Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 10 de 10
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Eur Rev Med Pharmacol Sci ; 24(2): 837-842, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32016989

RESUMO

OBJECTIVE: To investigate the effect of single nucleotide polymorphisms (SNPs) of signal transducer and activator of transcription 3 (STAT3) on epilepsy in children. PATIENTS AND METHODS: A total of 169 children suffering from epilepsy admitted in No. 1 People's Hospital of Jining from July 2015 to December 2016 were enrolled as the research subjects. Immunohistochemistry and real time-PCR were used for analysis of the expression of STAT3 and p-STAT3 in epilepsy patients. The genotypes and alleles of rs1053005 and re744166 were analyzed through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Statistical analysis was conducted to explore the correlation between the polymorphism of STAT3 and the incidence of epilepsy in children, and the polymorphism of STAT3 in the drug-resistant and non-resistant patients was compared. RESULTS: Both the STAT3 and p-STAT3 were over-expressed in epilepsy patients. The GG genotype of rs1053005 was significantly lower in epilepsy patients than that of health control, p<0.05. By contrast, no significant difference was found in genotypes of rs744166 between epilepsy and healthy children. When comparing the genotypes of drug-resistant patients and that of non-resistant patients, the distribution of rs1053005 genotypes in the two groups showed a significant difference, p<0.05. No statistical difference was observed in rs744166 genotypes. CONCLUSIONS: STAT3 polymorphism was associated with the risk of epilepsy and drug resistance to epilepsy. This study may provide a better understanding of STAT3 in epilepsy patients and provide new targets for the treatment of epilepsy patients.

2.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 31(5): 555-559, 2019 Jul 24.
Artigo em Chinês | MEDLINE | ID: mdl-31713395

RESUMO

Exosomes are tiny vesicles secreted by most endogenous cells, and the extracellular vesicles (EVs) are specifically secreted by cells. Recently, it was found that exosomes contain a large quantity of important substances such as proteins, nucleic acids, and lipids, which play important roles in material exchange and information transmission in cell-cell communication, and in modulating the immune response, metabolism, and expansion, metastasis, and drug resistance of tumors. This paper summarizes the recent researches on exosomes in parasites and parasitic diseases and hopes to be helpful for improving the researches of parasites and parasitic diseases.


Assuntos
Exossomos , Vesículas Extracelulares , Parasitos , Doenças Parasitárias , Animais , Vesículas Extracelulares/fisiologia , Interações Hospedeiro-Parasita/fisiologia , Parasitos/citologia , Doenças Parasitárias/parasitologia , Pesquisa/tendências
3.
Genet Mol Res ; 14(4): 19225-32, 2015 Dec 29.
Artigo em Inglês | MEDLINE | ID: mdl-26782575

RESUMO

Osteoporosis is a common disease in the aging population and studies have shown that interleukin-6 (IL-6) is potentially implicated in its pathogenesis. This study was designed to assess the association between the IL-6 gene -634C/G polymorphism and osteoporosis. PubMed, Embase, China National Knowledge Infrastructure, and Wanfang databases were searched for eligible studies published up to and including December 2014 in English or Chinese. Meta-analysis was conducted by the RevMan5.2 software. Weighted mean difference and 95% confidence interval (95%CI) were calculated by a fixed-effect or random-effect model. Bone mineral density (BMD) was regarded as the assessment index. As a result, a total of four articles with 3068 subjects were included. Differences in BMD between the CC and GG genotypes were 0.03 g/cm(2) (95%CI = 0.01 to 0.05) at total body, 0.01 g/cm(2) (95%CI = 0.00 to 0.03) at femoral neck, and 0.03 g/cm(2) (95%CI = 0.00 to 0.06) at the lumbar spine (P < 0.05). For the CG versus GG genotypes, the differences in BMD were 0.03 g/cm(2) (95%CI = 0.02 to 0.05) at total body and 0.02 g/cm(2) (95%CI = 0.00 to 0.03 at the femoral neck (P < 0.05). For the CC versus CG genotypes, the differences in BMD were not significant (P > 0.05). In conclusion, the GG genotype of the -634C/G polymorphism in IL-6 appears to play a role in reducing BMD, which affects normal bone metabolism and leads to osteoporosis.


Assuntos
Densidade Óssea/genética , Interleucina-6/genética , Osteoporose/genética , Polimorfismo de Nucleotídeo Único , Regiões Promotoras Genéticas , Grupo com Ancestrais do Continente Asiático , Feminino , Colo do Fêmur/metabolismo , Colo do Fêmur/patologia , Expressão Gênica , Genótipo , Humanos , Interleucina-6/metabolismo , Vértebras Lombares/metabolismo , Vértebras Lombares/patologia , Masculino , Osteoporose/diagnóstico , Osteoporose/etnologia , Osteoporose/patologia
4.
Neoplasma ; 61(1): 63-9, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24195510

RESUMO

Cervical cancer is a multifactorial disease involving a complex interplay between genetic and environmental factors. An important role of HIF-1α in cervical cancer carcinogenesis has been studied by multiple researches. We hypothesized that there is a possible association between HIF-1α gene polymorphisms and the risk of cervical cancer in Chinese women. In a case-control study of 518 cervical cancer patients and 553 cancer-free controls, we genotyped three single-nucleotide polymorphisms (SNPs) (rs11549465, rs11549467 and rs2057482) of HIF-1α using the TaqMan SNP Genotyping Assays and assessed its associations with the cervical cancer risk. Besides, 17 cervical cancer tissues were used to assess the expression of the mature mRNA expression of HIF-1α by real-time quantitative reverse transcription PCR. We found that a significantly increased risk of cervical cancer was associated with the CC genotype of rs2057482 in the 3´-untranslated region (3'-UTR) of HIF-1α (odds ratio (OR), 1.44; 95% confidence interval (CI), 1.11-1.88), compared with the CT/TT genotypes. Moreover, the carriers of CT/TT genotypes had significantly decreased HIF-1α mRNA expression levels compared to those with CC genotype. No association was observed between the two polymorphisms (rs11549465, rs11549467) and cervical cancer risk. So that, our results provided the first insight into rs2057482 polymorphism of in the 3´-untranslated region of HIF-1α contributed to the risk of cervical cancer in a Chinese population and thus may serve as a reliable predictive factor of cervical cancer.


Assuntos
Regiões 3' não Traduzidas/genética , Predisposição Genética para Doença , Subunidade alfa do Fator 1 Induzível por Hipóxia/genética , Polimorfismo de Nucleotídeo Único , Neoplasias do Colo do Útero/genética , Adulto , Grupo com Ancestrais do Continente Asiático/genética , Estudos de Casos e Controles , Feminino , Frequência do Gene , Genótipo , Humanos , Pessoa de Meia-Idade , RNA Mensageiro/análise , Risco , Neoplasias do Colo do Útero/etiologia
5.
Neth Heart J ; 19(10): 418-22, 2011 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-21773743

RESUMO

BACKGROUND: Chinese sirolimus-eluting stents (SES) have been widely used in recent years. However, the comparison of clinical outcomes between Chinese and foreign SES remains unknown. OBJECTIVES: To compare the outcomes of Chinese SES (Firebird) with foreign SES (Cypher Select) in the treatment of patients undergoing percutaneous coronary intervention (PCI). METHODS: 4000 consecutive patients treated with SESs from January 2008 to December 2009 were included in this study. Based on the differences of the stents, the patients were divided into a Chinese SES group (Firebird; n = 2008) and a foreign SES group (Cypher Select; n = 1992). Outcomes were monitored for 1 year. The primary clinical endpoint was major adverse cardiac events (MACE): a composite of death, non-fatal myocardial infarction (MI) and target-vessel revascularisation (TVR). RESULTS: No differences were observed in the incidence of MACE (17.8% vs. 18.6%, p = 0.514) and TVR rate (9.0% vs. 8.6%, p = 0.632) during 1-year follow-up. CONCLUSIONS: Chinese SES and foreign SES have similar effects on 1-year clinical outcomes and safety.

6.
Heart ; 95(19): 1587-92, 2009 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-19592387

RESUMO

BACKGROUND: Previous studies have reported conflicting findings regarding the relation of body mass index (BMI) to outcomes following percutaneous coronary interventions (PCI). No study to date has directly examined the effect of obesity on cardiovascular thrombotic events after stent implantation. OBJECTIVE: To evaluate the effect of obesity on cardiovascular thrombotic events in patients undergoing PCI with drug-eluting stents. METHODS: We studied 4972 patients between January 2004 and December 2006. Patients were divided into three groups according to body mass index: normal (BMI <24.9 kg/m(2), n = 1284), overweight (BMI 25-29.9 kg/m(2), n = 2475) and obese (BMI > or =30 kg/m(2), n = 1213). Median follow-up was 26 (interquartile range 20-33) months. RESULTS: Composite cardiovascular thrombotic events, including cardiac death and non-fatal myocardial infarction, were significantly higher in obese patients (5.9%) than in normal (3.2%) and overweight (3.8%) patients (p = 0.001). The incidence of definite or probable stent thrombosis steadily increased with increasing body mass index (0.9% vs 1.0% vs 1.9% in normal, overweight and obese patients, respectively; p = 0.029). Multivariate analyses showed that obesity was an independent predictor of 3-year composite thrombotic events (hazard ratio 1.86; 95% confidence interval 1.25 to 2.75; p = 0.003) and definite or probable stent thrombosis (2.17; 1.04 to 4.55; p = 0.040). CONCLUSIONS: Obese patients have a higher risk for long-term cardiovascular thrombotic events following PCI with drug-eluting stents than patients with normal weight.


Assuntos
Angioplastia Coronária com Balão , Doença das Coronárias/terapia , Stents Farmacológicos , Obesidade/complicações , Trombose/etiologia , Aspirina/uso terapêutico , Prótese Vascular , Índice de Massa Corporal , Clopidogrel , Morte Súbita Cardíaca/etiologia , Feminino , Oclusão de Enxerto Vascular/etiologia , Humanos , Masculino , Pessoa de Meia-Idade , Inibidores da Agregação de Plaquetas/uso terapêutico , Estudos Retrospectivos , Ticlopidina/análogos & derivados , Ticlopidina/uso terapêutico
7.
Br J Haematol ; 126(2): 192-201, 2004 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-15238139

RESUMO

Summary The ratio of osteoprotegerin [OPG, tumour necrosis factor receptor superfamily, member 11b (TNFRSF11B)] to receptor activator of nuclear factor kappaB ligand [RANKL, tumour necrosis factor (ligand) superfamily, member 11 (TNFSF11)] in bone is critical for the regulation of bone remodelling. Myeloma cells can home to bone, triggering increased RANKL and decreased OPG expression by stromal cells, leading to osteolysis. Whether myeloma cells contribute directly to the pool of RANKL or OPG in bone has been contentious. Here we provide evidence of RANKL expression by reverse transcription polymerase chain reaction and in situ hybridization, demonstrating transcripts encoding both the membrane-bound and secreted forms of RANKL in five human multiple myeloma cell lines (LP-1, NCI-H929, OPM-2, RPMI8226, U266) and myeloma cells purified from bone marrow aspirates of myeloma patients. We demonstrated that RANKL encoding mRNAs are translated to protein by antibody detection of RANKL. In vitro assays showed that myeloma cells induced bone marrow derived mononuclear cells to differentiate into adherent tartrate-resistant acid phosphatase positive multinucleated cells, indicative of the formation of functional osteoclasts. This differentiation could also be achieved with passaged myeloma media alone, implicating secreted products. Finally, we provide evidence that the differentiation observed is at least in part the result of myeloma cell expression of RANKL. We therefore conclude that myeloma cells can directly contribute to the pool of RANKL in bone.


Assuntos
Proteínas de Transporte/genética , Leucócitos Mononucleares/patologia , Glicoproteínas de Membrana/genética , Mieloma Múltiplo/patologia , Osteoclastos/patologia , RNA Mensageiro/análise , Fosfatase Ácida/metabolismo , Diferenciação Celular , Divisão Celular , Linhagem Celular Tumoral , Meios de Cultivo Condicionados , Expressão Gênica , Humanos , Hibridização In Situ/métodos , Isoenzimas/metabolismo , Mieloma Múltiplo/metabolismo , Osteoclastos/imunologia , Isoformas de Proteínas/genética , Proteoglicanas , Ligante RANK , Receptor Ativador de Fator Nuclear kappa-B , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Sindecanas , Fosfatase Ácida Resistente a Tartarato , Células Tumorais Cultivadas
8.
Zhongguo Zhong Xi Yi Jie He Za Zhi ; 21(1): 34-6, 2001 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-12577375

RESUMO

OBJECTIVE: To observe the effect of soothing Liver and activating Spleen method (SLAS) of TCM in treating ulcerative colitis (UC). METHODS: Patients were randomly divided into 2 groups, the 33 cases in the treated group were treated with Ningxie Powder (NXP) and the 30 cases in the control group were treated with salicylazosulfapyridine (SASP). The improvement in clinical symptoms scoring, therapeutic effect in various treatment courses, changes in immune indexes as well as the condition in 1-year follow-up were observed. RESULTS: The initial therapeutic effect after 3 months treatment showed insignificant difference between the two groups. However, the effect in the treated group was better than that in the control group both short-term (after 9 months treatment) and long-term (for 1-year follow-up study) (P < 0.05). CONCLUSION: NXP, a Chinese herbal drug for soothing Liver and activating Spleen, shows good and persistent effect in treating ulcerative colitis and has favorable prospect in application.


Assuntos
Colite Ulcerativa/tratamento farmacológico , Medicamentos de Ervas Chinesas/uso terapêutico , Adolescente , Adulto , Anti-Infecciosos/uso terapêutico , Combinação de Medicamentos , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Pós , Sulfassalazina/uso terapêutico
9.
Ma Zui Xue Za Zhi ; 31(2): 117-20, 1993 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-7934683

RESUMO

Although suppression of thermoregulatory mechanisms during anaesthesia is generally assumed, the extent to which thermoregulation may be inactive is unknown. Twenty unpremedicated, ASA physical status class I patients (17 men and 3 women) scheduled for retinal detachment surgery were studied to evaluate the different changes of core and two skin-surface temperatures during halothane or isoflurane anaesthesia. Anaesthesia was induced by mask inhalation of halothane or isoflurane in nitrous oxide 70% and oxygen and was maintained by mechanical ventilation during surgery with halothane or isoflurane in nitrous oxide 50% and oxygen only. Core temperature (rectus) and skin-surface temperatures (forearm and fingertip) were measured during surgery using three separate thermometers (Y.S.I.: Yellow springs instrument Co., Inc. G541-211-Y01-33A0). Operating room temperatures were recorded in every case. Significant vasoconstriction was prospectively defined by a skin-surface temperature gradient between two sampling sites > or = 4 degrees C. The result indicated that there was no significant difference between core temperatures and skin-surface temperature gradients during halothane anaesthesia and isoflurane anaesthesia. However, three of the ten patients had their skin-surface temperature gradients > or = 4 degrees C in the halothane group. None of the ten patients had their skin-surface temperature gradients > or = 4 degrees C in the isoflurane group.


Assuntos
Anestesia por Inalação/efeitos adversos , Regulação da Temperatura Corporal/efeitos dos fármacos , Halotano/efeitos adversos , Isoflurano/efeitos adversos , Descolamento Retiniano/cirurgia , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Descolamento Retiniano/fisiopatologia
10.
Ma Zui Xue Za Zhi ; 31(1): 15-8, 1993 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-7968323

RESUMO

The purpose of this study was to determine whether prior administration of a small, sub-paralytic dose of nondepolarizing muscle relaxant, vecuronium, would have a better intubating condition than a single bolus dose when vecuronium was used as the muscle relaxant during rapid sequence induction and tracheal intubation. Six groups of 15 patients each (ASA class I or II) were involved in this study. Anesthesia was induced with fentanyl 2-3 micrograms/kg and thiopental 4-5 mg/kg. In group 1, 2 and 3, the patients were given the same priming dose of 0.1 mg/kg. Four min later, different doses of vecuronium were given to each group: 0.09 mg/kg (Gp1), 0.14 mg/kg (Gp2), and 0.19 mg/kg (Gp3). In group 4, 5 and 6, no priming vecuronium was given and the intubating doses given to each group were as follow: 0.1 mg/kg (Gp4), 0.15 mg/kg (Gp5) and 0.2 mg/kg (Gp6). At the end of a 60 sec, the percentage of patient with excellent intubating condition were 46.6%, 66.6%, 86.6%, 20%, 40% and 80% respectively for group 1, 2, 3, 4, 5 and 6 respectively. The percentage with good intubating condition were 46.6%, 33.4%, 13.4%, 46.6%, 53.2%, and 20% for group 1, 2, 3, 4, 5 and 6 respectively. The percentage of poor intubating condition were 6.8% in group 1, 33.4% in group 4, 6.8% in group 5 and 0% in group 2, 3, 6. All patients receiving a priming dose could sustain head lift for 5 sec and did not complain any respiratory discomfort 3 min after the priming dose.(ABSTRACT TRUNCATED AT 250 WORDS)


Assuntos
Intubação Intratraqueal , Brometo de Vecurônio/farmacologia , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Junção Neuromuscular/fisiologia , Transmissão Sináptica
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA