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1.
Arch Virol ; 167(4): 1157-1162, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35258648

RESUMO

In this work, two new turnip mosaic virus (TuMV) strains (Canola-12 and Canola-14) overcoming resistance in canola (Brassica napus) were isolated from a B. napus sample that showed typical TuMV-like symptoms and was collected in the city of Gimcheon, South Korea, in 2020. The complete genome sequence was determined and an infectious clone was made for each isolate. Phylogenetic analysis indicated that the strains isolated from canola belonged to the World-B group. Both infectious clones, which used 35S and T7 promoters to drive expression, induced systemic symptoms in Nicotiana benthamiana and B. napus. To our knowledge, this is the first report of TuMV infecting B. napus in South Korea.


Assuntos
Brassica napus , Potyvirus , Células Clonais , DNA Complementar/genética , Filogenia , Doenças das Plantas , Potyvirus/genética
2.
Arch Virol ; 167(4): 1089-1098, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35258649

RESUMO

Perilla is an annual herb with a unique aroma and taste that has been cultivated in Korea for hundreds of years. It has been widely cultivated in many Asian and European countries as a food and medicinal crop. Recently, several viruses have been reported to cause diseases in perilla in Korea, including turnip mosaic virus (TuMV), which is known as a brassica pathogen due to its significant damage to brassica crops. In this study, we determined the complete genome sequences of two new TuMV isolates originating from perilla in Korea. Full-length infectious cDNA clones of these two isolates were constructed, and their infectivity was tested by agroinfiltration of Nicotiana benthamiana and sap inoculation of Chinese cabbage and radish plants. In addition, we analyzed the phylogenetic relationship of six new Korean TuMV isolates to members of the four major groups. We also used RDP4 software to conduct recombination analysis of recent isolates from Korea, which provided new insight into the evolutionary relationships of Korean isolates of TuMV.


Assuntos
Perilla frutescens , Células Clonais , Filogenia , Doenças das Plantas , Potyvirus
3.
Phytopathology ; : PHYTO04210172R, 2022 May 02.
Artigo em Inglês | MEDLINE | ID: mdl-35113673

RESUMO

Three infectious clones of radish mosaic virus (RaMV) were generated from isolates collected in mainland Korea (RaMV-Gg) and Jeju Island (RaMV-Aa and RaMV-Bb). These isolates differed in sequences and pathogenicity. Examination of the wild-type isolates and reassortants between the genomic RNA1 and RNA2 of these three isolates revealed that severe symptoms were associated with RNA1 of isolates Aa or Gg causing systemic necrosis in Nicotiana benthamiana, or with RNA1 of isolate Bb for induction of veinal necrosis and severe mosaic symptoms in radish. Reverse transcription, followed by quantitative real-time PCR (Q-RT-PCR), results from infected N. benthamiana confirmed that viral RNA2 accumulation level was correlated to RaMV necrosis-inducing ability, and that the RNA2 accumulation level was mostly dependent on the origin of RNA1. However, in radish, Q-RT-PCR results showed more similar viral RNA2 accumulation levels regardless of the ability of the isolate to induce necrosis. Phylogenetic analysis of genomic RNAs sequence including previously characterized isolates from North America, Europe, and Asia suggest possible recombination within RNA1, while analysis of concatenated RNA1+RNA2 sequences indicates that reassortment of RNA1 and RNA2 has been more important in the evolution of RaMV isolates than recombination. Korean isolate Aa is a potential reassortant between isolates RaMV-J and RaMV-TW, while isolate Bb might have evolved from reassortment between isolates RaMV-CA and RaMV-J. The Korean isolates were shown to also be able to infect Chinese cabbage, raising concerns that RaMV may spread from radish fields to the Chinese cabbage crop in Korea, causing further economic losses.

5.
Antimicrob Agents Chemother ; 66(2): e0168421, 2022 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-34871098

RESUMO

Delpazolid, an oxazolidinone, has been studied in non-clinical studies of efficacy and toxicity and Phase 1 clinical studies. Delpazolid has in vitro activity against Gram-positive bacteria, including Mycobacterium tuberculosis. This study evaluated the bactericidal activity, safety, and pharmacokinetics of delpazolid in patients with pulmonary tuberculosis (TB). Seventy-nine subjects, aged 19 to 75 years with newly diagnosed smear-positive TB with no prior treatment for the current episode and no confirmed resistance to rifampin or isoniazid, were randomized to receive delpazolid 800 mg once a day (QD), 400 mg twice a day (BID), 800 mg BID or 1,200 mg QD or an active control of isoniazid, rifampin, pyrazinamide, and ethambutol (HRZE) or linezolid 600 mg BID. The primary endpoint was the average daily reduction in log transformed bacterial load, assessed on 7H11 solid-media culture, from days 0 to 14. The average daily decline in log-CFU was 0.044 ± 0.016, 0.053 ± 0.017, 0.043 ± 0.016, and 0.019 ± 0.017, for the delpazolid 800 mg QD, 400 mg BID, 800 mg BID, and the 1,200 mg QD groups, respectively. The average daily decline in log-CFU was 0.192 ± 0.028 for the HRZE group and 0.154 ± 0.023 for the linezolid 600 mg BID group. Three serious adverse events (SAE) were reported, one each in the delpazolid 400 mg BID group (death due to worsening of TB at day 2), the HRZE group (hospitalization due to pleural effusion) and the linezolid group (hyperkalemia); none of the SAEs were assessed as related to study drugs. This study has been registered at ClinicalTrials.gov with registration number NCT02836483.


Assuntos
Mycobacterium tuberculosis , Oxazolidinonas , Tuberculose Pulmonar , Adulto , Idoso , Antituberculosos/uso terapêutico , Quimioterapia Combinada , Humanos , Isoniazida/uso terapêutico , Pessoa de Meia-Idade , Oxazolidinonas/farmacocinética , Oxazolidinonas/uso terapêutico , Pirazinamida/uso terapêutico , Escarro/microbiologia , Tuberculose Pulmonar/tratamento farmacológico , Tuberculose Pulmonar/microbiologia , Adulto Jovem
6.
Mol Cell Probes ; 61: 101789, 2022 02.
Artigo em Inglês | MEDLINE | ID: mdl-34965481

RESUMO

Apple scar skin viroid (ASSVd), of the genus Apscaviroid, causes serious pome fruit diseases, such as apple scar skin, dapple apple, pear rusty skin, pear fruit crinkle, and pear dimple fruit. This study aimed at establishing a sensitive and accurate method for quantification of ASSVd in apple leaves and plantlets using a reverse transcription droplet digital polymerase chain reaction (RT-ddPCR) assay. The specificity was analyzed using other apple viruses, and the negative amplification of the cross-reaction assay demonstrated the high specificity of RT-ddPCR. The detection limit of ASSVd by RT-ddPCR was 1.75 × 102 copies/µL (0.14 concentration), and the sensitivity was ten-fold higher than that of RT-qPCR. Similarly, positive detection in apple plantlet samples by RT-ddPCR was higher than that by RT-qPCR. The RT-ddPCR assay represents a promising alternative for accurate quantitative detection and diagnosis of ASSVd infection in ASSVd-free certification programs.


Assuntos
Malus , Viroides , Doenças das Plantas , Vírus de Plantas , RNA Viral/genética , Reação em Cadeia da Polimerase em Tempo Real/métodos , Transcrição Reversa , Sensibilidade e Especificidade , Viroides/genética
7.
J Transcult Nurs ; 33(1): 49-56, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34130552

RESUMO

INTRODUCTION: Married immigrant women experience cultural conflicts due to different values, and customs. This study examined the factors associated with the acculturation and acculturative stress of Asian immigrant women in South Korea. METHOD: A cross-sectional descriptive study was performed. A total of 142 participants were recruited from Incheon Multicultural Centers in 2016 using a convenience sampling method. Acculturation and acculturative stress were measured by Suinn-Lew Self-Identity Acculturation Scale and Social Attitudinal Familial Environment. The data were analyzed using analysis of variance and multiple regression analysis. RESULTS: Time since immigration was negatively associated with the level of acculturation, while alcohol consumption (p < .050). Some demographic variables (age, religion, anxiety, and time since immigration) were associated with acculturative stress (p < .050). DISCUSSION: These findings can help health care providers to increase their awareness and be more sensitive when providing services to these women.


Assuntos
Aculturação , Emigrantes e Imigrantes , Estudos Transversais , Feminino , Humanos , Masculino , República da Coreia , Estresse Psicológico/complicações
8.
Int Nurs Rev ; 2021 Dec 08.
Artigo em Inglês | MEDLINE | ID: mdl-34881445

RESUMO

AIMS: This study aimed to investigate the differences in practices, policies, and perceptions of nurses regarding work-related utilization of smartphones in acute-care settings. BACKGROUND: Mobile devices utilizing emerging technology are widely used in acute-care settings; however, concerns such as potential distraction, improper use, and negative impact on the nursing image in clinical practice remain valid. METHODS: Nurse managers (n = 8) and nurses (n = 181) were enrolled from eight academic tertiary hospitals that have comprehensive electronic medical record systems and mobile versions. Between October 2018 and February 2019, participants completed a questionnaire designed to explore their use of smartphones. The reporting guide for self-administered surveys of clinicians was applied. RESULTS: Approximately 80% of nurses carried personal smartphones while working, with 70% using their devices for work. The prevalence of work-related smartphone use ranged from 3% to 43% by functionality, which was lower than that estimated by managers. Frequent uses included taking pictures/videos and internet browsing. Nurses were more positive than managers about the benefits of smartphone use and less burdened by related concerns. Novice and junior nurses were more optimistic than senior nurses. Only one hospital had a policy on nurses' use of personal devices at work. CONCLUSION: Two unmet needs in the current clinical information system were identified: information supporting task-related knowledge at the bedside and security of data capture and communication. IMPLICATION FOR NURSING POLICY: The unintended perception gaps between nurses and managers regarding work-related smartphone use can be closed by nursing leadership. Unmet nursing informatics, particularly for information-seeking purposes, can be addressed in the context of quality assurance. Nurse leaders can advocate secure and proper use of smartphones in clinical practice.

9.
Stud Health Technol Inform ; 284: 39-40, 2021 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-34920464

RESUMO

This study used topic modeling to analyse key topics of nursing handoff research. Six topics were identified. The findings indicate that future studies should implement the standardization of handoff tools and the use of bedside handoff, and evaluate their effects on patient safety outcomes.


Assuntos
Pesquisa em Enfermagem , Transferência da Responsabilidade pelo Paciente , Humanos
10.
Stud Health Technol Inform ; 284: 71-73, 2021 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-34920475

RESUMO

We analyzed the spatial and temporal patterns of inpatient falls in a tertiary hospital. Data were obtained from the adverse-event self-reporting system and the electronic nursing record system of the hospital. Through chart reviews we reclassified the cases. Most falls occurred by the bedside, followed by the restroom and hallway, and they occurred most often between 2 a.m. and 6 a.m. These findings indicate when and where nurses should be most alert about falls.


Assuntos
Eletrônica , Pacientes Internados , Humanos , Centros de Atenção Terciária
11.
Stud Health Technol Inform ; 284: 228-230, 2021 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-34920515

RESUMO

Patient safety is a fundamental aspect of the quality of healthcare and there is a growing interest in improving safety among healthcare stakeholders in many countries. The Korean government recognized that patient safety is a threat to society following several serious adverse events, and so the Ministry of Health and Welfare of the Korean government set up the Patient Safety Act in January 2015. This study analyzed text data on patient safety collected from web-based, user-generated documents related to the legislation to see if they accurately represent the specific concerns of various healthcare stakeholders. We adopted the unsupervised natural language processing method of probabilistic topic modeling and also Latent Dirichlet Allocation. The results showed that text data are useful for inferring the latent concerns of healthcare consumers, providers, government bodies, and researchers as well as changes therein over time.


Assuntos
Governo , Segurança do Paciente , Atenção à Saúde , Humanos , Internet
12.
Plant Pathol J ; 37(6): 632-640, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34897254

RESUMO

Cucumber mosaic virus (CMV) and Pepper mild mottle virus (PMMoV) causes severe economic loss in crop productivity of both agriculture and horticulture crops in Korea. The previous surveys showed that naturally available biopolymer material - chitosan (CS), which is from shrimp cells, reduced CMV accumulation on pepper. To improve the antiviral activity of CS, it was synthesized to form phosphate cross-linked chitosan (PCS) and compared with the original CS. Initially, the activity of CS and PCS (0.01%, 0.05%, and 0.1% concentration) compound against PMMoV infection and replication was tested using a half-leaf assay on Nicotiana glutinosa leaves. The total number of local lesions represented on a leaf of N. glutinosa were counted and analyzed with phosphate buffer treated leaves as a negative control. The leaves treated with a 0.1% concentration of CS or PCS compounds exhibited an inhibition effect by 40-75% compared with the control leaves. The same treatment significantly reduced about 40% CMV accumulation measured by double antibody sandwich enzyme-linked immunosorbent assay and increased the relative expression levels of the NPR1, PR-1, cysteine protease inhibitor gene, LOX, PAL, SRC2, CRF3 and ERF4 genes analyzed by quantitative reverse transcriptase-polymerase chain reaction, in chili pepper plants.

13.
JMIR Med Inform ; 9(11): e26456, 2021 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-34626168

RESUMO

BACKGROUND: Patient falls are a common cause of harm in acute-care hospitals worldwide. They are a difficult, complex, and common problem requiring a great deal of nurses' time, attention, and effort in practice. The recent rapid expansion of health care predictive analytic applications and the growing availability of electronic health record (EHR) data have resulted in the development of machine learning models that predict adverse events. However, the clinical impact of these models in terms of patient outcomes and clinicians' responses is undetermined. OBJECTIVE: The purpose of this study was to determine the impact of an electronic analytic tool for predicting fall risk on patient outcomes and nurses' responses. METHODS: A controlled interrupted time series (ITS) experiment was conducted in 12 medical-surgical nursing units at a public hospital between May 2017 and April 2019. In six of the units, the patients' fall risk was assessed using the St. Thomas' Risk Assessment Tool in Falling Elderly Inpatients (STRATIFY) system (control units), while in the other six, a predictive model for inpatient fall risks was implemented using routinely obtained data from the hospital's EHR system (intervention units). The primary outcome was the rate of patient falls; secondary outcomes included the rate of falls with injury and analysis of process metrics (nursing interventions that are designed to mitigate the risk of fall). RESULTS: During the study period, there were 42,476 admissions, of which 707 were for falls and 134 for fall injuries. Allowing for differences in the patients' characteristics and baseline process metrics, the number of patients with falls differed between the control (n=382) and intervention (n=325) units. The mean fall rate increased from 1.95 to 2.11 in control units and decreased from 1.92 to 1.79 in intervention units. A separate ITS analysis revealed that the immediate reduction was 29.73% in the intervention group (z=-2.06, P=.039) and 16.58% in the control group (z=-1.28, P=.20), but there was no ongoing effect. The injury rate did not differ significantly between the two groups (0.42 vs 0.31, z=1.50, P=.134). Among the process metrics, the risk-targeted interventions increased significantly over time in the intervention group. CONCLUSIONS: This early-stage clinical evaluation revealed that implementation of an analytic tool for predicting fall risk may to contribute to an awareness of fall risk, leading to positive changes in nurses' interventions over time. TRIAL REGISTRATION: Clinical Research Information Service (CRIS), Republic of Korea KCT0005286; https://cris.nih.go.kr/cris/search/detailSearch.do/16984.

14.
Comput Inform Nurs ; 40(2): 95-103, 2021 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-34412084

RESUMO

Advances in bibliometrics and co-citation analysis provide the opportunity to analyze quantitatively the large amount of nursing research used in evidence-based nursing. Numerous nursing researchers have attempted to obtain evidence that using evidence-based nursing improves the quality of nursing practices. However, little is known about how these efforts comply with the rigorous methods required for a systematic review in a subject area. This study explored the comprehensiveness of systematic reviews in four guidelines for preventing inpatient falls using bibliometrics and a co-citation network technique. Citations (n = 659) and 9417 unique bibliographic records written by 6537 authors were collected from the guidelines in January 2020. The results showed none of the references spanned all four authoring bodies, whereas only 0.1% and 4.7% of the references spanned three and two of the authoring bodies, respectively. The co-citation analysis revealed differences in the scope and primary concerns among the development groups, even in the same setting, with differences in the highly influential articles and authors. These findings imply that although the systematic reviews of guidelines on fall prevention were of good quality, there remain areas for improvement in terms of harmonizing the selection of bibliographic citations comprehensively.


Assuntos
Acidentes por Quedas , Enfermagem Baseada em Evidências , Acidentes por Quedas/prevenção & controle , Bibliometria , Humanos , Pacientes Internados , Guias de Prática Clínica como Assunto , Revisões Sistemáticas como Assunto
15.
Mol Cell Probes ; 58: 101746, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-34102255

RESUMO

Peach latent mosaic viroid (PLMVd) represents a continuing threat to peach tree production worldwide. In this study, a sensitive and accurate quantification of PLMVd in peach leaves was established using a reverse transcription droplet digital polymerase chain reaction (RT-ddPCR) assay. The quantitative linearity, accuracy, and sensitivity of RT-ddPCR for the detection of PLMVd were comparatively assessed to those of reverse-transcription real-time quantitative polymerase chain reaction (RT-qPCR) assay. The specificity assay shows no amplification in major peach viruses, apple chlorotic leaf spot virus and prunus necrotic ring spot virus and negative control. Furthermore, the levels of PLMVd transcripts determined using RT-ddPCR and RT-qPCR showed a high degree of linearity and quantitative correlation. Our results also indicated that the RT-ddPCR assay is at least two-fold more sensitive than qPCR and could therefore, be used to detect PLMVd in field samples. Moreover, optimization of RT-ddPCR was found to enhance the sensitivity of PLMVd detection in the peach leaf samples with low viral loads. In summary, the established RT-ddPCR assay represents a promising alternative method for the precise quantitative detection of PLMVd; it would be particularly applicable for diagnosing PLMVd infections in plant quarantine inspection and PLMVd-free certification program.


Assuntos
Prunus , Transcrição Reversa , Doenças das Plantas , Vírus de Plantas , RNA Viral/genética , Reação em Cadeia da Polimerase em Tempo Real
16.
Work ; 2021 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-34120924

RESUMO

BACKGROUND: Despite the benefits of using electronic medical record (EMR) systems, existing studies show that many healthcare providers are uncertain regarding their usability. The usability issues of these systems decrease their efficiency, discourage clinicians, and cause dissatisfaction among patients, which may result in safety risks and harm. OBJECTIVE: The aim of this study was to collect and analyze EMR system usability problems from actual users. Practical user interface guidelines were presented based on the medical practices of these users. METHODS: Employing an online questionnaire with a seven-point Likert scale, usability issues of EMR systems were collected from 200 emergency department healthcare providers (103 physicians (medical doctors) and 97 nurses) from South Korea. RESULTS: The most common usability problem among the physicians and nurses was generating in-patient selection. This pertained to the difficulty in finding the required information on-screen because of poor visibility and a lack of distinctiveness. CONCLUSIONS: The major problems of EMR systems and their causes were identified. It is recommended that intensive visual enhancement of EMR system interfaces should be implemented to support user tasks. By providing a better understanding of the current usability problems among medical practitioners, the results of this study can be useful for developing EMR systems with increased effectiveness and efficiency.

17.
J Korean Acad Nurs ; 51(2): 232-244, 2021 Apr.
Artigo em Coreano | MEDLINE | ID: mdl-33993128

RESUMO

PURPOSE: This study aimed to identify the modifiable factors affecting breastfeeding planning and duration among healthy mothers and their use of breastfeeding information resources. METHODS: A cross-sectional survey was conducted in a community setting. Four hundreds participants were recruited at five pediatric clinics and three community health centers located in Paju-si and Goyang-si, Gyeonggi-do, between January and May 2019. Based on the breastfeeding decision-making model, driven by Martens and Young's work, the survey items consisted of demographics, childbirth and breastfeeding characteristics, and breastfeeding information resources. In the analysis, 389 responses were used in the t-test, ANOVA, and logistic regression. Information resource networks were compared before and after childbirth including a subgroup analysis depending on the breastfeeding duration. RESULTS: The modifiable factors affecting breastfeeding planning and duration were antenatal and postpartum breastfeeding education and the provision of information in the hospital. The frequency of Internet use and websites visited were notable and potentially modifiable factors, which were also observed in the networks showing different relationship patterns according to participant subgroups and times. The childbirth event increased the centralization of the network in the planned group, while the network of the non-planned group was more diffused after childbirth. The network of the short-term breastfeeding group was characterized by a more centralized pattern and the resources of high betweenness centrality than the long-term group. CONCLUSION: Breastfeeding education is a consistent factor that affects breastfeeding behavior. A well-designed internet-based approach would be an effective nursing intervention to meet the needs of women seeking breastfeeding information and changing their behaviors.


Assuntos
Aleitamento Materno/estatística & dados numéricos , Comportamento de Busca de Informação , Mães/psicologia , Rede Social , Adulto , Aleitamento Materno/psicologia , Estudos Transversais , Feminino , Humanos , Lactente , Recém-Nascido , Intenção , Mães/educação , Mães/estatística & dados numéricos , Período Pós-Parto , Gravidez , Inquéritos e Questionários
18.
Medicine (Baltimore) ; 100(13): e25276, 2021 Apr 02.
Artigo em Inglês | MEDLINE | ID: mdl-33787612

RESUMO

ABSTRACT: Clinical information systems (CISs) that do not consider usability and safety could lead to harmful events. Therefore, we aimed to develop a safety and usability guideline of CISs that is comprehensive for both users and developers. And the guideline was categorized to apply actual clinical workflow and work environment.The guideline components were extracted through a systematic review of the articles published between 2000 and 2015, and existing CIS safety and/or usability design guidelines. The guideline components were categorized according to clinical workflow and types of user interface (UI). The contents of the guideline were evaluated and validated by experts with 3 specialties: medical informatics, patient safety, and human engineering.Total 1276 guideline components were extracted through article and guideline review. Of these, 464 guideline components were categorized according to 5 divisions of the clinical workflow: "Data identification and selection," "Document entry," "Order entry," "Clinical decision support and alert," and "Management". While 521 guideline components were categorized according to 4 divisions of UI: UIs related to information process steps, "Perception," "Recognition," "Control," and "Feedback". We developed a guideline draft with 219 detailed guidance for clinical task and 70 for UI. Overall appropriateness and comprehensiveness were proven to achieve more than 90% in experts' survey. However, there were significant differences among the groups of specialties in the judgment of appropriateness (P < .001) and comprehensiveness (P = .038).We developed and verified a safety and usability guideline for CIS that qualifies the requirements of both clinical workflows and usability issues. The developed guideline can be a practical tool to enhance the usability and safety of CISs. Further validation is required by applying the guideline for designing the actual CIS.


Assuntos
Sistemas de Apoio a Decisões Clínicas/normas , Aplicações da Informática Médica , Interface Usuário-Computador , Ergonomia , Humanos , Erros Médicos/prevenção & controle , Segurança do Paciente , Fluxo de Trabalho
19.
Mol Cell Probes ; 57: 101727, 2021 06.
Artigo em Inglês | MEDLINE | ID: mdl-33789127

RESUMO

Tomato spotted wilt virus (TSWV) is economically important in Korea as it causes significant losses to a wide range of important ornamental and vegetable crops. Therefore, a rapid detection method is imperative for TSWV diagnosis. Specific primers and probes were designed based on the conserved sequences of the TSWV coat protein gene. In this study, an isothermal reverse transcription recombinase polymerase amplification (RT-RPA) assay, combined with lateral flow strips (LFS), was established for rapid detection of TSWV in pepper infected leaves. The RT-RPA reaction was performed at an optimal condition of 38 °C for 10 min and an LFS incubation time of approximately 5 min. There was no cross-reactivity with other viruses infecting pepper such as cucumber mosaic virus, pepper mottle virus, pepper mild mottle virus, and broad bean wilt virus 2, thus confirming the specificity of RT-RPA-LFS. The sensitivity of the RT-RPA assay was similar to that of RT-PCR, and RT-RPA-LFS was successfully applied to detect TSWV in the pepper samples collected from the field. Thus, RT-RPA-LFS assay might be a promising candidate for quick diagnosis of TSWV-infected pepper plants.


Assuntos
Tospovirus , Primers do DNA , Folhas de Planta , Recombinases/genética , Transcrição Reversa , Tospovirus/genética
20.
Jpn J Nurs Sci ; 18(2): e12403, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33448157

RESUMO

AIM: This study was carried out to analyze nursing care provided to patients on high and low fall-risk days and to evaluate the effectiveness of nursing care in terms of preventing falls. METHODS: A retrospective review of medical records was conducted for patients admitted to a tertiary hospital in Korea. General and clinical information, fall occurrences, Hendrich II Fall Risk Model (HFRM II) fall-risk assessment scores, nursing care related to fall prevention, and medications administered were extracted. RESULTS: Data from 43,267 days of records for 11,718 patients were analyzed. Nursing assessment, intervention, and administration of medication were provided more frequently on high fall-risk days than on low fall-risk days. Analysis performed on the entire cohort days showed fall occurrence was significantly associated with infrequent mobility assessment and greater usage of anti-anxiety agents. On high fall-risk days, fall occurrence was related to less restraint assessment and greater usage of vessel dilatators. CONCLUSIONS: The implementation of risk-targeted interventions for fall prevention based on fall-risk assessment is needed. For general fall prevention, assessment of patients' mobility should be strengthened. For high fall-risk patients, it may be more effective for nurses to focus on assessing restraints, evaluating medication records, and withdrawing medications related to falls.


Assuntos
Acidentes por Quedas , Registros de Enfermagem , Acidentes por Quedas/prevenção & controle , Humanos , República da Coreia , Estudos Retrospectivos , Medição de Risco , Análise de Sobrevida , Centros de Atenção Terciária
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