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1.
Atherosclerosis ; 297: 64-73, 2020 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-32078831

RESUMO

BACKGROUND AND AIMS: Atherosclerosis is a serious cardiovascular disease, featuring inflammation, abnormal proliferation and migration of vascular smooth muscle cells (VSMCs). During atherosclerosis, inflammation may cause low pH. T-cell death-associated gene 8 (Tdag8) is a proton-sensing receptor, however, the role of Tdag8 in VSMCs remains unknown. This study aimed to investigate the potential effects of Tdag8 in VSMCs during atherosclerosis. METHODS: We examined the expression of Tdag8 in an atherosclerotic model of high-fat-diet-fed ApoE-/- mice, while the role and mechanism of Tdag8 in phenotype transformation, proliferation and migration of VSMCs were investigated in a series of in vivo and in vitro experiments. RESULTS: We first found that Tdag8 expression at the mRNA and protein level was significantly increased in atherosclerotic ApoE-/- mice. Immunofluorescence staining showed that Tdag8 was primarily distributed in PCNA-positive VSMCs and the phenotype of VSMCs switching from contractile phenotype to synthetic phenotype. Additionally, the protein level of Tdag8 was upregulated in FBS-treated VSMCs. VSMCs proliferation and migration were inhibited by Tdag8 silencing and increased by Tdag8 overexpression. Further mechanistic studies showed that cAMP level was increased in Tdag8-overexpressing VSMCs and ApoE-/- mice. However, the PKA inhibitor H-89 reversed Tdag8-induced VSMC proliferation and migration. CONCLUSIONS: The results demonstrate that Tdag8 mediated phenotype transformation, proliferation and migration of VSMCs via the cAMP/PKA signaling pathway, thus partially contributing to atherosclerosis.

2.
Org Lett ; 21(21): 8789-8794, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31622106

RESUMO

Acyl radical triggered difunctionalizations of aryl olefins have been realized using oxime ester as the acyl precursor for the first time. Irradiation of fac-Ir(ppy)3 and oxime ester by visible light caused scission into three components, which recombined with olefins to yield significant ß-carbonyl imides showing good functional group tolerance and high atom economy. Control experiments as well as spectroscopic and electrochemical studies revealed the efficient intermolecular reorganization of oxime ester into styrene with the aid of solvent exchange.

3.
Apoptosis ; 24(7-8): 673-685, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-31177396

RESUMO

Chemotherapeutic resistance always results in poor clinical outcomes of cancer patients and its intricate mechanisms are large obstacles in overcoming drug resistance. CCR4-NOT transcription complex subunit 3 (CNOT3), a post-translational regulator, is suggested to be involved in cancer development and progression. However, its role in chemotherapeutic resistance is not well understood. In this study, after screening the CNOT3 mRNA in a cancer microarray database called Oncomine and examining the expression levels of CNOT3 mRNA in normal tissues and lung cancer tissues, we found that CNOT3 was up-regulated in lung cancer tissues. Besides, its high-expression was associated with poor prognosis of lung cancer patients. We also found higher expression level of CNOT3 and lower expression level of receptor-interacting protein kinase 3 (RIPK3) in cisplatin-resistant A549 (A549/DDP) cells, and knocking down CNOT3 expression could sensitize A549/DDP cells to cisplatin-induced apoptosis. We demonstrated that CNOT3 depletion up-regulated the expression level of RIPK3 and the enhanced apoptosis was mediated by the elevated RIPK3 to further trigger Caspase 8 activation. Taken together, our results reveal a role of CNOT3 in cisplatin resistance of lung cancer and provide a potential target for lung cancer therapy.

4.
Chemphyschem ; 20(13): 1719-1727, 2019 07 02.
Artigo em Inglês | MEDLINE | ID: mdl-31090243

RESUMO

Firefly bioluminescence is produced via luciferin enzymatic reactions in luciferase. Luciferin has to be unceasingly replenished to maintain bioluminescence. How is the luciferin reproduced after it has been exhausted? In the early 1970s, Okada proposed the hypothesis that the oxyluciferin produced by the previous bioluminescent reaction could be converted into new luciferin for the next bioluminescent reaction. To some extent, this hypothesis was evidenced by several detected intermediates. However, the detailed process and mechanism of luciferin regeneration remained largely unknown. For the first time, we investigated the entire process of luciferin regeneration in firefly bioluminescence by density functional theory calculations. This theoretical study suggests that luciferin regeneration consists of three sequential steps: the oxyluciferin produced from the last bioluminescent reaction generates 2-cyano-6-hydroxybenzothiazole (CHBT) in the luciferin regenerating enzyme (LRE) via a hydrolysis reaction; CHBT combines with L-cysteine in vivo to form L-luciferin via a condensation reaction; and L-luciferin inverts into D-luciferin in luciferase and thioesterase. The presently proposed mechanism not only supports the sporadic evidence from previous experiments but also clearly describes the complete process of luciferin regeneration. This work is of great significance for understanding the long-term flashing of fireflies without an in vitro energy supply.


Assuntos
Luciferina de Vaga-Lumes/metabolismo , Animais , Cisteína/metabolismo , Vaga-Lumes/química , Vaga-Lumes/enzimologia , Luciferina de Vaga-Lumes/química , Hidrólise , Luciferases de Vaga-Lume/metabolismo , Modelos Químicos , Estereoisomerismo , Tioléster Hidrolases/metabolismo
5.
Environ Pollut ; 250: 233-240, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30999200

RESUMO

Microbes in sediments contribute to nutrient release and play an important role in lake eutrophication. However, information about the profiles of functional genes and bacterial communities and the most important environmental factor affecting them in the sediments of eutrophic lake remains unrevealed. In this work, the real-time fluorescent quantitative polymerase chain reaction (qPCR) assay and 16S ribosomal RNA gene next generation sequencing analysis were used to explore the profiles of functional genes and bacterial communities in the sediments of Chaohu Lake. The selected 18 functional genes involved in C, N and P cycles were detected in most of samples. Seasonal variation and sediment variables were found to affect the profiles of functional genes and bacterial communities, and total nitrogen was the dominant environmental factor to drive the formation of bacterial community structure. Proteobacteria and Firmicutes were observed to be the two dominant phyla in the sediments with relative abundance ranging from 10.8% to 36.0% and 7.7%-46.7%, respectively. Three bacterial phyla, i.e., Actinobacteria, Proteobacteria, and Spirochaetes, were found to be significantly positively correlated with the C, N and P-cycle related functional genes. Bacterial community structure was the most important driver to shape the profiles of functional genes. Seasonal variation also influenced the co-occurrence patterns between functional genes and bacterial taxa as revealed by network analysis. The findings from this work facilitate a better understanding about the C, N, and P cycles in the sediments of eutrophic lakes.


Assuntos
Sedimentos Geológicos/microbiologia , Lagos/microbiologia , Microbiota/genética , Nitrogênio/química , Estações do Ano , Bactérias/classificação , Bactérias/genética , Bactérias/isolamento & purificação , Bactérias/metabolismo , Proteínas de Bactérias/genética , Eutrofização , Sedimentos Geológicos/química , Lagos/química , Nitrogênio/metabolismo , RNA Ribossômico 16S/genética , Microbiologia da Água
6.
Mol Immunol ; 105: 76-85, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30496979

RESUMO

Activation of NLRP3 inflammasomes is crucial in the pathological process of Ulcerative colitis (UC), which could be negatively regulated by PINK1/Parkin-driven mitophagy. Palmatine is a herb derived isoquinoline alkaloid with potent anti-inflammatory and anti-bacteria activities. In present study, we evaluated the effect of palmatine on dextran sulfate sodium (DSS)-induced mice colitis and examined whether its effect is exerted by promoting mitophagy-mediated NLRP3 inflammasome inactivation. The result showed that palmatine (40, 100 mg/kg) significantly prevented bodyweight loss and colonic shortening in DSS mice, and reduced the disease activity index and histopathologic score. The levels of MPO, IL-1ß, TNF-α and the number of F4/80+ cells in colon of DSS mice were remarkably decreased by palmatine. Moreover, palmatine suppressed NLRP3 inflammasomes activation, but enhanced the expression of the mitophagy-related proteins involving LC3, PINK1 and Parkin in colonic tissue of DSS mice. These effects was consistent with the in vitro data revealing that palmatine inhibited the activation of NLRP3 inflammasomes, while promoted the expression and mitochondrial recruitment of PINK1 and Parkin in THP-1 cell differentiated macrophages. Furthermore, the effect of palmatine on THP-1 cells was neutralized by a mitophagy inhibitor Cyclosporin A (CsA) and PINK1-siRNA. In parallel, CsA significantly attenuated the therapeutic effect of palmatine in DSS mice, illustrating that the anti-colitis effect of palmatine is closely related to mitophagy. Taken together, the current results demonstrated that palmatine protected mice against DSS-induced colitis by facilitating PINK1/Parkin-driven mitophagy and thus inactivating NLRP3 inflammasomes in macrophage.


Assuntos
Colite , Sulfato de Dextrana/toxicidade , Inflamassomos/imunologia , Proteína 3 que Contém Domínio de Pirina da Família NLR/imunologia , Ácido Palmítico/farmacologia , Animais , Colite/induzido quimicamente , Colite/imunologia , Colite/patologia , Colite/prevenção & controle , Ciclosporina/farmacologia , Modelos Animais de Doenças , Humanos , Masculino , Camundongos Endogâmicos BALB C , Proteínas Quinases/imunologia , Células THP-1 , Ubiquitina-Proteína Ligases/imunologia
7.
Appl Microbiol Biotechnol ; 102(24): 10791-10801, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30334090

RESUMO

Iron reduction mediated by Fe(III)-reducing bacteria (FeRB) occurs in aqueous environments and plays an essential role in removing contaminates in polluted freshwater lakes. Two model FeRB species, Shewanella and Geobacter, have been intensively studied because of their functions in bioremediation, iron reduction, and bioelectricity production. However, the abundance and community diversity of Shewanella and Geobacter in eutrophic freshwater lakes remain largely unknown. In this work, the distribution, abundance and biodiversity of Shewanella, Geobacter and other FeRB in the sediments of a heavily polluted lake, Chaohu Lake, China, across four successive seasons were investigated. Shewanella, Geobacter, and other FeRB were found to be widely distributed in the sediment of this heavily eutrophic lake. Geobacter was abundant with at least one order of magnitude more than Shewanella in cold seasons. Three Shewanella-related operational taxonomic units were detected and sixty one Geobacter-related operational taxonomic units were grouped into three phylogenetic clades. Thiobacillus, Desulfuromonas and Geobacter were identified as the main members of FeRB in the lake sediments. Interestingly, nutrients like carbon, nitrogen, and phosphorus were found to be the key factors governing the abundance and diversity of FeRB. Total FeRB, as well as Geobacter and Shewanella, were more abundant in the heavily eutrophic zone than those in the lightly eutrophic zone. The abundance and diversity of FeRB in the sediments of freshwater lakes were highly related with the degree of eutrophication, which imply that FeRB might have a great potential in alleviating the eutrophication and contamination in aqueous environments.


Assuntos
Geobacter , Sedimentos Geológicos/microbiologia , Ferro/metabolismo , Lagos/microbiologia , Shewanella , Bactérias/genética , Bactérias/isolamento & purificação , Biodiversidade , China , Clonagem Molecular , Eutrofização , Geobacter/genética , Geobacter/isolamento & purificação , Sedimentos Geológicos/química , Sequenciamento de Nucleotídeos em Larga Escala , Oxirredução , Filogenia , Shewanella/genética , Shewanella/isolamento & purificação , Microbiologia da Água , Poluição da Água
8.
J Am Heart Assoc ; 7(19): e009216, 2018 10 02.
Artigo em Inglês | MEDLINE | ID: mdl-30371325

RESUMO

Background Prostaglandin E2 has long been known to be an immune modulator. It is released after tissue injury and plays a role in modulating macrophage activities, which are essential for tissue regeneration. However, the involvement of prostaglandin E2 receptor 2 ( EP 2)-dependent regulation of macrophages in postischemic heart is unclear. This study aims to evaluate the role of EP 2 in damaged heart. Methods and Results The effect of EP 2 in postischemic heart was evaluated using EP 2-deficient transgenic mice. We demonstrated that cardiac function was worse after myocardial injury on loss of EP 2. Furthermore, EP 2 deficiency also altered proinflammatory response and resulted in a defect in macrophage recruitment to the injured myocardium. Transcriptome analysis revealed that the expression of erythroid differentiation regulator 1 ( Erdr1) was significantly induced in EP 2-deficient macrophages. Knocking down Erdr1 expression restored migration ability of EP 2-deficient cells both in vitro and in vivo. By using a genetic fate-mapping approach, we showed that abolishment of EP 2 expression effectively attenuated cell replenishment. Conclusions The EP 2-dependent signaling pathway plays a critical role in regulating macrophage recruitment to the injured myocardium, thereby exerting a function in modulating the inflammatory microenvironment for cardiac repair.


Assuntos
Ativação de Macrófagos/fisiologia , Macrófagos/metabolismo , Infarto do Miocárdio/metabolismo , Miocárdio/metabolismo , Receptores de Prostaglandina E Subtipo EP2/metabolismo , Animais , Células Cultivadas , Modelos Animais de Doenças , Feminino , Citometria de Fluxo , Macrófagos/patologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Infarto do Miocárdio/patologia , Miocárdio/patologia , Transdução de Sinais
9.
Huan Jing Ke Xue ; 39(10): 4512-4518, 2018 Oct 08.
Artigo em Chinês | MEDLINE | ID: mdl-30229598

RESUMO

Physiological responses of ryegrass in cadmium-nonylphenol (Cd-NP) co-contaminated water, as well as the phytoremediation effect were studied under laboratory conditions. The experiment revealed that the biomass and chlorophyll content of ryegrass significantly declined at high concentrations of Cd2+(10 mg·L-1), while POD and PPO activities significantly increased. No significant changes to the biomass, chlorophyll content and MDA of ryegrass were observed in the presence of NP. POD activities significantly increased at high concentrations of NP (5 mg·L-1). The addition of high NP concentrate reduced the inhibition of Cd under co-contaminated stress, the MDA content and PPO activities both reduced. The experiment also revealed that the removal rate of Cd2+ by ryegrass reached 55.3% after 12 h. The absorption efficiency was improved by high NP concentration. Absorption efficiency of Cd2+ by the subterranean part of the plant significantly increased in presence of NP, and the absorption efficiency increased with the increase of NP concentration. The absorption rate of NP was 44.6% after 24 h at NP concentration of 5 mg·L-1. The removal rate was slightly influenced at low concentrations of Cd2+, but significant inhibition of absorption and degradation was observed at high concentrations of Cd2+.


Assuntos
Biodegradação Ambiental , Cádmio/análise , Lolium/fisiologia , Fenóis/análise , Poluentes Químicos da Água/análise , Raízes de Plantas
10.
Chemistry ; 24(37): 9340-9352, 2018 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-29710377

RESUMO

The luciferin/luciferase system of the firefly has been used in bioluminescent imaging to monitor biological processes. In order to enhance the efficiency and expand the application range, some efforts have been made to tune the light emission, especially the effort to obtain NIR light. However, those case-by-case studies have not together revealed the nature and mechanism of the color tuning. In this paper, we theoretically investigated the fluorescence of all kinds of typical oxyluciferin analogues. The present systematical modifications of both oxyluciferin and luciferase indicate that the essential factor affecting the emission color is the charge distribution (or the electric dipole moment) on the oxyluciferin, which impacts on the charge transfer to form the light emitter and, subsequently, influence the strength and wavelength of the emission light. More negative charge distributed on the "thiazolone moiety" of the oxyluciferin or its analogues leads to a redshift. Based on this conclusion, we theoretically designed optimal pairs of luciferin analogue and luciferase for emitting NIR light, which could inspire new synthetic procedures and practical applications.


Assuntos
Corantes Fluorescentes/química , Indóis/química , Medições Luminescentes/métodos , Modelos Moleculares , Pirazinas/química , Espectroscopia de Luz Próxima ao Infravermelho/métodos , Animais , Luciferina de Vaga-Lumes/química , Fluorescência , Expressão Gênica , Luz , Luciferases de Vaga-Lume/química , Luciferases de Vaga-Lume/genética , Mutação
11.
Sci Total Environ ; 637-638: 926-933, 2018 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-29763873

RESUMO

Shewanella species have a diverse respiratory ability and wide distribution in environments and play an important role in bioremediation and the biogeochemical cycles of elements. Primers with more accuracy and broader coverage are required with consideration of the increasing number of Shewanella species and evaluation of their roles in various environments. In this work, a new primer set of 640F/815R was developed to quantify the abundance of Shewanella species in natural and engineered environments. In silico tools for primer evaluation, quantitative polymerase chain reaction (qPCR) and clone library results showed that 640F/815R had a higher specificity and coverage than the previous primers in quantitative analysis of Shewanella. Another newly developed primer pair of 211F/815cR was also adopted to analyze the Shewanella diversity and demonstrated to be the best candidate in terms of specificity and coverage. We detected more Shewanella-related species in freshwater environments and found them to be substantially different from those in marine environments. Abundance and diversity of Shewanella species in wastewater treatment plants were largely affected by the process and operating conditions. Overall, this study suggests that investigations of abundance and diversity of Shewanella in various environments are of great importance to evaluate their ecophysiology and potential ecological roles.


Assuntos
Monitoramento Ambiental/métodos , Shewanella/crescimento & desenvolvimento , Águas Residuárias/microbiologia , Microbiologia da Água , Biodegradação Ambiental , Primers do DNA , Filogenia , Reação em Cadeia da Polimerase , Shewanella/classificação
12.
Chemosphere ; 187: 62-69, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-28841432

RESUMO

Although microbial synthesized palladium nanoparticles (bioPd) have been demonstrated to exhibit a great activity toward dechlorination of several chlorinated pollutants, there is no systematic investigation into the substituent effect on dechlorination. Chloronitrobenzenes are widely used for manufacturing and known as persistent pollutants with recalcitrance of biodegradation for nitro groups. In this work, bioPd was synthesized by Shewanella oneidensis MR-1. The dechlorination of 2-chloronitrobenzene, 4-chloronitrobenzene and 2,4-dichloronitrobenzene catalyzed by bioPd were investigated. Simultaneous dechlorination and nitro reduction were observed by synergistic catalysis of bioPd and S. oneidensis MR-1. Pd concentration was optimized for the reduction. Producing profiles of intermediates changed with the ratio of Pd to cell, supporting a size- or shape-controlled catalytic activity of bioPd. The removal of chloro atoms at para-position was easier than that at ortho-position in 2,4-DCNB, suggesting a position effect on the reduction, which was further supported by the frontier molecular orbital and frontier electron density of 2,4-DCNB according to density functional theory.


Assuntos
Biodegradação Ambiental , Halogenação , Paládio/metabolismo , Shewanella/metabolismo , Catálise , Nanopartículas/química , Nitrobenzenos/metabolismo
13.
Arch Immunol Ther Exp (Warsz) ; 65(2): 145-156, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-27480649

RESUMO

Caffeic acid derivatives constitute a class of potent anti-inflammatory and cardioprotective drug candidates. We recently synthesized a new caffeic acid derivative N-propyl caffeamide (PCA). Our pilot experiments demonstrated that PCA enhanced the survival of rat cardiomyocyte H9c2 cells against oxygen glucose deprivation and reoxygenation challenge in a concentration-dependent manner. Interestingly, PCA exhibited better cardioprotective potential than caffeic acid phenethyl ester and propyl caffeate. Thus, we hypothesized that PCA could protect heart against ischemia reperfusion (I/R) injury in mice. We first determined the stability and pharmacokinetic profile of PCA in male Sprague-Dawley rats by ultra-performance liquid chromatography coupled with UV and MS/MS detections. The stability of PCA in rat plasma was defined by the half-life of 31.39, 7.19 and 1.37 h in rat plasma at 25, 37 and 60 °C, respectively. To study the pharmacokinetic profiles, PCA was injected into male SD rats at the dose of 15 mg/kg via intravenous bolus administration. PCA showed the elimination half-life of approximate 235 min in rats. We subsequently evaluated the cardioprotective potential of PCA in mice model of myocardial infarction. Our results demonstrated that PCA effectively reduced infarct size and release of myocardial enzymes (e.g., CK, CK-MB and LDH). Biochemical analyses suggested that PCA increased the activities of antioxidant enzymes (e.g., CAT and SOD) while attenuated lipid peroxidation. Moreover, PCA profoundly reduced the number of apoptotic cells in infarcted myocardium. Consistently, PCA increased the expression level of anti-apoptotic protein Bcl2 whereas suppressed the expression of pro-apoptotic protein Bax in cardiac tissues. Collectively, PCA appears to be a novel bioavailable and stable pharmacological treatment for myocardial infarction.


Assuntos
Ácidos Cafeicos/química , Miócitos Cardíacos/efeitos dos fármacos , Traumatismo por Reperfusão/prevenção & controle , Animais , Antioxidantes/metabolismo , Apoptose , Biomarcadores/metabolismo , Ácidos Cafeicos/farmacocinética , Sobrevivência Celular/efeitos dos fármacos , Cromatografia Líquida de Alta Pressão , Relação Dose-Resposta a Droga , Glucose/química , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Estresse Oxidativo , Oxigênio/química , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Ratos , Ratos Sprague-Dawley , Traumatismo por Reperfusão/metabolismo , Espectrofotometria Ultravioleta , Espectrometria de Massas em Tandem , Proteína X Associada a bcl-2/metabolismo
14.
Zhongguo Zhong Xi Yi Jie He Za Zhi ; 37(2): 225-231, 2017 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-30650278

RESUMO

Objective To observe the effects of Xinfeng Capsule (XFC) at different doses on lung function, Thl/Th2 cells, regulatory T cells (Treg) in adjuvant arthritis (AA) rats. Methods Totally 84 rats were randomly divided into 5 groups, i.e., the normal control group (NC) , the model group (M) , the methotrexate (MTX) group, the Tripterygium Glycosides Table (TGT) group, the low dose XFC (XFC- L) group, the medium dose XFC (XFC-M) group, the high dose XFC (XFC-H) group, 12 in each group. Freund's complete adjuvant (FCA; 0. 1 mL) was intradermally injected to all rats except those in the NC group from right rear paw to induce inflammation. Medication was started from the 19th day after inflam- mation. Normal saline was administered to rats in the NC group and the M group. Rats in the rest groups were correspondingly administered with MTX, TGT, XFC, respectively. Changes of each index were ob- served in all groups. Results (1) Compared with the NC group, rat paw swelling degree (E) , arthritis index (AI) , lung index (LI) , average expiratory flow in 1 second (FEV1/FVC%) , alveolitis integral, TNF- α, Th1/Th2 cells, transforming growth factor-ß1 ( TGF-ß1 ) expression significantly increased in the M group (P <0. 01) ; forced vital capacity (FVC) , peak expiratory flow 25% of vital capacity (FEF25), peak expiratory flow 50% of vital capacity (FEF50), peak expiratory flow 75% of vital capacity (FEF75), the maximum mid-expiratory flow (MMF) , peak expiratory flow (PEF) , CD4 ⁺Treg, CD4⁺CD25 ⁺Treg, IL-10, and Foxp3 expression significantly decreased in the M group (P <0. 01). (2) Compared with the M group, body weight, FVC, FEF25, FEF50, FEF75, MMF, PEF, IL-10, Treg, and Foxp3 expression increased in all treatment groups; E, Al, LI, FEV1/FVC%, TNF-α, Th1/Th2 cells, and TGF-ß1 expression decreased in all treatment groups (P <0. 05, P <0. 01). (3) Compared with the XFC-M group, LI, alveolitis integral, TNF- α, Th1/Th2 cells, and TGF-ß1 increased; FVC, FEF25, FEF50, FEF75, IL-10, CD4⁺Treg, CD4⁺CD25⁺ Treg, and Foxp3 decreased in other treatment groups (P <0. 05, P <0. 01). Conclusions AA rats had local swollen paws and decreased lung function. XFC could significantly improve paw swelling and Al of AA rats, and improve lung function. It could reduce inflammatory reaction and immune complexes on tis- sue and organ damage, improve joint and pulmonary symptoms possibly through promoting expressions of IL-10, CD4⁺Treg, CD4⁺CD25⁺Treg, and Foxp3, and inhibiting TNF-α,Th1/Th2 cells, and TGF-ß1 ex- pression.


Assuntos
Artrite Experimental , Medicamentos de Ervas Chinesas , Linfócitos T Reguladores , Animais , Artrite Experimental/tratamento farmacológico , Artrite Experimental/metabolismo , Medicamentos de Ervas Chinesas/farmacologia , Pulmão/fisiologia , Ratos , Testes de Função Respiratória , Células Th1 , Células Th2
15.
Huan Jing Ke Xue ; 38(1): 294-300, 2017 Jan 08.
Artigo em Chinês | MEDLINE | ID: mdl-29965059

RESUMO

The influence of Cd on the degradation of nonylphenol (NP) by P.aeruginosa SH1 was investigated in this study.The investigation revealed that biomass of the strain was significantly declined with the increase of Cd2+ concentration.The biomass was declined by 27.1% in the presence of 10 mg·L-1Cd2+ after 24 h.The addition of Cd2+ had a great influence on adsorption of NP by the strain.As for the effect of living stain,adsorption by P.aeruginosa SH1 cells was stimulated at low concentrations of Cd2+(0.5 mg·L-1),while inhibited at higher levels (≥5 mg·L-1).As for inactivation of microbes,adsorption by cells was stimulated at higher concentrations,but was only slightly influenced at low levels.The results showed that the intracellular enzymes had much greater degradation rate than the living cells.Different concentrations of Cd2+ had different effects on bacteria and intracellular enzyme degradation of NP separately.The degradation efficiency when using intracellular enzymes and bacteria was inhibited at higher levels of Cd2+ and the intracellular enzyme inhibition was more significant.Degradation by cells was stimulated at low concentrations of Cd2+,but no significant impact was found on that by intracellular enzymes.The degradation process of NP by intracellular enzymes of the strain conformed to the first-order kinetic model.The highest reaction rate was achieved when the concentrations of Cd2+ was 0.5 mg·L-1 and the half-life of this substrate was 5.5 h.However,the degradation process of NP by the strain did not conform to the first-order kinetic model.


Assuntos
Biodegradação Ambiental , Cádmio/química , Fenóis/metabolismo , Pseudomonas aeruginosa/metabolismo
16.
EMBO Mol Med ; 9(2): 251-264, 2017 02.
Artigo em Inglês | MEDLINE | ID: mdl-28011860

RESUMO

Although remnant cardiomyocytes (CMs) possess a certain degree of proliferative ability, efficiency is too low for cardiac regeneration after injury. In this study, we identified a distinct stage within the initiation phase of CM reprogramming before the MET process, and microarray analysis revealed the strong up-regulation of several mitosis-related genes at this stage of reprogramming. Several candidate genes were selected and tested for their ability to induce CM proliferation. Delivering a cocktail of three genes, FoxM1, Id1, and Jnk3-shRNA (FIJs), induced CMs to re-enter the cell cycle and complete mitosis and cytokinesis in vitro More importantly, this gene cocktail increased CM proliferation in vivo and significantly improved cardiac function and reduced fibrosis after myocardial infarction. Collectively, our findings present a cocktail FIJs that may be useful in cardiac regeneration and also provide a practical strategy for probing reprogramming assays for regeneration of other tissues.


Assuntos
Proliferação de Células , Terapia Genética/métodos , Infarto do Miocárdio/terapia , Miócitos Cardíacos/fisiologia , Regeneração , Animais , Dependovirus/genética , Proteína Forkhead Box M1/genética , Vetores Genéticos , Proteína 1 Inibidora de Diferenciação/genética , Camundongos Endogâmicos C57BL , Proteína Quinase 10 Ativada por Mitógeno/genética , RNA Interferente Pequeno/genética , Medicina Regenerativa/métodos , Transdução Genética , Resultado do Tratamento
17.
Cell Mol Life Sci ; 74(10): 1805-1817, 2017 05.
Artigo em Inglês | MEDLINE | ID: mdl-27999898

RESUMO

Mouse embryonic stem cells (mESCs), characterized by their pluripotency and capacity for self-renewal, are driven by a complex gene expression program composed of several regulatory mechanisms. These mechanisms collaborate to maintain the delicate balance of pluripotency gene expression and their disruption leads to loss of pluripotency. In this review, we provide an extensive overview of the key pillars of mESC pluripotency by elaborating on the various essential transcription factor networks and signaling pathways that directly or indirectly support this state. Furthermore, we consider the latest developments in the role of epigenetic regulation, such as noncoding RNA signaling or histone modifications.


Assuntos
Epigênese Genética , Regulação da Expressão Gênica no Desenvolvimento , Células-Tronco Embrionárias Murinas/metabolismo , Ativação Transcricional , Animais , Diferenciação Celular , Proliferação de Células , Montagem e Desmontagem da Cromatina , Redes Reguladoras de Genes , Código das Histonas , Camundongos , Células-Tronco Embrionárias Murinas/citologia , RNA não Traduzido/genética , RNA não Traduzido/metabolismo , Transdução de Sinais , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo
18.
Appl Environ Microbiol ; 83(4)2017 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-27986717

RESUMO

Manipulation of biofilm formation in Shewanella is beneficial for application to industrial and environmental biotechnology. BpfA is an adhesin largely responsible for biofilm formation in many Shewanella species. However, the mechanism underlying BpfA production and the resulting biofilm remains vaguely understood. We previously described the finding that BpfA expression is enhanced by DosD, an oxygen-stimulated diguanylate cyclase, under aerobic growth. In the present work, we identify FlrA as a critical transcription regulator of the bpfA operon in Shewanella putrefaciens CN32 by transposon mutagenesis. FlrA acted as a repressor of the operon promoter by binding to two boxes overlapping the -10 and -35 sites recognized by σ70 DosD regulation of the expression of the bpfA operon was mediated by FlrA, and cyclic diguanylic acid (c-di-GMP) abolished FlrA binding to the operon promoter. We also demonstrate that FlhG, an accessory protein for flagellum synthesis, antagonized FlrA repression of the expression of the bpfA operon. Collectively, this work demonstrates that FlrA acts as a central mediator in the signaling pathway from c-di-GMP to BpfA-associated biofilm formation in S. putrefaciens CN32. IMPORTANCE: Motility and biofilm are mutually exclusive lifestyles, shifts between which are under the strict regulation of bacteria attempting to adapt to the fluctuation of diverse environmental conditions. The FlrA protein in many bacteria is known to control motility as a master regulator of flagellum synthesis. This work elucidates its effect on biofilm formation by controlling the expression of the adhesin BpfA in S. putrefaciens CN32 in response to c-di-GMP. Therefore, FlrA plays a dual role in controlling motility and biofilm formation in S. putrefaciens CN32. The cooccurrence of flrA, bpfA, and the FlrA box in the promoter region of the bpfA operon in diverse Shewanella strains suggests that bpfA is a common mechanism that controls biofilm formation in this bacterial species.


Assuntos
Adesinas Bacterianas/genética , Biofilmes/crescimento & desenvolvimento , Proteínas Repressoras/genética , Shewanella putrefaciens/metabolismo , Transcrição Genética/genética , Adesinas Bacterianas/metabolismo , Proteínas de Bactérias/genética , Sequência de Bases , GMP Cíclico/análogos & derivados , GMP Cíclico/farmacologia , Flagelos/metabolismo , Regulação Bacteriana da Expressão Gênica/genética , Regiões Promotoras Genéticas/genética , Shewanella putrefaciens/genética
19.
Sci Rep ; 6: 39098, 2016 12 19.
Artigo em Inglês | MEDLINE | ID: mdl-27991531

RESUMO

Although the capacity for electroactive bacteria to convert environmental metallic minerals and organic pollutants is well known, the role of the redox properties of microbial extracellular polymeric substances (EPS) in this process is poorly understood. In this work, the redox properties of EPS from two widely present electroactive bacterial strains (Shewanella oneidensis and Pseudomonas putida) were explored. Electrochemical analysis demonstrates that the EPS extracted from the two strains exhibited redox properties. Spectroelectrochemical and protein electrophoresis analyses indicate that the extracted EPS from S. oneidensis and P. putida contained heme-binding proteins, which were identified as the possible redox components in the EPS. The results of heme-mediated behavior of EPS may provide an insight into the important roles of EPS in electroactive bacteria to maximize their redox capability for biogeochemical cycling, environmental bioremediation and wastewater treatment.


Assuntos
Espaço Extracelular/metabolismo , Polímeros/química , Pseudomonas putida/metabolismo , Shewanella/metabolismo , Proteínas de Bactérias/química , Proteínas de Bactérias/metabolismo , Biodegradação Ambiental , Proteínas de Transporte/química , Proteínas de Transporte/metabolismo , Técnicas Eletroquímicas , Hemeproteínas/química , Hemeproteínas/metabolismo , Oxirredução , Polímeros/metabolismo , Pseudomonas putida/química , Shewanella/química
20.
Photochem Photobiol ; 92(4): 552-60, 2016 07.
Artigo em Inglês | MEDLINE | ID: mdl-27165852

RESUMO

Firefly bioluminescence has been applied in several fields. However, the absorption and fluorescence spectra of the substrate, luciferin, have not been observed at the vibrational level. In this study, the vibrationally resolved absorption and fluorescence spectra of firefly luciferin (neutral form LH2 , phenolate ion form LH(-) and dianion form L(2-) ) are simulated using the density functional method and convoluted by a Gaussian function, with displacement, distortion and Duschinsky effects in the framework of the Franck-Condon approximation. Both neutral and anionic forms of the luciferin are considered in the gas phase and in solution. The simulated spectra have desired band maxima with the experimental ones. The vibronic structure analysis reveals that the features of the most contributive vibrational modes coincide with the key geometry-changing region during transition between the ground state and the first singlet excited state.


Assuntos
Simulação por Computador , Luciferina de Vaga-Lumes/química , Espectrometria de Fluorescência , Espectrofotometria Atômica , Gases , Modelos Químicos , Estrutura Molecular , Psicoterapia Breve
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