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1.
Med Sci Monit ; 27: e930457, 2021 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-34489390

RESUMO

BACKGROUND We aimed to evaluate the value of prophylactic extended-field intensity-modulated radiation therapy (IMRT) in the treatment of locally advanced cervical cancer with multiple pelvic lymph node metastases (≥2) and negative common iliac and paraaortic lymph nodes. MATERIAL AND METHODS Thirty-four patient with newly diagnosed cervical cancer (IB1-IVA) and multiple pelvic lymph node metastases (≥2) confirmed by computed tomography and magnetic resonance imaging were randomly divided into an extended-field group (17 patients) and a pelvic-field group (17 patients). In the extended-field group, we added the drainage area of paraaortic lymph nodes on the pelvic field. The pelvic field was administered Dt 45.0 to 50.4 Gy, while the drainage area of paraaortic lymph nodes was administered Dt 40.0 to 45.0 Gy. Both groups were given Irl92 intracavitary radiotherapy after 3 weeks of external irradiation. The total dose of point A was 25.0 to 30.0 Gy, fractional 6.0 to 7.0 Gy. All patients had concurrent platinum-based chemotherapy once weekly until the end of radiotherapy. RESULTS No paraaortic lymph node metastasis was found in the extended-field group (P=0.0184), and disease-free survival (DFS) was prolonged (P=0.0286). Adverse effects in patients with III-IV degree myelosuppression were increased in the extended-field group (P=0.0324). However, all patients recovered after symptomatic treatment. CONCLUSIONS Prophylactic extended-field IMRT with chemotherapy reduced the metastasis rate of paraaortic lymph nodes and prolonged the DFS in patients with locally advanced cervical cancer and multiple pelvic lymph node metastases (≥2), while the toxic adverse effects were tolerated.

2.
Nat Commun ; 12(1): 4880, 2021 08 12.
Artigo em Inglês | MEDLINE | ID: mdl-34385444

RESUMO

Accurate and imperceptible monitoring of electrophysiological signals is of primary importance for wearable healthcare. Stiff and bulky pregelled electrodes are now commonly used in clinical diagnosis, causing severe discomfort to users for long-time using as well as artifact signals in motion. Here, we report a ~100 nm ultra-thin dry epidermal electrode that is able to conformably adhere to skin and accurately measure electrophysiological signals. It showed low sheet resistance (~24 Ω/sq, 4142 S/cm), high transparency, and mechano-electrical stability. The enhanced optoelectronic performance was due to the synergistic effect between graphene and poly (3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS), which induced a high degree of molecular ordering on PEDOT and charge transfer on graphene by strong π-π interaction. Together with ultra-thin nature, this dry epidermal electrode is able to accurately monitor electrophysiological signals such as facial skin and brain activity with low-motion artifact, enabling human-machine interfacing and long-time mental/physical health monitoring.


Assuntos
Eletrodos , Eletrofisiologia/métodos , Epiderme/fisiologia , Desenho de Equipamento/métodos , Monitorização Fisiológica/métodos , Dispositivos Eletrônicos Vestíveis , Artefatos , Compostos Bicíclicos Heterocíclicos com Pontes/química , Condutividade Elétrica , Eletrofisiologia/instrumentação , Eletrofisiologia/normas , Desenho de Equipamento/normas , Grafite/química , Humanos , Estrutura Molecular , Monitorização Fisiológica/instrumentação , Monitorização Fisiológica/normas , Movimento (Física) , Polímeros/química , Poliestirenos/química , Pele
3.
Orphanet J Rare Dis ; 16(1): 273, 2021 06 12.
Artigo em Inglês | MEDLINE | ID: mdl-34118978

RESUMO

BACKGROUND: The differential diagnosis of diffuse cystic lung disease (DCLD) is a clinical challenge. We wish to analyze the distribution of the etiology of DCLD based on data from a single lymphangioleiomyomatosis (LAM) clinic. METHODS: All DCLD patients at the LAM Clinic of Peking Union Medical College Hospital between January 2006 and December 2019 were analyzed. Information on the demographic, clinical, radiological, and pathological features was collected. RESULTS: A total of 1010 patients with DCLD on CT scan were evaluated. A sum of 711(70.4%) patients were diagnosed with definite or probable LAM. Other diagnoses included Birt-Hogg-Dubé syndrome (46), Sjogren's syndrome (38), pulmonary Langerhans cell histiocytosis (14), lung tumors (3), Castleman disease (2), antineutrophil cytoplasmic antibody-associated vasculitis (2), systemic lupus erythematosus (1), Marfan syndrome (1), amyloidosis (1), congenital cystic adenomatoid malformation of the lung (1), and pleuroparenchymal fibroelastosis (1). In the 38 patients diagnosed with Sjogren's syndrome, 2 were diagnosed with light-chain deposition disease, 2 were diagnosed with amyloidosis and 1 was diagnosed with lymphocytic interstitial pneumonia. One hundred and eighty-nine patients (18.7%) were undiagnosed. Lung biopsy results were available in 27 patients in the undiagnosed DCLD group but did not provide a diagnosis. CONCLUSION: Approximately 70% of DCLD patients in our LAM clinic had LAM. The common differential diagnoses included Birt-Hogg-Dubé syndrome, Sjogren's syndrome, and pulmonary Langerhans cell histiocytosis. Detailed clinical information and laboratory, genetic, and pathological investigations provide correct diagnoses in most patients with DCLD.


Assuntos
Síndrome de Birt-Hogg-Dubé , Histiocitose de Células de Langerhans , Doenças Pulmonares Intersticiais , Pneumopatias , Linfangioleiomiomatose , Diagnóstico Diferencial , Histiocitose de Células de Langerhans/diagnóstico , Humanos , Lipopolissacarídeos , Pneumopatias/diagnóstico , Doenças Pulmonares Intersticiais/diagnóstico , Linfangioleiomiomatose/diagnóstico
4.
Small ; 17(24): e2101114, 2021 06.
Artigo em Inglês | MEDLINE | ID: mdl-34013665

RESUMO

Spatially offset Raman spectroscopy is integrated with a fiber-coupled spatial heterodyne spectrometer to collect Raman spectra from deep within opaque or scattering materials. The method, named spatial heterodyne offset Raman spectroscopy generates a wavenumber-dependent spatial phase shift of the optical signal as a "spectral" image on a charge-coupled device detector. The image can be readily processed from the spatial domain using a single, simple, and "on-the-fly" Fourier transform to generate Raman spectra, in the frequency domain. By collecting all of the spatially offset Raman scattered photons that pass through the microscope's collection objective lens, the methodology gives an improvement in the Raman sensitivity by an order of magnitude. The instrumentation is both mechanically robust and "movement-free," which when coupled with the associated advantages of highly efficient signal collection and ease of data processing, enables rapid interfacial analysis of complex constructs based on established biomaterials models.


Assuntos
Materiais Biocompatíveis , Análise Espectral Raman , Fótons
5.
BMC Pulm Med ; 21(1): 146, 2021 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-33941134

RESUMO

BACKGROUND: Transbronchial lung biopsy (TBLB) in the diagnosis of lymphangioleiomyomatosis (LAM) is not a common approach, although TBLB is often performed in diffuse lung diseases. We aimed to examine the diagnostic value and safety of TBLB in LAM patients based on the data collected in our center. METHODS: We reviewed LAM patients registered in our LAM Clinic from December 8, 2006, to December 31, 2019. All patients with definite or probable diagnosis of LAM who had been examined using TBLB were included. All available pathology slides were reviewed by an experienced LAM pathologist. All complications were reviewed by the medical records and confirmed using telephone interviews. RESULTS: The pathology results of 86 patients (including 74 definite LAM and 12 probable LAM) were available. The positive rate of TBLB in LAM patients was 49/86 (57.0%). The positive rates of SMA, HMB-45, ER, and PR in LAM patients were 97.6%, 93%, 84.6%, and 78.4% respectively. The positive rate of TBLB was 40%, 60% and 60.8% in patients with CT Grade I, Grade II, and Grade III respectively, and the difference was not significant. Patients who had 3-4 or 5-6 biopsied specimens had a higher rate of diagnosis than those with 1-2 biopsied specimens. Four patients (5.6%) reported pneumothorax. No major hemoptysis was reported. CONCLUSIONS: TBLB is a feasible and safe procedure for obtaining a pathological diagnosis of LAM. Taking more than 2 samples during the biopsy procedure increased the rate of diagnosis.

6.
Environ Sci Pollut Res Int ; 28(34): 47463-47473, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-33893585

RESUMO

Fine particulate matter (PM2.5) is an important environmental factor affecting human health. However, most studies on PM2.5 and health have used data from fixed monitoring sites to assess PM2.5 exposure, which may have introduced misleading information on the exposure-response relationship. We aimed to assess the effect of short-term personal PM2.5 exposure on lung function in patients with chronic obstructive pulmonary disease (COPD) and asthma. To achieve this, we conducted a longitudinal panel study among 37 COPD patients and 45 asthma patients from Beijing, China. The COPD group and the asthma group completed 148 and 180 lung function tests, respectively. We found that in COPD patients, for every 10-µg/m3 increase in PM2.5 exposure at lag2, the FEV1, FVC and DLco decreased by -0.014 L (95% CI -0.025, -0.003), -0.025 L (95% CI -0.050, -0.003) and -0.089 mmol/min/kPa (95% CI -0.156, -0.023), respectively. There was also a decrease of -0.023 L/s (95% CI -0.042, -0.003) and -0.017 L/s (95% CI -0.032, -0.002) in MMEF at lag3 and lag03, respectively. In the asthma group, every 10-µg/m3 increase in PM2.5 exposure led to a reduction of -0.012 L (95% CI -0.023, -0.001), -0.042 L (95% CI -0.081, -0.003) and -0.061 L/s (95% CI -0.116, -0.004) in the FEV1, FVC and PEF at lag3, respectively. Our findings suggest that PM2.5 exposure may primarily affect both airway function and lung diffusion function in COPD patients, and airway function in asthma patients.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , Asma , Doença Pulmonar Obstrutiva Crônica , Poluentes Atmosféricos/efeitos adversos , Poluentes Atmosféricos/análise , Poluição do Ar/efeitos adversos , Poluição do Ar/análise , Pequim , China , Exposição Ambiental/análise , Humanos , Pulmão/química , Material Particulado/análise
7.
Chemistry ; 27(24): 7080-7084, 2021 Apr 26.
Artigo em Inglês | MEDLINE | ID: mdl-33769604

RESUMO

Double cross-linked dynamic hydrogels, dynagels, have been prepared through reversible imine bonds and supramolecular interactions, which showed good pH responsiveness, injectability, self-healing property and biocompatibility. With the further encapsulation of heparin, the obtained hydrogels exhibited good anti-bacterial activity and promotion effects for 3D cell culture.


Assuntos
Heparina , Hidrogéis , Técnicas de Cultura de Células
8.
Anal Biochem ; : 114140, 2021 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-33610535

RESUMO

A facile, sensitive and unmodified Hg2+ homogeneous electrochemical sensor based on bisferrocene signal markers and catalytic hairpin self-assembly (CHA) was built on a gold disk electrode. Three hairpin probes were designed, in which thiol was labeled at both ends of the hairpin probe 1(HP1), while bisferrocene, a redox signal marker, was labeled at both ends of the hairpin probe 2(HP2) and hairpin probe 3(HP3). Due to the Hg2+ mediated thymine-Hg (II)-thymine (T-Hg2+-T) structure, when Hg2+ is introduced, the T-Hg2+-T that occurred between the probe DNA and helper DNA could open the hairpin structure of probe DNA and form a rigid DNA triangles structure by CHA. Simultaneously, four bisferrocene signal markers also reached the surface of the electrode and built potential-assisted Au-S self-assembly to achieve signal amplification. Under the optimized condition, the sensor can achieve good electrochemical response Hg2+detection, and the detection limit is as low as 0.6 pM. furthermore, this sensor has high selectivity for Hg2+ detection.

9.
Sci Total Environ ; 756: 144154, 2021 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-33310211

RESUMO

Inert gas is often used in the deoxygenation of microbial electrolysis cells (MECs) to maintain growth and viability of anaerobes. However, the effects of the gas atmosphere on hydrogen production and microbial community of MECs are often neglected. Here, the performances and biofilm microbiomes of MECs pre-sparged with different gases were compared. MECs pre-sparged with argon gas (Ar) yielded more hydrogen (3.73 ± 0.13 mol-H2/mol-acetate) and a higher hydrogen production rate (2.99 ± 0.17 L-H2/L-reactor-day) than MECs pre-sparged with N2 (3.41 ± 0.13 mol-H2/mol-acetate and 2.27 ± 0.28 L-H2/L-reactor-day, respectively). Microbiome analysis indicated that the relative abundance of Geobacter increased from 59.25% to 77.79% when the gas atmosphere in MECs shifted from N2 to Ar. Hydrogen production may have been catalyzed by nitrogenase from Geobacter and photosynthetic bacteria in MECs pre-sparged with Ar. These findings suggested that the gas atmosphere substantially influences the microbiome of anode biofilms and Ar sparging is most effective for enhancing hydrogen production in MECs.


Assuntos
Fontes de Energia Bioelétrica , Eletrólise , Atmosfera , Eletrodos , Gases , Hidrogênio
10.
Talanta ; 223(Pt 1): 121709, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-33303159

RESUMO

In this work, an unmodified homogeneous electrochemical sensor based on electrochemical bonding and catalytic hairpin assembly (CHA) was first constructed for the high sensitivity detection of Hg2+. Herein, tetraferrocene, a synthesized compound, was used as a signal marker that modified both ends of the hairpin probe to amplify the electrochemical signal. The interaction of T-Hg2+-T could induce the catalytic self-assembly of hairpins by means of auxiliary DNA. The rigid DNA triangle that was formed easily reaches the electrode and induced Au-S self-assembly assisted by potential, allowing tetraferrocene to reach the electrode surface and generate a sensitive electrochemical signal. CHA and tetraferrocene signal markers accomplished dual signal amplification, and the limit of detection was 0.12 pM. Differential pulse voltammetry experiments in the presence of tetraferrocene redox indicator show that the linear response range of electrochemical biosensors to mercury ions is 0.2-2000 pM, This technology offers good selectivity and high recognition efficiency for the detection of mercury ions and has broad application prospects in actual sample detection.


Assuntos
Técnicas Biossensoriais , Mercúrio , DNA , Técnicas Eletroquímicas , Ouro , Limite de Detecção
11.
Sci Total Environ ; 753: 142281, 2021 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-33207445

RESUMO

A novel method for fabricating air-cathodes was developed by assembling an activated carbon (AC) catalyst together with a waterproof breathable membrane (WBM) and stainless steel mesh (SSM) to reduce manufacturing costs of bioelectrochemical systems (BESs). WBMs made of different materials were tested in the assembly, including a hybrid of polypropylene and polyolefin (PPPO), polyethylene (PE), and polyurethane (PU), and compared against poly tetrafluoroethylene (PTFE)-based cathodes. Results showed that the maximum power density of the activated carbon-stainless steel mesh-polyurethane (AC@SSM/PU) assembly was 2.03 W/m2 while that of conventional carbon cloth cathode assembly (Pt@CC/PTFE) was 1.51 W/m2. Compared to conventional cathode fabrication, AC@SSM/PU had a much lower cost and simpler manufacturing process. Illumina Miseq sequencing of 16S rRNA gene amplicons indicated that microbiomes were substantially different between anode and cathode biofilms. There was also a difference in the community composition between different cathode biofilms. The predominant population in the anode biofilms was Geobacter (38-75% relative abundance), while Thauera and Pseudomonas dominated the cathode biofilms. The results demonstrated that different types of air-cathodes influenced the microbial community assembly on the electrodes.


Assuntos
Fontes de Energia Bioelétrica , Microbiota , Biofilmes , Eletrodos , RNA Ribossômico 16S/genética
12.
Annu Int Conf IEEE Eng Med Biol Soc ; 2020: 3102-3105, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-33018661

RESUMO

Speech recognition based on surface electromyography (sEMG) signals is an important research direction with potential applications in life, work and clinical. The number and placement of sEMG electrodes play a critical role in capturing the underlying sEMG activities and in turn, accurately classifying the speaking tasks. The aim of this work was to investigate the effect of the number of channels in speech recognition based on high-density (HD) sEMG. 8 healthy subjects were recruited to perform 11 English speech tasks with sEMG signals were detected from 120 electrodes covering almost the whole neck and face. The classification accuracy was evaluated in the context of a linear discriminant analysis (LDA) with different sets of EMG electrodes. By comparing the classification accuracy, the sequential forward search (SFS) algorithm was adopted to figure out the optimal combination of electrodes which realized the highest classification level. The results showed that smaller number of channels obtained by the SFS method could achieve the classification accuracy of 80%, and another few electrodes were needed to record detail information to achieve the classification accuracy of 85%, 90% and 95%. The numbers were rather smaller than 120. Considering the computation time and reliable accuracy, it is concluded that the SFS method might be helpful for standardizing the number and position of electrodes in speech recognition.


Assuntos
Percepção da Fala , Fala , Eletromiografia , Humanos , Movimento , Processamento de Sinais Assistido por Computador
13.
Annu Int Conf IEEE Eng Med Biol Soc ; 2020: 3469-3472, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-33018750

RESUMO

Choices and decisions involve a series of complex cognitive processes, and the time-frequency analysis of electroencephalogram (EEG) signals can help understand the brain activities in different cognitive tasks. In this study, a decision-making cognitive task of rock-paper-scissors was designed, and the complex decision-making task was divided into three stages (decision planning, confirmation, and feedback). 64 channels of EEG signals were simultaneously recorded using the Neuroscan QuikCap system during the whole task. The average spectral power and phase synchronization values of each frequency band (delta, theta, alpha and beta band) were extracted and compared within and across different stages. The results showed the desire to win or not to lose within the first stage might be accompanied by the increase of alpha and theta components. In the second stage, the spectral power inhibition of alpha wave and phase synchronization increase of delta wave indicated that subjects would improve their attentions when they confirmed their choice. In the third stage, the theta increased effect and alpha decreased effect were related to different feedback. This pilot study suggests that the time-frequency analysis of EEG signals could be a great tool to visualize the brain activities in response to different cognitive tasks.


Assuntos
Cognição , Eletroencefalografia , Tomada de Decisões , Humanos , Projetos Piloto
14.
Annu Int Conf IEEE Eng Med Biol Soc ; 2020: 3662-3665, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-33018795

RESUMO

Bell's palsy impairs the symmetry of facial appearance and movement. Detailed examination of facial muscle activities should be important for the diagnosis and treatment of the Bell's palsy. In this study, facial muscle activities in normal and Bell's palsy conditions were measured using a high-density (HD) electromyography (EMG) technique. The EMG signals during active tasks (four different facial expressions) and resting task were collected with a HD array of EMG electrodes from forehead and cheek muscles. To visualize facial EMG activities, the EMG maps were reconstructed from the HD-EMG recordings. The two-dimension (2D) correlation coefficients between right and left sides of facial EMG maps were calculated to evaluate the symmetry of facial muscle activities during these tasks. Our experimental results showed that the 2D correlation coefficients during active tasks were different significantly (P<0.01) between the healthy group(n=4) and Bell's palsy group(n=3). These results demonstrated that the synchronism of facial muscle activities during active tasks in healthy subjects is greater than that in the Bell's palsy subjects. This pilot study suggested that HD-EMG would be a potential technique to assess abnormal asymmetric activities of facial muscles for Bell's palsy.


Assuntos
Paralisia de Bell , Paralisia Facial , Paralisia de Bell/diagnóstico , Eletromiografia , Face , Paralisia Facial/diagnóstico , Humanos , Projetos Piloto
15.
Chemistry ; 26(71): 16994-16999, 2020 Dec 18.
Artigo em Inglês | MEDLINE | ID: mdl-32761991

RESUMO

The fabrication of biocompatible adaptive materials with high stiffness and self-healing properties for medical applications is a challenging endeavor. Collagen is a major extracellular matrix component acting as a substrate for cell adhesion and migration. Dynamers are constitutional polymers whose monomeric components are linked through reversible bonds, able to modify their constitution through reversible exchange of their components. In the current work, we demonstrate that the rational combination of collagen and dynameric networks connected with reversible covalent imine bonds is a very important and previously unreported strategy to provide biocompatible membranes with self-healing ability and excellent mechanical strength. The key challenge in the construction of such membranes is the required adaptive interaction between collagen chains and the dynamic cross-linkers, preventing the formation of defects. For example, by varying structure and molecular lengths of the dynamers, the tensile strength of the dynameric membranes reach over 80 MPa, more than 400 % higher than that observed for the reference collagen membrane, and the highest value for break strain found, was 19 %. The self-healing properties were observed when reconnecting two membrane pieces or even from crushed status of the membranes. Moreover, both MTT assay and confocal laser scanning microscopy method demonstrated the good biocompatibility of the collagen membranes, leaving more than 90 % viability for NIH 3T3 cells after 24 h co-culture.


Assuntos
Materiais Biocompatíveis , Colágeno , Animais , Adesão Celular , Proliferação de Células , Camundongos , Polímeros/química
16.
Eur Neuropsychopharmacol ; 39: 99-110, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-32861545

RESUMO

Touch plays a crucial role in affiliative behavior and social communication. The neuropeptide oxytocin is released in response to touch and may act to facilitate the rewarding effects of social touch. However, no studies to date have determined whether oxytocin facilitates behavioral or neural responses to non-socially administered affective touch and possible differential effects of touch valence. In a functional MRI experiment using a randomized placebo-controlled, within-subject design in 40 male subjects we investigated the effects of intranasal oxytocin (24IU) on behavioral and neural responses to positive, neutral and negative valence touch administered to the arm via different types of materials at a frequency aimed to optimally stimulate C-fibers. Results showed that oxytocin significantly increased both the perceived pleasantness of touch and activation of the orbitofrontal cortex independent of touch valence. The effects of OT on touch-evoked orbitofrontal activation were also positively associated with basal oxytocin concentrations in blood. Additionally, anterior insula activity and the functional connectivity between the amygdala and right anterior insula were enhanced only in response to negative valence touch. Overall, the present study provides the first evidence that oxytocin may facilitate the rewarding effects of all types of touch, irrespective of valence.

17.
BMJ Open ; 10(7): e036061, 2020 07 05.
Artigo em Inglês | MEDLINE | ID: mdl-32624473

RESUMO

INTRODUCTION: Preliminary evidence from clinical observations suggests that Tai Chi exercise may offer potential benefits for patients with chronic coronary syndrom (CCS). However, the advantages for CCS patients to practice Tai Chi exercise as rehabilitation have not been rigorously tested and there is a lack of consensus on its benefits. This study aims to develop an innovative Tai Chi Cardiac Rehabilitation Program (TCCRP) for CCS patients and to assess the efficacy, safety and acceptability of the programme. METHODS AND ANALYSIS: We propose to conduct a multicentre randomised controlled clinical trial comprising of 150 participants with CCS. The patients will be randomly assigned in a 1:1 ratio into two groups. The intervention group will participate in a supervised TCCRP held three times a week for 3 months. The control group will receive supervised conventional exercise rehabilitation held three times a week for 3 months. The primary and secondary outcomes will be assessed at baseline, 1 month, 3 months after intervention and after an additional 3-month follow-up period. Primary outcome measures will include a score of 36-Item Short Form Survey and Chinese Perceived Stress Scale. The secondary outcome measures will include body composition, cardiopulmonary exercise test, respiratory muscle function, locomotor skills, echocardiogram, New York Heart Association classification, heart rate recovery time and laboratory examination. Other measures also include Seattle Angina Scale, Pittsburgh Sleep Quality Index, Patient Health Questionnaire-9, Generalized Anxiety Disorder-7 and Berg Balance Scale. All adverse events will be recorded and analysed. ETHICS AND DISSEMINATION: This study conforms to the principles of the Declaration of Helsinki and relevant ethical guidelines. Ethical approval has been obtained from the Ethics Committee of Chinese People's Libration Army General Hospital (approval number: S2019-060-02). Findings from this study will be published and presented at conferences for widespread dissemination of the results. TRIAL REGISTRATION NUMBER: NCT03936504.


Assuntos
Reabilitação Cardíaca/métodos , Doença das Coronárias/reabilitação , Tai Ji , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Angina Pectoris/etiologia , Ansiedade/etiologia , Doença Crônica/psicologia , Doença Crônica/reabilitação , Doença das Coronárias/complicações , Doença das Coronárias/fisiopatologia , Doença das Coronárias/psicologia , Terapia por Exercício , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Multicêntricos como Assunto , Aceitação pelo Paciente de Cuidados de Saúde , Qualidade de Vida , Ensaios Clínicos Controlados Aleatórios como Assunto , Projetos de Pesquisa , Estresse Psicológico/etiologia , Tai Ji/efeitos adversos , Adulto Jovem
18.
Int J Chron Obstruct Pulmon Dis ; 15: 1391-1401, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32606648

RESUMO

Purpose: Circular RNAs (circRNAs) regulate other RNA transcripts by competing for shared microRNAs, which play roles in the pathogenesis of many diseases, including chronic obstructive pulmonary disease (COPD). However, the role of circRNAs in COPD remains unknown. This study aimed to investigate the expression profile and the role of circRNAs in COPD. Patients and Methods: Twenty-one COPD patients and twenty-one normal controls were recruited. Total RNAs were collected from peripheral blood mononuclear cells (PBMCs) of each participant. CircRNAs and protein-coding mRNAs were profiled by microarray and systematically compared between patients with COPD and control subjects. The top differentially expressed circRNAs and mRNAs were validated by quantitative real-time PCR (RT-qPCR). Functional analysis identified pathways relevant to the pathogenesis of COPD. Next, the circRNA target pathway network, the circRNA-miRNA-mRNA network (ceRNA network) and functional ceRNA regulatory modules were constructed. Results: In total, 2132 circRNAs and 2734 protein-coding mRNAs were differentially expressed (|fold change| >1.5 and P-value <0.05) in COPD patients. Six out of nine selected RNAs were confirmed by RT-qPCR validation. Our functional analysis suggested that immune imbalances and inflammatory responses play roles in the pathogenesis of COPD. The ceRNA network highlighted the differentially expressed circRNAs and their related miRNAs and mRNAs in COPD. In the circRNA target pathway network and functional ceRNA regulatory modules, hsa_circRNA_0008672 appeared in the top three KEGG pathways (NOD-like receptor signaling pathway, natural killer cell mediated cytotoxicity and Th17 cell differentiation) and may act as the miRNA sponge regulating the hsa_circRNA_0008672/miR-1265/MAPK1 axis. Conclusion: Our findings demonstrate critical roles of the circRNAs in COPD molecular etiology. The data support a plausible mechanism that circRNAs may be involved in the development of COPD by affecting the immune balance. Moreover, the hsa_circRNA_0008672/miR-1265/MAPK1 axis may contribute to the pathogenesis of COPD, warranting further investigation.


Assuntos
MicroRNAs , Doença Pulmonar Obstrutiva Crônica , Biologia Computacional , Perfilação da Expressão Gênica , Redes Reguladoras de Genes , Humanos , Leucócitos Mononucleares , MicroRNAs/genética , Doença Pulmonar Obstrutiva Crônica/diagnóstico , Doença Pulmonar Obstrutiva Crônica/genética , RNA Circular
19.
Sci Total Environ ; 724: 138321, 2020 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-32408465

RESUMO

Since there is high lignocellulose content in the cotton straw and cow manure mixture (MCC), the appropriate MCC pretreatment is important to promote the anaerobic digestion (AD) hydrolysis. This study mainly explored the effect of potassium ferrate (PF) and peroxymonosulfate (PMS) pretreatments on MCC decomposition. PMS + PF co-treatment showed a higher reduction of total solid and volatile solid than PF pretreatment and PMS pretreatment. Hydrolysis of treated MCC indicated that the PF pretreatment was more effective to the release of organics than the PMS pretreatment and the PMS + PF co-treatment. However, the PMS + PF co-treatment resulted in a higher lignin removal rate (40.4%-50.5%) than the PMS pretreatment (30.8%) and the PF pretreatment (21.4%). The PMS1 + PF2 co-treatment (molar ratio of 1:2) acquired the optimal lignin removal rate and the release of organics among the PMS + PF co-treatment with different dosing ratio. Potential mechanism was that PF reduction products activated PMS to produce free radicals (SO4-, OH), which attacked lignocellulosic components and promoted MCC decomposition. The PMS1 + PF2 co-treatment was deduced to be the optimal pretreatment method when considering MCC decomposition, biodegradability, and mass transfer in the bioreactor.


Assuntos
Compostos de Ferro , Esterco , Animais , Bovinos , Feminino , Peróxidos , Compostos de Potássio
20.
Sci Total Environ ; 733: 139105, 2020 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-32447076

RESUMO

The limitation of hydrolysis and the health risks from pathogenic microorganisms are challenges in the treatment of human waste for volume reduction and nutrient recovery. In this study, potassium ferrate (PF), peroxymonosulfate (PMS), and PF combined with peroxymonosulfate (PMS+ PF) were used as pretreatment or co-treatment methods to enhance nutrient release and control pathogenic microorganisms in human waste. The PF pretreatment was the most effective regarding hydrolysis and organic matter release. The largest difference (D-value) in the soluble chemical oxygen demand (3117.0 mg/L) between the control and the treatment after 120 min was observed for the PF pretreatment, followed by the alkaline (ALK) pretreatment (1525.0 mg/L), the PF + PMS pretreatment (1169.3 mg/L), and the PMS pretreatment (1020.6 mg/L). The PF pre-treated waste exhibited the highest volatile solids reduction of 79.2% after 120 min compared with 15.0% reduction of the untreated waste, as well as the highest polysaccharide release, with a D-value of 198.5 mg/L. All pretreatments exhibited inactivation of pathogenic bacteria and helminths eggs; however, the PF pretreatment was the most efficient method to suppress pathogenic micrograms, with a 3.5 log (N/N0) decrease in the number of total coliforms. The PF pretreatment and PMS + PF co-treatment both exhibited the good performance regarding nitrogen release, including soluble protein and ammonium. The maximum D-value of the total soluble nitrogen was 372.8 mg/L for the PF + PMS co-treatment. The maximum D-value of soluble protein was 156.2 mg/L for the ALK pretreatment. The results indicated that the PF pretreatment was the most effective method for disintegrating human waste, thus providing a new method for safe and rapid reduction of human waste, as well as nutrient release.


Assuntos
Nutrientes , Esgotos , Análise da Demanda Biológica de Oxigênio , Humanos , Hidrólise , Nitrogênio
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