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1.
Braz. j. biol ; 82: e238763, 2022. tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1180734

RESUMO

Abstract We report the discovery that the earwig predator Doru luteipes (Scudder, 1876) (Dermaptera: Forficulidae) feed on Puccinia polysora Underw uredospore, the causal agent of Southern Rust of Corn (SRC), which is a primary disease affecting the maize crop in Brazil. We performed experiments in laboratory and greenhouse to test the effect of D. luteipes (1st/2nd and 3rd/4th instars, and adults) fungivory on the P. polysora uredospore concentration. All trials showed a significant reduction of the initial concentration of uredospore. There was a reduction in uredospore concentration with increase in number of D. luteipes feeding on them. We also tested the uredospore consumption by quantifying its percentage in the feces of D. luteipes. Nymphs of the 2nd, 4th instar and adults fed 88%, 85%, and 83.8% of the uredospore, respectively. For nymphs of the 3rd instar, the percentage of uredospore consumption (75.6%) was statistically significant compared with the other groups. In greenhouse experiment, at twenty-eight days after plant inoculation with 9.9 x 104 uredospores, the percentage of uredospore consumption was 81.7%. Our results confirmed the fungivory of D. luteipes on P. polysora uredospore. This is the first report of D. luteipes fungivory, which may play an important role in the biological control of P. polysora in corn.


Resumo Relatamos a descoberta de que o predador Doru luteipes (Scudder, 1876) (Dermaptera: Forficulidae) se alimenta de uredosporos de Puccinia polysora Underw, o agente causal da ferrugem polisora que é uma doença primária que afeta a cultura do milho no Brasil. Realizamos experimentos em laboratório e em casa de vegetação para testar o efeito da fungivoria de D. luteipes (1º/2º e 3º/4º iìstares e adultos) sobre a concentração de uredosporos de P. polysora. Todos os ensaios mostraram uma redução significativa da concentração inicial de uredosporos. Houve uma redução na concentração de uredosporos com o aumento do número de D. luteipes alimentando-se deles. Também testamos o consumo de uredosporos quantificando sua porcentagem nas fezes de D. luteipes. Ninfas do 2º e 4º ínstar, assim como adultos, alimentaram-se de 88%, 85% e 83,8% dos uredosporos, respectivamente. Para ninfas do 3º ínstar, a porcentagem de consumo de uredosporos (75,6%) foi estatisticamente significativo em comparação com os outros grupos. No experimento em casa de vegetação, aos 28 dias após a inoculação das plantas com 9,9 x 104 uredosporos, a porcentagem de consumo de uredosporos foi de 81,7%. Nossos resultados confirmaram a fungivoria de D. luteipes em uredosporos de P. polysora. Este é o primeiro relato de fungivoria de D. luteipes, que pode ter papel importante no controle biológico de P. polysora em milho.

2.
Braz J Biol ; 82: e238763, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33825760

RESUMO

We report the discovery that the earwig predator Doru luteipes (Scudder, 1876) (Dermaptera: Forficulidae) feed on Puccinia polysora Underw uredospore, the causal agent of Southern Rust of Corn (SRC), which is a primary disease affecting the maize crop in Brazil. We performed experiments in laboratory and greenhouse to test the effect of D. luteipes (1st/2nd and 3rd/4th instars, and adults) fungivory on the P. polysora uredospore concentration. All trials showed a significant reduction of the initial concentration of uredospore. There was a reduction in uredospore concentration with increase in number of D. luteipes feeding on them. We also tested the uredospore consumption by quantifying its percentage in the feces of D. luteipes. Nymphs of the 2nd, 4th instar and adults fed 88%, 85%, and 83.8% of the uredospore, respectively. For nymphs of the 3rd instar, the percentage of uredospore consumption (75.6%) was statistically significant compared with the other groups. In greenhouse experiment, at twenty-eight days after plant inoculation with 9.9 x 104 uredospores, the percentage of uredospore consumption was 81.7%. Our results confirmed the fungivory of D. luteipes on P. polysora uredospore. This is the first report of D. luteipes fungivory, which may play an important role in the biological control of P. polysora in corn.


Assuntos
Zea mays , Animais , Brasil , Ninfa
3.
Arq Bras Cardiol ; 102(3 Suppl 1): 1-61, 2014 03.
Artigo em Português | MEDLINE | ID: mdl-24862929
4.
Nitric Oxide ; 27(1): 67-71, 2012 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-22580230

RESUMO

Nitric oxide (NO) plays an important role as a leishmanicidal agent in murine macrophages. NO resistant Escherichia coli and Mycobacterium tuberculosis have been associated with poor outcomes of their resulting diseases. NO resistant Leishmania braziliensis has also been identified and exacerbates the clinical course of human leishmaniasis. We report, for the first time, natural resistance of Leishmania chagasi promastigotes to NO. These parasites were isolated from humans and dogs with visceral leishmaniasis. We also demonstrate that this resistance profile was associated with a greater survival capacity and a greater parasite burden in murine macrophages, independent of activation and after activation by IFN-γ and LPS.


Assuntos
Leishmania/efeitos dos fármacos , Leishmaniose Visceral/parasitologia , Óxido Nítrico/farmacologia , Animais , Brasil , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Cães , Resistência a Medicamentos , Humanos , Concentração Inibidora 50 , Leishmaniose Visceral/tratamento farmacológico , Leishmaniose Visceral/veterinária , Estágios do Ciclo de Vida , Macrófagos/parasitologia , Camundongos , Doadores de Óxido Nítrico/farmacologia , Carga Parasitária , S-Nitroso-N-Acetilpenicilamina/farmacologia
5.
Vaccine ; 29 Suppl 4: D65-9, 2011 Dec 30.
Artigo em Inglês | MEDLINE | ID: mdl-22185829

RESUMO

Since 1999, vesicular infections caused by Orthopoxvirus in humans and animals, mainly in dairy cattle, have been identified in 20 municipalities in the Rio de Janeiro state of Brazil. This paper describes studies conducted in counties of the northwestern, middle-Paraíba Valley and southern regions of the Rio de Janeiro state where 77 human, 346 bovine and 78 rodent samples were collected over the past ten years. Laboratory investigations using virus isolation, electron microscopy, molecular biology (PCR) and serological analysis confirmed Orthopoxvirus infections in 77.9% of human, 49.2% of dairy cattle and 17.9% of rodent samples. The characterisation of the Cantagalo/IOC strain reconfirmed that this virus was a vaccinia-like virus. In other regions of the Rio de Janeiro state, vesicular/pustular infections in animals and humans are suspected but these have not yet been confirmed. A continuous surveillance system has been established to monitor these regions in addition to several other states of the Brazilian Federation.


Assuntos
Orthopoxvirus/patogenicidade , Infecções por Poxviridae/epidemiologia , Infecções por Poxviridae/veterinária , Zoonoses/epidemiologia , Animais , Brasil/epidemiologia , Bovinos , Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/virologia , Doenças Transmissíveis Emergentes/epidemiologia , Doenças Transmissíveis Emergentes/virologia , Humanos , Orthopoxvirus/isolamento & purificação , Doenças dos Roedores/epidemiologia , Doenças dos Roedores/virologia , Virologia/métodos , Zoonoses/virologia
6.
Electromyogr Clin Neurophysiol ; 50(2): 107-12, 2010 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-20405786

RESUMO

INTRODUCTION: Orofacial pain and pain in the muscles of mastication are frequent symptoms of temporomandibular disorder. The masseter is the closet masticatory muscle to the surface and has the function of raising and retracting the mandible. This muscle has considerable strength and is one of the main muscles involved in the shredding of food It is therefore of utmost importance in the masticatory cycle and generally the most affected by pain and spasms. OBJECTIVES: The aim of the present study was to analyze the effect of manual therapy with transversal and circular movements on pain and spasm in the masseter muscle, using electromyography and a visual analogue pain scale (VAPS). Eight women who experienced pain upon palpation of the masseter greater than 6 on the VAPS were selected for participation in the study, which employed electromyography and a VAPS for assessment, followed by manual oral physiotherapy and reevaluation. RESULTS: The statistical analysis revealed a reduction in pain, but there was no significant difference in electromyographic activity (p < 0.05). CONCLUSION: It was concluded that massage therapy was effective on pain symptoms, but was not capable of altering the electrical activity of the masseter muscle.


Assuntos
Massagem , Músculo Masseter/fisiopatologia , Manejo da Dor , Trismo/terapia , Adulto , Eletromiografia , Feminino , Humanos , Pessoa de Meia-Idade , Dor/etiologia , Dor/fisiopatologia , Medição da Dor , Estudos Prospectivos , Resultado do Tratamento , Trismo/complicações , Trismo/fisiopatologia , Adulto Jovem
7.
Electromyogr Clin Neurophysiol ; 50(1): 55-60, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-20349559

RESUMO

OBJECTIVES: The proposal of the present study is based on mobilizing the nervous system of the upper hemiplegic limb of stroke victims using the Upper Limb Neurodynamic Test (ULNT 1) and electromyographic analysis of the biceps brachii muscle. METHODS: A pre-experimental study was carried out with pretest and posttest repeated measurements on five stroke victims (4 men and 1 woman; mean age: 52.8 +/- 11 years), with Grade 1 and + 1 spasticity, according to the modified Ashworth scale. Electromyographic analysis of the biceps brachii muscle was performed with the elbow flexed at 90 degrees, following by complete extension. RESULTS: The neurodynamic test led to a decrease in electromyographic activity in the biceps brachii muscle (p < or = 0.05). CONCLUSION: Neural mobilization was efficient in reducing myoelectric activity in the biceps brachii muscle in patients with stroke and may used by physiotherapists as an efficient method for treating patients with this pathology.


Assuntos
Contração Muscular , Espasticidade Muscular/fisiopatologia , Músculo Esquelético/inervação , Músculo Esquelético/fisiopatologia , Modalidades de Fisioterapia , Acidente Vascular Cerebral/fisiopatologia , Eletromiografia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Espasticidade Muscular/etiologia , Espasticidade Muscular/reabilitação , Acidente Vascular Cerebral/complicações , Reabilitação do Acidente Vascular Cerebral , Resultado do Tratamento
9.
Arq Bras Cardiol ; 76(4): 279-84, 2001 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-11323731

RESUMO

OBJECTIVE: The evaluation, by exercise stress testing, of the cardiorespiratory effects of pyridostigmine (PYR), a reversible acetylcholinesterase inhibitor. METHODS: A double-blind, randomized, cross-over, placebo-controlled comparison of hemodynamic and ventilation variables of 10 healthy subjects who underwent three exercise stress tests (the first for adaptation and determination of tolerance to exercise, the other two after administration of placebo or 45mg of PYR). RESULTS: Heart rate at rest was: 68+/-3 vs 68+/-3bpm before and after placebo, respectively (P=0.38); 70+/-2 vs 59+/-2bpm, before and after pyridostigmine, respectively (P<0.01). During exercise, relative to placebo: a significantly lower heart rate after PYR at, respectively, 20% (P=0.02), 40% (P=0.03), 80% (P=0.05) and 100% (P=0.02) of peak effort was observed. No significant differences were observed in arterial blood pressure, oxygen consumption at submaximal and maximal effort, exercise duration, respiratory ratio, CO2 production, ventilation threshold, minute ventilation, and oxygen pulse. CONCLUSION: Pyridostigmine, at a dose of 45mg, decreases heart rate at rest and during exercise, with minimal side effects and without interfering with exercise tolerance and ventilation variables.


Assuntos
Pressão Sanguínea/efeitos dos fármacos , Inibidores da Colinesterase/farmacologia , Teste de Esforço/efeitos dos fármacos , Frequência Cardíaca/efeitos dos fármacos , Brometo de Piridostigmina/farmacologia , Adulto , Estudos Cross-Over , Método Duplo-Cego , Feminino , Humanos , Masculino , Consumo de Oxigênio/efeitos dos fármacos
13.
Rev. SOCERJ ; 7(1): 14-8, jan.-mar. 1994. tab, graf
Artigo em Português | LILACS | ID: lil-165703

RESUMO

Foram estudados ao teste ergométrico (TE) 160 pacientes pós infarto do mioárdio, dos quais 46 (29 por cento) submeteram-se a TE e pré e pós programa de atividade física näo supervisionada, aplicando-se protocolo de Naughton (54 por cento) ou Bruce (46 por cento) em funçäo da relaçäo temporal com o evento agudo. Quarenta e três (93 por cento) eram masculinos, média de idade 57 +/- 11 anos. Os dados do segundo teste realizado 18 +/- 9 meses após o primeiro, demonstraram aumento da tolerância ao exercio (+25 por cento) (P<0,001), reduçäo da frequência cardíaca sumáxima em 7 por cento (p=0,001), sem diferença significativa da frequência cardíaca máxima e da medicaçäo em uso quando da realizaçäo dos testes. Houve reduçäo do peso corporal, tabagismo, dislipidemia e sedentarismo. Näo ocorreram eventos cardíacos durante o período de seguimento do estudo. Conclui-se que a utilizaçäo de Testes Ergométricos sequenciais foi de extrema valia para demonstrar a melhora significativa da performance e da evidênci e isquemia neste grupo de pacientes em programa de reabilitaçäo cardíaca.


Assuntos
Teste de Esforço , Infarto do Miocárdio/reabilitação
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