Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 8 de 8
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Biosens Bioelectron ; 91: 424-430, 2017 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-28064127

RESUMO

A novel thionine unites with platinum nanowire inlaid globular SBA-15 (Pt NWs@g-SBA-15/Thi) not only utilizes as an efficient electrical signal probe but also constitutes an amplifying system with double-deck gold film (D-Au film) have been applied to the fabrication of sandwich-type immunosensor for detecting hepatitis B surface antigen (HBs Ag). The D-Au film can accelerate the electron transfer on the electrode interface due to the tunneling effect between the two Au films and can improve the load capacity of primary antibodies (Ab1) because of the good biocompatibility. The Pt NWs@g-SBA-15/Thi with uniform globular morphology not only can effectively reduce the spatial limitation for loading the secondary antibodies (Ab2) but also can provide outstanding pore accessibility of guest species from outside and offer catalytically active sites in a large scale. Besides, the presence of Thi can well enhance the electrical conductivity of Pt NWs@g-SBA-15/Thi. With the good cooperation between D-Au film and Pt NWs@g-SBA-15/Thi, a linear relationship between current signals and the concentrations of HBs Ag was obtained in the wide range from 10 fg/mL to 100ng/mL and the detection limit of HBs Ag was 3.3 fg/mL (signal-to-noise ratio of 3). Furthermore, the designed immunosensor with excellent selectivity, reproducibility and stability shows excellent performance in detection of human serum samples and provides a promising capacity for detecting a wide range of other tumor markers in clinical application.


Assuntos
Técnicas Eletroquímicas/métodos , Antígenos de Superfície da Hepatite B/sangue , Vírus da Hepatite B/isolamento & purificação , Hepatite B/sangue , Nanofios/química , Fenotiazinas/química , Platina/química , Anticorpos Imobilizados/química , Técnicas Biossensoriais/métodos , Ouro/química , Antígenos de Superfície da Hepatite B/análise , Humanos , Imunoensaio/métodos , Limite de Detecção , Nanofios/ultraestrutura , Reprodutibilidade dos Testes , Dióxido de Silício/química
2.
Biosens Bioelectron ; 87: 752-759, 2017 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-27649331

RESUMO

Herein, a novel and sensitive sandwich-type electrochemical immunosensor was fabricated for quantitative monitoring of prostate specific antigen (PSA). The sulfo group functionalized multi-walled carbon nanotubes (MWCNTs-SO3H) were used as substrate material to increase the specific surface area and enhance the conductivity of the glassy carbon electrode. Gold nanoparticles (Au NPs) were introduced to enhance the load capacity of the substrate material for primary antibodies (Ab1) and accelerate the electron transfer on the electrode interface. The mesoporous core-shell Pd@Pt nanoparticle loaded by amino group functionalized graphene (M-Pd@Pt/NH2-GS) with high specific surface area, high indexed facets, and good biocompatibility was not only as the carriers of secondary antibodies (Ab2) but also catalyzed the reduction of hydrogen peroxide (H2O2), which effectually amplified the current signal in detection of PSA. The as-proposed immunosensor exhibited high sensitivity and stability on the detection of PSA. A linear relationship between current signals and the concentrations of PSA was obtained in the range from 10fg/mL to 50ng/mL and the detection limit of PSA was 3.3fg/mL (signal-to-noise ratio of 3). Furthermore, the as-proposed immunosensor showed excellent performance in detection of human serum samples. The results suggest that the proposed immunosensor will be promising in the diagnostics application for accurately quantitative detection of PSA.


Assuntos
Anticorpos Imobilizados/química , Técnicas Eletroquímicas/métodos , Grafite/química , Nanocompostos/química , Paládio/química , Platina/química , Antígeno Prostático Específico/sangue , Aminas/química , Técnicas Biossensoriais/métodos , Ouro/química , Humanos , Imunoensaio/métodos , Limite de Detecção , Masculino , Nanopartículas Metálicas/química , Nanopartículas Metálicas/ultraestrutura , Nanocompostos/ultraestrutura , Nanotubos de Carbono/química , Nanotubos de Carbono/ultraestrutura , Porosidade , Razão Sinal-Ruído
3.
Acta Crystallogr Sect E Struct Rep Online ; 64(Pt 10): o1886, 2008 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-21201098

RESUMO

In the mol-ecule of the title compound, C(15)H(13)FN(2)O, the phenyl and fluorophenyl rings are oriented at a dihedral angle of 77.92 (3)°. The pyrazolidine ring adopts an envelope conformation. An intra-molecular C-H⋯N hydrogen bond results in the formation of a five-membered ring adopting an envelope conformation. In the crystal structure, inter-molecular N-H⋯O and C-H⋯O hydrogen bonds link the mol-ecules. There are C-H⋯π contacts between between aromatic H atoms and the phenyl and fluorophenyl rings. A π-π contact between phenyl rings [centroid-centroid distance = 3.926 (1) Å] is also observed.

4.
Acta Crystallogr Sect E Struct Rep Online ; 64(Pt 3): o616, 2008 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-21201950

RESUMO

Mol-ecules of the title compound, C(24)H(28)O(12), are linked by inter-molecular C-H⋯O hydrogen bonds. Bond lengths and angles are normal.

5.
Acta Crystallogr Sect E Struct Rep Online ; 64(Pt 5): o864, 2008 Apr 18.
Artigo em Inglês | MEDLINE | ID: mdl-21202351

RESUMO

In the title compound, C(11)H(14)O(5)S, there is an intra-molecular C-H⋯O hydrogen bond, for which the C-C-S-O torsion angle involving the acceptor and donor atoms is 2.4 (4)°. The dihedral angle between the benzene ring and the methoxy-carbonyl plane is 52.7 (4)°. In the crystal structure, mol-ecules are linked via inter-molecular C-H⋯O hydrogen bonds, forming a mol-ecular chain along the b axis.

6.
Acta Crystallogr Sect E Struct Rep Online ; 64(Pt 7): o1231, 2008 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-21202868

RESUMO

In the organic mol-ecule of the title compound, C(19)H(25)O(2)P·0.5H(2)O, the benzene rings are oriented at a dihedral angle of 54.04 (3)°. Intra-molecular C-H⋯O hydrogen bonds result in the formation of two five-membered planar rings, which are oriented with respect to the adjacent benzene rings at dihedral angles of 2.66 (3) and 2.79 (3)°. In the crystal structure, inter-molecular O-H⋯O hydrogen bonds link the mol-ecules. The water oxygen atom lies on a twofold rotation axis.

7.
Acta Crystallogr Sect E Struct Rep Online ; 64(Pt 11): o2101, 2008 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-21580965

RESUMO

In the mol-ecule of the title compound, C(16)H(13)F(3)N(2)O·H(2)O, the two benzene rings are oriented at a dihedral angle of 82.55 (3)° and the pyrazole ring adopts an envelope conformation. In the crystal structure, inter-molecular C-H⋯F hydrogen bonds link the mol-ecules into chains.

8.
Acta Crystallogr Sect E Struct Rep Online ; 64(Pt 12): o2479, 2008 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-21581444

RESUMO

The asymmetric unit of the title compound, C(22)H(16)N(2)O(4)·2CH(4)O, contains one half-mol-ecule and a methanol solvent mol-ecule. The aromatic ring is oriented at a dihedral angle of 82.91 (3)° with respect to the planar indole ring systems. In the crystal structure, inter-molecular O-H⋯O and N-H⋯O hydrogen bonds link the mol-ecules into chains along the b axis.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA