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1.
Sci Total Environ ; 711: 135098, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32000339

RESUMO

Diabetes is a major public health problem globally, and heat exposure may be a potential risk factor for death among diabetes. This study examines the association between heat and diabetes mortality in different regions of Thailand and investigates whether heat effects are modified by regional greenness. Daily temperature and daily diabetes deaths data were obtained for 60 provinces of Thailand during 2000-2008. A case-crossover analysis was conducted to quantify the odds of heat-related death among diabetes. Meta-regression was then used to examine potential modification effects of regional greenness (as represented by the Normalized Difference Vegetation Index) on heat-related mortality. A strong association between heat and diabetes mortality was found in Thailand, with important regional variations. Nationally, the pooled odds ratio of diabetes mortality was 1.10 (95% confidence interval (CI): 1.06-1.14) for heat (90th percentile of temperature) and 1.20 (95% CI: 1.10-1.30) for extreme heat (99th percentile of temperature) compared with the minimum mortality temperature, across lag 0-1 days. Central and northeast Thailand were the most vulnerable regions. Regional greenness modified the effects of heat, with lower mortality impacts in areas of higher levels of greenness. In conclusion, heat exposure increases mortality risk in diabetes, with large geographical variations in risk suggesting the need for region-specific public health strategies. Increasing greenness levels may help to reduce the burden of heat on diabetes in Thailand against the backdrop of a warming climate.

2.
Biomaterials ; 238: 119829, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32058868

RESUMO

Magnesium (Mg)-based metals can be used as next-generation fracture internal fixation devices due to their specific properties. We used vascularized bone grafting fixed by degradable pure Mg screws and obtained satisfactory results in the treatment of osteonecrosis of the femoral head. However, the mechanical properties of these screws make them weaker than those made of traditional metals. In particular, one of the main challenges of using screws made of Mg-based metals is their application in fixation at important weight-bearing sites in the human body. Femoral neck fracture is a common clinical injury. In this injury, the large bearing stress at the junction requires a fixation device with extremely high mechanical strength. Surgery and appropriate internal fixation can accelerate the healing of femoral neck fractures. Traditional internal fixation devices have some disadvantages after surgery, including stress shielding effects and the need for secondary surgery to remove screws. On the basis of previous work, we developed high-strength pure Mg screws for femoral neck fractures. In this study, we describe the first use of high-purity Mg to prepare large-size weight-bearing screws for the fixation of femoral neck fractures in goats. We then performed a 48 weeks follow-up study using in vivo transformation experiments. The results show that these biodegradable high-purity Mg weight-bearing screws had sufficient mechanical strength and a degradation rate compatible with bone repair. Furthermore, good bone formation was achieved during the degradation process and reconstruction of the bone tissue and blood supply of the femoral head and femoral neck. This study provides a basis for future research on the clinical transformation of biodegradable high-purity Mg weight-bearing screws.

3.
Sci Total Environ ; 708: 135096, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-31806312

RESUMO

Cadmium (Cd) is one of the most hazardous trace metals, and rapeseed is a major oil crop over the world with considerable lignocellulose residues applicable for trace metal phytoremediation and cellulosic ethanol co-production. In this study, we examined that two distinct rapeseed cultivars could accumulate Cd at 72.48 and 43.70 ug/g dry stalk, being the highest Cd accumulation among all major agricultural food crops as previously reported. The Cd accumulation significantly increased pectin deposition as a major factor for trace metal association with lignocellulose. Meanwhile, the Cd-accumulated rapeseed stalks contained much reduced wall polymers (hemicellulose, lignin) and cellulose degree of polymerization, leading to improved lignocellulose enzymatic hydrolysis. Notably, three optimal chemical pretreatments were performed for enhanced biomass enzymatic saccharification and bioethanol production by significantly increasing cellulose accessibility and lignocellulose porosity, along with a complete Cd release for collection and recycling. Hence, this study proposed a mechanism model interpreting why rapeseed stalks are able to accumulate much Cd and how the Cd-accumulated stalks are of enhanced biomass saccharification. It has also provided a powerful technology for both cost-effective Cd phytoremediation and value-added bioethanol co-production with minimum waste release.

4.
Surg Technol Int ; 35: 406-409, 2019 11 10.
Artigo em Inglês | MEDLINE | ID: mdl-31687779

RESUMO

Tantalum rod implantation with vascularized bone transplantation has been reported to be an effective method for the treatment of osteonecrosis of the femoral head (ONFH). However, long-term follow-up results were unclear. Sixty-five patients (71 hips) with ONFH treated with this technique were retrospectively reviewed. According to the Association Research Circulation Osseous (ARCO) classification, 21 hips were stage II, 30 were stage III, and 15 were stage IV. Sixty-one patients (66 hips) were followed for more than 10 years. Fifteen hips had to be converted to total hip arthroplasty (THA), the proportion of THA conversion surgery over 10 years postoperative was 4.76% for stage II, 16.7% for stage III, and 60% for stage IV, respectively. The 10-year joint-preserving success rate of the entire group was 77.2%. The Harris Hip Score (HHS) in the patients not receiving THA therapy increased from a mean of 51.35 points (ranged from 32 to 62 points) to 90.12 points (ranged from 72 to 99 points). The technique of tantalum rod implantation with vascularized bone grafting was an effective joint-preserving method for the treatment of ARCO stage II-III ONFH.


Assuntos
Transplante Ósseo , Necrose da Cabeça do Fêmur , Cabeça do Fêmur/cirurgia , Necrose da Cabeça do Fêmur/cirurgia , Seguimentos , Humanos , Estudos Retrospectivos , Análise de Sobrevida , Tantálio , Resultado do Tratamento
5.
BMC Med Res Methodol ; 19(1): 78, 2019 04 11.
Artigo em Inglês | MEDLINE | ID: mdl-30975073

RESUMO

BACKGROUND: To investigate the prevalence of protocol registration (or development) among published dose-response meta-analyses (DRMAs), and whether DRMAs with a protocol are better than those not. METHODS: Three databases were searched for eligible DRMAs. The modified AMSTAR (14 items) and PRISMA checklists (26 items) were used to assess the methodological and reporting quality, with each item assigned 1 point if it met the requirement or 0 if not. We matched (1,2) DRMAs with registered or published protocol to those not, by region and publication years. The summarized quality score and compliance rate of each item were compared between the two groups. Multivariable regression was employed to see if protocol registration or development was associated with total quality score. RESULTS: We included 529 DRMAs, with 45 (8.51%) completed protocol registration or development. We observed a higher methodological score for DRMAs with protocol than the matched controls (9.47 versus 8.58, P <  0.01); this embodied in 4 out of 14 items of AMSTAR [e.g., Duplicate data extraction (rate difference, RD = 0.17, 95% CI: 0.04, 0.30; P = 0.01). A higher reporting score (cubic transformed) for DRMAs with protocol than the matched controls was also observed (11,875.00 versus 10,229.53, P <  0.01); which embodied in 6 out of 26 items of PRISMA [e.g. Describe methods for publication bias (RD = 0.08, 95% CI: 0.01, 0.14; P = 0.02)]. Regression analysis suggested positive association between protocol registration or development and total reporting score (P = 0.012) while not for methodological score (P = 0.87). CONCLUSIONS: Only a small proportion of DRMAs completed protocol registration or development, and those with protocol were better reported than those not. Protocol registration or development is highly desirable.

6.
Stem Cell Res Ther ; 10(1): 72, 2019 03 05.
Artigo em Inglês | MEDLINE | ID: mdl-30837004

RESUMO

BACKGROUND: The body is unable to repair and regenerate large area bone defects. Moreover, the repair capacity of articular cartilage is very limited. There has long been a lack of effective treatments for osteochondral lesions. The engineered tissue with biphase synthetic for osteochondral repair has become one of the hot research fields over the past few years. In this study, an integrated biomanufacturing platform was constructed with bone marrow mesenchymal stem cells (BMSCs)/porous tantalum (pTa) associated with chondrocytes/collagen membranes (CM) to repair large osteochondral defects in load-bearing areas of goats. METHODS: Twenty-four goats with a large osteochondral defect in the femoral heads of the left hind legs were randomly divided into three groups: eight were treated with chondrocytes/CM-BMSCs/pTa, eight were treated with pure CM-pTa composite, and the other eight goats were untreated. The repair effect was assessed by X-ray, gross observation, and histomorphology for 16 weeks after the operation. In addition, the biocompatibility of chondrocytes/CM-BMSCs/pTa was observed by flow cytometry, CCK8, immunocytochemistry, and Q-PCR. The characteristics of the chondrocytes/CM-BMSCs/pTa were evaluated using both scanning electron microscopy and mechanical testing machine. RESULTS: The integrated repair material consists of pTa, injectable fibrin sealant, and CM promoted adhesion and growth of BMSCs and chondrocytes. pTa played an important role in promoting the differentiation of BMSCs into osteoblasts. Three-dimensional CM maintained the phenotype of chondrocytes successfully and expressed chondrogenic genes highly. The in vivo study showed that after 16 weeks from implantation, osteochondral defects in almost half of the femoral heads had been successfully repaired by BMSC-loaded pTa associated with biomimetic 3D collagen-based scaffold. CONCLUSIONS: The chondrocytes/CM-BMSCs/pTa demonstrated significant therapeutic efficacy in goat models of large osteochondral defect. This provides a novel therapeutic strategy for large osteochondral lesions in load-bearing areas caused by severe injury, necrosis, infection, degeneration, and tumor resection with a high profile of safety, effectiveness, and simplicity.

7.
PeerJ ; 7: e6295, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30697486

RESUMO

Objective: In evidence synthesis practice, dealing with binary rare adverse events (AEs) is a challenging problem. The pooled estimates for rare AEs through traditional inverse variance (IV), Mantel-Haenszel (MH), and Yusuf-Peto (Peto) methods are suboptimal, as the biases tend to be large. We proposed the "one-stage" approach based on multilevel variance component logistic regression (MVCL) to handle this problem. Methods: We used simulations to generate trials of individual participant data (IPD) with a series of predefined parameters. We compared the performance of the MVCL "one-stage" approach and the five classical methods (fixed/random effect IV, fixed/random effect MH, and Peto) for rare binary AEs under different scenarios, which included different sample size setting rules, effect sizes, between-study heterogeneity, and numbers of studies in each meta-analysis. The percentage bias, mean square error (MSE), coverage probability, and average width of the 95% confidence intervals were used as performance indicators. Results: We set 52 scenarios and each scenario was simulated 1,000 times. Under the rule of three (a sample size setting rule to ensure a 95% chance of detecting at least one AE case), the MVCL "one-stage" IPD method had the lowest percentage bias in most of the situations and the bias remained at a very low level (<10%), when compared to IV, MH, and Peto methods. In addition, the MVCL "one-stage" IPD method generally had the lowest MSE and the narrowest average width of 95% confidence intervals. However, it did not show better coverage probability over the other five methods. Conclusions: The MVCL "one-stage" IPD meta-analysis is a useful method to handle binary rare events and superior compared to traditional methods under the rule of three. Further meta-analyses may take account of the "one-stage" IPD method for pooling rare event data.

8.
J Clin Epidemiol ; 107: 1-11, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30445166

RESUMO

OBJECTIVES: To investigate methodological quality of published dose-response meta-analysis (DRMA) and explore study characteristics associated with the quality. STUDY DESIGN AND SETTING: We searched three databases for published DRMAs and used a modified AMSTAR (15 items) checklist to assess the methodological quality. We summarized the compliance of those DRMAs to the AMSTAR items and used multivariable regression analysis to explore the association between prespecified study characteristics with the overall methodological quality. RESULTS: We identified 529 DRMAs. Of the methodological quality items, six were well complied (80% or more) and six poorly complied (30% or fewer) by these DRMAs. The median score was nine points [first and third quartile: 7, 10] and only 64/529 had score over 10 points. Regression analysis suggested that studies with more authors (ß = 0.19; 95% confidence interval [CI]: 0.05, 0.33), published more recently (ß = 0.29; 95% CI: 0.21, 0.36), with financial support (ß = 0.41; 95% CI: 0.13, 0.70), conducted by authors from European (other regions vs. European, ß = -0.68; 95% CI: -1.05, -0.31) were associated with better methodological quality. CONCLUSION: The methodological quality of published DRMAs was suboptimal. Substantial efforts are warranted to improve the quality, including developing methodology guideline, involving more methodological trained authors, and so forth.

9.
BMC Med Res Methodol ; 18(1): 157, 2018 11 29.
Artigo em Inglês | MEDLINE | ID: mdl-30497389

RESUMO

BACKGROUND: Dose-response meta-analysis (DRMA) is a useful tool to investigate potential dose-response relationship between certain exposure or intervention and the outcome of interest. A large number of DRMAs have been published in the past several years. However, the standard of reporting for such studies is not known. METHODS: Medline, Embase, and Wiley Library were searched for systematic reviews with DRMAs (SR-DRMAs) published from January 2011 to July 2017. We used the combination of PRISMA and MOOSE statements, containing 33 items, to assess the reporting of included SR-DRMAs. The adherence of reporting was defined as the proportion of SR-DRMAs meeting the reporting requirement of an item. We explored the association between five pre-specified variables with the total score of reporting on both fully as well as each domain of the checklist. RESULTS: In total, 529 SR-DRMAs were eligible. Ten out of 33 items were under reported, and this mainly refers to the methods domain: only a small proportion of SR-DRMAs stated whether a review protocol existed (45, 8.5%); clarified the qualifications of searchers (1.7%); presented full electronic search strategy (25.9%); described any effort to include all available studies (22.9%), described methods for languages other than English (27.4%), and stated the process for selecting studies (20.2%). Multiple regression analysis suggested that studies with more authors (regression coefficient = 0.78; 95% CI: 0.35, 1.20; P <  0.001), published more recently (regression coefficient = 0.38; 95% CI: 0.28 to 0.47; trend P <  0.001), used reporting guideline (regression coefficient = 0.98; 95% CI: 0.68 to 1.32; P <  0.001), and involvement of methodologist (regression coefficient = 0.86; 95% CI: 0.42 to 1.32; P <  0.001) were associated with higher score of reporting. Further regression suggested that the improvement on the quality mainly concentrated on the methods and results domains. CONCLUSIONS: The reporting of SR-DRMAs needs to be further improved, particularly in the issues refer to the methods. The quality of reporting may improve when involving more authors and methodologists and employing any reporting guidelines.


Assuntos
Metanálise como Assunto , Publicações Periódicas como Assunto/normas , Relatório de Pesquisa/normas , Revisão Sistemática como Assunto , Estudos Transversais , Confiabilidade dos Dados , Coleta de Dados/métodos , Coleta de Dados/normas , Relação Dose-Resposta a Droga , Humanos , /normas
10.
Medicine (Baltimore) ; 97(38): e12400, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30235709

RESUMO

The aim of this study was to investigate the relationship between femoroacetabular impingement (FAI) and superior retinacular artery interruption using plain radiographs and digital subtraction angiography (DSA).Sixty-one patients included in this study were divided into 2 groups based on the superior retinacular artery integrity as evaluated on DSA images. Group A included intact arteries: 33 patients (24 men, 9 women,); group B included interrupted arteries: 28 patients (21 men, 7 women). The parameters of abnormal radiographic findings thought to be associated with FAI, including positive crossover or figure-of-eight sign of acetabulum, lateral center edge angle (LCEA) >40°, Tönnis angle <0°, positive posterior wall sign, alpha angle >50°, and coxa profunda, were evaluated in all patients through plain radiographs.The cross-over sign (Group A: 0, Group B: 8, P = .0035), LCEA (Group A: 1, Group B: 7, P = .0190), Tönnis angle (Group A: 3, Group B: 13, P = .0026), and alpha angle (Group A: 7, Group B: 17, P = .0039) differed significantly between both groups. However, there were no statistically significant differences in posterior wall sign (Group A: 9, Group B: 12, P = .3143) or coxa profunda (Group A: 12, Group B: 8, P = .7096).Patients with interrupted blood supply of the superior retinacular arteries displayed more parameters of abnormal radiographic findings associated with FAI thereby indicating potential correlation between FAI and the interruption of superior retinacular arteries.


Assuntos
Angiografia Digital/métodos , Impacto Femoroacetabular/diagnóstico por imagem , Artéria Femoral/diagnóstico por imagem , Acetábulo/irrigação sanguínea , Acetábulo/diagnóstico por imagem , Adulto , Estudos Cross-Over , Feminino , Impacto Femoroacetabular/patologia , Artéria Femoral/patologia , Quadril/irrigação sanguínea , Quadril/diagnóstico por imagem , Humanos , Masculino , Pessoa de Meia-Idade , Radiografia/métodos , Estudos Retrospectivos , Adulto Jovem
11.
Int J Biol Macromol ; 116: 106-112, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29729342

RESUMO

The present study aimed to investigate the effects of a purified polysaccharide (SPS) from the safflower in a cellular model of steroid-associated necrosis of the femoral head (SANFH), which was established in primary murine osteoblasts suffering dexamethasone pretreatment. After treatment with SPS (25, 50 and 100 µg/ml), the degree of necrosis induced by dexamethasone was significantly reduced in osteoblasts as evidenced by an increase of cell viability and a decrease of apoptosis in osteoblasts. Furthermore, pretreatment with SPS (25, 50 and 100 µg/ml) significantly attenuated the activation of caspase-3 and cleavage of PARP relative to the model control cells. The addition of caspase-3 inhibitor (Z-DEVD-FMK) in dexamethasone-treated osteoblasts resulted in the inefficiency of SPS for inhibiting cellular apoptosis. Dose-dependent increases in alkaline phosphatase (ALP) activity, collagen synthesis and mineralization were also observed in SPS-treated osteoblasts at 72 h. The present study demonstrates that SPS may alleviate dexamethasone associated osteonecrosis by inhibiting caspsae-3-mediated apoptosis and may provide an alternative treatment for SANFH.


Assuntos
Apoptose/efeitos dos fármacos , Carthamus tinctorius/metabolismo , Caspase 3/metabolismo , Necrose da Cabeça do Fêmur/tratamento farmacológico , Polissacarídeos/farmacologia , Animais , Proteínas Reguladoras de Apoptose/metabolismo , Sobrevivência Celular/efeitos dos fármacos , Dexametasona/farmacologia , Necrose da Cabeça do Fêmur/induzido quimicamente , Necrose da Cabeça do Fêmur/metabolismo , Camundongos , Osteoblastos/efeitos dos fármacos , Ratos , Ratos Wistar , Transdução de Sinais/efeitos dos fármacos , Esteroides/farmacologia
12.
Bioresour Technol ; 263: 67-74, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29730520

RESUMO

In this study, total eight distinct Miscanthus accessions were collected from the cadmium (Cd)-supplied soil pots, and mild alkali pretreatments (0.5%, 1% NaOH) were then performed to enhance biomass enzymatic saccharification. Due to large Cd accumulation, all Miscanthus accessions showed significantly reduced cellulose levels and features (CrI, DP) with much increased hemicellulose and pectin contents in the mature stems. Under mild alkali pretreatments, all Miscanthus samples exhibited largely increased hexoses yields released from enzymatic hydrolysis, and one desirable accession had an almost complete biomass saccharification with the hexoses yield at 100% (% cellulose). Notably, the biomass residues remained from enzymatic hydrolysis upon 1% NaOH pretreatment could absorb 73-96% Cd (% of total), suggesting an applicable approach for Cd phyto-remediation. Hence, a hypothetic model was proposed to elucidate that the enhanced biomass saccharification should be mainly due to much reduced cellulose CrI and DP in the Cd-accumulated Miscanthus accessions.


Assuntos
Cádmio/isolamento & purificação , Polissacarídeos/metabolismo , Biomassa , Cádmio/farmacocinética , Celulose , Hidrólise , Lignina , Pectinas
13.
Orthop Surg ; 10(1): 69-74, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29424129

RESUMO

The present study investigates the feasibility of micro perfusion of femoral head specimens from femoral neck fracture patients by the inferior retinacular arteries and performing intraosseous artery quantitative analysis of the femoral head. Twelve femoral neck fracture patients who had undergone conventional hip replacement surgery were included in this study. Femoral head specimen arteries were first dissected and exposed and then perfused by the inferior retinacular arteries and all the femoral heads underwent micro-CT scanning. After micro-CT scanning, a digital 3-D model was reconstructed to quantify the femoral head intraosseous arteries for comparison with a normal femoral head. The artery length density, artery volume density, and artery length/volume ratio were calculated separately and compared with normal femoral head parameters. Micro-CT scanning displayed the epiphyseal arterial network structure and their fine vascular branches in all 12 femoral neck fractures. Blood was supplied from the inferior retinacular artery to the epiphyseal arterial network then to all the fine blood vessels within the femoral head. No statistical differences were observed in femoral heads' intraosseous artery length densities or volume densities between the normal and femoral neck fracture specimens, while the artery length/volume ratio showed a statistical difference, and the ratio increased from 19 to 46. Micro perfusion of the femoral head by the inferior retinacular arteries is possible and can present the epiphyseal network and their fine arterial branches in pathologic conditions to provide a morphological basis for the study of femoral head disease.


Assuntos
Fraturas do Colo Femoral/patologia , Cabeça do Fêmur/irrigação sanguínea , Adulto , Idoso , Idoso de 80 Anos ou mais , Artérias/diagnóstico por imagem , Artérias/patologia , Artroplastia de Quadril , Meios de Contraste , Estudos de Viabilidade , Feminino , Fraturas do Colo Femoral/diagnóstico por imagem , Cabeça do Fêmur/diagnóstico por imagem , Humanos , Imagem Tridimensional/métodos , Masculino , Pessoa de Meia-Idade , Microtomografia por Raio-X
14.
Int J Clin Exp Med ; 8(8): 13259-66, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26550251

RESUMO

Several studies reported Prostate stem cell antigen (PSCA) rs2294008 was susceptibly associated with bladder cancer (BC) risk. However, the results were not entirely consistent. The aim of this study was to investigate the association between rs2294008 and BC risk. Comprehensive meta-analysis was preformed to provide a more precise assessment of the association between rs2294008 and BC risk. Twenty five studies involving 14,244 BC patients and 53,963 controls were included in our meta-analysis. The crude odds ratios (ORs) and the 95% confidence intervals (95% CIs) were used to evaluate the strength of the association. Pooled results indicated that the PSCA variant rs2294008-T was significantly connected with an increased risk of BC (OR = 1.15, 95% CI = 1.12-1.18, P(z) < 0.0001). Moreover, stratified analyses showed that rs2294008 significantly increased BC risk in European (OR = 1.10, 95% CI = 1.05-1.15, P(z) < 0.0001), North American (OR = 1.18, 95% CI = 1.12-1.24, P(z) < 0.0001), and Asian (OR = 1.17, 95% CI = 1.13-1.22, P(z) < 0.0001). In conclusion, our meta-analysis demonstrated that the PSCA rs2294008 is a risk factor for BC in European, Asian and North American. Further large case-control studies are needed to assess the relationship in other populations. Biologically functional studies are needed to verify the molecular mechanisms in the pathogenesis of BC.

15.
Zhongguo Zhong Xi Yi Jie He Za Zhi ; 28(3): 229-33, 2008 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-18476423

RESUMO

OBJECTIVE: To observe the effect of Bushen Antai Recipe (BAR) on expression of prostaglandin I2 (PGI2) and its nuclear receptor peroxisome proliferators-activated receptor delta (PPARdelta) at implantation site in mice with blastocyst implantation dysfunction. METHODS: Pregnant mice were divided into three groups randomly, the normal group, the model group and the BAR group. The pregnant uterus of all mice was cut off on the 4th (D4), 5th (D5), 6th (D6) and 8th (D8) day of pregnancy for determining the PGI2 expression with radio immunoassay; and the mRNA and protein expression of PPARdelta with RT-PCR and immunohistochemistry at implantation site. RESULTS: PGI2 expression in the model group was obviously lower than that in the normal group (P < 0.01), and also lower than that in the BAR group (P < 0.01), while the index was insignificantly different between the normal and the BAR group. Compared with the normal group, the expression of PPARdelta in the model group was delayed temporally and spatially (P < 0.05), while that in the BAR group was not significantly different. CONCLUSION: BAR can improve the implantation in mice with blastocyst implantation dysfunction through promoting the PGI2 expression and its nuclear receptor PPARdelta at implantation site.


Assuntos
Medicamentos de Ervas Chinesas/farmacologia , Implantação Tardia do Embrião/efeitos dos fármacos , Epoprostenol/biossíntese , PPAR delta/biossíntese , Animais , Endométrio/efeitos dos fármacos , Endométrio/metabolismo , Feminino , Imuno-Histoquímica , Masculino , Camundongos , PPAR delta/genética , Gravidez , RNA Mensageiro/biossíntese , RNA Mensageiro/genética , Radioimunoensaio , Reação em Cadeia da Polimerase Via Transcriptase Reversa
16.
Artigo em Chinês | MEDLINE | ID: mdl-18365625

RESUMO

OBJECTIVE: To explore the short-term clinical effects on application of absorbable RIGIDfix cross pins and Intrafix screw for posterior cruciate ligament (PCL) reconstruction with autogenetic or xenogenic semitendinosus-plus-semimembranosus tendons under arthroscope. METHODS: From June 2005 to January 2007, 36 cases of PCL injuries (including 20 fresh injuries and 16 old ones) were treated. There were 21 males and 15 females, aged 15-51 years old, mean 30.4 years. There were 19 cases in the left side and 17 cases in the right side. Thirteen cases out of them complicated with medial collateral ligament injury; 9 cases complicated with lateral collateral ligament injury; 2 cases complicated with posterolateral structure injury; 11 cases complicated with medial meniscus injury; 10 cases complicated with lateral meniscus injury; 2 cases complicated with nerve and vascular injury. PCL reconstruction was accomplished by arthroscopy with autogenetic or xenogenic 4-strand semitendinosus-plus-semimembranosus tendons. RIGIDfix cross pins were used for tibial side fixation, and Intrafix was used for femoral side fixation. Lysholm scores, international knee documentation(IKDC) grading and Lachman experiment were used for evaluation before operation and in the follow up. RESULTS: All the cases had been followed up for 6 to 26 months (average 10.4 months). IKDC grading showed that 3 patients (8.4 %) were graded as B, 12 patients (33.3%) as C, 21 patients (58.3%) as D before operation, while 9 patients (25 %) were graded as A, 21 patients (58.3%) as B, 6 patients (16.7%) as C after operation. Lysholm scores raised from 42.52 +/- 5.24 before operation to 91.24 +/- 5.68 after operation. Lachman experiment results showed that there were 5 cases (+), 20 cases (++) and 11 cases (+++) before operation, while there were 2 cases (+) and 34 cases (-) after operation. Each index was significant between before and after surgery (P < 0.05). After surgery, all the cases got good pain relief. Joint was stable, and motion range recovered. No pin and screw loosening was found. Only 4 cases suffered from effusion and synovitis after surgery, and they were recovered after conservative treatment. CONCLUSION: The method of using RIGIDfix-Intrafix complexes under arthroscopy for PCL reconstruction is simple and the fixation is rigid. Early functional exercise after operation is possible.


Assuntos
Pinos Ortopédicos , Parafusos Ósseos , Traumatismos do Joelho/cirurgia , Ligamentos Articulares/lesões , Ligamento Cruzado Posterior/cirurgia , Procedimentos Cirúrgicos Reconstrutivos/métodos , Tendões/transplante , Implantes Absorvíveis , Adolescente , Adulto , Artroscopia , Feminino , Seguimentos , Humanos , Instabilidade Articular/cirurgia , Traumatismos do Joelho/reabilitação , Ligamentos Articulares/cirurgia , Masculino , Pessoa de Meia-Idade , Ligamento Cruzado Posterior/lesões , Amplitude de Movimento Articular , Procedimentos Cirúrgicos Reconstrutivos/instrumentação , Resultado do Tratamento , Adulto Jovem
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