Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 81
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Nanotechnology ; 2020 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-32413888

RESUMO

Realizing high capacitance at high current densities is one of the challenges for battery electrode materials towards practical applications, especially for metal oxide electrode materials. By designing a specific structure that can alleviate volume expansion and accelerate the diffusion of the ions is an effective way to achieve this goal. Herein, a multilayer porous core-shell structured manganese cobalt oxide (MnCo2O4/C) was obtained by using a simple route that combines the hydrothermal method with calcination. The structure is similar to the Russian doll, which is nested by three to four layers of concentric porous shells. The porous multilayer core-shell structures can relieve volume expansion during discharge/charge, and also enable the diffusion path of lithium ions to be reduced. In addition, it can provide a richer active site. When used as an anode material, MnCo2O4/C showed satisfactory electrochemical performance. After 800 cycles at a current density of 1 A g- 1 a specific capacity of 978 mAh g-1 was able to be achieved. Even at a high current density of 10 A g-1, the electrode could still deliver a specific capacity of 251 mAh g-1, which made it more likely for powering large equipment such as electric vehicles.

2.
Zhonghua Wei Chang Wai Ke Za Zhi ; 23(3): 289-293, 2020 Mar 25.
Artigo em Chinês | MEDLINE | ID: mdl-32192309

RESUMO

Objective: To compared the short-term surgical outcomes of the vertical supraumbilical incision with the left lower oblique incision for specimen retrieval in laparoscopic resection for rectal cancer. Methods: A retrospective cohort study was performed. Inclusion criteria: (1) rectal cancer confirmed by colonoscopy and pathological examination; (2) undergoing the operation for the first time; (3) laparoscopic rectal surgery performed by the same surgeon team; (4) age of > 18 years and < 76 years old. According to above criteria, clinical data of 178 consecutive patients scheduled for laparoscopic surgery for rectal cancer at Department of Gastrointestinal Surgery of Renji Hospital between March 2015 and December 2017 were collected. Based on incision site of the mini-laparotomy, patients were classified to the vertical supraumbilical incision group (n=75) and the left lower oblique incision group (n=103). There were no significant differences in baseline data, such as age, gender, body mass index (BMI), tumor diameter, preoperative carcinoembryonic antigen (CEA) level, score of American Society of Anesthesiologists, TNM stage, between the two groups (all P>0.05). Perioperative variables and follow-up data were compared between two groups. Results: Between the vertical supraumbilical incision group and the left lower oblique incision group, the operation time [(131.7±3.7) minutes vs. (138.5±3.5) minutes], operative bleeding volume [(138.9±11.5) ml vs. (154.3±10.3) ml], length of auxiliary incision [(4.0±0.1) cm vs. (4.0±0.1) cm], and distance from anastomosis to dentate line [(3.8±0.1) cm vs. (4.2±0.1) cm] were not significantly different (all P>0.05). As compared to the left lower oblique incision group, patients in vertical supraumbilical incision group had earlier flatus [(62.7±2.3) hours vs. (69.2±1.7) hours, t=2.282, P=0.023], earlier ambulation [(41.9±1.8) hours vs. (46.78±1.42) hours, t=2.131, P=0.032], lower pain VAS scores at postoperative 24 hours (2.0±0.1 vs. 2.4±0.1, t=2.172, P=0.032) and 48 hours (2.7±0.1 vs. 3.0±0.1, P<0.05), and lower incidence of postoperative incisional hernia [6.7% (5/75) vs. 9.7% (10/103), χ(2)=3.942, P=0.042]. However, the postoperative fluids intake time, hospitalization days, pain VAS scores at postoperative 12 hours and postoperative complications (wound infection, anastomotic leakage, urinary retention, intestinal obstruction) were not significantly different between the two groups (all P>0.05). Conclusion: The vertical supraumbilical incision in laparoscopic resection for rectal cancer can reduce the degree of postoperative pain, facilitate early recovery of intestinal function and decrease the incidence of incisional hernia.


Assuntos
Laparoscopia , Neoplasias Retais , Ferida Cirúrgica , Humanos , Duração da Cirurgia , Neoplasias Retais/cirurgia , Estudos Retrospectivos , Resultado do Tratamento
3.
Osteoporos Int ; 31(6): 1135-1143, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32157326

RESUMO

It is unknown whether a relationship exists between bone mineral density (BMD) and atherosclerosis with or without vascular calcification. In our study, a negative correlation between carotid atherosclerosis and BMD was found in female T2DM patients with vascular calcification, but not in those without calcification and males. INTRODUCTION: Atherosclerosis is considered associated with low bone mineral density (BMD). However, most previous studies focus on patients with arterial atherosclerosis with vascular calcification. It is still unknown whether a relationship exists between atherosclerosis and BMD in patients without calcification. It is also unknown if sex plays a role in this relationship. METHODS: We performed a retrospective cross-sectional study, which included 1459 type 2 diabetes mellitus (T2DM) patients (648 males ≥ 50 years old, and 811 postmenopausal females). They were assigned to three groups: group 1 (patients without carotid plaques and without carotid calcification), group 2 (patients with carotid plaques but without carotid calcification), and group 3 (patients with carotid plaques and with carotid calcification). Clinical characteristics and BMD were compared. The relationship between atherosclerosis and BMD was determined by binary logistic regression analysis. Statistical analysis was performed using SPSS 25.0. RESULTS: Significant differences were only observed in women. The percentage of osteoporosis was higher in group 3 (43.64%) than in groups 1 (34.82%) and 2 (32.14%) (P = 0.016). Low BMD was found in the lumbar (P = 0.032), hip (P < 0.001), and femoral neck (P < 0.001). The odds ratio for osteoporosis increased significantly in a score-dependent manner in postmenopausal female patients with calcified atherosclerosis, but not in uncalcified patients. In men, no differences or relationships were identified. CONCLUSION: A negative correlation between carotid atherosclerosis and BMD was found in female T2DM patients with vascular calcification, but not in those without calcification. A similar relationship was not observed in male patients with or without calcification. Thus, the relationship between atherosclerosis and bone mineral density in patients with type 2 diabetes depends on vascular calcifications and sex.

4.
Zhonghua Yan Ke Za Zhi ; 55(7): 515-521, 2019 Jul 11.
Artigo em Chinês | MEDLINE | ID: mdl-31288355

RESUMO

Objective: To compare biometry with new biometers of Pentacam AXL and IOLMaster 700 and the widely used biometer of IOLMaster 500 in cataract patients. Methods: Cross-sectional study. A total of 223 eyes of 147 cataract patients from Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University during 4-8 December 2017 were recruited. There were 67 males and 80 females with an age of (64±11) years.The axial length (AL), mean keratometry (Km), anterior chamber depth (ACD) and white-to-white corneal diameter (WTW) from each device were recorded. The difference and agreement between the measurements were evaluated by ANOVA, LSD test, intraclass correlation coefficient and Bland-Altman analysis. Results: The success rate of AL measurement was 85.2% (190/223) with the Pentacam AXL, 96.9% (216/223) with the IOLMaster 700 and 90.6% (202/223) with the IOLMaster 500. There was no difference among devices in measuring the AL, Km and ACD (all P>0.05). Significant difference was found in WTW [(11.36±0.42) vs. (11.69±0.45) vs. (11.45±0.42) mm; F=34.696, P=0.000]. Intraclass correlation coefficient was 0.859-1.000, and Bland-Altman analysis showed good agreement among three devices. Conclusions: The new biometers of Pentacam AXL and IOLMaster 700 and the widely used IOLMaster 500 show no difference in measuring AL, Km and ACD. All three devices show good agreement in biometry of cataract patients. (Chin J Ophthalmol, 2019, 55: 515-521).


Assuntos
Câmara Anterior , Catarata , Idoso , Biometria , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Reprodutibilidade dos Testes
5.
Zhonghua Yan Ke Za Zhi ; 54(11): 843-848, 2018 Nov 11.
Artigo em Chinês | MEDLINE | ID: mdl-30440156

RESUMO

Objective: To analyze the ultrasound diagnosis and differential diagnosis of medium and small choroidal melanomas. Methods: It was a retrospective case series study. Thirty-six cases (36 eyes) with medium or small choroidal melanoma were collected between January 2016 and January 2017 in Beijing Tongren Hospital, including 16 males and 20 females aged (50±12) years old. All tumors' apical heights were within 5 mm and the largest basal diameter was within 15 mm. Sixty-nine cases (69 eyes) with choroidal hemangioma, including 45 males and 24 females aged (45±11) years old, and 16 cases (19 eyes) with choroidal metastasis, including 4 males and 12 females aged (50±11) years old, of equal size were also analyzed as differential diagnosis. Univariate (Chi-square test, Fisher's exact test, and analysis of variance) and multivariate analyses (Logistic regression) were used for statistical analysis. Results: There were significant differences in morphology, height, base-height ratio, internal echo, choroidal excavation and secondary retinal detachment among the three types of tumors(all P<0.05). Approximately 91.7% (33/36) of choroidal melanomas and 94.2% (65/69) of hemangiomas were flat or dome, while 63.2% (12/19) of metastatic tumors showed a polygonal shape and an irregular surface contour. The average height was (4.02±0.70) mm for choroidal melanoma, (3.10±1.08) mm for metastasis and (2.75±0.87) mm for hemangioma. The base-height ratio of choroidal melanoma, metastasis, and hemangioma was 2.62±0.48, 4.09±1.18 and 3.16±0.58, respectively. About 86.1% (31/36) of choroidal melanomas and 84.2% (16/19) of metastases showed lower echo than adjacent orbital tissue, while 92.8% (64/69) of hemangiomas had similar or higher echo. About 16.7% (6/36) of choroidal melanomas showed choroidal excavation, but no such signs were found in other tumors. Approximately 80.6% (29/36) of choroidal melanomas, 78.9% (15/19) of metastases and 52.2% (36/69) of hemangiomas showed secondary retinal detachment. The internal echo, height and base-height ratio were taken as significant independent influencing factors into the Logistic regression model. The low internal echo and large height were risk factors of choroidal melanoma (OR=0.016, 0.199, both P<0.05), while the low echo and large base-height ratio were risk factors of choroidal metastasis (OR=0.011, 0.073, both P<0.05). Conclusion: Ultrasonography plays an important role in the diagnosis of medium and small choroidal melanomas, and the echo, height, and base-height ratio of the lesion can be helpful for the diagnosis and differential diagnosis. (Chin J Ophthalmol, 2018, 54: 843-848).


Assuntos
Neoplasias da Coroide , Melanoma , Adulto , Neoplasias da Coroide/diagnóstico por imagem , Diagnóstico Diferencial , Feminino , Humanos , Masculino , Melanoma/diagnóstico por imagem , Pessoa de Meia-Idade , Estudos Retrospectivos , Ultrassonografia
6.
Beijing Da Xue Xue Bao Yi Xue Ban ; 50(5): 792-796, 2018 Oct 18.
Artigo em Chinês | MEDLINE | ID: mdl-30337737

RESUMO

OBJECTIVE: To establish a high performance liquid chromatography (HPLC) method for the determination of 8-methoxypsoralen (8-MOP) in mouse plasma and apply it to a pharmacokinetic study of 8-MOP. METHODS: 8-MOP was separated on a Waters Symmetry18 column (250 mm × 4.6 mm, 5 µm) and determined by HPLC using isocratic elution, and 5-methoxypsoralen was used as internal standard. The mobile phase consisted of methanol-water (55:45, V/V) at a flow rate of 1.0 mL/min. The excitation and emission wavelength of fluorescence detector were set at 334 nm and 484 nm respectively, and the internal standard method was used for quantitative analysis. In the study, 60 healthy ICR male mice were randomly divided into twelve groups. The mice in control group were administered intragastrically with 1% Tween 80, and the mice in the other eleven groups were administered intragastrically with 8-MOP (40 mg/kg). Plasma concentrations of 8-MOP in the mice at different time points after treatment were determined by HPLC. Pharmacokinetic parameters were calculated by DAS 2.0 software. RESULTS: The calibration curve of 8-MOP was linear with a correlation coefficient of 0.999 3 over the concentration range of 0.05 to 10 mg/L, and the limit of detection was 0.015 mg/L. The average recoveries of 8? MOP at three different concentrations (0.10, 0.50, 2.5 mg/L) were from 92.5% to 100.6%. The intra-day precision of 8-MOP was from 3.3% to 8.2%, while the inter-day precision was from 3.4% to 6.7% at three spiked concentration levels. The extraction recoveries of 8-MOP were from 90.9% to 92.0%, and the plasma samples could be stored at -80°C for 15 days at least at three spiked concentration levels. 8-MOP could be detected in mouse plasma 5 min after intragastrical administration to the mice (1.4 mg/L). The concentration of 8-MOP in the mouse plasma reached a maximum 2 h after administration, and 8-MOP could still be detected 24 h after administration (1.1 mg/L). t1/2 was (39.21±3.65) h, Cmax was (2.31±0.02) mg/L, tmax was (2.00±0.00) h, and AUC0-t was (33.34±1.19) (h×mg)/L. CONCLUSION: The proposed method is accurate and simple,suitable for pharmacokinetics of 8-MOP in mice.


Assuntos
Cromatografia Líquida de Alta Pressão , Metoxaleno , Fármacos Fotossensibilizantes , Animais , Calibragem , Masculino , Metoxaleno/sangue , Metoxaleno/farmacocinética , Camundongos , Camundongos Endogâmicos ICR , Fármacos Fotossensibilizantes/sangue , Fármacos Fotossensibilizantes/farmacocinética , Plasma , Distribuição Aleatória
7.
Eur Rev Med Pharmacol Sci ; 22(18): 6035-6041, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-30280788

RESUMO

OBJECTIVE: To investigate the specific role of long non-coding RNA (lncRNA) SETD5-AS1 in regulating stroke development, and its underlying mechanism. MATERIALS AND METHODS: Middle cerebral artery occlusion (MCAO) model and OGD/R (oxygen-glucose deprivation/reoxygenation) model were constructed for exploring the mechanism of ischemia-reperfusion injury induced by ischemic stroke. SETD5-AS1 expression in brain tissues of ischemic stroke mice and control mice was detected by quantitative Real-time-polymerase chain reaction (qRT-PCR). Proliferation and apoptosis of N2a cells were detected after transfection of overexpression plasmid or siRNA SETD5-AS1. The downstream gene of SETP5-AS1 was predicted by Starbase and PTEN was screened out. Both mRNA and protein expressions of PTEN in MCAO model and OGD/R model were detected. Furthermore, the binding condition of SETD5-AS1 and PTEN was verified by dual-luciferase reporter gene assay, RNA pull-down assay and RNA binding protein immunoprecipitation (RIP). The regulatory effect of SETD5-AS1 on PI3K/AKT pathway was detected by Western blot. RESULTS: SETD5-AS1 was highly expressed in the ischemia-reperfusion injury model. Overexpression of SETD5-AS1 in N2a cells resulted in increased apoptosis and decreased proliferation. PTEN expression was upregulated in MCAO model and OGD/R model. Dual-luciferase reporter gene assay indicated that SETD5-AS1 can promote PTEN transcription. The binding condition of SETD5-AS1 and PTEN was further verified by RNA pull-down assay and RIP. Overexpression of SETD5-AS1 in N2a cells inhibited PI3K/AKT pathway. CONCLUSIONS: SETD5-AS1 is highly expressed in the ischemia-reperfusion injury model. SETD5-AS1 participates in the development of ischemic stroke by activating PTEN and inhibiting PI3K/AKT pathway.


Assuntos
Infarto da Artéria Cerebral Média/genética , PTEN Fosfo-Hidrolase/genética , PTEN Fosfo-Hidrolase/metabolismo , RNA Longo não Codificante/genética , Acidente Vascular Cerebral/genética , Animais , Apoptose , Morte Celular , Linhagem Celular , Proliferação de Células , Modelos Animais de Doenças , Infarto da Artéria Cerebral Média/metabolismo , Masculino , Camundongos , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Transdução de Sinais , Acidente Vascular Cerebral/metabolismo , Regulação para Cima
8.
Zhonghua Yan Ke Za Zhi ; 54(9): 678-682, 2018 Sep 11.
Artigo em Chinês | MEDLINE | ID: mdl-30220183

RESUMO

Objective: To explore the difference and agreement in the measurement of anterior chamber depth (ACD) and anterior chamber angle data in age-related cataract patients between swept source anterior segment optical coherence tomography (SS-AS-OCT) and ultrasound biomicroscopy (UBM). Methods: A prospective study. Forty-five eyes from 45 age-related cataract patients were selected at Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University from November to December 2016. There were 23 males and 22 females aged (51±19) years. SS-AS-OCT and UBM were used to measure the ACD, angle opening distance (AOD 500) and trabecular-iris angle (TIA 500). The paired samples t-test and Pearson linear correlation were used for statistical analysis, and the Bland-Altman analysis was used to evaluate the measurement consistency of the two tools. Results: The ACD measured by SS-AS-OCT and UBM was (2.750±0.544) mm and (2.775±0.531) mm, respectively, the difference was statistically significant (t=2.109, P=0.040), and the measurement results were positively correlated (r=0.989, P<0.001). In the nasal, temporal, upper and lower positions, the AOD 500 and TIA 500 values measured by SS-AS-OCT and UBM had a significant difference [AOD 500: (0.451±0.201) mm vs.(0.421±0.194) mm, (0.499±0.229) mm vs. (0.465±0.207) mm, (0.390±0.215) mm vs. (0.355±0.208) mm, (0.441±0.226) mm vs. (0.423±0.215) mm, t=4.262, 4.350, 5.355, 4.415; TIA 500: 39.587°±14.698°vs. 36.944°±13.790°, 42.149°±14.138°vs. 40.289°±13.041°, 36.482°±15.887°vs. 35.600°±15.215°, 38.504°±14.805°vs. 36.818°±14.931°, t=5.131, 4.658, 4.174, 5.731; all P<0.001] and a significant linear correlation (AOD 500: r=0.971, 0.975, 0.979, 0.973; TIA 500: r=0.972, 0.957, 0.956, 0.970; all P<0.001). The Bland-Altman method showed that the two modalities had comparable results for the ACD, AOD 500 and TIA 500 (2.22%-6.67% of the measured value outside the 95% consistency boundaries). Conclusion: SS-OCT and UBM show a good agreement in the measurement of ACD and anterior chamber angle data in age-related cataract patients, though there is difference between them in measurement results. (Chin J Ophthalmol, 2018, 54: 678-682).


Assuntos
Catarata , Microscopia Acústica , Tomografia de Coerência Óptica , Adulto , Idoso , Câmara Anterior , Catarata/diagnóstico por imagem , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos
9.
Zhonghua Xue Ye Xue Za Zhi ; 39(7): 584-588, 2018 Jul 14.
Artigo em Chinês | MEDLINE | ID: mdl-30122019

RESUMO

Objective: To Evaluation the effect of PD-1 inhibitor Nivolumab on the proliferation and cytotoxicity of anti-CD19 chimeric antigen receptor T cells (CD19-CAR-T) in vitro. Methods: Five patients with high PD-1 expression in peripheral blood and five healthy volunteers were selected. These peripheral blood mononuclear cells were used as the source of T cells to prepare CD19-CAR-T cells. Different doses (72, 36, 18 µg/ml) of Nivolumab was added on day 8 to the culture medium. Patient T cells incubated with 72 µg/ml Nivolumab and CD19-CAR-T cells of healthy volunteers were used as controls. CCK-8, lactate dehydrogenase (LDH) cytotoxicity assay and ELASA were used to detect the proliferation capacity, the specific cytotoxicity and the inflammatory factor secretion. Results: ①T cells from patients with high expression of PD-1 as the source of CD19-CAR-T cells did not affect transfection rate compared with that of healthy volunteers [(32.80±7.22)% vs (35.10±5.84)%, t=-0.554, P=0.593]. ②Incubation of CD19-CAR-T cells with 72 µg/ml Nivolumab did not affect CD19-CAR-T cell proliferation, but its cytotoxicity was significantly higher than that of CD19-CAR-T cells alone or patients' T cells +72 µg/ml Nivolumab (all P<0.001), there was no significant difference in the killing activity between the 72 µg/ml and 36 µg/ml Nivolumab treated CD19-CAR-T cells on Pfeiffer cells (P=0.281, 0.267, respectively), and they were all higher than those of 18 µg/ml Nivolumab treated CD19-CAR-T cells (all P<0.001). ③Different doses of PD-1 inhibitor Nivolumab combined with CD19-CAR-T cells does not affect the secretion of IFN-γ and IFN-α (all P>0.05). Conclusion: Combination of 36 µg/ml PD-1 inhibitor and CD19-CAR-T cells could reduce the drug toxicity and enhance the cytotoxicity.


Assuntos
Nivolumabe/farmacologia , Antígenos CD19 , Proliferação de Células , Humanos , Leucócitos Mononucleares , Receptor de Morte Celular Programada 1 , Receptores de Antígenos de Linfócitos T , Receptores de Antígenos Quiméricos , Linfócitos T
10.
Zhonghua Yan Ke Za Zhi ; 54(3): 194-198, 2018 Mar 11.
Artigo em Chinês | MEDLINE | ID: mdl-29518878

RESUMO

Objective: To compare the significance of contrast-enhanced ultrasound (CEUS) and dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) in the diagnosis of uveal melanoma. Methods: It was a retrospective case series study. Sixty-three cases (63 eyes) of uveal melanoma patients (confirmed by histopathology) who underwent CEUS and DCE-MRI examination at Beijing Tongren Hospital during January 2010 and May 2014 were collected, including 30 males and 33 females, with average age of 47 years (ranged from 27 to 69 years). The data and images of CEUS and DCE-MRI were analyzed and the findings were reviewed and compared to pathology results so as to evaluate the diagnosis coincidence rate of using CEUS, DCE-MRI and both (combined CEUS and DCE-MRI). Fisher's exact test method was used to explore the efficiency of diagnosing uveal melanoma with CEUS, DCE-MRI and the two combined. Results: Among the 63 cases of uveal melanoma, the coincidence rates of CEUS, DCE-MRI and combined diagnosis for uveal melanoma were 93.7% (59/63), 90.5% (57/63) and 100% (63/63) respectively. There was no significant difference between CEUS and DCE-MRI (P=0.663). Fifty-five cases showed fast wash in and fast wash out, and 8 cases showed fast wash in and slow wash out in time-intensity curve of CEUS. Fifty-four cases showed rapid increase and rapid decrease, and 7 cases showed rapid increase and slow decrease, and 2 cases showed sustain enhancement in signal intensity-time curve of DCE-MRI. Conclusions: CEUS is an effective method in diagnosis of uveal melanoma. CEUS and DCE-MRI are of similar value in the diagnosis of uveal melanoma, physicians may be able to acquire more diagnostic information when using the two methods in combination.(Chin J Ophthalmol, 2018, 54: 194-198).


Assuntos
Imagem por Ressonância Magnética , Melanoma , Neoplasias Uveais , Adulto , Idoso , Meios de Contraste , Feminino , Humanos , Masculino , Melanoma/diagnóstico por imagem , Pessoa de Meia-Idade , Estudos Retrospectivos , Ultrassonografia , Neoplasias Uveais/diagnóstico por imagem
11.
Eur Rev Med Pharmacol Sci ; 22(2): 523-531, 2018 01.
Artigo em Inglês | MEDLINE | ID: mdl-29424913

RESUMO

OBJECTIVE: MiR-26a is involved in regulating myocardial remodeling and it is also related to organ fibrosis. Its role in myocardial fibrosis process is still controversy. As a definite target gene of miR-26a, phosphatase and tensin homology does on chromosome ten (PTEN) plays a role in regulating PTEN-PI3K/AKT signaling pathway. This study explored the function of miR-26a in regulating PTEN-PI3K/AKT signaling pathway, MMP-9 expression, and myocardial fibrosis after acute myocardial infarction (AMI). MATERIALS AND METHODS: AMI model was established on Sprague-Dawley (SD) rats. Hydroxyproline, COL1A1, miR-26a, PTEN, p-AKT, and MMP-9 expressions in myocardial tissue at 1 week, 2 weeks, and 4 weeks after modeling were detected. Human cardiac fibroblasts (HCF) were cultured in vitro to detect miR-26a, PTEN, p-AKT, MMP-9, COL1A1, and α-SMA expressions in the process of myofibroblast differentiation (P1, P3, P5). HCF in P5 were transfected with miR-26a mimics or inhibitor to test miR-26a, PTEN, p-AKT, MMP-9, COL1A1, and α-SMA expressions. RESULTS: Hydroxyproline, COL1A1, miR-26a, p-AKT, and MMP-9 overexpressed, while PTEN downregulated in myocardial tissue during the process of myocardial fibrosis after AMI. MiR-26a, PTEN, p-AKT, MMP-9, COL1A1, and α-SMA expression gradually enhanced, while PTEN declined, following the process of HCF differentiating into myofibroblast. MiR-26a elevation suppressed PTEN expression, and increased p-AKT, MMP-9, COL1A1, and α-SMA levels. MiR-26a reduction significantly upregulated PTEN level, weakened PI3K/AKT signaling pathway activity, and declined MMP-9, COL1A1, and α-SMA protein expression. CONCLUSIONS: MiR-26a upregulation may play a role in myocardial fibrosis after AMI by suppressing PTEN, enhancing PI3K/AKT signaling pathway and MMP-9 levels.


Assuntos
MicroRNAs/metabolismo , Infarto do Miocárdio/patologia , PTEN Fosfo-Hidrolase/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Animais , Antagomirs/metabolismo , Diferenciação Celular , Modelos Animais de Doenças , Fibroblastos/citologia , Fibroblastos/metabolismo , Hidroxiprolina/metabolismo , Masculino , Metaloproteinase 9 da Matriz/metabolismo , MicroRNAs/antagonistas & inibidores , MicroRNAs/genética , Infarto do Miocárdio/metabolismo , Miocárdio/citologia , Miocárdio/metabolismo , Ratos , Ratos Sprague-Dawley , Transdução de Sinais , Regulação para Cima
12.
Zhonghua Yan Ke Za Zhi ; 53(11): 842-846, 2017 Nov 11.
Artigo em Chinês | MEDLINE | ID: mdl-29141389

RESUMO

Objective: To examine the features and values of ultrasonic diagnosis for retinal detachment in eyes with silicone oil tamponade (SOT). Methods: A prospective study. A total of 257 eyes (257 patients) were filled with silicone oil between December 1, 2015 and August 1, 2016 in the Department of Ophthalmology at a hospital in Beijing. All of the patients underwent a color Doppler ultrasound examination in the supine and sitting positions a week prior to surgery. The pre-surgery conditions were compared with the subsequent observations during the surgery to analyze the features and values of ultrasonic diagnosis for retinal detachment in eyes with SOT. Results: Ultrasound manifestations of eyes with SOT included pseudo extension of the ocular axis and an arc-shaped echo between the eye wall and silicone oil. Diagnostic accuracy was 85.6% in the supine position and 93.8% in the sitting position. Conclusion: Ultrasound diagnosis for retinal detachment in eyes with SOT manifests itself in certain patterns. Compared with the traditional ultrasound examination in the supine position, the sitting position examination can be used to further increase the diagnostic accuracy in the ultrasound diagnosis for retinal detachment in eyes with SOT, providing reliable evidence for clinical and differential diagnosis. (Chin J Ophthalmol, 2017, 53: 842-846).


Assuntos
Descolamento Retiniano , Óleos de Silicone , Humanos , Estudos Prospectivos , Descolamento Retiniano/diagnóstico por imagem , Ultrassonografia , Acuidade Visual , Vitrectomia
13.
Zhonghua Gan Zang Bing Za Zhi ; 25(4): 246-248, 2017 Apr 20.
Artigo em Chinês | MEDLINE | ID: mdl-28494540

RESUMO

Hepatorenal syndrome (HRS) is functional renal injury in patients with liver cirrhosis or fulminant liver failure and is one of the complications of progressive liver failure which threatens patients' lives. With the progression of liver diseases, hemodynamic disturbance may eventually cause HRS. Achievements have been made in the pathophysiology, diagnostic criteria, and treatment of HRS. This article introduces the research advances in the pathophysiology, diagnosis, and treatment of HRS.


Assuntos
Síndrome Hepatorrenal/diagnóstico , Síndrome Hepatorrenal/terapia , Cirrose Hepática/fisiopatologia , Progressão da Doença , Síndrome Hepatorrenal/fisiopatologia , Humanos , Cirrose Hepática/complicações , Cirrose Hepática/terapia , Falência Hepática Aguda , Transplante de Fígado
14.
BJOG ; 124(7): 1028-1037, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28128524

RESUMO

Uterine leiomyosarcoma (LMS) are rare but aggressive tumours with poor clinical outcomes regardless of stage. Most tumours are identified by histopathology at time of surgery, and pre-operative diagnosis remains a clinical challenge. Management of early-stage LMS relies on surgical resection. Cytotoxic chemotherapy remains the mainstay of therapy for advanced-stage, recurrent or metastatic LMS, and includes single or combination doxorubicin-, ifosfamide- or gemcitabine-based regimens. Recent interest in genetic biomarkers led to developments of targeted therapies for LMS, although more research is needed to understand the molecular complexities underlying LMS to guide the development of novel treatment strategies. TWEETABLE ABSTRACT: The diagnosis and treatment of uterine LMS is challenging. Novel biomarkers offer hope for future therapies.


Assuntos
Biomarcadores Tumorais/genética , Leiomiossarcoma/terapia , Terapia de Alvo Molecular/métodos , Neoplasias Uterinas/terapia , Antineoplásicos/uso terapêutico , Antineoplásicos Hormonais/uso terapêutico , Feminino , Predisposição Genética para Doença , Humanos , Leiomiossarcoma/genética , Biologia Molecular , Mutação , Neoplasias Uterinas/genética , Útero/patologia
16.
Oncogene ; 36(9): 1245-1255, 2017 03 02.
Artigo em Inglês | MEDLINE | ID: mdl-27546620

RESUMO

Liver kinase B1 (LKB1) is mutationally inactivated in Peutz-Jeghers syndrome and in a variety of cancers including human papillomavirus (HPV)-caused cervical cancer. However, the significance of LKB1 mutations in cervical cancer initiation and progress has not been examined. Herein, we demonstrated that, in mouse embryonic fibroblasts, loss of LKB1 and transduction of HPV16 E6/E7 had an additive effect on constraining cell senescence while promoting cell proliferation and increasing glucose consumption, lactate production and ATP generation. Knockdown of LKB1 increased and ectopic expression of LKB1 decreased glycolysis, anchorage-independent cell growth, and cell migration and invasion in HPV-transformed cells. In the tumorigenesis and lung metastasis model in syngeneic mice, depletion of LKB1 markedly increased tumor metastatic colonies in lungs without affecting subcutaneous tumor growth. We showed that HPV16 E6/E7 enhanced the expression of hexokinase-ll (HK-II) in the glycolytic pathway through elevated c-MYC. Ectopic LKB1 reduced HK-II along with glycolysis. The inverse relationship between HK-II and LKB1 was also observed in normal and HPV-associated cervical lesions. We propose that LKB1 acts as a safeguard against HPV-stimulated aerobic glycolysis and tumor progression. These findings may eventually aid in the development of therapeutic strategy for HPV-associated malignancies by targeting cell metabolism.


Assuntos
Transformação Celular Neoplásica/metabolismo , Glucose/metabolismo , Glicólise/fisiologia , Infecções por Papillomavirus/metabolismo , Proteínas Serina-Treonina Quinases/metabolismo , Neoplasias do Colo do Útero/metabolismo , Neoplasias do Colo do Útero/patologia , Animais , Apoptose , Biomarcadores Tumorais/genética , Biomarcadores Tumorais/metabolismo , Estudos de Casos e Controles , Proliferação de Células , Transformação Celular Neoplásica/patologia , Feminino , Seguimentos , Hexoquinase/genética , Hexoquinase/metabolismo , Papillomavirus Humano 16/fisiologia , Humanos , Camundongos , Camundongos Endogâmicos C57BL , Estadiamento de Neoplasias , Proteínas Oncogênicas Virais/genética , Proteínas Oncogênicas Virais/metabolismo , Proteínas E7 de Papillomavirus/genética , Proteínas E7 de Papillomavirus/metabolismo , Infecções por Papillomavirus/patologia , Infecções por Papillomavirus/virologia , Prognóstico , Proteínas Serina-Treonina Quinases/genética , Proteínas Repressoras/genética , Proteínas Repressoras/metabolismo , Células Tumorais Cultivadas , Neoplasias do Colo do Útero/virologia
17.
Artigo em Inglês | MEDLINE | ID: mdl-27909789

RESUMO

Parameters such as the intensity of conditioned and unconditioned stimuli, the inter-trial interval, and starvation time can influence learning. In this study, the parameters that govern aversive learning in the oriental fruit fly, Bactrocera dorsalis, a serious pest of fruits and vegetables, were examined. Male flies were trained to associate the attractive odorant methyl eugenol, a male lure, with a food punishment, sodium chloride solution, and the conditioned suppression of the proboscis-extension response was investigated. We found that high methyl eugenol concentrations support a stronger association. With increasing concentrations of sodium chloride solution, a steady decrease of proboscis-extension response during six training trials was observed. A high level of learning was achieved with an inter-trial interval of 1-10 min. However, extending the inter-trial interval to 15 min led to reduced learning. No effect of physiological status (starvation time) on learning performance was detected, nor was any non-associative learning effect induced by the repeat presentation of odor or punishment alone. The memory formed after six training trials could be retained for at least 3 h. Our results indicate that aversive learning by oriental fruit flies can be affected by odor, punishment concentration and inter-trial interval.


Assuntos
Aprendizagem da Esquiva , Tephritidae , Animais , Eugenol/análogos & derivados , Privação de Alimentos , Masculino , Atividade Motora , Odorantes , Percepção Olfatória , Estimulação Física , Punição , Cloreto de Sódio , Fatores de Tempo
18.
Beijing Da Xue Xue Bao Yi Xue Ban ; 48(3): 398-402, 2016 Jun 18.
Artigo em Chinês | MEDLINE | ID: mdl-27318897

RESUMO

OBJECTIVE: To investigate the possible reversal effects of 1,3-diphenyl-1,3-propanedione (DPPD) for cocaine-induced content changes of neurotransmitters of brain in mice. METHODS: In this study, 36 healthy ICR male mice were randomly divided into control group, cocaine group, three DPPD pretreatment groups (200, 400, and 800 mg/kg) and DPPD alone group (800 mg/kg). The mice in control group were administered intragastrically with 1% Tween 80 for 3 d, and the mice in cocaine group were administered intragastrically with 1% Tween 80 for 2 d before cocaine was injected subcutaneously on the 3rd day. The mice in the three DPPD pretreatment groups were administered intragastrically (DPPD 200, 400, and 800 mg/kg) for 3 d before cocaine was injected subcutaneously 30 min after the administration on the 3rd day. The mice in DPPD alone group were administered intragastrically with DPPD at dose of 800 mg/kg for 3 d. The mice were sacrificed 20 minutes after cocaine injection. The contents of dopamine (DA) and 5-hydroxytryptamin (5-HT) in the mice brain were determined by high performance liquid chromatography (HPLC)-fluorescence detector, the contents of glutamic acid (Glu) and γ-aminobutyric acid (GABA) in the mice brain were determined by HPLC-ultraviolet detector, and the neurotransmitter levels were compared between the groups. RESULTS: The results showed that as compared with the control group, DA and GABA contents in cocaine group increased significantly (P<0.01 and P<0.05), while Glu content decreased (P<0.05). As compared with cocaine group, the DA levels in the three DPPD pretreatment groups (200, 400, and 800 mg/kg) all decreased significantly (P<0.01). In DPPD 200 mg/kg pre-administration group, GABA content decreased (P<0.05), and the contents of the four kinds of neurotransmitters had no statistical differences with those of the control group. CONCLUSION: DPPD may have potential reversal effects of the content changes of neurotransmitters in mice brain induced by cocaine at a lower dose.


Assuntos
Encéfalo/efeitos dos fármacos , Cocaína/farmacologia , Dopamina/química , Fenilenodiaminas/farmacologia , Serotonina/química , Animais , Química Encefálica , Ácido Glutâmico/química , Masculino , Camundongos , Camundongos Endogâmicos ICR , Neurotransmissores/química , Ácido gama-Aminobutírico/química
19.
Sci Rep ; 6: 25806, 2016 05 19.
Artigo em Inglês | MEDLINE | ID: mdl-27194388

RESUMO

Human respiratory syncytial virus (hRSV) is a leading cause of acute lower respiratory tract infection in infants, elderly and immunocompromised individuals. To date, no specific antiviral drug is available to treat or prevent this disease. Here, we report that the Smoothened receptor (Smo) antagonist cyclopamine acts as a potent and selective inhibitor of in vitro and in vivo hRSV replication. Cyclopamine inhibits hRSV through a novel, Smo-independent mechanism. It specifically impairs the function of the hRSV RNA-dependent RNA polymerase complex notably by reducing expression levels of the viral anti-termination factor M2-1. The relevance of these findings is corroborated by the demonstration that a single R151K mutation in M2-1 is sufficient to confer virus resistance to cyclopamine in vitro and that cyclopamine is able to reduce virus titers in a mouse model of hRSV infection. The results of our study open a novel avenue for the development of future therapies against hRSV infection.


Assuntos
Vírus Sincicial Respiratório Humano/fisiologia , Transcrição Genética , Proteínas Virais/metabolismo , Replicação Viral/fisiologia , Alcaloides/farmacologia , Animais , Linhagem Celular , RNA Polimerases Dirigidas por DNA/metabolismo , Modelos Animais de Doenças , Farmacorresistência Viral/efeitos dos fármacos , Feminino , Regulação Viral da Expressão Gênica/efeitos dos fármacos , Humanos , Camundongos Endogâmicos BALB C , Modelos Moleculares , Infecções por Vírus Respiratório Sincicial/tratamento farmacológico , Infecções por Vírus Respiratório Sincicial/virologia , Vírus Sincicial Respiratório Humano/efeitos dos fármacos , Receptor Smoothened/metabolismo , Transcrição Genética/efeitos dos fármacos , Veratrum/química , Alcaloides de Veratrum/química , Alcaloides de Veratrum/farmacologia , Alcaloides de Veratrum/uso terapêutico , Replicação Viral/efeitos dos fármacos , Replicação Viral/genética
20.
Exp Clin Endocrinol Diabetes ; 124(7): 452-6, 2016 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-27219888

RESUMO

PURPOSE: Animal studies suggested that there is an independent bone-osteocalcin-gonadal axis, except of the hypothalamic-pituitary-gonadal axis. Based on this hypothesis, the higher osteocalcin during the high bone turnover should be followed by higher testosterone formation. Yet such clinical evidence is limited. The patients with uncontrolled hyperthyroidism are proper model with high bone turnover. If this hypothesis is true, there should be high testosterone level in patients with uncontrolled hyperthyroidism. Therefore, Graves' disease patients were recruited to study the correlation between osteocalcin and testosterone. MATERIALS AND METHODS: 50 male hyperthyroidism patients with Graves' disease and 50 health persons matched by age and gender were enrolled in our cross-section study. Serum markers for thyroid hormone, sex hormone and bone metabolic markers including free triiodothyronine (FT3), free thyroxine (FT4), thyroid-stimulating hormone (TSH), testosterone, luteinizing hormone (LH), follicle-stimulating hormone (FSH) and osteocalcin (OC), C-terminal telopeptide fragments of type I collagen (CTX) were examined. The demographic parameters such as duration of disease were also collected. All data was analyzed by SPSS 20.0. RESULTS: High testosterone and osteocalcin level was observed in the hyperthyroidism patients (T 36.35±10.72 nmol/l and OC 46.79±26.83 ng/ml). In simple Pearson correlation, testosterone was positively associated with OC (r=0.486, P<0.001), and this positive relation still existed after adjusted for age, BMI, smoking, drinking, duration of disease, FT3, FT4, LH, FSH, CTX in multi-linear regression analysis (See Model 1-4). CONCLUSION: In male hyperthyroidism patients, osteocalcin was positively correlated with serum testosterone, which indirectly supports the hypothesis that serum osteocalcin participates in the regulation of sex hormone.


Assuntos
Remodelação Óssea/fisiologia , Hipertireoidismo/sangue , Osteocalcina/sangue , Testosterona/sangue , Adulto , Estudos Transversais , Humanos , Masculino , Pessoa de Meia-Idade
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA