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1.
J Environ Sci (China) ; 124: 688-698, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36182174

RESUMO

It has been documented that organic contaminants can be degraded by hydroxyl radicals (•OH) produced by the activation of H2O2 by Fe(II)-bearing clay. However, the interfacial electron transfer reactions between structural Fe(II) and H2O2 for •OH generation and its effects on contaminant remediation are unclear. In this study, we first investigated the relation between •OH generation sites and sulfamethoxazole (SMX) degradation by activating H2O2 using nontronite with different reduction extents. SMX (5.2-16.9 µmol/L) degradation first increased and then decreased with an increase in the reduction extent of nontronite from 22% to 62%, while the •OH production increased continually. Passivization treatment of edge sites and structural variation results revealed that interfacial electron transfer reactions between Fe(II) and H2O2 occur at both the edge and basal plane. The enhancement on basal plane interfacial electron transfer reactions in a high reduction extent rNAu-2 leads to the enhancement on utilization efficiencies of structural Fe(II) and H2O2 for •OH generation. However, the •OH produced at the basal planes is less efficient in oxidizing SMX than that of at edge sites. Oxidation of SMX could be sustainable in the H2O2/rNAu-2 system through chemically reduction. The results of this study show the importance role of •OH generation sites on antibiotic degradation and provide guidance and potential strategies for antibiotic degradation by Fe(II)-bearing clay minerals in H2O2-based treatments.


Assuntos
Peróxido de Hidrogênio , Sulfametoxazol , Antibacterianos , Argila , Elétrons , Compostos Ferrosos , Minerais/química , Oxirredução , Sulfametoxazol/química
2.
Bioact Mater ; 19: 237-250, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-35510176

RESUMO

The limited clinical response and serious side effect have been challenging in cancer immunotherapy resulting from immunosuppressive tumor microenvironment (TME) and inferior drug targeting. Herein, an active targeting TME nanoplatform capable of revising the immunosuppressive TME microenvironment is designed. Briefly, gold nanorods (GNRs) are covered with silica dioxide (SiO2) and then coated manganese dioxide (MnO2) to obtain GNRs@SiO2@MnO2 (GSM). Myeloid-derived suppressor cells (MDSCs) membrane is further camouflaged on the surface of GSM to obtain GNRs@SiO2@MnO2@MDSCs (GSMM). In this system, GSMM inherits active targeting TME capacity of MDSCs. The localized surface plasmon resonance of GNRs is developed in near-infrared II window by MnO2 layer coating, realizing NIR-II window photothermal imaging and photoacoustic imaging of GSMM. Based on the release of Mn2+ in acidic TME, GSMM can be also used for magnetic resonance imaging. In cancer cells, Mn2+ catalyzes H2O2 into ·OH for (chemodynamic therapy) CDT leading to activate cGAS-STING, but also directly acts on STING inducing secretion of type I interferons, pro-inflammatory cytokines and chemokines. Additionally, photothermal therapy and CDT-mediated immunogenic cell death of tumor cells can further enhance anti-tumor immunity via exposure of CRT, HMGB1 and ATP. In summary, our nanoplatform realizes multimodal cancer imaging and dual immunotherapy.

3.
Phytomedicine ; 108: 154518, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36403513

RESUMO

BACKGROUND: Chios mastic gum (CMG) is a traditional Greek medicine used to treat a variety of gastrointestinal disorders, including inflammatory bowel disease (IBD). However, the bioactive compounds of CMG and the mechanisms of action for controlling of IBD remain unknown. PURPOSE: Masticadienonic acid (MDA) is one of the most abundant constituents isolated from CMG. This study aims to investigate the potential effects and underlying mechanisms of MDA in the pathogenesis of colitis. METHODS: The effects of MDA were evaluated using a dextran sulphate sodium (DSS)-induced acute colitis mouse model. The body and spleen weight and colon length and weight were measured and the clinical symptoms were analysed. Blood samples were collected to analyse the level of serum inflammatory markers. Colon tissues were processed for histopathological examination, evaluation of the epithelial barrier function, and investigation of the probable mechanisms of action. The gut microbiota composition was also studied to determine the mechanism for the beneficial effects of MDA on IBD. RESULTS: MDA could ameliorate the severity of IBD by increasing the body weight and colon length, reducing spleen weight, disease activity index, and histological score. MDA treatments reduce the release of serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1ß), and interleukin 6 (IL-6) via inhibiting the MAPK and NF-κB signalling pathways. MDA supplementation could also improve the intestinal barrier function by activating the NF-E2-related factor-2 (Nrf2) signalling pathway and restoring the expression of tight junction proteins zonula occludens-1 (ZO-1) and occludin. In addition, MDA administration modulates the gut microbiota composition in DSS-induced colitis mice. CONCLUSION: The results indicate that MDA attenuated experimental colitis by restoring intestinal barrier integrity, reducing inflammation, and modulating the gut microbiota. The present study provides novel insights into CMG-mediated remission of IBD and may facilitate the development of preventive and therapeutic strategies for IBD.

4.
Sci Total Environ ; 857(Pt 2): 159459, 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36252670

RESUMO

Microbial community structure plays a crucial part in soil organic carbon (SOC) decomposition and variation of rhizosphere priming effects (RPEs) during plant growth. However, it is still uncertain how bacterial community structure regulates RPEs in soil and how RPE patterns respond to plant growth. Therefore, we conducted an experiment to examine the RPE response to plant growth and nitrogen (N) addition (0 (N0), 150 (N150), and 300 (N300) kg N ha-1) using the 13C natural abundance method in a C3 soil (paddy soil) - C4 plant (maize, Zea mays L.) system; we then explored the underlying biotic mechanisms using 16S rRNA sequencing techniques. Networks were constructed to identify keystone taxa and to analyze the correlations between network functional modules of bacterial community and C decomposition. The results indicated that negative and positive RPEs occurred on Day 30 and Day 75 after maize planting, respectively. Bacterial community structure significantly changed and tended to shift from r-strategists toward K-strategists with changing labile C: N stoichiometry and soil pH during plant growth stages. The different network modules of bacterial community were aggregated in response to RPE pattern variation. Caulobacteraceae, Bacillus, and Chitinophagaceae were keystone taxa on Day 30, while Gemmatimonas, Candidatus Koribacter, and Xanthobacteraceae were keystone taxa on Day 75. Moreover, keystone taxa with different C utilization strategies were significantly different between the two growth stages and related closely to different RPE patterns. This study provides deeper insights into the network structure of bacterial communities corresponding to RPE patterns and emphasizes the significance of keystone taxa in RPE variation.


Assuntos
Microbiota , Rizosfera , Solo/química , Carbono , Microbiologia do Solo , RNA Ribossômico 16S , Plantas , Bactérias , Zea mays
5.
Sci Total Environ ; : 160307, 2022 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-36403824

RESUMO

Simultaneous mitigation of Arsenic (As) and Cadmium (Cd) in rice grains is hardly achieved with conventional soil treatments due to their opposite chemical behaviors in paddy soils. This study evaluates the effectiveness of a novel foliar inhibitor with germanium (Ge) -modified zeolitic imidazolate framework (ZIF-8@Ge-132) in cooperative mitigation of As and Cd in rice grains in a As and Cd co-contaminated paddy field, and the effecting mechanisms are elucidated by a series of advanced techniques. The results showed that the grains inorganic As and Cd was remarkably decreased by 45 % and 66 % by the foliar spay of ZIF-8@Ge-132, respectively. ZIF-8@Ge-132 also reduced the As and Cd contents in rice tissues, except for Cd in leaves, where Cd content increased by 148 %. The image-based measurement of plant phenotypic traits and the elements of image analysis using Laser Ablation-ICP-MS (LA-ICP-MS) and Laser Scanning Confocal Microscopy (LSCM) revealed that the possible mechanisms for the reduction of As and Cd in rice grains were as follows: (i) the thickening of the xylem in roots significantly retarded As and Cd absorption by rice plants. (ii) co-accumulation of Ge and Cd in the leaf vascular system likely contributed to the high Cd retention in rice leaves. (iii) antagonistic effects of Zn suppressed the uptake and transport of As in roots/leaves, resulting a lower As accumulation in rice grains.

6.
Huan Jing Ke Xue ; 43(11): 5234-5243, 2022 Nov 08.
Artigo em Chinês | MEDLINE | ID: mdl-36437095

RESUMO

The interaction between different elements is an efficient means to control the heavy metal accumulation in crops. Phosphorus (P) and zinc (Zn), as essential nutrient elements of plants, have been shown to have important impacts on cadmium (Cd) accumulation in crops through interactions with each other. However, the function of the simultaneous interaction of P, Zn, and Cd on vegetable growth and Cd accumulation remains unclear. Herein, using a single-factor level design with two alternating fixed factors, pot experiments were conducted to study the impact and mechanism of this simultaneous interaction at different levels of P, Zn, and Cd on Brassica campestris L. growth, antioxidant enzyme activity, and Zn and Cd accumulation with neutral purple soil as the substrate. The results showed that the addition of an appropriate amount of P and Zn could promote the growth of Brassica campestris L. and inhibit its Cd accumulation, through different mechanisms. P mainly reduced the Cd availability in soil and improved the crop resistance, whereas Zn mainly promoted the dilution effect by the crop growth and its physiological antagonism. The antioxidant capacity of Brassica campestris L. was significantly inhibited when 1 mg·kg-1 exogenous Cd was added to the soil, along with decreased activities of CAT and POD and high accumulation of MDA. Notably, both P and Zn could improve the antioxidant capacity and relieve Cd toxicity by increasing CAT activity, without obviously influencing POD activity. The highest yield of Brassica campestris L. (55.72 g·pot-1) was attained when the ratio of stress concentration for exogenous P, Zn, and Cd[ω(Cd):ω(Zn):ω(P)] was 1:10:200. Furthermore, the Cd content in the edible part was also lower than the national standard requirement of 50 µg·kg-1for Cd in green leafy vegetables (GB 2762-2017). In addition, the accumulation of Cd was further decreased when the proportion of P and Zn was increased, along with a decreased yield of the vegetable. Therefore, a proper application of P and Zn fertilizers could simultaneously reduce Cd accumulation and increase crop yield and thus contribute to achieving safe vegetable production.

7.
Metabolites ; 12(11)2022 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-36355151

RESUMO

Cardiac function is closely related to heart metabolism. Heart failure patients undergoing LVAD support have shown varying degrees of remodeling of both cardiac function and morphology. However, the metabolic changes in patients with different outcomes are unclear. This study aimed to identify metabolic differences and evaluate metabolomics-based biomarkers in patients with non-improved/improved cardiac function after LVAD support. Sixteen patients were enrolled in this study. Plasma samples were analyzed by using untargeted metabolomic approaches. Multivariate statistical analysis and a Mann-Whitney U-test was performed to clarify the separation in metabolites and to identify changes in plasma metabolites between the two groups, respectively. The efficacy of candidate biomarkers was tested by the area under the curve receiver operating characteristic curve. Using the Metabolomics Standards Initiative level 2, a total of 1542 and 619 metabolites were detected in the positive and negative ion modes, respectively. Enrichment analysis showed that metabolites in improved cardiac function patients were mainly involved in carbohydrate metabolism and amino acid metabolism. Metabolites from non-improved cardiac function patients were mainly involved in hormone metabolism. Furthermore, we found tris(hydroxymethyl)aminomethane and 5-guanidino-3-methyl-2-oxopentanoic acid could serve as biomarkers to predict whether a patient's cardiac function would improve after LVAD support.

8.
Artigo em Inglês | MEDLINE | ID: mdl-36436019

RESUMO

Myeloid-derived suppressor cells (MDSCs) are one of the tumor-infiltrating immune cell population, which play a powerful role in inhibiting anti-tumor immune response. Our previous studies have shown that STAT3 blockade can decrease the number of MDSCs in tumor microenvironment. However, it is unclear for the molecular mechanism of down-regulation MDSCs with STAT3 inhibitor. In this study, we first detected and analyzed the expression of p-STAT3, CD33, CD14, CD39 and CD73 via oral squamous cell carcinoma (OSCC) tissue array. We found that p-STAT3 was positively correlated with CD14, CD33, CD39, and CD73 in OSCC patient specimens. Then we found STAT3 blockade with S3I-201 reduced the expression of CD39/CD73 and the synthesis of adenosine, as well as inhibiting monocytes to MDSCs differentiation in vitro. Furthermore, we found that S3I-201 displayed prominent anti-tumor efficacy in C3H/He OSCC mouse model via inhibiting CD39/CD73-adenosine signal pathway and decreasing MDSCs. These results suggest that STAT3 signal can induce the differentiation of monocytes into MDSCs in tumor microenvironment depending on CD39/CD73-adenosine signal pathway and STAT3 blockade is a promising therapeutic strategy for OSCC.

9.
Appl Spectrosc ; : 37028221141709, 2022 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-36368897

RESUMO

Cadmium (Cd) is a highly toxic heavy metal that is widely distributed in soils at low concentrations. Its volatilization loss occurs at an ashing temperature higher than 350 °C. Accordingly, the accurate determination of its concentration in soils often requires the addition of chemical modifiers by graphite furnace atomic absorption spectrometry (GFAAS) to improve the thermal stability of Cd. In this work, a metal-organic framework (MOF, UIO-66-NH2) was utilized as a matrix modifier to improve accuracy in determining Cd in soils using GFAAS. The Cd signal, as influenced by drying and pyrolysis temperature and special gas, was also investigated, and the measuring conditions were optimized (2 µg L-1 Cd concentrations, matrix modifier: UIO-66-NH2, pyrolysis temperature: 550 °C, atomization temperature: 1650 °C, and special gas: air). The limit of detection (LOD) and the characteristic mass for Cd in soil were 3.84 ng g-1 and 0.80 pg, respectively. Five soil standard reference materials (SRMs) were analyzed for Cd to verify the effectiveness of the optimized measuring conditions. The soil Cd concentrations obtained were in remarkable agreement with the reference values. Moreover, the mechanism of UIO-66-NH2 on the graphite platform was investigated in the drying and pyrolysis steps by scanning electron microscopy (SEM), Fourier transform infrared (FT-IR), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). During the drying steps, many functional groups of the matrix were activated, and Cd2+ could be captured on its surface. After the pyrolysis step, the structure matrix collapsed and was partially decomposed into ZrO2, and subsequently, an intermediate state of ZrO2-(CdO) appeared at high temperatures, which came from the active binding sites on the zirconium oxide octahedron nodes.

10.
Dig Dis ; 2022 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-36257291

RESUMO

BACKGROUND: Immune checkpoint inhibitors (ICIs) have improved survival outcomes and resulted in long-term responses in primary liver cancer in some patients. However, its efficacy is limited by the risk of tumor recurrence, resulting in rapid death and graft loss if patients are not selected appropriately. Therefore, it is necessary to identify patients suitable for such therapy. METHODS: 215 patients with primary liver cancer with immunotherapy were screened between August 2018 and October 2020 as a training set and our validation set were included 71 patients of hepatocellular carcinoma from Jiangxi Cancer Hospital, The Second Affiliated Hospital of Nanchang Medical College from May 2019 to July 2021. The primary endpoint was the disease control rate (DCR), and the secondary endpoints were overall survival (OS) and progression-free survival (PFS). RESULTS: In the traing set, Neutrophil-leukocyte ratio (NLR) >3, Alpha-fetoprotein (AFP) level >20 ng/ml, distant metastasis at baseline, and absence of combination with targeted therapy were associated with non-DCR in the training set. Moreover, NLR >3 and AFP level >20 ng/ml were also independently associated with OS. Furthermore, a hepatic immune predictive index (HIPI) based on NLR >3 and AFP level >20 ng/mL was developed and associated with worse clinical outcomes. In validation set, HIPI was associated with overall survival. CONCLUSION: Baseline hepatic immune predictive index based on NLR and AFP level is an effective indicator in ICI-treated patients with primary liver cancer. Our findings may help guide the selection and on-treatment strategies for immunotherapies for primary liver cancer patients.

11.
Opt Express ; 30(11): 19212-19221, 2022 May 23.
Artigo em Inglês | MEDLINE | ID: mdl-36221705

RESUMO

Combining digital information science with metasurface technology is critical for achieving arbitrary electromagnetic wave manipulation. However, there is a scarcity of contemporary scholarly studies on this subject. In this paper, we propose an Ultraviolet (UV) sensing metasurface for programmable electromagnetic scattering field manipulation by combining light control with a microwave field. The active sensing of UV light and the real-time reaction of the scattering are achieved by integrating four UV sensors on the metasurface. On the metasurface, a UV sensor ML8511 and a voltage driver module are coupled to control each row of the Positive-Intrinsic-Negative (PIN) diodes. Due to the light sensing capability of the UV sensor, the on or off state of the PIN diode integrated into the programmable metasurface can be switched efficiently through the change of light. When the incident wave changes, various discrete data are transmitted to the FPGA. Then the FPGA performs the corresponding voltage distribution to control the state of the PIN diode. Finally, different metasurface coding sequences are generated to realize different electromagnetic functions. As a result, the spatial distribution of sensing light by sensors can be used to determine the electromagnetic field and connect sensing optical information with the microwave field. The simulation and measured results show that this design is feasible. This work provides a dimension for electromagnetic waves modulation.

12.
World J Gastrointest Surg ; 14(9): 950-962, 2022 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-36185561

RESUMO

BACKGROUND: Totally laparoscopic gastrectomy (TLG) entails both gastrectomy and gastrointestinal reconstruction under laparoscopy. Compared with laparoscopic assisted gastrectomy (LAG), TLG has been demonstrated in many studies to require a smaller surgical incision, result in a faster postoperative recovery and less pain and have comparable long-term efficacy, which has been a research hotspot in recent years. Whether TLG is equally safe and feasible for elderly patients remains unclear. AIM: To compare the short-term efficacy of and quality of life (QOL) associated with TLG and LAG in elderly gastric cancer (GC) patients. METHODS: The clinicopathological data of 462 elderly patients aged ≥ 70 years who underwent LAG or TLG (including distal gastrectomy and total gastrectomy) between January 2017 and January 2022 at the Department of General Surgery, First Medical Center, Chinese PLA General Hospital were retrospectively collected. A total of 232 patients were in the LAG group, and 230 patients were in the TLG group. Basic patient information, clinicopathological characteristics, operation information and QOL data were collected to compare efficacy. RESULTS: Compared with those in the LAG group, intraoperative blood loss in the TLG group was significantly lower (P < 0.001), and the time to first flatus and postoperative hospitalization time were significantly shorter (both P < 0.001). The overall incidence of postoperative complications in the TLG group was significantly lower than that in the LAG group (P = 0.01). Binary logistic regression results indicated that LAG and an operation time > 220 min were independent risk factors for postoperative complications in elderly patients with GC (P < 0.05). In terms of QOL, no statistically significant differences in various preoperative indicators were found between the LAG group and the LTG group (P > 0.05). Compared with the laparoscopic-assisted total gastrectomy group, patients who received totally laparoscopic total gastrectomy had lower nausea and vomiting scores and higher satisfaction with their body image (P < 0.05). Patients who underwent laparoscopic-assisted distal gastrectomy were more satisfied with their body image than patients in the totally laparoscopic distal gastrectomy group (P < 0.05). CONCLUSION: TLG is safe and feasible for elderly patients with GC and has outstanding advantages such as reducing intracorporeal blood loss, promoting postoperative recovery and improving QOL.

13.
Opt Express ; 30(19): 33574-33587, 2022 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-36242389

RESUMO

Metasurface-based structural-colors are usually implemented by changing the dimensions of nanostructures to produce different spectral responses. Therefore, a single-size nanostructured metasurface usually cannot display structural-colors since it has only one design degree of freedom (DOF), i.e., the orientation angles of nanostructures. Here, we show structural-color nanoprinting images can be generated with a single-size nanostructured metasurface, enabled by designing the anisotropic nanostructure with different spectral responses along its long- and short-axis directions, respectively. More interestingly, the concept of orientation degeneracy of nanostructures can be applied in the metasurface design, which shows two spectral modulations can be implemented under different polarization directions of output light, thus extending the color-nanoprinting from single-channel to dual-channel. The proposed dual-channel metasurface used for anticounterfeiting color-nanoprinting has presented the advantages of ultra-compactness, high information capacity, and vivid colors, which can develop broad applications in fields such as high-end anticounterfeiting, high-density information storage, optical encryption, etc.

14.
Micromachines (Basel) ; 13(10)2022 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-36296142

RESUMO

With the rapid development of 5G, artificial intelligence (AI), and high-performance computing (HPC), there is a huge increase in the data exchanged between the processor and memory. However, the "storage wall" caused by the von Neumann architecture severely limits the computational performance of the system. To efficiently process such large amounts of data and break up the "storage wall", it is necessary to develop processing-in-memory (PIM) technology. Chiplet combines processor cores and memory chips with advanced packaging technologies, such as 2.5D, 3 dimensions (3D), and fan-out packaging. This improves the quality and bandwidth of signal transmission and alleviates the "storage wall" problem. This paper reviews the Chiplet packaging technology that has achieved the function of PIM in recent years and analyzes some of its application results. First, the research status and development direction of PIM are presented and summarized. Second, the Chiplet packaging technologies that can realize the function of PIM are introduced, which are divided into 2.5D, 3D packaging, and fan-out packaging according to their physical form. Further, the form and characteristics of their implementation of PIM are summarized. Finally, this paper is concluded, and the future development of Chiplet in the field of PIM is discussed.

15.
Diagn Interv Radiol ; 2022 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-36287132

RESUMO

PURPOSE The high rate of recurrence of hepatocellular carcinoma (HCC) after radical hepatectomy is an important factor that affects the long-term survival of patients. This study aimed to develop a computed tomography (CT) images-based 3-dimensional (3D) convolutional neural network (CNN) for the preoperative prediction of early recurrence (ER) (≤2 years) after radical hepatectomy in patients with solitary HCC and to compare the effects of segmentation sampling (SS) and non-segmentation sampling (NSS) on the prediction performance of 3D-CNN. METHODS Contrast-enhanced CT images of 220 HCC patients were used in this study (training group=178 and test group=42). We used SS and NSS to select the volume-of-interest to train SS-3D-CNN and NSS-3D-CNN separately. The prediction accuracy was evaluated using the test group. Finally, gradient-weighted class activation mappings (Grad-CAMs) were plotted to analyze the difference of prediction logic between the SS-3D-CNN and NSS-3D-CNN. RESULTS The areas under the receiver operating characteristic curves (AUCs) of the SS-3D-CNN and NSS3D-CNN in the training group were 0.824 (95% CI: 0.764-0.885) and 0.868 (95% CI: 0.815-0.921). The AUC of the SS-3D-CNN and NSS-3D-CNN in the test group were 0.789 (95% CI: 0.637-0.941) and 0.560 (95% CI: 0.378-0.742). The SS-3D-CNN could stratify patients into low- and high-risk groups, with significant differences in recurrence-free survival (RFS) (P < .001). But NSS-3D-CNN could not effectively stratify them in the test group. According to the Grad-CAMs, compared with SS-3D-CNN, NSS-3D-CNN was obviously interfered by the nearby tissues. CONCLUSION SS-3D-CNN may be of clinical use for identifying high-risk patients and formulating individualized treatment and follow-up strategies. SS is better than NSS in improving the performance of 3D-CNN in our study.

16.
Opt Express ; 30(16): 28882-28891, 2022 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-36299075

RESUMO

Because of unique superiorities of planar optical devices based on metalens on manipulating amplitude, phase, polarization, wavelength of incident light, metalenses have great prospects to replace traditional catadioptric optical components, especially in imaging and optoelectronic integration. However, the research of metalens has focused on visible or near-infrared wavelength in the past few years and little attention was paid to the long-wavelength infrared metalens. Here, we demonstrate the largest aperture, high numerical aperture, and polarization-independent metalens operating at long-wavelength infrared. The metalens has a numerical aperture of 0.45 at the center wavelength of10 µm. The aperture of the metalens is 80 mmwhich is much larger than the existing level we know. It has high-resolution imaging ability with focusing incident light down to a spot as small as ∼1.04λ. Ambient light imaging experiments are carried out to show the performance of the metalens. In addition, metalens is flimsy, large-scale and low-cost, which provides an effective solution for the development of ultra-lightweight and compact optical devices for LWIR technology.

17.
Anal Chem ; 94(45): 15729-15737, 2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-36315965

RESUMO

Currently, single-cell lipidomic mass spectrometry (MS) techniques are mostly limited to detection of high-abundance phosphatidylcholines (PCs). Herein, for enhancing the coverage to low-abundance sphingolipids in single-cell analysis, in-tube solid-phase microextraction (SPME) was combined with a single-probe MS system for selective enrichment of sphingolipids during singe-cell sampling. From the results, a lab-made single probe with a 30 µm tip size proved to be able to resolve the axon from the cell body of neuron HT22 in ambient conditions. TiO2 was immobilized onto the inner wall of the transfer capillary of the single-probe device for online selective capture of sphingolipids in ammonia-acetonitrile and rapid desorption in formic acid-methanol. The results showed that the breakthrough volume of the capillary with sample loading flow rate at 500 nL/min was >14 µL. Standard experiments showed that the signals of cerebroside (CB), ceramide (Cer), and sphingomyelin (SM) were largely enhanced after selective capture in the coated capillary, while PCs were totally removed. The reusability (>10 times) and stability of the lab-made TiO2-coated capillary was verified. By introducing the coated capillary into the single-probe MS system, the new system proved to have low detection limits of SM, Cer, and CB (0.007-0.027 µg/mm2) and acceptable linearity (r > 0.98) and repeatability (RSD < 30%). Lipid coverage of the new method to SMs and CBs proved to be largely improved (SM, 21 vs 2; CB, 10 vs 0) with the new method in comparison to conventional single-probe MS without selective capture by ambient analysis of a single spot of rat cerebellum. Finally, the new system was used to perform single-neuron analysis of sphingolipids in the control and lipopolysaccharide (LPS)-treated HT22 with differentiation of the cell body from the axonal synapse. Results showed that 5 sphingolipids had significantly higher concentrations in the synapse than in the cell body, while 3 oxidized sphingolipids had significantly higher levels in the cell body than in the synapse. After LPS treatment, most of the sphingolipids largely decreased and became more accumulated in the synapse, providing new information on LPS-induced neuroinflammation.


Assuntos
Esfingolipídeos , Espectrometria de Massas em Tandem , Ratos , Animais , Espectrometria de Massas em Tandem/métodos , Corpo Celular , Lipopolissacarídeos , Ceramidas , Esfingomielinas , Sinapses , Neurônios
18.
Arterioscler Thromb Vasc Biol ; 42(12): 1429-1446, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36200446

RESUMO

BACKGROUND: Exploring the mechanisms of valvular heart disease at the cellular level may be useful to identify new therapeutic targets; however, the comprehensive cellular landscape of nondiseased human cardiac valve leaflets remains unclear. METHODS: The cellular landscapes of nondiseased human cardiac valve leaflets (5 aortic valves, 5 pulmonary valves, 5 tricuspid valves, and 3 mitral valves) from end-stage heart failure patients undergoing heart transplantation were explored using single-cell RNA sequencing. Bioinformatics was used to identify the cell types, describe the cell functions, and investigate cellular developmental trajectories and interactions. Differences among the 4 types of cardiac valves at the cellular level were summarized. Pathological staining was performed to validate the key findings of single-cell RNA sequencing. An integrative analysis of our single-cell data and published genome-wide association study-based and bulk RNA sequencing-based data provided insights into the cell-specific contributions to calcific aortic valve diseases. RESULTS: Six cell types were identified among 128 412 cells from nondiseased human cardiac valve leaflets. Valvular interstitial cells were the largest population, followed by myeloid cells, lymphocytes, valvular endothelial cells, mast cells, and myofibroblasts. The 4 types of cardiac valve had distinct cellular compositions. The intercellular communication analysis revealed that valvular interstitial cells were at the center of the communication network. The integrative analysis of our single-cell RNA sequencing data revealed key cellular subpopulations involved in the pathogenesis of calcific aortic valve diseases. CONCLUSIONS: The cellular landscape differed among the 4 types of nondiseased cardiac valve, which might explain their differences in susceptibility to pathological remodeling and valvular heart disease.


Assuntos
Estenose da Valva Aórtica , Calcinose , Insuficiência Cardíaca , Doenças das Valvas Cardíacas , Humanos , Estenose da Valva Aórtica/patologia , Valva Aórtica/patologia , Calcinose/metabolismo , Células Endoteliais/metabolismo , Estudo de Associação Genômica Ampla , Células Cultivadas , Doenças das Valvas Cardíacas/genética , Doenças das Valvas Cardíacas/cirurgia , Doenças das Valvas Cardíacas/complicações , Insuficiência Cardíaca/metabolismo
19.
World J Emerg Med ; 13(5): 355-360, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36119776

RESUMO

BACKGROUND: This study aimed to establish an effective nomogram to predict the survival of heat stroke (HS) based on risk factors. METHODS: This was a retrospective, observational multicenter cohort study. We analyzed patients diagnosed with HS, who were treated between May 1 and September 30, 2018 at 15 tertiary hospitals from 11 cities in Northern China. RESULTS: Among the 175 patients, 32 patients (18.29%) died before hospital discharge. After the univariate analysis, mechanical ventilation, initial mean arterial pressure <70 mmHg, maximum heart rate, lab results on day 1 (white blood cell count, alanine aminotransferase, creatinine), and Glasgow admission prediction score were included in multivariate analysis. Multivariate Cox regression showed that invasive ventilation, initial mean arterial pressure <70 mmHg (1 mmHg=0.133 kPa), and Glasgow admission prediction score were independent risk factors for HS. The nomogram was established for predicting 7-d and 14-d survival in the training cohort. The nomogram exhibited a concordance index (C-index) of 0.880 (95% confidence interval [95% CI] 0.831-0.930) by bootstrapping validation (B=1,000). Furthermore, the nomogram performed better when predicting 14-d survival, compared to 7-d survival. The prognostic index cut-off value was set at 2.085, according to the operating characteristic curve for overall survival prediction. The model showed good calibration ability in the internal and external validation datasets. CONCLUSION: A novel nomogram, integrated with prognostic factors, was proposed; it was highly predictive of the survival in HS patients.

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