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1.
Bioorg Med Chem ; 28(7): 115375, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32122753

RESUMO

Alkyne is a useful functionality incorporated in proteins for site-selective bioconjugation reactions. Although effective bioconjugation reactions such as copper(I)-catalyzed and/or copper-free 1,3-dipolar cycloadditions of alkynes and azides are the most common approaches, the development of new alkyne-based bioconjugation reactions is still an ongoing interest in chemical biology. In this work, a new approach has been developed for selective modification of alkyne-linked peptides and proteins through the formation of arylacetylenes by a cross-coupling reaction of 6-membered ring cyclometalated gold(III) (C^N) complexes (HC^N = 2-arylpyridines) with terminal alkynes. Screening of the reaction conditions with a series of cyclometalated gold(III) complexes with phenylacetylene gave an excellent yield (up to 82%) by conducting the reaction in slightly alkaline aqueous conditions. The reaction scope was expanded to various alkynes, including alkyne-linked peptides to achieve up to >99% conversion. Using fluorescent dansyl (1l) and BODIPY (1m)-linked gold(III) complexes, alkyne-linked lysozyme has been selectively modified.

2.
Cell Mol Neurobiol ; 2020 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-32130572

RESUMO

Sevoflurane is a widely used obstetric general anesthetic, but the neurotoxic effects of late-pregnancy exposure to one minimum alveolar concentration ([MAC], 2.5%) of sevoflurane on offspring remain unclear. We investigated whether exposure to 2.5% sevoflurane during late pregnancy would affect offspring hippocampal neuronal development and neurocognitive function. On gestational day 18 (G18), rats were randomly treated with 2.5% sevoflurane in 50% oxygen for 1 (Sev × 1), 3 (Sev × 3), or 6 h (Sev × 6). The neuronal apoptosis rate and mature brain-derived neurotrophic factor (mBDNF) and postsynaptic density protein 95 (PSD-95) expression levels were measured in offspring hippocampi on postnatal day 1 (P1) and P35. Dendritic spine formation and cognitive function were examined on P35. The neuronal apoptosis rate was enhanced, and mBDNF and PSD-95 levels were reduced in the Sev × 3 and Sev × 6 groups on P1. mBDNF and PSD-95 levels were also decreased in the Sev × 6 group on P35. The error rate was elevated in the maze test, whereas dendritic spine density and long-term potentiation (LTP) were reduced in the Sev × 6 group on P35. To determine whether exposure to an enriched environment (EE) would ameliorate sevoflurane's neurotoxic effects, offspring from another Sev × 6 group were exposed to either a standard environment (SE) or an EE. Lower error rates and greater dendritic spine densities and LTP were found in the Sev × 6 + EE vs. Sev × 6 + SE group. Collectively, we showed that exposing rats to 1 MAC sevoflurane for 3 h during late pregnancy increased neuronal apoptosis in neonates but did not impair neuronal development or cognitive function in juvenile rats, whereas a 6-h exposure impaired neuronal development and cognitive function in juvenile rats, effects that were attenuated by an EE.

3.
J Hazard Mater ; 392: 122331, 2020 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-32097858

RESUMO

The emergence of more and more persistent organic molecules as contaminants in water simulates research towards the development of more advanced technologies, among which photocatalysis is a feasible choice. However, it is still challenging to design a photocatalyst that fulfills all the requirements for industrial application, i.e., active under visible-light irradiation, shape with handy convenience, highly uniform distribution of active sites, substrate with excellent electronic properties, etc. In this study, we report an attempt to solve these issues at once by designing a film-like photocatalyst with uniform distribution of nitrogen-doped ZnO nanoparticles along nitrogen-doped carbon ultrafine nanofibers with three-dimensional interconnected structure. Under visible-light irradiation, the product exhibited remarkable reactivity for the degradation of two model pollutants tetracycline hydrochloride and 2,4-dichlorophenol within 100 min. The cyclic experiments demonstrated only a slight loss (ca. 5 %) of reactivity after five consecutive photocatalytic reactions. We also investigated the detailed relationship between the structural features and the superior properties of this product, as well as the degradation mechanisms. The convenient shape of the product with excellent performances for the treatment of real polluted water increases its suitability for larger scale application. Our work provides a rational design of photocatalysts for environmental remediation.

4.
Mol Cell Probes ; : 101544, 2020 Feb 25.
Artigo em Inglês | MEDLINE | ID: mdl-32109535

RESUMO

The duplex real-time PCR assay based on SYBR Green І was developed for detection of porcine epidemic diarrhea virus (PEDV) and porcine bocavirus (PBoV) 3/4/5 genotypes simultaneously. Two pairs of specific primers were designed targeting the N gene sequence of PEDV and VP1 gene sequence of PBoV3/4/5. PEDV and PBoV3/4/5 could be distinguished by their different melting temperatures (Tm) in one sample. The Tm value of PEDV was 83.5 °C, and the Tm value of PBoV3/4/5 was 78.5 °C, while other swine pathogens showed no specific melting peaks. The detection limits of this assay were 10 copies/µL for both PEDV and PBoV3/4/5. A total of sixty-three intestinal tissue samples were collected from piglets suffering from diarrhea, and the viral nucleic acids detected and identified by the real-time PCR assay and conventional PCR assay. The duplex real-time PCR detection results showed that the prevalence of PEDV and PBoV3/4/5 was 85.7% and 46%, respectively, and the co-infection rate of the two viruses was 28.6%. These results indicated that this duplex real-time PCR assay was a sensitive, specific and reproducible method for differentiating PEDV and PBoV3/4/5 or their co-infection.

5.
Artigo em Inglês | MEDLINE | ID: mdl-32108523

RESUMO

Patients with peripheral arterial disease (PAD) have an accentuated exercise pressor reflex (EPR) during exercise of the affected limb. The underlying hemodynamic changes responsible for this, and its effect on blood flow to the exercising extremity is unclear. We tested the hypothesis that the exaggerated EPR in PAD is mediated by an increase in total peripheral resistance (TPR), which augments redistribution of blood flow to the exercising limb. Eleven PAD patients and 11 age, and sex-matched subjects without PAD performed dynamic plantar flexion (PF) using the most symptomatic leg at progressive workloads of 2 to 12 kg (increased by 1 kg/min until onset of fatigue). We measured heart rate, beat-by-beat blood pressure (BP), femoral blood flow velocity (FBV), and muscle oxygen saturation (SmO2) continuously during the exercise. Femoral blood flow (FBF) was calculated from FBV and baseline femoral artery diameter. Stroke volume (SV), cardiac output (CO), and TPR were derived from the BP tracings. Mean arterial blood pressure and TPR were significantly augmented in PAD compared to control during PF. FBF increased during exercise to an equal extent in both groups. However, SmO2 of the exercising limb remained significantly lower in PAD compared to control. We conclude that the exaggerated pressor response in PAD is mediated by an abnormal TPR response, which augments redistribution of blood flow to the exercising extremity, leading to an equal rise in FBF compared to controls. However, this increase in FBF is not sufficient to normalize the SmO2 response during exercise in patients with PAD.

6.
Ecotoxicol Environ Saf ; 193: 110344, 2020 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-32092583

RESUMO

To identify seasonal fluxes and sources of dissolved inorganic nitrogen (DIN) wet deposition, concentrations and δ15N signatures of nitrate (NO3-) and ammonium (NH4+) in wet precipitation were measured at four typical land-use types in the Three Gorges reservoir (TGR) area of southwest China for a one-year period. Higher DIN fluxes were recorded in spring and summer and their total fluxes (averaged 7.58 kg N ha-1) were similar to the critical loads in aquatic ecosystems. Significant differences of precipitation δ15N were observed for NH4+-N between town and wetland sites in spring and between urban and rural sites in summer. For NO3--N, significant differences of precipitation δ15N were observed between town and rural sites in spring and between urban and town sites in autumn, respectively. Quantitative results of NO3--N sources showed that both biomass burning and coal combustion had higher fluxes at the urban site especially in winter (0.18 ± 0.09 and 0.19 ± 0.08 kg N ha-1), which were about three times higher than those at the town site. A similar finding was observed for soil emission and vehicle exhausts in winter. On the whole, DIN wet deposition averaged at 12.13 kg N ha-1 yr-1 with the urban site as the hotspot (17.50 kg N ha-1 yr-1) and regional NO3--N fluxes had a seasonal pattern with minimum values in winter. The contribution to NO3--N wet deposition from biomass burning was 26.1 ± 14.1%, which is the second dominant factor lower than coal combustion (26.5 ± 12.6%) in the TGR area during spring and summer. Hence N emission reduction from biomass burning, coal combustion and vehicle exhausts should be strengthened especially in spring and summer to effectively manage DIN pollution for the sustainable development in TGR area.

7.
Dalton Trans ; 2020 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-32067026

RESUMO

A family of new dinuclear lanthanide complexes as the simplest entities showing intramolecular magnetic interactions, [Ln2(dbm)2(L)2(CH3OH)2] (Ln = Tb (1), Dy (2), Ho (3), Er (4), Yb (5), Lu (6)), [Ln2(acac)2(L)2(EtOH)2] (Ln = Dy (7), Er (8)), [Dy2(TTA)2(L)2(CH3OH)2]·2CH2Cl2 (9) and [Dy2(tfa)2(L)2(CH3OH)2] (10) (H2L = N'-(2-hydroxy-5-methylphenyl)-pyrazine-2-carbohydrazide, Hdbm = 1,3-diphenyl-1,3-propanedione, Hacac = acetylacetone, HTTA = 2-thenoyltrifluoroacetone, Htfa = trifluoroacetylacetone), were constructed successfully by the reaction of a Schiff base ligand H2L and four different ß-diketonate salts. As for complexes 4, 5 and 8, all exhibit the characteristic emission peaks of the corresponding Er3+, Yb3+ and Er3+ ions, respectively. Meanwhile, the excitation wavelength (510 nm) of 5 is located in the visible region, confirming its significant potential application value. Magnetic studies indicate that complexes 9 and 10 exhibit characteristic slow relaxation of magnetization with the energy barriers (Ueff) of 102 K for 9 and 140 K for 10 under a zero dc field. Under the optimized dc fields, slow magnetic relaxations are present in 2 and 7, and the Ueff values of 9 and 10 have been improved. This proves that the ß-diketonate co-ligands deserve an important role in regulating Dy-SMMs influenced by the diverse perturbations of the axial crystal field originating from minor changes in the coordination environment.

8.
Mol Cell Probes ; 50: 101524, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31972226

RESUMO

In the present study, the SYBR green I-based duplex quantitative polymerase chain reaction (qPCR) was developed for simultaneous detection of classical swine fever virus (CSFV) and porcine circovirus 3 (PCV3). The assay was used to detect both CSFV and PCV3 in one sample by their distinct melting temperatures (melting peaks at 87°C for CSFV and 81.5 °C for PCV3), and no specific fluorescence signals were detected for other non-targeted porcine pathogens. The assay had a high degree of linearity (R2 > 0.998) with the detection limits of 23 copies/µL for CSFV and 36 copies/µL for PCV3, and exhibited high repeatability and reproducibility with a low coefficient of variation below 2.0% in both intra- and inter-assay. In this study, 130 clinical samples collected from sick pigs in the field were tested by this assay with the positive rates of 9.23% (12/130) for CSFV and 21.54% (28/130) for PCV3 respectively, and the positive rate of CSFV and PCV3 co-infection was 6.92% (9/130). Our results showed that the developed method was a reliable diagnostic tool to monitor and survey CSFV, PCV3 and CSFV/PCV3 co-infection in the field.

9.
Neurorehabil Neural Repair ; 34(2): 122-133, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31904298

RESUMO

Background. Neuroimaging studies of spinal cord injury (SCI) have mostly examined the functional organization of the cortex, with only limited focus on the subcortical substrates of the injury. However, thalamus is an important modulator and sensory relay that requires investigation at a subnuclei level to gain insight into the neuroplasticity following SCI. Objective. To use resting-state functional magnetic resonance imaging to examine the functional connectivity (FC) of thalamic subnuclei in complete SCI patients. Methods. A seed-based connectivity analysis was applied for 3 thalamic subnuclei: pulvinar, mediodorsal, and ventrolateral nucleus in each hemisphere. A nonparametric 2-sample t test with permutations was applied for each of the 6 thalamic seeds to compute FC differences between 22 healthy controls and 19 complete SCI patients with paraplegia. Results. Connectivity analysis showed a decrease in the FC of the bilateral mediodorsal nucleus with right superior temporal gyrus and anterior cingulate cortex in the SCI group. Similarly, the left ventrolateral nucleus exhibited decreased FC with left superior temporal gyrus in SCI group. In contrast, left pulvinar nucleus demonstrated an increase in FC with left inferior frontal gyrus and left inferior parietal lobule in SCI group. Our findings also indicate a negative relationship between postinjury durations and thalamic FC to regions of sensorimotor and visual cortices, where longer postinjury durations (~12 months) is associated with higher negative connectivity between these regions. Conclusion. This study provides evidence for reorganization in the thalamocortical connections known to be involved in multisensory integration and affective processing, with possible implications in the generation of sensory abnormalities after SCI.

10.
Bull Environ Contam Toxicol ; 104(3): 380-385, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31932904

RESUMO

In this study, three soil amendments (inorganic, liming, or organic-inorganic materials) were used in a Cd-contaminated purple field soil to investigate their impacts on soil Cd availability, enzyme (urease, catalase, sucrase, and acid phosphatase) activities, microbial biomass (carbon/nitrogen) and type (bacteria, fungi, and actinomycetes) in mustard and corn trials. Results showed that soil amendments generally decreased soil exchangeable Cd, fungi and bacterial populations while increasing the activities of all the four soil enzymes tested, microbial biomass carbon and populations of actinomycetes (p < 0.05). Soil pH and microbial biomass nitrogen did not exhibit any significant response (p > 0.05) whereas stronger effects appeared in soil organic matter and available nutrients (nitrogen, phosphorous and potassium; p < 0.05). However, only soil available phosphorous significantly correlated with soil microbial activity in both mustard and corn trails (p < 0.05). Thus, application of phosphorous-containing amendments should be considered for promoting soil health in the remediation of the Cd-contaminated purple soils.

11.
Zhongguo Zhen Jiu ; 40(1): 54-7, 2020 Jan 12.
Artigo em Chinês | MEDLINE | ID: mdl-31930900

RESUMO

The value of the "touching-bone" acupuncture technique in clinical application was explained through the investigation on the origin of the theory of the "touching-bone" acupuncture technique, the analysis on the characteristics of acupoint selection, the introduction of clinical characteristics and the discussion on the mechanism of acupuncture in treatment. The "touching-bone" acupuncture technique refers to deep needling method, originated from the short needling and shu needling of the ancient needling methodslisted in the Internal Classic. The target points are the reaction sites on meridian near to bone and the attachments of soft tissues on bone. During the needle insertion, the needle tip is thrust deeply to the bone or the needle body is closely attached to the bone so as to stimulate periosteum specifically. This needling technique contributes to the satisfactory effect on spasmodic, deep-located and intractable pain disorder, motor system diseases, mental diseases and cerebral diseases, etc. Hence, this acupuncture technique deserves to be promoted in clinical application and explored in research.


Assuntos
Terapia por Acupuntura , Meridianos , Agulhas
12.
Environ Int ; 136: 105487, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31999974

RESUMO

BACKGROUND: The major components of traffic pollution particulate matter, diesel exhaust particles (DEPs), are airborne ultrafine particles (UFPs). DEPs can enter the central nervous system (CNS), where they may cause neurotoxicity. METHODS: We established murine models with intranasal DEPs instillation in male C57BL/6 and Nlrp3 knock-out (Nlrp3-/-) mice to investigate the effects of DEPs exposure on murine neurobehaviors and related mechanisms. Morris water maze (MWM) tests were performed to evaluate the learning and memory behaviors of mice following DEPs instillation. Metabolomics were assessed using an gas chromatography system coupled to a mass spectrometer. Real-time PCR and immunohistochemistry assays were used to analyze the mRNA and protein expression levels of target genes. Murine microglia, BV2 cells were employed to assay the effects of DEPs exposure in vitro. RESULTS: Intranasal administration of DEPs in mice led to impairment in hippocampal-dependent learning and memory. Moreover, this phenotype was linked to increased number of Iba-1+ microglia and NLRP3 inflammasome, as well as suppression of mitochondrial gene expression in the hippocampus of mice exposed to DEPs. Nlrp3-/- mice were resistant to DEPs-induced learning and memory impairment, concomitant with protection against the suppression of mitochondrial gene expression. Murine microglia cells (BV2) were exposed to DEPs in vitro and taurine was identified as one of the significantly suppressed metabolites in DEPs-treated microglia by metabolomics analysis. Supplementation with taurine efficiently rescued learning, memory and mitochondrial gene expression levels in the hippocampus of DEPs-exposed mice. CONCLUSIONS: Mechanistically, our study revealed that microglia-mediated NLRP3 inflammasome activation plays a deleterious role in DEPs-induced neurotoxicity by inhibiting mitochondrial gene expression. These results shed novel light on the potential value of nutritional supplementation against DEPs-induced neurotoxicity in individuals exposed to severe airborne traffic-related air pollutions.

13.
J Hazard Mater ; 384: 121285, 2020 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-31577969

RESUMO

Despite the global importance of atmospheric heavy metal input into agricultural soils, research has primarily focused on the amount of the depositions with limited attention given to the risk of the newly deposited heavy metals. To understand the remobilization of the newly deposited copper (Cu) and lead (Pb) from the atmosphere and explore the metals' mobility and bioavailability to rice (Oryza sativa L.), a soil transplant experiment was conducted in three areas along a gradient of atmospheric depositions. Approximately 61% of the Cu and 76% of the Pb depositions tended to be present in potentially mobile fractions. The soil retention of newly deposited Cu and Pb presented as higher mobile fractions than these in the original soil. The newly deposited Cu and Pb in soils only accounted for 0.34-8.7% and 0.07-0.29% of the total soil Cu and Pb pools, but they contributed 30-84% and 6-41% in rice tissues, respectively. A major implication of these findings is that once the heavy metal is deposited, it may be reactivated in soils and transported to aerial parts or foliar uptake into plant tissues, emphasizing the important role of the newly deposited Cu and Pb in contributing to the edible parts of crops.

14.
Cytometry A ; 97(1): 70-77, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31411813

RESUMO

High incidence and mortality rates for non-small-cell lung cancer (NSCLC) lead to low survival rates. Epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKI) are commonly first prescribed for NSCLC patients with EGFR mutations. However, most patients with sensitizing EGFR mutations become resistant to EGFR-TKI after 9-13 months treatment. Yiqi Chutan Tang (YQCT) has been prescribed as a treatment to this issue for over 20 years. In this report, high-performance liquid chromatography (HPLC) analysis, flow cytometry, western blot analysis, and functional annotation analysis were applied to uncover the molecular mechanisms of YQCT. Our results show the application of YQCT reduces gefitinib-induced drug resistance, induces slight cell cycle arrest, enhances gefitinib-induced apoptosis, and activates the autophagy. These results indicate that at the molecular level YQCT can reduce drug resistance and improve anti-cancer effects when associated with gefitinib, which could be a result of enhancement of apoptosis and autophagy in the EGFR-TKI resistant cells of NSCLC. This research provides a new treatment strategy for patients with EGFR-TKI resistance in NSCLC. © 2019 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry.

15.
J Colloid Interface Sci ; 562: 21-28, 2020 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-31830628

RESUMO

Cleaner production of photocatalyst with efficient property and stable reusability is of great importance for the elimination of organic pollutants in wastewater. Herein, we present a bacterial cellulose (BC)-based nanocomposite membrane with enhanced adsorption and photocatalytic degradation for dyes under UV radiation, by using BC nanofibers as a three-dimensional soft template, coated with the polydoamine (PDA) as a functional layer and a protective agent for immobilization of titanium dioxide (TiO2) nanoparticles. Compared with commercial P25, the as-prepared BC/PDA/TiO2 composite presented higher adsorption capacity for methyl orange, Rhodamine B and methylene blue, and the photocatalytic properties within 30 min after irradiation were further improved. BC/PDA/TiO2 also showed good stability, proved by the 5.5% reduction in photocatalytic capability after five cyclic tests. We expect our design could provide a facile and green approach with excellent photo-degradation performance for organic pollutants, providing further applications for photocatalysis and wastewater treatment.

16.
Sci Total Environ ; 707: 135789, 2020 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-31865082

RESUMO

Hazardous elements (HEs) and its deposition from atmosphere have become a major healthy and environmental concern worldwide, however, the risks to food safety of deposited HE are still largely unknown, especially for wheat grains (Triticum aestivum L.). This research was to characterize atmospheric deposition of high HEs [such as lead (Pb), cadmium (Cd) and arsenic (As)] at deposited site (TS) and assess its impacts on the soil and winter wheat accumulation in a lead­zinc smelter contaminated area of Central China. Results indicated that deposited As, Cd and Pb at TS site were 31.15, 15.94 and 281.86 mg m-2 during the winter wheat growing season, contributing 86.9%, 81.7% and 80.4% to the annual total, respectively. Most of deposited HEs were retained in top 35-cm soil, resulting in soil Cd concentration above the national soil regulatory limit (0.6 mg kg-1; GB 15618-2018). The newly deposited HEs could contribute 57.7-88.2% and 16.2-29.1% to total HEs of wheat grains in clean soil based on a soil transplanted experiment and in contaminated soil based on a two-year in-situ experiment, respectively. Moreover, the newly deposited HEs significantly increased HE concentration of wheat grain by 22.9-57.5% in the contaminated soil when an immobilizing amendment were applied (p < 0.05), and grain Cd concentration exceeded the national grain limit (0.1 mg kg-1; GB 2762-2017). Thus, deposited HE could be accumulated in soil and wheat grain and decrease the remediation effectiveness of passivation amendments in contaminated soils, which would raise a public awareness of HE deposition and provide a scientific data in support of environmental pollution control and remediation.

17.
Carbohydr Polym ; 229: 115512, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31826502

RESUMO

In recent decades, increased industrial activities have led to the release of various pollutants, such as toxic heavy metals, inorganic anions, and organics. It is imperative but challenging to develop an eco-friendly treatment technology with easy operation, low cost, and high efficiency. Here, we describe a design of magnetic purifier, which has biomass-based structure by blending attapulgite/chitosan (ATP/CS) composite with bacterial cellulose nanofibrils (BCNs). Compared to similar materials reported previously, our product exhibited efficient adsorption capacities towards various metal ions including Pb2+, Cu2+, and Cr6+, and anionic organic dyes including Congo red. The adsorption process could be well fitted by Langmuir isotherm and pseudo-second order equation. Additionally, the adsorption capacity only decreased less than 8% after five adsorption-desorption cycles. We expect our design will inspire more efforts to build a multifunctional water purifier with simple operation, and hopefully effectively remove pollutants from wastewater in future practical applications.

18.
Sci Rep ; 9(1): 17976, 2019 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-31784680

RESUMO

Western wheatgrass (Pascopyrum smithii Rydb.) is an important cool-season forage and turfgrass. However, due to seed dormancy and poor seedling vigor, it is difficult to develop high seed yield production systems, and assessing these components in response to seed yield. Based on multifactor orthogonally designed field experimental plots under various field management regimes, the effects of numbers of fertile tillers m-2 (Y1), spikelets/fertile tiller (Y2), florets/spikelet (Y3), seed numbers/spikelet (Y4), and seed weight (Y5) on seed yield (Z) were determined over three successive years. Correlation analysis indicated that fertile tillers (Y1) was the most important seed yield component. And the biggest contribution of those five yield component is fertile tillers (Y1), followed by seed numbers/spikelet (Y4), spikelets/fertile tiller (Y2), florets/spikelet (Y3) and seed weight (Y5), respectively. By using ridge regression analysis, we have developed an accurate model of seed yield with its five components. Finally, the results of synergism and antagonism among these yield components on seed yield showed that fertile tillers and seed numbers/spikelet had an antagonistic effect on seed yield. Therefore, selection for high seed yield by direct selection for large values of fertile tillers and seed numbers/spikelet would be the most effective breeding strategy for western wheatgrass.

19.
Med Sci Monit ; 25: 10114-10121, 2019 Dec 29.
Artigo em Inglês | MEDLINE | ID: mdl-31884510

RESUMO

BACKGROUND Long non-coding RNAs (LncRNAs) could potentially function as diagnostic markers for gastric carcinoma. Nevertheless, the expression profile and biological feature of LncRNAs in early gastric cancer (EGC) remains to be explored. MATERIAL AND METHODS LncRNA expression microarray analysis was performed on 6 paired EGC tissues. One deregulated LncRNA, LOC389332, was validated using a quantitative reverse-transcription polymerase chain reaction (qRT-PCR) assay using independent tissue samples and cell lines. The Cell Counting Kit-8 (CCK-8) assay and wound healing assay were conducted to evaluate its influences on the proliferation and migration of gastric cancer cells. LncRNA expression microarray and gene ontology (GO) analysis were also performed on the LOC389332 knockdown cell line model to explore the molecular feature of LOC389332 in gastric carcinoma. RESULTS The LncRNA expression profiling showed that 72 LncRNAs were significantly differentially expressed in EGC tissues. The results in the validation phase revealed that LOC389332 was remarkably overexpressed in gastric carcinoma tissues, precancerous lesions, and gastric cancer cells. Functional study showed that knockdown of LOC389332 expression could inhibit cell proliferation and migration. LncRNA expression microarray on the LOC389332 knockdown cell line model revealed that 393 mRNAs were differentially expressed. The GO enrichment analysis indicated that the downregulated genes were mainly associated with cell membrane function, signal transmission process, and cell adhesion process. CONCLUSIONS The LncRNA expression profile between EGC and gastritis tissues was significantly different. LOC389332 was potential non-coding oncogenes in gastric cancer, and it may perform its function through altering cell membrane function, signal transmission, and cell adhesion.

20.
Huan Jing Ke Xue ; 40(8): 3764-3773, 2019 Aug 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854786

RESUMO

The concentrations of eight heavy metal elements (V, Cr, Co, Ni, Cu, Zn, Sr, and Pb) and the isotopic composition of Pb and Sr were determined in vertical soil profiles of the drylands of Xiamen City using ICP-MS and TIMS. The vertical distributions and leaching migration patterns of the heavy metals were also analyzed. The results showed that most of the heavy metals (except for Sr) were not enriched relative background values at most profile depths, which indicated that heavy metal content is less affected by exogenous sources. The migration coefficients of Sr were greater than 0 throughout the entire soil profile; Zn and Co were enriched in the surface soil; and Cr, Ni, and Cu were depleted throughout the profile. V and Pb were less influenced by external activities. Sr and Pb showed clear vertical migration characteristics, and the migration depth of Sr was greater than that of Pb. Factor analysis showed that the heavy metals mainly originated from natural sources, followed by agricultural activities, transportation, and other complex anthropogenic activities. Enrichment factor analysis showed that Sr was affected by exogenous sources to varying extents. The isotopic composition analysis of Pb and Sr showed that Pb was mainly derived from the parent material, whereas Sr was interpreted as deriving from both the parent material and anthropogenic activities, and tended to migrate to the subsurface soil horizons (30-70 cm).

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