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1.
Khirurgiia (Mosk) ; (12): 91-95, 2018.
Artigo em Russo | MEDLINE | ID: mdl-30560852

RESUMO

AIM: To improve the outcomes in patients with chronic ruptures of the Achilles tendon followed by advanced diastase and reduced function of the gastrocnemius muscle by using of new surgical method. MATERIAL AND METHODS: The authors proposed a new method of Achilles tendon repair for diastase over 10 cm. It imposes transposition of the tendons of short fibular muscle and posterior tibial muscle. Schemes and stages of surgery are presented in figures. The authors described clinical example of patient with chronic rupture of the Achilles tendon, illustrations of surgical stages and the outcome. RESULTS: New surgical approach was applied in 16 patients. The control group consisted of 21 patients who underwent repair by Chernavsky, Krasnov. Clinical and biomechanical methods were used for evaluation. Functional myography and podography to assess the asymmetry of gait were performed. Our data confirmed the advantage of new approach.


Assuntos
Tendão do Calcâneo/cirurgia , Músculo Esquelético/transplante , Procedimentos Ortopédicos/métodos , Procedimentos Cirúrgicos Reconstrutivos/métodos , Traumatismos dos Tendões/cirurgia , Tendão do Calcâneo/lesões , Doença Crônica , Humanos , Ruptura , Resultado do Tratamento
5.
Biochemistry (Mosc) ; 64(8): 933-7, 1999 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-10498811

RESUMO

Reverse transcriptase (RT) preparations containing various molecular forms of the enzyme consisting of alpha- and/or beta-subunits have been isolated from E. coli cells transformed with plasmid pMF14 containing the Rous sarcoma virus (RSV) pol gene. The three possible dimeric forms of the enzyme demonstrated DNA polymerase activity, the relative activities of the alphaalpha, betabeta, and alphabeta forms being about 1:3:4. RNase H activity is associated with the betabeta and alphabeta dimers but not with the alphaalpha dimer. Comparison of the enzymic properties of the various dimers and dissociation--reassociation results suggest that the betabeta and alphabeta dimers of the RSV recombinant reverse transcriptase are similar to the corresponding virion RT forms.


Assuntos
Vírus do Sarcoma Aviário/enzimologia , DNA Polimerase Dirigida por RNA/isolamento & purificação , DNA Polimerase Dirigida por RNA/metabolismo , Centrifugação com Gradiente de Concentração , Cromatografia de Afinidade , Cromatografia DEAE-Celulose , Cromatografia por Troca Iônica , Clonagem Molecular , Dimerização , Escherichia coli , Cinética , DNA Polimerase Dirigida por RNA/química , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/metabolismo , Especificidade por Substrato , Moldes Genéticos
8.
Biokhimiia ; 60(6): 874-82, 1995 Jun.
Artigo em Russo | MEDLINE | ID: mdl-7544630

RESUMO

Preparations of Rous sarcoma virus reverse transcriptase isolated from a culture of E. coli HB101 (pMF14) and purified to homogeneity were used to study the steady state kinetics of DNA polymerization and inhibition of DNA-polymerase activity. DNA synthesis was examined using a system of poly(rA) as template, oligo(dT) as primer and dTTP as nucleotide substrate. Kinetic constants for steady state conditions were determined. The substrate initial velocity patterns point to an ordered mechanism which results in the formation of a ternary complex, in which the template-primer is the first to bind to the enzyme. Inhibition of the DNA-polymerase activity of the enzyme by various inhibitors was studied. Analysis of final products of the DNA-polymerase reaction revealed the presence of distribution syntheses of the DNA chain by the alpha alpha-subunit form of the enzyme.


Assuntos
Vírus do Sarcoma Aviário/enzimologia , DNA Polimerase Dirigida por DNA/metabolismo , DNA Polimerase Dirigida por RNA/metabolismo , Catálise , DNA Recombinante/biossíntese , Escherichia coli/genética , Cinética , Inibidores da Síntese de Ácido Nucleico , DNA Polimerase Dirigida por RNA/genética , Proteínas Recombinantes/antagonistas & inibidores , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Inibidores da Transcriptase Reversa
9.
Ortop Travmatol Protez ; (4): 35-8, 1991 Apr.
Artigo em Russo | MEDLINE | ID: mdl-1754172

RESUMO

On the basis of treatment and examination of 2675 patients with residual effects of poliomyelitis and consequences of the nervous system traumata are considered the functioning peculiarities of vascular system of the lower extremities in case of absence or abrupt decrease of pumping function of the skeletal muscles. There have been detected discrepancies between the reovasographic regional blood circulation pattern and the microcirculation state indexes in the tissues of the damaged extremity. They have studied the dynamics of the hemodynamics indexes in the process of preoperative preparation, during early and late postoperative periods as well as during remote postoperative periods. There is suggested the idea of correlation of the regional hemodynamics with the extremity supporting state.


Assuntos
Hemodinâmica/fisiologia , Perna (Membro)/irrigação sanguínea , Músculos/irrigação sanguínea , Paralisia/fisiopatologia , Poliomielite/fisiopatologia , Transferência Tendinosa/métodos , Humanos , Perna (Membro)/inervação , Hipotonia Muscular/etiologia , Hipotonia Muscular/fisiopatologia , Músculos/inervação , Paralisia/etiologia , Paralisia/cirurgia , Poliomielite/complicações , Período Pós-Operatório , Fatores de Tempo
13.
Biomed Sci ; 2(1): 49-53, 1991.
Artigo em Inglês | MEDLINE | ID: mdl-1717011

RESUMO

Enzyme preparations of Rous sarcoma virus (RSV) reverse transcriptase have been isolated from a culture of E. coli HB101(pMF14). The enzyme has been purified to homogeneity and been shown to consist of two subunits, of molecular mass 97.4 and 61.3 kDa, respectively. The optimum conditions for the DNA polymerase and RNAase H activities, fidelity of DNA synthesis on a homogeneous RNA template, and the inhibitory effect of azidothymidine triphosphate have been determined. Data on the use of RSV recombinant reverse transcriptase for cDNA synthesis are given.


Assuntos
DNA Polimerase Dirigida por DNA/metabolismo , DNA Polimerase Dirigida por RNA/química , Ribonuclease H/metabolismo , Vírus do Sarcoma Aviário/enzimologia , Replicação do DNA/efeitos dos fármacos , Relação Dose-Resposta a Droga , Concentração de Íons de Hidrogênio , Técnicas In Vitro , Cloreto de Magnésio/farmacologia , Cloreto de Potássio/farmacologia , DNA Polimerase Dirigida por RNA/isolamento & purificação , Proteínas Recombinantes/química , Rifamicinas/farmacologia , Fluoreto de Sódio/farmacologia , Temperatura , Zidovudina/farmacologia
15.
Biokhimiia ; 55(4): 586-94, 1990 Apr.
Artigo em Russo | MEDLINE | ID: mdl-1696135

RESUMO

Preparations of recombinant reverse transcriptase RSV were isolated from E. coli HB101/pMF14 cell cultures. The enzyme purified to homogeneity was shown to be made up of two subunits with molecular masses of 97 +/- 4 and 61 +/- 3 kDa. A comparison of enzymatic properties of recombinant transcriptase to those of the enzyme isolated from the RSV (Rauss sarcoma) virus demonstrated that in the preparations under study the recombinant reverse transcriptase exists in a subunit form, alpha beta, and may acquire a relatively stable configuration, alpha 2.


Assuntos
Vírus do Sarcoma Aviário/enzimologia , DNA Polimerase Dirigida por RNA/isolamento & purificação , Recombinação Genética , Vírus do Sarcoma Aviário/genética , Eletroforese em Gel de Poliacrilamida , Estabilidade Enzimática , Escherichia coli/enzimologia , Escherichia coli/genética , Temperatura Alta , Cinética , DNA Polimerase Dirigida por RNA/análise , DNA Polimerase Dirigida por RNA/genética , Moldes Genéticos
18.
Sov Med ; (8): 21-7, 1989.
Artigo em Russo | MEDLINE | ID: mdl-2814661

RESUMO

The authors analyse their many-year experience gained in the treatment of patients with foot deformities of various origins, carried out at the traumatology clinic of the Kuibyshev D. I. Ulyanov Medical Institute. New surgical techniques for the treatment of congenital talipes, valgus deformity of the first thumb combined with transverse platypodia, paralytic loose, paralytic pes planus and calcaneus are described. The causes of valgus deformities in the patients with infantile cerebral paralysis after achillotomy are discussed on the basis of EMG data. The described methods are superior to the routine ones.


Assuntos
Deformidades Adquiridas do Pé/cirurgia , Deformidades Congênitas do Pé/cirurgia , Adolescente , Artrodese , Criança , Pré-Escolar , Seguimentos , Humanos , Métodos , Fatores de Tempo
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