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1.
Dis Markers ; 2020: 1075942, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32025275

RESUMO

Background: Ovarian cancer is the 5th leading cause of death of women due to cancer in the United States. Although carbohydrate antigen 125 has a moderate diagnostic utility, the phenomenon of false-positive exists. As novel effective biomarkers, some single microRNAs (miRNAs) have diagnostic values for ovarian cancer, but the results lack consistency. In order to precisely and comprehensively assess the diagnostic value of single miRNAs for ovarian cancer, a meta-analysis is performed. Methods: Articles concerning the diagnostic value of single miRNAs for ovarian cancer were searched from databases. The pooled sensitivity (SEN), specificity (SPE), positive likelihood ratio (PLR), negative likelihood ratio (NLR), and diagnostic odds ratio (DOR) with the corresponding 95% confidence interval (CI) were calculated. Area under curve (AUC) of the summary receiver-operating characteristic (SROC) curve was also calculated. Results: In total, 22 studies including 8 kinds of single miRNAs were enrolled in this paper (6 studies for miR-200c, 3 studies for miR-200a and miR-200b, and 2 studies for miR-205, miR-145, miR-141, miR-429, and miR-125b). For miR-200c, the pooled SEN and SPE were, respectively, 0.768 (95% CI: 0.722-0.811) and 0.680 (95% CI: 0.624-0.732); the pooled PLR and NLR were, respectively, 2.897 (95% CI: 1.787-4.698) and 0.340 (95% CI: 0.276-0.417); the pooled DOR was 8.917 (95% CI: 4.521-17.587); and AUC of SROC curve was 0.815. For miR-200a, the pooled SEN and SPE were, respectively, 0.759 (95% CI: 0.670-0.833) and 0.717 (95% CI: 0.627-0.795); the pooled PLR and NLR were, respectively, 3.129 (95% CI: 0.997-9.816) and 0.301 (95% CI: 0.207-0.437); the pooled DOR was 11.323 (95% CI: 3.493-36.711); and AUC of SROC curve was 0.857. For miR-200b, the pooled SEN and SPE were, respectively, 0.853 (95% CI: 0.776-0.912) and 0.775 (95% CI: 0.690-0.846); the pooled PLR and NLR were, respectively, 4.327 (95% CI: 0.683-27.415) and 0.225 (95% CI: 0.081-0.625); the pooled DOR was 19.678 (95% CI: 2.812-137.72); and AUC of SROC curve was 0.90. For miR-205, miR-145, miR-141, miR-429, and miR-125b, each diagnostic value should be interpreted cautiously because only two studies were included. Conclusions: miR-200c, miR-200a, and miR-200b can be useful diagnostic biomarkers for ovarian cancer. More related studies are needed for miR-205, miR-145, miR-141, miR-429, and miR-125b.

2.
J Immunol Res ; 2019: 7024905, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31737687

RESUMO

Objective: Asthma is a syndrome that incorporates many immune phenotypes. The immunologic effects of subcutaneous immunotherapy (SCIT) exerts on allergic asthma remain still largely unknown. Here, we investigated the effects of SCIT on cytokine production and peripheral blood levels of lymphocyte subtypes in children with mite-induced moderate and severe allergic asthma. Methods: The study included 60 kids with mite-induced allergic asthma from 5 to 10 years old. All subjects had received antiasthmatic pharmacologic for 3 months at baseline. Half of the children were treated with SCIT combined with pharmacologic treatment named the SCIT group and the other half only with pharmacologic therapy named the no-SCIT group. Total asthma symptom score (TASS) and total medication score (TMS) were recorded. Flow cytometry was used to identify lymphocyte subtypes: type 2 innate lymphocytes (ILC2s), type 1 (Th1) and type 2 (Th2) helper T cells, T helper 17 (Th17) cells, and regulatory T (Treg) cells. ELISA, flow cytometry, and cytometric bead array were used to assess cytokines IL-13, IFN-γ, IL-4, IL-17, and TGF-ß, at baseline and 3 and 6 months after study treatment in both groups of patients. Results: Both groups can significantly improve clinical symptoms in children with asthma. SCIT can significantly reduce asthma medication after 6 months of treatment. SCIT induced a significantly higher and progressive reduction in ILC2 percentage and IL-13 levels after 3 and 6 months of treatment compared with baseline and compared with no-SCIT patients. Significant differences were detected in the Th1/Th2 cell ratio and IFN-γ/IL-4 cytokine ratio between groups after 6 months of treatment. Similarly, the Th17/Treg ratio and IL-17/TGF-ß ratio in the SCIT group were much lower than those in the no-SCIT group after 3-6 months of treatment. Conclusion: SCIT is a promising option to reduce the percentage of ILC2 and regulate Th1/Th2 and Th17/Treg immune balance in the peripheral blood of children with asthma.

3.
J Reprod Immunol ; 132: 29-34, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30861482

RESUMO

OBJECTIVE: Abnormal changes in immune-mediated inflammation contribute to the pathogenesis of preeclampsia (PE). We aim to investigate the value of systemic immune inflammation indices-neutrophil-lymphocyte ratio (NLR) and monocyte-lymphocyte ratio (MLR)-to identify and evaluate the prognosis of patients with PE. METHODS: This study reviewed clinical records of 367 PE patients (162 with mild PE and 205 with severe PE), in addition to a control group of 172 normal pregnancies. Blood cell counts were performed at the first diagnosis of PE, and NLR and MLR were calculated by absolute cell count. RESULTS: Absolute neutrophil, lymphocyte, and monocyte counts and NLR and MLR values in PE were significantly different from controls, although monocyte counts did not significantly differ between mild and severe PE. Receiver operating characteristics curve (ROC) analysis showed NLR and MLR had better diagnostic accuracy in distinguishing PE from controls [NLR area under the curve (AUC) = 0.70; MLR AUC = 0.78]. Further, NLR was the best predictor of disease severity (AUC = 0.71). Cutoff values of NLR > 4.198 or MLR > 0.325 for control and PE groups or a cutoff value of NLR > 4.182 for PE groups indicated that patients were more likely to encounter preterm delivery, have shorter admission-to-delivery interval, and develop maternal and neonatal complications. CONCLUSION: Secondary analyses of white blood cell differential count parameters effectively evaluate the systemic inflammatory/immune state. Compared with absolute cell counts, NLR and MLR offer more effective indicators of clinical assessment, disease severity evaluation, and prognosis evaluation of PE.

4.
Clin Rev Allergy Immunol ; 56(2): 129-138, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-27444490

RESUMO

The gold standard for diagnosing asthma in children is based on clinical history of respiratory symptoms, physical examination, and respiratory function testing. Recent advances indicate that a non-invasive measure of airway inflammation, fractional exhaled nitric oxide (FeNO), provides objective data for use in asthma diagnosis. However, the diagnostic performance of FeNO in children with asthma has not been clearly defined. This systematic review and meta-analysis aimed to evaluate the diagnostic accuracy of FeNO in the clinical determination of asthma in children. Databases of PubMed, the Cochrane Library, EMBASE, MEDION, and Web of Science were searched for relevant articles through March 31, 2016. A bivariate model was used for pooling estimates of sensitivity, specificity, diagnostic odds ratio (DOR), and area under the summary receiver operating curves (SROC) as the main diagnostic measures. In total, eight studies met the inclusion criteria, which included 2933 subjects. The pooled estimates of sensitivity, specificity, and DOR for the detection of asthma in children were 0.79 [95 % confidence interval (CI), 0.64-0.89], 0.81 (95 % CI, 0.66-0.90), and 16.52 (95 % CI, 7.64-35.71). The SROC was 0.87 (95 % CI, 0.84-0.90). In brief, FeNO achieves a moderate diagnostic performance in the detection of asthma in children.


Assuntos
Asma/diagnóstico , Asma/metabolismo , Expiração , Óxido Nítrico/metabolismo , Criança , Pré-Escolar , Humanos , Óxido Nítrico/análise , Viés de Publicação , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Espirometria/métodos , Espirometria/normas
5.
Protein Pept Lett ; 26(3): 184-191, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30526452

RESUMO

BACKGROUND: The dust mite Dermatophagoides farinae is a common worldwide cause of indoor allergies induced by its proteins, including the mid-tier allergen Der f 7. OBJECTIVE: To identify conformational epitopes in Der f 7 using mimotope mapping and computational modelling. METHODS: Here, we used standard hybridoma technology to generate 3 new monoclonal antibodies against Der f 7 and performed mimotope mapping by probing a random peptide phage display library. Computational tools, including Minox and the DiscoTope-2.0 Server were used to assess the structure and potential position of antigenic residues within Der f 7. RESULTS: Thirteen mimotopes sharing the common sequence --XX[LST]P[-E][LI]MLPLR[-S]- were identified. Further, computationally-predicted conformational epitopes were found at residues 1-7, 10, 27, 76-81, 92, and 130-133 of Der f 7, and the key amino acids for these epitopes were deduced to be 2P, 3I, 10E, 27E, 78E, 79E, 81I, 130S, and 132E based on the common mimotope sequence. CONCLUSION: We identified Der f 7 peptide mimotopes that may model binding sites for blocking antibodies. These may guide the development of immunotherapy for individuals with hypersensitivity to Der f 7.


Assuntos
Anticorpos Monoclonais , Antígenos de Dermatophagoides , Biblioteca de Peptídeos , Pyroglyphidae , Animais , Anticorpos Monoclonais/química , Anticorpos Monoclonais/imunologia , Antígenos de Dermatophagoides/química , Antígenos de Dermatophagoides/genética , Antígenos de Dermatophagoides/imunologia , Pyroglyphidae/química , Pyroglyphidae/genética , Pyroglyphidae/imunologia
6.
Arch Med Sci ; 14(6): 1348-1354, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30393489

RESUMO

Introduction: Specific immunotherapy is critical for alleviating symptoms associated with house dust mite allergy, such as asthma and rhinitis. However, this approach relies on crude extracts, which are often not of sufficient quality or purity and are not standardized. The use of recombinant allergens may enable safer, more effective treatment. Material and methods: Using our previously constructed plasmids pET28a(+)-Der f 1, pET28a(+)-Der f 2 and pET28b(+)-Der f 4 as templates, the gene fragments coding for the allergens Der f 1, Der f 2 and Der f 4, respectively, of the dust mite Dermatophagoides farinae were amplified by PCR. Next the PCR-amplified DNAs were recovered, cloned into pFastBacHT A, and transformed into Escherichia coli DH10Bac. The resulting vectors were co-transfected into Spodoptera frugiperda Sf9 cells for expression. The recombinant allergens were purified by Ni2+ affinity chromatography, and identified by SDS-PAGE and ELISA. Results: The recombinant allergens were successfully expressed and purified from a baculovirus expression system introduced into Sf9 cells, which were verified as being of the correct predicted molecular weights by SDS-PAGE. Furthermore, the reactivity to recombinant allergens rDer f 1, rDer f 2, and rDer f 4 was 85.2%, 88.9%, and 44.4%, respectively, in 27 children with asthma and D. farinae allergy. Conclusions: Recombinant allergens from dust mites can be successfully generated using a baculovirus-insect expression system. Furthermore, these recombinant allergens can be used to detect mite sensitivity in sera, highlighting their utility in future work to understand and develop treatment for mite allergy.

7.
Int J Immunopathol Pharmacol ; 32: 2058738418804095, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30350752

RESUMO

Allergies to various environmental factors, in particular mite species, represent a considerable healthcare burden with lost productivity resulting from symptoms including asthma, conjunctivitis, rhinitis, and atopic dermatitis. The complexity of mite species and the allergens that they produce complicates diagnosis and treatment; however, the advent of recombinant DNA technologies now allows clinicians to better pinpoint the specific sensitizing agents and creates new opportunities for avoidance or immunotherapy. Here we discuss the advantages and disadvantages of traditional and novel diagnostic and therapeutic platforms, with particular consideration given to multiplex tests able to generate patient-specific allergen profiles. Immunotherapies tailored to such profiles may be safer and more effective than generalized treatments, but many hurdles, including the costs associated with identifying the protein or protein combinations able to exert the safest and most beneficial effects, must be overcome before such therapies can be globally applied.


Assuntos
Acaridae/imunologia , Antígenos de Dermatophagoides/imunologia , Proteínas de Artrópodes/imunologia , Hipersensibilidade/diagnóstico , Hipersensibilidade/imunologia , Testes Imunológicos , Pyroglyphidae/imunologia , Animais , Antígenos de Dermatophagoides/efeitos adversos , Proteínas de Artrópodes/efeitos adversos , Biomarcadores/sangue , Humanos , Hipersensibilidade/sangue , Hipersensibilidade/terapia , Imunoglobulina E/sangue , Imunoglobulina E/imunologia , Imunoterapia/métodos , Valor Preditivo dos Testes , Prognóstico , Fatores de Risco
8.
J Insect Sci ; 18(5)2018 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-30285258

RESUMO

Aquaporin water channel proteins are highly conserved across many diverse species. Some evidence indicates that aquaporins in insects may contribute to insect-related mammalian diseases and inflammation, and thus these proteins may represent viable therapeutic targets. Here, we used RNA sequencing and bioinformatics to identify putative aquaporins from the house dust mite, Dermatophagoides farinae. Six putative aquaporins were identified based on sequence similarity with aquaporins from other species. These putative aquaporins, deposited in GenBank and named DerfAQP1-4 (KY231248, KY231249, KY231250, and KY231251, respectively), DerfAQP5.01, and DerfAQP5.02 (KY231252 and KY231253), were successfully cloned into a bacterial plasmid. The identification of full-length aquaporin sequences from D. farinae provides a foundation for future molecular and biochemical studies of these proteins in D. farinae and related species.


Assuntos
Aquaporinas/genética , Dermatophagoides farinae/genética , Proteínas de Insetos/genética , Alérgenos , Sequência de Aminoácidos , Animais , Aquaporinas/química , Aquaporinas/metabolismo , Dermatophagoides farinae/metabolismo , Perfilação da Expressão Gênica , Proteínas de Insetos/química , Proteínas de Insetos/metabolismo , Filogenia , Alinhamento de Sequência
9.
Int J Mol Med ; 42(6): 3551-3561, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30221673

RESUMO

Aquaporins (AQPs), or water channel proteins, are highly conserved across species. These transmembrane proteins promote water and solute transport across cell membranes. No AQP­related proteins have been identified in mites to date. The present study used transcriptomics (RNA­sequencing) to identify potential AQPs in the mite species Blomia tropicalis. Molecular cloning techniques were then used to obtain the full­length gene sequences encoding these AQP family members, and bioinformatics analyses were used to categorize them based on similarity to AQPs in other species. This approach led to the identification of 5 putative AQP­coding sequences, known as BlotAQP1­5 (GenBank accession numbers: KX655540, KX655541, KX655542, KX655543 and KX655544, respectively), which were indexed into all three subgroups, i.e., AQPs, aquaglyceroporins and superAQPs. To the best of our knowledge, these represent the first known AQPs in any mite species. Further studies are required to investigate their functional roles in water transport and their potential as drug targets.


Assuntos
Aquaporinas/genética , Perfilação da Expressão Gênica , Ácaros/genética , Motivos de Aminoácidos , Sequência de Aminoácidos , Animais , Aquaporinas/química , Aquaporinas/metabolismo , Repetições de Microssatélites/genética , Anotação de Sequência Molecular , Filogenia , Estrutura Terciária de Proteína , Análise de Sequência de DNA , Transcriptoma/genética
10.
Biomed Res Int ; 2018: 7632487, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30046607

RESUMO

This study assessed the changes and clinical significance of microRNA-1 (miR-1) and inflammatory factors in the peripheral blood of children with acute-stage asthma. 100 children with acute-stage asthma (study group) and 100 healthy children (control group) were enrolled. For all enrolled children, the peripheral blood levels of miR-1, interleukin-4 (IL-4), IL-5, IL-8, tumor necrosis factor-alpha (TNF-α), and interferon-γ (IFN-γ) were measured. The relative expression levels of miR-1 and IFN-γ in the peripheral blood of children in the study group were significantly lower than those in the control group, whereas expression levels of IL-4, IL-5, IL-8, and TNF-α were significantly higher. Moreover, these levels changed to a greater extent in patients with severe disease (P < 0.05). Further analyses showed that the miR-1 expression level positively correlated with IFN-γ and negatively correlated with IL-4, IL-5, IL-8, and TNF-α expression levels (P < 0.05). ROC curve analysis to identify diagnostic specificity and sensitivity showed that, for diagnosing exacerbation in asthma, the area under the curve (AUC) for miR-1 was the highest (AUC = 0.900, P < 0.05) of all tested markers; this held true for diagnosing severe asthma as well (AUC = 0.977, P < 0.05). Compared to healthy children, children with acute-stage asthma had a low miR-1 expression level and a Th1/Th2 imbalance in their peripheral blood. The changes were closely related, became more exaggerated with an increase in disease severity, and could be used as auxiliary variables for diagnosing asthma exacerbation and evaluating disease severity.


Assuntos
Asma/diagnóstico , MicroRNAs/metabolismo , Doença Aguda , Asma/imunologia , Asma/metabolismo , Biomarcadores/análise , Estudos de Casos e Controles , Criança , Pré-Escolar , Feminino , Humanos , Interferon gama , Interleucinas/metabolismo , Masculino , Curva ROC , Células Th2 , Fator de Necrose Tumoral alfa
11.
Clin Transl Allergy ; 8: 21, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29946417

RESUMO

Background: Spider mites, including Tetranychus urticae, Panonychus citri, and Panonychus ulmi, are common pests in gardens, greenhouses, and orchards. Exposure, particularly occupational exposure, to these organisms may lead to the development of respiratory or contact allergies. However, the prevalence of sensitivity to spider mites is unclear. Methods: We examined the literature to generate an estimate of the global prevalence of allergies to spider mites. Results: Electronic databases were searched and twenty-three studies reporting the prevalence of sensitivity to spider mites (based on skin prick tests or IgE-based detection systems) in an aggregate total of 40,908 subjects were selected for analysis. The estimated overall rate of spider mite sensitivity was 22.9% (95% CI 19-26.8%). Heterogeneity was high and meta-regression analysis considering variables such as published year, country, number of study subjects, methods for allergen detection (skin prick test, ImmunoCAP, RAST testing, or intradermal test), and mite species revealed no single significant source. Twelve of the 23 studies reported rates of monosensitization (i.e., patients responsive to spider mites but no other tested allergen), yielding a global average of 7% (95% CI 5-9%), hence spider mites represent a unique source of allergens. Conclusions: Spider mites are an important cause of allergic symptoms. However, the publication bias and heterogeneity evident in this study indicate that further trials using standardized detection methods are needed to determine the association of exposure and symptoms as well as the specific patient characteristics that influence developing spider mite sensitivity.

12.
Clin Transl Allergy ; 8: 14, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29719717

RESUMO

House dust mites are small arthropods that produce proteins-found in their feces, body parts, and eggs-that are major triggers of human allergies worldwide. The goal of this review is to describe the current methods used to identify these allergens. A literature search for allergen identification methods employed between 1995 and 2016 revealed multiple techniques that can be broadly grouped into discovery and confirmation phases. The discovery phase employs screening for mite proteins that can bind IgEs in sera from animals or patients allergic to dust mites. The confirmation phase employs biochemical methods to isolate either native or recombinant mite proteins, confirms the IgE binding of the purified allergens, and uses either in vitro or in vivo assays to demonstrate that the purified antigen can stimulate an immune response. The methods used in the two phases are defined and their strengths and weaknesses are discussed. The majority of HDM-allergic patients may respond to just a small subset of proteins, but new protein discovery methods are still warranted in order to develop a complete panel of HDM allergens for component resolved diagnosis and patient-tailored therapies.

13.
Int J Immunopathol Pharmacol ; 32: 2058738418779243, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29809062

RESUMO

Regulation of the immune response in asthma is complex. MicroRNA-126 (miR-126) expression has been implicated in this response, so we sought to determine the clinical significance of miR-126 measured in the peripheral blood. A total of 80 children with acute asthma were selected to participate in the study and were compared to 80 healthy children. The relative circulating miR-126 levels, interleukin (IL)-4 levels, and the Th17 cell percentage in the peripheral blood of children in the case group were significantly higher than those in the control group, while the interferon (IFN)-γ levels and the CD4+CD25+Treg cell percentage were significantly lower than those in the control group. Along with the aggravation of the disease, the relative levels of miR-126 and IL-4 and the percentage of Th17 cells increased gradually, while the IFN-γ levels and the CD4+CD25+Treg cell percentage decreased. The relative level of miR-126 in the peripheral blood of children with asthma was positively correlated with IL-4 and the Th17 cell percentage and was negatively correlated with IFN-γ levels, CD4+CD25+Treg cell percentage and lung function indicators. The relative level of miR-126 was correlated with the Th17 cell percentage in the peripheral blood, forced vital capacity (FVC), and forced expiratory flow (FEF)75% of the children with asthma. The relative levels of miR-126 and IL-4 and the Th17 cell percentage were positively correlated with the severity of the asthma, while IFN-γ levels and the CD4+CD25+Treg cell percentage were negatively correlated with the severity of the asthma. CD4+CD25+Treg cell percentage and relative miR-126 levels were of the most predictive value in the diagnosis of asthma. Our findings show that the overexpression of miR-126 in acute asthma is correlated with signs of immune imbalance and is predictive of the severity of the disease, suggesting that it could be used as a potential serological marker for asthma diagnosis and evaluation.


Assuntos
Asma/sangue , MicroRNA Circulante/sangue , MicroRNAs/sangue , Células Th17/imunologia , Asma/diagnóstico , Asma/genética , Asma/imunologia , Estudos de Casos e Controles , Criança , Pré-Escolar , MicroRNA Circulante/genética , Estudos Transversais , Progressão da Doença , Feminino , Humanos , Interferon gama/sangue , Interleucina-4/sangue , Pulmão/imunologia , Pulmão/fisiopatologia , Masculino , MicroRNAs/genética , Valor Preditivo dos Testes , Índice de Gravidade de Doença , Linfócitos T Reguladores/imunologia , Linfócitos T Reguladores/metabolismo , Células Th17/metabolismo , Regulação para Cima
14.
Braz J Med Biol Res ; 51(5): e6213, 2018 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-29561952

RESUMO

Dermatophagoides farinae (Der f), one of the main species of house dust mites, produces more than 30 allergens. A recently identified allergen belonging to the alpha-tubulin protein family, Der f 33, has not been characterized in detail. In this study, we used bioinformatics tools to construct the secondary and tertiary structures and predict the B and T cell epitopes of Der f 33. First, protein attribution, protein patterns, and physicochemical properties were predicted. Then, a reasonable tertiary structure was constructed by homology modeling. In addition, six B cell epitopes (amino acid positions 34-45, 63-67, 103-108, 224-230, 308-316, and 365-377) and four T cell epitopes (positions 178-186, 241-249, 335-343, and 402-410) were predicted. These results established a theoretical basis for further studies and eventual epitope-based vaccine design against Der f 33.


Assuntos
Alérgenos/química , Antígenos de Dermatophagoides/química , Dermatophagoides farinae/química , Epitopos de Linfócito B/química , Epitopos de Linfócito T/química , Tubulina (Proteína)/química , Alérgenos/genética , Alérgenos/imunologia , Animais , Antígenos de Dermatophagoides/genética , Antígenos de Dermatophagoides/imunologia , Biologia Computacional , Dermatophagoides farinae/genética , Dermatophagoides farinae/imunologia , Mapeamento de Epitopos , Epitopos de Linfócito B/genética , Epitopos de Linfócito T/genética , Estrutura Molecular , Estrutura Terciária de Proteína , Análise de Sequência de Proteína , Tubulina (Proteína)/genética , Tubulina (Proteína)/imunologia
15.
Int J Immunopathol Pharmacol ; 32: 394632017750997, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29357711

RESUMO

Airway epithelium cells are the first line of defense against airborne allergens. When cultured, epithelial cells can be exposed to various allergens, providing an ideal model to investigate allergic disorders. This study sought to characterize the profile of long noncoding (lnc) RNAs, which can regulate gene expression and exert functions in diverse cellular processes, in airway epithelial cells exposed to house dust mite allergens. NCI-H292 cells were exposed to house dust mite extract for 24 h. RNA expression was profiled in exposed and unexposed cells. There were 270 lncRNAs that were differentially expressed (fold change ≥ 2, P < 0.05) in NCI-H292 cells after stimulation with Dermatophagoides farinae (house dust mite) extracts. Furthermore, 119 lncRNAs and 22 messenger RNAs were co-expressed. Gene Ontology analysis showed that these under-regulated and up-regulated lncRNAs were associated with biological process, cellular component, and molecular function. After bioinformatic analysis of significantly regulated signaling pathways, we found these lncRNAs may target 16 gene pathways, including glycolysis, axon guidance, ErbB signaling, and mitogen-activated protein kinases (MAPK) signaling. The identification of differentially regulated lncRNAs in NCI-H292 cells after stimulation with Dermatophagoides farinae extracts, as well as their target gene pathways, can provide insight to the etiology and pathogenesis of allergy.


Assuntos
Alérgenos/biossíntese , Dermatophagoides farinae/metabolismo , RNA Longo não Codificante/biossíntese , Mucosa Respiratória/imunologia , Transcriptoma/fisiologia , Alérgenos/genética , Alérgenos/imunologia , Animais , Antígenos de Dermatophagoides/biossíntese , Antígenos de Dermatophagoides/genética , Antígenos de Dermatophagoides/imunologia , Linhagem Celular , Dermatophagoides farinae/genética , Dermatophagoides farinae/imunologia , Redes Reguladoras de Genes/fisiologia , Humanos , RNA Longo não Codificante/genética , RNA Longo não Codificante/imunologia , Mucosa Respiratória/metabolismo
16.
Mol Med Rep ; 17(1): 1807-1812, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29257224

RESUMO

House dust mite allergens can cause allergic diseases, including asthma, atopic dermatitis and rhinitis. Der f 20 is a novel allergen of Dermatophagoides farina (Der f), which is an arginine kinase. In the present study, the B­cell and T­cell epitopes of Der f 20 were predicted. The protein attribution, patterns, physicochemical properties and secondary structure of Der f 20 were also predicted. Der f 20 is a member of the ATP:guanido phosphotransferase family and contains a phosphagen kinase pattern. Using homology modeling, the present study constructed a reasonable tertiary structure of Der f 20. Using BcePred, ABCpred, BCPred and BPAP systems, B­cell epitopes at 20­25, 41­49, 111­118, 131­141, 170­174 and 312­321 were predicted. Using NetMHCIIpan­3.0 and NetMHCII­2.2, T­cell epitopes were predicted at 194­202, 239­247 and 274­282. These results provide a theoretical basis for the design off Der f 20 epitope­based vaccines.


Assuntos
Antígenos de Dermatophagoides/química , Epitopos de Linfócito B/química , Epitopos de Linfócito T/química , Pyroglyphidae/química , Sequência de Aminoácidos , Animais , Antígenos de Dermatophagoides/imunologia , Epitopos de Linfócito B/imunologia , Epitopos de Linfócito T/imunologia , Modelos Moleculares , Conformação Proteica , Estrutura Secundária de Proteína , Pyroglyphidae/imunologia , Homologia Estrutural de Proteína
17.
Braz. j. med. biol. res ; 51(5): e6213, 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-889085

RESUMO

Dermatophagoides farinae (Der f), one of the main species of house dust mites, produces more than 30 allergens. A recently identified allergen belonging to the alpha-tubulin protein family, Der f 33, has not been characterized in detail. In this study, we used bioinformatics tools to construct the secondary and tertiary structures and predict the B and T cell epitopes of Der f 33. First, protein attribution, protein patterns, and physicochemical properties were predicted. Then, a reasonable tertiary structure was constructed by homology modeling. In addition, six B cell epitopes (amino acid positions 34-45, 63-67, 103-108, 224-230, 308-316, and 365-377) and four T cell epitopes (positions 178-186, 241-249, 335-343, and 402-410) were predicted. These results established a theoretical basis for further studies and eventual epitope-based vaccine design against Der f 33.


Assuntos
Animais , Tubulina (Proteína)/química , Alérgenos/química , Epitopos de Linfócito T/química , Epitopos de Linfócito B/química , Dermatophagoides farinae/química , Antígenos de Dermatophagoides/química , Tubulina (Proteína)/genética , Tubulina (Proteína)/imunologia , Alérgenos/genética , Alérgenos/imunologia , Estrutura Molecular , Estrutura Terciária de Proteína , Mapeamento de Epitopos , Epitopos de Linfócito T/genética , Epitopos de Linfócito B/genética , Biologia Computacional , Análise de Sequência de Proteína , Dermatophagoides farinae/genética , Dermatophagoides farinae/imunologia , Antígenos de Dermatophagoides/genética , Antígenos de Dermatophagoides/imunologia
18.
Clin Transl Allergy ; 7: 41, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29209493

RESUMO

Background: Atopic dermatitis (AD) can occur after contact with aeroallergens like house dust mites, pollen, and animal dander. Despite its controversial diagnostic value, the atopy patch test (APT) has been used as an important tool in the diagnosis of AD caused by house dust mites. Here, we present a meta-analysis comparing APT to the common skin prick test (SPT) in the diagnosis of mite-induced AD. Methods: A structured search was performed using online databases and bibliographies published as of April 30, 2017. All studies evaluating the accuracy of APT and SPT in the diagnosis of mite-induced atopic eczema/dermatitis syndrome were selected, appraised, and data was extracted. Results: Ten studies were identified for inclusion in our analysis. Meta-analysis revealed that the pooled sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, and diagnostic odds ratios for APT were 0.54 (95% CI 0.42-0.66), 0.72 (95% CI 0.56-0.85), 1.97 (95% CI 1.20-3.23), 0.63 (95% CI 0.48-0.83), and 3.12 (95% CI 1.53-6.39). The area under the summary receiver operating characteristic curve was 0.65 (95% CI 0.61-0.69). Conclusions: Our analysis indicates that APT is a useful tool in the screening of mite-induced AD, although this conclusion must be interpreted cautiously due to high heterogeneity among the included studies.

19.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 33(8): 1128-1132, 2017 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-28871956

RESUMO

Objective To screen the possible linear epitopes of major and mid-potency allergens in Dermatophagoides farinae (Der f1, Der f2, Der f4, Der f5 and Der f7). Methods Short peptides were synthesized on the basis of the amino acid sequences in active fraction of Der f1, Der f2, Der f4, Der f5 and Der f7. Each peptide had eight amino acids in length and seven of them were overlapped with each other. Put these peptides to the chip to build microarrays that would have immunoreaction with human serum IgE. Then the chips were scanned to analyze the data. Results A total of 1128 short peptides from the above five groups of allergens were synthesized, and the microarray chips were constructed. Six serum samples from children who were allergic to Dermatophagoides farinae were mixed and added to the microarray chips. The chips were scanned and analyzed, and the results showed that Der f1 had four epitopes (46-53aa, 71-78aa, 99-110aa and 179-186aa), that Der f2 had three epitopes (15-22aa, 80-89aa and 106-113aa), that Der f 4 had six epitopes (69-82aa, 107-116aa, 225-232aa, 261-268aa, 355-365aa and 483-496aa), that Der f5 had one epitope (102-109aa), and Der f7 had three epitopes (32-39aa, 52-64aa and 100-107aa). Conclusion We identified the linear epitopes of Der f1, Der f2, Der f4, Der f5 and Der f7.


Assuntos
Alérgenos/imunologia , Epitopos , Imunoglobulina E/imunologia , Análise Serial de Proteínas/métodos , Pyroglyphidae/imunologia , Animais , Antígenos de Dermatophagoides/imunologia , Proteínas de Artrópodes/imunologia , Cisteína Endopeptidases/imunologia , Humanos
20.
Mol Med Rep ; 16(4): 4903-4908, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28791346

RESUMO

Aquaporins (AQP) are proteins that form channels to facilitate the movement of water across cell membranes in plants, bacteria and animals. Insect AQPs are indispensable for cellular water management under stress, including dehydration and cold. To better understand the biological significance of molecular evolution of gene sequences, followed by structural and functional specialization, the present study used ClustalX2.1, MEGA7.0, Jalview and Mesquite software to build an insect AQP phylogenetic tree and visualize the evolutionary associations among insect AQPs. It was demonstrated that 45 AQPs were classified as four major paralogs with each amino acid sequence containing two conserved NPA (Asp­Pro­Ala) motifs located in the center and C­terminal domains, and other residues conserved within the paralogous groups, however not among them. All these differences in amino acid content may affect the structure, function and classification of the AQPs. The findings provide a basis for further study to understand insect AQPs through sequence comparison, structure and predicted function.


Assuntos
Aquaporinas/genética , Genes de Insetos , Insetos/classificação , Insetos/genética , Filogenia , Sequência de Aminoácidos , Animais , Sequência Conservada , Evolução Molecular , Análise de Sequência de DNA , Especificidade da Espécie
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