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1.
Genome Biol ; 19(1): 144, 2018 09 25.
Artigo em Inglês | MEDLINE | ID: mdl-30253806

RESUMO

BACKGROUND: Histone lysine acylations by short-chain fatty acids are distinct from the widely studied histone lysine acetylation in chromatin, although both modifications are regulated by primary metabolism in mammalian cells. It remains unknown whether and how histone acylation and acetylation interact to regulate gene expression in plants that have distinct regulatory pathways of primary metabolism. RESULTS: We identify 4 lysine butyrylation (Kbu) sites (H3K14, H4K12, H2BK42, and H2BK134) and 45 crotonylation (Kcr) sites on rice histones by mass spectrometry. Comparative analysis of genome-wide Kbu and Kcr and H3K9ac in combination with RNA sequencing reveals 25,306 genes marked by Kbu and Kcr in rice and more than 95% of H3K9ac-marked genes are marked by both. Kbu and Kcr are enriched at the 5' region of expressed genes. In rice under starvation and submergence, Kbu and Kcr appear to be less dynamic and display changes in different sets of genes compared to H3K9ac. Furthermore, Kbu seems to preferentially poise gene activation by external stresses, rather than internal circadian rhythm which has been shown to be tightly associated with H3K9ac. In addition, we show that rice sirtuin histone deacetylase (SRT2) is involved in the removal of Kcr. CONCLUSION: Kbu, Kcr, and H3K9ac redundantly mark a large number of active genes but display different responses to external and internal signals. Thus, the proportion of rice histone lysine acetylation and acylation is dynamically regulated by environmental and metabolic cues, which may represent an epigenetic mechanism to fine-tune gene expression for plant adaptation.


Assuntos
Histonas/metabolismo , Lisina/metabolismo , Oryza/metabolismo , Processamento de Proteína Pós-Traducional , Acetilação , Regulação da Expressão Gênica de Plantas , Histonas/química , Oryza/genética , Proteínas de Plantas/metabolismo , Sirtuínas/metabolismo , Estresse Fisiológico/genética
2.
Int J Low Extrem Wounds ; 16(3): 154-162, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28741388

RESUMO

Traditional Chinese medicine has great potential to improve wound healing. ANBP, the mixture of 4 Chinese herbs- Agrimoniapilosa, Nelumbonucifera, Boswelliacarteri, and Pollen typhae-is effective in trauma treatment while its mechanism is still elusive. In this study, quantitative proteomics and bioinformatics analyses were performed to decipher the possible roles of ANBP in accelerated wound healing of mouse skin. Among all 3171 identified proteins, 90, 71, 80, and 140 proteins were found to be differently expressed in 6 hours, 3 days, 7 days, and 14 days ANBP-treated tissues compared with corresponding control tissues, respectively. The result showed that different biological processes and pathways were activated at different healing stages. At the early healing stage, ANBP treatment mainly affected several biological processes, including immune and defense response, vascular system restoration, hemostasis and coagulation regulation, lipid metabolism and signal transduction, while muscle tissue, hair, epidermis, extracellular matrix and tissue remodeling related activities were the major events in ANBP promoted later wound healing. This is the first quantitative proteome study of ANBP-treated wound tissues, which provide a new perspective for the mechanism of ANBP accelerated wound healing and is of guiding significance for clinical application of ANBP in trauma disorders cure.


Assuntos
Medicamentos de Ervas Chinesas/uso terapêutico , Proteômica , Cicatrização/efeitos dos fármacos , Ferimentos e Lesões/tratamento farmacológico , Ferimentos e Lesões/patologia , Animais , Biópsia por Agulha , Modelos Animais de Doenças , Imuno-Histoquímica , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Distribuição Aleatória , Valores de Referência , Sensibilidade e Especificidade , Pele/efeitos dos fármacos , Pele/patologia , Cicatrização/genética , Ferimentos e Lesões/genética
3.
Yi Chuan Xue Bao ; 30(8): 790-6, 2003 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-14682251

RESUMO

The diverse plant proteinase inhibitors (PIs) genes from different plant species have been isolated and their products with one or more genes are targeted at different biochemical and physiological process with the insect, and well played a potent defensive role against insects and pathogens. The use of recombinant PIs and synergistic activation to protect plants has been incorporated in integrated pest management program. Though they may not replace the use of chemical pesticides in the near future, but effectively complement it. Currently, the role and mechanism of action for most of these inhibitors are being studied in detail. This article describes the structure of PIs genes, their regulation and expression, and discusses the strategy to develop transgenic plants against phytophagous insects.


Assuntos
Genes de Plantas/genética , Controle Biológico de Vetores/métodos , Doenças das Plantas/genética , Inibidores de Proteases/metabolismo , Animais , Regulação da Expressão Gênica de Plantas , Imunidade Inata/genética , Doenças das Plantas/parasitologia , Plantas/genética , Plantas/parasitologia , Plantas Geneticamente Modificadas
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