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1.
Medicine (Baltimore) ; 100(32): e26880, 2021 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-34397904

RESUMO

ABSTRACT: Poor oral hygiene can be potentially life-threatening in inpatients. However, no basic protocol on oral hygiene customized for inpatients exists, and lack of oral care related knowledge, attitude, and skills among caregivers could be detrimental to the general health of patients. This study sought to identify the oral care practices and oral health status of inpatients with varying physical activity limitations in a rehabilitation ward.Sixty-one inpatients in a rehabilitation ward were evaluated for their medical and physical conditions and oral health status. These were assessed using the bedside oral exam, decayed, missing, filled teeth index, plaque index, gingival index, and caries activity test.In total, 40 men and 21 women (mean age, 56.6 years) were included in this study. Among them, 50.8% of the patients could brush their teeth unassisted, whereas 49.2% required assistance from an assistant for oral care. The proportion of patients receiving nasogastric tube feeding was higher in the group that could not provide oral self-care; 36.7% and 33.3% of these patients showed moderate and severe dysfunction, respectively, based on bedside oral exam. Scores for the swallowing, tongue, and total domains of bedside oral exam were poorer for patients who could not provide oral self-care (P < .01). The caries activity test indicated a moderate risk for both groups.Our findings suggest that an oral care protocol that considers the physical activity limitations in inpatients in rehabilitation wards is necessary to minimize negative influences on the systemic health of these patients.


Assuntos
Atividades Cotidianas , Cárie Dentária , Saúde Bucal/normas , Higiene Bucal , Autocuidado , Cuidadores/educação , Cárie Dentária/diagnóstico , Cárie Dentária/prevenção & controle , Feminino , Conhecimentos, Atitudes e Prática em Saúde , Humanos , Pacientes Internados/estatística & dados numéricos , Masculino , Pessoa de Meia-Idade , Determinação de Necessidades de Cuidados de Saúde , Higiene Bucal/educação , Higiene Bucal/métodos , Índice Periodontal , Desempenho Físico Funcional , Centros de Reabilitação/estatística & dados numéricos , República da Coreia/epidemiologia , Autocuidado/métodos , Autocuidado/estatística & dados numéricos
2.
BMC Health Serv Res ; 21(1): 748, 2021 Jul 28.
Artigo em Inglês | MEDLINE | ID: mdl-34315462

RESUMO

BACKGROUND: Professional oral care in the intensive care unit may reduce the incidence of Ventilator Associated Pneumonia, which increases the patient's mortality rate. This study aimed to develop a competency for professional oral hygiene care of endotracheally-intubated intensive-care patients. METHODS: First, we developed a competency draft by reviewing the literature on oral hygiene care of patients in the intensive care unit. Next, we developed expert validity test questionnaires using this draft and conducted expert validity tests twice on 18 experts. We determined competency as a content validity index of 0.8 or more and received expert additive opinions about competency through an open-questionnaire expert validity test paper in this methodology study. RESULTS: The content validity index ranged from 0.8 ~ 1.0 for all items. The competency of 'professionalism' comprised 2 sub-competencies with 7 behavioral indicators. 'POHC preparation' comprised 3 sub-competencies with 10 behavioral indicators. 'POHC implementation' comprised 3 sub-competencies with 6 behavioral indicators. 'POHC evaluation' comprised 2 sub-competencies with 8 behavioral indicators. Lastly 'Cooperation among experts' comprised 3 sub-competencies with 7 behavioral indicatiors. CONCLUSIONS: To provide patients with high quality oral hygiene care, these competencies should be implemented, and oral hygiene care professionals and related medical personnel should form a cooperative system.


Assuntos
Unidades de Terapia Intensiva , Higiene Bucal , Cuidados Críticos , Humanos , Profissionalismo , Inquéritos e Questionários
3.
Int J Chron Obstruct Pulmon Dis ; 16: 1599-1606, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34113092

RESUMO

Purpose: Up to 20% of patients with chronic obstructive pulmonary disease (COPD) require re-admission within 30 days of discharge after hospitalization for acute exacerbations of the disease. These re-admissions can increase morbidity and the economic burden of COPD. Reducing re-admissions has become a policy target in many developed countries. We investigated the risk factors for COPD re-admissions among older adults with COPD. Patients and Methods: Data obtained from the National Health Insurance Service-Senior Cohort (NHIS-SC) in Korea were analyzed. The subjects included 558,147 patients aged ≥70 who had been admitted for COPD between 2013 and 2015. Re-admission was defined as being re-hospitalized within 30 days after discharge. The key variables selected from the database included income-based insurance contributions, demographical variables, information on inpatient medical services, types of healthcare facilities, and emergency time relevance index (TRI). The TRI is a regional medical-use analysis index that evaluates whether the capacity of the medical services available is appropriate for the medical needs of the target residents. Results: In 814 COPD re-admission cases among 4867 total admissions due to COPD in elderly subjects, higher re-admission rates were associated with male sex, admission to district hospitals, medical aid recipients, and a longer hospital stay. When additionally adjusting the TRI to identify the difference in re-admission rates due to medical service accessibility, the same results were found, except for the areas of residence. The TRI was lower in re-admission cases (odds ratio 0.991 [95% CI, 0.984‒0.998], P = 0.013). Conclusion: In this study, COPD re-admission rates among older adults were significantly associated with sex, length of hospital stay, and the type of hospital. The capacity of the medical services provided was also related to the COPD re-admission rate. Better access to appropriate emergency services is associated with reduction of COPD re-admission rates.


Assuntos
Readmissão do Paciente , Doença Pulmonar Obstrutiva Crônica , Idoso , Hospitalização , Humanos , Pacientes Internados , Tempo de Internação , Masculino , Doença Pulmonar Obstrutiva Crônica/diagnóstico , Doença Pulmonar Obstrutiva Crônica/epidemiologia , Doença Pulmonar Obstrutiva Crônica/terapia , Estudos Retrospectivos
4.
BMC Oral Health ; 21(1): 233, 2021 05 04.
Artigo em Inglês | MEDLINE | ID: mdl-33941148

RESUMO

BACKGROUND: This cross-sectional study aimed to examine the relationship between sleep habits and oral disease symptoms in adolescents. METHODS: Among 62,276 adolescents who participated in the 13th Korea Youth Risk Behavior Web-based Survey (2017), we selected a total of 54,766 adolescents (age, 12-18 years; male, 49.9%) for the final analysis, after excluding those who did not report their sleep duration. The 13th Korea Youth Risk Behavior Web-based Survey data were obtained from a stratified, multistage, clustered sample. Independent variables included general characteristics, oral health behavior, sleep types, sleep duration, and sleep quality; dependent variables comprised oral disease symptoms. Sleep was categorized according to bedtime astype A (bedtime < 1 a.m.) and type B (bedtime ≥ 1 a.m.). Data were analyzed using logistic regression analysis. Statistical significance was set at p < 0.05. RESULTS: After adjusting for all covariates, adolescents with type A sleep had a higher risk of toothache on chewing (OR = 1.08, 95% CI 1.02-1.15) than adolescents with type B. Adolescents who slept for 6 h or less each night had a higher risk of pain in the tongue and buccal mucosa (OR = 1.35, 95% CI 1.18-1.54), gingival pain, and bleeding (OR = 1.31, 95% CI 1.19-1.45) than those who slept for more than 8 h. Adolescents with low quality of sleep had a higher risk of toothache or throbbing (OR = 1.70, 95% CI 1.60-1.81), toothache on chewing (OR = 1.73, 95% CI 1.65-1.82), and halitosis (OR = 1.51, 95% CI 1.41-1.59) than those with high quality of sleep. CONCLUSIONS: Our findings indicate that some oral symptoms are related to sleep duration and quality. It is essential to inculcate good sleeping habits in adolescents by emphasizing the effects of inadequate sleep duration and quality.


Assuntos
Assunção de Riscos , Sono , Adolescente , Criança , Estudos Transversais , Humanos , Internet , Masculino , República da Coreia/epidemiologia , Inquéritos e Questionários
5.
Int J Dent Hyg ; 18(3): 307-313, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32196912

RESUMO

OBJECTIVE: High stress levels experienced by medical professionals are a well-established phenomenon in current literature. However, there are few studies focusing on stress experienced in the field of oral health or on the sense of coherence (SOC) that helps to actively respond to job stress. The purpose of this study was to analyse if there is an association between SOC and job stress among dental hygienists in Korea. METHODS: A cross-sectional study was conducted on a convenience sample of 441 dental hygienists in the Seoul Gyeonggi province, Korea. The independent variable was SOC which had three dimensions: comprehensibility, manageability and meaningfulness. The dependent variable was job stress, which was evaluated using the Korean Occupational Stress Scale Short Form. Confounding factors were age, marital status, educational background, type of hospital, and work experience. The chi-square test and t test measures were used for bivariate analysis. Multiple logistic regression analysis was performed to confirm the association between SOC and job stress. The collected data were statistically analysed using SPSS version 22.0. RESULTS: The SOC score showed a significant difference in relation to the job stress score. The SOC score was high when the job stress was low (P < .001). Overall SOC scores showed an inverse correlation with job stress. CONCLUSION: This study reports that a higher SOC is associated with lower job stress in Korean dental hygienists. Since a higher SOC in dental hygienists indicated that they could cope with job stress more positively, it is important to increase their SOC.


Assuntos
Estresse Ocupacional , Senso de Coerência , Estudos Transversais , Higienistas Dentários , Humanos , República da Coreia , Inquéritos e Questionários
6.
Photodiagnosis Photodyn Ther ; 28: 277-281, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31600575

RESUMO

BACKGROUND: To utilize plaque scoring indices as objective measures, a certain standard of intra-rater and inter-rater agreement must be met. Achievement of high consistency among several examiners in the assessment of images from Q-ray cam (for quantitative light-induced fluorescence-digital imaging) and digital single-lens reflex camera of stained tooth surfaces might enable more efficient undertaking of large epidemiological studies and oral tests. METHODS: Second-year dental hygiene students and registered dental hygienists 40 examiners were randomly selected, all of whom provided informed consent. Multiple examiners assessed plaque scoring indices from the two types of images of the same 30 patients. The images were observed on a computer monitor, and the area with dental plaque was assessed using the Patient Hygiene Performance Index. The intra-class correlation coefficient was determined to evaluate the agreement among examiners and test methods for dental plaque Q-ray cam and digital single-lens reflex camera measurements were compared using a Bland-Altman plot. The intra-class correlation coefficient was analyzed using SPSS version 24 software (SPSS Inc., Chicago, IL, USA), and the exploratory graph was analyzed with MedCalc. RESULTS: Agreement among 40 examiners was excellent with an intra-class correlation coefficient of 0.990 when the Q-ray cam was used. The intra-class correlation coefficient for the registered dental hygienists group was 0.987 and that for the dental hygiene students group was 0.980. CONCLUSIONS: The study was high agreement in the assessment of Q-ray cam images and digital single-lens reflex camera images of stained dental plaque by multiple examiners, confirming that the two methods are validated both independently and with high mutual agreement.


Assuntos
Corantes/administração & dosagem , Índice de Placa Dentária , Imagem Óptica/instrumentação , Educação em Odontologia , Humanos , Variações Dependentes do Observador , Fotografação
7.
Neurobiol Dis ; 132: 104538, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31344491

RESUMO

It is challenging to revitalize ischemic penumbra after an acute stroke with intracranial perfusion insufficiency. To evaluate whether cranial burr hole and erythropoietin (EPO) generate effective revascularization, we investigated the efficacy of the augmentation method for reverse arteriogenesis from the healthy extracranial milieu. An intracranial perfusion insufficiency was created through bilateral internal carotid artery ligation (bICAL) in Sprague-Dawley rats. We administered recombinant human EPO (5000 U/kg) or saline intraperitoneally for 3 days after bICAL. Mechanical barrier disruption (MBD) was performed through a cranial burr hole with small dural cracks in the right hemisphere. The ipsilateral hemisphere with MBD grossly showed vascular networks between the extra- and intra-cranial spaces 2 weeks after the MBD procedure. It also showed significantly increased vessels in the intracranial vasculature adjacent to the MBD region (p = 0.0006). The levels of pro-angiogenic and inflammatory factors with prominent markers of vessel permeability were also significantly increased (MBD-only vs. control; Tnf-α, p = 0.0007; Vegf, p = 0.0206). In the EPO-administered group, such elevations in inflammation were significantly mitigated (combined vs. MBD-only; Tnf-α, p = 0.0008). The ipsilateral hemisphere with MBD-EPO (vs. MBD-only) showed significantly increased vessels (RECA-1, p = 0.0182) and their maturation (RECA-1/α-SMA, p = 0.0046), with upregulation of tumor growth factor-ß1 (Tgf-ß1, p = 0.037) and matrix metalloproteinase-2 (Mmp-2, p = 0.0488). These findings were completely blocked by minocycline (MIC) administration during in vivo (Tgf-ß1, p = 0.0009; Mmp-2, p < 0.0001) and in vitro experiments (tube formation, p < 0.0001). Our data suggest that the MBD procedure (for angiogenic routes) and EPO administration (for an arteriogenic booster) are complimentary and can facilitate successfully "reverse arteriogenesis" in subjects with intracranial perfusion insufficiency.


Assuntos
Artérias Cerebrais/diagnóstico por imagem , Revascularização Cerebral/métodos , Craniotomia/métodos , Eritropoetina/administração & dosagem , Doença de Moyamoya/diagnóstico por imagem , Doença de Moyamoya/tratamento farmacológico , Adulto , Idoso , Animais , Angiografia Cerebral/métodos , Artérias Cerebrais/efeitos dos fármacos , Terapia Combinada/métodos , Células Endoteliais da Veia Umbilical Humana , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Estudos Retrospectivos , Crânio/irrigação sanguínea , Crânio/diagnóstico por imagem , Crânio/efeitos dos fármacos
8.
Int Dent J ; 69(5): 376-382, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31077367

RESUMO

INTRODUCTION: The purpose of the present study was to investigate current sugar-sweetened beverage consumption habits in Korean adolescents in conjunction with their demo-socioeconomic characteristics, and to identify variables that affect such behaviours. MATERIALS AND METHODS: The study was conducted via secondary analysis of data from the 2015 Youth Risk Behavior Web-Based Survey (the 11th survey). The dependent variable in the current study was the combined consumption frequency of three types of sugar-sweetened beverages (carbonated beverages, highly-caffeinated beverages, sugary beverages), and demographic (gender, academic grade, residence), sociological (father's education level, mother's education level, having experienced depression within the last 12 months) and economic (subjective economic status, weekly allowance) factors constituted the independent variables. RESULTS: Our results showed that the amount of weekly allowance had the greatest impact on adolescents' beverage consumption of more than seven times a week. Increases in experiencing a depressed mood and allowance were associated with an increased tendency to consume ≥ 7 sugar-sweetened beverages per week. CONCLUSION: The results of the current study suggest that families and society should work collectively to motivate adolescents to consciously choose and buy healthy snacks. Furthermore, society should reach a consensus and invest effort to resolve this issue continuously and gradually, such as by presenting a standard for consumption of sugar-added drinks and implementing regulations to ban sales to adolescents.


Assuntos
Sacarose na Dieta , Açúcares , Adolescente , Bebidas , Humanos , Internet , República da Coreia , Assunção de Riscos , Inquéritos e Questionários
9.
Am J Orthod Dentofacial Orthop ; 155(3): 355-361, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30826038

RESUMO

INTRODUCTION: This study aimed to evaluate the associations between normative orthodontic treatment need and oral health-related quality of life (OHRQoL) at an item level as well as subscale and total score levels among schoolchildren. METHODS: A cross-sectional study was conducted on 2,010 randomly selected children aged approximately 8, 10, 12, and 15 years residing in the Gyeonggi province, Korea. Children were clinically examined with the Index of Orthodontic Treatment Need (IOTN). To measure OHRQoL, the Child Oral Health Impact Profile (COHIP) was used. RESULTS: In the physical health domain of COHIP, crooked teeth, discoloration, "food sticking," and "difficulty keeping teeth clean" were significantly associated with IOTN levels (P < 0.05), whereas pain, sensitivity and bleeding, bad breath, mouth breathing, and dry mouth were not. In addition, present and future dental health were significantly associated with IOTN levels, although self-confidence, attractiveness, and future health were not. As expected, the 5 subscale scores and total score of COHIP were significantly associated with IOTN levels after adjustment for gender, age, socioeconomic level, and caries state. CONCLUSIONS: This study suggests the importance of in-depth investigation at the item level of OHRQoL assessment scales to better evaluate the impact of malocclusion that might be alleviated by orthodontic treatment.


Assuntos
Índice de Necessidade de Tratamento Ortodôntico , Saúde Bucal , Qualidade de Vida , Adolescente , Criança , Estudos Transversais , Feminino , Humanos , Masculino , República da Coreia , Fatores Socioeconômicos
10.
J Occup Health ; 61(4): 297-304, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30839156

RESUMO

OBJECTIVES: In dental clinics, dental hygienists are exposed to aerosolized pathologic bacteria, which can be transmitted to the oral cavity via lip cosmetics. Accordingly, such contamination poses a consistent health risk among staffs. Our study examined the bacterial contamination of lip cosmetics used by dental hygienists while in a clinic setting. METHODS: Sixteen dental hygienists were surveyed regarding their job assignments and habits associated with lip cosmetic. Subsequently, microorganisms were analyzed in collected samples of the hygienists' lip cosmetics using colony-forming unit (CFU) assays, 16s-rDNA polymerase chain reaction, and DNA sequencing. RESULTS: Notably, 81.3% of the submitted lip cosmetic samples were contaminated, with bacterial CFUs ranging from undetectable to innumerable. Many samples (43.8%) exceeded the microbial limits of cosmetic contamination. Of the lip cosmetic used for more than 6 months, 60% exceeded the microbial limit. When wearing a mask every time, only one of the six samples exceeded the microbial limit. More frequent dental mask changing was associated with a lower likelihood that the cosmetic sample would exceed the microbial limit. No samples from hygienists who changed their masks four times a day exceeded the microbial limit, compared to 33.3% from hygienists who only changed the mask when it became wet. Most isolated bacteria were gram-positive, facultative anaerobic, asporogenic, and opportunistically pathogenic, and the most prevalent species were Staphylococcus aureus, Streptococcus salivarius, and Staphylococcus epidermidis. CONCLUSION: Our findings indicate that dental staff, including dental hygienists, should exercise more careful workplace habits, particularly with regard to infection control and cosmetic use.


Assuntos
Bactérias/isolamento & purificação , Cosméticos , Higienistas Dentários , Lábio/microbiologia , Exposição Ocupacional , Adulto , Microbiologia do Ar , Estudos Transversais , Feminino , Humanos , Projetos Piloto , Dispositivos de Proteção Respiratória , Inquéritos e Questionários , Adulto Jovem
11.
Int J Dent Hyg ; 17(2): 177-182, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30485645

RESUMO

OBJECTIVES: The purpose of the present study was to analyse the effects of working posture, physical balance and work accumulation on shifts in plantar pressure in dental hygienists, by measuring REBA, which evaluates working posture, and plantar pressure, which evaluates physical balance. METHODS: This study was conducted on 24 dental hygienists currently working in dental clinics. The ergonomic assessment method of the Rapid Entire Body Assessment was used to evaluate working posture in the dental hygienists, and a Gait Analyzer was used to measure plantar pressure. RESULTS: The subjects' mean REBA score was 4.96 ± 1.04 points, and 87.5% of the subjects showed poor working posture, with a REBA score of at least four points. Among subjects with a REBA score of four points or more, seven of the eight parts of the sole of the foot showed significant differences in plantar pressure between the right and left feet (P < 0.05). When we examined changes in plantar pressure over a week, pressure increased on the left side, and in particular, for subjects with a REBA score of four points or higher, pressure increased in the left foot and in the anterior right foot. CONCLUSIONS: Most of the dental hygienists in this study showed poor posture during working. Subjects with poor posture at a level requiring intervention showed differences in plantar pressure between the right and left feet. Inappropriate posture can cause musculoskeletal disorders. Therefore, it will be necessary to prepare internal and external measures to maintain proper working posture in dental hygienists, including education, exercise, improvement of the working environment and improvement of ergonomic equipment and devices.


Assuntos
Higienistas Dentários , Ergonomia/métodos , Pé/fisiologia , Pressão , Trabalho/fisiologia , Adulto , Meio Ambiente , Ergonomia/instrumentação , Exercício Físico , Feminino , Educação em Saúde , Humanos , Masculino , Doenças Musculoesqueléticas/etiologia , Doenças Musculoesqueléticas/prevenção & controle , Doenças Profissionais/etiologia , Doenças Profissionais/prevenção & controle , Equilíbrio Postural , Postura , Jornada de Trabalho em Turnos , Adulto Jovem
12.
Korean J Fam Med ; 39(3): 180-184, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29788707

RESUMO

BACKGROUND: Recently, despite the high prevalence of fatigue in patients, there is a lack of research on the quality of life (QoL) in unexplained fatigue patients, indicating that they are not properly diagnosed and treated. The aim of this study was to compare fatigue severity and QoL between patients with explained and unexplained fatigue. METHODS: The study consisted of 200 Korean adults who complained of fatigue without underlying disease. Fatigue Severity Scale, Short Form Health Survey-36 version 2 (SF-36v2), and Beck Depression Inventory-II (BDI-II) self-questionnaires were administered. Participants were dichotomized to two groups, namely, patients with unexplained or explained fatigue, sorted according to laboratory examination results. The chi-square test, t-test, and Wilcoxon rank-sum test were used, and analysis of covariance was calculated after adjusting for age, sex, body mass index, smoking status, and physical component summary (PCS) of SF-36v2 or BDI-II. RESULTS: PCS of SF-36v2 between the two groups showed significant difference. Compared to patients with explained fatigue, those with unexplained fatigue showed lower physical component scores of QoL. CONCLUSION: QoL of patients with unexplained fatigue could largely diminish than those with explained fatigue. The primary clinician should be aware of poor QoL in patients with unexplained fatigue to identify who is in need of more attention and intervention.

13.
Maturitas ; 107: 68-70, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29169583

RESUMO

We conducted a study on the relationship between leukocyte telomere length (LTL) and quality of life (QoL) in 82 couples aged 55 and older. LTL was measured by quantitative real-time PCR. QoL was evaluated using the physical component score (PCS) and mental component score (MCS) on the Korean version of the Short-Form Health Survey (SF-36). LTL was found to be independently associated with the SF-36 PCS in males (ß=0.014, p=0.03) and the SF-36 MCS in females (ß=1.16, p<0.01). Thus LTL is associated with QoL in gender-specific ways.


Assuntos
Leucócitos , Qualidade de Vida , Telômero , Idoso , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase em Tempo Real
14.
Environ Health Toxicol ; 33(4): e2018017, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30661338

RESUMO

The purpose of this review is to summarize the results of studies on of the association between exposure to dental X-rays and health risk. To perform the systematic review, We searched the PUBMED, EMBASE, and MEDLINE databases for papers published before December 15, 2016. A total of 2 158 studies, excluding duplicate studies, were found. Two reviewers independently evaluated the eligibility of each study. The final 21 studies were selected after application of exclusion criteria. In terms of health outcomes, there were 10 studies about brain tumors, 5 about thyroid cancer, 3 about tumors of head and neck areas, and 3 related to systemic health. In brain tumor studies, the association between dental X-ray exposure and meningioma was statistically significant in 5 of the 7 studies. In 4 of the 5 thyroid-related studies, there was a significant correlation with dental diagnostic X-rays. In studies on head and neck areas, tumors included laryngeal, parotid gland, and salivary gland cancers. There was also a statistically significant correlation between full-mouth X-rays and salivary gland cancer, but not parotid gland cancer. Health outcomes such as leukemia, low birth weight, cataracts, and thumb carcinomas were also reported. In a few studies examining health effects related to dental X-ray exposure, possibly increased risks of meningioma and thyroid cancer were suggested. More studies with a large population and prospective design are needed to elaborate these associations further.

15.
PLoS One ; 12(9): e0185040, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28915276

RESUMO

BACKGROUND AND OBJECTIVE: Skeletal muscle has been suggested as an important factor in the pathophysiology of metabolic syndrome. During the aging process, muscle mass is lost in specific body parts. However, few studies have investigated the relationship between site-specific muscle loss assessed using computed tomography (CT) and metabolic syndrome. This study was conducted to investigate the association between metabolic syndrome and rectus abdominis muscle thickness at the umbilicus level (RAM), which reflects site-specific muscle loss of the abdomen using CT image. METHODS: This cross-sectional study was conducted on 725 middle-aged Korean men. Anthropometric evaluation and biochemical tests were performed. The RAMs of the subjects were measured from CT images taken at the umbilicus level. RESULTS: The mean RAM (mean ±SD) of subjects with metabolic syndrome was 2.46 ±0.01, which was thinner than that of subjects without metabolic syndrome (2.52 ±0.01, p<0.01). Moreover, RAM decreased as the number of metabolic syndrome components increased (p-value for trend<0.01). RAM was positively correlated with body mass index (r = 0.21, p<0.01), skeletal muscle index (r = 0.26, p<0.01), and creatinine (r = 0.12, p<0.01), while RAM was negatively correlated with age(r = -0.11, p<0.01), abdominal circumference(r = -0.22, p<0.01), fasting glucose (r = -0.10, p<0.01), and triglycerides(r = -0.15, p<0.01). Using a stepwise multiple logistic regression analysis, we found that RAM was an independent factor associated with metabolic syndrome (OR: 0.861, 95%CI, 0.779-0.951, p<0.01). The result was not different in the statistical analysis including the components of MS (OR: 0.860, 95% CI, 0.767-0.965, p = 0.01). CONCLUSION: RAM was associated with metabolic syndrome in middle-aged men. Moreover, site-specific muscle loss at the abdomen, as evaluated by RAM, also may be a predictor of metabolic syndrome like SMI.


Assuntos
Síndrome Metabólica , Reto do Abdome , Tomografia Computadorizada por Raios X , Adulto , Grupo com Ancestrais do Continente Asiático , Estudos Transversais , Humanos , Masculino , Síndrome Metabólica/diagnóstico por imagem , Síndrome Metabólica/fisiopatologia , Pessoa de Meia-Idade , Reto do Abdome/diagnóstico por imagem , Reto do Abdome/fisiopatologia , República da Coreia
16.
Sci Rep ; 7: 44323, 2017 03 14.
Artigo em Inglês | MEDLINE | ID: mdl-28290544

RESUMO

Although aerobic CO dehydrogenases (CODHs) might be applicable in various fields, their practical applications have been hampered by low activity and no heterologous expression. We, for the first time, could functionally express recombinant PsCODH in E. coli and obtained a highly concentrated recombinant enzyme using an easy and convenient method. Its electron acceptor spectra, optimum conditions (pH 6.5 and 30 °C), and kinetic parameters (kcat of 12.97 s-1, Km of 0.065 mM, and specific activity of 0.86 Umg-1) were examined. Blast furnace gas (BFG) containing 20% CO, which is a waste gas from the steel-making process, was tested as a substrate for PsCODH. Even with BFG, the recombinant PsCODH retained 88.2% and 108.4% activity compared with those of pure CO and 20% CO, respectively. The results provide not only a promising strategy to utilize CO-containing industrial waste gases as cheap, abundant, and renewable resources but also significant information for further studies about cascade reactions producing value-added chemicals via CO2 as an intermediate produced by a CODH-based CO-utilization system, which would ultimately expand the versatility of CODH.


Assuntos
Aldeído Oxirredutases/metabolismo , Proteínas de Bactérias/metabolismo , Biocombustíveis/análise , Dióxido de Carbono/química , Monóxido de Carbono/química , Complexos Multienzimáticos/metabolismo , Pantoea/química , Aldeído Oxirredutases/genética , Proteínas de Bactérias/genética , Dióxido de Carbono/metabolismo , Monóxido de Carbono/metabolismo , Clonagem Molecular , Escherichia coli/genética , Escherichia coli/metabolismo , Expressão Gênica , Vetores Genéticos/química , Vetores Genéticos/metabolismo , Resíduos Industriais/análise , Cinética , Complexos Multienzimáticos/genética , Pantoea/classificação , Pantoea/enzimologia , Filogenia , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo
17.
Sci Rep ; 6: 39587, 2016 12 22.
Artigo em Inglês | MEDLINE | ID: mdl-28004799

RESUMO

Multistep cascade reactions in nature maximize reaction efficiency by co-assembling related enzymes. Such organization facilitates the processing of intermediates by downstream enzymes. Previously, the studies on multienzyme nanocomplexes assembled on DNA scaffolds demonstrated that closer interenzyme distance enhances the overall reaction efficiency. However, it remains unknown how the active site orientation controlled at nanoscale can have an effect on multienzyme reaction. Here, we show that controlled alignment of active sites promotes the multienzyme reaction efficiency. By genetic incorporation of a non-natural amino acid and two compatible bioorthogonal chemistries, we conjugated mannitol dehydrogenase to formate dehydrogenase with the defined active site arrangement with the residue-level accuracy. The study revealed that the multienzyme complex with the active sites directed towards each other exhibits four-fold higher relative efficiency enhancement in the cascade reaction and produces 60% more D-mannitol than the other complex with active sites directed away from each other.


Assuntos
Complexos Multienzimáticos/química , Nanoestruturas/química , Sítios de Ligação , Domínio Catalítico , DNA/química , Formiato Desidrogenases/química , Cinética , Manitol/química , Manitol Desidrogenases/química , Methanocaldococcus/enzimologia , Oxigênio/química , Probabilidade , Pseudomonas fluorescens/enzimologia , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Thiobacillus/enzimologia
18.
PLoS One ; 11(3): e0152826, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27028506

RESUMO

Multiple-site-specific incorporation of a noncanonical amino acid into a recombinant protein would be a very useful technique to generate multiple chemical handles for bioconjugation and multivalent binding sites for the enhanced interaction. Previously combination of a mutant yeast phenylalanyl-tRNA synthetase variant and the yeast phenylalanyl-tRNA containing the AAA anticodon was used to incorporate a noncanonical amino acid into multiple UUU phenylalanine (Phe) codons in a site-specific manner. However, due to the less selective codon recognition of the AAA anticodon, there was significant misincorporation of a noncanonical amino acid into unwanted UUC Phe codons. To enhance codon selectivity, we explored degenerate leucine (Leu) codons instead of Phe degenerate codons. Combined use of the mutant yeast phenylalanyl-tRNA containing the CAA anticodon and the yPheRS_naph variant allowed incorporation of a phenylalanine analog, 2-naphthylalanine, into murine dihydrofolate reductase in response to multiple UUG Leu codons, but not to other Leu codon sites. Despite the moderate UUG codon occupancy by 2-naphthylalaine, these results successfully demonstrated that the concept of forced ambiguity of the genetic code can be achieved for the Leu codons, available for multiple-site-specific incorporation.


Assuntos
Alanina/análogos & derivados , Códon/metabolismo , Leucina/metabolismo , Naftalenos/metabolismo , Fenilalanina-tRNA Ligase/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo , Saccharomyces cerevisiae/metabolismo , Alanina/genética , Alanina/metabolismo , Anticódon/genética , Anticódon/metabolismo , Códon/genética , Leucina/genética , Fenilalanina-tRNA Ligase/genética , Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/genética
19.
J Atten Disord ; 19(2): 138-46, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-23929521

RESUMO

OBJECTIVE: ADHD is associated with social and emotional impairment that goes beyond the core symptoms of hyperactivity, impulsivity, and attention deficits. This study evaluates the comparative efficacy of emotional management training (EMT) with social skills training (SST) and no treatment in children with ADHD. METHOD: A randomized, controlled treatment outcome study was conducted with 32 boys and 40 girls (aged 10-12 years). The Child Behavior Checklist, Emotion Expression Scale for Children, Child Depression Inventory, and State-Trait Anxiety Inventory for children were completed before and after the intervention. RESULTS: The EMT group exhibited a significant improvement in emotion recognition and expressive reluctance. Therefore, focusing on emotion identification and expression in social cognitive processes (i.e., EMT), instead of merely focusing on social skills (SST), enhances treatment efficacy. CONCLUSION: These results support the hypothesis that focusing on the identification and expression of emotional information processes, instead of merely focusing on social skills (SST) enhances treatment efficacy.


Assuntos
Transtorno do Deficit de Atenção com Hiperatividade/terapia , Terapia Cognitivo-Comportamental/métodos , Emoções , Comportamento Social , Habilidades Sociais , Ansiedade/psicologia , Transtorno do Deficit de Atenção com Hiperatividade/psicologia , Criança , Depressão/psicologia , Inteligência Emocional , Emoções Manifestas , Feminino , Humanos , Masculino , Escalas de Graduação Psiquiátrica , Reconhecimento Psicológico , Percepção Social , Inquéritos e Questionários , Resultado do Tratamento
20.
Biochem Biophys Res Commun ; 419(4): 676-81, 2012 Mar 23.
Artigo em Inglês | MEDLINE | ID: mdl-22382031

RESUMO

GFP and its derivatives are commonly used as non-invasive in vivo reporters. These fluorescent proteins have been employed to analyze expression level, localization and movement of proteins, as well as protein interaction. However, they cannot be utilized under anaerobic conditions due to the oxygen requirement for the maturation of the fluorophore. Thus, other proteins with a different mechanism of fluorescence emission are needed. We reported here a blue fluorescent protein, named mBFP, that was derived from metagenomic DNA. This protein consisting of 248 amino acids was overexpressed (>35% of the total protein) in a soluble form in Escherichia coli. mBFP showed a distinct fluorescence pattern that was NADPH-dependent and could be used to image live cells under anaerobic conditions. Thus, mBFP holds great promise for use as a reporter in a broad range of applications.


Assuntos
Fungos/genética , Genes Reporter , Proteínas Luminescentes/química , Proteínas Luminescentes/genética , Metagenoma , Oxigênio/química , Folhas de Planta/microbiologia , Sequência de Aminoácidos , Clonagem Molecular , Escherichia coli/genética , Biblioteca Gênica , Vetores Genéticos/genética , Proteínas Luminescentes/isolamento & purificação , Dados de Sequência Molecular , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/isolamento & purificação , Seleção Genética , Solubilidade
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