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1.
Cad. saúde colet., (Rio J.) ; 27(3): 345-353, jul.-set. 2019. tab, graf
Artigo em Português | LILACS-Express | ID: biblio-1039442

RESUMO

Resumo Introdução A saúde pública no Brasil sofreu grandes mudanças nas últimas décadas. Objetivo Descrever o panorama da produção odontológica realizada pelo SUS de 1999 a 2017 no Brasil e suas macrorregiões. Método Os dados foram obtidos no Sistema de Informação Ambulatorial (SIA-SUS) e do Instituto Brasileiro de Geografia e Estatística (IBGE). Foram criadas taxas de procedimentos (por 100 mil habitantes/ano) realizados em cada macrorregião: procedimentos restauradores, protéticos, coletivos, endodontia, exodontia, periodontia e preventivos de 1999 a 2017. A análise estatística das séries temporais foi realizada utilizando um modelo de regressão linear. Resultados Procedimentos protéticos e de periodontia foram os únicos que apresentaram uma tendência linear positiva em todas as macrorregiões brasileiras (p<0,001). A Endodontia não apresentou tendência positiva no Brasil (p=0,173). Restaurações apresentaram um crescimento na macrorregião Norte (p=0,003) e Centro-Oeste (p<0,001). Exodontias apresentaram na macrorregião Norte uma tendência de aumento (p=0,046) enquanto que, no Centro-Oeste, apresentaram uma diminuição (p=0,049). Procedimentos preventivos (p=0,042) e coletivos (p=0,017) apresentaram uma diminuição da sua produção durante o período. Conclusão A saúde bucal apresentou um grande crescimento dentro do Sistema Único de Saúde nos 19 anos avaliados. Procedimentos de periodontia e de prótese dentária foram aqueles com as maiores tendências de crescimento.


Abstract Background Public health in Brazil has undergone major changes in recent decades. Objective To describe the overview of dental production performed by the Brazilian Unified Health System from 1999 to 2017 in Brazil and its macroregions. Method Data were obtained from the Outpatient Information System (SIA/SUS) and from the Brazilian Institute of Geography and Statistics (IBGE). Procedures rates (per 100,000 inhabitants per year) were established in each macroregion: restorative, prosthetic, collective, endodontic, exodontia, periodontic and preventive procedures. Statistical analysis of the time series was performed using a regression linear model. Results Prosthetic and periodontal procedures were the only ones with a positive linear trend in all Brazilian macroregions (p<0.001). Endodontics did not show a positive trend in Brazil (p=0.173). Restorations showed a growth in the North (p=0.003) and Center-west (p<0.001) macroregions. Exodontia presented a tendency to increase in the North macroregion (p=0.046), while the Midwest presented a decrease of it (p=0.049). Preventive (p=0.042) and collective (p=0.017) procedures showed a decrease in their production during the period. Conclusion Oral health showed great growth within the single health system in the 19 years evaluated. Periodontal procedures and dental prostheses were those with the highest growth trends.

2.
Braz Dent J ; 30(2): 77-95, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30970065

RESUMO

The aim of presented systematic scoping review was to investigate the actual and future clinical possibilities of regenerative therapies and their ability to regenerate bone, periodontal and pulp with histological confirmation of the nature of formed tissue. Electronic search was conducted using a combination between Keywords and MeSH terms in PubMed, Scopus, ISI-Web of Science and Cochrane library databases up to January 2016. Two reviewers conducted independently the papers judgment. Screened studies were read following the predetermined inclusion criteria. The included studies were evaluated in accordance with Arksey and O'Malley's modified framework. From 1349 papers, 168 completed inclusion criteria. Several characterized and uncharacterized cells used in Cell Therapy have provided bone regeneration, demonstrating bone gain in quantity and quality, even as accelerators for bone and periodontal regeneration. Synthetic and natural scaffolds presented good cell maintenance, however polyglycolid-polylactid presented faster resorption and consequently poor bone gain. The Growth Factor-Mediated Therapy was able to regenerate bone and all features of a periodontal tissue in bone defects. Teeth submitted to Revascularization presented an increase of length and width of root canal. However, formed tissues not seem able to deposit dentin, characterizing a repaired tissue. Both PRP and PRF presented benefits when applied in regenerative therapies as natural scaffolds. Therefore, most studies that applied regenerative therapies have provided promising results being possible to regenerate bone and periodontal tissue with histological confirmation. However, pulp regeneration was not reported. These results should be interpreted with caution due to the short follow-up periods.


Assuntos
Polpa Dentária , Tratamento do Canal Radicular , Regeneração Óssea , Dentina , Periodonto
3.
Braz. dent. j ; 30(2): 77-95, Mar.-Apr. 2019. tab, graf
Artigo em Inglês | LILACS-Express | ID: biblio-1001438

RESUMO

Abstract The aim of presented systematic scoping review was to investigate the actual and future clinical possibilities of regenerative therapies and their ability to regenerate bone, periodontal and pulp with histological confirmation of the nature of formed tissue. Electronic search was conducted using a combination between Keywords and MeSH terms in PubMed, Scopus, ISI-Web of Science and Cochrane library databases up to January 2016. Two reviewers conducted independently the papers judgment. Screened studies were read following the predetermined inclusion criteria. The included studies were evaluated in accordance with Arksey and O'Malley's modified framework. From 1349 papers, 168 completed inclusion criteria. Several characterized and uncharacterized cells used in Cell Therapy have provided bone regeneration, demonstrating bone gain in quantity and quality, even as accelerators for bone and periodontal regeneration. Synthetic and natural scaffolds presented good cell maintenance, however polyglycolid-polylactid presented faster resorption and consequently poor bone gain. The Growth Factor-Mediated Therapy was able to regenerate bone and all features of a periodontal tissue in bone defects. Teeth submitted to Revascularization presented an increase of length and width of root canal. However, formed tissues not seem able to deposit dentin, characterizing a repaired tissue. Both PRP and PRF presented benefits when applied in regenerative therapies as natural scaffolds. Therefore, most studies that applied regenerative therapies have provided promising results being possible to regenerate bone and periodontal tissue with histological confirmation. However, pulp regeneration was not reported. These results should be interpreted with caution due to the short follow-up periods.


Resumo O objetivo da presente Scoping review foi investigar as possibilidades clínicas atuais e futuras das terapias regenerativas e sua capacidade de regenerar tecido ósseo, periodontal e polpar em humanos com confirmação histológica da natureza do tecido formado. Uma busca eletrônica foi realizada utilizando uma combinação entre as palavras-chave e termos MeSH nos bancos de dados PubMed, Scopus, ISI-web of Science e Cochrane library até janeiro de 2016. Dois revisores realizaram de forma independente o julgamento dos documentos. Os estudos selecionados foram lidos seguindo os critérios de inclusão predeterminados. Os estudos incluídos foram avaliados de acordo com a estrutura modificada de Arksey e O'Malley. Dos 1349 artigos, 168 preencheram os critérios de inclusão. Várias células caracterizadas e não caracterizadas promoveram regeneração óssea utilizada em terapias celulares, demonstrando ganho ósseo em quantidade e qualidade, de forma rápida para regeneração óssea e periodontal. Os scaffolds sintéticos e naturais apresentaram boa manutenção celular, no entanto o poliglicol-polilácido apresentou uma reabsorção rápida e, consequentemente, pequeno ganho ósseo. A terapia mediada por fatores de crescimento foi capaz de regenerar tecido ósseo e todas as características de um tecido periodontal. Dentes submetidos à revascularização apresentaram aumento do comprimento e largura do canal radicular. No entanto, os tecidos formados não foram capazes de depositar dentina, caracterizando um tecido reparado. Tanto o PRP quanto o PRF parecem apresentar benefícios quando aplicados em terapias regenerativas sendo um bom scaffold natural. Portanto, a maioria dos estudos que aplicaram terapias regenerativas forneceram resultados promissores sendo possível regenerar tecido ósseo e periodontal com confirmação histológica. No entanto, não foi observada regeneração de polpa dental. Estes resultados devem ser interpretados com cautela.

4.
J Esthet Restor Dent ; 31(1): 93-97, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30379397

RESUMO

OBJECTIVE: To evaluate the effect of storage time and temperature on the effectiveness of bleaching agents. METHODS: Enamel slabs were randomly allocated according to the concentration of carbamide peroxide (CP): 10% (CP10) and 16% (CP16). Shade evaluations were conducted at baseline and then after 3 and 12 months of storage at 3 different temperatures: 10°C (±2°C), 25°C (±2°C), and 35°C (±2°C). Objective color evaluation was performed (spectrophotometer CIEL*a*b* system and CIEDE2000) to calculate the color change (ΔE00 ). Subjective evaluation was performed using the VITA classical shade guide followed by shade variation (ΔS). RESULTS: The bleaching effect of different CP concentrations (10% and 16%) at baseline showed no differences with regard to objective (ΔE00 ) and subjective parameters (ΔS) (P > .05). No differences were observed between CP concentrations stored for the same length of time and at the same temperature for either parameter (P > .05). In terms of objective measurement (ΔE00 ), a reduction in the bleaching effect was shown for both CP concentrations (10% and 16%) when stored for 12 months at 35°C, and compared with the baseline (P < .05). As regards the subjective parameters (ΔS), after 12 months, both CP concentrations (10% and 16%) stored at 35°C showed a decrease in bleaching capacity compared to baseline (P < .05). CP10 and CP16 stored at 10°C and 25°C did not suffer any loss in bleaching ability, even after 12 months (P > .05). CONCLUSIONS: The storage of bleaching agents for long periods at high temperatures can reduce the bleaching effectiveness of CP at 10% and 16%. CLINICAL SIGNIFICANCE: Clinicians must be made aware that they should store their bleaching products under proper temperature conditions and use them within an appropriate time frame; otherwise, the bleaching effect could be lost.


Assuntos
Clareadores Dentários , Clareamento Dental , Peróxido de Hidrogênio , Peróxidos , Temperatura Ambiente , Ureia
5.
RFO UPF ; 23(2): 161-167, 24/10/2018.
Artigo em Português | LILACS-Express | ID: biblio-947644

RESUMO

Centros de especialidades odontológicos (CEOs) são estabelecimentos de saúde de âmbito especializado que devem realizar uma quantidade mínima de procedimentos. Objetivos: descrever a produção odontológica especializada e reportar o cumprimento das metas nas capitais brasileiras com CEOs. Materiais e método: foi conduzido um estudo do tipo longitudinal retrospectivo, sendo realizada uma busca por CEOs cadastrados no Cadastro Nacional de Estabelecimentos de Saúde (CNES). A produção odontológica foi pesquisada no Sistema de Informações Ambulatoriais do Sistema Único de Saúde (SIASUS), de maio de 2015 a abril de 2016. Resultados: foram encontrados e considerados elegíveis para o presente estudo 59 CEOs, localizados em 19 capitais brasileiras e no Distrito Federal, sendo 48% CEOs tipo II. Cerca de 730 mil procedimentos especializados foram realizados durante os 12 meses avaliados. Uma taxa de 86% das metas foi cumprida, sendo que cirurgia foi à área com maior cumprimento (92%), seguida de periodontia (89%) e endodontia (76%). Uma das capitais apresentou apenas 33% das metas cumpridas. Três capitais não atingiram nenhuma das metas estabelecidas em procedimentos de endodontia. Conclusão: foi observada uma grande variação no cumprimento das metas entre as capitais com CEOs. Enquanto algumas capitais apresentaram elevado cumprimento das metas, outras exibiram dados preocupantes, principalmente nos procedimentos de endodontia.

6.
Int J Paediatr Dent ; 28(2): 123-139, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29322626

RESUMO

BACKGROUND: Several restorative materials with specific indications are used for filling cavities in primary teeth. AIM: To systematically review the literature in order to investigate the longevity of primary teeth restorations and the reasons for failure. DESIGN: Electronic databases were screened, and eligible studies were hand-searched to find longitudinal clinical studies evaluating the survival of restorations (class I, class II, and crown) placed with different materials in primary teeth with at least one year of follow-up. RESULTS: Thirty-one studies were included, and a high bias risk was observed. Overall, 12,047 restorations were evaluated with 12.5% of failure rate. A high variation on annual failure rate (AFR) was detected (0-29.9%). Composite resin showed the lowest AFRs (1.7-12.9%). Stainless steel crowns (SSC) had the highest success rate (96.1%). Class I restorations and restorations placed using rubber dam presented better AFR. The main reason for failure observed was secondary caries (36.5%). CONCLUSIONS: An elevated number of failures were observed due to recurrent caries, highlighting the need for professionals to work with a health-promoting approach. The high variation on failure rate among the materials can be due to children's behavior during the procedure, which demands short dental appointments and a controlled environment.


Assuntos
Falha de Restauração Dentária , Restauração Dentária Permanente , Dente Decíduo/cirurgia , Criança , Cárie Dentária/cirurgia , Falha de Restauração Dentária/estatística & dados numéricos , Humanos
7.
RGO (Porto Alegre) ; 65(4): 359-367, Oct.-Dec. 2017. graf
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-896042

RESUMO

ABSTRACT Regenerative therapies have been widely developed in dentistry and it is important to incorporate dentists' knowledge of these new therapies into the dental clinic routine. This study reviewed the literature on regenerative therapies and clinical applications. Tissue engineering has contributed to changes in the paradigm of restorative health sciences. Its pillars underpin the techniques of tissue and organ regeneration. Despite the majority of studies in this field being in vitro, a range of preclinical studies and methodologies has been formed using these principles and they are already being used on humans. The use of platelet-rich plasma and platelet-rich fibrin in surgery as natural scaffolds for the reestablishment of bone and periodontal tissue are often reported in the literature and clinical trials using this approach have shown promising results. Stem cells from autologous dental pulp have been successfully applied in bone tissue regeneration using natural collagen scaffold in humans. In addition, revascularization of the root canal already appears in the literature as a promising alternative to apexification. The principle behind this therapy is the use of the blood clot as a scaffold and the migration of stem cells of the apical papilla to regenerate the dental pulp organ. Final considerations: Although still in the early stages, regenerative therapies can now be used in dental practice. Knowledge of the principles governing these therapies should be understood by the dentist for use in clinical practice.


RESUMO Terapias regenerativas vem sendo amplamente desenvolvidas na odontologia e o conhecimento destas novas terapias por parte dos dentistas é importante para que elas sejam incorporadas na rotina clínica odontológica. Assim, este estudo revisou a literatura acerca das terapias regenerativas e suas aplicações clínicas. A engenharia tecidual tem contribuído na mudança do paradigma restaurador das ciências da saúde. Seus pilares embasam as técnicas de regeneração de tecidos e órgãos. Apesar da grande maioria dos estudos neste campo ser in vitro, uma gama de metodologias pré-clínicas foi consolidada e estudos utilizando estes princípios já estão sendo empregados em humanos. A utilização de plasma rico em plaquetas e plasma rico em fibrina como scaffolds naturais em cirurgias para reestabelecimento de tecido ósseo e periodontal são frequentemente relatadas na literatura e ensaios clínicos utilizando esta abordagem demonstram resultados promissores. Células-tronco da polpa dental autólogas já foram aplicadas com sucesso na regeneração de tecido ósseo utilizando scaffold naturais de colágeno em humanos. Além disto, a revascularização do canal radicular já aparece na literatura como uma alternativa promissora frente a opção de apecificação do canal radicular. Esta terapia utiliza como princípio o coágulo sanguíneo como scaffold e a migração das células-tronco da papila apical para regenerar o órgão pulpar. Considerações finais: Apesar de incipientes, as terapias regenerativas já podem ser utilizadas na prática clínica odontológica. O conhecimento dos princípios que regem estas terapias deve ser compreendido pelo dentista para que sejam utilizadas na prática clínica.

8.
Acta Stomatol Croat ; 51(2): 133-140, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28827850

RESUMO

The aim of this study was to evaluate the proliferation and adhesion of mesenchymal cells (3T3/NIH) in Dulbecco's Modified Eagle Medium(DMEM) supplemented with Platelet-Poor Plasma (PPP) in aPlatelet-Rich Fibrin (PRF) scaffold. Human blood was obtained and processed in a centrifuge considering the equation G=1.12xRx(RPM/1000)2 to obtain PRF and PPP.Cell adhesion and maintenance analyses were performed by MTTassays in a 96 well plate withsupplemented DMEM: PPP (90:10) for 24 hours. Besides, the PRF was deposited in a 48 well plate and 10x104 cells were seeded above each PRF (n=3) with 800µl of DMEM: PPP (90:10) and cultured for 7 days. Histological analysis and the immunohistochemical staining for Vimentin were performed. Results were analyzed by one-way ANOVA in Stata12®. A significant decrease (p<0.05) of cells adhesion in relationship to FBSwas observed. However, a similar ability of cell-maintenance for PPP 10% was observed (P>0.05). Fibroblasts culture for 7 days in PRF supplemented with PPP 10% was possible, showing positive staining for Vimentin. Therefore, PPP cell supplementation decreased the initial adhesion of cells but was able to maintain the proliferation of adhered cells and able to support their viability in PRF.It seems that this method has many clinical advantagessince it provides an autologous and natural scaffold with their respective supplement for cell culture by only one process, without using xenogeneic compounds. This could improve the potential of clinical translational therapies based on the use of PRF cultured cells, promoting the regenerative potential for future use in medicine and dentistry.

9.
Rev. ABENO ; 17(1): 8-15, 2017. graf
Artigo em Português | LILACS, BBO - Odontologia | ID: biblio-882229

RESUMO

Os Trabalhos de Conclusão de Curso (TCCs) se tornaram obrigatórios na Faculdade de Odontologia da Universidade Federal de Pelotas (FO-UFPel) a partir de 2006, sendo requisito indispensável para a obtenção do título de Cirurgião-Dentista. A compreensão de como os TCCs estão sendo realizados é imprescindível para um correto direcionamento de estratégias na construção dos currículos de graduação, uma vez que até o momento poucos estudos foram conduzidos com a finalidade de analisar os TCCs realizados nos cursos de Odontologia do Brasil. Desta forma, o objetivo deste estudo foi realizar um levantamento dos dados referentes aos TCCs realizados na FO-UFPel até 2014. As variáveis investigadas foram área de concentração do trabalho e tipos de estudos/metodologia empregada. Além disto foi realizada uma busca nas bases de dados PubMed, SciELO e Google Acadêmico para investigar se o trabalho foi publicado em periódicos científicos e o respectivo Qualis. Os dados foram tabulados e analisados descritivamente. Foram encontrados 474 TCCs, (impressos e em formato digital) sendo que a maioria deles foi realizada na área de Dentística (11,4%), Saúde Coletiva (11,0%) e Cirurgia Oral (10,5%). Do total de TCCs, 25,7% foram revisões narrativas da literatura. Apenas 9% das monografias foram publicadas em periódicos científicos. Portanto, é possível concluir que a maioria dos TCCs realizados na FO-UFPelsão revisões narrativas da literatura e que a taxa de publicação destes trabalhos é pequena. Além disso, recomenda-se que novas estratégias sejam empregadas pelos gestores da universidade para propiciar que os TCCs possam ser referência na difusão do conhecimento à comunidade acadêmica (AU).


The final paper (TCCs) became mandatory in the Faculty of Dentistry, Federal University of Pelotas (FO-UFPel) since 2006, and an indispensable requirement for obtaining DDS title. Understanding how the TCCs are being carried out is essential for proper targeting strategies in the construction of the undergraduate curriculum, since to date few studies were conducted in order to analyze the TCCs made in dentistry courses in Brazil. Thus, the aim of this study was to survey the data for TCCs performed in FO-UFPel by 2014. The variables were: area of work concentration, types of studies/methodology. In addition, a search was conducted in PubMed, SciELO and Google Scholar to investigate whether the work was published in scientific journals and their Qualis conceit. The data were tabulated and analyzed descriptively. 474 TCCs were founded (printed and digital format), and most of them were held in operative dentistry (11.4%) followed by public health (11.0%) and oral surgery (10.5%). 25.7% of TCCs were narrative reviews of the literature. Only 9% of monographs have been published in scientific journals. Therefore, we note that the majority of TCCs made in FO-UFPel are narrative literature reviews and the publication of these works is small. In addition, it is recommended that new strategies are employed by the university managers to provide that the TCCs can be reference in dissemination of knowledge to the academic community (AU).


Assuntos
Currículo/normas , Educação em Odontologia , Avaliação Educacional/métodos , Avaliação da Pesquisa em Saúde , Brasil , Estudos Transversais/métodos , Indicadores de Produção Científica
10.
Braz Dent J ; 27(6): 633-640, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27982171

RESUMO

This systematic review evaluated if different cryopreservation protocols could affect biological properties (Cell survival rate (CSR), proliferation, differentiation, maintenance of stem cell markers) of stem cells obtained from dental tissues (DSC) post-thaw. An electronic search was carried out within PubMed and ISI Web Science by using specific keyword. Two independent reviewers read the titles and abstracts of all reports respecting predetermined inclusion/exclusion criteria. Data were extracted considering the biological properties of previously cryopreserved DSCs and previously cryopreserved dental tissues. DSCs cryopreserved as soon as possible after their isolation presents a CSR quite similar to the non-cryopreserved DSC. Dimethyl sulfoxide (DMSO) [10%] showed good results related to cell recovery post-thaw to cryopreserve cells and tissues for periods of up to 2 years. The cryopreservation of DSC in a mechanical freezer (-80°C) allows the recovery of stem cells post-thaw. The facilities producing magnetic field (MF), demand a lower concentration of cryoprotectant, but their use is not dispensable. It is possible to isolate and cryopreserve dental pulp stem cell (DPSC) from healthy and diseased vital teeth. Cryopreservation of dental tissues for late DSC isolation, combined with MF dispensability, could be valuable to reduce costs and improve the logistics to develop teeth banks.


Assuntos
Criopreservação , Células-Tronco Mesenquimais/citologia , Dente/citologia , Sobrevivência Celular , Humanos
11.
Braz. dent. j ; 27(6): 633-640, Nov.-Dec. 2016. tab, graf
Artigo em Inglês | LILACS | ID: biblio-828053

RESUMO

Abstract This systematic review evaluated if different cryopreservation protocols could affect biological properties (Cell survival rate (CSR), proliferation, differentiation, maintenance of stem cell markers) of stem cells obtained from dental tissues (DSC) post-thaw. An electronic search was carried out within PubMed and ISI Web Science by using specific keyword. Two independent reviewers read the titles and abstracts of all reports respecting predetermined inclusion/exclusion criteria. Data were extracted considering the biological properties of previously cryopreserved DSCs and previously cryopreserved dental tissues. DSCs cryopreserved as soon as possible after their isolation presents a CSR quite similar to the non-cryopreserved DSC. Dimethyl sulfoxide (DMSO) [10%] showed good results related to cell recovery post-thaw to cryopreserve cells and tissues for periods of up to 2 years. The cryopreservation of DSC in a mechanical freezer (-80°C) allows the recovery of stem cells post-thaw. The facilities producing magnetic field (MF), demand a lower concentration of cryoprotectant, but their use is not dispensable. It is possible to isolate and cryopreserve dental pulp stem cell (DPSC) from healthy and diseased vital teeth. Cryopreservation of dental tissues for late DSC isolation, combined with MF dispensability, could be valuable to reduce costs and improve the logistics to develop teeth banks.


Resumo Essa revisão sistemática avaliou se diferentes protocolos de criopreservação podem afetar as propriedades biológicas (taxa de sobrevivência celular, proliferação, diferenciação, manutenção dos marcadores de superfície) de células-tronco isoladas de tecidos dentais (DSC) após o descongelamento. Uma busca eletrônica foi realizada no PubMed e no ISI Web of Science utilizando palavras-chave específicas. Dois revisores independentes avaliaram os títulos e resumos de todos os estudos respeitando critérios de inclusão e exclusão previamente determinados. Os dados foram extraídos considerando as propriedades biológicas de DSC, e DSC isoladas de tecidos previamente criopreservados. DSC criopreservadas logo após seu isolamento apresentaram propriedades biológicas muito semelhantes às observadas em DSC não criopreservadas. Dimetil sulfóxido (DMSO) [10%] demonstrou bons resultados relacionados com a recuperação celular após descongelamento de células e tecidos, por períodos de até 2 anos. A criopreservação de DSC em freezer mecânico (-80 °C) permite a recuperação de células-tronco pós-descongelação. A utilização de freezer com campo magnético (MF), proporciona a utilização de uma menor concentração de crioprotector, mas a sua utilização não é dispensável. É possível isolar e criopreservar e criopreservar células-tronco da polpa dental (DPSC) de dentes vitais saudáveis e doentes. Criopreservação de tecidos dentais após o isolamento de DSC, combinados com MF, podem ser valiosas estratégias para reduzir custos e melhorar a logística no desenvolvimento de bancos de dentes.


Assuntos
Humanos , Criopreservação , Células-Tronco Mesenquimais/citologia , Dente/citologia , Sobrevivência Celular
12.
RFO UPF ; 21(3): 407-413, 15/12/2016.
Artigo em Português | LILACS-Express | ID: biblio-848650

RESUMO

O presente estudo revisou a literatura científica em busca das perspectivas e dos principais desafios enfrentados pelas terapias de regeneração do disco articular. Revisão de literatura: a disfunção temporomandibular (DTM) é uma desordem de etiologia multifatorial em que patologias, deformidades e mau posicionamento do disco da articulação temporomandibular (ATM) estão presentes em até 70% dos casos diagnosticados. Dessa forma, o emprego de conhecimentos e princí- pios da engenharia tecidual para o desenvolvimento de terapias que busquem a regeneração do disco articular pode ser uma opção de tratamento futuro. Células- -tronco mesenquimais (MSC) são frequentemente empregadas, apresentando a capacidade de se diferenciar em condrócitos e depositar tecido semelhante ao da ATM. Estudos têm apontado que essas células podem apresentar melhor regeneração que células removidas da própria ATM lesionada, que apresentam uma menor deposição de matriz extracelular. Abordagens para reconstrução têm empregado, principalmente, scaffolds sintéticos, como polímeros e hidrogéis, assim como scaffolds naturais de origem colágena. Esses materiais têm possibilitado a proliferação celular e a deposição de matriz extracelular. Técnicas de descelularização com diversos solventes orgânicos têm apresentado a capacidade de não desenvolver resposta imune, sendo possível sua utilização. A utilização de fatores de crescimento parece contribuir significativamente na sinalização e diferenciação celular, incrementando a deposição de tecido cartilaginoso. Considerações finais: apesar de a regeneração tecidual do disco articular se apresentar como uma provável opção de tratamento para os diversos tipos de DTM, a literatura ainda se encontra em fase inicial de investigação, com estudos predominantemente in vitro e in vivo.

13.
Braz Oral Res ; 30(1): e93, 2016 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-27737353

RESUMO

The aim of the present study was to evaluate the expression of transforming growth factor-ß1 (TGF-ß1) and osteonectin (ON) in pulp-like tissues developed by tissue engineering and to compare it with the expression of these proteins in pulps treated with Ca(OH)2 therapy. Tooth slices were obtained from non-carious human third molars under sterile procedures. The residual periodontal and pulp soft tissues were removed. Empty pulp spaces of the tooth slice were filled with sodium chloride particles (250-425 µm). PLLA solubilized in 5% chloroform was applied over the salt particles. The tooth slice/scaffold (TS/S) set was stored overnight and then rinsed thoroughly to wash out the salt. Scaffolds were previously sterilized with ethanol (100-70°) and washed with phosphate-buffered saline (PBS). TS/S was treated with 10% EDTA and seeded with dental pulp stem cells (DPSC). Then, TS/S was implanted into the dorsum of immunodeficient mice for 28 days. Human third molars previously treated with Ca(OH)2 for 90 days were also evaluated. Samples were prepared and submitted to histological and immunohistochemical (with anti-TGF-ß1, 1:100 and anti-ON, 1:350) analyses. After 28 days, TS/S showed morphological characteristics similar to those observed in dental pulp treated with Ca(OH)2. Ca(OH)2-treated pulps showed the usual repaired pulp characteristics. In TS/S, newly formed tissues and pre-dentin was colored, which elucidated the expression of TGF-ß1 and ON. Immunohistochemistry staining of Ca(OH)2-treated pulps showed the same expression patterns. The extracellular matrix displayed a fibrillar pattern under both conditions. Regenerative events in the pulp seem to follow a similar pattern of TGF-ß1 and ON expression as the repair processes.


Assuntos
Hidróxido de Cálcio/farmacologia , Polpa Dentária/efeitos dos fármacos , Osteonectina/análise , Células-Tronco/efeitos dos fármacos , Fator de Crescimento Transformador beta1/análise , Animais , Hidróxido de Cálcio/uso terapêutico , Células Cultivadas , Polpa Dentária/citologia , Dentina/efeitos dos fármacos , Matriz Extracelular/efeitos dos fármacos , Regeneração Tecidual Guiada/métodos , Humanos , Imuno-Histoquímica , Camundongos , Odontoblastos/efeitos dos fármacos , Osteonectina/efeitos dos fármacos , Reprodutibilidade dos Testes , Fatores de Tempo , Engenharia Tecidual/métodos , Tecidos Suporte , Fator de Crescimento Transformador beta1/efeitos dos fármacos
14.
PLoS One ; 11(7): e0155231, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27441840

RESUMO

BACKGROUND: The cathodic polarization seems to be an electrochemical method capable of modifying and coat biomolecules on titanium surfaces, improving the surface activity and promoting better biological responses. OBJECTIVE: The aim of the systematic review is to assess the scientific literature to evaluate the cellular response produced by treatment of titanium surfaces by applying the cathodic polarization technique. DATA, SOURCES, AND SELECTION: The literature search was performed in several databases including PubMed, Web of Science, Scopus, Science Direct, Scielo and EBSCO Host, until June 2016, with no limits used. Eligibility criteria were used and quality assessment was performed following slightly modified ARRIVE and SYRCLE guidelines for cellular studies and animal research. RESULTS: Thirteen studies accomplished the inclusion criteria and were considered in the review. The quality of reporting studies in animal models was low and for the in vitro studies it was high. The in vitro and in vivo results reported that the use of cathodic polarization promoted hydride surfaces, effective deposition, and adhesion of the coated biomolecules. In the experimental groups that used the electrochemical method, cellular viability, proliferation, adhesion, differentiation, or bone growth were better or comparable with the control groups. CONCLUSIONS: The use of the cathodic polarization method to modify titanium surfaces seems to be an interesting method that could produce active layers and consequently enhance cellular response, in vitro and in vivo animal model studies.


Assuntos
Materiais Biocompatíveis/farmacologia , Técnicas Eletroquímicas/métodos , Titânio/farmacologia , Animais , Células/efeitos dos fármacos , Eletrodos , Modelos Animais , Propriedades de Superfície
15.
Braz. oral res. (Online) ; 30(1): e93, 2016. graf
Artigo em Inglês | LILACS-Express | ID: biblio-952019

RESUMO

Abstract The aim of the present study was to evaluate the expression of transforming growth factor-β1 (TGF-β1) and osteonectin (ON) in pulp-like tissues developed by tissue engineering and to compare it with the expression of these proteins in pulps treated with Ca(OH)2 therapy. Tooth slices were obtained from non-carious human third molars under sterile procedures. The residual periodontal and pulp soft tissues were removed. Empty pulp spaces of the tooth slice were filled with sodium chloride particles (250-425 µm). PLLA solubilized in 5% chloroform was applied over the salt particles. The tooth slice/scaffold (TS/S) set was stored overnight and then rinsed thoroughly to wash out the salt. Scaffolds were previously sterilized with ethanol (100-70°) and washed with phosphate-buffered saline (PBS). TS/S was treated with 10% EDTA and seeded with dental pulp stem cells (DPSC). Then, TS/S was implanted into the dorsum of immunodeficient mice for 28 days. Human third molars previously treated with Ca(OH)2 for 90 days were also evaluated. Samples were prepared and submitted to histological and immunohistochemical (with anti-TGF-β1, 1:100 and anti-ON, 1:350) analyses. After 28 days, TS/S showed morphological characteristics similar to those observed in dental pulp treated with Ca(OH)2. Ca(OH)2-treated pulps showed the usual repaired pulp characteristics. In TS/S, newly formed tissues and pre-dentin was colored, which elucidated the expression of TGF-β1 and ON. Immunohistochemistry staining of Ca(OH)2-treated pulps showed the same expression patterns. The extracellular matrix displayed a fibrillar pattern under both conditions. Regenerative events in the pulp seem to follow a similar pattern of TGF-β1 and ON expression as the repair processes.

16.
Braz. dent. j ; 26(6): 566-571, Nov.-Dec. 2015. tab
Artigo em Inglês | LILACS | ID: lil-769567

RESUMO

Studies based on dentists' clinical practice possess vital relevance to understand factors leading the clinicians to choose by a specific technique over another. This study investigated which clinical conduct therapies are adopted by dentists in front of deep caries. Was evaluated how the place of work, post-graduate training and years since complete graduation influenced their decisions. A cross-sectional study was performed using a self-applied questionnaire with dentists (n=276) in Southern Brazil. Information regarding post-graduation training (specialization, master's or PhD), clinical experience (years since completing graduation) and place of work were investigated. The information regarding pulp vital therapies (materials for direct pulp capping; techniques for caries removal in deep cavities and strategies for indirect pulp capping) were collected by specific questions. Data were submitted to descriptive analysis and Exact Fischer Test. Response rate was 68% (187). The majority of dentists selected the calcium hydroxide (CH) as first material for direct (86.3%) and indirect (80.3%) pulp protection. Partial caries removal was reported by 61.9% of dentists. Less experienced clinical dentists choose partial caries removal more frequently (p=0.009), if compared with dentists graduated 10 years and up ago. The use of MTA was more common among professionals working at academic environment. Besides, MTA was not mentioned by professionals working exclusively in the public health service (p=0.003). In conclusion, the time since graduation influenced the clinical conduct related to caries removal. The choice of liner materials was influenced by dentists' workplace.


Resumo Estudos baseados na prática clínica de dentistas possuem relevância vital para o entendimento dos fatores que influenciam os clínicos na escolha de uma técnica específica em detrimento de outra. Este estudo investigou quais condutas clínicas são adotadas por dentistas frente a lesões profundas de cárie. Foi avaliado como o local de trabalho, curso de pós-graduação e tempo desde a graduação influenciaram suas decisões clínicas. Foi conduzido um estudo transversal utilizando questionários auto- aplicados à dentistas no sul do Brasil (n=276). Informações relacionadas a educação continuada (especialização, mestrado ou doutorado), experiência clínica (anos desde a graduação) e local de trabalho foram investigadas. As informações sobre terapias vitais da polpa (materiais para capeamento pulpar direto, técnicas para remoção de carie em cavidades profundas e estratégias para capeamento pulpar indireto) foram coletadas por meio de perguntas específicas. Os dados foram submetidos à análise descritiva e teste exato de Fisher. A taxa de resposta foi de 68% (187). A maioria dos dentistas selecionam o hidróxido de cálcio (HC) como primeiro material para proteção pulpar direta (86,3%) e indireta (80,3%). Remoção parcial de tecido cariado foi relatado por 61,9% dos clínicos. Dentistas com menor experiência clínica optaram pela remoção parcial de tecido cariado com mais frequência (p=0,009), quando comparados a dentistas graduados há mais de 10 anos. O uso do MTA foi mais comum entre os profissionais que trabalham em ambiente acadêmico. Além disso, o MTA não foi mencionado pelos profissionais que trabalham exclusivamente no serviço público de saúde (p=0,003). O tempo desde a graduação influenciou a conduta clínica relacionada à remoção de cárie. A escolha de materiais para proteção pulpar foi influenciada pelo local de trabalho dos dentistas.


Assuntos
Humanos , Masculino , Feminino , Serviços de Saúde Bucal , Polpa Dentária , Brasil , Inquéritos e Questionários
17.
RFO UPF ; 20(3): 325-333, set./dez.2015.
Artigo em Português | LILACS-Express | ID: biblio-275

RESUMO

Os Centros de Especialidades Odontológicas (CEOs) são estabelecimentos que servem de referência para unidades básicas de saúde, devem estar vinculados ao Cadastro Nacional de Estabelecimentos de Saúde (CNES) e ofertar procedimentos odontológicos especializados à população. Objetivo: este estudo tem como objetivo descrever a produção odontológica especializada nos municípios do Rio Grande do Sul com CEOs, com base nos dados do Sistema de Informações Ambulatoriais do Sistema Único de Saúde (SIA SUS). Materiais e método: foi conduzido um estudo do tipo longitudinal retrospectivo com utilização de dados secundários do SIA SUS. Realizou-se uma consulta no CNES dos CEOs cadastrados em municípios gaúchos. A produção odontoló- gica dessas cidades foi então pesquisada diretamente no banco de dados do Departamento de Informática do SUS (Data SUS) e posteriormente tabulada. A partir disso, criou-se uma série histórica para cada cidade. A produção mínima mensal variou de acordo com as modalidades de CEOs (Portaria Interministerial nº 1.464 de 2011) e o número de CEOs por município. Resultados: procedimentos da área de periodontia foram os predominantes (46,2%), seguidos de cirurgia oral menor (41,3%) e endodontia (12,5%). Quanto ao cumprimento das metas, Pelotas foi o município com melhor desempenho (91%), seguido de Caxias do Sul (90%) e Canoas (86%). Em contraponto, treze municípios atingiram apenas 25% ou menos das metas estabelecidas. Conclusão: há uma vasta diferença no cumprimento ou registro dos procedimentos odontológicos especializados entre os municípios com CEOs do RS.

18.
Braz Dent J ; 26(6): 566-71, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26963197

RESUMO

Studies based on dentists' clinical practice possess vital relevance to understand factors leading the clinicians to choose by a specific technique over another. This study investigated which clinical conduct therapies are adopted by dentists in front of deep caries. Was evaluated how the place of work, post-graduate training and years since complete graduation influenced their decisions. A cross-sectional study was performed using a self-applied questionnaire with dentists (n=276) in Southern Brazil. Information regarding post-graduation training (specialization, master's or PhD), clinical experience (years since completing graduation) and place of work were investigated. The information regarding pulp vital therapies (materials for direct pulp capping; techniques for caries removal in deep cavities and strategies for indirect pulp capping) were collected by specific questions. Data were submitted to descriptive analysis and Exact Fischer Test. Response rate was 68% (187). The majority of dentists selected the calcium hydroxide (CH) as first material for direct (86.3%) and indirect (80.3%) pulp protection. Partial caries removal was reported by 61.9% of dentists. Less experienced clinical dentists choose partial caries removal more frequently (p=0.009), if compared with dentists graduated 10 years and up ago. The use of MTA was more common among professionals working at academic environment. Besides, MTA was not mentioned by professionals working exclusively in the public health service (p=0.003). In conclusion, the time since graduation influenced the clinical conduct related to caries removal. The choice of liner materials was influenced by dentists' workplace.


Assuntos
Serviços de Saúde Bucal , Polpa Dentária , Brasil , Feminino , Humanos , Masculino , Inquéritos e Questionários
19.
Braz. dent. j ; 24(5): 527-531, Sep-Oct/2013. tab
Artigo em Inglês | LILACS | ID: lil-697641

RESUMO

This study evaluated the preferences of general dentists regarding vital and nonvital tooth bleaching therapies and investigated whether the time of clinical practice and post-graduate training influence these options. A cross-sectional study was conducted using a questionnaire with closed questions applied to dentists (n=276) of a mid-sized city of the southern Brazil (Pelotas, RS). Information was collected regarding sociodemographic variables, level of specialization and time since graduation. In addition, options regarding bleaching therapies including the first choice of material, technique and clinical practice for vital and nonvital tooth bleaching therapies were included. Data were submitted to descriptive analysis and the associations were evaluated using chi-square and Fisher exact tests (α=0.05). The response rate was 68% (n=187). At-home bleaching therapy was broadly preferred (78.1%) over in-office (21.9%) bleaching. For at-home bleaching, most dentists answered to use 10% carbamide peroxide (CP) (40.2%) and >30% hydrogen peroxide (HP) (31.7%) for nonvital therapies. The majority of dentists with post-graduation training preferred at-home bleaching techniques (p=0.003). At-home bleaching therapy was also more indicated by younger dentists. No association was found between the choice for nonvital bleaching therapies and time since graduation (p=0.532) or continuous education (p=0.083). In conclusion, at-home bleaching was preferred over in-office therapies; 10% CP and >30% HP were chosen as first option agents to treat discolored vital and nonvital teeth, respectively. The time in clinical practice and the level of specialization affected dentists' choices only for vital tooth bleaching treatment.


Este estudo avaliou as preferências de cirurgiões-dentistas sobre o clareamento de dentes vitais e não-vitais. Também investigou a possível relação entre o grau de formação profissional e as decisões clínicas tomadas por estes profissionais. Para isso, realizamos um estudo transversal. Cirurgiões-dentistas (n=276) de uma cidade de médio porte do sul do Brasil (Pelotas, RS) receberam um questionário contendo perguntas sobre informação sócio-demográfica, nível de especialização e ano de graduação. Adicionalmente, foram realizadas perguntas sobre o material de primeira escolha, técnicas e práticas clínicas relacionadas a clareamento de dentes vitais e não vitais. Os dados obtidos foram submetidos à análise descritiva e as possíveis associações entre variáveis foram avaliadas utilizando o teste Qui-Quadrado e teste Exato de Fisher (p<0,05). A taxa de resposta foi de 68% (187). A técnica de clareamento caseiro supervisionada foi preferida (78,1%) em detrimento da técnica em consultório (21,9%). Os dentistas escolheram preferentemente o peróxido de carbamida (PC) a 10% para clareamento vital caseiro (40,2%) e o peróxido de hidrogênio (PH) >30% para o clareamento de dentes despolpados (31,7%). A maioria dos dentistas que realizaram cursos de pós-graduação preferiram o clareamento caseiro (p=0,003). Além disso, o clareamento caseiro foi mais indicado pelos dentistas mais jovens. Não foi encontrada relação entre as escolhas para clareamento de dentes despolpados e ano de graduação (p=0,532) ou grau de formação (p=0,083). O clareamento vital caseiro foi escolhido em detrimento das técnicas de consultório; CP a 10% e HP > 30% foram os agentes de escolha para o tratamento de dentes vitais e não vitais escurecidos, respectivamente. O tempo de prática clínica e o nível de especialização foram associados com as escolhas dos dentistas somente para clareamento de dentes vitais.


Assuntos
Feminino , Humanos , Masculino , Odontólogos , Padrões de Prática Odontológica , Clareamento Dental , Brasil , Inquéritos e Questionários
20.
Braz Dent J ; 24(5): 527-31, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24474298

RESUMO

This study evaluated the preferences of general dentists regarding vital and nonvital tooth bleaching therapies and investigated whether the time of clinical practice and post-graduate training influence these options. A cross-sectional study was conducted using a questionnaire with closed questions applied to dentists (n=276) of a mid-sized city of the southern Brazil (Pelotas, RS). Information was collected regarding sociodemographic variables, level of specialization and time since graduation. In addition, options regarding bleaching therapies including the first choice of material, technique and clinical practice for vital and nonvital tooth bleaching therapies were included. Data were submitted to descriptive analysis and the associations were evaluated using chi-square and Fisher exact tests (α=0.05). The response rate was 68% (n=187). At-home bleaching therapy was broadly preferred (78.1%) over in-office (21.9%) bleaching. For at-home bleaching, most dentists answered to use 10% carbamide peroxide (CP) (40.2%) and >30% hydrogen peroxide (HP) (31.7%) for nonvital therapies. The majority of dentists with post-graduation training preferred at-home bleaching techniques (p=0.003). At-home bleaching therapy was also more indicated by younger dentists. No association was found between the choice for nonvital bleaching therapies and time since graduation (p=0.532) or continuous education (p=0.083). In conclusion, at-home bleaching was preferred over in-office therapies; 10% CP and >30% HP were chosen as first option agents to treat discolored vital and nonvital teeth, respectively. The time in clinical practice and the level of specialization affected dentists' choices only for vital tooth bleaching treatment.


Assuntos
Odontólogos , Padrões de Prática Odontológica , Clareamento Dental , Brasil , Feminino , Humanos , Masculino , Inquéritos e Questionários
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