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1.
Clin Genet ; 2020 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-32185793

RESUMO

Follicle-stimulating hormone (FSH) and luteinizing hormone (LH) play critical roles in female reproduction, while the underlying genetic basis is poorly understood. Genome-wide association studies (GWASs) of FSH and LH levels were conducted in 2590 Chinese females including 1882 polycystic ovary syndrome (PCOS) cases and 708 controls. GWAS for FSH level identified multiple variants at FSHR showing genome-wide significance with the top variant (rs2300441) located in the intron of FSHR. The A allele of rs2300441 led to a reduced level of FSH in the PCOS group (ß = -.43, P = 6.70 × 10-14 ) as well as in the control group (ß = -.35, P = 6.52 × 10-4 ). In the combined sample, this association was enhanced after adjusting for the PCOS status (before: ß = -.38, P = 1.77 × 10-13 ; after: ß = -.42, P = 3.33 × 10-16 ), suggesting the genetic effect is independent of the PCOS status. The rs2300441 explained sevenfold higher proportion of the FSH variance than the total variance explained by the two previously reported FSHR missense variants (rs2300441 R2 = 1.40% vs rs6166 R2 = 0.17%, rs6165 R2 = 0.03%). GWAS for LH did not identify any genome-wide significant associations. In conclusion, we identified genome-wide significant association between variants in FSHR and circulating FSH first, with the top associated variant rs2300441 might be a primary contributor at the population level.

2.
BMC Endocr Disord ; 20(1): 19, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-32000752

RESUMO

BACKGROUND: Polycystic ovary syndrome (PCOS) is the most common reason of anovulatory infertility. Environmental factor is one of the main causes of PCOS, but its contribution to ovulatory dysfunction in PCOS remains unknown. METHODS: A total of 2217 infertile women diagnosed as PCOS according to Rotterdam criteria were recruited, including 1979 women with oligo-anovulation (OA group) and 238 women with normal -anovulation (non OA group). Besides, 279 healthy control women of reproductive age were enrolled as controls. RESULTS: Frequencies of snoring (PCOS-OA group, PCOS-non-OA group, control group: 29.30% vs 18.10% vs 11.50%, P < 0.01), smoking (37.70% vs 28.10% vs 12.20%, P < 0.01), plastic tableware usage (38.30% vs 28.10% vs 25.40%, P < 0.01) and indoor decoration (32.10% vs 24.80% vs 16.80%, P < 0.01) were highest in PCOS-OA group. After adjusted for multivariable, difference remained significant between PCOS-OA group and the other two groups. PCOS-OA women preferred a meat favorable diet compared to PCOS-non-OA group (54.60% vs 41.30%, P < 0.01). There was no difference between three groups in exercise, frequency of insomnia, and alcohol consumption. CONCLUSIONS: Smoking, snoring, hyper-caloric diet, plastic tableware usage and indoor decoration were found to be associated with an increased risk for ovulatory dysfunction in women suffering from PCOS.

3.
J Eukaryot Microbiol ; 67(1): 107-114, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31486160

RESUMO

Enterocytozoon bieneusi is an important opportunistic pathogen widely distributed in humans and animals that causes diarrhea or fatal diarrhea in immunocompromised hosts. To examine the infection status and molecular characteristics of E. bieneusi in pigs, 725 fecal samples were collected from pigs in six areas of Fujian Province. The E. bieneusi genotypes were identified based on the internal transcribed spacer (ITS) regions of the ribosomal RNA (rRNA) gene by nested PCR, and its population genetics were analyzed by multilocus sequence typing (MLST). The results showed that the infection rate of E. bieneusi was 24.4% (177/725), and 11 known genotypes (EbpC, EbpA, CHN-RR2, KIN-1, CHG7, CHS5, CM11, CHG23, G, PigEBITS, and D) and 2 novel genotypes (FJF and FJS) were identified. All the genotypes were found to be clustered into zoonotic Group 1. Moreover, 52 positive samples were successfully amplified at minisatellite and microsatellite loci and formed 48 distinct multilocus genotypes (MLGs). Further population structure analyses showed strong genetic linkage disequilibrium (LD) and several recombination events (Rm), indicating that E. bieneusi has a clonal population structure. This study is the first to investigate the prevalence and molecular characteristics of E. bieneusi in Fujian Province and could provide baseline data to control E. bieneusi infection in pigs and humans and deepen our understanding of the zoonotic risk of E. bieneusi and its distribution in China.

4.
Biomed Res Int ; 2019: 2824017, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31781607

RESUMO

Parasitic Entamoeba spp. can infect many classes of vertebrates including humans and pigs. Entamoeba suis and zoonotic Entamoeba polecki have been identified in pigs, and swine are implicated as potential reservoirs for Entamoeba histolytica. However, the prevalence of Entamoeba spp. in pigs in southeastern China has not been reported. In this study, 668 fecal samples collected from 6 different regions in Fujian Province, southeastern China, were analyzed to identify three Entamoeba species by nested PCR and sequencing analysis. The overall prevalence of Entamoeba spp. was 55.4% (370/668; 95% CI 51.6% to 59.2%), and the infection rate of E. polecki ST1 was the highest (302/668; 45.2%, 95% CI 41.4% to 49.0%), followed by E. polecki ST3 (228/668; 34.1%, 95% CI 30.5% to 37.7%) and E. suis (87/668; 13.0%, 95% CI 10.5% to 15.6%). E. histolytica was not detected in any samples. Moreover, the coinfection rate of E. polecki ST1 and ST3 was 25.1% (168/668; 95% CI 21.9% to 28.4%), the coinfection rate of E. polecki ST1 and E. suis was 3.7% (25/668; 95% CI 2.3% to 5.2%), the coinfection rate of E. polecki ST3 and E. suis was 0.3% (2/668), and the coinfection rate of E. polecki ST1, E. polecki ST3, and E. suis was 4.0% (27/668; 95% CI 2.5% to 5.5%). A representative sequence (MK347346) was identical to the sequence of E. suis (DQ286372). Two subtype-specific sequences (MK357717 and MK347347) were almost identical to the sequences of E. polecki ST1 (FR686383) and ST3 (AJ566411), respectively. This is the first study to survey the occurrence and to conduct molecular identification of three Entamoeba species in southeastern China. This is the first report regarding mixed infections with E. suis, E. polecki ST1, and E. polecki ST3 in China. More research studies are needed to better understand the transmission and zoonotic potential of Entamoeba spp.

5.
Reprod Biomed Online ; 39(6): 947-954, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31734092

RESUMO

RESEARCH QUESTION: Are maternal depression and/or anxiety disorders (MDAD) before and during pregnancy associated with IVF outcomes? DESIGN: A total of 5661 women starting their first IVF cycle between 15 August 2014 and 31 December 2015 were pooled from a prospective cohort of IVF-conceived children. The self-rating depression scale (SDS) and self-rating anxiety scale (SAS) were used to determine MDAD. IVF outcomes were compared between MDAD+ and MDAD- groups. RESULTS: A total of 10.3% (572/5556) of women had MDAD before IVF (bMDAD). The fertilization rate was lower in the bMDAD+ group (59.41 ± 22.11% versus 61.72 ± 22.18%, Padjust < 0.05). No difference was found in the other IVF outcomes. Pregnancy and neonatal outcomes in women with singleton live births were similar between the two groups. A total of 17.4% (347) women with singleton live births had MDAD during the first trimester (pMDAD). Birthweight (3383 ± 556 g versus 3447 ± 518 g, Padjust < 0.05) was lower and incidence of low birthweight (LBW) (6.9% versus 3.3%, Padjust < 0.01) was higher in the pMDAD group. After adjustment for potential confounders (gestational age, maternal age, maternal pre-pregnancy body mass index, threatened abortion, hypertensive disorder complicating pregnancy and gestational diabetes mellitus), pMDAD remained significantly associated with LBW (odds ratio [OR] 2.50, 95% confidence interval [CI] 1.16-5.42, Padjust < 0.05). The preconception psychological state in the pMDAD group did not demonstrate any additional impact on neonatal outcomes. CONCLUSIONS: MDAD during the first trimester is associated with increased risk of LBW in offspring, whether preconception MDAD exists or not.

6.
Reprod Biol Endocrinol ; 17(1): 58, 2019 Jul 16.
Artigo em Inglês | MEDLINE | ID: mdl-31311571

RESUMO

BACKGROUND: To determine the effects of age and the serum anti-Müllerian hormone (AMH) level on in vitro fertilization (IVF) outcomes, especially among young women with low serum AMH levels and older women with high AMH levels. METHODS: This study was a cohort study in which a total of 9431 women aged 20-51 years who were undergoing their first IVF cycles were recruited. Ovarian response parameters included the number of retrieved oocytes, the number of 2 pronuclear zygotes (2PN), and the frequency of good-quality embryos (GQE). Pregnancy outcomes included the clinical pregnancy rate (CPR), live birth rate (LBR), miscarriage rate (MR), and cumulative CPR and LBR (CCPR and CLBR). RESULTS: Among women under 35 years of age, the ovarian response, CPR, CCPR, LBR and CLBR (p < 0.01) were significantly lower in the low-AMH group than in the average-AMH and high-AMH groups. In women above 35 years of age, the ovarian response, CPR, CCPR and CLBR (p < 0.01) were significantly higher in the average-AMH and low-AMH groups. The LBR in the older high-AMH group was significantly higher (37.45% vs 20.34%, p < 0.01) than that in the older low-AMH group, but there was no difference (37.45% vs 32.46%, p = 0.11) compared with the older average-AMH group. When there was a discrepancy between age and the AMH level, the young low-AMH group showed a poorer ovarian response but a better CPR (58.01% vs 49.44%, p < 0.01) and LBR (48.52% vs 37.45%, p < 0.01) than the older high-AMH group. However, the CCPR (65.37% vs 66.11%, p = 0.75) and CLBR (56.35% vs 52.89%, p = 0.15) between the two groups were comparable. The conservative CLBR in the two discrepancy groups increased until the third embryo transfer and reached a plateau thereafter. CONCLUSION(S): Even with a relatively low AMH level, young women still had better pregnancy outcomes following IVF than older women. However, increasing the AMH level improves the cumulative outcomes of the older group to a comparable level through a notable and superior ovarian response.


Assuntos
Hormônio Antimülleriano/sangue , Fertilização In Vitro , Idade Materna , Adulto , Fatores Etários , Estudos de Coortes , Transferência Embrionária , Feminino , Humanos , Pessoa de Meia-Idade , Recuperação de Oócitos , Gravidez , Resultado da Gravidez , Taxa de Gravidez
7.
Environ Pollut ; 247: 824-831, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30731307

RESUMO

Perfluoroalkyl substances (PFASs) are a family of synthetic, fluorinated organic compounds. They have been widely used in industrial applications and consumer products and widespread in the environment, wildlife and human. Experimental and epidemiologic evidence suggested that PFASs are capable of interfering with endocrine processes and have potential reproductive and developmental toxicities. Polycystic ovarian syndrome (PCOS), one of the main reasons of female infertility, is a common endocrine disorder in reproductive age women. We performed a case-control study to evaluate associations between PCOS-related infertility and PFASs concentrations in plasma. A total of 180 infertile PCOS-cases and 187 healthy controls were recruited from the Center for Reproductive Medicine of Shandong University. Blood specimens were collected at enrollment and analyzed for ten PFASs using liquid chromatography-tandem mass spectrometry. Multivariable logistic regression procedure was used to estimate odds ratios (ORs) and 95% confidence intervals (CIs) for each PFAS. Perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) were the dominant PFASs in the plasma of participants, with the median concentration of 5.07 ng/mL and 4.05 ng/mL, respectively. The median levels of individual PFAS were not significantly different between PCOS-cases and controls. While adjusted for the potential confounders (age, BMI, household income, education level, employment status, age at menarche, menstrual volume), the plasma concentration of perfluorododecanoic acid (PFDoA), a 12 carbons lengths of perfluorocarboxylic acids, was associated with a significantly increased risk of PCOS-related infertility (medium vs low tertile: OR = 2.36, 95% CI: 1.12, 4.99, P = 0.02; high vs low tertile: OR = 3.04, 95% CI: 1.19, 7.67, P = 0.02), with the P trend 0.01. No significant relationship was observed between PCOS-related infertility and other PFAS analytes in the adjusted model, despite perfluoroundecanoic acid showed a negative association (P trend 0.03). The potential reproductive health effects of PFASs and the underlying mechanisms merit further investigation in the future.


Assuntos
Exposição Ambiental/estatística & dados numéricos , Poluentes Ambientais/metabolismo , Fluorcarbonetos/metabolismo , Infertilidade Feminina/epidemiologia , Síndrome do Ovário Policístico/epidemiologia , Adulto , Ácidos Alcanossulfônicos , Caprilatos , Estudos de Casos e Controles , China/epidemiologia , Poluentes Ambientais/sangue , Ácidos Graxos , Feminino , Fluorcarbonetos/química , Humanos , Ácidos Láuricos , Modelos Logísticos , Razão de Chances , Reprodução
8.
Fertil Steril ; 111(1): 168-177, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30611404

RESUMO

OBJECTIVE: To explore metabolic disturbances in nonobese women with polycystic ovary syndrome (PCOS) compared with nonobese healthy controls. DESIGN: Systematic review and meta-analysis. SETTING: Not applicable. PATIENT(S): Nonobese women with PCOS and nonobese healthy controls. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Prevalence of metabolic disturbances including hyperinsulinemia, insulin resistance (IR), impaired fasting glucose (IFG), impaired glucose intolerance (IGT), prediabetes, dyslipidemia, hypercholesterolemia, hypertriglyceridemia, and low high-density lipoprotein (low-HDL), as well as other metabolic outcomes such as type 2 diabetes mellitus (T2DM), hypertension, metabolic syndrome (Mets), myocardial infarction, stroke, cerebrovascular accident, arterial occlusive disease, and coronary heart disease. RESULT(S): Compared to nonobese controls, nonobese women with PCOS showed a higher prevalence of hyperinsulinemia (odds ratio [OR], 36.27; 95% confidence interval [CI] 1.76-747.12), IR (OR, 5.70; 95% CI 1.46-22.32), IGT (OR, 3.42; 95% CI 1.56-7.52), T2DM (OR, 1.47; 95% CI 1.11-1.93), hypertriglyceridemia (OR, 10.46; 95% CI 1.39-78.56), low-HDL (OR, 4.03; 95% CI 1.26-12.95), and Mets (OR, 2.57; 95% CI 1.30-5.07). No significant difference was observed for IFG, pre-DM, dyslipidemia, hypercholesterolemia, and hypertension. In subgroup analysis, Whites exhibited increased risks of IR, IGT, IFG, T2DM, hypertension, and Mets, whereas no significant metabolic change was found in Asians. No study reported specifically an incidence of myocardial infarction, stroke, cerebrovascular accident, arterial occlusive disease, and coronary heart disease in nonobese women with PCOS. CONCLUSION(S): Nonobese women with PCOS also suffer from metabolic disturbances and the risk of long-term metabolic complications. Further efforts should be made to elucidate underlying mechanisms and possible interventions in the early phase.


Assuntos
Síndrome Metabólica/epidemiologia , Síndrome Metabólica/metabolismo , Síndrome do Ovário Policístico/epidemiologia , Síndrome do Ovário Policístico/metabolismo , Feminino , Humanos , Síndrome Metabólica/diagnóstico , Síndrome do Ovário Policístico/diagnóstico
9.
Glycobiology ; 29(1): 85-93, 2019 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-30239701

RESUMO

Charcot-Leyden crystal protein/Gal-10, abundantly expressed in eosinophils and basophils, is related to several immune diseases. Recently, crystallographic and biochemical studies showed that Gal-10 cannot bind lactose, because a glutamate residue (Glu33) from another monomer blocks the binding site. Moreover, Gal-10 actually forms a novel dimeric structure compared to other galectins. To investigate the role that Glu33 plays in inhibiting lactose binding, we mutated this residue to glutamine, aspartate, and alanine. The structure of E33A shows that Gal-10 can now bind lactose. In the hemagglutination assay, lactose could inhibit E33A from inducing chicken erythrocyte agglutination. Furthermore, we identified a tryptophan residue (Trp127) at the interface of homodimer that is crucial for Gal-10 dimerization. The variant W127A, which exists as a monomer, exhibited higher hemagglutination activity than wild type Gal-10. The solid phase assay also showed that W127A could bind to lactose-modified sepharose-6B, whereas wild type Gal-10 could not. This indicates that the open carbohydrate-binding site of the W127A monomer can bind to lactose. In addition, the distribution of EGFP-tagged Gal-10 and its variants in HeLa cells was investigated. Because Trp72 is the highly conserved in the ligand binding sites of galectins, we used EGFP-tagged W72A to show that Gal-10 could not be transported into the nucleus, indicating that Trp72 is crucial for Gal-10 transport into that organelle.


Assuntos
Núcleo Celular/metabolismo , Galectinas/metabolismo , Multimerização Proteica , Transporte Ativo do Núcleo Celular/fisiologia , Substituição de Aminoácidos , Núcleo Celular/genética , Cristalografia por Raios X , Galectinas/química , Galectinas/genética , Células HeLa , Humanos , Lactose/química , Lactose/genética , Lactose/metabolismo , Mutação de Sentido Incorreto , Domínios Proteicos , Especificidade por Substrato
10.
Rapid Commun Mass Spectrom ; 33(1): 74-80, 2019 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-30324637

RESUMO

RATIONALE: The combination of an Elemental Analyzer, a GasBench interface and Isotope Ratio Mass Spectrometry (EA-GasBench-IRMS system) is a promising method for the δ15 N analysis of samples containing trace amounts of nitrogen (N). Nevertheless, N blanks, which are limiting factors for the accuracy and precision of measured δ15 N values, have received little study. In this paper, a variety of N blank sources in the EA-GasBench-IRMS system were systematically evaluated in order to take effective measures to reduce the blank interference as much as possible. METHODS: N-Isotopic analysis was accomplished using an elemental analyzer coupled to an isotope ratio mass spectrometer via a sample loop and a GasBench interface. The N in the sample was converted into N2 gas in the EA system, and then transferred and trapped in a sample loop with a deactivated stainless-steel chromatography column packed with 5 A molecular sieve polymer at liquid nitrogen temperature (-196°C). Subsequently, the N2 gas was released by warming the sample loop up to 100°C and introduced into the isotope ratio mass spectrometer via the GasBench interface. The N blank sources in the EA-GasBench-IRMS system were investigated systematically by looking at seven parts: (a) Helium carrier gas, (b) Autosampler, (c) CO2 /water trap, (d) Size of reactor tube, (e) Sample collection time, (f) Oxygen gas, and (g) Capsules. RESULTS: The N blanks are mainly derived from the helium carrier gas, atmospheric N2 entrained into the system through the autosampler, and the N retained in the CO2 /water trap filled with CO2 absorbent and Mg(ClO4 )2 , which together can account for a total of ~507.3 nmol N. Through purifying the helium gas, modifying the autosampler and using a cryogenic trap, we reduced the N blank considerably, to ~10.7 nmol, and obtained a nearly uniform isotopic composition (δ15 NBlank = -4.54 ± 0.36‰ AIR, n = 32, 1SD) of blank N thus guaranteeing a reliable correction. CONCLUSIONS: Measurements on a set of IAEA-N1, IAEA-600 and collagen standards with 40 nmol-200 nmol N produced accurate δ15 N values with standard deviation of ±0.26‰, ±0.22‰ and ± 0.23‰ (1σ) after blank correction, respectively. Our findings offer clues to optimizing the analytical method for trace N isotopic determination and they are also beneficial to improving δ15 N measurement using conventional EA-IRMS.

11.
Biosci Rep ; 38(6)2018 12 21.
Artigo em Inglês | MEDLINE | ID: mdl-30413611

RESUMO

Placental protein 13/galectin-13 (Gal-13) is highly expressed in placenta, where its lower expression is related to pre-eclampsia. Recently, the crystal structures of wild-type Gal-13 and its variant R53H at high resolution were solved. The crystallographic and biochemical results showed that Gal-13 and R53H could not bind lactose. Here, we used site-directed mutagenesis to re-engineer the ligand binding site of wild-type Gal-13, so that it could bind lactose. Of six newly engineered mutants, we were able to solve the crystal structures of four of them. Three variants (R53HH57R, R53HH57RD33G and R53HR55NH57RD33G had the same two mutations (R53 to H, and H57 to R) and were able to bind lactose in the crystal, indicating that these mutations were sufficient for recovering the ability of Gal-13 to bind lactose. Moreover, the structures of R53H and R53HR55N show that these variants could co-crystallize with a molecule of Tris. Surprisingly, although these variants, as well as wild-type Gal-13, could all induce hemagglutination, high concentrations of lactose could not inhibit agglutination, nor could they bind to lactose-modified Sepharose 6b beads. Overall, our results indicate that Gal-3 is not a normal galectin, which could not bind to ß-galactosides. Lastly, the distribution of EGFP-tagged wild-type Gal-13 and its variants in HeLa cells showed that they are concentrated in the nucleus and could be co-localized within filamentary materials, possibly actin.


Assuntos
Galactosídeos/metabolismo , Galectinas/química , Galectinas/metabolismo , Lactose/metabolismo , Proteínas da Gravidez/química , Proteínas da Gravidez/metabolismo , Sítios de Ligação , Clonagem Molecular , Cristalografia por Raios X , Galectinas/análise , Galectinas/genética , Células HeLa , Humanos , Modelos Moleculares , Mutagênese Sítio-Dirigida , Proteínas da Gravidez/análise , Proteínas da Gravidez/genética
12.
Nature ; 558(7708): 68-72, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29849142

RESUMO

The fish-to-tetrapod transition-followed later by terrestrialization-represented a major step in vertebrate evolution that gave rise to a successful clade that today contains more than 30,000 tetrapod species. The early tetrapod Ichthyostega was discovered in 1929 in the Devonian Old Red Sandstone sediments of East Greenland (dated to approximately 365 million years ago). Since then, our understanding of the fish-to-tetrapod transition has increased considerably, owing to the discovery of additional Devonian taxa that represent early tetrapods or groups evolutionarily close to them. However, the aquatic environment of early tetrapods and the vertebrate fauna associated with them has remained elusive and highly debated. Here we use a multi-stable isotope approach (δ13C, δ18O and δ34S) to show that some Devonian vertebrates, including early tetrapods, were euryhaline and inhabited transitional aquatic environments subject to high-magnitude, rapid changes in salinity, such as estuaries or deltas. Euryhalinity may have predisposed the early tetrapod clade to be able to survive Late Devonian biotic crises and then successfully colonize terrestrial environments.


Assuntos
Evolução Biológica , Ecossistema , Vertebrados/classificação , Animais , Organismos Aquáticos/classificação , Osso e Ossos/química , Peixes/classificação , Água Doce/química , Isótopos/análise , Paleontologia , Filogenia , Água do Mar/química
13.
J Chromatogr A ; 1562: 47-58, 2018 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-29859683

RESUMO

An intermittent simulated moving bed (3F-ISMB) operation scheme, the extension of the 3W-ISMB to the non-linear adsorption region, has been introduced for separation of glucose, lactic acid and acetic acid ternary-mixture. This work focuses on exploring the feasibility of the proposed process theoretically and experimentally. Firstly, the real 3F-ISMB model coupled with the transport dispersive model (TDM) and the Modified-Langmuir isotherm was established to build up the separation parameter plane. Subsequently, three operating conditions were selected from the plane to run the 3F-ISMB unit. The experimental results were used to verify the model. Afterwards, the influences of the various flow rates on the separation performances were investigated systematically by means of the validated 3F-ISMB model. The intermittent-retained component lactic acid was finally obtained with the purity of 98.5%, recovery of 95.5% and the average concentration of 38 g/L. The proposed 3F-ISMB process can efficiently separate the mixture with low selectivity into three fractions.


Assuntos
Técnicas de Química Analítica/métodos , Ácido Láctico/isolamento & purificação , Ácido Acético/isolamento & purificação , Adsorção , Técnicas de Química Analítica/instrumentação , Glucose/isolamento & purificação
14.
Reprod Biomed Online ; 37(2): 178-183, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-29724534

RESUMO

RESEARCH QUESTION: Does an association exist between serum anti-Müllerian hormone (AMH) level, the marker of biological ovarian age, and embryonic aneuploidy risk in recurrent spontaneous miscarriage (RSM) patients of reproductive age? DESIGN: This retrospective study included a total of 422 IVF cycles of 394 unexplained RSM patients undergoing preimplantation genetic testing for aneuploidy (PGT-A), enrolled from January 2014 to December 2016. Subjects were divided into three groups according to the 25th (1.50 ng/ml) and 75th (5.60 ng/ml) percentiles of AMH level (Group 1: low AMH <1.50 ng/ml [N = 107], Group 2: normal AMH 1.50- < 5.60 ng/ml [N = 210] and Group 3: high AMH ≥ 5.60 ng/ml [N = 105]). RESULTS: There was a significant difference in embryonic aneuploid rate between AMH groups (66.7% versus 42.9% versus 50.0%, Groups 1 to 3, respectively, P = 0.006). It was significantly higher in the low AMH group (Group 1) compared with that in the normal AMH group (Group 2, P1vs2 = 0.002) and high AMH group (Group 3, P1vs3 = 0.015). After age stratification, embryonic aneuploidy rate was still significantly different among AMH groups with a similar trend in women ≥35 years old (68.2% versus 54.4% versus 51.0%, P = 0.038, P1vs2 = 0.025, P1vs3 = 0.035), but not in young subjects. CONCLUSIONS: These findings indicate that low AMH level was associated with increased risk of embryo aneuploidy only in women of advanced age. Maternal diminished ovarian reserve along with oocyte ageing may contribute to impaired chromosomal competence of the embryo.


Assuntos
Aneuploidia , Hormônio Antimülleriano/sangue , Testes Genéticos , Idade Materna , Diagnóstico Pré-Implantação , Adulto , Fatores Etários , Feminino , Fertilização In Vitro , Humanos , Reserva Ovariana , Gravidez , Estudos Retrospectivos , Fatores de Risco
15.
Carbohydr Polym ; 195: 593-600, 2018 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-29805016

RESUMO

Pectin, natural polysaccharide biopolymer, was chelated with cations (Mg2+/Ca2+) to form an interwoven framework. Herein, the graphene hydrogel electrodes were self-assembled by the synergistic effects of pectin-cations. The optimum combination proportion was determined, the Mg2+/Ca2+-pectin matrix cross-linked graphene hydrogel (Mg2+/Ca2+-PGH) electrodes exhibited a large specific capacitance of about 839.2 F g-1 with high coulombic efficiency of 191.8% at a current density of 1 A g-1. The assembled flexible supercapacitor displayed excellent stability (capacitance retention of 98.5% after 2000 charge/discharge cycles) and flexibility (the specific capacitance remained 98.4% of its original value after 500 folding/unfolding cycles). Such flexible and high-performance Mg2+/Ca2+-PGH electrodes are attractive in the field of lightweight, miniature and wearable energy storage devices.

16.
Glycobiology ; 28(3): 159-168, 2018 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-29293962

RESUMO

Galectin-10 (Gal-10) which forms Charcot-Leyden crystals in vivo, is crucial to regulating lymph cell function. Here, we solved the crystal structures of Gal-10 and eight variants at resolutions of 1.55-2.00 Å. Structural analysis and size exclusion chromatography demonstrated that Gal-10 dimerizes with a novel global shape that is different from that of other prototype galectins (e.g., Gal-1, -2 and -7). In the Gal-10 dimer, Glu33 from one subunit modifies the carbohydrate-binding site of another, essentially inhibiting disaccharide binding. Nevertheless, glycerol (and possibly other small hydroxylated molecules) can interact with residues at the ligand binding site, with His53 being the most crucial for binding. Alanine substitution of the conserved Trp residue (Trp72) that is crucial to saccharide binding in other galectins, actually leads to enhanced erythrocyte agglutination, suggesting that Trp72 negatively regulates Gal-10 ligand binding. Overall, our crystallographic and biochemical results provide insight into Gal-10 ligand binding specificity.


Assuntos
Carboidratos/química , Dimerização , Galectinas/química , Sítios de Ligação , Galectinas/genética , Galectinas/isolamento & purificação , Humanos , Ligantes , Modelos Moleculares
17.
Sci Rep ; 8(1): 980, 2018 01 17.
Artigo em Inglês | MEDLINE | ID: mdl-29343868

RESUMO

During pregnancy, placental protein-13 (galectin-13) is highly expressed in the placenta and fetal tissue, and less so in maternal serum that is related to pre-eclampsia. To understand galectin-13 function at the molecular level, we solved its crystal structure and discovered that its dimer is stabilized by two disulfide bridges between Cys136 and Cys138 and six hydrogen bonds involving Val135, Val137, and Gln139. Native PAGE and gel filtration demonstrate that this is not a crystallization artifact because dimers also form in solution. Our biochemical studies indicate that galectin-13 ligand binding specificity is different from that of other galectins in that it does not bind ß-galactosides. This is partly explained by the presence of Arg53 rather than His53 at the bottom of the carbohydrate binding site in a position that is crucial for interactions with ß-galactosides. Mutating Arg53 to histidine does not re-establish normal ß-galactoside binding, but rather traps cryoprotectant glycerol molecules within the ligand binding site in crystals of the R53H mutant. Moreover, unlike most other galectins, we also found that GFP-tagged galectin-13 is localized within the nucleus of HeLa and 293 T cells. Overall, galectin-13 appears to be a new type of prototype galectin with distinct properties.


Assuntos
Aminoácidos/química , Dissacarídeos/química , Galectinas/química , Glicerol/química , Monossacarídeos/química , Proteínas da Gravidez/química , Aminoácidos/metabolismo , Animais , Sítios de Ligação , Núcleo Celular/metabolismo , Núcleo Celular/ultraestrutura , Galinhas , Cristalografia por Raios X , Dissacarídeos/metabolismo , Galactosídeos , Galectinas/genética , Galectinas/metabolismo , Expressão Gênica , Glicerol/metabolismo , Proteínas de Fluorescência Verde/genética , Proteínas de Fluorescência Verde/metabolismo , Células HEK293 , Células HeLa , Testes de Hemaglutinação , Humanos , Ligação de Hidrogênio , Modelos Moleculares , Monossacarídeos/metabolismo , Mutação , Proteínas da Gravidez/genética , Proteínas da Gravidez/metabolismo , Ligação Proteica , Conformação Proteica em alfa-Hélice , Conformação Proteica em Folha beta , Domínios e Motivos de Interação entre Proteínas , Multimerização Proteica , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo
18.
Front Physiol ; 8: 208, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28428760

RESUMO

To understand dynamic changes in polyamines (PAs) forms and components of polyamine metabolism in zoysiagrass (Zoysia japonica Steud.) response to salt stress with exogenous spermidine (Spd) application, two Chinese zoysia cultivars, z081 and z057, were exposed to sodium chloride stress for 2, 4, 6, and 8 days. The z057 cultivar possesses higher salinity tolerance than the z081 cultivar. Salt stress decreased the zoysiagrass fresh weight (FW) and increased free Spd and spermine (Spm) levels and soluble and insoluble putrescine (Put), Spd and Spm levels in both cultivars. Moreover, salt stress enhanced the activities of arginine decarboxylase (ADC), ornithine decarboxylase (ODC), S-adenosylmethionine decarboxylase (SAMDC), and diamine oxidase (DAO). Exogenous Spd increased PA metabolism and ADC, SAMDC, and DAO activities and decreased free Put levels under salt stress conditions in both cultivars. In addition, structural equation modeling (SEM) showed that ODC, SAMDC, and DAO contributed to PA metabolism, and endogenous Spd levels also contributed to endogenous Spm levels. Free PAs may be the primary factor influencing the variation of other PA forms. SEM also indicated that ADC and polyamine oxidase (PAO) play a limited role in enhancing zoysia salt tolerance via PA metabolism under salt stress.

19.
Environ Sci Pollut Res Int ; 24(11): 10381-10390, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28281057

RESUMO

Few studies have explored the association between circulatory system diseases (CSDs) and provincial socioeconomic and environmental factors from spatial perspective, although large literature have focused on CSD. The numbers of death of hypertension disease (HD), ischemic heart disease (IHD), and cerebrovascular disease (CVD) are investigated, and 14 representative socioeconomic and environmental factors are collected. Stepwise regression model (SRM) and geographically weighted regression model (GWRM) are applied to determine the spatial correlation between the number of death of those diseases and selected factors. The results are the following: (1) diseases exhibit a pattern of zonal distribution. Higher HD is mostly distributed in south district, whereas higher IHD and CVD are observed in the north area. (2) SO2 emission amount (SO2 EA) is significantly positively related with HD, while coal consumption (CC) and PM2.5 are notably positively correlated with IHD and CVD. (3) A 10,000 tons increase in SO2 EA results in three increases in the numbers of death of HD. For every 100 ten thousand tons (TTTs) increase in CC, the death of IHD and CVD increases by 11.1 and 15.7, while for every 1 µg/m3 increase in ambient PM2.5 concentration, the numbers of death of IHD and CVD increase by 34.773 and 43.222, respectively. (4) Our findings show that there exist spatial differences for SO2 EA, CC, and PM2.5 influencing HD, IHD, and CVD. This study is expected to provide a reference for HD, IHD, and CVD control in different regions.


Assuntos
Doenças Cardiovasculares , Transtornos Cerebrovasculares , Sistema Cardiovascular , China , Humanos , Isquemia Miocárdica
20.
PLoS One ; 12(1): e0169837, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28121989

RESUMO

OBJECTIVE: To compare the risk of ectopic pregnancy (EP) after embryo transfer on day 3(D3-ET) and day 5(D5-ET). DESIGN: Meta-analysis. PATIENTS: Women with pregnancy resulting from in vitro undergoing in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). RESULT(S): Twenty-two studies were identified through research conducted using the PubMed, Embase, and Cochrane databases and ClinicalTrials.gov. All studies were conducted prior to October 2016. Adding the reproductive data from our center, a total of 143 643 pregnancies were reviewed(D3-ET: n = 62027,D5-ET:n = 81616). A lower EP rate was found in women undergoing D5-ET than in those undergoing D3-ET [relative risk (RR), 0.67;95% confidence interval (CI), 0.54-0.85;143643 pregnancies in 23 studies; I2 = 67%]. These results were validated in subgroups of fresh embryo-transfer (Fre-ET) cycles [RR, 0.78; 95%CI, 0.69-0.88; 91 871 pregnancies in 21 studies; I2 = 29%] and frozen-thawed embryo-transfer (Fro-ET) cycles [RR, 0.43; 95%CI, 0.36-0.51; 51 772 pregnancies in 10 studies; I2 = 33%]. After separating out the randomized controlled trials (RCTs), a significant difference was found in the retrospective studies in both subgroups [both Fre-ET (RR,0.78;95% CI 0.69-0.88);91182 pregnancies in 14 studies; I2 = 45%] and Fro-ET(RR,0.43;95% CI 0.36-0.51; 51751pregnancies in 9 studies;I2 = 33%)], while the RCTs showed no statistical significance for Fre-ET cycles[RR,0.86;95% CI 0.32-2.26); 689 pregnancies in 7 studies; I2 = 0%]. CONCLUSION(S): The present study indicates that D5-ET reduces the risk for EP in cycles that use IVF or ICSI, compared with D3-ET. It suggests that D5-ET may be a better choice for decreasing the EP rate in assisted reproductive technology. Further high-quality randomized controlled trials are anticipated.


Assuntos
Transferência Embrionária/métodos , Fertilização In Vitro/métodos , Gravidez Ectópica/epidemiologia , Transferência Embrionária/efeitos adversos , Feminino , Fertilização In Vitro/efeitos adversos , Humanos , Incidência , Gravidez , Taxa de Gravidez , Gravidez Ectópica/etiologia , Fatores de Risco , Fatores de Tempo
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