Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 11 de 11
Filtrar
Mais filtros










Tipo de estudo
Intervalo de ano de publicação
1.
Eur J Obstet Gynecol Reprod Biol ; 237: 89-92, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31035121

RESUMO

OBJECTIVE: The prevalence of red cell antibodies in pregnancy varies with ethnicity and geographical location, while the obstetric outcome depends on the available standard of care. Despite being the tertiary fetal medicine centre in West Yorkshire, the prevalence of red cell antibodies, and the outcome of pregnancies associated with these antibodies at the Leeds University Teaching Hospitals Trust remains unreported. This article aims to provide this information for the purpose of patient education and counselling. STUDY DESIGN: The data of pregnant women with red cell antibodies between January 2011 and December 2016 was obtained from the Trust's database and reconciled with the Fetal Medicine Unit records using Viewpoint©. Fetal anaemia requiring in utero transfusion (IUT) was defined as a Middle Cerebral Artery Peak Systolic Velocities ≥ 1.5multiple of the median expected for gestational age. The mean gestational age at delivery, and perinatal outcomes of the pregnancies were recorded. RESULT: Overall, 398 of the 96, 692 pregnant women that were screened had red cell antibodies, giving a prevalence of 1: 242 pregnancies. The Anti- E and Anti-M antibodies were the most common (114 women; 28.6%, and 112 women; 28.1% respectively), but did not cause fetal anaemia in isolation, while anti-D alloimmunization was the predominant indication for in-utero transfusion (IUT). Anti-DE and anti-Kell antibodies had the highest mean number of transfusions per pregnancy. The mean gestational age at delivery was 34 ± 2weeks. Post-transfusion fetal demise was recorded in two hydropic fetuses, both at a gestational age of 25 weeks; giving a transfusion-related mortality rate of 2.5%. CONCLUSION: The prevalence of red cell antibodies at West Yorkshire is lower compared with reports from other Caucasian populations.Nevertheless, these antibodies are important causes of iatrogenic preterm delivery and fetal morbidity. The prognosis is however good with prompt diagnosis and management.


Assuntos
Sistema do Grupo Sanguíneo ABO/imunologia , Autoanticorpos , Isoimunização Rh/epidemiologia , Sistema do Grupo Sanguíneo Rh-Hr/imunologia , Adulto , Inglaterra , Feminino , Idade Gestacional , Hospitais de Ensino , Humanos , Recém-Nascido , Gravidez , Resultado da Gravidez , Prevalência
2.
J Obstet Gynaecol ; 39(3): 328-334, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30714504

RESUMO

Molecular diagnostic investigations, following the identification of foetal abnormalities, are routinely performed using array comparative genomic hybridisation (aCGH). Despite the utility of this technique, contemporary approaches for the detection of copy number variation are typically based on next-generation sequencing (NGS). We sought to compare an in-house NGS-based workflow (CNVseq) with aCGH, for invasively obtained foetal samples from pregnancies complicated by foetal structural abnormality. DNA from 40 foetuses was screened using both 8 × 60 K aCGH oligoarrays and low-coverage whole genome sequencing. Sequencer-compatible libraries were combined in a ten-sample multiplex and sequenced using an Illumina HiSeq2500. The mean resolution of CNVseq was 29 kb, compared to 60 kb for aCGH analyses. Four clinically significant, concordant, copy number imbalances were detected using both techniques, however, genomic breakpoints were more precisely defined by CNVseq. This data indicates CNVseq is a robust and sensitive alternative to aCGH, for the prenatal investigation of foetuses with structural abnormalities. Impact statement What is already known about this subject? Copy number variant analysis using next-generation sequencing has been successfully applied to investigations of tumour specimens and patients with developmental delays. The application of our approach, to a prospective prenatal diagnosis cohort, has not hitherto been assessed. What do the results of this study add? Next-generation sequencing has a comparable turnaround time and assay sensitivity to copy number variant analysis performed using array CGH. We demonstrate that having established a next-generation sequencing facility, high-throughput CNVseq sample processing and analysis can be undertaken within the framework of a regional diagnostic service. What are the implications of these findings for clinical practice and/or further research? Array CGH is a legacy technology which is likely to be superseded by low-coverage whole genome sequencing, for the detection of copy number variants, in the prenatal diagnosis of structural abnormalities.


Assuntos
Hibridização Genômica Comparativa/normas , Variações do Número de Cópias de DNA , Sequenciamento de Nucleotídeos em Larga Escala/normas , Diagnóstico Pré-Natal/métodos , Feminino , Humanos , Gravidez , Estudos Prospectivos
3.
Prenat Diagn ; 37(10): 1001-1007, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28753246

RESUMO

OBJECTIVE: To describe data on congenital lung anomalies identified on prenatal ultrasound from two centres in the North of England. METHOD: This retrospective case series includes all cases notified to the Northern Congenital Abnormality Survey from 1990 to 2010 and to Leeds Regional Fetal Medicine Unit 2000-2015. RESULTS: There were a total of 228 cases, 101 from Northern Congenital Abnormality Survey and 127 from Leeds Regional Fetal Medicine Unit. Eight-five per cent were unilateral congenital pulmonary airway malformations (CPAMs), 2% bilateral CPAMs and 11% bronchopulmonary sequestrations. Mediastinal shift was present in 50% of cases, occurring more frequently in macrocystic CPAMs and larger lesions. Polyhydramnios was evident in 28%, and fetal hydrops occurred in 9%. Prenatal regression occurred in 54%, and lesions were no longer visible on ultrasound in 27% at a later gestation. Prenatal intervention was performed in 5% of cases, and postnatal surgical intervention was required in 12% due to ongoing respiratory symptoms. Regression of fetal hydrops occurred in five cases. CONCLUSION: The outcome was favourable in 83% cases. Prenatal regression was common, and macrocystic lesions were less likely to regress than microcystic lesions. No specific prenatal features predicted the need for early surgical intervention. The data analysed in this prenatal series will help when counselling prenatal cases. © 2017 John Wiley & Sons, Ltd.


Assuntos
Doenças Fetais/diagnóstico por imagem , Anormalidades do Sistema Respiratório/diagnóstico por imagem , Anormalidades do Sistema Respiratório/embriologia , Aconselhamento , Inglaterra , Feminino , Idade Gestacional , Humanos , Pulmão/anormalidades , Pulmão/diagnóstico por imagem , Pulmão/embriologia , Gravidez , Resultado da Gravidez , Cuidado Pré-Natal/métodos , Prognóstico , Estudos Retrospectivos , Ultrassonografia Pré-Natal
4.
Bipolar Disord ; 19(4): 259-272, 2017 06.
Artigo em Inglês | MEDLINE | ID: mdl-28574156

RESUMO

OBJECTIVES: Individualized treatment for bipolar disorder based on neuroimaging treatment targets remains elusive. To address this shortcoming, we developed a linguistic machine learning system based on a cascading genetic fuzzy tree (GFT) design called the LITHium Intelligent Agent (LITHIA). Using multiple objectively defined functional magnetic resonance imaging (fMRI) and proton magnetic resonance spectroscopy (1 H-MRS) inputs, we tested whether LITHIA could accurately predict the lithium response in participants with first-episode bipolar mania. METHODS: We identified 20 subjects with first-episode bipolar mania who received an adequate trial of lithium over 8 weeks and both fMRI and 1 H-MRS scans at baseline pre-treatment. We trained LITHIA using 18 1 H-MRS and 90 fMRI inputs over four training runs to classify treatment response and predict symptom reductions. Each training run contained a randomly selected 80% of the total sample and was followed by a 20% validation run. Over a different randomly selected distribution of the sample, we then compared LITHIA to eight common classification methods. RESULTS: LITHIA demonstrated nearly perfect classification accuracy and was able to predict post-treatment symptom reductions at 8 weeks with at least 88% accuracy in training and 80% accuracy in validation. Moreover, LITHIA exceeded the predictive capacity of the eight comparator methods and showed little tendency towards overfitting. CONCLUSIONS: The results provided proof-of-concept that a novel GFT is capable of providing control to a multidimensional bioinformatics problem-namely, prediction of the lithium response-in a pilot data set. Future work on this, and similar machine learning systems, could help assign psychiatric treatments more efficiently, thereby optimizing outcomes and limiting unnecessary treatment.


Assuntos
Sintomas Comportamentais , Transtorno Bipolar , Resistência a Medicamentos , Compostos de Lítio , Imagem por Ressonância Magnética/métodos , Espectroscopia de Prótons por Ressonância Magnética/métodos , Adolescente , Adulto , Antimaníacos/administração & dosagem , Antimaníacos/efeitos adversos , Inteligência Artificial , Sintomas Comportamentais/diagnóstico , Sintomas Comportamentais/tratamento farmacológico , Transtorno Bipolar/diagnóstico , Transtorno Bipolar/tratamento farmacológico , Transtorno Bipolar/psicologia , Manual Diagnóstico e Estatístico de Transtornos Mentais , Monitoramento de Medicamentos/métodos , Feminino , Lógica Fuzzy , Humanos , Compostos de Lítio/administração & dosagem , Compostos de Lítio/efeitos adversos , Masculino , Imagem Multimodal/métodos , Projetos Piloto , Valor Preditivo dos Testes , Prognóstico
5.
Ciênc. rural ; 41(4): 592-598, abr. 2011. ilus, tab
Artigo em Português | LILACS-Express | ID: lil-585992

RESUMO

O objetivo deste trabalho foi determinar a concentração de polifenóis totais, flavonóides, vitamina C e carotenóides totais em 61 acessos de banana pertencentes ao Banco Ativo de Germoplasma de bananeira da Embrapa Mandioca e Fruticultura. Foram detectadas diferenças significativas para todas as características analisadas por meio da análise de variância. A média para os teores de polifenóis totais entre os 61 acessos de banana foi de 38,06mg 100g-1, com variação de 12,51mg 100g-1 para o triplóide 'Torp' a 257,80mg 100g-1 para o tetraplóide 'Teparod'. A média para flavonóides foi de 2,09mg 100g-1, variando de 0,85mg 100g-1 ('Maravilha' AAAB) a 6,63mg 100g-1 ('Teparod' ABBB). Para vitamina C, a média foi de 21,60mg 100g-1, variando de 8,60mg 100g-1 (tetraplóide 'Bucaneiro') a 76,82mg 100g-1 (tetraplóide 'Teparod'). A média do teor de carotenóides totais foi de 4,34mg g-1, variando de 1,18mg g-1 ('Champa Madras', triplóide ABB) a 19,24mg g-1 ('Saney', triplóide AAB). Os diplóides Modok Gier e NBA-14 apresentaram, respectivamente, quatro e cinco vezes mais carotenóides totais que as cultivares do grupo Cavendish ('Nanica', 'Willians' e 'Lacatan'). Esses resultados permitem concluir sobre a possibilidade da obtenção de cultivares com altos níveis de compostos funcionais por meio de cruzamentos e seleção. Cultivares com este perfil têm potencial como alimento funcional, ajudando a prevenir doenças, por meio da neutralização de radicais livres.


The objective of this research was to determine the concentration of polyphenols, flavonols, vitamin C and carotenoid and in 61 banana accessions from Musa germplasm collection from Embrapa Cassava and Fruits, Brazil. Was detected significant differences for all characteristic analyzed in ANOVA. The average for polyphenols among the 61 accessions was of 38.06mg 100g-1, with variation of 12.51mg 100g-1 for the triploid 'Torp' to 257.80mg 100g-1 for the tetraploid 'Teparod'. The average for flavonols was of 2.09mg 100g-1, ranging of 0.85mg 100g-1 ('Maravilha' AAAB) to 6.63mg 100g-1 ('Teparod' ABBB). The average for vitamin C was of 21.60mg 100g-1, ranging of 8.60mg 100g-1 (tetraploid 'Bucaneiro') to 76.82mg 100g-1 (tetraploid 'Teparod'). The average of the total carotenoids was of 4.34g g-1, ranging of 1.18g g-1 ('Champa Madras', triploid ABB) to 19.24g g-1 ('Saney', triploid AAB). Diploids Modok Gier and NBA-14 exhibited 4- and 5-fold increased carotenoid content in comparison to the representatives from the Cavendish group ('Nanica', 'Willians' and 'Lacatan'). These results allow ending about the possibility of the obtaining new cultivars with high levels of functional compositions through crossings and selection. Cultivar with this profile has potential of neutralizing free radicals and to prevent diseases.

6.
Rev. Inst. Adolfo Lutz ; 70(1): 41-46, jan.-mar. 2011. tab, graf
Artigo em Português | LILACS, SESSP-CTDPROD, Sec. Est. Saúde SP, SESSP-ACVSES, SESSP-IALPROD, Sec. Est. Saúde SP, SESSP-IALACERVO | ID: lil-616837

RESUMO

O tucupi, produto amplamente consumido pela população do norte do Brasil, é obtido por meio de fermentação e cocção da manipueira, que é um subproduto da fabricação da farinha de mandioca seca. No processamento são utilizadas as raízes de mandioca que contêm elevado teor de ácido cianídrico (HCN), as quais quando ingeridas causam sério dano à saúde. No presente trabalho foram quantificados os teores de cianeto total e livre durante as etapas de processamento da manipueira desde a obtenção até a produção do tucupi. Além disso, foram determinados pH, acidez total titulável e teor de sólidos solúveis durante todas as etapas de processamento. A manipueira recém-extraída apresentou valores de 227,8 mg HCN/L de cianeto total e 46,6 mg HCN/L de cianeto livre. Após as etapas de fermentação houve decréscimo no teor de HCN, e o produto final (tucupi) apresentou valores de 37,1 mg HCN/L de cianeto total e 8,9 mg HCN/L de cianeto livre. Quanto aos demais parâmetros, os valores encontrados foram: pH (3,6), acidez total titulável (12,3 meqNaOH/100 mL) e sólidos solúveis (8,1 ºBrix). No presente estudo, o tucupi não apresentou valores elevados de cianeto total e livre após aplicação do processo de fermentação e cocção da manipueira.


Assuntos
Fermentação , Cianeto de Hidrogênio , Manihot
7.
Rev. Inst. Adolfo Lutz ; 70(1): 41-46, jan.-mar. 2011. tab, graf
Artigo em Português | Sec. Est. Saúde SP, SESSP-CTDPROD, Sec. Est. Saúde SP, SESSP-ACVSES, SESSP-IALPROD, Sec. Est. Saúde SP, SESSP-IALACERVO | ID: ses-21307

RESUMO

O tucupi, produto amplamente consumido pela população do norte do Brasil, é obtido por meio de fermentação e cocção da manipueira, que é um subproduto da fabricação da farinha de mandioca seca. No processamento são utilizadas as raízes de mandioca que contêm elevado teor de ácido cianídrico (HCN), as quais quando ingeridas causam sério dano à saúde. No presente trabalho foram quantificados os teores de cianeto total e livre durante as etapas de processamento da manipueira desde a obtenção até a produção do tucupi. Além disso, foram determinados pH, acidez total titulável e teor de sólidos solúveis durante todas as etapas de processamento. A manipueira recém-extraída apresentou valores de 227,8 mg HCN/L de cianeto total e 46,6 mg HCN/L de cianeto livre. Após as etapas de fermentação houve decréscimo no teor de HCN, e o produto final (tucupi) apresentou valores de 37,1 mg HCN/L de cianeto total e 8,9 mg HCN/L de cianeto livre. Quanto aos demais parâmetros, os valores encontrados foram: pH (3,6), acidez total titulável (12,3 meqNaOH/100 mL) e sólidos solúveis (8,1 ºBrix). No presente estudo, o tucupi não apresentou valores elevados de cianeto total e livre após aplicação do processo de fermentação e cocção da manipueira. (AU)


Assuntos
Cianeto de Hidrogênio , Fermentação , Manihot
8.
Acta amaz ; 41(2): 279-284, 2011. tab
Artigo em Português | LILACS-Express | ID: lil-586484

RESUMO

A etapa de fermentação na produção da farinha de mandioca do grupo d'água é responsável pelo desenvolvimento de características químicas e sensoriais peculiares no produto. O objetivo deste estudo foi verificar a influência da etapa de fermentação das raízes de mandioca nos principais parâmetros físico-químicos da farinha, seguido de avaliação sensorial da preferência do consumidor. As análises realizadas foram determinação do teor de umidade, cinzas, proteínas, amido e acidez total titulável. O teste sensorial utilizado foi o de ordenação-preferência dos produtos. De acordo com os resultados obtidos, a etapa de fermentação, responsável pelo aumento da acidez total titulável da farinha de mandioca do grupo d'água, foi o único parâmetro que excedeu o valor limite permitido pela legislação brasileira vigente. Com base no resultado da análise sensorial, houve maior preferência dos consumidores pela farinha de mandioca com maior teor de acidez total (3,44 cmol NaOH kg-1), que corresponde à raiz fermentada por 96 horas.


The fermentation stage in the production of fermented cassava flour (also known as farinha d'água in Brazil) is responsible for the development of chemical and sensorial characteristics in the product. Thus the aim of this study was to verify the influence of the fermentation stage in the flour's main physicochemical parameters, followed by sensory evaluation of customer preference. The analyses performed were moisture, ash, protein, starch amounts and total acidity. The sensory analysis of the produced flour was performed using the preference-ordering test. According to the results, the fermentation stage, responsible for the increase of total acidity content in the final product, was the only parameter which exceeds the value demanded by Brazilian legislation. Based on the result of sensorial analysis, most customers preferred cassava flour with the highest amount of total acidity (3.44 cmol NaOH kg-1), which correspond to the root fermented for 96 hours.

9.
Acta amaz ; 40(1): 221-226, mar. 2010. ilus
Artigo em Português | LILACS-Express | ID: lil-546978

RESUMO

O objetivo deste trabalho foi quantificar a concentração de cianeto total durante as etapas de produção da farinha de mandioca dos grupos seca e d'água. Em relação à farinha seca, a concentração de cianeto total na raiz de mandioca diminuiu de 160±11,8 mg HCN/kg para 149±12,3 mg HCN/kg após a trituração, 68±2,5 mg HCN/kg após a prensagem e chegando a 5±0,2 mg HCN/kg no produto final, após o processo de torração. Na produção da farinha d'água, a raiz de mandioca apresentava teor de cianeto total de 321±21,6 mg HCN/kg e durante o processo de fermentação da raiz, o teor de cianeto total nas primeiras 24 horas de fermentação era de 297±2,7 mg HCN/kg chegando a 64±2,3 mg HCN/kg após 96 horas em repouso no tanque. Após trituração e prensagem da massa fermentada, os valores diminuíram para 50±0,6 e 36±0,4 mg HCN/kg, respectivamente, obtendo-se no produto final a concentração de 9±0,1 mg HCN/kg, sendo evidenciado a eficiência do processo de destoxificação em ambos os processamentos.


The aim of this work was to quantify the total cyanide concentration during the production stages of cassava flour from dry and water groups. In relation to dry flour, the total cyanide concentration in the cassava root reduced from 160±11.8 mg HCN/kg to 149±12.3 mg HCN/kg after grinding, 68±2.5mg HCN/kg after pressing and 5±0.2 mg HCN/kg was obtained in the final product after the roasting process. For the water flour production, the cassava root showed 321±21.6 mg HCN/kg total cyanide content, and during the fermentation process, the total cyanide content for the first 24-hour fermentation was from 297±2.7 mg HCN/kg reaching 64±2.3 mg HCN/kg after resting in a pool for 96 hours. After grinding and pressing the fermented roots, the values lowered to 50±0.6 and 36±0.4 mg HCN/kg, respectively. A concentration of 9±0.1 mg HCN/kg was obtained in the final product, evidencing the detoxification process efficiency in both processings.

10.
Genet Mol Biol ; 32(1): 96-103, 2009 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-21637652

RESUMO

The aim of this work was to evaluate the carotenoid content and genetic variability of banana accessions from the Musa germplasm collection held at Embrapa Cassava and Tropical Fruits, Brazil. Forty-two samples were analyzed, including 21 diploids, 19 triploids and two tetraploids. The carotenoid content was analyzed spectrophotometrically and genetic variability was estimated using 653 DArT markers. The average carotenoid content was 4.73 µg.g (-1) , and ranged from 1.06 µg.g (-1) for the triploid Nanica (Cavendish group) to 19.24 µg.g (-1) for the triploid Saney. The diploids Modok Gier and NBA-14 and the triploid Saney had a carotenoid content that was, respectively, 7-fold, 6-fold and 9-fold greater than that of cultivars from the Cavendish group (2.19 µg.g (-1)). The mean similarity among the 42 accessions was 0.63 (range: 0.24 to 1.00). DArT analysis revealed extensive genetic variability in accessions from the Embrapa Musa germplasm bank.

11.
Genet. mol. biol ; 32(1): 96-103, 2009. ilus, graf, tab
Artigo em Inglês | LILACS | ID: lil-505774

RESUMO

The aim of this work was to evaluate the carotenoid content and genetic variability of banana accessions from the Musa germplasm collection held at Embrapa Cassava and Tropical Fruits, Brazil. Forty-two samples were analyzed, including 21 diploids, 19 triploids and two tetraploids. The carotenoid content was analyzed spectrophotometrically and genetic variability was estimated using 653 DArT markers. The average carotenoid content was 4.73 µg.g-1, and ranged from 1.06 µg.g-1 for the triploid Nanica (Cavendish group) to 19.24 µg.g-1 for the triploid Saney. The diploids Modok Gier and NBA-14 and the triploid Saney had a carotenoid content that was, respectively, 7-fold, 6-fold and 9-fold greater than that of cultivars from the Cavendish group (2.19 µg.g-1). The mean similarity among the 42 accessions was 0.63 (range: 0.24 to 1.00). DArT analysis revealed extensive genetic variability in accessions from the Embrapa Musa germplasm bank.


Assuntos
Carotenoides/análise , Variação Genética , Musa/genética , Marcadores Genéticos , Genótipo , Polimorfismo Genético , Técnica de Amplificação ao Acaso de DNA Polimórfico
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA