTION: The bilateral labia minora lesions were resected general anesthesia on August 29, 2016. The operation was successful, and intraoperative blood loss was about 10 ml. OUTCOMES: After 40 months of postoperative follow-up, no recurrence or appearance of other tumors were noted. LESSONS: We recommend surgical removal of lesions in the genital area during adolescence or before adulthood. Adolescence may be the best period for surgical intervention owing to a greater risk of malignant change in adulthood. On the other hand, surgical risk should be avoided in children considering the low incidence of malignant transformation.


Assuntos
Nevo/diagnóstico , Neoplasias das Glândulas Sebáceas/diagnóstico , Neoplasias Vulvares/diagnóstico , Diagnóstico Diferencial , Feminino , Procedimentos Cirúrgicos em Ginecologia , Humanos , Pessoa de Meia-Idade , Nevo/patologia , Nevo/cirurgia , Neoplasias das Glândulas Sebáceas/patologia , Neoplasias das Glândulas Sebáceas/cirurgia , Glândulas Sebáceas/patologia , Glândulas Sebáceas/cirurgia , Sífilis/diagnóstico , Vulva/patologia , Neoplasias Vulvares/patologia , Neoplasias Vulvares/cirurgia
4.
Biosci Rep ; 41(3)2021 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-33624794

RESUMO

Light plays a direct crucial role in the switch between sleep and arousal and the regulation of physiology and behaviour, such as circadian rhythms and emotional change. Artificial lights, which are different from natural light sources with a continuous light spectrum, are composed of three single-colour lights and are increasingly applied in modern society. However, in vivo research on the mechanisms of blue light-regulated sleep and arousal is still insufficient. In this work, we detected the effects of inserting white or blue light for 1 h during the dark period on the wheel-running activity and sucrose preference of C57 mice. The results showed that blue light could induce delays in sleep and arousal-promoting responses. Furthermore, this lighting pattern, including blue light alone, induced depressive-like emotions. The c-fos expression in the blue light group was significantly higher in the arcuate hypothalamic nucleus (Arc) and significantly lower in the cingulate cortex (Cg) and anterior part of the paraventricular thalamic nucleus (PVA) than in the white light group. Compared with the white light group, the phospho-ERK expression in the paraventricular hypothalamic nucleus (PVN) and PVA was lower in the blue light group. These molecular changes indicated that certain brain regions are involved in blue light-induced response processes. This study may provide useful information to explore the specific mechanism of special light-regulated physiological function.

5.
Genome ; 2021 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-33617379

RESUMO

Molecular identification of acaroid mites is difficult because of the scarcity of molecular data in GenBank. Here, acaroid mites collected from ground flour dust in Xi'an China were preliminarily morphologically classified/grouped. Universal primers were then designed to amplify and screen suitable DNA barcodes for identifying these mites. Sixty mite samples were morphologically classified into six groups. Groups 1-2 were identified to Dermatophagoides farinae, and Tyrophagus putrescentiae; while Groups 3-6 were not identified to the species level. ITS2 exhibited higher efficiency in molecular identification in comparison with COI, 12S, and 16S. Groups 1-6 were identified as D. farinae, T. putrescentiae, Suidasia nesbitti, Chortoglyphus arcuatus, Lepidoglyphus destructor, and Gohieria sp., respectively. The phylogenetic results were consistent with the morphological classification. Group 6 was further identified as G. fusca according to the morphology of reproductive foramen. We conclude that the use of ITS2 and the availability of universal primers provides an ideal DNA barcode for molecular identification of acaroid mites. The use of multiple target genetic markers in conjunction with morphological approaches will improve the accuracy of Acaridida identification. Key words: acaroid mites, Taxonomy, universal primers, molecular identification, DNA barcode.

6.
J Asthma ; : 1-10, 2021 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-33629923

RESUMO

OBJECTIVE: To identify the factors of family management affecting asthma control status in school-age children with asthma in China. METHOD: The cross-sectional descriptive study was conducted among 139 children with asthma and their parents. The age range of the children was 7 to 14 years of age (Mage = 9.85; 76.26% boys). Eight dimensions (Children Identity, View of Condition, Management Mindset, Parental Mutuality, Parenting Philosophy, Management Approach, Family Focus, Future Expectation) of the Family Management Scale for Children with Asthma (FMSCA) were used as factors of family management. The Asthma Control Test (ACT) and the Children Asthma Control Test (C-ACT) were used to measure the asthma control status of children. A parental questionnaire was used to collect information regarding demographic data of familial socioeconomic status, general data about the child, and medical services status (Follow-Up Plan, received manual of asthma education, attended a lecture on asthma) received from medical institutions. A multivariate ordinal logistic regression model was performed. RESULTS: Factors significantly associated with asthma control were "Follow-Up Plan" (OR, 2.004; 95% CI, 1.009-3.981), "Attended a Lecture on asthma" (OR, 2.586; 95% CI, 1.103-6.066) and two dimensions of the FMSCA, "Children Identity" (OR = 1.133; 95% CI, 1.024-1.254) and "Family Focus" (OR = 1.114; 95% CI, 1.007-1.232). CONCLUSION: This study shows that asthma control status of school-age children in China is related to the parents' views of their child as having a "normal condition" and the parents' satisfaction with the balance between asthma related management and other aspects of family life.

7.
Org Lett ; 2021 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-33635659

RESUMO

Reported herein is the first example of the synthesis of carbazoles via oxidative cyclization of 3-alkenylindoles with styrenes under visible light. The irradiation of a catalytic amount of [Ir(dtbbpy)(ppy)2][PF6] as the photocatalyst enables various 3-alkenylindoles and styrenes to undergo tandem [2 + 2] cycloaddition/rearrangement, thereby leading to carbazole derivatives in good to excellent yields under aerobic conditions. Mechanistic studies reveal that photoinduced energy transfer followed by electron transfer is responsible for the tandem reaction.

8.
IEEE J Biomed Health Inform ; 25(5): 1373-1384, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33434140

RESUMO

Remote photoplethysmography (rPPG) is a non-contact technique for measuring cardiac signals from facial videos. High-quality rPPG pulse signals are urgently demanded in many fields, such as health monitoring and emotion recognition. However, most of the existing rPPG methods can only be used to get average heart rate (HR) values due to the limitation of inaccurate pulse signals. In this paper, a new framework based on generative adversarial network, called PulseGAN, is introduced to generate realistic rPPG pulse signals through denoising the chrominance (CHROM) signals. Considering that the cardiac signal is quasi-periodic and has apparent time-frequency characteristics, the error losses defined in time and spectrum domains are both employed with the adversarial loss to enforce the model generating accurate pulse waveforms as its reference. The proposed framework is tested on three public databases. The results show that the PulseGAN framework can effectively improve the waveform quality, thereby enhancing the accuracy of HR, the interbeat interval (IBI) and the related heart rate variability (HRV) features. The proposed method significantly improves the quality of waveforms compared to the input CHROM signals, with the mean absolute error of AVNN (the average of all normal-to-normal intervals) reduced by 41.19%, 40.45%, 41.63%, and the mean absolute error of SDNN (the standard deviation of all NN intervals) reduced by 37.53%, 44.29%, 58.41%, in the cross-database test on the UBFC-RPPG, PURE, and MAHNOB-HCI databases, respectively. This framework can be easily integrated with other existing rPPG methods to further improve the quality of waveforms, thereby obtaining more reliable IBI features and extending the application scope of rPPG techniques.

9.
Comput Biol Med ; 130: 104188, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33421824

RESUMO

Myoelectric interfaces have received much attention in the field of prosthesis control, neuro-rehabilitation systems and human-computer interaction. However, when different users perform the same gesture, the electromyography (EMG) signals can vary greatly. It is essential to design a multiuser myoelectric interface that can be simply used by novel users while maintaining good gesture classification performance. To cope with this problem, canonical correlation analysis (CCA) has been used to extract the inherent user-independent properties of EMG signals generated from the same gestures from multiple users and demonstrated superior performance. In this paper, we move forward to propose a novel framework based on CCA and optimal transport (OT), termed as CCA-OT. By optimal transport, the discrepancies in data distribution between the transformed feature matrix from the training and the testing sets can be further reduced. Experimental results on the defined 13 Chinese sign language gestures performed by 10 intact-limbed subjects demonstrated that the classification rate of our proposed CCA-OT framework is significantly higher than that of the CCA-only framework with an 8.49% promotion, which shows the necessity to reduce the drift in probability distribution functions (PDFs) of the different domains. The CCA-OT framework provides a promising method for the multiuser myoelectric interface which can be easily adapted to new users. This improvement will further facilitate the widespread implementation of myoelectric control systems using pattern recognition techniques.

10.
Cell Res ; 2021 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-33469157

RESUMO

Ca2+ channels are essential to cell birth, life, and death. They can be externally activated by optogenetic tools, but this requires robust introduction of exogenous optogenetic genes for expression of photosensitive proteins in biological systems. Here we present femtoSOC, a method for direct control of Ca2+ channels solely by ultrafast laser without the need for optogenetic tools or any other exogenous reagents. Specifically, by focusing and scanning wavelength-tuned low-power femtosecond laser pulses on the plasma membrane for multiphoton excitation, we directly induced Ca2+ influx in cultured cells. Mechanistic study reveals that photoexcited flavins covalently bind cysteine residues in Orai1 via thioether bonds, which facilitates Orai1 polymerization to form store-operated calcium channels (SOCs) independently of STIM1, a protein generally participating in SOC formation, enabling all-optical activation of Ca2+ influx and downstream signaling pathways. Moreover, we used femtoSOC to demonstrate direct neural activation both in brain slices in vitro and in intact brains of living mice in vivo in a spatiotemporal-specific manner, indicating potential utility of femtoSOC.

11.
J Infect Dev Ctries ; 14(11): 1264-1273, 2020 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-33296339

RESUMO

INTRODUCTION: To date, the relationship between the causative pathogens and the changes of hematological parameters was rarely referred and deserves further investigation. METHODOLOGY: A total of 825 adult patients, including 134 negative blood cultures patients and 691 bloodstream infection (BSI) patients, were screened for eligibility in this study. Receiver operating characteristic curves and binary logistic regression models were used to assess the power of hematological parameters to distinguish patients with BSI caused by different pathogens. RESULTS: Except for platelet-to-lymphocyte ratio (PLR) and platelet larger cell count (P-LCC), the other hematological parameters investigated in the study were significantly different in patients with BSI caused by different pathogens, including Candida. The specific combinations of lymphocyte count (LYM), platelet count (PLT), neutrophil-to-lymphocyte ratio (NLR), mean platelet volume (MPV), MPV-to-PLT ratio (MPV/PLT), platelet larger cell ratio (P-LCR), and C-reactive protein (CRP) can improve the ability to distinguish various BSI from negative blood cultures. The highest area under the curve of was 0.753 (95% CI 0.709-0.797) for positive blood cultures, 0.715 (95% CI 0.658-0.771) for Gram-positive pathogens BSI, 0.777 (95% CI 0.730-0.824) for Gram-negative pathogens BSI, 0.797 (95% CI 0.747-0.846) for Escherichia coli BSI, 0.943 (95% CI 0.899-0.987) for Enterobacter aerogenes BSI, 0.830 (95% CI 0.740-0.921) for Pseudomonas aeruginosa BSI, and 0.767 (95% CI 0.695-0.839) for Staphylococcus aureus BSI. CONCLUSIONS: The specific combinations of hematological parameters can improve the power to distinguish patients with BSI caused by different pathogens. Attention to these parameters can be easily integrated into daily medical activities, without extra costs.

12.
BMC Pulm Med ; 20(1): 308, 2020 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-33225932

RESUMO

BACKGROUND: The recurrence of positive SARS-CoV-2 nucleic acid test results in patients with COVID-19 is becoming more important and warrants more attention. CASE PRESENTATION: This study reports 2 cases, a child with mild COVID-19 and an adult female with moderate COVID-19, who were discharged after three consecutive negative nucleic acid tests and were later readmitted to the hospital for recurrence of SARS-CoV-2 nucleic acid positivity. By tracking the patients' symptoms, serum antibodies, and imaging manifestations after readmission, we found that they showed a trend of gradual improvement and recovery throughout treatment. They were cured without additional treatment, with the appearance of antibodies and the recovery of immune functions. CONCLUSIONS: It is deemed extremely necessary to improve the discharge standard of care. At the same time, nucleic acid detection is recommended to increase the dynamic monitoring of serum antibodies and imaging, strengthen the management of discharged patients, and appropriately extend the home or centralized isolation time.


Assuntos
Betacoronavirus , Técnicas de Laboratório Clínico , Infecções por Coronavirus/diagnóstico , Pneumonia Viral/diagnóstico , Criança , Infecções por Coronavirus/terapia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Pandemias , Pneumonia Viral/terapia , Recidiva
13.
Front Microbiol ; 11: 572642, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33193173

RESUMO

The bacterial genus Providencia is Gram-negative opportunistic pathogens, which have been isolated from a variety of environments and organisms, ranging from humans to animals. Providencia alcalifaciens, Providencia rettgeri, and Providencia stuartii are the most common clinical isolates, however, these three species differ in their pathogenicity, antibiotic resistance and environmental adaptation. Genomes of 91 isolates of the genus Providencia were investigated to clarify their genetic diversity, focusing on virulence factors, antibiotic resistance genes, and environmental adaptation genes. Our study revealed an open pan-genome for the genus Providencia containing 14,720 gene families. Species of the genus Providencia exhibited different functional constraints, with the core genes, accessory genes, and unique genes. A maximum-likelihood phylogeny reconstructed with concatenated single-copy core genes classified all Providencia isolates into 11 distant groups. Comprehensive and systematic comparative genomic analyses revealed that specific distributions of virulence genes, which were highly homologous to virulence genes of the genus Proteus, contributed to diversity in pathogenicity of Providencia alcalifaciens, Providencia rettgeri, and Providencia stuartii. Furthermore, multidrug resistance (MDR) phenotypes of isolates of Providencia rettgeri and Providencia stuartii were predominantly due to resistance genes from class 1 and 2 integrons. In addition, Providencia rettgeri and Providencia stuartii harbored more genes related to material transport and energy metabolism, which conferred a stronger ability to adapt to diverse environments. Overall, our study provided valuable insights into the genetic diversity and functional features of the genus Providencia, and revealed genetic mechanisms underlying diversity in pathogenicity, antibiotic resistance and environmental adaptation of members of this genus.

14.
J Med Virol ; 2020 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-33174624

RESUMO

We aimed to describe liver injury and identify the risk factors of liver injury in coronavirus disease (COVID-19) patients without chronic liver diseases (CLD). The clinical data of 228 confirmed COVID-19 patients without CLD were retrospectively collected from ten hospitals in Jiangsu, China. Sixty-seven (29.4%) of 228 patients without CLD showed abnormal liver function on admission, including increased alanine aminotransferase (ALT) (25 [11.0%]) U/L, aspartate aminotransferase (AST) 30 [13.2%]) U/L, gamma-glutamyl transferase (GGT) 28 [12.4%]) U/L, total bilirubin (Tbil) 16 [7.0%] µmol/L, and alkaline phosphatase (ALP) 10 [4.5%]) U/L. During hospitalization, 129 (56.3%) of 228 patients showed abnormal liver function, including elevated ALT (84 [36.8%]), AST (58 [25.4%]), GGT (67 [29.5%]), and Tbil (59 [25.9%]). Age over 50 years (odds ratio [OR], 2.086; 95% confidence interval [CI], 1.030-4.225; p = .041), male sex (OR, 2.737; 95% CI, 1.418-5.284; p = .003), and lopinavir-ritonavir (OR, 2.504; 95% CI, 1.187-5.283; p = .016) were associated with higher risk of liver function abnormality, while the atomized inhalation of interferon α-2b (OR, 0.256; 95% CI 0.126-0.520; p < .001) was associated with reduced risk of liver function abnormality during hospitalization. Mild to moderate liver injury was common in COVID-19 patients in Jiangsu, China. Age over 50 years, male sex, and lopinavir-ritonavir were the independent risk factors of liver impairment in COVID-19 patients during hospitalization.

15.
Diagn Microbiol Infect Dis ; 99(2): 115243, 2020 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-33130506

RESUMO

Mycobacterium tuberculosis (MTB) is commonly diagnosed via the GeneXpert MTB/RIF assay. The cycle threshold (Ct) value of probe A from this assay produced a fluorescence signal upon Mycobacterium intracellulare detection. No other nontuberculous mycobacteria (NTM) exhibited positive probe signals. Using a confirmed mycobacterial culture as a standard, probe A of the assay exhibited 84% sensitivity (95% confidence interval [CI]: 71%-97%) and 50% specificity (95% CI: 37%-63%) for clinical samples. For M. intracellulare strains, probe A exhibited 90% sensitivity (95% CI: 80%-100%) and 50% specificity (95% CI: 37%-63%). The identity of the amino acid sequence and 81-bp core region of rpoB from MTB and NTM suggested that the highly conserved property might be associated with a mismatch between the probes and the chromosomal DNA target. Probe A yielded a positive signal upon M. intracellulare detection; thus, probe A may help diagnose M. intracellular infections.

16.
Pediatr Transplant ; : e13825, 2020 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-33131184

RESUMO

BACKGROUND: HSCT is the only proven curative therapy for JMML. Matching donor and recipient HLA alleles is considered optimal to reduce the risk of GVHD after HSCT but is not always possible. Only a limited number of studies have compared the influence of HLA disparities on HSCT outcomes for patients with JMML. METHODS: We conducted a retrospective study among 47 children with JMML who received related or unrelated unmanipulated HSCT (March 2010-October 2018). Among our participants, 27 (57.4%) donor-recipient pairs had 0-1 HLA disparities (Group 1: HLA-matched or ≤1 allele/antigen mismatch donor) and 20 (42.6%) had ≥2 HLA disparities (Group 2: 2-3 mismatched/haploidentical donors). RESULTS: The median follow-up period was 26.0 months (range: 1-105 months), and the 5-year probabilities of DFS and RI for the whole cohort were 54.6 ± 7.7% and 34.8 ± 15.0%, respectively. Compared to Group 1, Group 2 patients had a significantly lower RI (5.3 ± 10.5% vs 55.5 ± 20.9%, P Ë‚ .001), though similar rates of grade II-IV acute GVHD (60.0 ± 22.4% vs 33.3 ± 18.2%, P = .08), grade III-IV acute GVHD (25.0 ± 19.5% vs 7.4 ± 10.1%, P = .08), chronic GVHD (30.0 ± 20.9% vs 34.9 ± 18.8%, P = .85), NRM (20.0 ± 18.0% vs 3.9 ± 7.7%, P = .07), and DFS (74.4 ± 9.9% vs 41.3 ± 10.0%, P = .08). CONCLUSIONS: Disease relapse remains the major cause of treatment failure in JMML patients, especially in patients receiving HLA-matched and limited HLA-mismatched HSCT. Our findings suggest that donor-recipient HLA disparities may improve the outcome of HSCT in children with JMML.

17.
Front Public Health ; 8: 584964, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33194991

RESUMO

Background: There has been a considerable concern about cancer induction in response to radiation exposure. However, only a limited number of studies have focused on the biological effects of below-background radiation (BBR) in deep underground environments. To improve our understanding of the effects of BBR on cancer, we studied its biological impact on well-differentiated laryngeal squamous cell carcinoma cells (FD-LSC-1) in a deep underground laboratory (DUGL). Methods: The growth curve, morphological, and quantitative proteomic experiments were performed on FD-LSC-1 cells cultured in the DUGL and above-ground laboratory (AGL). Results: The proliferation of FD-LSC-1 cells from the DUGL group was delayed compared to that of cells from the AGL group. Transmission electron microscopy scans of the cells from the DUGL group indicated the presence of hypertrophic endoplasmic reticulum (ER) and a higher number of ER. At a cutoff of absolute fold change ≥ 1.2 and p < 0.05, 807 differentially abundant proteins (DAPs; 536 upregulated proteins and 271 downregulated proteins in the cells cultured in the DUGL) were detected. KEGG pathway analysis of these DAPs revealed that seven pathways were enriched. These included ribosome (p < 0.0001), spliceosome (p = 0.0001), oxidative phosphorylation (p = 0.0001), protein export (p = 0.0001), thermogenesis (p = 0.0003), protein processing in the endoplasmic reticulum (p = 0.0108), and non-alcoholic fatty liver disease (p = 0.0421). Conclusion: The BBR environment inhibited the proliferation of FD-LSC-1 cells. Additionally, it induced changes in protein expression associated with the ribosome, gene spliceosome, RNA transport, and energy metabolism among others. The changes in protein expression might form the molecular basis for proliferation inhibition and enhanced survivability of cells adapting to BBR exposure in a deep underground environment. RPL26, RPS27, ZMAT2, PRPF40A, SNRPD2, SLU7, SRSF5, SRSF3, SNRPF, WFS1, STT3B, CANX, ERP29, HSPA5, COX6B1, UQCRH, and ATP6V1G1 were the core proteins associated with the BBR stress response in cells.

18.
New Microbes New Infect ; : 100814, 2020 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-33204428

RESUMO

The coronavirus disease 2019 (COVID-19) is still global epidemic. Several studies of severe COVID-19 patients regard convalescent plasma (CP) transfusion as an effective therapy. However, there are no significant improvements in the randomized clinical trial of CP treatment. Until now, the data of mild COVID-19 patients transfused CP were lack. This study recruited 8 mild COVID-19 patients received at least one dose of CP transfusion. After CP therapy, all patients improved the clinical symptoms. The level of lymphocyte counts tended to increase, meanwhile LDH, CK and AST tended to decrease. However, CRP of 3 patients increased transiently. The median time that SARS-CoV-2 nucleic acid of the patients turned to negative was 2.5d after CP transfusion. The study shows the potential benefits of CP. Meanwhile, CP probably enhances the inflammatory response to SARS-CoV-2 temporarily in the patients with insufficient antiviral immunity. However, the effects of CP are not permanent.

19.
Chem Commun (Camb) ; 56(92): 14435-14438, 2020 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-33146183

RESUMO

Two donor-acceptor motif fulvene-containing boron complexes were synthesized with fulvene diketonate boron difluoride (FDB) as the organic acceptor. Both difluoroboron complexes present aggregation-induced emission (AIE) properties and cell tracing function with excellent biocompatibility. And mechanochromic luminescence has been accomplished by the synthesis, isolation and characterization of BL2.

20.
Comput Biol Med ; 126: 104050, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33096422

RESUMO

Multi-modal medical imaging has emerged as a general trend in clinical diagnosis and treatment planning. In recent years, great efforts have been made to investigate and develop dual-modality scanners, among which PET/CT is the most widespread one in clinical practice. In this paper, we propose a simple yet effective PET/CT data visualization method that can integrate these two modalities into composite data for better observation. The proposed method consists of three main steps. First, a PET data colorization approach is presented based on a dual-threshold scheme, which applies a pair of high and low thresholds to colorize the PET image. Then, to extract functional information from the PET image more adequately, unlike traditional blending fashion that directly uses the CT image as underlay, we merge the CT and the PET images with a Laplacian pyramid (LP)-based image fusion approach to generate the underlay. Finally, the visualization result is obtained by blending the fused image and the colorized PET image. Experiments are conducted on 5 sets of PET/CT scans that contain 200 paired slices in total. The ClearCanvas software and the method using the presented PET colorization approach but with the CT image as underlay are adopted for comparison. Experimental results demonstrate that the proposed method can achieve more promising performance in terms of both visual perception and quantitative assessment. The code of the proposed method has been made available online athttps://github.com/yuliu316316/Visualization.

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