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1.
Sci Total Environ ; 753: 141950, 2021 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-32906044

RESUMO

2,4-dichlorophenoxyacetic acid (2,4-D), a widely used herbicide, is a small organic chemical pollutant in the environment. To develop a nanobody-based immunoassay for monitoring trace levels of 2,4-D, a step-wise strategy for the generation of nanobodies highly specific against this small chemical was employed. Firstly, we synthesized three novel haptens mimicking 2,4-D and assessed their influence on the sensitivity and specificity of the existing antibody-based assay. Polyclonal antibodies (pAb) from rabbits showed good sensitivity and moderate specificity for 2,4-D, pAb from llama based on selected haptens showed similar performance when compared to those from rabbits. Secondly, nanobodies derived from llama were generated for 2,4-D by an effective procedure, including serum monitoring and one-step library construction. One nanobody, NB3-9, exhibited good sensitivity against 2,4-D (IC50 = 29.2 ng/mL) had better specificity than the rabbit pAb#1518, with no cross-reactivities against the 2,4-D analogs tested. Thirdly, one-step fluorescent enzyme immunoassay (FLEIA) for 2,4-D based on a nanobody-alkaline phosphatase (AP) fusion was developed with IC50 of 1.9 ng/mL and a linear range of 0.4-8.6 ng/mL. Environmental water samples were analyzed by FLEIA and LC-MS/MS for comparison, and the results were consistent between both methods. Therefore, the proposed step-wise strategy from hapten design to nanobody-AP fusion production was successfully conducted, and the resulting nanobody based FLEIA was demonstrated as a convenient tool to monitor 2,4-D residuals in the environment.


Assuntos
Herbicidas , Água , Ácido 2,4-Diclorofenoxiacético , Animais , Cromatografia Líquida , Ensaio de Imunoadsorção Enzimática , Herbicidas/análise , Coelhos , Espectrometria de Massas em Tandem
2.
Med Sci Monit ; 26: e924671, 2020 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-33077705

RESUMO

BACKGROUND Despite the promising results of immunotherapy in cancer treatment, new response patterns, including pseudoprogression and hyperprogression, have been observed. Radiomics is the automated extraction of high-fidelity, high-dimensional imaging features from standard medical images, allowing comprehensive visualization and characterization of the tissue of interest and corresponding microenvironment. This study assessed whether radiomics can predict response to immunotherapy in patients with malignant tumors of the digestive system. MATERIAL AND METHODS Computed tomography (CT) images of patients with malignant tumors of the digestive system obtained at baseline and after immunotherapy were subjected to radiomics analyses. Radiomics features were extracted from each image. The formula of the screened features and the final predictive model were obtained using the Least Absolute Shrinkage and Selection Operator (LASSO) algorithm. RESULTS Imaging analysis was feasible in 87 patients, including 3 with pseudoprogression and 7 with hyperprogression. One hundred ten radiomics features were obtained before and after treatment, including 109 features of the target lesions and 1 of the aorta. Four models were constructed, with the model constructed from baseline and post-treatment CT features having the best classification performance, with a sensitivity, specificity, and AUC of 83.3%, 88.9%, and 0.806, respectively. CONCLUSIONS Radiomics can predict the response of patients with malignant tumors of the digestive system to immunotherapy and can supplement conventional evaluations of response.

3.
Lupus ; 29(14): 1854-1865, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33028176

RESUMO

Although the original purpose of the systemic lupus erythematosus (SLE) classification criteria was to distinguish SLE from other mimic diseases, and to facilitate sample selection in scientific research, they have become widely used as diagnostic criteria in clinical situations. It is not known yet if regarding classification criteria as diagnostic criteria, what problems might be encountered? This is the first study comparing the three sets of classification criteria for SLE, the 1997 American College of Rheumatology (ACR'97), 2012 Systemic Lupus International Collaborating Clinics (SLICC'12) and 2019 European League Against Rheumatism/American College of Rheumatology (EULAR/ACR'19), for their ability to distinguish patients with SLE from patients with pure mucocutaneous manifestations (isolated cutaneous lupus erythematosus without internal disease, i-CLE) in the lupus disease spectrum. 1,865 patients with SLE and 232 patients with i-CLE were recruited from a multicenter study. We found that, due to low specificity, none of the three criteria are adept at distinguishing patients with SLE from patients with i-CLE. SLICC'12 performed best among the original three criteria, but if a positive ANA was removed as an entry criterion, EULAR/ACR'19 would performed better. A review of previous studies that compared the three sets of criteria was presented in this work.

4.
Am J Epidemiol ; 2020 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-33034339

RESUMO

Depression is a leading cause of disability in the United States, but its impact on mortality among racially diverse, low-socioeconomic populations is largely unknown. Using data from the Southern Community Cohort Study, 2002-2015, we prospectively evaluated the associations of depressive symptoms with all-cause and cause-specific mortality in 67,781 black (72.3%) and white (27.7%) adults, predominantly with a low-socioeconomic status. Baseline depressive symptoms were assessed using the ten-item Center for Epidemiological Studies Depression Scale. The median follow-up time was 10.0 years. Multivariate Cox regression was used to estimate hazard ratios (HRs) and 95% confidence intervals (CIs) for mortality in association with depressive symptoms. Mild/moderate/severe depressive symptoms were associated with increased all-cause (HR=1.12, 95%CI: 1.03-1.22; HR=1.17, 95%CI: 1.06-1.29; HR=1.15, 95%CI: 1.03-1.28, respectively) and cardiovascular disease mortality (HR=1.23, 95%CI: 1.05-1.44; HR=1.18, 95%CI: 0.98-1.42; HR=1.43, 95%CI: 1.17-1.75, respectively) in whites but not in blacks (Pinteraction< 0.001, for both). Mild/moderate/severe depressive symptoms were associated with increased external cause mortality in both races (HR=1.24, 95%CI: 1.05-1.46; HR=1.31, 95%CI: 1.06-1.61; HR=1.42, 95%CI: 1.11-1.81, respectively; for all subjects, Pinteraction=0.48). No association was observed for cancer mortality. Our study showed that depression-mortality associations differed by race and cause of death in individuals with a low-socioeconomic status.

5.
Curr Pharm Des ; 2020 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-32867648

RESUMO

MicroRNA (miRNA/miR) is a class of small evolutionarily conserved non-coding RNA, which can inhibit the target gene expression at post-transcriptional level and serve as significant roles in cell differentiation, proliferation, migration and apoptosis. Of note, the aberrant miR-21 has been involved in the generation and development of multiple lung diseases, and identified as a candidate of biomarker, therapeutic target, or indicator of prognosis. MiR-21 relieves acute lung injury via depressing the PTEN/Foxo1-TLR4/NF-κB signaling cascade,where as promotes lung cancer cell growth, metastasis, and chemo/radio-resistance by decreasing the expression of PTEN and PDCD4 and promoting the PI3K/AKT transduction.The purpose of this review is to elucidate the potential mechanisms of miR-21 associated lung diseases, with an emphasis on its dual regulating effects, which will trigger novel paradigms in the molecular therapy.

6.
Clin Respir J ; 2020 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-32931143

RESUMO

INTRODUCTION: Pulmonary embolism (PE) is a potentially fatal complication and its morbidity together with fatalness will further increase when in patients with malignant tumors. Fast and accurate early diagnosis of PE thus seems considerably important. OBJECTIVE: To explore the risk factors of lung cancer complicated with PE. MATERIALS AND METHODS: A retrospective cohort study consisted of 40 lung cancer patients with PE (PE group) and 60 lung cancer patients without PE (non-PE group) were analyzed. RESULTS: The white blood cell (WBC) count, D-dimer and low-density lipoprotein (LDL) were higher in PE group than those in non-PE group (P < 0.05), whereas the arterial partial pressure of oxygen (PaO2 ) in PE group was lower than that in non-PE group (P < 0.05). Carcinoembryonic antigen (CEA) level between two groups also exhibited statistical difference (P < 0.05). Those lung adenocarcinoma patients with stages III and IV tumor, coupled with deep venous thrombosis (DVT), having experienced bevacizumab treatment or platinum-based chemotherapy more likely suffered from PE (P < 0.05). The multivariate analysis revealed that high D-dimer, chemotherapy, DVT, stages III to IV, adenocarcinoma were independent risk factors associated with PE (P < 0.05). The overall survival time of patients in case group was significantly shorter than that in the control group with a median survival duration being 10.5 months (95%CI, 8.95-12.05) and 16.8 months (95%CI, 14.62-18.98), respectively, (P < 0.01). CONCLUSIONS: High D-dimer, chemotherapy, DVT, stages III to IV and adenocarcinoma might have a positive correlation with PE, meanwhile, PE always predicted a poor prognosis in lung cancer patients.

7.
Curr Drug Targets ; 2020 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-32988351

RESUMO

Pulmonary fibrosis (PF) is the most common chronic, progressive interstitial lung disease, mainly occurring in the elderly, with a median survival of 2-4 years after diagnosis. Its high mortality rate attributes to the delay in diagnosis due to its generic symptoms, and more importantly, to the lack of effective treatments. MicroRNAs (miRNAs) are a class of small non-coding RNAs that involve in many essential cellular processes, including extracellular matrix remodeling, alveolar epithelial cell apoptosis, epithelial-mesenchymal transition, etc. We summarized the dysregulated miRNAs in TGF-ß signaling pathway-mediated PF in recent years with dual effects, such as anti-fibrotic let-7 family and pro-fibrotic miR-21 members. Therefore, this review will set out the latest application of miRNAs to provide a new direction for PF treatment.

8.
Curr Pharm Des ; 2020 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-32940173

RESUMO

Edema is a gradually accumulation of fluid in the interstitial tissues or luminal cavities, which is regulated by ion transport pathways and reflects a dysfunction of fluid and salt homeostasis. Increasing evidence suggests that some herbal monomers significantly reduce organ/tissue edema. In this review, we briefly summarized the electrolyte permeability involved in pathomechanisms of organ edema, and the benefits of herbal monomers on ionic transport machinery, including Na+ -K+ -ATPase, Na+ and Clchannels, Na+ -K+ -2Clco-transporter, etc. The pharmaceutical relevance is implicated for developing advanced strategies to mitigate edematous disorders. In conclusion, the natural herbal monomers regulate electrolyte permeability in many edematous disorders, and further basic and clinical studies are needed.

9.
Thorac Cancer ; 11(11): 3388-3390, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32986288

RESUMO

Lymphangiomatosis is a rare, benign, hyperproliferative hamartoma composed of dilated lymphatic vessels. Cystic lymphangioma (CL) in the chest wall in an adult patient is rare, but we focus on this type of patient in our present case study. A 54-year-old female patient with a painless mass in her chest wall went without treatment for two years following diagnosis. After consenting to treatment, Doppler color flow imaging (DCFI), chest CT, and MRI revealed a cystic lesion with multiple thin septula in the left chest. Surgical resection was performed, and histopathological examination identified a cystic lymphangioma. The patient did not experience recurrence during the follow-up period.

10.
Medicine (Baltimore) ; 99(39): e22218, 2020 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-32991414

RESUMO

BACKGROUND: Chlorhexidine and silver sulfadiazine coated central venous catheters (CSS-CVC) may cause loss of antimicrobial efficacy due to friction between the CVC surface and sheer stress caused by the blood flow. Therefore, the aim of this study was to investigate the antibacterial efficacy of CSS-CVC at various flow rates using a bloodstream model. METHODS: Each CVC was subjected to various flow rates (0.5, 1, 2, and 4 L/min) and wear-out times (0, 24, 48, 72, 96, and 120 hours), and the optical density (OD) 600 after a Staphylococcus aureus incubation test was used to determine the antibacterial effect of CSS-CVC. RESULTS: In the 0.5 L/min group, there was no significant change in the OD600 value up to 120 hours compared with the baseline OD600 value for CSS-CVC (P > .467). However, the OD600 values of CSS-CVC in the 1 L/min (P < .001) and 2 L/min (P < .001) groups were significantly reduced up to 72 hours, while that in the 4 L/min (p < 0.001) group decreased rapidly up to 48 hours. CONCLUSION: This study suggests that there is a doubt whether sufficient antibacterial function can be maintained with prolonged duration of catheter placement.


Assuntos
Anti-Infecciosos Locais/farmacologia , Velocidade do Fluxo Sanguíneo , Cateterismo Venoso Central/instrumentação , Clorexidina/farmacologia , Sulfadiazina de Prata/farmacologia , Antibacterianos/farmacologia , Anti-Infecciosos Locais/administração & dosagem , Infecções Relacionadas a Cateter/prevenção & controle , Cateteres Venosos Centrais/normas , Clorexidina/administração & dosagem , Humanos , Modelos Biológicos , Projetos Piloto , Sulfadiazina de Prata/administração & dosagem
13.
Curr Pharm Des ; 2020 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-32842936

RESUMO

Steady and continuous supply of oxygen is important for humans, since an excess or deficiency in oxygen levels may result in the death of cells, tissue, or the organism. As a mechanical barrier against pathogens, the respiratory epithelium is always exposed to hypoxia in some detrimental external environment and/or pathologic state. The barrier function is accordingly impaired as a result of disrupted cell composition ratio, ion transport, and tight junctions in a hypoxia-inducible factor dependent or independent way. Hypoxia has been identified as an element of the primary or secondary pathogenic factors of many respiratory diseases, but the relationship between hypoxia and epithelial barrier dysfunction is not fully understood. Thus we summarized recent researches on epithelial barrier dysfunction induced by hypoxia in respiratory system, aiming to explore the possible therapeutic targets in hypoxia related respiratory system diseases.

14.
Artigo em Inglês | MEDLINE | ID: mdl-32805933

RESUMO

With the growing development of the Internet of Things, organic photovoltaic (OPV) cells are highly desirable for indoor applications because of the unique features of light weight, flexibility, and coloration. Emission spectra of the commonly used indoor light sources are much narrower with lower light intensity as compared to the standard solar spectrum. High tunability in optical absorption, insensitivity to series resistance and the active layer thickness, and mild operating conditions make indoor OPV cells promising as a practically relevant technology. Currently, the OPV module has obtained a power conversion efficiency of over 20%, with excellent stability under indoor conditions. However, at the present stage, the device physics investigations and material design strategies developed in an OPV cell for indoor applications lag behind those for outdoor applications. In particular, the emerging characterizations in photovoltaic measurements have severely affected the reliability of reports. This Spotlight on Applications highlights these opportunities and challenges of OPV cells for indoor applications and reviews the recent progress in indoor OPV cells. In addition, we summarize some studies related to accurate measurement and provide some recommendations.

15.
J Am Chem Soc ; 142(38): 16346-16356, 2020 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-32841013

RESUMO

The metal-organic framework (MOF) HKUST-1 with a tbo topology serves as an archetypal tunable and isoreticular framework platform for targeting desired applications, but the design and synthesis of tbo-covalent organic frameworks (COFs) remains a formidable challenge. Here we demonstrate the successful use of reticular chemistry as an appropriate strategy for the design and deliberate construction of COFs with a tbo topology. The judicious selection of the perquisite planar building blocks, 4-connected square tetramine of porphyrin and 3-connected trigonal trialdehydes of triphenylamine, allows the condensation of two tbo-COFs, the first examples of COFs with a tbo topology. The resulting COFs both crystallize in the cubic Pm3̅ space group and adopt a non-interpenetrated open framework, in which each tritopic linker connects to three square units forming a truncated Td-octahedron and occupies the alternating triangular faces of the truncated octahedron. Owing to the presence of two different types of photoredox-active moieties, the two COFs can be efficient heterogeneous photocatalysts for the oxidative hydroxylation of arylboronic acids and the reductive defluoroalkylation of trifluoromethyl aromatics with alkenes. The present finding will provide an impetus to examine the potential of tbo-COFs as a new platform for engineering multifunctional materials via expansion and functionalization of building blocks.

17.
Chin Med J (Engl) ; 133(17): 2020-2026, 2020 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-32810047

RESUMO

BACKGROUND: Youzhi artificial intelligence (AI) software is the AI-assisted decision-making system for diagnosing skin tumors. The high diagnostic accuracy of Youzhi AI software was previously validated in specific datasets. The objective of this study was to compare the performance of diagnostic capacity between Youzhi AI software and dermatologists in real-world clinical settings. METHODS: A total of 106 patients who underwent skin tumor resection in the Dermatology Department of China-Japan Friendship Hospital from July 2017 to June 2019 and were confirmed as skin tumors by pathological biopsy were selected. Dermoscopy and clinical images of 106 patients were diagnosed by Youzhi AI software and dermatologists at different dermoscopy diagnostic levels. The primary outcome was to compare the diagnostic accuracy of the Youzhi AI software with that of dermatologists and that measured in the laboratory using specific data sets. The secondary results included the sensitivity, specificity, positive predictive value, negative predictive value, F-measure, and Matthews correlation coefficient of Youzhi AI software in the real-world. RESULTS: The diagnostic accuracy of Youzhi AI software in real-world clinical settings was lower than that of the laboratory data (P < 0.001). The output result of Youzhi AI software has good stability after several tests. Youzhi AI software diagnosed benign and malignant diseases by recognizing dermoscopic images and diagnosed disease types with higher diagnostic accuracy than by recognizing clinical images (P = 0.008, P = 0.016, respectively). Compared with dermatologists, Youzhi AI software was more accurate in the diagnosis of skin tumor types through the recognition of dermoscopic images (P = 0.01). By evaluating the diagnostic performance of dermatologists under different modes, the diagnostic accuracy of dermatologists in diagnosing disease types by matching dermoscopic and clinical images was significantly higher than that by identifying dermoscopic and clinical images in random sequence (P = 0.022). The diagnostic accuracy of dermatologists in the diagnosis of benign and malignant diseases by recognizing dermoscopic images was significantly higher than that by recognizing clinical images (P = 0.010). CONCLUSION: The diagnostic accuracy of Youzhi AI software for skin tumors in real-world clinical settings was not as high as that of using special data sets in the laboratory. However, there was no significant difference between the diagnostic capacity of Youzhi AI software and the average diagnostic capacity of dermatologists. It can provide assistant diagnostic decisions for dermatologists in the current state.

18.
Dalton Trans ; 2020 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-32697202

RESUMO

The development of ultrathin two-dimensional metal-organic framework nanosheets (2D MONs) has recently attracted increasing interest due to their extensive application potential originating from their ultrathin thickness, large surface area, and innumerable accessible surface-active sites. In this frontier article, we highlight the recent developments of 2D MONs for highly efficient heterogeneous catalysts; they can be grouped into three sections in terms of their functions: (i) as catalysts capable of showing outstanding intrinsic reactivity, (ii) as support materials for prevalent catalysts, and (iii) as catalysts with multifunctional catalytic activity for diverse organic transformations. In addition, the present challenges and future opportunities in this field are also discussed.

19.
Zhongguo Fei Ai Za Zhi ; 23(7): 568-572, 2020 Jul 20.
Artigo em Chinês | MEDLINE | ID: mdl-32702790

RESUMO

BACKGROUND: Preoperative anxiety/depression can bring physical and mental harm to the patients with lung cancer. There is little study on whether hospital waiting time before surgery can increase the psychological burden of patients with lung cancer. The aim of this study was to investigate the preoperative anxiety and depression of patients with lung cancer in our hospital, and to analyze the related influencing factors. METHODS: A total of 135 lung cancer inpatients in the Department of Thoracic Surgery of Beijing Friendship Hospital were studied. Their general information and anxiety/depression were recorded by general questionnaire, Zung self-rating anxiety scale (SAS) and self-rating depression scale (SDS). RESULTS: The score of SAS was 36.25 (30.00, 42.50) on the day of admission, and 37.50 (31.25, 43.75) on one day before operation. The score of self-rating depression scale (SDS) was 46.25 (40.00, 52.50) on the day of admission, and 47.50 (41.25, 53.75) on one day before operation. Compared with the Chinese norm, there were 0 patient suffered from anxiety on the day of admission, and one day before operation. There were 2 patients suffered from mild anxiety; 6 patients suffered from mild depression on the day of admission, and this number went up to 8 on the day before operation. Single factor analysis showed that the hospital waiting time before surgery was positively correlated with preoperative anxiety and depression, and the results were statistically significant (P<0.05). The generalized linear model analysis showed that other factors such as knowledge, gender, age and marital status had no significant correlation with preoperative anxiety and depression. CONCLUSIONS: The occurrence of preoperative anxiety in hospitalized patients with lung cancer is positively correlated to the hospital waiting time before surgery. The longer they stayed in the hospital before operation, the greater their risk of anxiety/depression. So medical staff should actively focus on the psychological condition of the patients with lung cancer, and it is strongly recommended that patients complete preoperative examination and preparation in the outpatient department, in order to reduce the waiting time before operation and reduced the risk of adverse psychological problems such as anxiety and depression.

20.
Chem Soc Rev ; 49(17): 6248-6272, 2020 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-32724943

RESUMO

Covalent organic frameworks (COFs) are constructed using reticular chemistry with the building blocks being connected via covalent bonds and have emerged as a new series of porous materials for multitudinous applications. Most COFs reported to date are achiral, and only a small fraction of COFs with chiral nature are reported. This review covers the recent advances in the field of chiral COFs (CCOFs), including their design principles and synthetic strategies, structural studies, and potential applications in asymmetric catalysis, enantioselective separation, and chiral recognition. Finally, we illustrate the remaining challenges and future opportunities in this field.

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