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1.
Zhongguo Zhong Yao Za Zhi ; 44(21): 4768-4771, 2019 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-31872676

RESUMO

"Huangbo" is a commonly used traditional Chinese medicine in clinic,which is mainly divided into " Chuanhuangbo" and " Guanhuangbo" in the market today. It's better to use thick and dark stem bark as medicine. This study combed the germplasms of " Huangbo" in Chinese herbal medicines and ancient medical books,which showed that the name,origin and processing of " Huangbo" had undergone evolution and changes since the ancient times. The results showed that " Huangbo" was first known as " Bomu" from " Sheng Nong's Herbal Classic". There was an attached name " Tanhuan" and the alias " Huangbo" besides " Bomu" in herbal books in Tang and Song Dynasty. Before Tang Dynasty, " Bomu" was mainly originated from stems or roots of Berberis species in Berberiaceae,and then it was mainly derived from the dry bark of Phellodendron chinense in Rutaceae since Song Dynasty. In Ming Dynasty,some herbal books recorded it name as " Huangbo",whose origin and medicinal parts remained unchanged,taking Sichuan as the best producing area. During the Republic of China era,the name " Huangbo" has evolved to " Huangbo",and the variety differentiation of " Chuanhuangbo", " Guanhuangbo" and " Yanghuangbo" appeared,in which " Guanhuangbo" was mainly derived from Ph. amurense Rupr.. In modern times,although species differentiation still exists in " Huangbo",the market is still dominated by " Chuanhuangbo".


Assuntos
Medicamentos de Ervas Chinesas , Phellodendron , Livros , China , Medicina Tradicional Chinesa , Raízes de Plantas
2.
Zhongguo Zhong Yao Za Zhi ; 44(19): 4101-4106, 2019 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-31872683

RESUMO

In order to comprehensively monitor the dynamic change of Paeonia lactiflora planting area,the investigation of P. lactiflora planting area in Dangshan was carried out. It can provide reference for the planting detection of P. lactiflora in Huaibei Plain.Based on remote sensing technology,this paper extracts the planting area of P. lactiflora in Dangshan in 2018 by using the minimum distance method,maximum likelihood method,parallel hexahedron method and Mahalanobis distance method,using the remote sensing image of ZY-3 Satellite as the data source,and makes a comparative analysis with the results. The results show that the maximum likelihood method is better than the other three methods. This method can provide reference for remote sensing monitoring of P. lactiflora planting area in China.


Assuntos
Paeonia , Tecnologia de Sensoriamento Remoto , China
3.
Zhongguo Zhong Yao Za Zhi ; 44(19): 4107-4110, 2019 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-31872684

RESUMO

Moutan Cortex is one kind of famous medicinal materials. The dry root bark of Paeonia ostii which is a genuine medicinal material produced in Tongling,Anhui province,and later was introduced to Heze,Shandong province and Bozhou,Anhui province.Dangshan county is located at the northern end of Anhui province and adjacent to Shandong province. Its medicinal seedlings were came from Heze,Shandong province. At present,there is a lack of scientific investigation on the planting area of P. ostii in north China plain. On the basis of field investigation and remote sensing technology,through the data source provided by the remote sensing image of " Resources 3"( ZY-3),combined with the biological characteristics of P. ostii,the planting area of P. ostii in Dangshan county was extracted by field investigation and supervisory classification. The supervise classification method with the highest interpretation accuracy so far,the overall accuracy was 97. 81%,Kappa coefficient 0. 96. The results showed that the remote sensing classification method based on the maximum likelihood classification could extract P. ostii plots in the study area effectively. This study provides a scientific basis for the protection and rational utilization of traditional Chinese medicine resources,the development policy of traditional Chinese medicine industry and the long-term development plan in Dangshan county,and provides technical support for the poverty alleviation of traditional Chinese medicine industry in Dangshan county. It provides scientific reference for the application of remote sensing technology to investigate the planting area of P. ostii in in north China plain.


Assuntos
Medicina Tradicional Chinesa , Paeonia , Tecnologia de Sensoriamento Remoto , China
4.
Zhongguo Zhong Yao Za Zhi ; 44(15): 3261-3267, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31602881

RESUMO

To establish a DNA molecular markers method for identification of Corydalis yanhusuo,C. turtschaninovii and C. decumbens,the mat K,trn G and psb A-trn H sequences of 56 samples from 14 species of C. yanhusuo,C. turtschaninovii,C. decumbens and their related species were obtained by sequencing. The SNP loci were obtained by Bio Edit 7. 2. 2 software. The primers for AS-PCR identification were designed based on the mutation sites,and the conditions of PCR were optimized to identify C. yanhusuo,C. turtschaninovii,and C. decumbens according to the specific bands. The results showed that the amount of template( 0. 6-1 200 ng)and annealing temperature( 42-60 ℃) had little influence on the amplification results,and the number of cycles had much influence on the amplification results. When the number of cycles was 20,the specific bands of 297 bp( mat K),353 bp( trn G) and 544 bp( mat K) were amplified from C. yanhusuo,C. turtschaninovii and C. decumbens,respectively. The method established in this study had a minimum detection limit of 6 ng for C. yanhusuo,60 ng for C. decumbens and less than 0. 6 ng for C. turtschaninovii. Thus,the allelespecific PCR method established in the research can specifically identify C. yanhusuo,C. turtschaninovii,and C. decumbens.


Assuntos
Corydalis/classificação , Reação em Cadeia da Polimerase , Alelos , Corydalis/genética , Genes de Plantas , Marcadores Genéticos
5.
J Pharm Biomed Anal ; 166: 406-420, 2019 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-30711810

RESUMO

Relationships among Fallopia multiflora (Thunb.) Haraldson., F. multiflora var. angulata (S. Y. Liu) H. J. Yan, Z. J. Fang & Shi Xiao Yu., and F. multiflora var. ciliinervis (Nakai) Yonekura & H. Ohashi. were determined based on macroscopic and microscopic morphology, molecular phylogeny, and chemical analysis. The macroscopic and microscopic morphologies of root tubers or rhizomes, stems, and leaves were compared among the three taxa. The content of 11 chemical components (catechin, polydatin, stilbene glucoside, emodin, emodin-8-O-ß-D-glucopyranoside, rhein, chrysophanol, aloe-emodin, quercetin, physcion, and resveratrol) in the three taxa was determined by HPLC, and the chemical diversity was further evaluated by principal component and hierarchical cluster analyses. Molecular phylogenies were mapped using two chloroplast markers (matK and the psbA-trnH intergenic region) and a nuclear ribosomal marker [internal transcribed spacer 2 (ITS2) region]. Analyses of macroscopic and microscopic morphological characteristics revealed that the subterranean organs of F. multiflora and F. multiflora var. angulata are root tubers, whereas those of F. multiflora var. ciliinervis are rhizomes. In the phylogenetic trees, F. multiflora and F. multiflora var. angulata were clustered into a clade based on the combine matK + psbA-trnH sequence, with neighbour-joining, maximum likelihood, and Bayesian inference bootstrap support values of 99, 85, and 0.99, respectively. In addition, there were obvious differences in the chemical compositions of F. multiflora, F. multiflora var. angulata and F. multiflora var. ciliinervis. The root tubers of F. multiflora contain higher levels of stilbene glucoside and catechin, but lower levels of polydatin and anthraquinone compounds. In contrast to F. multiflora, the rhizomes of F. multiflora var. ciliinervis contain higher levels of polydatin and anthraquinone compounds, but lack stilbene glucoside. The content of all 11 assessed components was lower in F. multiflora var. angulata than in F. multiflora and F. multiflora var. cillinervis. Principal component and hierarchical cluster analyses revealed that F. multiflora and F. multiflora var. angulata individuals were clustered into a single clade, whereas F. multiflora var. ciliinervis individuals were clustered into a single clade separate from that containing F. multiflora and F. multiflora var. angulata individuals. On the basis of the results of our morphological, molecular phylogeny, and chemical analyses, we tentatively conclude that F. multiflora var. ciliinervis is an independent species, whereas F. multiflora var. angulata should be considered as a variety of F. multiflora.


Assuntos
DNA de Plantas , Medicamentos de Ervas Chinesas/análise , Fallopia multiflora/anatomia & histologia , Fallopia multiflora/química , Cromatografia Líquida de Alta Pressão , Análise por Conglomerados , Fallopia multiflora/classificação , Fallopia multiflora/genética , Limite de Detecção , Medicina Tradicional Chinesa , Microscopia de Polarização , Fotomicrografia , Filogenia , Análise de Componente Principal , Análise de Sequência de DNA , Especificidade da Espécie
6.
Zhongguo Zhong Yao Za Zhi ; 42(9): 1646-1651, 2017 May.
Artigo em Chinês | MEDLINE | ID: mdl-29082684

RESUMO

"Assessing the quality by distinguishing features of traditional Chinese medicinal materials" is a characteristic quality evaluation system of traditional Chinese medicine, and it is also the basis of "Rating according to characters and setting the price by the grade" on the market. Astragali Radix was regarded as a famous traditional Chinese medicine (TCM), and this paper has carried out herbal textual research on the development and formation of the concept, "assessing the quality by distinguishing features of traditional Chinese medicinal materials", of Astragali Radix. The authentic medicine producing areas of Astragalus in China have experienced a great change, Gansu , Sichuan and adjacent areas before the Tang Dynasty; Shanxi during the Tang and Song Dynasty. The concept, "assessing the quality by distinguishing features of traditional Chinese medicinal materials", of Astragali Radix was formed in the Song and Ming Dynasty and still used today, which described as that the shape is "straight as an arrow"; the texture is "soft as cotton"; the section looks like" gold well and jade hurdle"; it was sweet in taste and has beany flavor. The system, "assessing the quality by distinguishing features of traditional Chinese medicinal materials", of Astragali Radix has undergone the adjustments from "true or false" to "good or bad", advance with the times, pick out the advantages from others and absorb the experience of traditional identification actively. Besides, it always returns to laconism from erudition and was summarized highly. Assessing the quality by distinguishing features of traditional Chinese medicinal materials and commodity specifications have the same root, so the former has reference meaning to revise the latter.


Assuntos
Astrágalo (Planta)/química , Medicamentos de Ervas Chinesas/normas , China , Medicina Tradicional Chinesa , Farmacopeias como Assunto , Raízes de Plantas
7.
Chin Med ; 12: 14, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28533813

RESUMO

BACKGROUND: The content of medicinal bioactive constituents in huangqi is affected by plant age. In this study, we devised a quick and convenient method for determining the age of huangqi, which was cultivated in Hunyuan County (Shanxi Province). METHODS: 1, 2, 3, 4, 5, 8, 10 growth years huangqi had 38 samples, all samples were collected separately. The growth rings in these samples were observed after making paraffin section and freehand-section. The relationship between growth rings and its growth years was analyzed by SPSS 19.0 software. Histochemical localization of total flavones and saponins in huangqi was determined by color reactions. The concentration of four flavonoids and two saponins in the roots of huangqi of different ages and different organizational structure (normal roots and rotten heart roots) were determined by HPLC-DAD and HPLC-ELSD. The results were analyzed by SPSS 19.0 software. RESULTS: All huangqi samples had clear growth rings, and the statistical result about growth rings (X) and growth years (Y) showed significant correlation (r = 1, P = 0.000). The calibration curves of these six ingredients showed good linearity respectively, with significant correlation. All relative standard deviations (RSDs) of precision, recovery, repeatability, and stability experiments were less than 2%. Roots of 5-year-old plants contained the highest concentrations of total flavonoids and saponins. Saponin concentrations increased toward the center of the roots, whereas the four flavonoids showed an opposite trend in tissue distribution. CONCLUSION: The growth year of huangqi (Hunyuan County, Shanxi Province) could be determined soon and conveniently by naked eyes after staining phloroglucinol-HCl solution on freehand section. The content of saponins and flavonoids in rotten heart root and the surrounding normal tissues were affected by the formation and the extent of rotten heart.

8.
Zhongguo Zhong Yao Za Zhi ; 41(7): 1358-1360, 2016 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-28879756

RESUMO

During the fourth national survey of Chinese material medica resources inventory, 9 species of medical plants in Huangshan area of Anhui Province were newly recorded, including Microlepia calvescens, Dryopteris hangchowensis, Fatoua pilosa, Girardinia chingiana, Lecanthus peduncularis, Galium kamtschaticum, Carpesium minus,Cirsium racemiforme, Globba racemosa, which belong to seven families and nine genera. Among these, 3 genera (Girardinia, Lecanthus, Globba) are new geographical distribution in Anhui Province. All of voucher specimens are preserved in ACM. These discoveries enrich the content of flora in Anhui and provide fundamental materials for studying the plants of Anhui.


Assuntos
Plantas Medicinais/classificação , China
9.
Microsc Res Tech ; 78(5): 425-32, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-25772605

RESUMO

Authentication is the first priority when evaluating the quality of Chinese herbal medicines, particularly highly toxic medicines. The most commonly used authentication methods are morphological identification and microscopic identification. Unfortunately, these methods could not effectively evaluate some herbs with complex interior structures, such as root of Aconitum species with a circular conical shape and an interior structure with successive changes. Defining the part that should be selected as the standard plays an essential role in accurate microscopic identification. In this study, we first present a visual 3D model of Aconitum carmichaeli Debx. constructed obtained from microscopic analysis of serial sections. Based on this model, we concluded that the point of largest root diameter should be used as the standard for comparison and identification. The interior structure at this point is reproducible and its shape and appearance can easily be used to distinguish among species. We also report details of the interior structures of parts not shown in the 3D model, such as stone cells and cortical thickness. To demonstrate the usefulness of the results from the 3D model, we have distinguished the microscopic structures, at their largest segments, of the other three Aconitum species used for local habitat species of Caowu. This work provides the basis for resolution of some debate regarding the microstructural differences among these species. Thus, we conclude that the 3D model composed of serial sections has enabled the selection of a standard cross-section that will enable the accurate identification of Aconitum species in Chinese medicine.


Assuntos
Aconitum/anatomia & histologia , Aconitum/classificação , Microscopia/métodos , Imagem Tridimensional , Reprodutibilidade dos Testes
10.
Zhong Yao Cai ; 38(12): 2487-92, 2015 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-27352529

RESUMO

OBJECTIVE: To investigate the main cultivation areas of Astragalus membranaceus var. mongholicus, and to clarify its present cultivation status and commercial specification grades. METHODS: Astragalus membranaceus var. mongholicus cultivated in Hunyuan and Ying County of Shanxi, Longxi, Weiyuan and Min County of Gansu and Wuchuan, Guyang and Chifeng of Inner Mongolia were investigated, including their ecological environment, cultivation history and present situation, harvesting and processing as well as commercial specification grades. RESULTS: The cultivation methods of Astragalus membranaceus var. mongholicus changed greatly in the main cultivation areas. In Gansu and Inner Mongolia, the current major cultivation method was 2 - 3 years of seedling transplantation method, while the 3 - 5 years of seed-directly-sowed method in Inner Mongolia had been almost abandoned, and the 6 - 8 years of mountain direct seeding method was still in application in Hunyuan county of Shanxi province. On the other hand, the harvest method of Astragalus membranaceus var. mongholicus had been gradually changed from manual harvesting in ancient times to the current mechanical harvesting although the latter method did more damage to environment of cultivation areas and the resource of Astragalus membranaceus var. mongholicus in regions Hunyuan county of Shanxi. Through investigating the processing methods of Astragalus membranaceus var. mongholicus in different cultivation areas, it was found that processing method varied from area to area, and no unified specifications had been formulated on processing methods in market. In addition, the current processing method was too complicated in processing the slices of prepared Astragali Radix and most were self processing by farmers themselves, creating market disorders. Moreover, the Astragali Radix products were basically without grading classification but sold as bulk cargo. CONCLUSION: It is urgent to unify the specifications and grades of Astragali Radix product, and standardize its processing methods.


Assuntos
Agricultura/métodos , Astragalus propinquus/crescimento & desenvolvimento , Plantas Medicinais/crescimento & desenvolvimento , China , Medicamentos de Ervas Chinesas/química
11.
Zhongguo Zhong Yao Za Zhi ; 40(24): 4834-9, 2015 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-27245031

RESUMO

To understand the distribution and accumulation rules of polydatin, resveratrol, anthraglycoside B, emodin and physicion in different tissue structure of rhizome and root of Polygonum cospidatum, the content of 5 active compounds were analyzed simultaneously by HPLC, based on plant anatomy and histochemistry. The rhizome and root consist of different tissues, with an increased diameter, the proportions of the secondary xylem and phloem have increased. Resveratrol and polydatin mainly distributed in the pith, the secondary phloem and periderm of rhizome, and the secondary phloem and periderm of the root, while emodin and anthraglycoside B concentrated in the secondary structure and pith of rhizome mostly. In different thickness of the measured samples, the total contents of 5 compounds were correspondingly higher in thinner rhizome and root than those in the coarse ones.


Assuntos
Raízes de Plantas/química , Polygonum/química , Rizoma/química
12.
Zhongguo Zhong Yao Za Zhi ; 40(22): 4364-9, 2015 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-27097408

RESUMO

Puerariae Lobatae Radix, also known as Gegen, is a root derived from Pueraria lobata. Based on field investigation and the developmental anatomy of root tuber, we have elucidated the relationship between the growth of root tuber and the anomalous structure. The results of analysis showed that the root system of P. lobata was developed from seed and adventitious root and there existed root tuber, adventitious root and conductive root according to morphology and function. The root tuber was developed from adventitious root, its secondary structure conformed to the secondary structure of dicotyledon's root. With the development of root, the secondary phloem of root tuber appeared abnormal vascular tissue, which was distributed like ring in the outside of secondary vascular tissue. The root tuber might have 4-6 concentric circular permutation abnormal vascular tissuelobate, and was formed by the internal development of abnormal vascular tissue. The xylem and phloem of abnormal vascular tissue were the main body of the root tuber. The results reveal the abnormal anatomical structure development of P. lobata, also provides the theoretical basis for reasonable harvest medicinal parts and promoting sustainable utilization of resources of P. lobata.


Assuntos
Tubérculos/anatomia & histologia , Pueraria/crescimento & desenvolvimento , Raízes de Plantas/anatomia & histologia , Raízes de Plantas/crescimento & desenvolvimento , Tubérculos/crescimento & desenvolvimento , Pueraria/anatomia & histologia
13.
Zhonghua Yi Shi Za Zhi ; 44(2): 81-4, 2014 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-24989805

RESUMO

Yam was first recorded in Shen nong Ben cao Jing (Shennong's Classic of Materia Medica) which was basically came from Dioscorea opposita since ancient time. Before the Tang dynasty, it basically came from the wild species of Dioscorea opposita. In the Song Dynasty, D. opposita began to be cultivated, but Chinese medical practitioners claimed that the wild species was better than the cultivated one. After the Ming and Qing Dynasties, yam was mostly from cultivated resources. As the species quality changed, the authentic producing area of Dioscorea opposite was recognized to be in Huaiqing, Henan province, since the Ming and Qing Dynasties. Through long-term cultivation, the species produced in Huaiqing began to differentiate and the Chinese medicinal experts of successive ages considered that the product quality of yam is excellent and if it revealed the features of"white, thin, solid quality", that was the best, and this is consistent with the species called"Tiegun (iron stick)" Diascorea opposita cultivated by Huaiqing farmers. Hence, the title of"Huaiqing yam" is not the general title for the authentic species of yam produced in the Huaiqing region (now Jiaozuo city, Jiyuan city, and Xinxiang city of Henan Province), but strictly refers to that species commonly and long recognized by TCM specialists as the Tiegun Dioscorea opposita.


Assuntos
Dioscorea , Medicina Tradicional Chinesa/história , Dioscorea/crescimento & desenvolvimento , História do Século XVII , História do Século XVIII , História do Século XIX , Medicina Tradicional Chinesa/normas
14.
Zhongguo Zhong Yao Za Zhi ; 39(7): 1339-44, 2014 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-25011281

RESUMO

Fuzi is a medicine used for rescuing from collapse by restoring yang as well as a famous toxic traditional Chinese medicine. In order to ensure the efficacy and safe medication, Fuzi has mostly been applied after being processed. There have been different Fuzi processing methods recorded by doctors of previous generations. Besides, there have also been differences in Fuzi processing methods recorded in modern pharmacopeia and ancient medical books. In this study, the authors traced back to medical books between the Han Dynasty and the period of Republic of China, and summarized Fuzi processing methods collected in ancient and modern literatures. According to the results, Fuzi processing methods and using methods have changed along with the evolution of dynasties, with differences in ancient and modern processing methods. Before the Tang Dynasty, Fuzi had been mostly processed and soaked. From Tang to Ming Dynasties, Fuzi had been mostly processed, soaked and stir-fried. During the Qing Dynasty, Fuzi had been mostly soaked and boiled. In the modem times, Fuzi is mostly processed by being boiled and soaked. Before the Tang Dynasty, a whole piece of Fuzi herbs or their fragments had been applied in medicines; Whereas their fragments are primarily used in the modern times. Because different processing methods have great impacts on the toxicity of Fuzi, it is suggested to study Fuzi processing methods.


Assuntos
Química Farmacêutica/história , Química Farmacêutica/métodos , Medicamentos de Ervas Chinesas/química , Extratos Vegetais/química , China , História do Século XV , História do Século XVI , História do Século XVII , História do Século XVIII , História do Século XIX , História Antiga , História Medieval , Humanos , Medicina Tradicional Chinesa/história , Medicina Tradicional Chinesa/métodos
15.
Zhongguo Zhong Yao Za Zhi ; 38(13): 2068-72, 2013 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-24079227

RESUMO

OBJECTIVE: The microscopic structure and histochemical localization of the Polygonatum sibiricum, P. cytonema, P. filipes, P. zanlanscianense and P. odoratum was studied. METHOD: Scanning electron microscope (SEM) and digital biological microscope (DBM) were applied in the research. RESULT: Venation bundle showed in three types included collateral, amphivasal and incomplete amphivasal bundles. Morphological differences of venation bundles could be seen under the fluorescence microscope. Calcium oxalate existed in the mucilage cells looked similar to columnar crystals under SEM. In rhizome of Polygonatum, polysaccharides presented in mucilage cells, saponins and volatile oil were found in ground tissue. CONCLUSION: Microstructure difference of rhizomes would be used for identification of the Polygonatum plants. Polysaccharides and saponins are distributed in different cells of Polygonatum plants.


Assuntos
Polygonatum/química , Polygonatum/ultraestrutura , Rizoma/química , Rizoma/ultraestrutura , Oxalato de Cálcio/análise , Histocitoquímica , Microscopia Eletrônica de Varredura , Óleos Voláteis/análise , Polissacarídeos/análise , Saponinas/análise
16.
Zhongguo Zhong Yao Za Zhi ; 38(22): 3834-7, 2013 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-24558860

RESUMO

In order to guarantee the smooth progress of census work, complete the traditional Chinese medicine resources survey pilot tasks better, Anhui province founded the three-combined technical team organization model and practical management model. Around the objectives, integrating the professionality with the existing distribution of traditional Chinese medicine resources and the reality of technical team of the census in Anhui province. The technical team organization model combining universities, experts, locality, expressed the strength of all parties and formed efficient working groups. The establishment of responsible management, funds management and process management ensured that the Anhui census work had a high level of quality and relatively consistent progress. The result shows that the organization and management model of Anhui census technical team were the important guarantee of finishing the Anhui census work smoothly with great quality and quantity on time.


Assuntos
Conservação dos Recursos Naturais , Medicina Tradicional Chinesa , China , Conservação dos Recursos Naturais/economia , Conservação dos Recursos Naturais/legislação & jurisprudência , Coleta de Dados , Humanos , Medicina Tradicional Chinesa/economia , Medicina Tradicional Chinesa/normas , Projetos Piloto
17.
Microsc Res Tech ; 75(9): 1191-6, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22508220

RESUMO

Growth rings were used to determine the root age of medicinal Paeonia lactiflora from four producing areas, and their corresponding paeoniflorin content were measured based on the identification of ages. Different P. lactiflora root samples of different ages were collected from the four major growing areas in China: Bozhou, Anhui Province; Pan'an, Zhejiang Province; Zhongjiang, Sichuan Province; and Heze, Shandong Province. The relationship between the number of growth rings and age was analyzed using hand sections and paraffin sections. The paeoniflorin content in the roots of different P. lactiflora cultivars from different growing areas was measured using high-performance liquid chromatography (HPLC). The growth rings in the P. lactiflora roots were consistent with the age of the plant from Heze, Zhongjiang, Pan'an, whereas that for the P. lactiflora from Bozhou was one less than the age of the plant. The HPLC results show that the paeoniflorin content was highest in P. lactiflora 'Baihuachuanshaoyao,' followed by 'Baihuahangshaoyao,' 'Honghuachuanshaoyao,' and 'Honghuahangshaoyao,' 'Bozhoushaoyao' had the lowest levels of paeoniflorin. With increasing age, the paeoniflorin in the roots of the different P.lactiflora cultivars slowly declined or remained the same. In summary, the age of the roots of P. lactiflora from different growing areas can be determined using growth rings. The paeoniflorin content in the roots of P. lactiflora is correlated with cultivar and it was slowly declined with increasing age.


Assuntos
Benzoatos/análise , Hidrocarbonetos Aromáticos com Pontes/análise , Glucosídeos/análise , Paeonia/química , Raízes de Plantas/química , China , Cromatografia Líquida de Alta Pressão , Monoterpenos
18.
Zhonghua Yi Shi Za Zhi ; 41(6): 336-8, 2011 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-22335842

RESUMO

Shanzha is a popular Chinese herbal medicine and originally recorded in Xin Xiu Ben Cao. It was derivered form fruit of Crataegus cuneata Sieb. et Zucc form Tang dynasties until modern time. Present day, Shanzha has been recommended form fruit of Crataegus pinnatifida Bge. and C. pinnatifida Bge. var. major N.E.Br. in Chinese Pharmacopoeia. It can be seen C. cuneata has been used over 2000 years in China, so it has excellent production for using treatment. In Tang and Song dynasties, people thought Shanzha has cool nature and acid flavour, and used it in management of diagnostic criteria and principles. After Yuan dynasties, people have thought Shanzha has warm nature and acid-sweet flavour. Today, Shanzha is primarily used to remedy disease in digestive system and cardiovascular system. Through analysising Materia Medica (Ben Cao), we can find that the production and application of Shanzha is a changing process in history.

19.
Zhong Yao Cai ; 33(4): 503-6, 2010 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-20845774

RESUMO

OBJECTIVE: To appraise rationally the quality between wild and cultivated Rhizoma Atractylodis Macrocephalae. METHODS: Evaporability composition of Rhizoma Atractylodis Macrocephalae was sampled from headspace of emanating, and then response values was obtained. Principal component analysis (PCA) and discriminant factor analysis (DFA) are used to combine the optimum feature parameters, and statistical quality control analysis (SQC). RESULTS: The odor of wild Atractylodis Macrocephalae Rhizoma was diffenrent from that of the cultivated. The odor of Atractylodis Macrocephalae Rhizoma cultivated in Zhejiang was similar to the cultivated in Anhui which transplanted from Zhejiang and wild Atractylodis Macrocephalae Rhizoma which cultivated in the environment with full of sunlight. The odor of Atractylodis Macrocephalae Rhizoma cultivated in Hubei and Jiangxi were diffenrent to the odor of others. CONCLUSION: The quality of semiwild cultivated Atractylodis Macrocephalae Rhizoma was the similarest to the wild. The electronic nose combined with PCA, DFA and SQC can be applied to identify the quality of Traditional Chinese Medicine.


Assuntos
Atractylodes/química , Técnicas de Química Analítica/métodos , Medicamentos de Ervas Chinesas/análise , Eletrônica/métodos , Odorantes/análise , Algoritmos , Atractylodes/crescimento & desenvolvimento , Análise Discriminante , Medicamentos de Ervas Chinesas/química , Eletrônica/instrumentação , Plantas Medicinais/química , Plantas Medicinais/crescimento & desenvolvimento , Análise de Componente Principal , Controle de Qualidade , Rizoma/química , Sensibilidade e Especificidade
20.
Z Naturforsch C J Biosci ; 65(7-8): 495-500, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-20737920

RESUMO

Genetic relationships were studied among eight species of three taxa in the genus Chaenomeles by nuclear ribosomal internal transcribed spacer (ITS) analysis. A genetic distance matrix based on ITS sequences was estimated according to the formula of Kimura-2 parameter and a neighbour-joining phenogram, which were obtained with Clustalx4.1 software. The results showed that the germplasms of Mugua originate from Ch. speciosa (Sweet) Nakai, not including Ch. sinensis (Thouin) Kochne and Ch. cathayensis (Hemsl.) Schneid. The results also showed that 'Yao Mugua' and 'Ornamental Mugua' are the most distantly related species in germplasms.


Assuntos
DNA de Plantas/genética , Plantas Medicinais/genética , Sequência de Bases , China , DNA de Plantas/isolamento & purificação , Medicina Herbária , Medicina Tradicional Chinesa , Dados de Sequência Molecular , Preparações de Plantas/química , Reação em Cadeia da Polimerase
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