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1.
J Microbiol Biotechnol ; 29(7): 1155-1164, 2019 Jul 28.
Artigo em Inglês | MEDLINE | ID: mdl-31280524

RESUMO

Lichens contain diverse bioactive secondary metabolites with various chemical and biological properties, which have been widely studied. However, details of the inhibitory mechanisms of their secondary metabolites against influenza A virus (IAV) have not been documented. Here, we investigated the antiviral effect of lichen extracts, obtained from South Korea, against IAV in MDCK cells. Of the lichens tested, Nipponoparmelia laevior (LC24) exhibited the most potent inhibitory effect against IAV infection. LC24 extract significantly increased cell viability, and reduced apoptosis in IAV-infected cells. The LC24 extract also markedly reduced (~ 3.2 logfold) IAV mRNA expression after 48 h of infection. To understand the antiviral mechanism of LC24 against IAV, proteomic (UPLC-HDMSE) analysis was performed to compare proteome modulation in IAV-infected (V) vs. mock (M) and LC24+IAV (LCV) vs. V cells. Based on Ingenuity Pathway Analysis (IPA), LC24 inhibited IAV infection by modulating several antiviral-related genes and proteins (HSPA4, HSPA5, HSPA8, ANXA1, ANXA2, HIF-1α, AKT1, MX1, HNRNPH1, HNRNPDL, PDIA3, and VCP) via different signaling pathways, including HIF-1α signaling, unfolded protein response, and interferon signaling. These molecules were identified as the specific biomarkers for controlling IAV in vitro and further confirmation of their potential against IAV in vivo is required. Our findings provide a platform for further studies on the application of lichen extracts against IAV.


Assuntos
Antivirais/farmacologia , Vírus da Influenza A Subtipo H1N1/efeitos dos fármacos , Líquens/química , Infecções por Orthomyxoviridae/metabolismo , Animais , Antivirais/metabolismo , Sobrevivência Celular/efeitos dos fármacos , Cães , Líquens/metabolismo , Células Madin Darby de Rim Canino , Infecções por Orthomyxoviridae/virologia , Proteômica , RNA Mensageiro/metabolismo , República da Coreia , Transdução de Sinais/efeitos dos fármacos , Proteínas Virais/genética
2.
Food Funct ; 10(8): 4636-4648, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31290903

RESUMO

This study aimed to evaluate the potentially beneficial effects of alginate oligosaccharide (AOS) on the modulation of immuno-metabolic pathways in high-fat-diet (HFD)-induced obese zebrafish and the underlying mechanism. AOS showed a marked anti-obesity effect in that it reduced body weight, BMI, and the blood glucose level. To understand the mechanisms of action of AOS, comparative proteomics was performed through UPLC-HDMSE analysis between HFD vs. normal diet (NFD) and HFD + AOS vs. HFD. Among 146 proteins differentially modulated by AOS in HFD-induced obesity zebrafish, STOML2 (Stomatin-like protein 2) was selected as a specific biomarker. AOS suppressed obesity and pathophysiological disorders in HFD-fed zebrafish by modulating lipid metabolism, suppressing inflammation, downregulating apoptosis-related genes, and improving immune function by inhibiting STOML2. Our results suggest that STOML2 can serve as a platform for further studies to discover novel treatments for metabolic disorders. AOS might be useful as a dietary health supplement, especially for reducing obesity.


Assuntos
Alginatos/administração & dosagem , Obesidade/tratamento farmacológico , Oligossacarídeos/administração & dosagem , Peixe-Zebra/metabolismo , Animais , Dieta Hiperlipídica/efeitos adversos , Modelos Animais de Doenças , Feminino , Humanos , Metabolismo dos Lipídeos/efeitos dos fármacos , Masculino , Obesidade/etiologia , Obesidade/metabolismo , Peixe-Zebra/genética , Peixe-Zebra/crescimento & desenvolvimento
3.
Fish Shellfish Immunol ; 88: 244-253, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30802632

RESUMO

Seasonal temperature has a major influence on the infectivity of pathogens and the host immune system. Viral hemorrhagic septicemia virus (VHSV) is one such pathogen that only causes the mortality of fish at low temperatures. This study aims to discover the host defense mechanism and pathway for resistance to VHSV at higher temperatures. We first observed the VHSV infection patterns at low and higher temperatures in fathead minnow (FHM) cells (20 °C and 28 °C) and zebrafish (15 °C and 25 °C). In comparison to the 20 °C infection, FHM cells infected at 28 °C showed decreased apoptosis, increased cell viability, and reduced VHSV N gene expression. In zebrafish, infection at 25 °C caused no mortality and significantly reduced the N gene copy number in comparison to infection at 15 °C. To explore the antiviral infection mechanisms induced by high temperature in vitro and in vivo, the changes in the proteomic profile were measured through UPLC-MSE analysis. ACADL, PTPN6, TLR1, F7, A2M, and GLI2 were selected as high temperature-specific biomarkers in the FHM cell proteome; and MYH9, HPX, ANTXR1, APOA1, HBZ, and MYH7 were selected in zebrafish. Increased immune response, anticoagulation effects, and the formation of lymphocytes from hematopoietic stem cells were analyzed as functions that were commonly induced by high temperature in vitro and in vivo. Among these biomarkers, GLI2 was predicted as an upstream regulator. When treated with GANT58, a GLI-specific inhibitor, cell viability was further reduced due to GLI2 inhibition during VHSV infection at varying temperatures in FHM cells, and the mortality in zebrafish was induced earlier at the low temperature. Overall, this study discovered a new mechanism for VHSV infection in vitro and in vivo that is regulated by GLI2 protein.


Assuntos
Cyprinidae/virologia , Septicemia Hemorrágica Viral/virologia , Novirhabdovirus , Temperatura , Peixe-Zebra/virologia , Animais , Apoptose , Sobrevivência Celular , Células Cultivadas , Expressão Gênica , Septicemia Hemorrágica Viral/mortalidade , Proteoma , Piridinas/farmacologia , Tiofenos/farmacologia , Peixe-Zebra/genética , Peixe-Zebra/metabolismo , Proteína Gli2 com Dedos de Zinco/genética , Proteína Gli2 com Dedos de Zinco/metabolismo
4.
J Food Prot ; 81(12): 1997-2002, 2018 12.
Artigo em Inglês | MEDLINE | ID: mdl-30476442

RESUMO

Prompt and inexpensive detection of hepatitis A virus (HAV) is essential to control acute hepatitis outbreaks associated with the consumption of contaminated raw or minimally processed food. In this study, various carbohydrate-binding lectins, including concanavalin A (Con A), wheat germ agglutinin, and soybean agglutinin, were compared for their binding affinity to HAV. Con A, which showed significantly higher binding affinity than other lectins, was used to develop an alternative and affordable method to conventional antibody-linked immunomagnetic separation prior to detection of HAV using reverse transcriptase PCR. This method, Con A-linked immunomagnetic separation combined with reverse transcriptase PCR, can detect HAV at a dilution concentration of 10-4 of the virus stock (titer: 104 median tissue culture infective dose per mL), indicating that Con A could be a promising candidate for concentrating HAV.


Assuntos
Concanavalina A , Contaminação de Alimentos/análise , Vírus da Hepatite A , Separação Imunomagnética/métodos , Concanavalina A/metabolismo , Surtos de Doenças , Vírus da Hepatite A/isolamento & purificação , Reação em Cadeia da Polimerase Via Transcriptase Reversa
5.
Fish Shellfish Immunol ; 71: 220-229, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29017947

RESUMO

Viral hemorrhagic septicemia virus (VHSV) is one of the most serious viral pathogen that infects farmed fish. In this study, we measured the replication of VHSV increased steadily at 9, 24, 72, and 120 h after infection and progression of necrosis was observed at 72 hpi. We performed transcriptomic and metabolomics profiling of kidney tissues collected at each infection time using Illumina HiSeq2000 and ultra-performance liquid chromatography/quadrupole time-of-flight mass spectroscopy to investigate the mechanisms of VHSV infection in the kidneys of olive flounder. A total of 13,862 mRNA molecules and 72 metabolites were selected to identify the mechanisms of infection and they were integrated using KEGG pathway database. Six KEGG metabolic pathways, including carbohydrate metabolism, amino acid metabolism, lipid metabolism, transport and catabolism, metabolism of cofactors and vitamins, and energy metabolism, were significantly suppressed, whereas the immune system was activated by VHSV infection. A decrease in levels of amino acids such as valine, leucine, and isoleucine, as well as in their derivative carnitines, was observed after VHSV infection. In addition, an increase in arachidonic acid level was noted. Integrated analysis of transcriptome and metabolome using KEGG pathway database revealed four types of responses in the kidneys of olive flounder to VHSV infection. Among these, the mechanisms related to the immune system and protein synthesis were activated, whereas ATP synthesis and the antioxidant system activity were suppressed. This is the first study describing the mechanisms of metabolic responses to VHSV infection in olive flounder. The results suggest that the suppression of ATP synthesis and antioxidant systems, such as glutathione and peroxisome signaling, could be the cause of necrosis, whereas the activation of the immune system could result in the inflammation of kidney tissue in VHSV-infected olive flounder.


Assuntos
Doenças dos Peixes/imunologia , Linguados/genética , Linguados/imunologia , Septicemia Hemorrágica Viral/imunologia , Metaboloma/imunologia , Transcriptoma/imunologia , Animais , Linguados/metabolismo , Linguado/imunologia , Rim/imunologia , Novirhabdovirus/fisiologia , Fatores de Tempo
6.
Planta Med ; 82(16): 1425-1430, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-27454708

RESUMO

Hyperforin, a major active compound of St. John's wort extract, affects estrogenic activity. In this study, the compound evoked estrogen response element-dependent luciferase activity and cell proliferation in MCF-7 cells. Hyperforin-induced cell proliferation was significantly inhibited by the estrogen receptor antagonist ICI 182,780. These results suggested that hyperforin had estrogenic and cell proliferation activities, which were stimulated via the estrogen receptor. Compared to 17ß-estradiol, hyperforin showed significantly lower estrogenic activity and cell proliferation. The mechanism underlying the estrogenic activity of hyperforin was unknown, therefore, in this study, for the first time, the expression and post-translational modification of proteins were determined and compared among control, 17ß-estradiol-treated, and hyperforin-treated cells using proteomic techniques. A total of 453 proteins were identified, of which 282 proteins were significantly modulated in hyperforin-treated cells compared to 17ß-estradiol-treated cells. Ingenuity pathway analysis also demonstrated that hyperforin treatment induced less cell proliferation than 17ß-estradiol by downregulating estrogen receptor 1. Protein network analysis showed that cell proliferation was regulated mainly by cyclin D1 and extracellular signal-regulated kinases. In conclusion, although, hyperforin exhibited lower estrogenic activity than 17ß-estradiol, the compound induced lower levels of cancer cell proliferation in vitro.


Assuntos
Estrogênios/análise , Floroglucinol/análogos & derivados , Terpenos/química , Proliferação de Células/efeitos dos fármacos , Humanos , Células MCF-7 , Floroglucinol/química , Floroglucinol/farmacologia , Processamento de Proteína Pós-Traducional , Proteoma , Elementos de Resposta , Terpenos/farmacologia , Transfecção
7.
J Agric Food Chem ; 64(21): 4416-25, 2016 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-27157719

RESUMO

Kimchi, a traditional fermented food regularly consumed in Korea, contains various types of antimicrobial compounds. Among the tested compounds present in common spices used in Kimchi, quercetin showed the highest selectivity index against influenza A virus (IAV) H1N1. In this study, the effect of pretreatment and periodic treatment with quercetin against IAV in Madin-Darby canine kidney cells was observed. Compared to pretreatment, periodic treatment resulted in significantly higher cell viability but lower relative expression of the IAV PA gene and total apoptosis and cell death. To explain the mechanisms underlying the antiviral effects of quercetin treatment, a comparative proteomic analysis was performed in four samples (mock, quercetin-treated, IAV-infected, and quercetin-treated IAV-infected). Among the 220 proteins, 56 proteins were classified nonhierarchically into three clusters and were differentially modulated by quercetin treatment in IAV-infected cells. Post-translational modifications were identified in 68 proteins. In conclusion, periodic treatment with quercetin is effective in reducing IAV infection, and differentially regulates the expression of key proteins, including heat shock proteins, fibronectin 1, and prohibitin to reduce IAV replication.


Assuntos
Antivirais/farmacologia , Vírus da Influenza A Subtipo H1N1/efeitos dos fármacos , Influenza Humana/genética , Proteínas/genética , Quercetina/farmacologia , Animais , Linhagem Celular , Cães , Humanos , Vírus da Influenza A Subtipo H1N1/genética , Vírus da Influenza A Subtipo H1N1/fisiologia , Influenza Humana/metabolismo , Influenza Humana/virologia , Proteínas/metabolismo , Proteômica , Replicação Viral/efeitos dos fármacos
8.
J Food Prot ; 78(5): 1046-51, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-25951406

RESUMO

Outbreaks of hepatitis A virus (HAV) infections are most frequently associated with the consumption of contaminated oysters. A rapid and selective concentration method is necessary for the recovery of HAV from contaminated oysters prior to detection using PCR. In this study, ricin extracted from castor beans (Ricinus communis) was tested as an alternative to antibody used in immunomagnetic separation while concentrating HAV prior to its detection using reverse transcription PCR. Initially, the extracted proteins from castor beans were fractionated into 13 fractions by gel filtration chromatography. Pretreatment of different protein fractions showed a variation in binding of HAV viral protein (VP) 1 to oyster digestive tissue in the range of 25.9 to 63.9%. The protein fraction, which caused the highest reduction in binding of VP1 to the tissue, was identified as ricin A by quadrupole time-of-flight mass spectrometry. Ricin A could significantly inhibit binding of VP1 to the tissue with a 50% inhibitory concentration of 4.5 µg/ml and a maximal inhibitory concentration of 105.2%. The result showed that the rate of inhibition of HAV binding to tissue was higher compared to the rate of ricin itself binding to HAV (slope: 0.0029 versus 0.00059). However, ricin A concentration showed a higher correlation to the relative binding of ricin itself to HAV than the inhibition of binding of HAV to the tissue (coefficient of determination, R(2): 0.9739 versus 0.6804). In conclusion, ricin A-linked magnetic bead separation combined with reverse transcription PCR can successfully detect HAV in artificially seeded oyster digestive tissue up to a 10(-4) dilution of the virus stock (titer: 10(4) 50% tissue culture infective dose per ml).


Assuntos
Vírus da Hepatite A/isolamento & purificação , Ostreidae/virologia , Ricina/química , Alimentos Marinhos/virologia , Frutos do Mar/virologia , Animais , Semente de Rícino/química , Contaminação de Alimentos/análise , Microbiologia de Alimentos , Separação Imunomagnética , Ligação Proteica , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Proteínas Virais/genética , Proteínas Virais/metabolismo
9.
Fish Shellfish Immunol ; 45(1): 184-93, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25862970

RESUMO

The effect of curcumin pretreatment (15-240 µM) in fathead minnow cells infected with viral hemorrhagic septicemia virus (VHSV) was evaluated. Cell viability, apoptosis and viral copy number were analyzed using Cell Counting Kit-8 assay, Annexin V staining, and reverse transcription-PCR, respectively. Pretreatment with 120 µM curcumin showed an increase in viability (>90% of mock) of VHSV-infected cells and reduction in the copy number (0.2-log reduction in VHSV N gene expression), reactive oxygen species and apoptosis in the cells without cytotoxic effects. To understand the mechanisms underlaying the antiviral effects of curcumin pretreatment, a comparative proteomic analysis was performed in four samples (M, mock; C, curcumin-treated; V, VHSV-infected; and CV, curcumin-treated VHSV-infected) in triplicate. In total, 185 proteins were detected. The analysis showed that three proteins, including heat shock cognate 71 (HSC71), actin, alpha cardiac muscle (ACTC1) and elongation factor 1 (EEF1) were differentially expressed between V and CV samples. Network analysis performed by Ingenuity Pathways Analysis (IPA) showed that HSC71 was the primary protein interacting with fibronectin (FN) 1, actins (ACTB, ACTG, F-actin) and gelsolin (GSN) in both V and CV samples and thus is a strong target candidate for the protection from VHSV infection at the viral entry stage. Our proteomics data suggest that curcumin pretreatment inhibits entry of VHSV in cells by downregulating FN1 or upregulating F-actin. For both proteins, HSC71 acts as a binding protein that modulates their functions. Furthermore, consistent with the effect of a heat shock protein inhibitor (KNK437), curcumin downregulated HSC71 expression with increasing viability of VHSV-infected cells and inhibited VHSV replication, suggesting that the downregulation of HSC71 could be responsible for the antiviral activity of curcumin. In conclusion, this study indicates that the suppression of viral entry by rearrangement of the F-actin/G-actin ratio via downregulating HSC71 is a plausible mechanism by which curcumin pretreatment controls the early stages of VHSV infection.


Assuntos
Antivirais/farmacologia , Curcumina/farmacologia , Cyprinidae , Doenças dos Peixes/virologia , Septicemia Hemorrágica Viral/virologia , Novirhabdovirus/efeitos dos fármacos , Animais , Antivirais/administração & dosagem , Curcumina/administração & dosagem , Expressão Gênica/efeitos dos fármacos , Novirhabdovirus/fisiologia , Proteínas Virais/genética , Proteínas Virais/metabolismo , Replicação Viral/efeitos dos fármacos
10.
Fish Shellfish Immunol ; 39(2): 464-74, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-24931624

RESUMO

Lectins found in fish tissues play an important role in the innate immune response against viral infection. A fucose-binding type lectin, RbFTL-3, from rock bream (Oplegnathus fasciatus) was identified using expressed sequence tag (EST) analysis. The expression of RbFTL-3 mRNA was higher in intestine than other tissues of rock bream. To determine the function of RbFTL-3, VHSV-susceptible fathead minnow (FHM) cells were transfected with pcDNA3.1(+) or pcDNA3.1(+)-RbFTL-3 and further infected with VHSV. The results show that the viability of FHM cells transfected with pcDNA3.1(+)-RbFTL-3 is higher than that of cells transfected with pcDNA3.1(+) (relative cell viability: 28.9% vs 56.2%). A comparative proteomic analysis, performed to explore the proteins related to the protective effect of RbFTL-3 in the cells during VHSV infection, identified 90 proteins differentially expressed in VHSV-infected FHM cells transfected with pcDNA3.1(+) or pcDNA3.1(+)-RbFTL-3. The expression of RbFTL-3 inhibits a vascular-sorting protein (SNF8) and diminishes the loss of prothrombin, which are closely associated with controlling viral budding and hemorrhage in fish cells, respectively. Subsequent Ingenuity Pathways Analysis enabled prediction of their biofunctional groupings and interaction networks. The results suggest RbFTL-3 modulates the expression of proteins related to viral budding (SNF8, CCT5 and TUBB) and thrombin signaling (F2) to increase the viability of VHSV infected cells.


Assuntos
Cyprinidae , Doenças dos Peixes/imunologia , Doenças dos Peixes/virologia , Lectinas/metabolismo , Novirhabdovirus , Proteoma/metabolismo , Infecções por Rhabdoviridae/veterinária , Animais , Sobrevivência Celular/efeitos dos fármacos , Cromatografia Líquida de Alta Pressão , Biologia Computacional , Primers do DNA/genética , Complexos Endossomais de Distribuição Requeridos para Transporte/antagonistas & inibidores , Etiquetas de Sequências Expressas , Lectinas/farmacologia , Espectrometria de Massas , Protrombina/metabolismo , Infecções por Rhabdoviridae/imunologia , Transfecção
11.
Viruses ; 6(3): 1037-48, 2014 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-24599279

RESUMO

The accuracy and sensitivity of PCR-based methods for detection of hepatitis A virus (HAV) are dependent on the methods used to separate and concentrate the HAV from the infected cells. The pH and ionic strength affect the binding affinity of the virus to cells. In this study, we initially investigated the effects of pH (4.0-10.0) and metal ions (Fe²âº, Co²âº, Cu²âº, Mg²âº, K⁺, and Ca²âº) on the binding of HAV to oyster digestive cells. The lowest relative binding (RB) of HAV to the cells was found at pH 4.0 and in FeSO4 solution (64.6% and 68.1%, respectively). To develop an alternative to antibody-dependent immunomagnetic separation prior to detection of HAV using RT-PCR, the binding of HAV to five lectins, peanut agglutinin (PNA), Dolichos biflorus agglutinin (DBA), Helix pomatia agglutinin (HPA), Ulex europaeus agglutinin (UEA-1) and soybean agglutinin (SBA), was evaluated using ELISAs. SBA showed significantly higher RB to HAV than the other lectins tested. In addition, HAV could be concentrated within 30 min using SBA-linked magnetic bead separation (SMS) prior to the RT-PCR assay. Our findings demonstrate the feasibility of using SMS combined with RT-PCR to detect HAV at dilutions ranging from 10⁻¹-10⁻4 of a HAV stock (titer: 104 TCID50/mL).


Assuntos
Vírus da Hepatite A/isolamento & purificação , Hepatite A/diagnóstico , Separação Imunomagnética/métodos , Lectinas/metabolismo , Manejo de Espécimes/métodos , Humanos , Concentração de Íons de Hidrogênio , Metais/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Fatores de Tempo , Ligação Viral
12.
J Food Prot ; 76(4): 707-11, 2013 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-23575139

RESUMO

Current molecular methods that include PCR have been used to detect norovirus in many food samples. However, the protocols require removing PCR inhibitors and incorporate time-consuming concentration steps to separate virus from analyte for rapid and sensitive detection of norovirus. We developed an immunomagnetic separation (IMS) and a quantum dots (QDs) assay to detect norovirus eluted from fresh lettuce with Tris buffer containing 1% beef extract (pH 9.5). IMS facilitated viral precipitation with a 10-min incubation, whereas virus concentration using polyethylene glycol (PEG) requires more than 3 h and an additional high-speed centrifugation step to precipitate virus before reverse transcription PCR (RT-PCR) analysis. The fluorescence intensity of QDs was detected qualitatively on norovirus dilutions of 10(-1) to 10(-3) in a stool suspension (100 RT-PCR units/ml). The results suggest that a fluorescence assay based on IMS and QDs is valid for detecting norovirus qualitatively according to fluorescent signal intensity within the same virus detection limit produced by IMS-RT-PCR and PEG-RT-PCR.


Assuntos
Contaminação de Alimentos/análise , Separação Imunomagnética/métodos , Alface/virologia , Norovirus/isolamento & purificação , Pontos Quânticos , Contagem de Colônia Microbiana , Qualidade de Produtos para o Consumidor , Microbiologia de Alimentos , Humanos , Sensibilidade e Especificidade , Fatores de Tempo
13.
Exp Mol Med ; 41(11): 772-81, 2009 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-19641380

RESUMO

Long-lived people may have a unique genetic makeup that makes them more resistant than the general population to prevalent age-related diseases; however, not much is known about genes involved in the longevity. To identify susceptibility variants controlling longevity, we performed a high-throughput candidate gene study using 137 Koreans over 90 yr old and 213 young healthy Koreans. We evaluated 463 informative markers located in 176 candidate genes mostly for diabetes mellitus, cardiovascular disease and cancer under five genetic models. We estimated the odds ratios for each allele, genotype, haplotype, and gene-gene interaction using logistic regression analysis. Associations between 13 genes and longevity were detected at a P-value less than 0.01. Particularly, the rs671 (A) allele of the aldehyde dehydrogenase 2 family (mitochondrial) (ALDH2) gene was associated with longevity only in men (OR 2.11, P =0.008). Four genes, proprotein convertase subtilisin/kexin type 1 (PCSK1, P=0.008), epidermal growth factor receptor (EGFR, P=0.003), paired box 4 (PAX4, P=0.008), and V-yes-1 Yamaguchi sarcoma viral related oncogene homolog (LYN, P=0.002) consistently yielded statistical evidence for association with longevity. The findings of the current study may provide a starting point for future studies to unravel genetic factors controlling longevity in Koreans.


Assuntos
Doenças Cardiovasculares/genética , Diabetes Mellitus/genética , Longevidade/genética , Neoplasias/genética , Polimorfismo Genético , Adulto , Idoso , Idoso de 80 Anos ou mais , Aldeído Desidrogenase/genética , Aldeído-Desidrogenase Mitocondrial , Alelos , Grupo com Ancestrais do Continente Asiático/etnologia , Grupo com Ancestrais do Continente Asiático/genética , Doenças Cardiovasculares/etnologia , Diabetes Mellitus/etnologia , Receptores ErbB/genética , Feminino , Marcadores Genéticos/genética , Haplótipos , Proteínas de Homeodomínio/genética , Humanos , Coreia (Geográfico) , Masculino , Pessoa de Meia-Idade , Neoplasias/etnologia , Fatores de Transcrição Box Pareados/genética , Pró-Proteína Convertase 1/genética , Fatores Sexuais , Quinases da Família src/genética
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