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1.
FEBS Lett ; 306(2-3): 185-8, 1992 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-1378793

RESUMO

Four 3'-mercapto-2',3'-dideoxynucleoside 5'-triphosphates (A, G, C and T) were tested as DNA chain terminator substrates for calf thymus alpha-DNA polymerase, E. coli DNA polymerase I Klenow fragment, terminal deoxynucleotidyl transferase and reverse transcriptases of AMV, HIV and MLV viruses. It was shown that the analogues selectively and irreversibly terminated DNA chain elongation by AMV and HIV reverse transcriptases and the terminal transferase. Other DNA polymerases tested did not use the nucleotide analogues as chain terminator substrate.


Assuntos
DNA Viral/biossíntese , HIV/efeitos dos fármacos , Inibidores da Transcriptase Reversa , Compostos de Sulfidrila/farmacologia , Sequência de Bases , HIV/enzimologia , Dados de Sequência Molecular , Inibidores da Síntese de Ácido Nucleico
4.
Nucleic Acids Symp Ser ; (18): 121-4, 1987.
Artigo em Inglês | MEDLINE | ID: mdl-3320974

RESUMO

The group of DNA polymerases was studied using some new nucleoside 5'-triphosphate analogs with termination substrate properties. Among DNA polymerases tested the least specific appeared reverse transcriptases of retroviruses and the most specific were DNA polymerases alpha type from high eucaryotes including mammalians.


Assuntos
DNA Polimerase Dirigida por DNA/metabolismo , Desoxirribonucleotídeos/síntese química , Animais , Vírus da Mieloblastose Aviária/enzimologia , Bovinos , DNA/biossíntese , Escherichia coli/enzimologia , Fígado/enzimologia , Ratos , Especificidade por Substrato , Fagos T/enzimologia , Timo/enzimologia
5.
Biochim Biophys Acta ; 868(2-3): 145-52, 1986 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-3021225

RESUMO

A number of nucleoside 5'-triphosphate analogs were tested with Escherichia coli DNA polymerase I and Klenow fragment of the enzyme, bacteriophage T4 DNA polymerase and calf thymus DNA polymerase alpha. It was shown that 3'-amino-2',3'-dideoxynucleoside 5'-triphosphates as well as a number of 3'-derivatives of dTTP(3'NH2) are able to terminate DNA synthesis catalyzed by each enzyme if the reaction is performed in the absence of natural substrates. ddNTP and dNTP(3'F) were found to be inactive with DNA polymerase alpha only, but araNTP(3'NH2) was inactive with E. coli DNA polymerase I. dTTP(3'N3), dGTP(3'N'3), dCTP(3'N3), araNTP(3'N3) and (alpha-thio)dTTP(3'F) were unable to inhibit any of the above-mentioned DNA polymerases, in contrast to reverse transcriptase, accessible to the most nucleotide analogs tested.


Assuntos
DNA Polimerase Dirigida por DNA/metabolismo , Nucleotídeos/metabolismo , DNA/biossíntese , DNA/metabolismo , Difosfatos/metabolismo , Hidrólise , Inibidores da Síntese de Ácido Nucleico , Nucleotídeos/farmacologia , Especificidade por Substrato
6.
FEBS Lett ; 207(2): 205-12, 1986 Oct 27.
Artigo em Inglês | MEDLINE | ID: mdl-2429865

RESUMO

dNTP(3'-OCH3), a 3'-O-methyl derivative of dNTP, is a chain terminator substrate for DNA synthesis catalyzed by AMV reverse transcriptase. The enzyme seems to be the only DNA polymerase susceptible to the inhibitor while all the other DNA polymerases tested are fully resistant to the nucleotide analog. The resistant polymerases are: E. coli DNA polymerase I, Klenow's fragment of DNA polymerase I, phage T4 DNA polymerase, calf thymus DNA polymerase alpha, rat liver DNA polymerase beta and calf thymus terminal deoxyribonucleotidyl transferase.


Assuntos
Desoxirribonucleotídeos/farmacologia , Inibidores da Transcriptase Reversa , Animais , Bovinos , DNA/biossíntese , DNA Polimerase I/antagonistas & inibidores , DNA Polimerase II/antagonistas & inibidores , DNA Polimerase Dirigida por DNA/metabolismo , Escherichia coli/enzimologia , Fígado/enzimologia , Inibidores da Síntese de Ácido Nucleico , Oligonucleotídeos/metabolismo , Ratos , Fagos T/enzimologia , Timo/enzimologia
7.
FEBS Lett ; 183(2): 275-8, 1985 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-2580738

RESUMO

It is shown that dNTP(3'F) are terminators of DNA synthesis and may serve as very effective tools for DNA sequencing with E.coli DNA polymerase I and AMV reverse transcriptase. The dNTP(3'F) are found to be chain terminator substrates for calf thymus terminal deoxyribonucleotidyl transferase but not for calf thymus DNA polymerase alpha. The optimal dNTP(3'F) concentration for DNA sequencing by DNA polymerase I is found to be an order of magnitude lower than that of ddNTPs. dNTP(3'F) produce a more clear sequence pattern than do ddNTPs.


Assuntos
Replicação do DNA/efeitos dos fármacos , Desoxirribonucleotídeos/farmacologia , Flúor/farmacologia , Animais , Sequência de Bases , Bovinos , DNA Nucleotidilexotransferase/metabolismo , DNA Polimerase I/metabolismo , Escherichia coli , DNA Polimerase Dirigida por RNA/metabolismo
8.
Nucleic Acids Res ; 12(3): 1671-86, 1984 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-6322115

RESUMO

It is shown that 2',3'-dideoxy-3'-aminonucleoside 5'-triphosphates with adenine, guanine, cytosine and thymine bases are effective inhibitors of DNA polymerase I, calf thymus DNA polymerase alpha and rat liver DNA polymerase beta. The effect of the above-mentioned compounds is markedly higher than corresponding action of the well-known DNA synthesis inhibitors arabinonucleoside 5'-triphosphates and 2',3'-dideoxynucleoside 5'-triphosphates. 2',3'-dideoxy-3'-aminonucleoside 5'-monophosphate residues incorporate into the 3'-terminus of the primer and terminate the DNA chain elongation. The possibility of using 2',3'-dideoxy-3'-aminonucleoside 5'-triphosphates as terminators for DNA sequencing by the polymerization method is demonstrated.


Assuntos
DNA Polimerase II/antagonistas & inibidores , DNA Polimerase I/antagonistas & inibidores , Replicação do DNA/efeitos dos fármacos , Desoxirribonucleotídeos/farmacologia , Animais , Sequência de Bases , Bovinos , Enzimas de Restrição do DNA , Escherichia coli/enzimologia , Cinética , Fígado/enzimologia , Ratos , Relação Estrutura-Atividade , Timo/enzimologia
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