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J Sep Sci ; 43(12): 2436-2446, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32227667


Four types of middle-pressure chromatogram isolated gels are evaluated for adsorption or desorption characteristics of ginsenosides from Panax ginseng. Among them, SP207SS and SP2MGS were selected for dynamic investigations based on their static adsorption or desorption capacity of total ginsenoside. Their adsorption kinetics was better explained by pseudosecond-order model and isotherms were preferably fitted to Langmuir model. Dynamic breakthrough experiments indicated an optimum sample loading speed of 4 bed volume/h for either SP207SS or SP2MGS. Desorption speed was determined to be 2 bed volume/h according to desorption amount of total ginsenoside in their effluents. Eight ginsenosides were identified and quantified by high performance liquid chromatography-triple quadropole-mass spectrometry in total ginsenoside extract and different fractions during stepwise dynamic elution. For SP207SS, 27.62% of loaded ginsenosides was detected in 40% ethanol fraction, while 59.12% of them were found in 60% ethanol fraction. As on SP2MGS, the number went to 53.71 and 44.43%, respectively. Recovery rate of ginsenosides were calculated to 78.65% for SP207SS and 89.53% for SP2MGS, respectively. Intriguingly, content of Rg1 and Re in 40% ethanol fraction from SP207SS became 20.1 and 18.6 times higher than that in total ginsenoside extract by one-step elution, which could be leveraged for the facile enrichment of these two ginsenosides from natural sources.

Dermatol Nurs ; 14(6): 377-83; quiz 384, 2002 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-12592793


The skin reflects the internal well-being of the body and can develop manifestations of systemic illness. The nurse's ability to recognize and accurately describe lesions can lead to prompt diagnosis and treatment of conditions, helping the patient to avoid discomfort, systemic illness, or death. To accurately describe skin findings, the nurse should know the names and defining characteristics of certain primary lesions.

Dermatologia/métodos , Anamnese/métodos , Exame Físico/enfermagem , Dermatopatias/diagnóstico , Cabelo/patologia , Humanos , Avaliação em Enfermagem , Exame Físico/métodos , Couro Cabeludo/patologia , Dermatopatias/patologia