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1.
Nat Prod Res ; : 1-6, 2019 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-31617784

RESUMO

A new diketopiperazine cyclo-(L-Phe-N-ethyl-L-Glu) (1), along with two known diketopiperazines cyclo-(L-Pro-L-Leu) (2) and cyclo-(L-Pro-L-Phe) (3) were isolated from the cultures of an endophytic fungus Aspergillus aculeatus F027. The structures of these compounds were elucidated based on spectroscopic data. The configurations of these compounds were determined by advanced Marfey's analysis. Antibacterial activity of the diketopiperazines against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were also evaluated.

2.
MBio ; 10(5)2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31575768

RESUMO

Group A streptococcus (GAS) is an important human pathogen which can cause fatal diseases after invasion into the bloodstream. Although antibiotics and immune surveillance are the main defenses against GAS infection, GAS utilizes internalization into cells as a major immune evasion strategy. Our previous findings revealed that light chain 3 (LC3)-associated single membrane GAS-containing vacuoles in endothelial cells are compromised for bacterial clearance due to insufficient acidification after fusion with lysosomes. However, the characteristics and the activation mechanisms of these LC3-positive compartments are still largely unknown. In the present study, we demonstrated that the LC3-positive GAS is surrounded by single membrane and colocalizes with NADPH oxidase 2 (NOX2) complex but without ULK1, which are characteristics of LC3-associated phagocytosis (LAP). Inhibition of NOX2 or reactive oxygen species (ROS) significantly reduces GAS multiplication and enhances autolysosome acidification in endothelial cells through converting LAP to conventional xenophagy, which is revealed by enhancement of ULK1 recruitment, attenuation of p70s6k phosphorylation, and formation of the isolation membrane. We also clarify that the inactivation of mTORC1, which is the initiation signal of autophagy, is inhibited by NOX2- and ROS-activated phosphatidylinositol 3-kinase (PI3K)/AKT and MEK/extracellular signal-regulated kinase (ERK) pathways. In addition, streptolysin O (SLO) of GAS is identified as a crucial inducer of ROS for ß1 integrin-mediated LAP induction. After downregulation of ß1 integrin, GAS multiplication is reduced, accompanied with LAP inhibition and xenophagy induction. These results demonstrate that GAS infection preferentially induces ineffective LAP to evade xenophagic killing in endothelial cells through the SLO/ß1 integrin/NOX2/ROS pathway.IMPORTANCE Our previous reports showed that the LC3-associated GAS-containing single membrane vacuoles are inefficient for bacterial clearance in endothelial cells, which may result in bacteremia. However, the characteristics and the induction mechanisms of these LC3-positive vacuoles are still largely unknown. Here we provide the first evidence that these LC3-positive GAS-containing single membrane compartments appear to be LAPosomes, which are induced by NOX2 and ROS. Through NOX2- and ROS-mediated signaling, GAS preferentially induces LAP and inhibits bacteriostatic xenophagy in endothelial cells. We also provide the first demonstration that ß1 integrin acts as the receptor for LAP induction through GAS-produced SLO stimulation in endothelial cells. Our findings reveal the underlying mechanisms of LAP induction and autophagy evasion for GAS multiplication in endothelial cells.

4.
Adv Mater ; : e1904163, 2019 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-31592566

RESUMO

Light-emitting diodes (LEDs) based on solution-processed metal halide perovskites have shown great application potential in energy-efficient lighting and displays. Multiple-quantum-well (MQW) perovskites simultaneously possess high photoluminescence quantum efficiency and good film morphology and stability, making it attractive for high-performance perovskite LEDs. Here, merits of MQW perovskites and the progress in MQW perovskite LEDs are reviewed. Challenges and future directions of perovskite LEDs are also discussed.

5.
PLoS One ; 14(9): e0222684, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31545836

RESUMO

The improved split Hopkinson pressure bar (SHPB) was used to study the dynamic mechanical properties and failure characteristics of surrounding rock in deep rock mass engineering that is under high stress and affected by blasting excavation and other dynamic disturbances. In a three-dimensional high static load and frequent dynamic disturbance test, the preload high axial pressure and confining pressure are used to simulate the high crustal stress of deep rock, and the effect of small disturbances on the rock is simulated by the low impact load. The results show that there are two types of dynamic stress-strain curves of deep rock: an elastic-plastic curve and plastic-elastic-plastic curve. The curves consists of five parts: the compaction stage, micro-crack steady development stage, micro-crack unstable propagation stage, fatigue damage stage, and fatigue failure stage. Reductive phenomena of constringent strain after dynamic peak stress appear because of the different degrees of rock damage. Moreover, these phenomena include two conditions, namely, whether rebound occurs or not. The impact resistance of rock is strongest when the ratio of the confining pressure to axial pressure is optimal, and the dynamic average strength of rock and accumulative impact times decrease with the increase of the preloaded axial compression and increase with the increase of the preloaded confining pressure. Both the dynamic deformation modulus and dynamic peak stress decrease with the increase of the accumulative impact time, while the maximum strain and the dynamic peak strain increase. The corresponding rebound strain as a whole first increases and then decreases with the increasing impact times. For deep rock, tensile failure and single-bevel plane shear failure are the main failure modes, and pull-compression mixed friction failure is the auxiliary failure mode. Additionally, the lumpiness of broken rock decreases with the increase of the preloaded axial compression and increases with the increase of the preloaded confining pressure.

6.
FASEB J ; : fj201901161R, 2019 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-31545913

RESUMO

Endothelial glycocalyx plays a significant role in the development and progression of diabetic complications. Endomucin (EMCN) is an anti-inflammatory membrane glycoprotein that is mainly expressed in venous and capillary endothelial cells. However, the function of EMCN in diabetic retinopathy (DR) progression is still completely unknown. We first investigated the change of EMCN expression in the retina and human retinal microvascular endothelial cells. We then overexpressed EMCN in the retina with adeno-associated virus and induced DR with streptozotocin (STZ). We analyzed EMCN's effect on the integrity of endothelial glycocalyx under conditions of DR. Furthermore, we investigated EMCN's protective effect against inflammation and blood-retinal barrier (BRB) destruction. We found that EMCN is specifically expressed in retinal endothelial cells and that its levels are decreased during hyperglycemia in vitro and in vivo. Overexpression of EMCN can restore the retinal endothelial glycocalyx of STZ-induced diabetic rats. Furthermore, EMCN overexpression can decrease leukocyte-endothelial adhesion to ameliorate inflammation and stabilize the BRB to inhibit vessel leakage in rats with DR. EMCN may protect patients with diabetes from retinal vascular degeneration by restoring the endothelial glycocalyx. EMCN may thus represent a novel therapeutic strategy for DR because it targets endothelial glycocalyx degradation associated with this disease.-Niu, T., Zhao, M., Jiang, Y., Xing, X., Shi, X., Cheng, L., Jin, H., Liu, K. Endomucin restores depleted endothelial glycocalyx in the retinas of streptozotocin-induced diabetic rats.

7.
Toxicol Appl Pharmacol ; 382: 114703, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31398421

RESUMO

Pulmonary fibrosis (PF) is a fatal and irreversible lung disease that eventually causes respiratory failure, lung dysfunction and death. The peptide DHNNPQIR-NH2 (DR8) has been reported to possess potent antioxidant activity, and an imbalance of oxidation/antioxidation is a crucial mechanism that causes PF. Here, we studied the ability of DR8 to improve PF and further explored the pathway in which DR8 plays a critical role. We found that after prophylactic or therapeutic treatment with DR8, fibrosis-associated indices, including marker proteins, proinflammatory cytokines and profibrogenic cytokines, were significantly downregulated. Importantly, DR8 could reduce bleomycin-induced pathological changes and collagen deposition, especially collagen I content. Furthermore, DR8 prominently upregulated nonenzymatic antioxidants and enzymatic antioxidants. Consistent with the in vivo results, we observed that DR8 significantly inhibited the proliferation and reactive oxygen species (ROS) generation of A549 cells and NIH3T3 cells stimulated with transforming growth factor-ß1 (TGF-ß1), as well as decreased NADPH oxidase 4 (NOX4) levels under the same conditions. Moreover, DR8 reversed the TGF-ß1-induced upregulation of phosphorylated ERK1/2 and p38 MAPK in cells and the bleomycin-induced upregulation of these indices in mice. Our results indicate that DR8 could prevent and treat PF by reducing oxidative damage and suppressing the TGF-ß/MAPK pathway. Because of the high efficiency and low toxicity of DR8, we consider that DR8 could be a candidate drug for PF, and our studies establish a foundation for the development of a lead compound to be used as a therapy for fibrosis-related diseases.

8.
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue ; 31(7): 878-883, 2019 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-31441414

RESUMO

OBJECTIVE: To compare the influences of extracorporeal cardiopulmonary resuscitation (ECPR) and conventional or mechanical cardiopulmonary resuscitation (CCPR/MCPR) on survival rate and neurological outcome for adult patients with out-of-hospital cardiac arrest (OHCA), and to assess the effect of ECPR. METHODS: Databases such as Medline, Embase, ScienceDirect, HighWire, Cochrane Library, Wanfang Database and China National Knowledge Infrastructure (CNKI) were searched from January 2000 to October 2018 to retrieve clinical trials on comparison of the effect of ECPR and CCPR/MCPR on survival rate and neurological outcome of adult patients with OHCA. Thereafter, the studies retrieved were based on predefined inclusion and exclusion criteria. Data were extracted and the quality of the included studies was evaluated by two researchers. A meta-analysis was performed by using RevMan 5.3 software. Sensitivity analysis was used to evaluate the stability of the results, and funnel plot was used to evaluate publication bias. RESULTS: A total of 12 studies and 2 519 patients were enrolled, including 615 patients receiving ECPR and 1 904 patients receiving CCPR/MCPR. Meta-analysis showed that compared with CCPR/MCPR, ECPR could not improve the short-term (at hospital discharge or within 1 month) survival rate in patients with OHCA [odds ratio (OR) = 2.26, 95% confidence interval (95%CI) = 0.95-5.41, P = 0.07], but could increase long-term (at more than 3 months) survival rate (OR = 3.56, 95%CI = 1.65-7.71, P = 0.001), rate of good neurological outcome at hospital discharge [Glasgow-Pittsburgh cerebral performance categories (CPC) 1-2 was defined as good neurological function; OR = 3.39, 95%CI = 1.73-6.62, P = 0.000 4], and rate of good long-term neurological outcome (OR = 3.45, 95%CI = 2.24-5.32, P < 0.000 01). Sensitivity analysis showed that the overall results did not change significantly, whether using fixed-effect model and random-effect model to analyze the differences of each effect index, or excluding one study with fewer than 50 subjects for data analysis, indicating that the results were more stable. The funnel plot suggested that there was no publication bias in the studies. But due to the small number of studies, the publication bias could not be excluded. CONCLUSIONS: ECPR could not improve the short-term survival rate at hospital discharge or within 1 month in patients with OHCA, but could increase long-term survival rate at more than 3 months, good neurological outcome at hospital discharge and long-term neurological outcome.


Assuntos
Reanimação Cardiopulmonar , Parada Cardíaca Extra-Hospitalar/epidemiologia , Adulto , China/epidemiologia , Humanos , Taxa de Sobrevida , Resultado do Tratamento
9.
J Food Biochem ; 43(8): e12954, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31368555

RESUMO

With the improvement of living standards, obesity has become a serious health problem all over the word. Currently, the methods and drugs for obesity treatment have some limitations and side effects. Flavonoids are active constituents with various biological activities, widely found in plants, and numerous studies have shown that flavonoids can inhibit obesity and related metabolism disorders effectively. This perspective reviews the recent progress in understanding the anti-obesity effects of flavonoids through modulating food intake, enzyme activities, nutrition absorption, adipogenesis and adipocyte lifecycle, thermogenesis, energy consumption, and intestinal microbiota. PRACTICAL APPLICATIONS: Natural bioactive substance flavonoids have anti-obesity property, which may play a role in anti-obesity drugs or functional food without any side effects. Flavonoids can inhibit weight gain directly or through their biologically active metabolites by various potential pathways. A better understanding of the modulatory effect and the mechanism of flavonoids on obesity will allow us to better utilize flavonoids in plants to treat obesity and related metabolic syndrome.

10.
Oral Oncol ; 96: 27-33, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31422210

RESUMO

OBJECTIVES: Esophageal second primary tumors (SPTs) in head and neck cancer (HNC) patients is not uncommon. The impact of image-enhanced endoscopy (IEE) screening for esophageal SPT on the outcome of HNC patients has not been well clarified. METHODS AND METHODS: Patients with malignancies of the head and neck region and esophagus were recruited from a hospital-based cancer registry between July 2000-December 2016. IEE screening included magnifying endoscopy with narrow-band imaging and chromoendoscopy with Lugol's solution. Biopsied specimens with revised Vienna classification categories 1 and 2 were defined as group I, and those with categories 3 to 5 were defined as group II. The Kaplan-Meier estimate and Cox regression model were used for survival analysis. RESULTS: Totally 1577 HNC and 501 esophageal cancer patients were enrolled. The 5-year overall survival (OS) rates of stage I/II HNC, stage III/IV HNC and esophageal cancer patients were 58%, 29%, and 8%, respectively (p < 0.01). The 5-year OS rate of HNC patients with negative IEE results was higher than that of HNC patients without IEE screening, followed by IEE screening groups I, II and esophageal cancer patients (44% vs. 39% vs. 35% vs. 11% vs. 8%, respectively, p for trend <0.01). Among advanced HNC patients, those who received IEE screening had a trend of better prognosis than those without screening (5-year OS rate of 31% vs. 28%, p = 0.17). CONCLUSIONS: IEE screening for esophageal SPTs is helpful in risk stratification and prognosis prediction for HNC patients. Routine IEE screening is recommended in HNC patients.

11.
J Food Biochem ; 43(7): e12880, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31353696

RESUMO

Despite the remarkable bioactivity, the potential of EGCG3''Me to be fully utilized has not yet been completely elucidated due to its low absorption. It has been reported that phospholipids can act as agents to improve the absorption of antioxidants. Therefore, EGCG3''Me phospholipid complex (EPC) was utilized in this study to investigate its activity on gut flora of an obesity mouse model. After the administration of the complex for 8 weeks, the relative abundance of Bacteroidetes was significantly increased (p < 0.05); meanwhile, the relative abundance of Firmicutes was decreased, suggesting the potential anti-obesity effect of the complex. Furthermore, the expression of Muc2 and Reg3g were directly upregulated by EPC intervention. PRACTICAL APPLICATIONS: Although EGCG3''Me has shown excellent biological benefits, the presence of multiple hydroxyl groups and high polar properties hindered its application. This study indicated the potential of phospholipids in promoting the bioavailability of EGCG3''Me and might contribute to the production of functional food with better tea catechins absorption.

12.
Curr Med Chem ; 2019 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-31161985

RESUMO

Toll-like receptors (TLRs) are members of pattern recognition receptors and serve pivotal role in host immunity. TLRs response to pathogen-associated molecular patterns encoded by pathogens or damage-associated molecular patterns released by dying cells, initiating an inflammatory cascade, where both beneficial and detrimental effects can be exerted. Accumulated evidence has revealed TLRs tightly associate with various kidney diseases but their roles are still not well understood. This review updated evidence on roles of TLRs in the pathogenesis of kidney diseases including urinary tract infection, glomerulonephritis, acute kidney injury, transplant allograft dysfunction and chronic kidney diseases.

13.
Nat Commun ; 10(1): 2425, 2019 06 03.
Artigo em Inglês | MEDLINE | ID: mdl-31160563

RESUMO

It is a long-standing challenge to achieve efficient and highly selective aerobic oxidation of methylarenes to benzaldehydes, owing to overoxidation problem stemming from the oxidizability of benzaldehyde far higher than the toluene under usual aerobic conditions. Herein we report a bio-inspired iron-catalyzed polymethylhydrosiloxane-promoted aerobic oxidation of methylarenes to benzaldehydes with high yields and selectivities. Notably, this method can tolerate oxidation-labile and reactive boronic acid group, which is normally required to be transformed immediately after its introduction, and represents a significant advance in the area of the chemistry of organoboronic acids, including the ability to incorporate both aldehyde and ketone functionalities into unprotected arylboronic acids, a class that can be difficult to access by current means. The robustness of this protocol is demonstrated on the late-stage oxidation of complex bioactive molecules, including dehydroabietic acid, Gemfibrozil, Tocopherol nicotinate, a complex polyol structure, and structurally complex arylboronic acids.

14.
Ying Yong Sheng Tai Xue Bao ; 30(5): 1679-1686, 2019 May.
Artigo em Chinês | MEDLINE | ID: mdl-31107025

RESUMO

We investigated the growth traits (tree height, diameter at the breast, crown diameter), fruiting traits (total number of cones in 7 consecutive fruiting years) and resistance to disease and insect of 551 half-sib families of Pinus koraiensis superior trees in 29-year-old in Hongwei seed orchard of Lushuihe, Jilin Province, with the method of multi-trait comprehensive evaluation and combining with six traits. The results showed that all the traits were significantly different among different families or blocks. Phenotypic variation coefficient of different traits ranged from 13.9% to 61.0%. The extremely significant difference and high variation coefficients were beneficial for elite families evaluation and selection. The family heritability of volume, seed yield and resistance to disease and insect (the values were 0.47, 0.52, 0.48, respectively) were higher than single plant heritability (the values were 0.37, 0.07, 0.10, respectively). There was a extremely significant positive correlation between growth traits, fruiting traits and resistance to disease and insect. 28 elite families were selected with a selection rate of 5%, with the genetic gains of volume, seed yield and resistance to disease and insect being 16.8%, 71.4% and 0.5%, respectively. Seven elite individuals were selected from the elite families with a selection rate of 2%, with the genetic gains of volume, seed yield and resistance to disease and insect being 66.8%, 80.9% and 0.7%, respectively. These elite families and individual plants showed obvious advantages, which could guide the thinning of clonal seed orchards and provide breeding materials for the construction of high-generation seed orchards.


Assuntos
Adaptação Fisiológica , Pinus/fisiologia , Animais , Variação Genética , Insetos , Fenótipo , Melhoramento Vegetal , Árvores
15.
J Mol Model ; 25(6): 164, 2019 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-31101992

RESUMO

Density functional theory (DFT) has been employed within the generalized gradient approximation and Perdew-Burke-Ernzerhof functional (GGA-PBE) to study the structural and electronic properties of nitromethane (NM) surface models. Different surfaces, including (100), (001), (101), (110), and (111), are considered in this work. The corresponding properties of bulk crystal for NM were also calculated to form a contrast to the slab models. Results with anisotropic characteristics of different surfaces have been observed in this study. There was an obviously great anisotropy in electronic parameters, especially the band gaps of different surfaces, indicating the anisotropic impact sensitivity along different directions of NM. The band gap value for (111) surface, 2.687 eV, was smaller than that of other surfaces, showing a higher impact sensitivity for NM. The estimated anisotropy has been revealed in surface energies for different surfaces. Graphical Abstract The valence band minimum (VBM) and conduction band maximum (CBM) of the nitromethane (100), (001), (101), (110) and (111) surface models.

16.
Crit Care ; 23(1): 168, 2019 05 14.
Artigo em Inglês | MEDLINE | ID: mdl-31088524

RESUMO

BACKGROUND: Catecholamines, especially norepinephrine, are the most frequently used vasopressors for treating patients with septic shock. During the recent decades, terlipressin, vasopressin V1A agonist, and even Ca2+ sensitizer were increasingly used by physicians. The aim of this study is to compare the efficacy of such different kinds of vasoactive medications on mortality among patients with septic shock. METHODS: Relevant randomized controlled trials were identified by searching PubMed, Embase, Web of Science, and the Cochrane Central Register of Controlled Trials updated to February 22, 2018. A network meta-analysis was performed to evaluate the effect of different types of vasoactive medications. The primary outcome was 28-day mortality. Intensive care unit (ICU) mortality, hospital and ICU length of stay (LOS), and adverse events were also assessed. RESULTS: A total of 43 trials with 5767 patients assessing 17 treatment modalities were included. Treatments ranking based on surface under the cumulative ranking curve values from largest to smallest were NE/DB 85.9%, TP 75.1%, NE/EP 74.6%, PI 74.1%, EP 72.5%, VP 66.1%, NE 59.8%, PE 53.0%, DA 42.1%, DX 38.2%, SP 27.0%, PA 24.3%, EX 22.8%, LE 21.5%, and DB 13.3% for 28-day mortality. Treatments ranking for ICU mortality were TP/NE 86.4%, TP 80.3%, TP/DB/NE 65.7%, VP/NE 62.8%, NE 57.4%, VP 56.5%, PE 48.4%, DA 33.0%, PA 27.5%, LE 22.1%, and DB 9.9%. The incidence of myocardial infarction was reported with NE/EP 3.33% (n = 1 of 30), followed by EP 3.11% (n = 5 of 161), and then VP 3.10% (n = 19 of 613), NE 3.03% (n = 43 of 1417), DA 2.21% (n = 19 of 858), NE/DB 2.01% (n = 4 of 199), LE 1.16% (n = 3 of 258), and PA 0.39% (n = 1 of 257). The incidence of arrhythmia was reported with DA 26.01% (n = 258 of 992), followed by EP 22.98% (n = 37 of 161), and then NE/DB 20.60% (n = 41 of 199), NE/EP 20.0% (n = 6 of 30), NE 8.33% (n = 127 of 1525), LE 5.81% (n = 15 of 258), PA 2.33% (n = 6 of 257), and VP 1.67% (n = 10 of 600). CONCLUSIONS: The use of norepinephrine plus dobutamine was associated with lower 28-day mortality for septic shock, especially among patients with lower cardiac output.

17.
Biomed Pharmacother ; 115: 108971, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31102910

RESUMO

Sepsis, as life-threatening organ dysfunction caused by a dysregulated host response to infection, is characterized by the extensive release of cytokines and other mediators. Sini decoction (SND), a traditional Chinese prescription medicine, has been used clinically for the treatment of sepsis. But its explicit mechanism of action is still unclear. The present study aims to evaluate the potential protective effects of SND on sepsis-induced acute lung injury (ALI). After SND intervention, the lung tissues of each experimental group were collected. H&E sections were used to observe the pathological changes of lung tissue, and alveolar lavage fluid was collected to detect the infiltration of inflammatory cells. Level of inflammatory factors in lung tissue were analyzed by qRT-PCR. The change of Renin angiotensin system (RAS), as well as downstream MAPK/NF-κB signaling pathways were measured by Western blot. For in vitro experiments, human umbilical vein endothelial cells (HUVECs) were pretreated with lipopolysaccharide (LPS) and treated with SND. Subsequently, the expression levels of RAS and MAPK/NF-κB signaling pathways were measured by Western blot. In vivo, we found that SND significantly attenuated sepsis-induced pathological injury in the lung. SND also inhibited LPS-mediated inflammatory cell infiltration, the expression of pro-apoptotic proteins and the production of IL-6, IL-1ß, TNF-α and MCP-1. In vitro, experiments using a co-culture of HUVECs with SND showed that there was a decrease in pro-apoptotic protein and pro-inflammatory mediator. In this research, we also found that SND protective action could be attributed to the regulation of renin-angiotensin system (RAS). MAPKs and NF-κB pathways. To conclude, our study demonstrated that SND ameliorates sepsis-induced-ALI via regulating ACE2-Ang (1-7)-Mas axis and inhibiting the MAPK signaling pathway.

18.
Sci Rep ; 9(1): 6421, 2019 Apr 23.
Artigo em Inglês | MEDLINE | ID: mdl-31015507

RESUMO

Abnormalities of thyroid function are common in patients with nephrotic syndrome (NS). However, a limited number of studies have reported on the association between clinicopathologic features and thyroid dysfunction in patients with NS. We retrospectively studied 317 patients who had been definitively diagnosed with NS. The NS patients with thyroid dysfunction showed higher urine protein, creatinine and lipid levels and lower albumin and hemoglobin than those with normal thyroid function, with no significant differences of pathological types. After dividing thyroid dysfunction groups into five subgroups, interestingly, membranous nephropathy was the most common pathologic type, both in normal thyroid group and in subclinical hypothyroidism group (40.4% and 46.7%, respectively), followed by minimal change disease (28.1% and 21.7%, respectively); while in the hypothyroid, low T3, and low T3T4 groups minimal change disease is now the leading type (48.8%, 33.3% and 38.6%, respectively). High levels of urinary protein, creatinine, cholesterol, and platelets were independent risk factors predicting thyroid dysfunction, while higher albumin and hemoglobin were protective factors. We demonstrated that the type of renal pathology was different among NS patients in different thyroid dysfunction subgroups. Interpretation of the interactions between thyroid and renal function is a challenge for clinicians involved in the treatment of patients with NS.

19.
Chem Commun (Camb) ; 55(33): 4817-4820, 2019 Apr 18.
Artigo em Inglês | MEDLINE | ID: mdl-30946407

RESUMO

A general and selective iron-catalyzed allylic C-C(vinyl) σ-bond cleavage of allylarenes without the assistance of heteroatoms to give aryl aldehydes is reported. The unstrained carbon-carbon single bond cleavage reaction uses ambient air as the sole oxidant, proceeds efficiently at room temperature, and allows for exceptional functional-group tolerance, which addresses the long-standing challenges of current C-C bond cleavage/functionalization. Notably, the method enables rapid late-stage oxidation of complex bioactive molecules and can be used to expedite syntheses of natural products (vanillin and glucovanillin) from readily available chemical feedstocks.

20.
Nat Commun ; 10(1): 1798, 2019 04 17.
Artigo em Inglês | MEDLINE | ID: mdl-30996266

RESUMO

Biomedical research typically involves longitudinal study designs where samples from individuals are measured repeatedly over time and the goal is to identify risk factors (covariates) that are associated with an outcome value. General linear mixed effect models are the standard workhorse for statistical analysis of longitudinal data. However, analysis of longitudinal data can be complicated for reasons such as difficulties in modelling correlated outcome values, functional (time-varying) covariates, nonlinear and non-stationary effects, and model inference. We present LonGP, an additive Gaussian process regression model that is specifically designed for statistical analysis of longitudinal data, which solves these commonly faced challenges. LonGP can model time-varying random effects and non-stationary signals, incorporate multiple kernel learning, and provide interpretable results for the effects of individual covariates and their interactions. We demonstrate LonGP's performance and accuracy by analysing various simulated and real longitudinal -omics datasets.


Assuntos
Pesquisa Biomédica/métodos , Interpretação Estatística de Dados , Estudos Longitudinais , Projetos de Pesquisa , Algoritmos , Estudos de Casos e Controles , Conjuntos de Dados como Assunto , Estudos de Viabilidade , Feminino , Humanos , Modelos Lineares , Masculino , Metagenômica/métodos , Distribuição Normal , Proteômica/métodos , Software
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