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1.
Mol Cells ; 42(4): 363-375, 2019 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-31091557

RESUMO

Fungal sectorization is a complex trait that is still not fully understood. The unique phenotypic changes in sporadic sectorization in mutants of CpBck1, a mitogen-activated protein kinase kinase kinase (MAPKKK) gene, and CpSlt2, a mitogen-activated protein kinase (MAPK) gene, in the cell wall integrity pathway of the chestnut blight fungus Cryphonectria parasitica have been previously studied. Although several environmental and physiological factors cause this sectoring phenotype, genetic variants can also impact this complex morphogenesis. Therefore, RNA sequencing analysis was employed to identify candidate genes associated with sectorization traits and understand the genetic mechanism of this phenotype. Transcriptomic analysis of CpBck1 and CpSlt2 mutants and their sectored progeny strains revealed a number of differentially expressed genes (DEGs) related to various cellular processes. Approximately 70% of DEGs were common between the wild-type and each of CpBck1 and CpSlt2 mutants, indicating that CpBck1 and CpSlt2 are components of the same MAPK pathway, but each component governs specific sets of genes. Functional description of the DEGs between the parental mutants and their sectored progenies revealed several key pathways, including the biosynthesis of secondary metabolites, translation, amino acid metabolism, and carbohydrate metabolism; among these, pathways for secondary metabolism and translation appeared to be the most common pathway. The results of this comparative study provide a better understanding of the genetic regulation of sector formation and suggest that complex several regulatory pathways result in interplays between secondary metabolites and morphogenesis.


Assuntos
Perfilação da Expressão Gênica/métodos , Proteínas Serina-Treonina Quinases/genética , Locos de Características Quantitativas , Saccharomycetales/fisiologia , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Regulação Fúngica da Expressão Gênica , MAP Quinase Quinase Quinases/genética , MAP Quinase Quinase Quinases/metabolismo , Proteínas Quinases Ativadas por Mitógeno/genética , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Mutação , Fenótipo , Proteínas Serina-Treonina Quinases/metabolismo , Saccharomycetales/genética , Metabolismo Secundário , Análise de Sequência de RNA
2.
Int J Syst Evol Microbiol ; 69(2): 493-497, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30566074

RESUMO

A Gram-stain-negative and strictly aerobic bacterium, designated DMHB-2T, was isolated from a sample of seawater collected off the Yellow Sea coast of the Republic of Korea. Cells were short rods and motile by means of a single polar flagellum. Catalase and oxidase activities were positive. Growth occurred at pH 5.5-10.0 (optimum, pH 6.0), 15-45 °C (optimum, 25 °C) and with 1-9 % NaCl (optimum, 3 %). The respiratory quinone was ubiquinone-8 and the major fatty acids were C16 : 0 (17.9 %), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c; 26.1 %) and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c; 37.4 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DMHB-2T belong to the genus Marinobacterium, with the highest 16S rRNA gene sequence similarity of 95.2 % to Marinobacterium zhoushanense KCTC 42782T. The genomic DNA G+C content of strain DMHB-2T was 60.8 mol%. On the basis of the phenotypic, chemotaxonomic and genotypic characteristics presented in this study, strain DMHB-2T is suggested to represent a novel species of the genus Marinobacterium, for which the name Marinobacteriumboryeongense sp. nov. is proposed. The type strain is DMHB-2T (=KACC 19225T=JCM 31902T).


Assuntos
Oceanospirillaceae/classificação , Filogenia , Água do Mar/microbiologia , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Oceanospirillaceae/isolamento & purificação , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Ubiquinona/química
3.
Front Plant Sci ; 9: 1699, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30515186

RESUMO

We have reported 15 agarose gel band patterns of double-stranded RNA (dsRNA) from Trichoderma spp. We describe herein that band pattern IX in Trichoderma harzianum NFCF319, which appeared to be a single band but consisted of two dsRNAs of similar size, was identified as a novel mycovirus, designated Trichoderma harzianum partitivirus 1 (ThPV1). The larger segment (dsRNA1) of the ThPV1 genome comprised 2,289 bp and contained a single open reading frame (ORF) encoding an RNA-dependent RNA polymerase (RdRp). The smaller segment (dsRNA2) consisted of 2,245 bp with a single ORF encoding a capsid protein (CP). Evaluation of the deduced amino acid sequence and phylogenetic analysis indicated that ThPV1 is a new member of the genus Betapartitivirus in the family Partitiviridae. Curing of virus infection by single-sporing generated 31 virus-free single-spore clones. No significant differences in growth rate, conidia production, or pigmentation were observed between ThPV1-infected and -cured isogenic strains. In addition, comparison of the newly ThPV1-transmitted isolates with their ThPV1-cured parental strain showed no significant difference in colony morphology or pigmentation. However, inhibition of growth in co-cultured Pleurotus ostreatus and Rhizoctonia solani by T. harzianum was increased in ThPV1-containing strains compared with ThPV1-cured isogenic strains. Moreover, ß-1,3-glucanase activity was significantly increased in the ThPV1-containing strains. However, no difference in chitinase activity was observed, suggesting that ThPV1 regulates the activity of a specific fungal enzyme.

4.
Viruses ; 10(11)2018 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-30360465

RESUMO

An increasing number of novel mycoviruses have been described in fungi. Here, we report the molecular characteristics of a novel bisegmented double-stranded RNA (dsRNA) virus from the fungus Trichoderma atroviride NFCF394. We designated this mycovirus as Trichoderma atroviride partitivirus 1 (TaPV1). Electron micrographs of negatively stained, purified viral particles showed an isometric structure approximately of 30 nm in diameter. The larger segment (dsRNA1) of the TaPV1 genome comprised 2023 bp and contained a single open reading frame (ORF) encoding 614 amino acid (AA) residues of RNA-dependent RNA polymerase (RdRp). The smaller segment (dsRNA2) consisted of 2012 bp with a single ORF encoding 577 AA residues of capsid protein (CP). The phylogenetic analysis, based on deduced amino acid sequences of RdRp and CP, indicated that TaPV1 is a new member of the genus Alphapartitivirus in the family Partitiviridae. Virus-cured isogenic strains did not show significant changes in colony morphology. In addition, no changes in the enzymatic activities of ß-1,3-glucanase and chitinase were observed in virus-cured strains. To the best of our knowledge, this is the first report of an Alphapartitivirus in T. atroviride.

5.
Artigo em Inglês | MEDLINE | ID: mdl-30133338

RESUMO

We identified a protein spot showing down-regulation in the presence of Cryphonectria hypovirus 1 (CHV1) and tannic acid supplementation as a septin subunit with the highest homology to the Aspergillus nidulans aspA gene, an ortholog of the Saccharomyces cerevisiae Cdc11 gene. To analyze the functional role of this septin component (CpSep1), we constructed its null mutant and obtained a total of eight CpSep1-null mutants from 137 transformants. All CpSep1-null mutants showed retarded growth with fewer aerial mycelia and intense pigmentation on PDAmb plates. When the marginal hyphae were examined, hyper-branching was observed in contrast to the wild type. The inhibition of colonial growth was partially recovered when the CpSep1-null mutants were cultured in the presence of the osmostabilizing sorbitol. Conidia production of the CpSep1-null mutants was significantly increased by at least 10-fold more. Interestingly, the conidial morphology of the CpSep1-null mutants changed to circular in contrast to the typical rod-shaped spores of the wild type, indicating a role of septin in the spore morphology of C. parasitica. However, no differences in the germination process were observed. Virulence assays using excised chestnut bark, stromal formation on chestnut stems, and apple inoculation indicated that the CpSep1 gene is important in pathogenicity.

6.
Front Plant Sci ; 9: 103, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29456549

RESUMO

Mutation in CpBck1, an ortholog of the cell wall integrity mitogen-activated protein kinase kinase kinase (MAPKKK) of Saccharomyces cerevisiae, in the chestnut blight fungus Cryphonectria parasitica resulted in a sporadic sectorization as culture proceeded. The progeny from the sectored area maintained the characteristics of the sector, showing a massive morphogenetic change, including robust mycelial growth without differentiation. Epigenetic changes were investigated as the genetic mechanism underlying this sectorization. Quantification of DNA methylation and whole-genome bisulfite sequencing revealed genome-wide DNA methylation of the wild-type at each nucleotide level and changes in DNA methylation of the sectored progeny. Compared to the wild-type, the sectored progeny exhibited marked genome-wide DNA hypomethylation but increased methylation sites. Expression analysis of two DNA methyltransferases, including two representative types of DNA methyltransferase (DNMTase), demonstrated that both were significantly down-regulated in the sectored progeny. However, functional analysis using mutant phenotypes of corresponding DNMTases demonstrated that a mutant of CpDmt1, an ortholog of RID of Neurospora crassa, resulted in the sectored phenotype but the CpDmt2 mutant did not, suggesting that the genetic basis of fungal sectorization is more complex. The present study revealed that a mutation in a signaling pathway component resulted in sectorization accompanied with changes in genome-wide DNA methylation, which suggests that this signal transduction pathway is important for epigenetic control of sectorization via regulation of genes involved in DNA methylation.

7.
Int J Syst Evol Microbiol ; 67(12): 5019-5025, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29022543

RESUMO

A Gram-stain-negative, non-motile and facultative anaerobic bacterium, designated CAM-8T, was isolated from an artificial fountain at Chonbuk National University, South Korea. The novel strain grew at 20-37 °C (optimum 25 °C), pH 5.5-7.0 (optimum 6.0) and with 0-2 % NaCl (optimum 0 %). Oxidase and catalase activities were positive. The cell morphology of strain CAM-8T was atypical rods 0.6-0.8 µm in width and 4.5-6.5 µm in length, with a peaked tip and sometimes a bulb shape. CAM-8T existed as single cells, and as pairs or chains of cells. The phylogenetic analysis of 16S rRNA gene sequences indicated that strain CAM-8T clustered with Gemmobacter nectariphilus JCM 11959T and Gemmobactermegaterium JCM 18498T within the genus Gemmobacter. The DNA G+C content of strain CAM-8T was 65.9 mol%. The respiratory quinone was ubiquinone Q-10. The major fatty acids were C18 : 1ω7c and/or C18 : 1ω6c. The polar lipids of strain CAM-8T consisted of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, two uncharacterized phospholipids, an uncharacterized aminolipid, an uncharacterized glycolipid, an uncharacterized aminophospholipid and four uncharacterized lipids. On the basis of phenotypic, phylogenetic and chemotaxonomic data, strain CAM-8T (=KACC 19224T=JCM 31905T) is considered to represent a novel species of the genus Gemmobacter, for which the name Gemmobacter straminiformis sp. nov. is proposed.


Assuntos
Filogenia , Rhodobacteraceae/classificação , Microbiologia da Água , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Fosfolipídeos/química , RNA Ribossômico 16S/genética , República da Coreia , Rhodobacteraceae/genética , Rhodobacteraceae/isolamento & purificação , Análise de Sequência de DNA , Ubiquinona/química
8.
Sci Rep ; 7(1): 9038, 2017 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-28831166

RESUMO

We assessed the biological function of CpSlt2, an ortholog of the cell wall integrity (CWI) MAPK of Saccharomyces cerevisiae, in the chestnut blight fungus Cryphonectria parasitica. The CpSlt2-null mutant exhibited marked changes in colonial growth, near absence of conidiation and aerial hyphae, and abnormal pigmentation. In addition, the CpSlt2-null mutant exhibited CWI-related phenotypic defects including hypersensitivity to cell wall-disturbing agents and other stresses. Electron microscopy revealed the presence of abnormal hyphae such as intrahyphal hyphae. In addition, virulence assays indicated that the CpSlt2 gene plays an important role in fungal pathogenesis. As cultivation of the mutant strains progressed, the majority of the colonies showed sporadic sectorization and mycelia from the sectored area stably maintained the sectored phenotype. Although mycelial growth was partially recovered, the sectored progeny had dramatically impaired virulence, confirming the CpSlt2 gene has a role in pathogenicity. Compared to a previous mutant of the CpBck1 gene, a MAPKKK gene in CWI pathway, the CpSlt2-null mutant showed similar, although not identical, phenotypic changes and most phenotypic changes were less severe than those of the CpBck1-null mutant. These results suggest that the unique sectorization is CWI pathway-specific, though the components in the same CWI pathway have common and specific functions.

9.
J Microbiol ; 55(7): 514-519, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28664517

RESUMO

A Gram stain-negative, yellowish green-pigmented, facultatively anaerobic, motile, curved rod-shaped bacterium designated as strain JJ016T was isolated from an artificial lake in South Korea, and characterized using a polyphasic approach. The 16S rRNA gene sequence of strain JJ016T indicated that the isolate belonged to the family Rhodocyclaceae and exhibited 95.0% identity to Uliginosibacterium gangwonense 5YN10-9T. The major cellular fatty acids of the novel strain were summed feature 3 (C16:1 ω6c and/or C16:1 ω7c), C16:0, C14:0, and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c). The DNA G+C content of strain JJ016T was 61.9 mol%. The major respiratory quinone and major polar lipid of strain JJ016T were ubiquinone-8 and phosphatidylethanolamine, respectively. Based on the morphological and physiological properties and the biochemical evidence presented, we concluded that strain JJ016T represents a novel species of a new genus in the family Rhodocyclaceae, for which the name Viridibacterium curvum gen. nov., sp. nov. is proposed. The type strain is JJ016T (=KACC 16899T =JCM 18715T).


Assuntos
Água Doce/microbiologia , Filogenia , Rhodocyclaceae/classificação , Rhodocyclaceae/isolamento & purificação , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/análise , DNA Bacteriano/genética , Ácidos Graxos/análise , Fosfolipídeos/análise , RNA Ribossômico 16S/genética , República da Coreia , Rhodocyclaceae/genética , Rhodocyclaceae/fisiologia , Análise de Sequência de DNA
10.
J Microbiol ; 55(8): 595-599, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28752297

RESUMO

A Gram-negative, facultative anaerobic, rod-shaped, motile by means of a polar flagellum, greenish-yellow-pigmented bacterial strain (designated strain JJ3220T) was isolated from an artificial lake in South Korea and characterized using a polyphasic approach. The 16S rRNA gene sequence of strain JJ3220T indicated that the isolate belongs to the family Rhodocyclaceae, and that it exhibits 96.4% similarity to Uliginosibacterium paludis KBP-13T. The major cellular fatty acids of the novel strain were C14:0, C16:0, and summed feature 3 (C16:1 ω6c and/or C16:1 ω7c). Strain JJ3220T had flexirubin-type pigments. The DNA G+C content of the strain was 62.8%. The major respiratory quinone and major polar lipid of strain JJ3220T were ubiquinone-8 and phosphatidylethanolamine, respectively. Based on the morphological and physiological properties and biochemical evidence presented, it can be concluded that strain JJ3220T represents a novel species of the genus Uliginosibacterium. The type strain Uliginosibacterium flavum is JJ3220T (=KACC 17644T =JCM 19465T).


Assuntos
Betaproteobacteria/classificação , Betaproteobacteria/isolamento & purificação , Lagos/microbiologia , Anaerobiose , Técnicas de Tipagem Bacteriana , Composição de Bases , Betaproteobacteria/genética , Betaproteobacteria/fisiologia , Análise por Conglomerados , Citosol/química , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Ácidos Graxos/análise , Locomoção , Fosfolipídeos/análise , Filogenia , Pigmentos Biológicos/análise , Quinonas/análise , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
11.
Arch Virol ; 162(4): 1073-1077, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28050736

RESUMO

Molecular characterization of the most common dsRNA element from Trichoderma atroviride indicated that it comprised 8,566 bp and encoded two large open reading frames (ORF1 and 2). The two ORFs were found to overlap by 46 bp with a typical (-1) slippery heptanucelotide sequence. The deduced protein sequences of ORF1 and ORF2 showed significant similarities to those of known mycoviral structural proteins and RNA-dependent RNA polymerases, respectively. Phylogenetic analysis indicated that this dsRNA is a member of a distinct species related to a group of unclassified mycoviruses; therefore, it was named Trichoderma atroviride mycovirus 1 (TaMV1).


Assuntos
Micovírus/isolamento & purificação , RNA de Cadeia Dupla/genética , RNA Viral/genética , Trichoderma/virologia , Sequência de Bases , Micovírus/química , Micovírus/classificação , Micovírus/genética , Genoma Viral , Dados de Sequência Molecular , Conformação de Ácido Nucleico , Fases de Leitura Aberta , Filogenia , RNA de Cadeia Dupla/química , RNA Viral/química , Proteínas Virais/genética
12.
Mycobiology ; 44(3): 155-161, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27790066

RESUMO

The most economically important species used in a wide range of fermentation industries throughout Asia belong to Aspergillus section Flavi, which are morphologically and phylogenetically indistinguishable, with a few being toxigenic and therefore a major concern. They are frequently isolated from Korean fermentation starters, such as nuruk and meju. The growing popularity of traditional Korean alcoholic beverages has led to a demand for their quality enhancement, therefore requiring selection of efficient non-toxigenic strains to assist effective fermentation. This study was performed to classify the most efficient strains of Aspergillus section Flavi isolated from various types of traditional wheat nuruk, based on a polyphasic approach involving molecular and biochemical evaluation. A total of 69 strains were isolated based on colony morphology and identified as Aspergillusoryzae/flavus based on internal transcribed spacer and calmodulin gene sequencing. Interestingly, none were toxigenic based on PCR amplification of intergenic regions of the aflatoxin cluster genes norB-cypA and the absence of aflatoxin in the culture supernatants by thin-layer chromatography analysis. Saccharification capability of the isolates, assessed through α-amylase and glucoamylase activities, revealed that two isolates, TNA24 and TNA15, showed the highest levels of activity. Although the degrees of variation in α-amylase and glucoamylase activities among the isolates were higher, there were only slight differences in acid protease activity among the isolates with two, TNA28 and TNA36, showing the highest activities. Furthermore, statistical analyses showed that α-amylase activity was positively correlated with glucoamylase activity (p < 0.001), and therefore screening for either was sufficient to predict the saccharifying capacity of the Aspergillus strain.

13.
Int J Syst Evol Microbiol ; 66(2): 997-1002, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26643899

RESUMO

A taxonomic study was carried out on a novel bacterial strain, designated MIC2059T, which was isolated from Ungok Wetland of Gochang in Korea. Cells of the isolate were found to be Gram-stain-negative, rod-shaped and motile. Comparison of the 16S rRNA gene sequences indicated that the isolate belonged to the family Rhodocyclaceae, with Uliginosibacterium gangwonense as its closest relative, with a similarity of 94.8 %. It contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0, summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c) and C12 : 0 3-OH as the major fatty acids and Q8 as the respiratory ubiquinone. The polar lipid profile of strain MIC2059T revealed the presence of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol as major polar lipids. In addition, phosphatidylserine, an unidentified aminophospholipid and unidentified lipids were present in small amounts. The DNA G+C content of the strain was 65.1 mol%. On the basis of the evidence presented, it is concluded that strain MIC2059T represents a novel species of a novel genus within the family Rhodocyclaceae, for which the name Niveibacterium umoris gen. nov., sp. nov. is proposed. The type strain is MIC2059T ( = KACC 17062T = JCM 18716T).

14.
Int J Syst Evol Microbiol ; 65(7): 2209-14, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25858251

RESUMO

A novel bacterial strain, designated GCR0105(T), was isolated from a water sample of the Mangyung estuary enclosed by the Saemangeum Embankment, located in JEOLlabuk-do, South Korea. Cells of strain GCR0105(T) were Gram-stain-negative, non-motile and rod-shaped. Colonies of strain GCR0105(T) were pale yellow-pigmented on R2A agar and nutrient agar media, and were able to grow at 15-30 °C (optimum 25 °C) and pH 6.5-8.5 (optimum pH 7.5). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GCR0105(T) was related most closely to Flavisolibacter ginsengisoli Gsoil 643(T) (93.14% similarity). The polar lipid profile of strain GCR0105(T) comprised phosphatidylethanolamine, two unknown aminolipids, an unknown aminophospholipid and four unknown lipids. The DNA G+C content of strain GCR0105(T) was 42.9 mol% and the respiratory quinone was MK-7.On the basis of phenotypic, chemotaxonomic and phylogenetic properties, strain GCR0105(T) represents a novel species in a new genus within the family Chitinophagaceae, for which the name Flaviaesturariibacter amylovorans gen. nov., sp. nov. is proposed. The type strain of Flaviaesturariibacter amylovorans is GCR0105(T) ( = KACC 16454(T) = JCM 17919(T)).


Assuntos
Bacteroidetes/classificação , Estuários , Filogenia , Microbiologia da Água , Técnicas de Tipagem Bacteriana , Bacteroidetes/genética , Bacteroidetes/isolamento & purificação , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Dados de Sequência Molecular , Fosfatidiletanolaminas/química , Pigmentação , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Espermidina/análogos & derivados , Espermidina/química , Amido/metabolismo , Vitamina K 2/análogos & derivados , Vitamina K 2/química
15.
Int J Syst Evol Microbiol ; 64(Pt 11): 3838-43, 2014 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25168612

RESUMO

A taxonomic study was carried out on a novel bacterial strain, designated MIC2002(T), which was isolated from Wibong falls in Korea. Cells were Gram-stain-negative, aerobic, non-motile and rods, 0.3-0.5 µm wide and 4.0-5.0 µm long. The optimum temperature and pH range for growth were 25 °C and pH 6.5-7.0, respectively. Catalase and oxidase activities were positive. Flexirubin pigments were not produced. Comparison of the 16S rRNA gene sequences indicated that the isolate belonged to the genus Pedobacter, with Pedobacter daechungensis as its closest relative, with a similarity of 94.4%. It contained iso-C(15:0), anteiso-C(15:0), C(16:0), summed feature 3 (C(16:1)ω6c and/or C(16:1)ω7c) and iso-C(17:0) 3-OH as the major fatty acids and menaquinone MK-7 as isoprenoid quinone. The polar lipid profile of strain MIC2002(T) revealed the presence of phosphatidylethanolamine and an unknown lipid. The DNA G+C content of the strain was 34.7 mol%. On the basis of the evidences presented, it was concluded that strain MIC2002(T) represents a novel species of the genus Pedobacter within the family Sphingobacteriaceae, for which the name Pedobacter pituitosus sp. nov. is proposed. The type strain is MIC2002(T) ( =KACC 17064(T) =JCM 18729(T)).


Assuntos
Pedobacter/classificação , Filogenia , Microbiologia da Água , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Água Doce/microbiologia , Dados de Sequência Molecular , Pedobacter/genética , Pedobacter/isolamento & purificação , Fosfatidiletanolaminas/química , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/química
16.
Int J Syst Evol Microbiol ; 64(Pt 10): 3467-72, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25048209

RESUMO

A taxonomic study was carried out on a novel bacterial strain, designated MIC1008(T), which was isolated from the Gomso salt pan, located in Buan County, Korea. Cells were Gram-stain-negative, aerobic, non-motile and moderately halophilic rods, 0.2-0.4 µm wide and 0.7-1.2 µm long. The optimum temperature and pH for growth were 25 °C and pH 6.5-8.5, respectively. Catalase and oxidase activities were positive. Carotenoid pigments were produced. Comparison of 16S rRNA gene sequences indicated that the isolate belonged to the genus Psychroflexus, with Psychroflexus halocasei WCC 4520(T) as its closest relative, with a 16S rRNA gene sequence similarity of 95.4%. The isolate contained iso-C15:0, iso-C15:1 G, iso-C17:0 3-OH, anteiso-C15:0 and iso-C15:0 3-OH as the major fatty acids and menaquinone MK-6 as the isoprenoid quinone. The major polar lipid profile of strain MIC1008(T) revealed the presence of phosphatidylethanolamine, an unidentified aminolipid and two unidentified lipids. The DNA G+C content of the isolate was 32.9 mol%. On the basis of the evidences presented, it is concluded that strain MIC1008(T) represents a novel species of the genus Psychroflexus within the family Flavobacteriaceae, for which the name Psychroflexus http://dx.doi.org/10.1601/nm.8182 salarius sp. nov. is proposed. The type strain is MIC1008(T) ( = KACC 17063(T) = DSM 25661(T)).


Assuntos
Flavobacteriaceae/classificação , Filogenia , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Flavobacteriaceae/genética , Flavobacteriaceae/isolamento & purificação , Dados de Sequência Molecular , Fosfatidiletanolaminas/química , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/química
17.
Fungal Genet Biol ; 61: 42-9, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24161728

RESUMO

The mpkB gene of Aspergillus nidulans encodes a MAP kinase homologous to Fus3p of Saccharomyces cerevisiae which is involved in conjugation process. MpkB is required for completing the sexual development at the anastomosis and post-karyogamy stages. The mpkB deletion strain could produce conidia under the repression condition of conidiation such as sealing and even in the submerged culture concomitant with persistent brlA expression, implying that MpkB might have a role in timely regulation of brlA expression. The submerged culture of the deletion strain showed typical autolytic phenotypes including decrease in dry cell mass (DCM), disorganization of mycelial balls, and fragmentation of hyphae. The chiB, engA and pepJ genes which are encoding cell wall hydrolytic enzymes were transcribed highly in the submerged culture. Also, we observed that the enzyme activity of chitinase and glucanase in the submerged culture of mpkB deletion strain was much higher than that of wild type. The deletion of mpkB also caused a precocious germination of conidia and reduction of spore viability. The expression of the vosA gene, a member of velvet gene family, was not observed in the mpkB deletion strain. These results suggest that MpkB should have multiple roles in germination and viability of conidia, conidiation and autolysis through regulating the expression of vosA and brlA.


Assuntos
Aspergillus nidulans/enzimologia , Aspergillus nidulans/crescimento & desenvolvimento , Autólise , Quinases de Proteína Quinase Ativadas por Mitógeno/metabolismo , Esporos Fúngicos/crescimento & desenvolvimento , Aspergillus nidulans/citologia , Aspergillus nidulans/genética , Deleção de Genes , Regulação Fúngica da Expressão Gênica , Hifas/citologia , Hifas/crescimento & desenvolvimento , Viabilidade Microbiana , Quinases de Proteína Quinase Ativadas por Mitógeno/genética , Esporos Fúngicos/citologia
18.
Int J Syst Evol Microbiol ; 63(Pt 12): 4663-8, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23934250

RESUMO

A Gram-reaction-negative, rod-shaped, non-motile, red-pigmented bacterium, designated strain GSR3061(T), was isolated from a water sample of the Mangyung estuary enclosed by the Saemangeum Embankment in JEOLlabuk-do of South Korea, and characterized using a polyphasic approach. 16S rRNA gene sequence analysis of strain GSR3061(T) indicated that the isolate belonged to the phylum Bacteroidetes and exhibited similarity levels of 94.7 % to Rufibacter tibetensis NRRL B-51285(T), 92.4 % to Adhaeribacter terreus KACC 14257(T) and 91.9 % to Pontibacter korlensis KACC 15371(T). Growth was observed at 15-40 °C and pH 6.5-9.5. The major cellular fatty acids of the novel strain were summed feature 4 (comprising iso-C17 : 1 I and/or anteiso-C17 : 1 B), iso-C15 : 0, C17 : 1ω6c and iso-C16 : 1 H. Flexirubin-type pigments were absent. The DNA G+C content of strain GSR3061(T) was 44.9 mol% and the major quinone was MK-7. The polar lipid profile consisted mainly of phosphatidylethanolamine; three unidentified lipids, two unknown aminolipids, two unknown phospholipids, an unknown aminophospholipid and an unknown glycolipid were also present. On the basis of the evidence presented, it is concluded that strain GSR3061(T) represents a novel species of a new genus, for which the name Nibribacter koreensis gen. nov., sp. nov. is proposed. The type strain of Nibribacter koreensis is GSR3061(T) ( = KACC 16450(T) = JCM 17917(T)).


Assuntos
Cytophagaceae/classificação , Estuários , Filogenia , Microbiologia da Água , Técnicas de Tipagem Bacteriana , Composição de Bases , Cytophagaceae/genética , Cytophagaceae/isolamento & purificação , DNA Bacteriano/genética , Ácidos Graxos/química , Dados de Sequência Molecular , Fosfolipídeos/química , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/química
19.
Int J Syst Evol Microbiol ; 63(Pt 12): 4508-14, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23907222

RESUMO

Two strains, designated GCR0103(T) and GYR3121(T), were isolated from seawater of the Saemangeum Embankment in Jeollabuk-do, Korea. The cells of the two strains were Gram-reaction-negative and non-motile, and formed multicellular filaments. The colonies of the two strains were pink-pigmented and able to grow at 15-37 °C (optimum 25 °C) on R2A and NA medium. Strains GCR0103(T) and GYR3121(T) grew at pH 6.5-10 (optimum pH 7.5) and pH 5.5-9.5 (optimum pH 7.5), and within NaCl ranges of 0-0.4% and 0-1%, respectively. The polar lipid profiles of the two strains contained phosphatidylethanolamine, five unknown aminolipids, an unknown phospholipid and four or five unknown lipids. The DNA G+C contents of strains GCR0103(T) and GYR3121(T) were 56.0 and 54.5 mol%, respectively. The respiratory quinone detected in both strains was MK-7. The 16S rRNA gene sequence similarity between GCR0103(T) and GYR3121(T) was 95.5 %. The 16S rRNA gene sequence similarities of the two strains to closely related reference strains were less than 89 %. Phylogenetic analysis based on 16S rRNA genes showed that GCR0103(T) and GYR3121(T) formed a distinct phyletic line in the family Cytophagaceae. On the basis of the phenotypic, chemotaxonomic and phylogenetic properties, strains GCR0103(T) and GYR3121(T) represent two novel species in a new genus within the family Cytophagaceae, for which the names Nibrella saemangeumensis gen. nov., sp. nov. and Nibrella viscosa sp. nov. are proposed. The type strain of Nibrella saemangeumensis is GCR0103(T) ( = KACC 16453(T) = JCM 17927(T)) and the type strain of Nibrella viscosa is GYR3121(T) ( = KACC 16447(T) = JCM 17925(T)).


Assuntos
Cytophagaceae/classificação , Filogenia , Água do Mar/microbiologia , Técnicas de Tipagem Bacteriana , Composição de Bases , Cytophagaceae/genética , Cytophagaceae/isolamento & purificação , DNA Bacteriano/genética , Ácidos Graxos/química , Dados de Sequência Molecular , Fosfatidiletanolaminas/química , Fosfolipídeos/química , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Espermidina/química , Vitamina K 2/análogos & derivados , Vitamina K 2/química
20.
Int J Syst Evol Microbiol ; 63(Pt 12): 4568-73, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23907229

RESUMO

Two Gram-reaction-negative, rod-shaped, non-motile and red-pink-pigmented bacterial strains, designated GYR3077(T) and GSR0100(T), were isolated from a water sample of the Mangyung estuary enclosed by the Saemangeum Embankment in JEOLlabuk-do, South Korea, and were characterized using a polyphasic approach. 16S rRNA genes of strains GYR3077(T) and GSR0100(T) exhibited sequence similarities of 95.9 % to Hymenobacter deserti ZLB-3(T) and 96.6 % to Hymenobacter soli PB17(T), respectively, and indicated that these isolates belonged to the phylum Bacteroidetes. The major cellular fatty acids present in the two isolates were iso-C15 : 0, C16 : 1ω5c, summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B) and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). The major respiratory quinone and polyamine patterns were menaquinone-7 and sym-homospermidine, characteristic of the genus Hymenobacter. Flexirubin-type pigments were absent in both strains. The DNA G+C contents of strains GYR3077(T) and GSR0100(T) were 60.2 mol% and 61.9 mol%, respectively. The major polar lipid of strains GYR3077(T) and GSR0100(T) was phosphatidylethanolamine. Based on the morphological and physiological properties, strains GYR3077(T) and GSR0100(T) were considered to represent two novel species of the genus Hymenobacter, for which the names Hymenobacter koreensis sp. nov. (type strain GYR3077(T) = KACC 16451(T) = JCM 17924(T)) and Hymenobacter saemangeumensis sp. nov. (type strain GSR0100(T) = KACC 16452(T) = JCM 17923(T)) are proposed.


Assuntos
Cytophagaceae/classificação , Estuários , Filogenia , Água do Mar/microbiologia , Técnicas de Tipagem Bacteriana , Composição de Bases , Cytophagaceae/genética , Cytophagaceae/isolamento & purificação , DNA Bacteriano/genética , Ácidos Graxos/química , Dados de Sequência Molecular , Fosfatidiletanolaminas/química , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Espermidina/análogos & derivados , Espermidina/química , Vitamina K 2/análogos & derivados , Vitamina K 2/química
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