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1.
Talanta ; 207: 120261, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31594567

RESUMO

Significant progress on circulating tumor cells (CTCs) has profound impact for noninvasive tumor profiling including early diagnosis, treatment monitoring, and metastasis recognition. Therefore, CTCs based liquid biopsy technology is taking a rapid growth in the field of precision oncology. The label-free approaches relied on microfluidic chip stand out from a crowd of methods that suffer from time consuming, extensive blood samples, lost target cells and labor-intensive operation. In this paper, a label-free separation microfluidic device was developed using multistage channel, which took full advantage of inertial lift force. Our strategy demonstrated CTCs were efficiently isolated from untreated human blood samples including antibody conjugation and erythrocyte lysis. This device was applied for isolating human brain malignant glioma cells that were spiked in human peripheral blood samples. The experimental condition was optimized and exhibited an average separation efficiency of ≥ 90% across cell morphological analysis, up to 84.96% purity of collected CTCs and the viability of all cells is >95%, which was better than other one-step CTCs separation methods. Furthermore, the CTCs were successfully separated from untreated clinical blood sample of cancer patient on the proposed microfluidic device. The entire experimental procedures are extremely low-cost and easy manipulation. It is believed that the proposed multistage microfluidic chip can become a promising tool for CTCs separation and early diagnosis of cancer.

2.
Waste Manag ; 101: 180-187, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31622863

RESUMO

With the rapid development of photovoltaic industry, the recycling of waste solar photovoltaic (PV) panels is becoming a critical and global challenge. Considering PV panels recycling is significantly effective and worthwhile to save natural resources and reduce the cost of production, how to selectively recycle valuable components of PV panels is the hot and dominant topic. Different from current mechanical crushing, heat treatment and chemical operation processes, novel and environment-friendly recycling approaches by using high voltage pulse discharge in water, called high voltage fragmentation (HVF), was discussed under different discharge conditions. The results showed that discharging across surface and interior of PV panels produced ablation round holes, sputter metal particles and dendritic channels. The average particle size decreased with the ascent of pulse number and voltage amplitude. Considering the energy consumption, the optimal condition of HVF in this paper was 160 kV for 300 pulses with the energy consumption of 192.99 J/g, crushing the PV panels into particles of 4.1 mm in average (13.7% of the initial size). More particle was distributed among the 0.1-2 mm size fractions as the energy increased. Selective fragmented products, such as Cu, Al, Pb, Ag and Sn, are concentrated on the fractions under 1 mm. Finally, hybrid crushing energy consumption model combined with fractal theory was discussed, which presented close relationship between energy and average particle size. Walker's model (n = 2.047 determined by fractal theory) had the best fitting effect.


Assuntos
Resíduo Eletrônico , Metais , Reciclagem
3.
Food Chem ; 303: 125407, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31466032

RESUMO

Theaflavin (TF), which is the key pigment in black tea, is a health-promoting food component with beneficial effects on humans. However, the interactions by which these effects are transferred and exerted into protein-rich foods are unclear. Here, egg ovalbumin (OVA) was selected as a representative dietary protein to ascertain their binding mechanism. Steady-state, time-resolved fluorescence and isothermal titration calorimetric results showed that TF can interact well with OVA with an affinity magnitude of 104. The noncovalent binding was mainly driven by hydrophobic interaction and hydrogen bonds. Structural analysis displayed that the TF binding pocket significantly overlapped with one of the surrounding specific IgE-binding epitopes, thereby causing a subtle structural adjustment on the secondary conformation of OVA. The biological complexation model that was delineated here will help understand how black tea dyes egg white in tea egg products and for the development of protein-rich carriers in functional foods.


Assuntos
Biflavonoides/química , Camellia sinensis/química , Catequina/química , Clara de Ovo/química , Ovalbumina/química , Pigmentos Biológicos/química , Extratos Vegetais/química , Animais , Galinhas , Humanos , Interações Hidrofóbicas e Hidrofílicas , Cinética , Ligação Proteica , Chá/química
4.
Arch Oral Biol ; 109: 104578, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31589997

RESUMO

OBJECTIVE: The aim of this study was to observe the effect of V. parvula on the physiological activity of S. mutans and elucidate the role of V. parvula on dental caries. DESIGN: We constructed dual-species biofilms formed by V. parvula and S. mutans, and measured the pH dynamics, biofilm growth, Extracellular Polysaccharide (EPS) synthesis, and expression of S. mutans EPS synthesis-associated genes affected by V. parvula. RESULTS: pH dynamics were not altered when V. parvula and S. mutans were co-cultured during a 120 -h test period. However, S. mutans cell number and EPS synthesis in dual-species biofilms were found to be significantly higher than in single-species biofilms. Moreover, expression levels of genes encoding glucosyltransferases (gtfs), gtfB and gtfC specifically, were up-regulated when S. mutans was co-cultured with V. parvula. CONCLUSIONS: Our findings indicate that V. parvula is not, as previously thought, protective and associated with caries health. On the contrary, V. parvula might participate in caries development through interactions with S. mutans. This study suggests that V. parvula may have an impact on the pathogenesis of dental caries.

5.
Environ Sci Technol ; 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31747260

RESUMO

Although arsenic at a high concentration imposes strong selective pressures on microbes, various microbes have been found to grow in As-rich environments. So far, little is known about how microbes can sense and move towards arsenate in environments, and the underlying molecular mechanisms have not been revealed. Here we report the chemotaxis response towards arsenate (As(V)) by Shewanella putrefaciens CN-32, a model dissimilatory metal reducing bacterium (DMRB), and elucidate the mechanisms. We find that S. putrefaciens CN-32 exhibits a chemotactic behavior towards As(V) and diverse electron acceptors. The sense to As(V) by S. putrefaciens CN-32 requires the functional arsenate respiratory reductase, but doesn't depend on its metal-reducing-like respiratory pathway. We observe that such a sense is governed by an energy taxis mechanism and mediated by several methyl-accepting chemotaxis proteins (MCPs), rather than a specific MCP. Moreover, we reveal that the chemotactic signal transduction pathway is conserved in Shewanella, and histidine kinase and flagella-mediated motility are essential for taxis towards As(V). This work reverses the conventional view about arsenic as a chemotactic inhibitor to microbes by revealing the positive chemotaxis of Shewanella to As(V).

6.
Artigo em Inglês | MEDLINE | ID: mdl-31751197

RESUMO

A novel Gram-stain-positive, rod shaped and anaerobic bacterium, designated as W6T, was isolated from Shengli oilfield in China. Strain W6T was observed to grow from 20 to 45 °C with pH 6.5-9.0 (optimally at 40 °C and pH of 7.5) and without addition of NaCl. The major cellular fatty acids were iso-C15 : 0 (29.1%), C14 : 0 (27.0%) and C16 : 0 (12.2%), and the main polar lipids were lipids (L) and aminolipids (AL). The DNA G+C content is 42.9 mol%. Based on 16S rRNA gene sequence analysis, strain W6T showed highest similarities to Tissierella creatinini DSM 9508T (94.9%) and Soehngenia saccharolytica DSM 12858T (94.1%). The morphological, physiological, biochemical, phylogenetic and phylogenomic analyses demonstrated strain W6T (CCAM 534T=DSM 28124T=CGMCC 1.5291T) represents a novel species in a new genus, for which the name Gudongella oleilytica gen. nov. sp. nov. is proposed. The family Tissierellaceae is proposed as a new family containing the genera Anaerosalibacter, Gudongella, Keratinibaculum, Soehngenia, Sporanaerobacter, Tepidimicrobium, Tissierella, Urmitella and species Clostridium ultunense based on the phylogenetic and phylogenomic analyses.

7.
Anesthesiology ; 2019 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-31714269

RESUMO

WHAT WE ALREADY KNOW ABOUT THIS TOPIC: Coupling of neuronal oscillations between brain regions is correlated with higher level brain activityPermutation cross mutual information can be used to evaluate information integration in the electroencephalogram during anesthesia. WHAT THIS ARTICLE TELLS US THAT IS NEW: Using electrocorticography in subjects anesthetized with propofol, the genuine permutation cross mutual information demonstrated that, with loss of consciousness, there was a loss of efficient global information transmission and increased local functional segregation in the cortical network BACKGROUND:: The neurophysiologic mechanisms of propofol-induced loss of consciousness have been studied in detail at the macro (scalp electroencephalogram) and micro (spiking or local field potential) scales. However, the changes in information integration and cortical connectivity during propofol anesthesia at the mesoscopic level (the cortical scale) are less clear. METHODS: The authors analyzed electrocorticogram data recorded from surgical patients during propofol-induced unconsciousness (n = 9). A new information measure, genuine permutation cross mutual information, was used to analyze how electrocorticogram cross-electrode coupling changed with electrode-distances in different brain areas (within the frontal, parietal, and temporal regions, as well as between the temporal and parietal regions). The changes in cortical networks during anesthesia-at nodal and global levels-were investigated using clustering coefficient, path length, and nodal efficiency measures. RESULTS: In all cortical regions, and in both wakeful and unconscious states (early and late), the genuine permutation cross mutual information and the percentage of genuine connections decreased with increasing distance, especially up to about 3 cm. The nodal cortical network metrics (the nodal clustering coefficients and nodal efficiency) decreased from wakefulness to unconscious state in the cortical regions we analyzed. In contrast, the global cortical network metrics slightly increased in the early unconscious state (the time span from loss of consciousness to 200 s after loss of consciousness), as compared with wakefulness (normalized average clustering coefficient: 1.05 ± 0.01 vs. 1.06 ± 0.03, P = 0.037; normalized average path length: 1.02 ± 0.01 vs. 1.04 ± 0.01, P = 0.021). CONCLUSIONS: The genuine permutation cross mutual information reflected propofol-induced coupling changes measured at a cortical scale. Loss of consciousness was associated with a redistribution of the pattern of information integration; losing efficient global information transmission capacity but increasing local functional segregation in the cortical network.

8.
Orthop Surg ; 2019 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-31679187

RESUMO

OBJECTIVE: To determine whether lumbar anatomy parameters are in dynamic change and related factors. METHODS: This is a retrospective study. Participants who did lumbar computed tomography (CT) scanning in Shandong University Qilu Hospital from October 2017 to March 2019 were selected. The 476 participants were randomly selected as male or female, with the age ranging from 17 to 87 years (mean, 55.19; standard deviation, 14.28 years). All the measurements were taken based on the CT scanning image and the measurement of lumbar morphology was conducted using picture archiving and communication systems (PACS). The angle between the horizontal alignment and pedicle center on median sagittal view, the angle between upper endplate and lower endplate on median sagittal view as well as transverse section angle (TSA) using Magerl point in the axial view was determined by reconstructive CT analysis. RESULTS: In the overall participants, the angle between the horizontal alignment and pedicle center on median sagittal view of lumbar one to three was significantly decreased with aging, from 3.90° ± 2.81° to -4.18° ± 6.86° (P = 0.002), 5.60° ± 2.89° to -4.14° ± 5.90° (P = 0.030), and 4.75° ± 2.95° to -2.87° ± 4.68° (P < 0.001), respectively. Additionally, the angle between the horizontal alignment and pedicle center on median sagittal view in male participants of lumbar two was dramatically decreased, from 4.83° ± 2.79° to -4.45° ± 5.97° (P = 0.30). And that of lumbar three in female participants was significantly decreased, from 4.56° ± 2.52° to -2.88° ± 5.03° (P = 0.029). Furthermore, of the overall participants, the angle between upper endplate and lower endplate on median sagittal view of lumbar one to four was associated with aging (P < 0.001, P < 0.001, P = 0.015, P < 0.001, respectively). The angle of lumbar one, two and four in male participants and lumbar one to four in female participants were all significantly related to aging (all P < 0.05). Moreover, in the participants overall, the TSA of lumbar one to three was significantly associated with aging (P = 0.015, P = 0.006 and P = 0.007, respectively). In addition, this angle in lumbar one to lumbar four in male participants were all negatively associated with aging (P = 0.017, P = 0.001, P = 0.005 and P = 0.036, respectively). CONCLUSION: Lumbar anatomy parameters are in dynamic change in an age and gender dependent manner. During spine surgery in elderly patients, more attention should be paid to these anatomic changes.

9.
Artigo em Inglês | MEDLINE | ID: mdl-31729828

RESUMO

BACKGROUND: Dental implants and abutments are exposed to challenging oral environment. Corrosion of these materials can affect the overall performance of titanium implants. PURPOSE: To investigate the effects of biofilm-induced bio-aging on corrosion behavior of different implant materials surface. MATERIALS AND METHODS: Commercial polished titanium (Polish), sand-blasted, large grit, acid-etched surface treated titanium (SLA), microarc oxidation (MAO), and hydroxyapatite (HA) coated titanium were bio-aged with saliva biofilm for 30 days. Titanium surfaces topography, chemical composition, roughness, and water contact angle changes were evaluated. In addition, human gingival fibroblasts (HGFs) adhesion, Streptococcus sanguinis (S. sanguinis) biofilm formation were determined. RESULTS: Surface topography, roughness, and chemical composition have no significant changes for all groups after bio-aging (P > .05). Water contact angle of bio-aged SLA was greatly increased (P < .05). While other groups showed no sign of change (P > .05). Adhesion and proliferation of HGFs on the bio-aged SLA titanium surfaces were decreased (P < .05), but increased on bio-aged Polish and HA titanium (P < .05). S. sanguinis biofilm viability was promoted with bio-aging in HA group (P < .05). CONCLUSIONS: Biological characteristics of Polish, SLA, and HA titanium surfaces were influenced by bio-aging. While MAO group was relatively resistant to saliva biofilm bio-aging.

10.
Int J Biol Macromol ; 2019 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-31706814

RESUMO

In this study, polysaccharides from Stropharia rugosoannulata were isolated and purified by macroporous adsorption resin and ion exchange chromatography to produce SRP-1 and SRP-2. Preliminary structural characteristics of SRP-1 and SRP-2 were also investigated. Our results showed that SRP-1 was neutral, while SRP-2 was acidic polysaccharides. The molar ratios of monosaccharides in SRP-1 and SRP-2 were different, but glucose was the main monosaccharide. Both α-type and ß-type glycosidic linkages were detected in SRP-1, whereas ß-constitution was dominant in SRP-2. Both SRP-1 and SRP-2 had a (1 → 6)-α-D-glucan backbone. Furthermore, the antioxidant activities of polysaccharides against ABTS+, DPPH and OH- radicals were determined in vitro. The results indicated that SRP-2 had a stronger antioxidant capacity than that of SRP-1. Our results may be valuable with regard to the utilization of polysaccharides from S. rugosoannulata as potential pharmaceuticals.

11.
Acta Biomater ; 2019 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-31629895

RESUMO

Infection is a main cause of implant failure. Early implant-related infections often occur in the first 4 weeks post-operation. Inhibiting bacterial adhesion and biofilm formation at the early stage and promoting subsequent implant osseointegration are important for implant success. Our previous studies demonstrated that dimethylaminododecyl methacrylate (DMADDM) provided dental materials with antibacterial effects. In the present study, DMADDM and hydroxyapatite (HA) are loaded on to the titanium (Ti) surface via poly dopamine (PDA) self-polymerization. This local DMADDM-delivery Ti is referred as Ti-PHD. Here we report the two-staged capability of Ti-PHD: (1) in the first stage, releasing DMADDM during the high-infection-risk initial period post-implantation for 4 weeks; (2) then in the second stage, enhancing osteogenesis and promoting osseointegration. Ti-PHD has a porous surface with higher average roughness and greater hydrophilicity than pure Ti. Its biocompatibility is verified in vitro and in vivo. During the first 4 weeks of release, both DMADDM remaining on Ti surface and DMADDM released into the soaking medium greatly reduced the adherence and growth of pathogens. This is further confirmed by the prevention of bone destruction in a rat osteomyelitis model. After releasing DMADDM for 4 weeks, Ti-PHD promotes osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) and new bone formation around the implants in vivo. This article represents the first report on the two-staged, time-dependent antibacterial and osteogenesis effects of Ti-PHD, demonstrating its potential for clinical applications to inhibit implant-associated infections. STATEMENT OF SIGNIFICANCE: The present study develops a two-staged time-dependent system for local dimethylaminododecyl methacrylate (DMADDM) delivery via Ti implant (referred to as Ti-PHD). DMADDM and hydroxyapatite (HA) are loaded on to the Ti surface with poly dopamine (PDA). Ti-PHD can release DMADDM during the high-risk period of infection in the first stage, and then promote osseointegration and new bone formation in the second stage. This bioactive and therapeutic Ti is promising to inhibit infections and enhance implant success.

12.
Artigo em Inglês | MEDLINE | ID: mdl-31566435

RESUMO

The metabolic sensor AMP-activated protein kinase (AMPK) inhibits the epithelial Na+ channel (ENaC), a key regulator of salt reabsorption by the kidney and thus total body volume and blood pressure. Recent studies suggest that AMPK promotes the association of the PAK-interacting exchange factor ß1Pix, 14-3-3 proteins, and the ubiquitin ligase Nedd4-2 into a complex that inhibits ENaC by enhancing Nedd4-2 binding to ENaC and ENaC degradation. Functional ß1Pix is required for ENaC inhibition by AMPK and promotes Nedd4-2 phosphorylation and stability in mouse kidney cortical collecting duct (CCD) cells. Here, we report that AMPK directly phosphorylates ß1Pix in vitro. Among several AMPK phosphorylation sites on ß1Pix detected by mass spectrometry (MS), Ser-71 was validated as functionally significant. Compared to wild-type ß1Pix, overexpression of a phosphorylation-deficient ß1Pix-S71A mutant attenuated ENaC inhibition and the AMPK-activated interaction of both ß1Pix and Nedd4-2 to 14-3-3 proteins in CCD cells. Similarly, overexpression of a ß1Pix-Δ602-611 deletion-tract mutant unable to bind 14-3-3 proteins decreased the interaction between Nedd4-2 and 14-3-3 proteins, suggesting that 14-3-3 binding to ß1Pix is critical for the formation of a ß1Pix/Nedd4-2/14-3-3 complex. With expression of a general peptide inhibitor of 14-3-3-target protein interactions (R18), binding of both ß1Pix and Nedd4-2 to 14-3-3 proteins were reduced, and AMPK-dependent ENaC inhibition was also attenuated. Altogether, our results demonstrate the importance of AMPK-mediated phosphorylation of ß1Pix at Ser-71, which promotes 14-3-3 interactions with ß1Pix and Nedd4-2 to form a tripartite ENaC inhibitory complex, in the mechanism of ENaC regulation by AMPK.

13.
Braz. j. otorhinolaryngol. (Impr.) ; 85(5): 588-596, Sept.-Oct. 2019. tab, graf
Artigo em Inglês | LILACS-Express | ID: biblio-1039291

RESUMO

Abstract Introduction: There is still no general method for discriminating between benign and malignant leukoplakia and identifying vocal fold leukoplakia. Objective: To evaluate the reliability of a morphological classification and the correlation between morphological types and pathological grades of vocal fold leukoplakia. Methods: A total of 375 patients with vocal fold leukoplakia between 2009 and 2015 were retrospectively reviewed. Two observers divided the vocal fold leukoplakia into flat and smooth, elevated and smooth, and rough type on the basis of morphological appearance. The inter-observer reliability was evaluated and the results of classification from both observers were compared with final pathological grades. Clinical characteristics between low risk and high risk group were also analyzed. Results: The percentage inter-observer agreement of the morphological classification was 78.7% (κ = 0.615, p < 0.001). In the results from both observers, the morphological types were significantly correlated with the pathological grades (p1 < 0.001, p2 < 0.001, Kruskal-Wallis test; r1 = 0.646, p1 < 0.001, r2 = 0.539, p2 < 0.001, Spearman Correlation Analysis). Multivariate analysis showed patient's age (p = 0.018), the size of lesion (p < 0.001), and morphological type (p < 0.001) were significantly different between low risk group and high risk group. Combined receiver operating characteristic curve analysis of significant parameters revealed an area under the receiver operating characteristic curve of 0.863 (95% CI 0.823-0.903, p < 0.001). Conclusions: The proposed morphological classification of vocal fold leukoplakia was consistent between observers and morphological types correlated with pathological grades. Patient's age, the size of lesion, and morphological type might enable risk stratification and provide treatment guidelines for vocal fold leukoplakia.


Resumo Introdução: Ainda não há um método universal estabelecido para diferenciar entre a leucoplasia benigna e maligna ou identificar as leucoplasias das pregas vocais. Objetivo: Avaliar a confiabilidade de uma classificação morfológica e a correlação entre os tipos morfológicos e os graus histopatológicos das leucoplasias de pregas vocais. Método: Os registros de 375 pacientes com leucoplasia da prega vocal assistidos entre 2009 e 2015 foram revisados retrospectivamente. Dois observadores dividiram a leucoplasia da prega vocal entre tipo plano e liso, elevado e liso, e rugoso, com base na aparência morfológica. A confiabilidade interobservador foi avaliada e os resultados de classificação de ambos os observadores foram comparados com os graus histopatológicos finais. As características clínicas entre os grupos de baixo risco e alto risco também foram analisadas. Resultados: A porcentagem da concordância interobservador da classificação morfológica foi de 78,7% (κ = 0,615, p < 0,001). Nos resultados de ambos os observadores, os tipos morfológicos correlacionaram-se significativamente com os graus histopatológicos (p1 < 0,001, p2 < 0,001, teste de Kruskal-Wallis; r1 = 0,646, p1 < 0,001, r2 = 0,539, p2 < 0,001, análise de correlação de Spearman). A análise multivariada mostrou que a idade do paciente (p = 0,018), o tamanho da lesão (p < 0,001) e o tipo morfológico (p < 0,001) foram significativamente diferentes entre o grupo de baixo risco e o de alto risco. A análise da curva ROC (Receiver Operating Characteristic) combinada de parâmetros significativos revelou uma área sob a curva de 0,863 (IC 95%: 0,823 ± 0,903, p < 0,001). Conclusões: A classificação morfológica proposta para leucoplasia de prega vocal foi consistente entre observadores e os tipos morfológicos correlacionaram-se com os graus histopatológicos. A idade do paciente, o tamanho da lesão e o tipo morfológico podem permitir a estratificação de risco e fornecem diretrizes de tratamento para a leucoplasia da prega vocal.

14.
Biochem Biophys Res Commun ; 519(1): 198-203, 2019 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-31492500

RESUMO

A two-component regulatory system involving StyS and StyR is involved in the regulation of indigo synthesis in Pseudomonas sp. However, the function of the styR gene in indigo synthesis and the detailed mechanisms through which StyR enhances the expression of the styAB operon are unclear. Accordingly, in this study, we constructed a styR/styS gene knockout mutant strain. By comparing the differences in indigo yields between the wild-type and mutant strains, we found that the styR gene mutant strain had no indigo synthesis ability, whereas the yield in the wild-type strain was 5.4 mg/L. Thus, these findings indicate that the styR gene plays a key role in the regulation of indigo synthesis. The interactions among StyS, StyR, and the styAB promoter were verified by electrophoresis mobility shift assays. The results showed that StyR interacts with the styAB promoter by binding to the palindrome in the styAB promoter. Moreover, the kinase function of StyS regulated StyR by transphosphorylating StyR during indigo biosynthesis in P. putida B3. Taken together, these findings provide important insights into the establishment of environmentally friendly indigo synthesis methods using P. putida.

15.
Artigo em Inglês | MEDLINE | ID: mdl-31513009

RESUMO

A Gram-stain-negative, rod-shaped, chemoorganotrophic and anaerobic bacterium, strain SK-G1T, was isolated from oily sludge sampled at the Shengli oilfield in PR China. Growth occurred with 0-30 g l-1 NaCl, at 40-65 °C and at pH 6.0-8.5. The predominant fatty acids were C14 : 0 and C13 : 0, and the major cellular polar lipids were phosphatidylglycerol and phosphatidylethanolamine. No respiratory quinone was detected. The genomic G+C content was 43.9 mol%. The strain had highest 16S rRNA gene sequence similarity (93.2 % identity) to Tepidanaerobacter syntrophicus DSM 15584T. The phylogenetic, phenotypic and chemotaxonomic data showed that strain SK-G1T (=CCAM 530T=KCTC 15783T=JCM 33158T) represents a novel species of a new genus Biomaibacteracetigenes gen. nov., sp. nov. The results of phylogenetic and phylogenomic analyses indicated that the genera Biomaibacter, Caldanaerovirga, Fervidicola, Tepidanaerobacter, Thermosediminibacter, Thermovenabulum and Thermovoraxformed a clade with high bootstrap support distinguishing to other taxon within the order Thermoanaerobacterales. This clade is proposed as Thermosediminibacterales ord. nov. and includes Tepidanaerobacteraceae fam. nov. and Thermosediminibacteraceae fam. nov. Emended descriptions of the order Thermoanaerobacterales and family Syntrophomonadaceae are also provided.

16.
J Proteome Res ; 18(10): 3640-3648, 2019 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-31502464

RESUMO

Protein post-translational modification by the small ubiquitin-like modifier (SUMO) is a mechanism that allows a diverse response of cells to stress. Five SUMO family members, SUMO1-5, are expressed in mammals. We hypothesized that because kidney epithelial cells are often subject to stresses arising from various physiological conditions, multiple proteins in the kidney will be SUMOylated. Here, we profiled SUMO1- and SUMO2-modified proteins in a polarized epithelial cell model of the renal cortical collecting duct (mpkCCD14 cells). Modified forms of SUMO1 or SUMO2, with a histidine tag and a Thr to Lys mutation preceding the carboxyl-terminal di-gly motif, were expressed in mpkCCD14 cells, allowing SUMO-conjugated proteins to be purified and identified. Protein mass spectrometry identified 1428 SUMO1 and 1957 SUMO2 sites, corresponding to 741 SUMO1 and 971 SUMO2 proteins. Gene ontology indicated that the function of the majority of SUMOylated proteins in mpkCCD14 cells was related to gene transcription. After treatment of the mpkCCD14 cells for 24 h with aldosterone, the levels of SUMOylation at a specific site on the proton and oligopeptide/antibiotic cotransporter protein Pept2 were greatly increased. In conclusion, the SUMOylation landscape of mpkCCD14 cells suggests that protein modification by SUMOylation is a mechanism within renal epithelial cells to modulate gene transcription under various physiological conditions.

17.
J Breath Res ; 13(4): 046012, 2019 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-31489846

RESUMO

Breath analysis is used to detect the composition of exhaled gas. As a quick and non-invasive detection method, breath analysis provides deep insights into the progression of various kinds of diseases, especially those with metabolism disorders. Abundant information on volatile compounds in diabetic patients has been studied in numerous articles in the literature. However, exhaled gas in diabetic patients can be altered by various complications. So far, little attention has been paid to this alteration. In our paper, we found that under air pollution conditions, diabetic patients exhale more nitric oxide. Diabetic patients with heart failure exhale more acetone than those without heart failure. After 13C-labeled glucose intake, patients infected with Helicobacter pylori exhaled more 13C and less 18O than those without infection. Exhalation with chronic kidney disease changes volatile organic compounds on a large scale. Diabetic patients with ketoacidosis exhale more acetone than those without ketoacidosis. Some specific volatile organic compounds also emanate from diabetic feet. By monitoring breath frequency, diabetic patients with obstructive sleep apnea syndrome exhibit a unique breath pattern and rhythm as compared with other diabetic patients, and sleep apnea is prevalent among diabetic patients. In addition to clinical findings, we analyzed the underlying mechanisms at the levels of molecules, cells and whole bodies, and provided suggestions for further studies.

18.
Micron ; 126: 102738, 2019 11.
Artigo em Inglês | MEDLINE | ID: mdl-31476526

RESUMO

Single-barrel scanning electrochemical cell microscopy (SECCM) can be used to perform electrochemical activity analysis and sample surface imaging simultaneously. Compared to SECM & SICM in imaging, the most significant advantage of SECCM is that it does not need to immerse sample in solution, which avoids the electrochemical reaction between electrolyte and sample surface. In traditional direct current (DC) topographic imaging method of SECCM, when the meniscus droplet is contacted with the sample surface, the presence of the redox current determines the Z-height of a scanning point. However, there are some problems in DC mode. Firstly, the redox (Faraday) current is very small (pA/nA), which is susceptible to interference of ambient environment. Secondly, since the inertia of the droplet, the overall height of the imaged topography depends on the droplet size (probe tip diameter) and scanning speed. Therefore, this paper first proposes a single-barrel SECCM circuit model and verifies this circuit model using the first-order zero-state response in the DC mode. Then, an AC scanning mode is proposed, which monitors the change of AC amplitude to determine the Z-height of the scanning point when the meniscus droplet approaches the surface of the sample. The experiments demonstrate that the AC mode has a powerful ability to overcome interference and provide high-stable topographic imaging.

19.
Eur Arch Otorhinolaryngol ; 276(10): 2857-2864, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31367834

RESUMO

OBJECTIVE: To evaluate the predictive value of preoperative peripheral inflammatory markers in patients with vocal fold leukoplakia. METHODS: A retrospective study was performed of the patients diagnosed with vocal fold leukoplakia and who accepted carbon dioxide (CO2) laser resection in our center in the last 10 years. We calculated the neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR) and monocyte-to-lymphocyte ratio (MLR) after collecting and analyzing the clinical, histopathological and laboratory data. The potential relation between blood indexes and clinical events as recurrence or canceration was evaluated. RESULTS: A total of 589 patients were involved, including 300 cases without recurrence (group A), 198 with recurrence but not canceration (group B) and 91 transformed into squamous cancer (group C). Baseline analysis of NLR, PLR, and MLR showed no difference among the three groups before the first surgery. But all the indexes significantly elevated in groups B (P < 0.001, < 0.001, 0.023, respectively) and C (P = 0.009, 0.004, 0.007, respectively) in the last operation. The receiver-operating curve (ROC) analysis showed NLR as a potential marker of canceration of leukoplakia (AUC = 0.837) and the cutoff value was 2.505. When regrouping with pathological outcomes, severe dysplasia and squamous cell carcinoma (SCC) groups both revealed a higher level of NLR, PLR, and MLR comparing to the no dysplasia, mild dysplasia, and moderate dysplasia groups. NLR, PLR, and MLR in high-risk group (moderate, severe dysplasia and carcinoma) also elevated comparing to low-risk group (no dysplasia, mild dysplasia) (P = 0.039, 0.011, 0.007, respectively). CONCLUSIONS: The peripheral inflammatory markers NLR, PLR, and MLR are closely connected with the development of vocal fold leukoplakia. NLR may be a potential marker to predict the poor outcomes (recurrence or canceration) of patients in first surgery.

20.
J Colloid Interface Sci ; 555: 94-103, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31377648

RESUMO

Small plasmonic Au nanoparticles (NPs)-decorated with TiO2 nanosheets were fabricated to improve the photocatalytic performance. The Au/TiO2 nanosheets with Au NPs of different sizes ranging from ∼3 nm to 28 nm were prepared by using hydrothermally obtained TiO2 nanosheets as substrate via urea and light reduction method. During synthesis, the obtained Au NPs through urea reduction treatment in different calcination temperatures possessed smaller size (∼3-13 nm) than those of the light reduction method (∼28 nm). The introduced Au NPs were tightly loaded on the surface of TiO2 nanosheets through in situ growth reduction process of chloroauric acid. The emergence of smaller Au NPs promoted the photocatalytic performance over Au/TiO2 nanosheets. The as-prepared Au/TiO2 nanosheets with small Au NP sizes of ∼3-5 nm showed the highest photocatalytic rate of hydrogen production (∼230 µmol·h-1) under xenon lamp illumination, exceeding more than twice that of Au/TiO2 nanosheets with loading of larger Au NPs (∼28 nm). The favorable constituents and combination of Au/TiO2 nanosheets provided large surface adsorptive sites for reactant adsorption, introduced plasmonic effects and formed Schottky barrier junction via surface plasmon resonance. The Schottky barrier height was lower due to the presence of smaller Au NPs, thereby enhancing the charge separation through the Schottky transfer hub to neighboring TiO2 nanosheets. The synergistic effect between the plasmonic hot carrier-driven Au NPs and TiO2 nanosheets was discussed. The photocatalytic mechanism was also proposed for the fabrication of visible light-restricted photocatalysts with smaller Au NPs.

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